LOCUS       DAZPDE010000001        65969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPDE010000001 DAZPDE010000000
VERSION     DAZPDE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.001"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(65..544)
                     /gene="folK"
                     /locus_tag="ACHU0J_000001"
     CDS             complement(65..544)
                     /gene="folK"
                     /locus_tag="ACHU0J_000001"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1214885.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(541..1938)
                     /gene="pcnB"
                     /locus_tag="ACHU0J_000002"
     CDS             complement(541..1938)
                     /gene="pcnB"
                     /locus_tag="ACHU0J_000002"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1214886.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1998..2894)
                     /gene="gluQRS"
                     /locus_tag="ACHU0J_000003"
     CDS             complement(1998..2894)
                     /gene="gluQRS"
                     /locus_tag="ACHU0J_000003"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1214887.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2961..3416)
                     /gene="dksA"
                     /locus_tag="ACHU0J_000004"
     CDS             complement(2961..3416)
                     /gene="dksA"
                     /locus_tag="ACHU0J_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1214888.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3594..4298)
                     /gene="sfsA"
                     /locus_tag="ACHU0J_000005"
     CDS             complement(3594..4298)
                     /gene="sfsA"
                     /locus_tag="ACHU0J_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1214889.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4313..4843)
                     /gene="thpR"
                     /locus_tag="ACHU0J_000006"
     CDS             complement(4313..4843)
                     /gene="thpR"
                     /locus_tag="ACHU0J_000006"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1214890.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4917..7346
                     /gene="hrpB"
                     /locus_tag="ACHU0J_000007"
     CDS             4917..7346
                     /gene="hrpB"
                     /locus_tag="ACHU0J_000007"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1214891.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7542..10076
                     /gene="mrcB"
                     /locus_tag="ACHU0J_000008"
     CDS             7542..10076
                     /gene="mrcB"
                     /locus_tag="ACHU0J_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1214892.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10296..12539
                     /gene="fhuA"
                     /locus_tag="ACHU0J_000009"
     CDS             10296..12539
                     /gene="fhuA"
                     /locus_tag="ACHU0J_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1214893.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12590..13387
                     /gene="fhuC"
                     /locus_tag="ACHU0J_000010"
     CDS             12590..13387
                     /gene="fhuC"
                     /locus_tag="ACHU0J_000010"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1214894.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13387..14277
                     /gene="fhuD"
                     /locus_tag="ACHU0J_000011"
     CDS             13387..14277
                     /gene="fhuD"
                     /locus_tag="ACHU0J_000011"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1214895.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14274..16256
                     /gene="fhuB"
                     /locus_tag="ACHU0J_000012"
     CDS             14274..16256
                     /gene="fhuB"
                     /locus_tag="ACHU0J_000012"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1214896.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16291..17571)
                     /gene="hemL"
                     /locus_tag="ACHU0J_000013"
     CDS             complement(16291..17571)
                     /gene="hemL"
                     /locus_tag="ACHU0J_000013"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1214897.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17737..17784
                     /gene="yadX"
                     /locus_tag="ACHU0J_000014"
     CDS             17737..17784
                     /gene="yadX"
                     /locus_tag="ACHU0J_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1214898.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17796..19217
                     /gene="clcA"
                     /locus_tag="ACHU0J_000015"
     CDS             17796..19217
                     /gene="clcA"
                     /locus_tag="ACHU0J_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1214899.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19241..19306
                     /gene="yadW"
                     /locus_tag="ACHU0J_000016"
     CDS             19241..19306
                     /gene="yadW"
                     /locus_tag="ACHU0J_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1214900.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19299..19643
                     /gene="erpA"
                     /locus_tag="ACHU0J_000017"
     CDS             19299..19643
                     /gene="erpA"
                     /locus_tag="ACHU0J_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1214901.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19690..20313)
                     /locus_tag="ACHU0J_000018"
     CDS             complement(19690..20313)
                     /locus_tag="ACHU0J_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1214902.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20351..21151)
                     /gene="btuF"
                     /locus_tag="ACHU0J_000019"
     CDS             complement(20351..21151)
                     /gene="btuF"
                     /locus_tag="ACHU0J_000019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1214903.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21144..21842)
                     /gene="mtnN"
                     /locus_tag="ACHU0J_000020"
     CDS             complement(21144..21842)
                     /gene="mtnN"
                     /locus_tag="ACHU0J_000020"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1214904.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21926..23443
                     /gene="dgt"
                     /locus_tag="ACHU0J_000021"
     CDS             21926..23443
                     /gene="dgt"
                     /locus_tag="ACHU0J_000021"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1214905.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23573..24997
                     /gene="degP"
                     /locus_tag="ACHU0J_000022"
     CDS             23573..24997
                     /gene="degP"
                     /locus_tag="ACHU0J_000022"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1214906.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25152..26308
                     /gene="cdaR"
                     /locus_tag="ACHU0J_000023"
                     /pseudo
     CDS             25152..26308
                     /gene="cdaR"
                     /locus_tag="ACHU0J_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26397..26783)
                     /locus_tag="ACHU0J_000024"
     CDS             complement(26397..26783)
                     /locus_tag="ACHU0J_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1214907.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27097..27921)
                     /gene="dapD"
                     /locus_tag="ACHU0J_000025"
     CDS             complement(27097..27921)
                     /gene="dapD"
                     /locus_tag="ACHU0J_000025"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1214908.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27952..30624)
                     /gene="glnD"
                     /locus_tag="ACHU0J_000026"
     CDS             complement(27952..30624)
                     /gene="glnD"
                     /locus_tag="ACHU0J_000026"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1214909.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30686..31480)
                     /gene="map"
                     /locus_tag="ACHU0J_000027"
     CDS             complement(30686..31480)
                     /gene="map"
                     /locus_tag="ACHU0J_000027"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1214910.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31848..32573
                     /gene="rpsB"
                     /locus_tag="ACHU0J_000028"
     CDS             31848..32573
                     /gene="rpsB"
                     /locus_tag="ACHU0J_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1214911.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32831..33682
                     /gene="tsf"
                     /locus_tag="ACHU0J_000029"
     CDS             32831..33682
                     /gene="tsf"
                     /locus_tag="ACHU0J_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1214912.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33829..34554
                     /gene="pyrH"
                     /locus_tag="ACHU0J_000030"
     CDS             33829..34554
                     /gene="pyrH"
                     /locus_tag="ACHU0J_000030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1214913.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34846..35403
                     /gene="frr"
                     /locus_tag="ACHU0J_000031"
     CDS             34846..35403
                     /gene="frr"
                     /locus_tag="ACHU0J_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1214914.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35495..36691
                     /gene="ispC"
                     /locus_tag="ACHU0J_000032"
     CDS             35495..36691
                     /gene="ispC"
                     /locus_tag="ACHU0J_000032"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1214915.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36880..37638
                     /gene="ispU"
                     /locus_tag="ACHU0J_000033"
                     /gene_synonym="uppS"
     CDS             36880..37638
                     /gene="ispU"
                     /locus_tag="ACHU0J_000033"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1214916.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37651..38508
                     /gene="cdsA"
                     /locus_tag="ACHU0J_000034"
     CDS             37651..38508
                     /gene="cdsA"
                     /locus_tag="ACHU0J_000034"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1214917.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38520..39872
                     /gene="rseP"
                     /locus_tag="ACHU0J_000035"
     CDS             38520..39872
                     /gene="rseP"
                     /locus_tag="ACHU0J_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1214918.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39902..42334
                     /gene="bamA"
                     /locus_tag="ACHU0J_000036"
     CDS             39902..42334
                     /gene="bamA"
                     /locus_tag="ACHU0J_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1214919.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42456..42941
                     /gene="skp"
                     /locus_tag="ACHU0J_000037"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42456..42941
                     /gene="skp"
                     /locus_tag="ACHU0J_000037"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1214920.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42945..43970
                     /gene="lpxD"
                     /locus_tag="ACHU0J_000038"
     CDS             42945..43970
                     /gene="lpxD"
                     /locus_tag="ACHU0J_000038"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1214921.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44075..44530
                     /gene="fabZ"
                     /locus_tag="ACHU0J_000039"
     CDS             44075..44530
                     /gene="fabZ"
                     /locus_tag="ACHU0J_000039"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1214922.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44534..45322
                     /gene="lpxA"
                     /locus_tag="ACHU0J_000040"
     CDS             44534..45322
                     /gene="lpxA"
                     /locus_tag="ACHU0J_000040"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1214923.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45322..46470
                     /gene="lpxB"
                     /locus_tag="ACHU0J_000041"
     CDS             45322..46470
                     /gene="lpxB"
                     /locus_tag="ACHU0J_000041"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1214924.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46467..47063
                     /gene="rnhB"
                     /locus_tag="ACHU0J_000042"
     CDS             46467..47063
                     /gene="rnhB"
                     /locus_tag="ACHU0J_000042"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1214925.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47100..50582
                     /gene="dnaE"
                     /locus_tag="ACHU0J_000043"
     CDS             47100..50582
                     /gene="dnaE"
                     /locus_tag="ACHU0J_000043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1214926.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50595..51554
                     /gene="accA"
                     /locus_tag="ACHU0J_000044"
     CDS             50595..51554
                     /gene="accA"
                     /locus_tag="ACHU0J_000044"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1214927.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51653..53794
                     /gene="ldcC"
                     /locus_tag="ACHU0J_000045"
     CDS             51653..53794
                     /gene="ldcC"
                     /locus_tag="ACHU0J_000045"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1214928.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53851..54240
                     /locus_tag="ACHU0J_000046"
     CDS             53851..54240
                     /locus_tag="ACHU0J_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1214929.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54305..55603
                     /gene="tilS"
                     /locus_tag="ACHU0J_000047"
     CDS             54305..55603
                     /gene="tilS"
                     /locus_tag="ACHU0J_000047"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1214930.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55652..55912)
                     /gene="rof"
                     /locus_tag="ACHU0J_000048"
     CDS             complement(55652..55912)
                     /gene="rof"
                     /locus_tag="ACHU0J_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1214931.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55899..56099)
                     /locus_tag="ACHU0J_000049"
     CDS             complement(55899..56099)
                     /locus_tag="ACHU0J_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1214932.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56265..56810
                     /locus_tag="ACHU0J_000050"
     CDS             56265..56810
                     /locus_tag="ACHU0J_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1214933.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56807..57229
                     /gene="arfB"
                     /locus_tag="ACHU0J_000051"
     CDS             56807..57229
                     /gene="arfB"
                     /locus_tag="ACHU0J_000051"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR1214934.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57243..57953
                     /gene="nlpE"
                     /locus_tag="ACHU0J_000052"
                     /gene_synonym="cutF"
     CDS             57243..57953
                     /gene="nlpE"
                     /locus_tag="ACHU0J_000052"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1214935.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58108..58932)
                     /locus_tag="ACHU0J_000053"
     CDS             complement(58108..58932)
                     /locus_tag="ACHU0J_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1214936.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58985..60703)
                     /gene="proS"
                     /locus_tag="ACHU0J_000054"
     CDS             complement(58985..60703)
                     /gene="proS"
                     /locus_tag="ACHU0J_000054"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1214937.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60814..61521)
                     /gene="tsaA"
                     /locus_tag="ACHU0J_000055"
     CDS             complement(60814..61521)
                     /gene="tsaA"
                     /locus_tag="ACHU0J_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1214938.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSTPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61518..61922)
                     /gene="rcsF"
                     /locus_tag="ACHU0J_000056"
     CDS             complement(61518..61922)
                     /gene="rcsF"
                     /locus_tag="ACHU0J_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1214939.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62040..62855)
                     /gene="metQ"
                     /locus_tag="ACHU0J_000057"
     CDS             complement(62040..62855)
                     /gene="metQ"
                     /locus_tag="ACHU0J_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1214940.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62895..63548)
                     /locus_tag="ACHU0J_000058"
     CDS             complement(62895..63548)
                     /locus_tag="ACHU0J_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1214941.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63541..64572)
                     /gene="metN"
                     /locus_tag="ACHU0J_000059"
     CDS             complement(63541..64572)
                     /gene="metN"
                     /locus_tag="ACHU0J_000059"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1214942.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64760..65335
                     /gene="gmhB"
                     /locus_tag="ACHU0J_000060"
     CDS             64760..65335
                     /gene="gmhB"
                     /locus_tag="ACHU0J_000060"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1214943.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65699..>65969
                     /locus_tag="ACHU0J_000061"
     rRNA            65699..>65969
                     /locus_tag="ACHU0J_000061"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15606 a        16341 c        18184 g        15838 t
ORIGIN      
        1 ttttattaag gaaagagagg atgtatgatt ttcagaaaat atttatattc gtaatataaa
       61 taaattacca tttgcttaat ttgtcaaatg ctcttgtatg taagacttca cgcaacgtct
      121 ccccatcagg aaacgccagc tccggtgcaa tctcaaacag cggccacagc ataaatccac
      181 gattcttcat atcgtagtgc ggaacggtca ggcgttcggt atttatcact tcattaccaa
      241 acagcatgat atcgagatcc agcgtgcgtg gtccccagcg ttcagcttta cgaacacgac
      301 cctgttgcag ttctatgcgc tgtgtatggt tgagtaactc ttccggggcg ggcgaagttt
      361 caagcgccac ggcggcgttg aggtaatcgg gttgatcttg cggccccagc ggtggggtgc
      421 ggtaaaacga agaaacggca agaatgcggc tttcagggat atcgcctaat gctttcaggg
      481 cagcattgac ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg
      541 tcatgtggta ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac gacgacgcgg
      601 tgacggttct tcatccagct cgttaagcat ccctttttga tctggtggtg cggaaacctg
      661 gaactcaccc caccatttca ccagacgctg cagttcagca ttacgttcaa cttcagctcg
      721 caaggccaac aggtcataag ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg
      781 tttaccctga cgacgggaca tacgcaactg caactgccag atatcgcggg ttaacgtcgt
      841 cagacgtttc gggattgcca gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag
      901 cgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg ccgtctccag
      961 cagtgggtac cagaacatgg cggcaaacaa gaacgccggg ttcacgcgca tatcgttatg
     1021 gatacgcgta tcggtattct tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc
     1081 attttccgtg aagtagcggg taatggtcgg gaacagcggc tggaacagat gatattcaca
     1141 caacagctta taggtttcgt aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa
     1201 caggcgtgcc ggtgggatat cgttcagcag ggtagcgagg cgagggatcg gttctgcggt
     1261 ttccgggctg atgcgcatac ccaatttggc ggcaaaacgt accgcgcgca gcatacgtac
     1321 cggatcttca cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtccttcag
     1381 atccttcata ccaccaacgt aatcacggac ggtaaaatcc gctacgctgt aatacaggct
     1441 gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg
     1501 cagcaacatg ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg
     1561 gtgtccacgg aaggtcgcaa cttcgataat ctccgggcca aacattacat gagccagacg
     1621 gaaacggcga cccaccaggc ggcagttacg gaacagtttg cgcacctgct caggcgtggc
     1681 gttagtggtt acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc
     1741 gccaaccagc caggcttcgt atcccgcttt gttgagcctg tacattacct tcagggcatt
     1801 ttcactgata tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg
     1861 acgggcgact gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt
     1921 agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa
     1981 tagcggctaa tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac
     2041 tcgggcacgg cggtcagcat ccagtttttg acggctgact gaaggatttg ctcgacgctg
     2101 aaatcctgcc agtgtgcttc tgcctgctgc cccagaaatt gaagtgccgc gattagtacc
     2161 gggcgtggat cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct
     2221 tgtggattaa gcgccagcgg cagatgaatg taatctggca ctttccagcc aaaaagctgg
     2281 tacagcggga tttgccttac tgttggttca atcagatcag ccccacgcac tatttctgta
     2341 acgccctgga aatgatcatc aaccacaaca gccaggttgt aggcgaacaa cccatcacgg
     2401 cgatgaatga taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat gcctcgcagc
     2461 aggtcagtaa attgcgtgac cggatgctgc tggcggatac gcactgcggc gttgtctggt
     2521 ccatgatgca acacccggca atgaccgtcg taaataccgc caatgctttg aatacgcgca
     2581 cgcgtacagg tgcagtaata acttagtcct tgttcatgta accaggcgag tgcttcacga
     2641 taggcgtcgt gacgttgcga ttgccagaga acatcgccgt cccagtgcag accgtaatgt
     2701 tccagctggc gcaggatagt ttctgcggca ccgggaactt cacgaggcgg gtcgatatct
     2761 tctatgcgta ccagccagcg accttgccgg gcgcgagcct gcaaatagct gccgagcgcg
     2821 gcgatcagag agccaaaatg aagctcgccg gaaggagagg gggcgaagcg gccaatatac
     2881 tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa
     2941 acgtgatgga acggctgtaa ttagccagcc atctgttttt cgcgaatttc agccagcgtt
     3001 ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accaatttca
     3061 acaccgcagg attcgcagta gccgaaatct tcgtcttcca cttttttcag cgtcttctcg
     3121 atctttttga tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct
     3181 tcctgggctg cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta
     3241 acggtgcgat cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag
     3301 tgcgccagct gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc
     3361 ccagcgatgg cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg
     3421 cttctcctta acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa
     3481 tagcagatgc ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga
     3541 aaagcgtatt tgcgcacgcg accagaatgt aaattaacca gttacttact ttactacaat
     3601 gtaaccggca gtgatttttt aagagccatg ccttcagcag aaatttccgc tttgtaagcc
     3661 agaatttcta cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg
     3721 tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa aataaccgca
     3781 cgctggcctt cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg
     3841 accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg taaccgattt cacttcaata
     3901 tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttcggagccg
     3961 tattttactt cgcttttcag cgagctatag cctgacagtt ctgaaattga ttcattaagg
     4021 atagcctctt tcgtcaacct gttagcccaa agcgtattga cgcaaataaa tgcgccgctc
     4081 tggctttgag ttaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa
     4141 taccagacgg tatcgccagg cgttgcacaa ccggtcatcg cacccgtatt cgggcagtgt
     4201 agcgttaatt cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga
     4261 attagcgtcg cgcgctgtag agggggagaa aattccattg cgacaatcct tgttattgcg
     4321 ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt
     4381 aaagggtgaa ctccgtcacc gcataagacc agttaaaacc cggcggcggg aatgtcaccg
     4441 cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac
     4501 aaccgctgcg ggcagcctgt gaacggagca tattcgccag ctggattaag ccgcgtggag
     4561 gctgacgcat ccctaaccac accacacgcg aacgcagcca ttgtccggcg tcatcaagcg
     4621 tgagtgtgaa accaggttga cgaatccgtc cggctaaaag agaaagcgcc ttctctttct
     4681 ctgcgctcac ttcgcctaaa aatgccagag tcaggtgcaa attatcggcg gcgaccggac
     4741 gtcccgcctc aggtgggaag tgtgtggcgc gccaatggat aatctgttcg cggatttctg
     4801 caggtaagtc gatagcaaag aacagacgtt gcggttcaga catgtgaggc actcggttat
     4861 gaattaccgc gatgctacaa tgtggcgcga agaatgttaa ccctctggag cgttttgtgt
     4921 cgtcgttgcc cgttgctgcc gtcttacctg aattacttac cgctctcgat tgtgcgccgc
     4981 aggtattatt aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc
     5041 tggcgcatcc cggcattaac gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc
     5101 gtaacgtcgc gcaacggctg gcggagctgc ttaacgaaaa gccaggcgat accgttggct
     5161 accggatgcg tgcgcaaaac tgcgtcgggc tgaatacccg cctggaagtg gttaccgaag
     5221 gcgtgctgac gcggatgatc cagcgtgacc cggaactgag cggtgttgga ttggtgatcc
     5281 ttgatgagtt tcatgagcgc agtttgcagg cggatttggc gttggcgctg ttactcgatg
     5341 tgcaacaagg tctgcgtgat gaccttaaac tgctgattat gtcggctacg ctggacaatg
     5401 accgcttgca gcaaatgctg ccggaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc
     5461 cggttgaacg ccgttattta ccgctgcccg cgcatcagcg ttttgacgaa gccgttgcgg
     5521 tagccaccgc cgaaatggtg cgtcaggaaa gtggatcatt actgttattt ttacctggcg
     5581 tcggagaaat tcagcgcgtg caggaacaac tggcttcgcg catcggcagt gatgtggtgc
     5641 tctgcccgct gtatggcgtg ttgtcactga acgatcagcg aaaagcgatc ctcccggcac
     5701 cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt ttaaccatcg
     5761 aaggtattcg tctggtggtg gattgtgccc aggagcgtgt ggcgcgcttt gatccgcgca
     5821 cggggcttac gcggttgatt actcaacgcg ttagtcaggc atccatgacg cagcgtgccg
     5881 ggcgcgccgg gcgtctggag ccgggtatca gcctgcattt aatcgccaaa gaacaagcag
     5941 aacgcgccgc ggcgcaaagt gagccgaaga tcttacaaag cgatctttcc ggtttgctga
     6001 tggaattact gcaatgggga tgcagcgatc cggcgcagat gagctggctg gatcaaccgc
     6061 caacggtgaa tctactggcc gcgaaacgcc tgttacaaat gctgggggcg ctggagggtg
     6121 aacggcttag tgcgcaaggg caaaaaatgg cagcgctggg taacgatccg cgtttagcgg
     6181 caatgctggt taacgcaaag agcgatgacg aagctgctac cgcggcaaaa attgccgcca
     6241 ttctcgaaga gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc
     6301 aaccagcctg gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta cgcggcggtg
     6361 aggcagacag ttcgcttatc gcgccgctac ttgccggggc gtttgccgat cgcattgctc
     6421 atcgccgtgg gcaagatgga cgctatcaac tggcaaacag catgggagcg atgctcgatg
     6481 ccgacgacgc gctaagccgc cacgaatggt tgatcgcacc gttattattg cagggcagcg
     6541 cctcgccgga tgcgcggatt ttactggcgc taccggtcga tattgatgaa ttagtacaac
     6601 gctgtccgca gctggtgcag caatctgaca ccgtggaatg ggatgacgcg caaggtacgc
     6661 tgaaagcctg gcgtcggcta caaatcggtc agttgacggt gaaagtgcag ccgctggcga
     6721 aaccgtcaga agacgagttg catcaggcga tgcttaatgg catccgtgat aaaggtttaa
     6781 gcgtgctcaa ctggacggcg gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa
     6841 agtggttgcc ggaatatgac tggccagcgg ttgatgatga aagtttgttg gcgacgctgg
     6901 aaacgtggct gctgccacat atgaccggcg tacattcact acgcggcctg aaatcactcg
     6961 acatttatca ggcactacgc ggattacttg attggggaat gcagcaacgt ctggatagtg
     7021 aattgcctgc gcattacact gtgccgacgg gaagccggat cgccattcgt tatcatgaag
     7081 ataacccgcc cgcgctggcg gtgagaatgc aggagatgtt tggtgaggcc actaatccga
     7141 cgatcgccca ggggcgcgtg ccgctggtgc tgaagttgct ttcccctgcc caaaggccgc
     7201 tgcaaatcac gcgtgatttg ggggccttct ggaaaggggc gtatcgtgag gtgcaaaagg
     7261 agatgaaagg gcgttatccc aaacacgtct ggccggacga cccggcaaac acggcaccga
     7321 cgcgacggac gaaaaagtat tcgtaaggtt ggttttctcc ctctccctgt gggagagggc
     7381 cggggtgagg gcatcagcgc gcacgttcac cctcacccta accctctccc tcaagggaga
     7441 ggggaccgat cgagcgcaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca
     7501 gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg
     7561 agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc
     7621 gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc
     7681 cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat
     7741 ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc
     7801 tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc gcggcagttt
     7861 atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa atggtgaagc
     7921 tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg
     7981 tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac agtaaagaag
     8041 ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcgaccatc gtcaatatgg
     8101 agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc atgatctcct
     8161 cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat ttgctggtgg
     8221 atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc agtctctact
     8281 ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag ggtgcgagta
     8341 cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct tactggcgta
     8401 aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc
     8461 ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct
     8521 tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc ctcgaccagc
     8581 aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg cgtaacccga
     8641 aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa cagattattg
     8701 atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg cgtggtgggg
     8761 tgatctctcc tcagccagcc tttatgcagc tggtgcgtca ggagttgcag gcaaaactgg
     8821 gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc
     8881 aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt
     8941 tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga
     9001 tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt
     9061 cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag ccgaaaatct
     9121 atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg aatggccagg
     9181 tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg
     9241 atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc
     9301 ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg catccggttc
     9361 cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcattccaga
     9421 ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta atcgcggaag
     9481 atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg gcgcaggcgg
     9541 cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt cgtcagcttg
     9601 gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag
     9661 atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta ggccgtgata
     9721 acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat cagcgttatc
     9781 tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg
     9841 gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga
     9901 ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg tcaggcaatc
     9961 cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct cagcaagagc
    10021 agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt aattaacatc
    10081 taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc atctaagata
    10141 ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca gattgcgtac
    10201 cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat cattctcgtt
    10261 tacgttatta ttcactttac atcagagata taccaatggc gcgttccaaa actgctcagc
    10321 caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc ggcatgtctg
    10381 tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc gctgcacctg
    10441 cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag tcagctaccg
    10501 gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt gtgaccgccg
    10561 aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac acgccgggtg
    10621 tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt cgcggttttg
    10681 cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag ggcaacttct
    10741 ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg cgtggcccgg
    10801 tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc agcaagcgtc
    10861 cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc ctgttccaga
    10921 ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat cgcctgaccg
    10981 gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt tatgctattg
    11041 caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt tcttacttcc
    11101 agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc gttgagccgc
    11161 tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac aacacctatt
    11221 ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac acctttactg
    11281 tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca aaacagcgtt tatggttacg
    11341 gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta gcgccagcgg
    11401 ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg caaaacttct
    11461 ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac accctgctga
    11521 ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt tacgacgact
    11581 ctgtgccact gttcaatctg tacaatccgg tgaatcccga tttcgacttc aatgccaaag
    11641 atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg ggcgtttatg
    11701 ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt tatgactggg
    11761 cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat gacaaacagt
    11821 ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct tacttcagct
    11881 atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt ttcgcaccgt
    11941 ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt ccgattgtag
    12001 ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac cctgagggtt
    12061 ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc gaagcgaaag
    12121 cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat gcggaataca
    12181 ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac atggcttcgc
    12241 tgtgggctga ctataccttc tttgacggtc cgctttcagg tctgacgctg ggcaccggtg
    12301 gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg ggaagttata
    12361 cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg ggctccaacg
    12421 tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc tttaacactt
    12481 atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt cgtttctaat
    12541 ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta tgcaggaata
    12601 cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg
    12661 cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg gtctgattgg
    12721 tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc agcctccgtc
    12781 agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca aagcgtttgc
    12841 ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga ccgtgcgtga
    12901 actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg gtgcggcaga
    12961 tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg cgcatcggct
    13021 ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc tggtggcgca
    13081 ggatagccgt tgtctgttgc tcgacgaacc gacttcagcg ctggatatcg cccaccaggt
    13141 tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg tcattgccgt
    13201 gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc tgcgcagtgg
    13261 tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat
    13321 ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg tgagttttgt
    13381 ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc gatggcgctt
    13441 tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc caatcgtatt
    13501 gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt gccttacggc
    13561 gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc ggactcagtg
    13621 attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat gaaaccgtcg
    13681 tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg tattgcgccg
    13741 ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg taaatcgctg
    13801 acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt agcgcaatac
    13861 gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg cccgttactg
    13921 ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag cttgttccag
    13981 gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa cttctggggc
    14041 agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt gctctgtttt
    14101 gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg gcaggccatg
    14161 ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta tggtgcaacg
    14221 ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa agcgtgagta
    14281 aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc gctacggcgc
    14341 tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag gccgcctggt
    14401 cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg ccgcgtctgg
    14461 cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt cagcaagtac
    14521 tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg caactgggga
    14581 ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt gctgcgctgg
    14641 caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa cggctgtcgc
    14701 cggtaacgct gattttcgcg gggttggtag tgagccttta ttgcggcgca atcaatcagt
    14761 tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc actggaacgc
    14821 tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg ggcggtgtga
    14881 tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat ggcgtggcgc
    14941 gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg gcgattgtca
    15001 tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg ttcgcgccgc
    15061 tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg gcgtcgttga
    15121 ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact cgcgtgtgga
    15181 tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta ctgtggctgc
    15241 tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt gtcgcgactg
    15301 aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg atggctgtgg
    15361 tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc
    15421 tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt gcgggcgtca
    15481 tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg gcaagcccgg
    15541 aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg tttctggtac
    15601 cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg gtgacgctgt
    15661 tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg ttactggcgg
    15721 ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca agtggtgacc
    15781 cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg accgatgctc
    15841 aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg ctgtgccgcc
    15901 gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg atggcgctga
    15961 cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc gcgacgatga
    16021 ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg atgatgggct
    16081 ttcgacggac gatgccacac atcgtaattt cggcgctggt gggtggttta ctgctggtgt
    16141 tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg gggctgctgt
    16201 cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc cgttaataga
    16261 atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc gacgtgcagc
    16321 atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc ctgcttcaaa
    16381 cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac gcttaaaacg
    16441 ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa
    16501 gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt cttctgccgc
    16561 ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt gaatgcccgg
    16621 ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt taccggaaag
    16681 cgtacccgcc tgatagaccg gacctgtcgg ggccagcgca tccattacat cacgacgacc
    16741 accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg ttaaatctgg
    16801 cactacggcg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg tcatcacttc
    16861 atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg gcaggaactc
    16921 tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa tacaggcaat
    16981 ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat aagtacaggt
    17041 taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc ctaacgtgag
    17101 tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa
    17161 tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg cggatggcgc tcatcgtcgc
    17221 ctcggtgccg gagttcacca tgcgcaccat atccatggtc gggaccagtt cggtcaccag
    17281 ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac gctcggcggc
    17341 ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg gcccccagga
    17401 accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt
    17461 ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc
    17521 gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc
    17581 ctgattcgta gaaaaagtga atggctgcta ttccatgtta ttcataacaa gttaaatact
    17641 cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt
    17701 gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg
    17761 tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag
    17821 aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg
    17881 ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gtcgggctgg
    17941 ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg
    18001 tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt tcggcggtgc
    18061 tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg ggatcgggga
    18121 tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg cgtgtattgc
    18181 cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggggcta gggcgcgaag
    18241 gaccaaccgt gcagatcggc ggcaatatca gtcgcatggt gcttgatatt ttccgcctga
    18301 aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg
    18361 cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc
    18421 gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg accattatgt
    18481 accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc
    18541 ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt
    18601 ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta
    18661 ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg gggtttgtgg
    18721 caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca
    18781 gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta ctctgcttct
    18841 cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa
    18901 ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag gcggggacgt
    18961 ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga
    19021 tcattctggt tctggagatg accgataact accagctcat tttgccaatg attattaccg
    19081 gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac tcggcgattc
    19141 ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag gccgcatcag
    19201 ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt
    19261 agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc
    19321 cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata
    19381 acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg
    19441 gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg
    19501 gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt gactacaccg
    19561 aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt
    19621 gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta tccggcacat
    19681 cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg
    19741 aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag
    19801 tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt
    19861 tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg accagtacat cacgaatgat
    19921 cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac cggcttccgc
    19981 attaaaggct ttattcacgc taatgccgac aaacaccgcc agaccaacgg cgtccagcac
    20041 cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct
    20101 ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc
    20161 cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca gtaccagaac
    20221 accaaaaggg tccatacgca attttccggc taacaaaacg ccgtagatgg caaataccgc
    20281 tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga gcacctgaaa
    20341 aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct gcgaggataa
    20401 tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct
    20461 gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatag
    20521 cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt
    20581 ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc gactcttttc
    20641 cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag
    20701 catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct tgttctgcct
    20761 tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt tgttcaatgc
    20821 ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct
    20881 cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca
    20941 gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg
    21001 aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg
    21061 gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga aaagacaggg
    21121 cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag tgactcaacc
    21181 atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt
    21241 tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag
    21301 acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg
    21361 attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt
    21421 acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca
    21481 gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca
    21541 tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt cagcgttggt
    21601 gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc
    21661 agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg
    21721 gttccattca gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt
    21781 tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc
    21841 atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc ttacggggaa
    21901 gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg
    21961 gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt
    22021 cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac
    22081 ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga
    22141 agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga aagagcttaa
    22201 attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca ttgttgagat
    22261 gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat
    22321 aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga
    22381 cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc ttaacgagct
    22441 gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct
    22501 ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta ttttaaaata
    22561 tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa
    22621 gccgggttat tatctttctg aagaagccta tattgcccgc ttgcgtaaag aacttaattt
    22681 ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta
    22741 ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg agcagcttta
    22801 tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt cgctggtggt
    22861 tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt
    22921 ttttatgtat ttacgggtaa acaccctaaa taaactggta ccctacgcgg cacaacgatt
    22981 tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat tggaagatgc
    23041 cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac atgtgtttag
    23101 ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg gattattaga
    23161 gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg tagaaaaaga
    23221 acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga cgcgccatcg
    23281 gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt ttccgctatg
    23341 ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga ccgacctcta
    23401 tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccagtct tttgtaaaga
    23461 cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg ctataaaacg
    23521 aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat acatgaaaaa
    23581 aaacacatta gcactgagtg cactggctct gagtttaggt ttggcgttat ctccgctctc
    23641 tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa gccttgcacc
    23701 gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta gcacaaccgt
    23761 taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt ctccgttctg
    23821 ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg gcggtaatgg
    23881 cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg atgccgataa
    23941 aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta aagtccagct
    24001 gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc
    24061 gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg attctgatgc
    24121 actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg gcgagacggt
    24181 aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa actacgaaaa
    24241 cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct
    24301 gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg
    24361 tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga tggtggaata
    24421 cggccaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt ccgaactggc
    24481 gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc tgcctaattc
    24541 ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga acggtaagcc
    24601 gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg gcagcaaact
    24661 gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac tgcagcagag
    24721 cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg ctgagatgag
    24781 caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg aagacgggca ctccggctgc
    24841 gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg cagtgaaaaa
    24901 catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac tgaacattca
    24961 gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc cttcctgaat
    25021 acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac atcttcatca
    25081 tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa gccgtgcata
    25141 acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat atcgtggcac
    25201 gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg cgaattatcg
    25261 gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg gtactttcac
    25321 agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt gtgcggcagg
    25381 ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg
    25441 aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa atgatgctgg
    25501 aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa gaactggtga
    25561 tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc aacggctggg
    25621 gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg
    25681 cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta cgcccgagcg
    25741 taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac cggcgttgaa
    25801 ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac tgattacccg
    25861 catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt ttaccggtcc
    25921 tggcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg
    25981 gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag
    26041 tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaagcgat
    26101 ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca atgtgcaacc
    26161 gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc ggcttaatcg
    26221 tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt
    26281 ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg gcgctggctc
    26341 attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat tcgggattat
    26401 ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt tacgactgac
    26461 tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct gcggtcacgc
    26521 tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat ttcctggact
    26581 tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg cggatattta
    26641 aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat tttcagaata
    26701 tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt ggtaataccc
    26761 agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa ttgtgcttat
    26821 tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct ttttcttcca
    26881 gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg atattccatg
    26941 ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga aacaagatga
    27001 aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg agccagaaag
    27061 catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac gcagcagttc
    27121 gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa ccgcacagta
    27181 gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag accccgccgg
    27241 aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc tctgaccaat
    27301 gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa ccacttcaga
    27361 gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca gcggctccag
    27421 cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct gcgcacaaga
    27481 accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc cgatgttaac
    27541 gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta ccgccgctgg
    27601 tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg cgaatttcat
    27661 cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat cattaatacg
    27721 gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac cgtcaatttt
    27781 ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta ccgcttcgcg
    27841 ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag cggtttcaat
    27901 aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac atcacccttt
    27961 atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca actcgttatt
    28021 aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct cgccaatggt
    28081 tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc cgactcgcgc
    28141 cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt cggtatgggt
    28201 cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg gttggcgacg
    28261 gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa accgaataac
    28321 ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa aggtatccat
    28381 cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat tgcggcggtc
    28441 taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga taaaaatctc
    28501 ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta aatcatgctg
    28561 taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa aatagttagc
    28621 acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca tgcgcagtag
    28681 tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg tgttttgcat
    28741 tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac tttgcttcca
    28801 gctattccac agtgtttcgt tggtggcgca aatgtcagcc actgtcaggc ataccagata
    28861 gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt cagggtcctg
    28921 aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca gccaggcaac
    28981 cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta catcctgagc
    29041 accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca gcgcggcgat
    29101 gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg gatggcgctg
    29161 gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag tatgttcatc
    29221 caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg accattgcgg
    29281 catatacgcg ccgagcacgc tatggcgatg cattggcaat agtccgcgcc gcaccgctcc
    29341 ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac acagcggttg
    29401 ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa tgccggtgat
    29461 cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct ggcgcataaa
    29521 tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat cgtcgagtgg
    29581 acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt gcagcagcat
    29641 ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca tccgctcgac
    29701 cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt ggcgatcgaa
    29761 taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa tacgccacag
    29821 tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc caaacccgac
    29881 catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca gagtgtgaat
    29941 atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc tggtgccatg
    30001 gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact tgtcggaagg
    30061 ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa catcgccaat
    30121 taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc cttccaacat
    30181 gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc agagcagcgt
    30241 taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc ttaaaatcag
    30301 taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga cggcaaccaa
    30361 tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca ggagctggtc
    30421 tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt caaaggcatc
    30481 acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg ttaattcatc
    30541 acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag cggtgtttgc
    30601 gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta tcgcattaaa
    30661 aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc gggatggtgt
    30721 catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata gtatgctcat
    30781 attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc atggtgcgga
    30841 tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc ggtttcagca
    30901 cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg aagccgcgac
    30961 caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg acaaatttct
    31021 gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt agcgccaggt
    31081 acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc ttaccgacga
    31141 taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca tcaatgttaa
    31201 cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac accacttcat
    31261 taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca gaaaccgcgt
    31321 gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg ctgacgcccg
    31381 gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca gcgacgcgca
    31441 ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc catcagcgtc
    31501 ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg gcaaattgag
    31561 aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga aaattcgacg
    31621 tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg tggtataaag
    31681 cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac aatctcactt
    31741 tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg tcggtaatat
    31801 gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg gcaactgttt
    31861 ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt tactggaacc
    31921 cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc aaccttgaga
    31981 aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt tctcgcaaag
    32041 gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa gacgctgctc
    32101 tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg actaactgga
    32161 aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct caggacggta
    32221 ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg gagaaactgg
    32281 aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg tttgtaatcg
    32341 atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt ccggtatttg
    32401 ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg ggtaacgacg
    32461 acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta cgtgaaggcc
    32521 gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag taataaggct
    32581 tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag aattcccggg
    32641 agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg cggcgcgaaa
    32701 tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg ggcctgcata
    32761 tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc acatctccga
    32821 ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg
    32881 cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca tcgagctggc
    32941 aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag gcaacgttgc
    33001 tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc tggaagttaa
    33061 ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag acaaagttct
    33121 ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga
    33181 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc
    33241 gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc
    33301 tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg ctgcaagcaa
    33361 gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag aataccaggt
    33421 acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga aaatggttga
    33481 aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga
    33541 accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat
    33601 ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc
    33661 tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg cttctttttg
    33721 tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg
    33781 tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat ggctaccaat
    33841 gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct gcagggcact
    33901 gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat caaagaactg
    33961 gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct
    34021 ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat gctggcgacc
    34081 gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg
    34141 atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga agctatcagc
    34201 ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc gttctttacc
    34261 accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca
    34321 accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc aaccatgtac
    34381 gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga cctggcggcc
    34441 ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa caaaccgggt
    34501 gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga ataattcccg
    34561 tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag tctggttata
    34621 aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg agcgtacaaa
    34681 agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat agcctaataa
    34741 tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact tccactgtgt
    34801 gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat tagcgatatc
    34861 agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac ccaaatcagc
    34921 aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt ggaatattac
    34981 ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc ccgtactctg
    35041 aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat tatggcctcc
    35101 gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct gccgccgctg
    35161 acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga acaagcgcgt
    35221 gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact gttgaaagat
    35281 aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa actgactgat
    35341 gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact gatgcagttc
    35401 tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc tggcggcgtt
    35461 ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca ttctgggctc
    35521 gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg aacacttccg
    35581 cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt gcctggaatt
    35641 ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta aaacgatgct
    35701 acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt gcgatatggc
    35761 agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg ggctgttacc
    35821 tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag aatcactggt
    35881 tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat tgttaccggt
    35941 cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc ataatctggg
    36001 atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt ctggtggccc
    36061 tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag cttgccgtca
    36121 tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga tgaacaaagg
    36181 tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga tggaagtgct
    36241 gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca gtgttctggc
    36301 gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat ggccgaatcg
    36361 cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga catttgccgc
    36421 accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg aacaaggcca
    36481 ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt ttcttgcgca
    36541 acaaatccgc tttacggata tcgctgcgtt gaatttatcc gtactggaaa aaatggatat
    36601 gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga gtgaagtcgc
    36661 cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag agtcgcgcta
    36721 tttgttagcg tagggcttca gtgatatagt ctgcgccatc tgatcgtaag tagttggctt
    36781 tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct tcagtattcc
    36841 tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc tactcaacca
    36901 cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat ggacggcaat
    36961 ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc cggggcaaaa
    37021 tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaggcgtt aacgctgtat
    37081 gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg
    37141 tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt gcgtctgcgt
    37201 attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg taaatctgaa
    37261 gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta cggtggacgt
    37321 tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa
    37381 ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga actggcccct
    37441 gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt gctttggcaa
    37501 attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga tgaacaagac
    37561 tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg caccgagccc
    37621 ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat atctgctttt
    37681 gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg gttcgccatt
    37741 gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag cggttttacc
    37801 actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc gctgatgctt
    37861 tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat ctcactttgg
    37921 gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc aggttccgca
    37981 gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac cattgttccc
    38041 ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca ttacagtggc
    38101 gcaatatggc tgctctatgt catgatcctg gtatggggcg ctgactccgg cgcatatatg
    38161 tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg taaaacctgg
    38221 caaggcttta taggtggact cgctactgca gcggtaatct catggggtta tggcatgtgg
    38281 gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc agcgttagcc
    38341 tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat taaggacagc
    38401 ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct gacggctgcg
    38461 gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg gaaggtaata
    38521 tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta cttatcaccg
    38581 tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct
    38641 caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc gaatatgtta
    38701 tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca gaaccggtcg
    38761 ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga gcggcgatta
    38821 ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg ctggttttta
    38881 ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat tcgatagctg
    38941 cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc gaaacgcctg
    39001 attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc accaccatta
    39061 cagtagcgcc atttgacagc gaccaacggc gggatgtaaa gctcgattta cgtcactggg
    39121 cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct cgtgggccgc
    39181 aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag gcaggtttgc
    39241 aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg
    39301 tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa aggcagggga
    39361 gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa gcgattggtt
    39421 ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt gtacgccagt
    39481 atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg atgaagctga
    39541 cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc
    39601 cgatctctat cgccaagggg gctgggatga cagcggaact cggggtagtt tattacctgc
    39661 cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg ttgcccgtac
    39721 ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga ccggtatccg
    39781 agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg ttaatggggc
    39841 ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac gcataataac
    39901 gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg
    39961 tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg tcgccgttgg
    40021 tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg aagatatcag
    40081 taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg tccttcgtga
    40141 tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca ttactttctc
    40201 cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac ctcgaggctt ctggtgtgcg
    40261 tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc tggaagactt
    40321 ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc cgctgccgcg
    40381 caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg tcagctgaaa tccagcaaat
    40441 taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt tccaactgcg
    40501 tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac agaaactggc
    40561 gggcgatctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc gtttcaacat
    40621 cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca cggtgaacat
    40681 caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc ttgccgggca
    40741 ctccgctgaa attgagcaac tgactaagat cgagccgggc gagctgtata acggcactaa
    40801 agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt atgcctatcc
    40861 gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat tacgcgtgaa
    40921 cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta acgatacctc
    40981 gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt tggggagcga
    41041 tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac
    41101 cgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca aggtgaaaga
    41161 acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg gcgtgagctt
    41221 ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg gtatcaacgg
    41281 gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt acttcaccgt
    41341 agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag atgacgccga
    41401 cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct tcccgattaa
    41461 cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt ccaacatgca
    41521 gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga gcacctctga
    41581 tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga cctataacaa
    41641 gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac
    41701 cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga cttatgtgcc
    41761 gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt atggtgatgg
    41821 tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt ccagcaccgt
    41881 gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc atcaggccag
    41941 taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga aagacctgtg
    42001 taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg agttcatcac
    42061 cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat tcttctggga
    42121 tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat atccggacta
    42181 tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga tgtccccatt
    42241 ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag acaaggcaga
    42301 acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg
    42361 tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca aagaactgca
    42421 ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta ttagctgcag
    42481 gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca atcgtcaata
    42541 tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg
    42601 agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag gctaaaatga
    42661 aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa gacgtgatgg
    42721 ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt
    42781 ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa tccgttgcca
    42841 acagccagga tatcgatctg gttgttgatg caaacgccgt tgcttacaac agcagcgatg
    42901 taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct tcaattcgac
    42961 tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat atcgtcatca
    43021 ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg gttaacccaa
    43081 aataccgtga gcatttaggc ttgtgccagg cgtccgcggt tgtcatgacc caggacgatc
    43141 ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact tacgcgcgca
    43201 tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga
    43261 tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg gtgattgagt
    43321 ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca
    43381 gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat gagatccaga
    43441 tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc tttggttatg
    43501 ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt attggcgatc
    43561 gcgtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac actgttattg
    43621 gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg attggcgaca
    43681 atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt cgttactgca
    43741 tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa gtgacggtta
    43801 cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc tcaggcattc
    43861 cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca
    43921 tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa cgttccatct
    43981 tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc cgtgttatta
    44041 ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc atactctgca
    44101 gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt
    44161 gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc
    44221 attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga ttctggaagc
    44281 aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga
    44341 gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga
    44401 tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg
    44461 ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg
    44521 ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga
    44581 gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg gaccccatgt
    44641 cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc atactaaaat
    44701 tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc aggatttgaa
    44761 atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt
    44821 caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca gcgacaactt
    44881 actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc
    44941 caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat
    45001 gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg gctgctccgg
    45061 tgtggcgcag gacgtccctc cttatgttat tgcgcagggt aaccacgcaa cgccgttcgg
    45121 tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta ccgctatccg
    45181 caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga aaccggaaat
    45241 tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct ttgcacgctc
    45301 aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc cctggtcgcc
    45361 ggagaaacct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg
    45421 cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg ctgcgaagcc
    45481 tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg tcgtctgcgt
    45541 cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa gccagatgtt
    45601 tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct caaaaagcag
    45661 ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca gaaacgcgtt
    45721 ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt
    45781 tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga tgccatgccg
    45841 ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga tgcccactgt
    45901 ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc cgatttcttg
    45961 aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt gccgctggtg
    46021 aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc agacctgtcg
    46081 gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc ggcactactg
    46141 gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt ggtgggatat
    46201 cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga ttatgtctcg
    46261 ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga agagtgtgag
    46321 ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac cagccacgcg
    46381 atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga tgagcaggcg
    46441 gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac acgcagctgg
    46501 ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg
    46561 tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag ctgagcgaaa
    46621 aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg agtctgggcc
    46681 gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg ctggcgatgc
    46741 agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat ggtaaccgct
    46801 gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc gtaccagaaa
    46861 tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata
    46921 ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct tttcatctgg
    46981 aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg cctgtcaaac
    47041 gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg aatctgaaga
    47101 tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg atcgatggct
    47161 tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga
    47221 tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga catggcgcag
    47281 ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg ggtgatgagt
    47341 taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg gcgttgctga
    47401 tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat cgcgactggc
    47461 ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc gacgtcggac
    47521 gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt tatgaagaac
    47581 acttcccgga tcgctatttt ctcgagctga tccgcaccgg caggccggat gaagaaagct
    47641 atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg gcgactaacg
    47701 atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc gcgatccacg
    47761 acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc
    47821 gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt gccaacaccg
    47881 ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc ctgccgcagt
    47941 tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa gaaggcctgg
    48001 aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc cgcccggaat
    48061 atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggcttc ccgggctact
    48121 tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg gtagggccag
    48181 gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc gacctcgatc
    48241 cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg
    48301 acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac gtggcggaca
    48361 tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg gcgaaagcgg
    48421 tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac cgtatctcga
    48481 aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc
    48541 tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg gcgcgcaaac
    48601 tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc gcgccgacca
    48661 aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg gtcacccagt
    48721 ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt ggtttgcgta
    48781 cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg
    48841 agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc gacatgctgc
    48901 aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag gacctgatca
    48961 agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg ttccgccctg
    49021 gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt cgtgaagaga
    49081 tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg gagccaacct
    49141 acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt tctggttata
    49201 ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg gaagagatgg
    49261 ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac gccgaactgg
    49321 cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac aaatcgcact
    49381 ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac tatccggcgg
    49441 agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg gtgggcctgg
    49501 tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac tccggtcttt
    49561 accatttcca cgtcaacgac gatggcgaaa tcgtgtatgg tattggcgcc atcaaagggg
    49621 taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc tacttccgcg
    49681 aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg gtgctggaaa
    49741 aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg ctgatgaact
    49801 cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct atcggtcagg
    49861 ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc tacgccagct
    49921 gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta ggcctgtacc
    49981 tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc ggaggcgtaa
    50041 ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg gggctcgttg
    50101 ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc acgctggatg
    50161 accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa taccagcaat
    50221 tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat gacttcagcg
    50281 gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg gaaaaatatg
    50341 ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt ttaaaccgac
    50401 tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc tactatcaga
    50461 gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc
    50521 gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg gagtttgact
    50581 aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt gcagagctgg
    50641 aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg gatattaaca
    50701 tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt aaaatcttcg
    50761 ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt ccttataccc
    50821 tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac cgtgcgtatg
    50881 cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg gtgatgatca
    50941 ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag
    51001 cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc tttaagatgc
    51061 ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca gaagagcgtg
    51121 gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc gtaccggtag
    51181 tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc gtgggcgata
    51241 aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt tgtgcgtcca
    51301 ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt atcatcgctc
    51361 cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg ggcggcgctc
    51421 accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg gatctggccg
    51481 atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct
    51541 acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc ccttttttat
    51601 cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac acatgaacat
    51661 cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca aagaactgga
    51721 gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg ttgatttgct
    51781 gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg atgagtacag
    51841 tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt atgccttcat
    51901 caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc tctggttttt
    51961 tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt acaccaacga
    52021 atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg tcaaagagcg
    52081 gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa aaagcccggt
    52141 tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg tctctatttc
    52201 ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag cggaagagta
    52261 catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggaa tatcgacgtc
    52321 gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga tcgaccgcaa
    52381 ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag tctggctgaa
    52441 accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat ttactcgcga
    52501 cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc atgcggtgat
    52561 caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac agacgctgga
    52621 tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc atccaatcta
    52681 ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct tcgaaacgca
    52741 atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca ttaaaggcga
    52801 gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct cgcccagtta
    52861 tcccattgtt gcttcggtta agacggcggc ggcgatgctg cgtggtaatc cgggcaaacg
    52921 gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc agcggctgcg
    52981 ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga
    53041 atgcaggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg atgccgatca
    53101 tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg aacagggcaa
    53161 tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg aacgtgggat
    53221 cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg gcatcgataa
    53281 aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt acgatctcaa
    53341 cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt tctaccgcaa
    53401 tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac acgatctttc
    53461 cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc cacatcaggc
    53521 atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac tggtcggtag
    53581 agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga tgccaggaga
    53641 aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt gttccgtagg
    53701 gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg aagacggcgt
    53761 ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt ccgggcgagt
    53821 aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt tcaccatatt
    53881 gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat tcttggtttc
    53941 acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt
    54001 aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg ccccagccga
    54061 ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga tgcggcagtg
    54121 gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa
    54181 aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta tgagcagtaa
    54241 ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt aaaatcgcac
    54301 catcatgaca ctcacgctca atagacaact tctcacctca cgccagattc tggtggcctt
    54361 tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc ggacggaaaa
    54421 tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca atgccgatgc
    54481 ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg tggtcgaacg
    54541 cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg cacgttatca
    54601 ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac atctcgacga
    54661 tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg ggctttcggc
    54721 tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc ttgcccgcac
    54781 gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg aagacgaaag
    54841 taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc cgttattgca
    54901 gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt gtgctgaaca
    54961 agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa cgtcgcaggg
    55021 gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg cgattattcg
    55081 ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg tgcgcatctg
    55141 gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg gcgcctttga
    55201 aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc aaagcgaaac
    55261 cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc tgggaaccgt
    55321 acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg tcagcgtgcg
    55381 tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta agctgaagaa
    55441 aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac tgctgtttta
    55501 tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg tggctgaagg
    55561 tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag ccggataaga
    55621 cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc accgtaccga
    55681 tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt tcgccagcgg
    55741 cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct tttgcctgca
    55801 atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag gcgagctcaa
    55861 gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac ataatcgctc
    55921 accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga aagggcgtcg
    55981 tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg aacgtatcct
    56041 aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata tttttccaca
    56101 tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta cgaagataaa
    56161 ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc cgcaactgca
    56221 cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg cttaaagcga
    56281 caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt ctcgatgcct
    56341 ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg cgcttgctgg
    56401 cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt gccgatgatg
    56461 agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt gagctggggc
    56521 tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg gcgctgttta
    56581 cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa tgtgtgcagt
    56641 ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta agcgcctttg
    56701 ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg ttatcggatg
    56761 ataagaataa tctggaagtt aacttaaccg cctggcaaca accttcatga ttgtgatttc
    56821 ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg cgcagggcgc
    56881 tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg acattcgggc
    56941 gtccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc atttgatcag
    57001 cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac tgaaccgcga
    57061 agccgctctg gcccggctgg tggcagtgat taaagattta acaaaagaac aaaaagcccg
    57121 acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga aagcacaaaa
    57181 atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa aagaaggaat
    57241 ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt actctgatgg
    57301 gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc gaactgaaac
    57361 cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga atcgaaatct
    57421 ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc ggtgctcgtg
    57481 aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac aagctggtat
    57541 taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg ctggagatgc
    57601 tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa ccggcacaat
    57661 ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg gctgatgcgg
    57721 cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac gcagagctgg
    57781 agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg tcagtagaag
    57841 gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta ttagcgcccg
    57901 atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag taaacctgct
    57961 taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc tacagcatat
    58021 aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta cgccgcatcc
    58081 ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc gacccgcctt
    58141 gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg gcttaaacgc
    58201 agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc ccgattgcgc
    58261 cagtgtaagt ggatgcccgg atttggcaaa tgcatcagtg acaaattccg aacaaaacca
    58321 cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt cgctgacgca
    58381 ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag tcaccataaa
    58441 gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt tattggcaaa
    58501 cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga cgaaaagctt
    58561 atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc ctgtcgcttc
    58621 tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag tgctgaagac
    58681 gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc cgggtttgag
    58741 atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat gctggaattt
    58801 gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg gctggctgat
    58861 atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc agtacgcctt
    58921 tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag gaccctttct
    58981 ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc ttaattaact
    59041 gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg ttacggtcgc
    59101 ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc acgcccggac
    59161 gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg ctgtacagtt
    59221 tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga atcgccacct
    59281 ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc tgctcaatcg
    59341 ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg atttggttac
    59401 ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta cccagctgga
    59461 agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca tccgggcttg
    59521 gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca tcgcgatccc
    59581 agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa tcactcatcg
    59641 ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc agtgaacccg
    59701 gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa
    59761 cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc accagcagcg
    59821 caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta accgttttct
    59881 caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc ggcgtatcaa
    59941 ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata gcttctgcca
    60001 gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct tcaccgctct
    60061 gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg tcggcctgta
    60121 cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca tacattgcat
    60181 cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct ttcatcagga
    60241 attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac ttggtctgga
    60301 tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga atcaggtcag
    60361 tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg tcaacaaaac
    60421 gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa tcggctggct
    60481 gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca cgcacgatgt
    60541 tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac ccggaggcca
    60601 gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg gcgtcggcag
    60661 gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta cggttccagt
    60721 tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta gtttaccagt
    60781 gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg caaagacttc
    60841 aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga cggcataggt
    60901 tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca gtacttcgcg
    60961 gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct tttcgacctc
    61021 tgcggtaaaa ctcaccgcca tctctgcagc tggcgtactt tgcgcatagc tggcactggc
    61081 atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg gcgtaccatc
    61141 gaccagatcc agactgccga gcttcagaat cacgctgcct ttatggcaaa caacctcttt
    61201 cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg ttgcgaaaac
    61261 ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc agccgccttc
    61321 catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca ggccgcgaac
    61381 ggcgtcggcc tggttgtagg aagctatgag atgcagttcg ccgttggcgc tttttaccag
    61441 acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa cgcctatttg
    61501 ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat acatacagcc
    61561 tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa tacagcattg
    61621 gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat gctcggcgga
    61681 gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc gcggaacggt
    61741 ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg cggcgctttc
    61801 ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc gacaggggat
    61861 ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg taaagcacgc
    61921 atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct tgtaaaaatg
    61981 acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca gcctaaaaat
    62041 taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta aacttcatca
    62101 gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt
    62161 acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc tttcgccgga
    62221 gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat ttgcgcgtcg
    62281 tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt ttttgggttc
    62341 tcaacaacat caagaacggt cggcagcagg ccaacgccat ctttcagttt gatcaagccc
    62401 actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg cacggcaacc
    62461 tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc agcaatcgga
    62521 taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag ttgctgatca
    62581 aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt
    62641 ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt gtctttcgca
    62701 actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc gactttaatg
    62761 tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc aatcagggct
    62821 cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt caattattta
    62881 tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc tgcaaactga
    62941 attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt cgcgttgtag
    63001 ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac tgcaccaccc
    63061 atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac cagacccggc
    63121 aacgcttccg gtaacagcac tttacagacg atctgcattg gcgtggcacc cattgcgcgg
    63181 gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat acgggcaata
    63241 aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat cgatgtaccg
    63301 acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg gatggaacgg
    63361 aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat aatttgcccc
    63421 ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc aaaaaatccg
    63481 gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca catcatcggc
    63541 tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg caatggcggc
    63601 ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt taacgccacc
    63661 ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac gcgcggtttc
    63721 agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca tcggcacgca
    63781 gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat ccagatgcag
    63841 ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca cttcacttac
    63901 cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac aaatacgctt
    63961 cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc ggttaatgtc
    64021 tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg tggcttcatc
    64081 acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac gttgtttctg
    64141 cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa ccaatgacag
    64201 caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct ccagcggcag
    64261 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
    64321 aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt cctggccatc
    64381 gaccagcacg ctaccctcgg ttgggcgctc cagcaagttt acacaacgta taagcgtact
    64441 cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
    64501 gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg tgatattcga
    64561 aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc tcgaatgcct
    64621 gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
    64681 ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg tcccatgcct
    64741 tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg accgtgatgg
    64801 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
    64861 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg tagtaaccaa
    64921 ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc tgaccgagtg
    64981 gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt attgcccgca
    65041 tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca aaccacatcc
    65101 ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
    65161 gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa caaaagtgct
    65221 ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt gggtgttaaa
    65281 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc aatgattaaa
    65341 agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc gactccctat
    65401 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    65461 aagagaaaaa atcctgaaat tcagggttga ctctgaaata ggaaagtgtg atatacgcca
    65521 cctcgcgaca gtgagctgaa agctgcgtcg caactgctct ttaacaattt atcagacaat
    65581 ctgtgtgggc actcgaagat acggattctt aacgtcgcaa gacgataaat gaataccaag
    65641 tctcaagagt gaacacgtaa ttcattacga agtttaattc tttgagcatc aaacttttaa
    65701 attgaagagt ttgatcatgg ctcagattga acgctggcgg caggcctaac acatgcaagt
    65761 cgaacggtaa caggaagcgc ttgctgcttc gctgacgagt ggcggacggg tgagtaatgt
    65821 ctgggaaact gcctgatgga gggggataac tactggaaac ggtagctaat accgcataac
    65881 gtcgcaagac caaagagggg gaccttcggg cctcttgcca tcggatgtgc ccagatggga
    65941 ttagcttgtt ggtggggtaa cggctcacc
//
LOCUS       DAZPDE010000002        63823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPDE010000002 DAZPDE010000000
VERSION     DAZPDE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.002"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHU0J_000062"
     CDS             <1..795
                     /locus_tag="ACHU0J_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1214944.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHU0J_000063"
     CDS             806..1897
                     /locus_tag="ACHU0J_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1214945.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHU0J_000064"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHU0J_000064"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1214946.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHU0J_000065"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHU0J_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1214947.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHU0J_000066"
     CDS             4859..5254
                     /locus_tag="ACHU0J_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1214948.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHU0J_000067"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHU0J_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1214949.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHU0J_000068"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHU0J_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1214950.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHU0J_000069"
     CDS             complement(6563..7603)
                     /locus_tag="ACHU0J_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1214951.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHU0J_000070"
     CDS             complement(7676..8311)
                     /locus_tag="ACHU0J_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1214952.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHU0J_000071"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHU0J_000071"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1214953.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHU0J_000072"
     CDS             complement(8937..9380)
                     /locus_tag="ACHU0J_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1214954.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHU0J_000073"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHU0J_000073"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1214955.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHU0J_000074"
     CDS             complement(9720..10223)
                     /locus_tag="ACHU0J_000074"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1214956.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHU0J_000075"
     CDS             complement(10217..10741)
                     /locus_tag="ACHU0J_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1214957.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHU0J_000076"
     CDS             10950..11945
                     /locus_tag="ACHU0J_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1214958.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHU0J_000077"
     CDS             11954..12832
                     /locus_tag="ACHU0J_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1214959.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHU0J_000078"
     CDS             12913..13920
                     /locus_tag="ACHU0J_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1214960.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHU0J_000079"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHU0J_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1214961.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHU0J_000080"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHU0J_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1214962.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHU0J_000081"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHU0J_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1214963.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHU0J_000082"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHU0J_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1214964.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHU0J_000083"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHU0J_000083"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1214965.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHU0J_000084"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHU0J_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1214966.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHU0J_000085"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHU0J_000085"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1214967.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHU0J_000086"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHU0J_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1214968.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHU0J_000087"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHU0J_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1214969.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHU0J_000088"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHU0J_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1214970.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHU0J_000089"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHU0J_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1214971.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHU0J_000090"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHU0J_000090"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHU0J_000091"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHU0J_000091"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1214972.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHU0J_000092"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHU0J_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHU0J_000093"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHU0J_000093"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHU0J_000094"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHU0J_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1214973.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHU0J_000095"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHU0J_000095"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1214974.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHU0J_000096"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHU0J_000096"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1214975.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHU0J_000097"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHU0J_000097"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1214976.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHU0J_000098"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHU0J_000098"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1214977.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHU0J_000099"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHU0J_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1214978.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHU0J_000100"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHU0J_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1214979.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHU0J_000101"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHU0J_000101"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1214980.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHU0J_000102"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHU0J_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHU0J_000103"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHU0J_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1214981.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHU0J_000104"
     CDS             complement(41212..42177)
                     /locus_tag="ACHU0J_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1214982.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHU0J_000105"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHU0J_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1214983.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHU0J_000106"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHU0J_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1214984.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHU0J_000107"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHU0J_000107"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1214985.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHU0J_000108"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHU0J_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1214986.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHU0J_000109"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHU0J_000109"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1214987.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHU0J_000110"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHU0J_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1214988.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHU0J_000111"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHU0J_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1214989.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHU0J_000112"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHU0J_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1214990.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHU0J_000113"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHU0J_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1214991.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHU0J_000114"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHU0J_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1214992.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHU0J_000115"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHU0J_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1214993.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHU0J_000116"
     CDS             49715..50680
                     /locus_tag="ACHU0J_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1214994.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHU0J_000117"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHU0J_000117"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1214995.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHU0J_000118"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHU0J_000118"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1214996.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHU0J_000119"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHU0J_000119"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1214997.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHU0J_000120"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHU0J_000120"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1214998.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHU0J_000121"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHU0J_000121"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1214999.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHU0J_000122"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHU0J_000122"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1215000.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHU0J_000123"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHU0J_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1215001.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHU0J_000124"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHU0J_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1215002.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHU0J_000125"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHU0J_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1215003.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHU0J_000126"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHU0J_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1215004.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHU0J_000127"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHU0J_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1215005.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHU0J_000128"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHU0J_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHU0J_000129"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHU0J_000129"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1215006.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHU0J_000130"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHU0J_000130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1215007.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHU0J_000131"
     CDS             complement(62547..63476)
                     /locus_tag="ACHU0J_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1215008.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
BASE COUNT        15705 a        17308 c        15627 g        15183 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aag
//
LOCUS       DAZPDE010000003        58574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPDE010000003 DAZPDE010000000
VERSION     DAZPDE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.003"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHU0J_000132"
     CDS             complement(<1..1101)
                     /locus_tag="ACHU0J_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1215009.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHU0J_000133"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHU0J_000133"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1215010.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHU0J_000134"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHU0J_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1215011.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHU0J_000135"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHU0J_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1215012.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHU0J_000136"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHU0J_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1215013.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHU0J_000137"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHU0J_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1215014.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHU0J_000138"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHU0J_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1215015.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHU0J_000139"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHU0J_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR1215016.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHU0J_000140"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHU0J_000140"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1215017.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHU0J_000141"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHU0J_000141"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1215018.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHU0J_000142"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHU0J_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1215019.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHU0J_000143"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHU0J_000143"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1215020.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHU0J_000144"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHU0J_000144"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1215021.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHU0J_000145"
     CDS             11851..12015
                     /locus_tag="ACHU0J_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215022.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHU0J_000146"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHU0J_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1215023.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHU0J_000147"
     CDS             13524..13769
                     /locus_tag="ACHU0J_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1215024.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHU0J_000148"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHU0J_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1215025.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHU0J_000149"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHU0J_000149"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1215026.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHU0J_000150"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHU0J_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1215027.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHU0J_000151"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHU0J_000151"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1215028.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHU0J_000152"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHU0J_000152"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1215029.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHU0J_000153"
     CDS             19432..19785
                     /locus_tag="ACHU0J_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1215030.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHU0J_000154"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHU0J_000154"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1215031.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHU0J_000155"
     CDS             21085..22212
                     /locus_tag="ACHU0J_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR1215032.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHU0J_000156"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHU0J_000156"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1215033.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACHU0J_000157"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACHU0J_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHU0J_000158"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACHU0J_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHU0J_000159"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACHU0J_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1215034.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHU0J_000160"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACHU0J_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1215035.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACHU0J_000161"
     CDS             26834..27583
                     /locus_tag="ACHU0J_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1215036.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACHU0J_000162"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACHU0J_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1215037.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHU0J_000163"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACHU0J_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1215038.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHU0J_000164"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACHU0J_000164"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1215039.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHU0J_000165"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACHU0J_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1215040.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHU0J_000166"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACHU0J_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1215041.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHU0J_000167"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACHU0J_000167"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1215042.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACHU0J_000168"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACHU0J_000168"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1215043.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHU0J_000169"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACHU0J_000169"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1215044.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMFIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHU0J_000170"
     CDS             complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACHU0J_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1215045.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHU0J_000171"
     CDS             complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACHU0J_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1215046.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36031)
                     /locus_tag="ACHU0J_000172"
     CDS             complement(35891..36031)
                     /locus_tag="ACHU0J_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1215047.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHU0J_000173"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACHU0J_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1215048.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="ACHU0J_000174"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="ACHU0J_000174"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1215049.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="ACHU0J_000175"
     CDS             40511..40762
                     /locus_tag="ACHU0J_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1215050.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="ACHU0J_000176"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="ACHU0J_000176"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1215051.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="ACHU0J_000177"
     CDS             42067..42825
                     /locus_tag="ACHU0J_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1215052.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="ACHU0J_000178"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="ACHU0J_000178"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1215053.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="ACHU0J_000179"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="ACHU0J_000179"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1215054.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="ACHU0J_000180"
     CDS             complement(44425..45045)
                     /locus_tag="ACHU0J_000180"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1215055.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="rnm"
                     /locus_tag="ACHU0J_000181"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(45042..45923)
                     /gene="rnm"
                     /locus_tag="ACHU0J_000181"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1215056.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="ACHU0J_000182"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="ACHU0J_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1215057.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="ACHU0J_000183"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="ACHU0J_000183"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1215058.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="ACHU0J_000184"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="ACHU0J_000184"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1215059.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="ACHU0J_000185"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="ACHU0J_000185"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1215060.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="ACHU0J_000186"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="ACHU0J_000186"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1215061.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="ACHU0J_000187"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="ACHU0J_000187"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1215062.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53107..53286
                     /locus_tag="ACHU0J_000188"
     CDS             53107..53286
                     /locus_tag="ACHU0J_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1215063.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53372..53872
                     /locus_tag="ACHU0J_000189"
     CDS             53372..53872
                     /locus_tag="ACHU0J_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1215064.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53918..54424
                     /locus_tag="ACHU0J_000190"
     CDS             53918..54424
                     /locus_tag="ACHU0J_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR1215065.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="ACHU0J_000191"
     CDS             complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="ACHU0J_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1215066.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55362..55451
                     /locus_tag="ACHU0J_000192"
                     /pseudo
     CDS             55362..55451
                     /locus_tag="ACHU0J_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55479..56222
                     /locus_tag="ACHU0J_000193"
     CDS             55479..56222
                     /locus_tag="ACHU0J_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1215067.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56252..56791
                     /locus_tag="ACHU0J_000194"
     CDS             56252..56791
                     /locus_tag="ACHU0J_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1215068.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56896..57294
                     /gene="yciA"
                     /locus_tag="ACHU0J_000195"
     CDS             56896..57294
                     /gene="yciA"
                     /locus_tag="ACHU0J_000195"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1215069.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="ACHU0J_000196"
     CDS             complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="ACHU0J_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1215070.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58277..58573
                     /locus_tag="ACHU0J_000197"
     CDS             58277..58573
                     /locus_tag="ACHU0J_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1215071.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14062 a        15144 c        14643 g        14725 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataaacatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaactcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttc cctctatctt ctcgttgcgc ttaatttgac taattctcat tagtgactaa
    53041 ttttaatgag tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga
    53101 aataacatgg ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca
    53161 tccgacgcag gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa
    53221 cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa
    53281 tcctgattca ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt
    53341 atggctcgca gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt
    53401 tattcaactg ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa
    53461 actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga
    53521 aactcatgga cagattgaac gaattgatca agttgtggaa tcagaatcaa atctgaaaat
    53581 taagcgcatg aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga
    53641 aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga
    53701 gcattatgag attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg
    53761 taaagcagcg aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact
    53821 gactgatctg gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa
    53881 ttttaacttt tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca
    53941 tgactggtta cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat
    54001 ggccagccgt atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga
    54061 aaccaaaaac cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc
    54121 agtcattaaa gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt
    54181 cccttctgat gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat
    54241 cacctgttac acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc
    54301 gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat
    54361 tccgcaaaca actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa
    54421 ataataccct ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg
    54481 gaattaaaaa cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc
    54541 gtgttgctgt gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac
    54601 tgacatgtta accaaccagt cacggttaat cagataatca acccccacct gcccggcagc
    54661 tccccaggaa tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa
    54721 tccattatca aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct
    54781 gctggcatca ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacggttgc
    54841 aatatcgccg gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc
    54901 cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt
    54961 cacgctgaat ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt
    55021 tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag
    55081 aagagttgtt actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg
    55141 gttataaaaa cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac
    55201 attacatatg ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat
    55261 cctggttaat gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga
    55321 tcgccacttg ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag
    55381 atatgctttt ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc
    55441 ccgctaatta acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg
    55501 tataccgtga caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt
    55561 tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc
    55621 ttgcgcagct caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc
    55681 agaatatgtc accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt
    55741 cagaattaat cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt
    55801 ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca
    55861 agttatttat tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg
    55921 tcgttccggg aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg
    55981 acaaaatggg catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc
    56041 gtctggtggc acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg
    56101 cctcttcttt tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca
    56161 acctgatttc tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat
    56221 aaccttaagc atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct
    56281 ggttgtcttt ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat
    56341 cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat
    56401 ggccctgatc acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa
    56461 tgatgagttt attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt
    56521 agtcagccaa tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac
    56581 gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg
    56641 cggtctggca aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa
    56701 agtctttggc ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta
    56761 ccgccacatg ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc
    56821 tgtatctcct ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt
    56881 taagtcggtt ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt
    56941 actttagcca tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg
    57001 tcacaaatgg atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg
    57061 actgtgcggg ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc
    57121 tgctatgcac gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg
    57181 gtgaaaaaag tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt
    57241 aagtatgtag cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca
    57301 aaagcctccg gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat
    57361 tttaaacagg atattcacca caatcccact gcctggctta cccggctcat aacgccatct
    57421 gcgcatcgca tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg
    57481 tacgttatcc acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat
    57541 gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc
    57601 tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg
    57661 cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg
    57721 ttttggctgc tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg
    57781 cttttcaatg accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc
    57841 tggctctacc accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg
    57901 cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac
    57961 cgaggtatag agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg
    58021 ccaggggaag cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa
    58081 actaggcctc gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga
    58141 tcagtgaaaa tgtcattttt ggcgtaatta cccttacttt atgcatgacc ttaacaaaga
    58201 catcatcttt ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga
    58261 acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga
    58321 aaaacgcctt tccgttcgtc cggcacattt agcacgttta cagttactgc atgatgaagg
    58381 tcgtttgttg acagctggtc caatgccagc ggtagacagt aacgatccag gggctgcggg
    58441 atttactggc tcaaccgtta ttgctgaatt tgaatcgctg gaagcagctc aggcctgggc
    58501 cgatgctgac ccgtacgtag cggcaggagt ctatgaacac gtttcagtga aaccatttaa
    58561 gaaagtgttc tgag
//
LOCUS       DAZPDE010000004        55468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPDE010000004 DAZPDE010000000
VERSION     DAZPDE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.004"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            55..1541
                     /locus_tag="ACHU0J_000198"
                     /pseudo
     CDS             55..1541
                     /locus_tag="ACHU0J_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1556..2290
                     /gene="ypdB"
                     /locus_tag="ACHU0J_000199"
     CDS             1556..2290
                     /gene="ypdB"
                     /locus_tag="ACHU0J_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1215072.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2303..3160
                     /locus_tag="ACHU0J_000200"
                     /pseudo
     CDS             2303..3160
                     /locus_tag="ACHU0J_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3163..5665)
                     /gene="ptsP"
                     /locus_tag="ACHU0J_000201"
                     /pseudo
     CDS             complement(3163..5665)
                     /gene="ptsP"
                     /locus_tag="ACHU0J_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5690..6734)
                     /gene="ypdE"
                     /locus_tag="ACHU0J_000202"
                     /pseudo
     CDS             complement(5690..6734)
                     /gene="ypdE"
                     /locus_tag="ACHU0J_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(6734..7150)
                     /locus_tag="ACHU0J_000203"
     CDS             complement(6734..7150)
                     /locus_tag="ACHU0J_000203"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR1215073.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7147..7254)
                     /locus_tag="ACHU0J_000204"
     CDS             complement(7147..7254)
                     /locus_tag="ACHU0J_000204"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215074.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7215..7817)
                     /gene="ypdF"
                     /locus_tag="ACHU0J_000205"
                     /pseudo
     CDS             complement(7215..7817)
                     /gene="ypdF"
                     /locus_tag="ACHU0J_000205"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7832..9079)
                     /locus_tag="ACHU0J_000206"
     CDS             complement(7832..9079)
                     /locus_tag="ACHU0J_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR1215075.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9101..9427)
                     /locus_tag="ACHU0J_000207"
     CDS             complement(9101..9427)
                     /locus_tag="ACHU0J_000207"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1215076.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9646..10611)
                     /gene="glk"
                     /locus_tag="ACHU0J_000208"
     CDS             complement(9646..10611)
                     /gene="glk"
                     /locus_tag="ACHU0J_000208"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1215077.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10815..12071
                     /locus_tag="ACHU0J_000209"
     CDS             10815..12071
                     /locus_tag="ACHU0J_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1215078.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12186..12512
                     /gene="ypeC"
                     /locus_tag="ACHU0J_000210"
     CDS             12186..12512
                     /gene="ypeC"
                     /locus_tag="ACHU0J_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1215079.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12653..13891)
                     /locus_tag="ACHU0J_000211"
     CDS             complement(12653..13891)
                     /locus_tag="ACHU0J_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1215080.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14227..15429
                     /gene="nupC"
                     /locus_tag="ACHU0J_000212"
     CDS             14227..15429
                     /gene="nupC"
                     /locus_tag="ACHU0J_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1215081.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15479..17591)
                     /locus_tag="ACHU0J_000213"
                     /pseudo
     CDS             complement(15479..17591)
                     /locus_tag="ACHU0J_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17799..17874)
                     /locus_tag="ACHU0J_000214"
     tRNA            complement(17799..17874)
                     /locus_tag="ACHU0J_000214"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17839..17841),aa:Ala,seq:ggc)
     gene            complement(17914..17989)
                     /locus_tag="ACHU0J_000215"
     tRNA            complement(17914..17989)
                     /locus_tag="ACHU0J_000215"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17954..17956),aa:Ala,seq:ggc)
     gene            18210..18569
                     /locus_tag="ACHU0J_000216"
     CDS             18210..18569
                     /locus_tag="ACHU0J_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1215082.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18571..18963
                     /locus_tag="ACHU0J_000217"
     CDS             18571..18963
                     /locus_tag="ACHU0J_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1215083.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19015..20430)
                     /gene="gltX"
                     /locus_tag="ACHU0J_000218"
     CDS             complement(19015..20430)
                     /gene="gltX"
                     /locus_tag="ACHU0J_000218"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1215084.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20689..20764
                     /locus_tag="ACHU0J_000219"
     tRNA            20689..20764
                     /locus_tag="ACHU0J_000219"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20722..20724,aa:Val,seq:tac)
     gene            20809..20884
                     /locus_tag="ACHU0J_000220"
     tRNA            20809..20884
                     /locus_tag="ACHU0J_000220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20842..20844,aa:Val,seq:tac)
     gene            20889..20964
                     /locus_tag="ACHU0J_000221"
     tRNA            20889..20964
                     /locus_tag="ACHU0J_000221"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20922..20924,aa:Lys,seq:ttt)
     gene            20995..21120
                     /locus_tag="ACHU0J_000222"
     ncRNA           20995..21120
                     /ncRNA_class="other"
                     /locus_tag="ACHU0J_000222"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21240..21581
                     /locus_tag="ACHU0J_000223"
     CDS             21240..21581
                     /locus_tag="ACHU0J_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1215085.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21572..22498)
                     /locus_tag="ACHU0J_000224"
     CDS             complement(21572..22498)
                     /locus_tag="ACHU0J_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1215086.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22588..23586
                     /locus_tag="ACHU0J_000225"
     CDS             22588..23586
                     /locus_tag="ACHU0J_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1215087.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23583..23801)
                     /locus_tag="ACHU0J_000226"
     CDS             complement(23583..23801)
                     /locus_tag="ACHU0J_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1215088.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23803..25818)
                     /gene="ligA"
                     /locus_tag="ACHU0J_000227"
     CDS             complement(23803..25818)
                     /gene="ligA"
                     /locus_tag="ACHU0J_000227"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1215089.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25889..26875)
                     /gene="zipA"
                     /locus_tag="ACHU0J_000228"
     CDS             complement(25889..26875)
                     /gene="zipA"
                     /locus_tag="ACHU0J_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1215090.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27105..27866
                     /gene="cysZ"
                     /locus_tag="ACHU0J_000229"
     CDS             27105..27866
                     /gene="cysZ"
                     /locus_tag="ACHU0J_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1215091.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28051..29022
                     /gene="cysK"
                     /locus_tag="ACHU0J_000230"
     CDS             28051..29022
                     /gene="cysK"
                     /locus_tag="ACHU0J_000230"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1215092.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29406..29663
                     /gene="ptsH"
                     /locus_tag="ACHU0J_000231"
     CDS             29406..29663
                     /gene="ptsH"
                     /locus_tag="ACHU0J_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1215093.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29708..31435
                     /gene="ptsI"
                     /locus_tag="ACHU0J_000232"
     CDS             29708..31435
                     /gene="ptsI"
                     /locus_tag="ACHU0J_000232"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1215094.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31476..31985
                     /gene="crr"
                     /locus_tag="ACHU0J_000233"
     CDS             31476..31985
                     /gene="crr"
                     /locus_tag="ACHU0J_000233"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1215095.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32027..32878)
                     /gene="pdxK"
                     /locus_tag="ACHU0J_000234"
                     /gene_synonym="thiJ"
     CDS             complement(32027..32878)
                     /gene="pdxK"
                     /locus_tag="ACHU0J_000234"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1215096.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            32983..33357
                     /locus_tag="ACHU0J_000235"
     CDS             32983..33357
                     /locus_tag="ACHU0J_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1215097.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33390..34113
                     /locus_tag="ACHU0J_000236"
                     /pseudo
     CDS             33390..34113
                     /locus_tag="ACHU0J_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34317..35228)
                     /gene="cysM"
                     /locus_tag="ACHU0J_000237"
     CDS             complement(34317..35228)
                     /gene="cysM"
                     /locus_tag="ACHU0J_000237"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1215098.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35362..36459)
                     /gene="cysA"
                     /locus_tag="ACHU0J_000238"
     CDS             complement(35362..36459)
                     /gene="cysA"
                     /locus_tag="ACHU0J_000238"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1215099.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36449..37324)
                     /gene="cysW"
                     /locus_tag="ACHU0J_000239"
     CDS             complement(36449..37324)
                     /gene="cysW"
                     /locus_tag="ACHU0J_000239"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1215100.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37324..38157)
                     /gene="cysT"
                     /locus_tag="ACHU0J_000240"
     CDS             complement(37324..38157)
                     /gene="cysT"
                     /locus_tag="ACHU0J_000240"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1215101.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38157..39173)
                     /gene="cysP"
                     /locus_tag="ACHU0J_000241"
     CDS             complement(38157..39173)
                     /gene="cysP"
                     /locus_tag="ACHU0J_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1215102.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39477..40268)
                     /gene="ucpA"
                     /locus_tag="ACHU0J_000242"
     CDS             complement(39477..40268)
                     /gene="ucpA"
                     /locus_tag="ACHU0J_000242"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1215103.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40397..41255)
                     /gene="murR"
                     /locus_tag="ACHU0J_000243"
                     /pseudo
     CDS             complement(40397..41255)
                     /gene="murR"
                     /locus_tag="ACHU0J_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41419..42315
                     /gene="murQ"
                     /locus_tag="ACHU0J_000244"
     CDS             41419..42315
                     /gene="murQ"
                     /locus_tag="ACHU0J_000244"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1215104.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42319..43743
                     /gene="murP"
                     /locus_tag="ACHU0J_000245"
     CDS             42319..43743
                     /gene="murP"
                     /locus_tag="ACHU0J_000245"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR1215105.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43748..43834
                     /locus_tag="ACHU0J_000246"
                     /pseudo
     CDS             43748..43834
                     /locus_tag="ACHU0J_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43923..44822)
                     /gene="yfeX"
                     /locus_tag="ACHU0J_000247"
     CDS             complement(43923..44822)
                     /gene="yfeX"
                     /locus_tag="ACHU0J_000247"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1215106.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(44918..45493)
                     /locus_tag="ACHU0J_000248"
     CDS             complement(44918..45493)
                     /locus_tag="ACHU0J_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1215107.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45554..46003)
                     /locus_tag="ACHU0J_000249"
     CDS             complement(45554..46003)
                     /locus_tag="ACHU0J_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1215108.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(45990..46415)
                     /locus_tag="ACHU0J_000250"
     CDS             complement(45990..46415)
                     /locus_tag="ACHU0J_000250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1215109.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46629..47498
                     /gene="amiA"
                     /locus_tag="ACHU0J_000251"
     CDS             46629..47498
                     /gene="amiA"
                     /locus_tag="ACHU0J_000251"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1215110.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47502..48401
                     /gene="hemF"
                     /locus_tag="ACHU0J_000252"
     CDS             47502..48401
                     /gene="hemF"
                     /locus_tag="ACHU0J_000252"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1215111.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48407..49459)
                     /gene="eutR"
                     /locus_tag="ACHU0J_000253"
                     /pseudo
     CDS             complement(48407..49459)
                     /gene="eutR"
                     /locus_tag="ACHU0J_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49505..50005)
                     /gene="eutK"
                     /locus_tag="ACHU0J_000254"
     CDS             complement(49505..50005)
                     /gene="eutK"
                     /locus_tag="ACHU0J_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1215112.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(50018..50677)
                     /gene="eutL"
                     /locus_tag="ACHU0J_000255"
     CDS             complement(50018..50677)
                     /gene="eutL"
                     /locus_tag="ACHU0J_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1215113.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50687..50755)
                     /locus_tag="ACHU0J_000256"
                     /pseudo
     CDS             complement(50687..50755)
                     /locus_tag="ACHU0J_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51859..52650
                     /locus_tag="ACHU0J_000257"
     CDS             51859..52650
                     /locus_tag="ACHU0J_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215114.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53370..53765
                     /locus_tag="ACHU0J_000258"
     CDS             53370..53765
                     /locus_tag="ACHU0J_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1215115.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53783..54046
                     /locus_tag="ACHU0J_000259"
     CDS             53783..54046
                     /locus_tag="ACHU0J_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215116.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54069..54611
                     /locus_tag="ACHU0J_000260"
     CDS             54069..54611
                     /locus_tag="ACHU0J_000260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215117.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54750..55037
                     /locus_tag="ACHU0J_000261"
     CDS             54750..55037
                     /locus_tag="ACHU0J_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215118.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55076..55282
                     /locus_tag="ACHU0J_000262"
     CDS             55076..55282
                     /locus_tag="ACHU0J_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215119.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13841 a        14721 c        13721 g        13185 t
ORIGIN      
        1 catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc acccgtgcac
       61 gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct tatctgcctg
      121 ttctttctca tccgtatccg cctgtttcgc gaactgttgc acaagtcggc gcactcccca
      181 aaagaattgc tcgccgttac cgccattttc tcgctgttcg ccctgttcag cacctggtcc
      241 ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat gtccggcggg
      301 attctgtttg gcccgtgggt aggcatcatt accggcgtga ttgcgggtat tcaccggtat
      361 ttaattgata tcggcggcgt gacggcgatc ccctgcttta tcaccagcat tctggcgggt
      421 tgtatatcag gctggataaa cctgaaaatc cccaaagcac agcgctggcg ctggatatcg
      481 ccaacaaaac gctaccgctg tttcgccatg tcaatagcga gtcattacgc aaggtctgcg
      541 aaattatccg cgatgacatt cacgccgatg cggtggcgat tactaatacc gatcatgtgc
      601 tggcctatgt tggcgtgggt gaacataact atcagaatgg cgatgacttc attagcccga
      661 ctacccgtca ggcgatgaat tacggaaaaa tcatcattaa aaacaatgat gaagcccacc
      721 gcacaccaga gattcattcc atgctggtga tcccattgtg ggagaaaggg gtcgtgaccg
      781 gaacgctgaa aatttactac tgccacgcgc atcagatcac ctcgtcatta caggaaatgg
      841 cggtcggtct gtcgcaaatc atctccacgc aactggaggt ttcacgcgcc gagcagctac
      901 gtgaaatggc aaataaggca gagattcgcg ccctgcaaag caaaattaat ccccattttc
      961 tgtttaacgc tctgaacgct atttcatcgt caatccgtct gaatccggat accgctcgcc
     1021 agttgatctt taatctgtcg cgttatctgc gctataacat tgaattaaaa gacgatgagc
     1081 aaatcgatat caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc
     1141 gctttggcga caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc
     1201 ccagcctgct gatccagccg ttggtggaga acgccattgt ccacggtatt cagccttgca
     1261 aaggtaaagg cgttgtcacc atcagcgttg cagagtgcgg aaatcgggta cgcattgcgg
     1321 tgcgagatac cgggcacggc atcgttccaa aggtgattga gcgggtcgaa gcgaatgaaa
     1381 tgccgggcaa taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg
     1441 aggggctgca tatccgccgc ctggagccgg ggacggaaat tgcgttttac attcctaacc
     1501 aacgcacccc agtcgcctca caggctacgt tattgctttg agccggagtg atattgtgaa
     1561 agtcatcatt gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga
     1621 gcacagccag atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt
     1681 gcagcataac cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggcgt
     1741 gttgctggcg caaaacatca gccagttcgc ccataaaccg tttattgtgt tcatcaccgc
     1801 gtggaaagaa catgcggtag aagcgtttga actggaggcg tttgactaca ttctcaaacc
     1861 gtatcaggag tcacgtatta ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca
     1921 gcagaccagc agtacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt
     1981 gaaagatgag cgaataatcg tcacgccaat taacgatatc tattacgccg aagcgcacga
     2041 gaaaatgacc tttgtctata cgcggcgtga atcctacgta atgccgatga acattaccga
     2101 attttgcagc aaactgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa
     2161 tctgaacaaa atacgcgaaa tcgaaccgtg gtttaataac acctacattc tgcgactgaa
     2221 agatctggat tttgaagtgc cggtcagccg cagcaaagtg aaagaatttc gccagttaat
     2281 gcatctttaa tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt
     2341 cgccgatctg ttcagcggcc tagtgcttaa cccgcaacta ctggggcgcg tctggtttgc
     2401 cagccagcct gcctcgttac cggtgggcag tttatgtatt gattttcccc gtctggatat
     2461 cgtgctgcgc ggcgaatacg gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg
     2521 agaaatgctg tttattccgg cacgcgcggc taatttaccg atcaacaaca aaccggtgat
     2581 gctgttaagc ctggtgttcg caccgacctg gcttgggtta tcgttttacg atagccgcac
     2641 cacgtcgttg ttgcatcctg ctcgccagac ccagcttccc agcctgcaac gcggtgaagg
     2701 tgaagcgatg cttaccgccc tcacccatct tagccgttcg ccgctggagc agaatatcat
     2761 tcagccactg gtgttaagtt tgctgcatct ttgccgcaac gtggtgaata tgccgccggg
     2821 taattcacaa ccgcgcggcg attttctcta tcacagcatt tgtaactggg tttaggataa
     2881 ttatgcccag ccgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgcccaatca
     2941 tctgtcaaaa ctgtttgctc agcatggaac gatgcgtttt atcgagtatg tgcgttgggt
     3001 gcgaatggcg aaggcgagga tgattttgca gaaatatcat ctgtcaattc atgaagtggc
     3061 acagcgttgc ggttttccgg atagcgacta tttttgtcgc gttttccggc gtcagtttag
     3121 tctgacgccg ggagagtaca gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt
     3181 ttccagcagc gtcaggatac tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt
     3241 tttattcacc agtttacgcg ccagctgcga gaagactttc acatgattca tcccttcgtt
     3301 agcacccagc gtcagcatga tcaccagttc gacttcgccc atttctgact gccagtcaac
     3361 cggtttcgcc agccgggcaa tgctgatact ggaatgacgg atccactgag atttggtgtg
     3421 cgggatcgct acgccaaaac caacgccggt ggtaacaatc tcttcccgct gccagacatc
     3481 ttcttccagc tcaaacggat gttcagtgcg cccgttaacg ccgaggttgc cgcacaggaa
     3541 ctggatcgcc tgctctttat tgctaaaatc ctgatcaaca aagatattct ccagcgccag
     3601 cagtgggcga acgtcttctt ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc
     3661 actgcggcat tcacatgcct gacgcgccag ttcccgacac gcctcgctat ccagttgacg
     3721 aagctggctt ttcaccgccg gaatacgcgg gctactcata ctcagctcat ccaggcccag
     3781 cccaagcagt agcggcagat aacggctttc accgcccagt tcaccgcaaa tgcctaccca
     3841 tttgccccgc tgatgcgcag tggtaactat ttgctgcaac atgcgcagga acgatggcgt
     3901 aatcgggtta tatagcggcg atacgcgcgg gttattacga tcgaccgcat acagatactg
     3961 ggtcatatcg ttggagccga tactgaagaa atcgacctca tcgcagaagt ggtcgatgat
     4021 gtagcacacc gacggcactt ccaccatgat cccaagcgta atcgtctctg catgacgcag
     4081 gccatcgcgc ttaagctcaa cgatcgcttt ttggatctcg cctttcaccc ataagatctg
     4141 atcgaggctg tgaaccatcg ggatcatcaa ctgggcgttg ccgaaactgg cggcgcgcaa
     4201 aatggcccgc agttgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg
     4261 atagccgagg aacgggttct cttcctgggg aatattcaga taaggaatgc ttttatcgcc
     4321 gccgatgtcc atcgtgcgga agataatcgg cttgtcgccc gccgcccgcc gccagtagca
     4381 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
     4441 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
     4501 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
     4561 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
     4621 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
     4681 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
     4741 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
     4801 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
     4861 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
     4921 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
     4981 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
     5041 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
     5101 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
     5161 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
     5221 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
     5281 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
     5341 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
     5401 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
     5461 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
     5521 agcttttagc atcagccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
     5581 actggctgca ctgttcttta agttcccacg ccggacgagc atgtagaccg ttaggcaaag
     5641 gacagagaaa ttgaatcgtt aacataaccg ctccgggtta atcaggagat catctgaaat
     5701 ccgtcagttg aacaaccgtc tcacgcgtaa gacgttgaat aagggcagat aaaagttgct
     5761 ccatctgcaa aatgtcgcgg caatcggcaa tcggcaatcg atgccgcgca atgtccgtgg
     5821 cgggtggcag gccccattac caatgtgggt acgccagtac cggttaagtg caccgctcca
     5881 ccgtccgtgc cgccgttact gaacatatcc gcctgcaacg gaacgccaat ttctgccgcc
     5941 acggtttcaa tccacgcggt aagttttggc ggcgcaatca gcgatttatc gcttaacacc
     6001 agcatcggcc cgttaccaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc
     6061 cagcaggcgg tatcaagcac aatggcgacg tccggcgaca ccgcgcgggt agcagtttgc
     6121 ccgccgcgta atcccacctc ttcgctggaa cttgccacca gccacacttc cgcaggcagt
     6181 tcggcgtcgt gcagctcacg tagtaacgtc accagcagat agcaactgag gcgatcatca
     6241 aaggctttgc ccatcacccg ctggtgaggg aggacctgaa aagtggtatc gaaggtgacg
     6301 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat aggtgcgcgc gccaatgtcc
     6361 acgcgcatgg cgctaacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg
     6421 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg
     6481 ccaaccggca gcacgtcaat agcgccttcg cgggagatgc tgcgcaccat aaatcccact
     6541 tcgtccatat gggcgcagat catcaccttc ggacctgtcg attcattgag gcggatcagc
     6601 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gagccgcttc ttccagcagg
     6661 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
     6721 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
     6781 cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgcccta
     6841 gcaaataaat ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc
     6901 gcggtgaaaa acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac
     6961 cgaaatagtc gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct
     7021 ggcagcgcac gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct
     7081 gatagacgtc aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
     7141 tgcgcgtcat atcagcagta gccctgatac agcgcaccaa aatcgagagt gacaaactcg
     7201 cccgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccgcg ccagccgctg
     7261 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     7321 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     7381 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7441 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7501 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7561 gcaatgattt gattgacgat agtggtaagc gtgttcgttg cgtcaagcaa atgcagctgg
     7621 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7681 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7741 ttctgccgtg aggagagaag caccgcatcc agttgttgca ccttaagcca gttgcgcagc
     7801 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt
     7861 tgcggaacat catcagacgc aggaacacca ccatcagcgc agtaatgatt gcccccagcg
     7921 cgatccccgc catataaacg cccaagttag tgaccagcgg ccatgcccag atagcggatt
     7981 ccgggaacca ttgcactgcg cccagccaga cagctgcggt tgagccgaca atcgcgccga
     8041 ccatatatga cggaatagcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     8101 taatccccat aaaggccagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     8161 acaggcgttt gccggttaaa cgacggtcaa taatggtcgc cagacccaga ccaatcggcg
     8221 gaataacgat agcgatagaa cgagcggtga ccggtaatac gtggtcagtg gtaaagctga
     8281 aggcaacgaa acctgcggct ttgttgatcg ggccaccaag gtcgattgct gtcgccgcgg
     8341 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acagtacgga
     8401 taccgccgtt gatccagcca ccaaagggag tgatgacgta gtacatcgcc agcatgacaa
     8461 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8521 actggatttt ctggttcatc catttcacaa ggtaaccggc aacgatagaa ataatcagcg
     8581 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8641 agttcagcag ttgcgtcggc tgggtagaca tcaacccgcc gataaaacca gccgggaacg
     8701 ccagtttgcc accgatagag ttcgccacaa atgcagcaaa catcggaatg gcaaaaccaa
     8761 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt aagtaaggag aggtcgaaac
     8821 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8881 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8941 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     9001 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     9061 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     9121 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     9181 tttcataaac gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggaat
     9241 ggatgatgat ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgg atgccgtccg
     9301 cgccctgggt ttcaattttc acttcataac cggcttctac cgccgcttct tccagcacct
     9361 gagcggccat aaaagtgtga gccaggccca ttgggcaggc acataaggca atcagtttct
     9421 tactcatcgt taattcctcg ttcagtgata gttgactagt gaaattatga acgtgcaaaa
     9481 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9541 gtgtgacgca ggtcggcctt gtgaggggac acaaagaaaa attgaattaa ctgtatgatt
     9601 taaaagatta tcgggagagt tacctcccga tataacagga aggatttaca gaatgtgacc
     9661 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggatagtcat ggacgatgag
     9721 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9781 gaaaccggag gctttgaaga actcaaggaa gcgcggcacg ataccgcccg caataaacac
     9841 gccgccaaat gtcccgagat tgagcgccag attgccgcca aaacggccca taatgacgca
     9901 aaacagcgac aatgcgcggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9961 atcttttggc ttgagatttt ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa
    10021 attcaccagc ccagggccag aaagcacgcg ctccgccgaa acatgaccaa tttccgcacg
    10081 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
    10141 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
    10201 cgttccggca ccgtaaaccg caataggctt accttcgacc ggttctgcgc caccaaactg
    10261 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
    10321 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
    10381 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10441 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10501 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10561 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10621 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10681 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacaatta ctttatcgtt
    10741 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10801 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10861 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10921 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10981 ccctctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    11041 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccggttc
    11101 cgccctctgc gctacctaga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    11161 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    11221 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    11281 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    11341 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    11401 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11461 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11521 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11581 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11641 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11701 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11761 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11821 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11881 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11941 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    12001 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    12061 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    12121 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    12181 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    12241 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    12301 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    12361 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12421 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12481 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12541 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12601 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12661 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12721 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12781 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12841 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12901 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12961 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    13021 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    13081 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcacca
    13141 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    13201 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    13261 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    13321 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    13381 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagcggtt
    13441 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13501 cctgcaacaa cgaaacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13561 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13621 gcggatagtg atcacgaatc tgctccgcca gatttttacc ggtggcaatc cctagtttgg
    13681 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13741 gatagccgaa gctagcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13801 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttacgc gccgcccgtc
    13861 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13921 tgtttcatgc tatgctaaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13981 ttctctgata gaccaagatt atgattacga aaagattgat gacttgaatg ttatggttat
    14041 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    14101 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    14161 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    14221 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgt gattctcgca
    14281 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    14341 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    14401 ggcttctccg aaatgttcga aaaactgctc ggatttgcca acgaagggac taacttcgtc
    14461 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14521 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14581 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14641 gccgtcagct ccctgattct gggtcagtca gaaaacttta ttgcctataa agatatcctc
    14701 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14761 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14821 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14881 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14941 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    15001 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    15061 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    15121 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    15181 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    15241 gctgaaggca tcatctctgt gttcctagtt tccttcgcta acttctcttc aatcgggatt
    15301 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    15361 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15421 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15481 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15541 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15601 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15661 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15721 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15781 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15841 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15901 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15961 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    16021 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    16081 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    16141 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    16201 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    16261 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    16321 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    16381 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16441 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatac aacttttcgt
    16501 tttcctgcat caacggcagc agcgtacggc aaattgagcg gatacagtga acgcgcatca
    16561 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaaactct
    16621 tgcccgcctc ttgctccggc gcttgttcca acgcacgaaa gttgggtagg agcgttaacg
    16681 gatctgttag cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc
    16741 gcaccatata gagcaggcaa acgctaaagg aaatcagcac cgcgagaata aatgccagcg
    16801 aatattcggt ttccacccct tgcaaaaagt tctgattgta attcagaagg caaagcgttg
    16861 aaacagccca ggtgagattt aaaaacggat agcgaagatt accgaccccg agggtaaaga
    16921 tgatgaagaa aacgggtacc aggtaaccgg caataaagtc gttttcataa ggtgtgcaca
    16981 acagaagtag cagcacgctt aacgctgcca gccagcttaa ggtaaatgcg agtgagatag
    17041 tagaaaagca tgttgtaaat cagcactgcg gtgaaaaggc ttagtaaatc aacgaccgtg
    17101 aaaatggcat ccgcatcgcc gaaaaaggtg gatatcttta gcgggaaatc aaagaagctc
    17161 cccacaagat acatgctgca tttgatgccg atcggcgtca ccaaaccaag ccagaacaga
    17221 cgttgccaga catatcggct ggtcaatcca taacgccaac gcgtccccag ctgccagcgt
    17281 aatattgcgc aggcacacaa cacgacaaac gtctggctgc cgagcatgac cgcagtctgt
    17341 aaaaaagaga gatgaaagtt ccacagattg gtgcaaaaca tcccgcataa aacgggaacg
    17401 acaccgcgcc agccaaagat aaacaagatt gacagcatta cgcacagtgg catccacgcc
    17461 agaaagatat aactggaatg gataatggca agtggcgaaa taaaacggga tagctgaata
    17521 agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga tattttttat caggttatgc
    17581 tccacaaaca tgagtacact actctcactg atcggggttt tcaggcacac aaataataag
    17641 tttattcgca tcgcgaagca aacaggggcc agcctgttgc cgatttttca tccaatgatg
    17701 ctttcaccag gcggcgctaa cccggacgta gactgtatta caaaagcggc aaaaagcaga
    17761 gacaaaaaac ccccgctttg cagcgagggt tggaaatttg gtggagctaa gcgggatcga
    17821 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccacgatg
    17881 cgtttacgta ccaaatttgc tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc
    17941 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagccccg tcacgtaaag
    18001 cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg
    18061 caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg
    18121 cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa tgaactacta
    18181 atagacccac atacattcag ggaattgtta tgttcaagga gaggatgaca ccagatgaac
    18241 ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct
    18301 ggacaacgtc accaaaacct ggcgtccagg gtggcaaagc ccgactggtt cacgtcaatg
    18361 aacaggttcg tgaatatatt cgcaatgctg aacgtccaga aggtcaggga gaagcgcctg
    18421 ccctttctgg tgatgcgccg cttgaagtat tgctggtgac actggcgaaa gagatgacgc
    18481 cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac
    18541 aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa aatgaccacc
    18601 gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gaccagagaa
    18661 aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg
    18721 gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa cacaccaatg
    18781 ttgatggaag ctgaagagag aattgctgaa tacgctcctg gagcacgcgc cccggcgtat
    18841 cgacaaatca tcaacgcgat agataatatg acagacattg agcaggaaaa agtcgcgcaa
    18901 tttttgtcac gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga cgaatcagca
    18961 taaataaaaa acggcaggat attatctcct gccgtttatc tttttacacg ctaattactg
    19021 ctgattttcg cgttcagcaa taaaatccag cgctttgttg atacgctcga tactgcgggt
    19081 cttaccaatt gcgtgaacgg taacatccag tgctggagac tgccccgcac cggttacggc
    19141 gacacgcagc ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg
    19201 aatagcgtga tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt tgtcacgaac
    19261 cacttccagc ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat cggcatcgaa
    19321 ctcagcaaaa tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca
    19381 gcgttcgccc agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt
    19441 ttcctgctca atgtgccact gtaagtgagt agcaacatac tccggcggca gcgcgttaat
    19501 gtagtgatgg ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac
    19561 ggcattcaaa gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg
    19621 ggaccagccc agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg
    19681 atactgcatt acgctgactg ccccgtgacg tttggacagt tttttaccgt catcgccatt
    19741 gatcatagaa acgtgcgcgt aaaccggcac cggcgctttc agggccttaa gaatgttgat
    19801 ctggcgcggc gtgttgttga tatggtcttc gccacggata acgtgggtga tttccatatc
    19861 ccagtcatcg acaaccacac agaagttata ggttggggaa ccatcggtac ggcggataat
    19921 aagatcgtcc agttcctggt tgctgaactc gatcggacca cggatctgat cgtcaaaaac
    19981 aacagaacct tcctgcgggt tagcaaaacg tacaacacac ggttcatcat cagcatgatg
    20041 ctcatggctg tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc
    20101 ttcgcgcagc gcttccaggc gctctttaga gcaatagcat ttataagcag tgccctcttc
    20161 cagcatctga tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt agtacggacc
    20221 ttcatcccac tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg
    20281 cgtggaacgc tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtggttacg
    20341 tgcaaaaagc caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg
    20401 gcttggcgcg aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga
    20461 caaccggcaa atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct
    20521 caatgtagtt cgggtttcta ccttacgcat tattgttttc gttgacaaat tgcgcaacac
    20581 ggtctgtttt gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat
    20641 gcgttgactc attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct
    20701 cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc
    20761 accaactact ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct
    20821 cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc
    20881 accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag
    20941 gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttgctat
    21001 ctgcgatact caaagattcg aacctgcagc aggtttgagt tgagcgcagc gaaacaacgg
    21061 agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca cgacccacca
    21121 atgcaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag attgattttt
    21181 cttctcaagt tccttgttct tctaccgata ttacctcgta cagtgacgag gactgaacaa
    21241 tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt aacagggcat
    21301 cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc atcaaactca
    21361 ctcaacaaat taaagagatt gtagacacta gcggcagtgc tgaagacaag caaaaacagg
    21421 ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag ttgcagaaac
    21481 agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc tctgaatgta tcccttctaa
    21541 atcctgttga aaaaacgact catatcgata tctatatctg atacaacgga tccccttccc
    21601 gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct tcagcggcgg
    21661 tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaacc accggcgtaa
    21721 tgcgcttaac caccagtggg ctaccttcag gcaatggtag cgccagtgca ggaagaatac
    21781 tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca atctcctgca
    21841 caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg
    21901 acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt acataatctt
    21961 ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa agaatcgctt
    22021 cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg acatctccct
    22081 ggcgaatact ttccatcacc cactgctgcg gacgatcatg caggacaaac tgaatatctg
    22141 gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa atggtctggc
    22201 tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta tcgcgcaacg
    22261 tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc cctgcatctg
    22321 taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga ttttccagct
    22381 ccttcacact gtgacttact gccgactggc tcaagccgat acgctctcct gcacgactaa
    22441 aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa taattcatct
    22501 gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga agaagcacaa
    22561 tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga tcctttcacc
    22621 ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg cgatttcgtc
    22681 cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat gcacggcgcg
    22741 aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca tttgtgggta
    22801 atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg
    22861 gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct cccggctacc
    22921 gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt
    22981 tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt tggtctggtg
    23041 atgaatgttc acggtgcaag gggcagcctt gagcaggtcg gtaaaattat gctgcaactg
    23101 ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc
    23161 aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt ttatacagcg
    23221 ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc attgctgttt
    23281 atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt ctttatggca
    23341 cgccgactga gcttcaataa ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag
    23401 agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt gatcggtatg
    23461 atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc ggtgctggcg
    23521 cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag cgccgataaa
    23581 gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt tttaatcagc
    23641 agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt gcgggcaaac
    23701 cacagcagat actcctccgg caggtcaatt aagcgacgcc ctttgtattt gccaaacggc
    23761 attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac ccagcaaacg
    23821 cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg ccagtttaga
    23881 tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc ccgcgacttt
    23941 cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc ttaagctgcc
    24001 cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat
    24061 cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc ggttgctttc
    24121 ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag gcaccttttg
    24181 cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg ccgccagacc
    24241 tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc gagcaaaggt
    24301 ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccaattttg gccccatacg
    24361 ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag tatggacata
    24421 ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg
    24481 gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca ggccaccggt
    24541 acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata ccggacaatg
    24601 cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga cgttaaccac
    24661 ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc gtaaaccaag
    24721 acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc cagcaacatg
    24781 gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct gaaactcgac
    24841 gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgcaaccg cccagcgcgg
    24901 ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct taatcaccac
    24961 gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat agaacgccag
    25021 cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaccc cccacttttt
    25081 aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca gaacaccaac
    25141 gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag
    25201 tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc gtcgcgcatc
    25261 ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca cttccagacg
    25321 cgccgggata ttctctccgt gcagcttcag cggaatggcg cgaatagtac gcacattaga
    25381 cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta aaacgccatt
    25441 ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc aggtgacttt
    25501 ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc tttcttcatc
    25561 aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga aagctgccag
    25621 cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg gatgtttggt
    25681 ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc
    25741 atccatcaca tgataaagat attcatgatg gcaaagcgtc gttcgcagtt ctgtcagttg
    25801 ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa gcgggggttc
    25861 gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact tcgcggatga
    25921 tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg tcaagcacga
    25981 caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg aagttctgca
    26041 gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta gtgaaatcct
    26101 tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat aacgccgggc
    26161 cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa atgaagcccg
    26221 cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga tgcgccgcga
    26281 cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga gccggttccg
    26341 ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg
    26401 gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct
    26461 gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc gctggacgcg
    26521 gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga cgcggctgcg
    26581 cagacgcata aggcggtagg tactggtgtt gcggcgacgg acgagcagcc tcatgctcct
    26641 gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg ccctcatcat
    26701 cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt tttaatggcc
    26761 gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt accagtaaag
    26821 cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc atcattatat
    26881 tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag agtatttgcc
    26941 gattacctca agtgcaagtg cactattaac tttcacagca caaagataga tgaaatcgtg
    27001 ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc cggtaaaata
    27061 cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt tcatcattca
    27121 catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc gtctcgcaac
    27181 ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg atggggggcg
    27241 cattctggtg gctctttaca cagctcgatg tctggatccc gactttcatg agttacgttc
    27301 cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc tctgtgctgt
    27361 tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg tttaacggtt
    27421 tattggctga acaactggaa gcacgattga ctggcgctac accgccagat accgggattt
    27481 tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa tttgtctggt
    27541 atctgccgcg cgcaattgta ttactaattc tttacttcat ccctggtatt gggcaaaccg
    27601 tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag tattgcgatt
    27661 accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg cgcacacgca
    27721 aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc ccgctgctta
    27781 atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc gattgctatc
    27841 gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac cgtttgtgtg
    27901 aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt tcccttctgt
    27961 atatagatat gctaaatcct tacttccgca tattctctga gcgggtatgc tacctgttgt
    28021 atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga taactcgctg
    28081 actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg cattctggcg
    28141 aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc caacatgatt
    28201 tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga accgaccagc
    28261 ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa actcaccctg
    28321 accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt aggtgcaaac
    28381 ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc agaagaaatt
    28441 gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc ggcaaaccct
    28501 gaaattcacg aaaaaaccac cggtccggag atctgggaag ataccgacgg tcaggttgat
    28561 gtatttattg ctggcgttgg gactggcggt acgctgactg gcgtcagccg ctacattaaa
    28621 ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga ttctccagtt
    28681 atcgcccagg tgctggcagg tgaagagatt aaacctggcc cgcataaaat tcagggtatt
    28741 ggcgctggtt ttatcccggc taacctcgat ctcaagctgg tcgataaagt cattggcatc
    28801 accaatgaag aagccatttc taccgcccgt cgtctaatgg aagaagaagg tattcttgca
    28861 ggtatctctt ctggagcagc tgttgccgca gcgttgaaac tacaagaaga tgaaagcttt
    28921 accaacaaga atattgtggt tattctacca tcatcgggtg agcgttattt aagcaccgca
    28981 ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt aatgccagct tgttaaaaat
    29041 gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt tcgcccctcc
    29101 tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt tgatgcgcga
    29161 aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt tcaattcttc
    29221 ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc cgccagcaat
    29281 ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa caatacaggc
    29341 taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata agttggggaa
    29401 atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac acccgccctg
    29461 ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg acttccaacg
    29521 gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg actcaaggta
    29581 ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa catctggtta
    29641 aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc acaagtaagg
    29701 tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta aagctctgct
    29761 tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc aggttgatca
    29821 ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg aaacgatcaa
    29881 aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag ggcatattat
    29941 gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag ataaacacat
    30001 gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc tggaagagct
    30061 ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta agcgcctgct
    30121 gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg aagtcattct
    30181 ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga aggtgctggg
    30241 tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc gttctctgga
    30301 actgcctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg acgactatct
    30361 gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag ttattgataa
    30421 aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta aactgaaaga
    30481 tctgccagct attacgctgg acggtcacca ggtagaagta tgcgctaaca ttggtacggt
    30541 tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt atcgtactga
    30601 gttcctgttc atggaccgcg acgcgctgcc cactgaagaa gaacagtttg ctgcttacaa
    30661 agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt cgtaccatgg acatcggcgg
    30721 cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc tcggctggcg
    30781 cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc gatcagctcc gcgctatcct
    30841 gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct ctgttgaaga
    30901 agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg acgaaggtaa
    30961 agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg ccgcaacaat
    31021 tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg atttaacgca
    31081 gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc agccaatgtc
    31141 accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg aaggcaaatg
    31201 gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt tgctggggat
    31261 gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga agattatccg
    31321 taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc aaccgacaac
    31381 ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct gctaatccac
    31441 gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat aaactgaaat
    31501 ctctggtttc cgacgacaaa aaggataccg gaactattga gatcattgct ccgctctctg
    31561 gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa atcgttggtg
    31621 atggtattgc tatcaaacca acgggtaaca aaatggtcgc gcctgtagac ggtaccattg
    31681 gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc tgatagcggc gttgaactgt
    31741 tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag cgtattgctg
    31801 aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg ccgctgctgg
    31861 aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac gaaatcaaag
    31921 aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc cgcatcaaga
    31981 agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttat gcttccgcca
    32041 gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc acttccagta
    32101 cgcgcaaccc cgcacggtgc accgcatcgt ttaacgcctt ccctttcagc aaaccactga
    32161 taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc cgtgaatggg
    32221 aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga ttttcttcat
    32281 taccggaggc gctggtaatc accacccatt ttaatgtgtc tgaaagcaga ctttttgcgg
    32341 cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc agctcaaaga
    32401 tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgcc tcgggaaggt
    32461 caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc atgatcaata
    32521 ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg atttgcgatg
    32581 ccgttcccat atagcccgtg gttacagcac gaagttggcg cagcgcatca cgctcctgaa
    32641 gcgcacgcaa atagccgcta aaccattcgt ccggaatcgc accaccgtag aaagtgtcat
    32701 aatgcggcgt attgctcagc aataccgtcg gcacggcaaa gacattcaag ccgttctgtt
    32761 ttatagcagg caccgcaatg ctgttgccta cgcttccgta cactacctgc gactgcacgg
    32821 cgacgatatc cgcctgcagc gccctactct tatcgttaaa caacaacaaa ctactcattt
    32881 aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta cctgattctg
    32941 gcagtcaaat cggctattac aaaacaaaga taaggtaatt cagtgaagaa aatcatttgt
    33001 ctggccatta cgctgctaat gacactcccc gcttacgcga agttaactgt tcatgaagaa
    33061 gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat atttgtgcgc
    33121 aacggtgatg aacatacctg cgatgaagcg gtttcccacc tgcgtctgaa gctcggcaat
    33181 acccgtaacc gcattgacac tgccgagcag tttattgata aggtagcttc gtcgtcatcg
    33241 attaccggga agccgtatat cgtgaagatc cccggtaaga gcgatgagaa cgcacagcct
    33301 tttttacatg cgttaattgc gcagacggat aaaacggtgc ctgcggaagg aaattaatcc
    33361 gctttggtaa ggcatttaca ggaggtaaca tgaaaaaatg ctttatttat cacgatgaaa
    33421 aatcgaataa attttggtgg atagattacg aaggggatag tttagctgtc aactatggca
    33481 aggtaggtag tattggtaaa ttccatacaa aagaattcga taatgaagaa cagtgtctga
    33541 aagaagccag taaattgatt gccgcaaaaa tgaaaaaagg atatcaagaa actccagact
    33601 ttaacttcat ggatctctac tattttgatg atgaagagat tgggttacat cttaaaacgt
    33661 cacacccaaa cttccagtgc cattttactg atccacttta tatgtgttgc tgggatgaag
    33721 aatctccttt tggtagcgat gaaggtgctg atgctctaaa cgaacttgaa aatagcctcc
    33781 gttctggact gtgctgattt ccctcaaatg ttaattgaaa ctatgtgggg tatgaaatac
    33841 atcgctatgg acagtattct tgaagaggat gtgcgtgcgc aattactagc cgatgaaatg
    33901 agctctatcc agagcaatat gattacctac gcgactgcat tcggtcagat caaagtcatg
    33961 ggtaaaatct cccataaact taaaaagatg ggactcaatg cactagcgcg tcatcagctt
    34021 accgcaaaaa ttcttcaatg gggtgacggt caggactcac caatacttca aaaaatgatt
    34081 gatgacctta ctgcttttcc acacgaaaat taaaaactgc atttgtcggt agcaacaact
    34141 gccgacaaat cagattattg acaaagtgca ctttgtttat gccggatgcg gcgtaaacgc
    34201 ctgatccggc ataaaaatat tgctaattca atatattgca gaaacattgt aggcctgata
    34261 agcggagcgc atcaggcagt tttgcgtttg tcatcagtct ccaatgctat taatccttaa
    34321 atccctgccc cctgactaaa atgctcttcc ccaaacactc cggtggagag gtagcgatcg
    34381 ccacgatcgc agatgatcgc taccaccacc gcaccagggt tagcttttgc cacccgcagt
    34441 gctccagcaa ccgcgccgcc tgagctgacg ccacagaata ttccttcccg caccgccagt
    34501 tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca gcacctcatc caccagcgca
    34561 gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgcccgg aatactgctg
    34621 ccttcttccg gttgcaggcc aacaatggtc accggtttgg attgttcgcg cataaagcat
    34681 gaaacgccgg tgatagtgcc ggtcgtcccc atgctggaga caaaatgagt gatgcgcccg
    34741 ccggtttgct gccagatttc cggcccggtg gtggtgtaat gcgcataagg gttatcggga
    34801 ttattgaact gatcgagcag ctttccttcg ccacgattcg ccatctccag cgccagatcg
    34861 cgcgcacctt ccatgccctg ctctttggtg acaagaatca gttccgcacc ataagcacgc
    34921 atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat gcgatagcct
    34981 ttcagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc ttcgattaag
    35041 acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga aagtgccgca
    35101 cgatctttca ccgaacctgc cgggttattg ccttccagtt ttaaccacac ttcactgccg
    35161 ttatccggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt ttgttctaat
    35221 gtactcacaa tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg cgtcttatca
    35281 ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc gcctccgggc
    35341 gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct catcgcgggt
    35401 ttcaatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga ataaacgctc
    35461 gccatgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt tgtaccaccc
    35521 cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca gtacctgtac
    35581 cggcagcggc gaatcgagac tggtacggcg gctgatatcc acttcccacg ggcgcaggaa
    35641 gagatccacc ggcccctgat acgcaggtgt gtagcccagc ggccagcgat gcgcgccaac
    35701 atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca taaattcgag
    35761 tacaaaacgg gtcgctggtt cgcgccatac ctgattcggc gcatcagcct gttcaatatt
    35821 cccctgactc atcaccacta cacgatcggc tacttcggtc gcctcttcct ggtcgtgggt
    35881 cacaaacacg ctggtgaatt tcagctcttc atgcagttga cgcagccagc gacgcagctc
    35941 tttacgcacc tgcgcatcca gcgcgccgaa cggttcatca agcagcagaa tttgcggttc
    36001 tacagccagc gcgcgcgcca gcgccacacg ctgtttctgg ccgccggaaa gctgcgccgg
    36061 ataacgatcc gccagatgcg caagctggac catttccagc aattttgtca ctttcgcttt
    36121 gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa aagtgatatt
    36181 gtcgaatacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac cgactttacg
    36241 atcgcgcgcg tgcaggcgac tcacgtcggt gccgtggaag cgaatatgcc cgctggtttg
    36301 atgctccagc ccggcgataa tccgcagcag cgtggttttc ccggaaccgg acggccccag
    36361 caacgcgacc atctgaccag aaggaatatc cagtgagata tcgttcagca cctgggtgcg
    36421 accaaaagac ttcttgatat tggcaatctc aatgctcatg atgctcctcc tgctgcgcgc
    36481 gtttttcctg attctccagg cgccactgca acatactctt taaaaacagg gtgataatcg
    36541 ccatcaacgt caacagcgcg gcggcggtaa aggagccgac ggtgttgtag tcctgctcca
    36601 acaattcaat ctgtaacggc agcgaaagtg tttcgccgcg aatcgagccg gaaaccaccg
    36661 acactgcacc aaactcgcca atcgcgcggg cgttggtcag caccacgccg taaagcagcg
    36721 cccagcgaat attcggcaat gtgacgcgac ggaacatctg ccagccggac gcgccaagca
    36781 aaatcgccgc ttcgtcttcc tggctgccct ggcttaacat caccggcacc agctcgcgca
    36841 ccacaaacgg acacgtcaca aagatggtga ccagcaccat tccgggccag gagaacataa
    36901 tttgcagatt atgttcatcc agccagccgc cgagcgggcc gttggagccg tagaacagca
    36961 gatacaccag accggcaacc accggcgata cagcaaacgg aatgtccagt agcgtcagca
    37021 gtaactggcg tcccgggaag ttaaagcgcg tcaccagcca ggccagcaga atgccgaaca
    37081 ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg gcgtgcagca
    37141 tgtccggatc ggccagattc tgtaaaaccg gcatcagccc cttgctgaat gcctgcacga
    37201 agatgtaaat catcggcacc agcaggatga acgccgaaac cagcatcccg atgccaatca
    37261 gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgg gtaacttccg
    37321 ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa ttgagaacag
    37381 cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgctgccg ggtaatcaaa
    37441 ctcctgtaag cgcacaaaaa tcatcagcga cgtcacttcc gtcttccacg cgatatttcc
    37501 ggcgataaaa atcaccgcgc caaattcacc aagactacgg gtaaacgaca gcgccacgcc
    37561 cgccaccagc gccggagaaa gctccggcag caccactttg cagaaactct gccagcgcgt
    37621 tgcaccaagc gtttccgccg cttcttcata ttccgggcct aactcttcca gcaccggctg
    37681 cacggtacgc accacaaacg gaatgctggt aaaggccatc gccaccgcaa taccaagcca
    37741 ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac cgtttacgga
    37801 aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg gtaaatccat
    37861 cagcgcatca agcagcgtgc ggcccggaaa gcgataacgt gttaaaatcc acgccatcag
    37921 cagaccgaaa acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg tcactttgta
    37981 ggccgcgacc acctgcgggt tggtgatcac ctcccagtac tgcgcccagc tcatctcaga
    38041 aagctgcatc accagcgcag agagcggcag cagcaatatc aggcacacaa acagcagact
    38101 ggtgccaagg cttaaagtaa agcccggcag cacgcgtctt gaagaaactg caaacattac
    38161 ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg ggttttcatc
    38221 acttccggcc atgagccaaa tttgtcttcc acgcggaaca gttcggtctg cgggaattta
    38281 tctttcagtt tgtccatgac ttccgggtta ttcacacggt agtaatagtc ggtgatgatg
    38341 gtctgcgcct gtgggctgta gagccagttc aggtaggctt ttgcggcttt ttccgtaccg
    38401 ttggcctgca catttttatc gacccacgcc accgggaatt ccgccagaat attggttttc
    38461 gggatcacca cttcaaagcc ctgtgcttca tactgtttac ggatgttgtt cacttccgat
    38521 tcaaagctaa tcagcacatc gcccaagcca cgctcggcaa aagtggtggt tgcgccacga
    38581 ccgccggtat cgaacacttc aacgtttttc aggaactggg tcataaactg ttcggttttg
    38641 gctttatcgc caccgtcagc tttatccgct gcgccccatg ccgccagata ggtataacgc
    38701 gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg caccaggtcg
    38761 ttccagtcgt ggatattctt cgggttgccc ttacgcacca ggaagcccat ggtggagtag
    38821 aacggcgagc tattattcgg caggcgcgac tgccagtcag ccgggatcag cttgcctttg
    38881 tcgtgcagga tttgtacgtc ggtcacctgg ttataagtga caacgtctgc ttttaagccc
    38941 tgcaaaatcg ccagcgcctg ttttgatgac ccggcatgag attgttttat cgtcagtttg
    39001 tcgccgccgt tatcttttgc ccattgctgt tcaaacggcg gattcagggc ggcaaacagc
    39061 tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg gcccgccagc
    39121 agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc cattgcgcac
    39181 ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt gaaaggagta
    39241 acggttttat ataccgtttg gtgatttgga agttaaaaag ggaataagaa cttggttcaa
    39301 caggcgtaat tgttgcaggc agtttggaca cggacagcgc ggagaaaccg gagcgtacac
    39361 atagtacgtg aggatttcga gcactgcccg gggccaaaat gacaaatgaa atagcctggt
    39421 gaacttagtt caagacgaaa tcctccccac acatgcaggg aggaaacaga ggtgaatcag
    39481 ataccgacgc taaccgtctc cggcagtgtg ctgccgccat caatcacatt ctgtgtaccg
    39541 gttaaatagc tggattcatc cgatgcgagg aaggccgcca gttcgccgac ttccagcgga
    39601 tcggcgaggc gacacagcgg gattgctttt gccatttcag tcagcaccga ttctggatct
    39661 tccgggttcg actggcgggc aatgctttcc gccattggcg tgcggacgta tcccgggcag
    39721 atggcgttaa cgcgaatacc ggattgcgcg tactccaccg ccagcgattt agtcaggcca
    39781 acaatcgccg ctttcgttaa ggcatacgcc gtttcgccag gatcagccac catatcacca
    39841 gtgactgaag acatcatcac aatgcgacca tctttgcgcg caatcatctc cggcagcacc
    39901 gccttcgtga cgttccatac gcctttaata ttgatatcaa tatggaaatc gcgatcttca
    39961 tcgctcatat cgaggaaact gcccagacga caaacgcctg cgttattcac caggatatca
    40021 atgcgccctt ctttttcctt cgcgcgtttg atagctgcgg ctaccgacgc cgggtcacgc
    40081 acatcggcga caaccgccgt acagcgatga ccacgaccac acagttcgtc cgctagcttt
    40141 tcgatctcag gggagatatc cagcaagatt aggttcgcgc catgacgtgc aaaagttctg
    40201 gcaattcctt cgccaattcc ctgcaatgcg cccgtaatca gtgctgtctt gcccgtgagt
    40261 ttacccattt taatgctctc cttgttaata cagagcgtta cactccattc cctctgaaaa
    40321 tacaggataa acaaaatatt gctgcggatc agctcactaa ttcaggaaaa tctcaatcca
    40381 gtcgctgctt tatgcatcat ttcaggcgtt gtgttagctc actgctgcgc tgaatcattt
    40441 tcaatgacgc cacatcattg atgttgcaca agaccaacaa acagtaaatc ggtaacagaa
    40501 ttttgcgccg tgcgggtaga catcgacgag ctacgccatt cggtttcacc ggaaacggta
    40561 tcgagcgtga aatgcgccag ccgccgcagc ggagagtcag ccagagaagt aatggcaatc
    40621 accgttgccc cctgctttcg tgccgcttct gcgcacaaga caatctcttt tttactgccg
    40681 ctgtaagaga tagcgatttg tacatcgcct tttttcaacg cctgggaaac ggtggcttgt
    40741 acgtgcgtgt cggcttcgca ggccacgcga tagccaattt tcatcagttt gaacgacaga
    40801 tcgcgcccca ccagcgccga tccgcccagg cccgttatct ggataaacgg tgctttactg
    40861 atgacctcaa tgattttttg cagccgcgcg tagtcgaaca acgcacaggt ttgctccagc
    40921 gccagctctt tttcgcgatt cagtttgcgg gcaatcactt ccagcgaatc atcactggta
    40981 atcgaactgt gcaggtgcag cgccgtggca ttcgttttct cccggctggc actgtattcg
    41041 ccaattaacg ccatacgcaa ttcagtaaat ccttgcgcgc cgagcttctg ggcaaatttc
    41101 acaatgctcg actggctgat accgagctgt ttcgccatct gccgtgaaga gacggatttc
    41161 agctcgctca ctctggcccg cagaaagtca gcaatttttt gctcgttccc ggtaaattca
    41221 ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctag gctggcagca
    41281 aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt
    41341 cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa
    41401 atagtaaggt caccaccgat gcaactagaa aagatgatta ctgaaggctc gaacaccgcc
    41461 tcggctgaaa ttgaccgcgt atcgacgctg gaaatgtgcc ggattatcaa cgatgaagat
    41521 aaaaccgtac cgcctgccgt tgatcgcgtc ctgccggata tcgccgcggc gatcgatgtt
    41581 atccacgccc aggtcagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatccggt
    41641 cgtctgggga ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa accgggtctg
    41701 gtggttggtt tgattgctgg aggcgaatat gccattcagc acgcggtgga aggagctgaa
    41761 gatagccggg aaggtggtgt taacgatctg aaaaatatta atttaacggc acaggatgtg
    41821 gtggttggca ttgctgccag cggtcgcacg ccgtatgtga ttgccggact ggaatacgca
    41881 cgccagctcg gctgccgcac agtgggaatt tcctgtaatc cggggagcgc cgtttcaacc
    41941 accgctgagt ttgccattac accgattgta ggtgccgaag ttgttaccgg ttcttcgcgg
    42001 atgaaagcag gtacagcgca gaaactggtg ctcaatatgc tttccaccgg gctgatgatt
    42061 aaatccggca aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg
    42121 catgtgcgcc aggtcaatat cgttaaaaac gccaccggat gtaatgccga acaagcggaa
    42181 gcggcgttaa ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat
    42241 ctcgatgccg cagaagctaa aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt
    42301 ttagacaagg aataacccat ggccaaagag atcagcagtg aacttctgaa caccattctt
    42361 acccgtgtcg gcggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctgcgt
    42421 ctgggtgtac atgacagttc actggttgat cccaatatca aaacgctgga aggcgtgaag
    42481 ggcgtcattt tgaccagcga ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt
    42541 gccgcaaaag cgatgagcga gctgctgggc gatgcaccgg tacaggatgc cgcagaaatc
    42601 gccgcccaga acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc
    42661 gccaaattcg ccaccatctt cacgccgctg atccccggtt ttattgccgc cggtctgctg
    42721 ctggggatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggcacg
    42781 ctacccgacg cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac cttcctagtg
    42841 attctggtgg gctataacgc cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt
    42901 attgccgcac tctttttgct cggttataac cccgctgcaa ccaccggtta ctacgccggt
    42961 tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgctgatt
    43021 gccgcgtggg cctgcgcccg cattgaaggc atggtgcgcc gctttatgcc ggacgatctc
    43081 gacatgctgc tgacctcgtt aatcaccctg ctgatcaccg ctacgcttgc gtacctgatc
    43141 attatgccgc tgggtggctg gttattcgaa ggtatgtcat ggctgtttat gcacctgaac
    43201 agtaatccgt tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt ggtatttggc
    43261 gtgcatcagg gctttattcc tgtttacctc gcgttaatgg acagccaggg attcaacagc
    43321 ttatttccca tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac
    43381 tggcgggcgc aaccgcacag tgcgctacgc agtcaggtac gcggggcgat tattcccggc
    43441 ctgctgggcg ttggtgaacc gctgatttac ggtgtcaccc tgccccgcat gaagccgttt
    43501 gttaccgcct gtttaggcgg cgcggcgggc ggtttgttta tcggattgat agcctggtgg
    43561 ggtctgccga tgggcttaaa cagcgccttt gggccgtctg gtctggtggc gctgccgctg
    43621 atgacttccg cacaaggcat ccttccggca atggcggttt atgctggcgg gattctggtg
    43681 gcatgggttt gcgggtttat tttcaccacg ctctttggct gccgtaacgt caatctggac
    43741 tgatattatg aaacggacaa tgctctatct ttctctgctg gctgttagtt gtagcgtcag
    43801 cgccgcaaaa taccctgttc tgacagaagg tagttgatca agtatatgcg gcgttaaagc
    43861 agtaataaaa aggccagtca gtaatgttac tgcctggcct ttaatcaatg aatcagaaac
    43921 gcttacagcg ccatcaactt gtccagcgac ggtgcgaaat aatagccgcc ggttaccggt
    43981 ttggtgaaac gcaacatcgc atcacgtttg ccatcggtat cgccaaacat gctcagcagt
    44041 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    44101 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    44161 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    44221 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    44281 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggaatc cacgccgtct
    44341 ttgataaccg ccacttcgcg gcgcgtctct tcacccgccg ggttttctgt accgtcaaca
    44401 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    44461 atgcagtcac caaaggcttc catcgccgcc tgagcgacag agaagtttac gtcgtgacgc
    44521 agagaaagaa tgtggatcaa cacatcgaac tgggtcgtcg gcgcaaggcc tttaccgtag
    44581 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    44641 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    44701 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    44761 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    44821 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    44881 cgtgtttttt cgcgacggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    44941 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    45001 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    45061 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    45121 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    45181 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    45241 aaaagcgcac cacgttgccg ttcgtggttt tcattccgct gcgcaggcga taatcgccat
    45301 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    45361 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gcagaccagt
    45421 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    45481 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    45541 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    45601 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    45661 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    45721 acagaaaata gagcacatgc caggtgcgag gaaacgaagc ccgccgaccg ctcaacaaaa
    45781 acgccagcac cgccggaata ccgggaatca gccccagcca gaaattatcg tgatcgggat
    45841 aaaacagatt tagcagcgca gtaccctgct cacgcgacgc accggcaatg acaaacagca
    45901 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    45961 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    46021 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    46081 tcgttatctt ccggcacgtt gatctgaatt ttcgggcagc cgcgagcaat cagctttttc
    46141 tccagccggt taagcaacgc attggcaatt ccacgcccac gaaactctgg atgcacgcca
    46201 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    46261 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    46321 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    46381 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    46441 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    46501 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacag
    46561 caaaccgtcg taacggatta cgcgatacga tataacatct gaaactttat tattacaact
    46621 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    46681 aagccggttt agctgccctg acgttgtcag gaatgtcgca agccattgcc aaagacgaac
    46741 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    46801 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    46861 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    46921 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    46981 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    47041 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    47101 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    47161 cgactgacaa ggatcaccta ttgcagcaag tactgtttga tctggtgcaa accgatacta
    47221 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    47281 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    47341 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    47401 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    47461 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    47521 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    47581 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgcagtc
    47641 gggtgttgcg taatggtggt gttttcgaac aggcgggcgt caacttttct cacgtccacg
    47701 gtgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    47761 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    47821 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtttgg tggtttggcg
    47881 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    47941 ccgcccgtga cctgtgccag ccatttggtg aagacgttta tccccgttac aaaaagtggt
    48001 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggcattggc gggctgttct
    48061 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    48121 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    48181 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    48241 gggatcgcgg cacgctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    48301 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    48361 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    48421 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    48481 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    48541 gcgaccacgg gcttatcagt tcgcggcgta cgacgttcag gcgaatgcgt ttcagccacg
    48601 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    48661 gcagttgatt acacaaatcc agcaccgtca ccggttcgga catgttttcc agcacatatt
    48721 cacgggcgcg ggaaagcaac cgacggtaac tctgatgact gatgctttcc gccgtcacca
    48781 ttggttgcgc ttcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    48841 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    48901 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    48961 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacag gtgatgacat
    49021 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    49081 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    49141 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    49201 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagt agaatttccg
    49261 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc taggcggtaa
    49321 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    49381 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    49441 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    49501 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    49561 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcggccactt
    49621 ctcctgccgt cattccctga catactgatg ttaacagcgc caacaactcg tcagcagatt
    49681 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    49741 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttacgg ctgatgacac
    49801 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    49861 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    49921 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    49981 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    50041 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    50101 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggacg taggtcacca
    50161 gctgtacgtc ggcagatttc aacgcggcat caatgccgta ggtcgcttcc agcggcggtg
    50221 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    50281 cggtacgcga aaccacatgc gccaggaagg cggtgttttc tgcgtcattg gcccactgga
    50341 aagctgcgcc attttcaata tgtgcaacca tcgcatccag accggcacgc acttccgccg
    50401 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggg ccgtgtgccg
    50461 ctcccgcata cagcgagcga ccatatacca cttcgaccat cgcttgtttg gtcgcttcgt
    50521 cagccgcaat gtaggttacg tcgtcagaat cggcagaaat cagcccgagg ctacgaatat
    50581 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg
    50641 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    50701 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggcacagc
    50761 agcacgaaag agaagcaaaa gtcagctacc tggtacacta ggcagcgggt cagcaaaggg
    50821 gggcaaatgc cccttttttc atgtcagata atcagatcat tacctcaata aactaataat
    50881 cacatcgcgg ttacaaccag tttgatagtg atagctcaaa cccagacgtg gcgctggctg
    50941 caagcaaaat aagccgcaaa aatgcgaccc catgtttact agccgcaaaa atgcggtcct
    51001 atttgccaga accttttatt attcaattgg ttagtgcaag aaaaagcgtg attactgttc
    51061 aagaagtatc cagaaaggtc atgattttga cgctgaaaaa aaataggtgg gcatttttgc
    51121 ccactcacta gccgcaaatt tgcccactca ctagccgcat ttttgcggcc caaagtaata
    51181 aatacagaga aataataaat acagatcagc gccaagattt ttcgtctttt cggcggaatc
    51241 atgagactag tgcttacgtc ctgataacct ccagagcaga ccttaacttc tgtgcagcag
    51301 gactacgctc attgccgctc ggctaggtca aatcctcacg ataaagctgt tcggattgtg
    51361 accataatgt taaaggactc tttaaatcat tccttgacga attaactagc tggcttaagc
    51421 ggaatagcat gcaggataaa aacaaaacca gcttagcagt ggggagagct tcgcttcgct
    51481 cgtctcctgg ggccaatgct caatgaaacg acgtaaggca cggagcgctc accataggta
    51541 gacacataaa catctgagag cgcaccttct atcgcgctac gcgctcaata aaaaccaacg
    51601 acaccgccac actgcaagac caatggtttt tatcgcgtat acagaccaga caggaaacag
    51661 cagcaaagca gcgtatagcc aggtgcagac agcacactca cacgacgcac agcaggctta
    51721 agtgatcact caggaaactt tatccccgcc gccgatcacc gcgaactatc atgaccgcgc
    51781 caaattcgaa aggaatggcc catagaacgc gatgaacaca cataagcgct acacttactt
    51841 aacgtcgcca cagagcgatt gagagcgctt aaaatcgatg cgatttcatt aaatttttct
    51901 cactcgcctg aatggtggcg atcaggactg atcgcaacat tgccccgcct cgatattgag
    51961 cgcataatag atacatacat taagcggaaa agagataagt ttattgtgaa aatcaggggg
    52021 ttacatatca tgcgcatatc agaacaacaa cggcagatcc tcgactatct ggcaaaaaac
    52081 gaatcaatca ccgcacgaca agccgcagat ctcgtttatg gtggcaatgt tacccgcgtc
    52141 caggttgacg ccgcccgccg ctcactcatg acgatggtta gtaatgggct aatcaggaaa
    52201 caaggccgcg ctttcgtagc tgaaagacat atcaatcacg cttatagcca ggacgttgaa
    52261 gaattgcgcc gccagatcct ttgggtattc agcaaacagg gtcaggagta tttcctttct
    52321 gaacacccaa atttcgccgc caaagagtta acagtaagcg gattgtcgtg cgcactggat
    52381 tatcttgtcg gtgaaggttg cgttacttac aagcagctat ggcacgccct gaatacgctt
    52441 gtagaagatg gtttactcat tgccacgcgt gagaacatcg aatactggat aatgccgcgt
    52501 tcaggtcgcc gcaatcatgc acggtatatt tcaggcaaca gatacagaac gccggacttt
    52561 aatccgacac aggaagatcg ggaccgtgcc gaaatgatgt gcagggaaca ggagaaaaga
    52621 attatggata agttattttc gatgcagtga acacaagcgg gcgcaatgcc cgtttttatt
    52681 gccttattcc acgcctgacg aatcaatttg atcacacctg agaaaatgca tagccagaag
    52741 ttaaggccgt tatattgcga ttgggagcgt tttaaacggt atcaatttaa ttaattctga
    52801 tgtggtgatt tgcaggcaaa aaaaacccgc cggagaagcg ggaaatagag acatatcgtt
    52861 gtagttatta acagatcact ttgaggtcga gggcggctaa ccctcgctaa tattatcccg
    52921 ctattacctt gcgggtgcaa ataatctttt attgctttga tgatttaata aatagcactt
    52981 tttgctaaca aaaaccgctg tcaatatatg gccaaaaatg tcaacagaaa aaataaggtt
    53041 tataatatta agtatcacat gggtaatggg ttcagggaat cggaatccac cgcctcacgg
    53101 aaattcaaaa tttgcttctt ctttcctccg tgggcggctt ctttttcgta tcgaggtatt
    53161 tatgaaagca ataacattag aacagttcgc agcaatggcg aatgtatcga ccagaacgct
    53221 gaggcgctgg ctaaaaaaac catcaaccgg agtccctttg ccatttaccc cacgcggtac
    53281 tcgccaatta ttctgggagc acgaatgcaa agaatggttg tacagaacag aacgataata
    53341 atcacgcatc acattttgag gtaaacatca tgaccaatat cgaaagatta gaacgctgtg
    53401 ccgatattat gcgacgcaga tggatttatg accctgaagc gggtttattg tttagccgtg
    53461 aaacaaaaga cgttatcaaa ggcagcatga ccaataaagg gcatttggtt gtaacggtac
    53521 atgaaggtaa attcaaagtg acactcacct atcagaaagc ctgctatgta tacacatacg
    53581 gcgcttatga cgagacatta tatgaagtgc accacgttaa tttgaacaaa caggataacc
    53641 gcatcaagaa tattcgcctg atgccgaaag aaaagcaccg ccggatacat aaaaacgtcc
    53701 gcaaacttat tcggctgggc cacacacaat ggcttcaacc gcaattaatt gaacaatcac
    53761 tataaacaca ggagtaaata ccatgcattc attagacagt tattttcagc gcacgacagc
    53821 ccctaaatca gccgcccagg aacgccgtga ggaattccat gagaaagtga tgcgttctgc
    53881 tgattatatt gcggacaagt tcgtggaaac ggttcgtcca cttgttgatg aagtagctga
    53941 taaacttcag tctgaaatgc cggaagatat ggaaggcact gcaaagcggc gactcatttg
    54001 cgaattaagt cgtcgttttg gcgtgtcaat aagcgctttt aaataatcga aaaaaggggg
    54061 gaaagataat ggacgcattt gaggtactgg caaataaagg cgctgaactg gtagcacagc
    54121 gtgataaaac cgccaacgaa ggggaacgcg cactgctaaa taaacagatt aaagcgatcc
    54181 gtatggccca gtttaaatta atcagtaatg aagttatcga aacaattaaa ccgcagatcc
    54241 cacaaatcgt tgctgacgct attaaagccg ccggactgga gaaacgcatt gccgggctaa
    54301 aaacgaacgc agagaaaatg gagatgtaca aacagagcgg tgctccgaat cccgatgagt
    54361 acttctcacc ggatgttgag tttatggcgc aggttgagga acgtctgggt agctgtctca
    54421 ccgaggaaca gcgtcgctat tttgatggag tggatagttc ggcaggtatt gacctgaact
    54481 cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc gtctatgccg gagccagaac
    54541 cagaaatggc caccgatcct gaagctgagc aacggaaacg cgtattcaat gcgataggga
    54601 ttcggtactg atcggataaa agaaagccgg gaattacccc ggctgactta acaatgtgac
    54661 caatgaacaa atcggacaag acaaacagga taagcagtct cgaaacaatg ttcgggaact
    54721 atattagcac aagtccggag aaaatcatca tgttggcact acgcatcatg cagggcatcg
    54781 ctaagaccct cgcagaacat gtcttagacc tgaaacattc gccactgagc aaacaggcca
    54841 tgaagcgcca aacgcttcgt ctgtgggctg aatattcgct tggaactatc aataaaatca
    54901 tcgacatgaa atcggggccg agcaaccaga gcgcggagga aatggagttc atcaggcgtt
    54961 taattctcat tcgcagagac attcatagcc agttacacag cgtgggaatc gatattaatg
    55021 atgggacggg cgattaattt tcggacaagt actatgcgcg cgcgaggagc tgaacatgaa
    55081 gcaagatatt gcagacaggc tggagatact cgaaggccag cgggcagaag cgaaacaact
    55141 acgtaagcag gccagaagag cgcacaggaa caacgaagca gagttactaa ccaaatacat
    55201 cagtttcacc aattactgca tctacgagtg ttacaaggaa gatgcagagg actggctgga
    55261 ttcattgcca gaacagtact gagcagaata acaccacgtc atgttgttct gaggtgagca
    55321 tatcacctct gttcaggtgg ccaaatttag tgtgccactt cagcatcacc gttgaaatca
    55381 caataacgat ttttttgtaa atatgctctc acatatgtag gaatagacca ggttggcatt
    55441 gaaacacatc taccatgacc ataataaa
//
LOCUS       DAZPDE010000005        55418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPDE010000005 DAZPDE010000000
VERSION     DAZPDE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.005"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHU0J_000263"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHU0J_000263"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1215120.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHU0J_000264"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHU0J_000264"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1215121.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHU0J_000265"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHU0J_000265"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1215122.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHU0J_000266"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHU0J_000266"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1215123.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHU0J_000267"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHU0J_000267"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1215124.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHU0J_000268"
     CDS             7826..9559
                     /locus_tag="ACHU0J_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1215125.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHU0J_000269"
     CDS             9708..10559
                     /locus_tag="ACHU0J_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1215126.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHU0J_000270"
     CDS             complement(10546..10887)
                     /locus_tag="ACHU0J_000270"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1215127.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHU0J_000271"
     CDS             complement(10889..11767)
                     /locus_tag="ACHU0J_000271"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1215128.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHU0J_000272"
     CDS             complement(11733..14030)
                     /locus_tag="ACHU0J_000272"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1215129.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHU0J_000273"
     CDS             complement(14081..14401)
                     /locus_tag="ACHU0J_000273"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1215130.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHU0J_000274"
     CDS             complement(14416..15495)
                     /locus_tag="ACHU0J_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1215131.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHU0J_000275"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHU0J_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHU0J_000276"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHU0J_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHU0J_000277"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHU0J_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHU0J_000278"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHU0J_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHU0J_000279"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHU0J_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1215132.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHU0J_000280"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHU0J_000280"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1215133.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHU0J_000281"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHU0J_000281"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1215134.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHU0J_000282"
     CDS             25474..25608
                     /locus_tag="ACHU0J_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215135.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHU0J_000283"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHU0J_000283"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1215136.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHU0J_000284"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHU0J_000284"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1215137.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHU0J_000285"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHU0J_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1215138.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHU0J_000286"
     CDS             30120..30728
                     /locus_tag="ACHU0J_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1215139.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHU0J_000287"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHU0J_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1215140.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHU0J_000288"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHU0J_000288"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1215141.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHU0J_000289"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHU0J_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1215142.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHU0J_000290"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHU0J_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1215143.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHU0J_000291"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHU0J_000291"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1215144.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHU0J_000292"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHU0J_000292"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1215145.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHU0J_000293"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHU0J_000293"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1215146.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHU0J_000294"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHU0J_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1215147.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHU0J_000295"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHU0J_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1215148.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHU0J_000296"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHU0J_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHU0J_000297"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHU0J_000297"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1215149.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHU0J_000298"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHU0J_000298"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1215150.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHU0J_000299"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHU0J_000299"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1215151.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHU0J_000300"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHU0J_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1215152.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHU0J_000301"
     CDS             complement(44843..45142)
                     /locus_tag="ACHU0J_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1215153.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHU0J_000302"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHU0J_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHU0J_000303"
     CDS             45895..46494
                     /locus_tag="ACHU0J_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1215154.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHU0J_000304"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHU0J_000304"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1215155.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHU0J_000305"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHU0J_000305"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1215156.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHU0J_000306"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHU0J_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1215157.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHU0J_000307"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHU0J_000307"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1215158.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHU0J_000308"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHU0J_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1215159.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHU0J_000309"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHU0J_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1215160.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHU0J_000310"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHU0J_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1215161.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHU0J_000311"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHU0J_000311"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1215162.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHU0J_000312"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHU0J_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1215163.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZPDE010000006        54186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPDE010000006 DAZPDE010000000
VERSION     DAZPDE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.006"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..698
                     /gene="lpxT"
                     /locus_tag="ACHU0J_000313"
     CDS             <1..698
                     /gene="lpxT"
                     /locus_tag="ACHU0J_000313"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1215164.1"
                     /translation="QIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQKLVESK
                     AFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTAVVLNQL
                     GQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAFMWRYFG
                     KVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDRLITFFD
                     KSLPGKNKHFQNK"
     gene            1110..1676
                     /gene="mepS"
                     /locus_tag="ACHU0J_000314"
     CDS             1110..1676
                     /gene="mepS"
                     /locus_tag="ACHU0J_000314"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1215165.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1857..3413
                     /locus_tag="ACHU0J_000315"
     CDS             1857..3413
                     /locus_tag="ACHU0J_000315"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1215166.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            3495..5309
                     /locus_tag="ACHU0J_000316"
     CDS             3495..5309
                     /locus_tag="ACHU0J_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1215167.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            5310..6404
                     /locus_tag="ACHU0J_000317"
     CDS             5310..6404
                     /locus_tag="ACHU0J_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1215168.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6404..7429
                     /locus_tag="ACHU0J_000318"
     CDS             6404..7429
                     /locus_tag="ACHU0J_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1215169.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7431..9020
                     /gene="yejF"
                     /locus_tag="ACHU0J_000319"
     CDS             7431..9020
                     /gene="yejF"
                     /locus_tag="ACHU0J_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1215170.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(9024..9368)
                     /locus_tag="ACHU0J_000320"
     CDS             complement(9024..9368)
                     /locus_tag="ACHU0J_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1215171.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9701..10897)
                     /gene="bcr"
                     /locus_tag="ACHU0J_000321"
     CDS             complement(9701..10897)
                     /gene="bcr"
                     /locus_tag="ACHU0J_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR1215172.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(10925..11620)
                     /gene="rsuA"
                     /locus_tag="ACHU0J_000322"
     CDS             complement(10925..11620)
                     /gene="rsuA"
                     /locus_tag="ACHU0J_000322"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1215173.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11770..13530
                     /locus_tag="ACHU0J_000323"
     CDS             11770..13530
                     /locus_tag="ACHU0J_000323"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1215174.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13655..13939
                     /gene="rplY"
                     /locus_tag="ACHU0J_000324"
     CDS             13655..13939
                     /gene="rplY"
                     /locus_tag="ACHU0J_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1215175.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(14078..15085)
                     /gene="yejK"
                     /locus_tag="ACHU0J_000325"
     CDS             complement(14078..15085)
                     /gene="yejK"
                     /locus_tag="ACHU0J_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1215176.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15267..15494
                     /locus_tag="ACHU0J_000326"
     CDS             15267..15494
                     /locus_tag="ACHU0J_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1215177.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            15514..17274
                     /gene="yejM"
                     /locus_tag="ACHU0J_000327"
     CDS             15514..17274
                     /gene="yejM"
                     /locus_tag="ACHU0J_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1215178.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            17349..17425
                     /locus_tag="ACHU0J_000328"
     tRNA            17349..17425
                     /locus_tag="ACHU0J_000328"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17383..17385,aa:Pro,seq:ggg)
     gene            complement(17528..20119)
                     /gene="yejO"
                     /locus_tag="ACHU0J_000329"
     CDS             complement(17528..20119)
                     /gene="yejO"
                     /locus_tag="ACHU0J_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR1215179.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            20439..21086
                     /gene="narP"
                     /locus_tag="ACHU0J_000330"
     CDS             20439..21086
                     /gene="narP"
                     /locus_tag="ACHU0J_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1215180.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(21297..22349)
                     /gene="ccmH"
                     /locus_tag="ACHU0J_000331"
     CDS             complement(21297..22349)
                     /gene="ccmH"
                     /locus_tag="ACHU0J_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1215181.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(22346..22903)
                     /gene="dsbE"
                     /locus_tag="ACHU0J_000332"
     CDS             complement(22346..22903)
                     /gene="dsbE"
                     /locus_tag="ACHU0J_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1215182.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(22900..24843)
                     /gene="ccmF"
                     /locus_tag="ACHU0J_000333"
     CDS             complement(22900..24843)
                     /gene="ccmF"
                     /locus_tag="ACHU0J_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1215183.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(24840..25319)
                     /gene="ccmE"
                     /locus_tag="ACHU0J_000334"
     CDS             complement(24840..25319)
                     /gene="ccmE"
                     /locus_tag="ACHU0J_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1215184.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(25316..25525)
                     /gene="ccmD"
                     /locus_tag="ACHU0J_000335"
     CDS             complement(25316..25525)
                     /gene="ccmD"
                     /locus_tag="ACHU0J_000335"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1215185.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(25522..26259)
                     /gene="ccmC"
                     /locus_tag="ACHU0J_000336"
     CDS             complement(25522..26259)
                     /gene="ccmC"
                     /locus_tag="ACHU0J_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1215186.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(26301..26963)
                     /gene="ccmB"
                     /locus_tag="ACHU0J_000337"
     CDS             complement(26301..26963)
                     /gene="ccmB"
                     /locus_tag="ACHU0J_000337"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1215187.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(26960..27583)
                     /gene="ccmA"
                     /locus_tag="ACHU0J_000338"
     CDS             complement(26960..27583)
                     /gene="ccmA"
                     /locus_tag="ACHU0J_000338"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1215188.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(27596..28198)
                     /gene="napC"
                     /locus_tag="ACHU0J_000339"
     CDS             complement(27596..28198)
                     /gene="napC"
                     /locus_tag="ACHU0J_000339"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1215189.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(28208..28657)
                     /gene="napB"
                     /locus_tag="ACHU0J_000340"
     CDS             complement(28208..28657)
                     /gene="napB"
                     /locus_tag="ACHU0J_000340"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1215190.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(28654..29517)
                     /gene="napH"
                     /locus_tag="ACHU0J_000341"
     CDS             complement(28654..29517)
                     /gene="napH"
                     /locus_tag="ACHU0J_000341"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1215191.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(29504..30199)
                     /gene="napG"
                     /locus_tag="ACHU0J_000342"
     CDS             complement(29504..30199)
                     /gene="napG"
                     /locus_tag="ACHU0J_000342"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1215192.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(30206..32692)
                     /gene="napA"
                     /locus_tag="ACHU0J_000343"
     CDS             complement(30206..32692)
                     /gene="napA"
                     /locus_tag="ACHU0J_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1215193.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(32689..32952)
                     /gene="napD"
                     /locus_tag="ACHU0J_000344"
     CDS             complement(32689..32952)
                     /gene="napD"
                     /locus_tag="ACHU0J_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1215194.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(32942..33436)
                     /gene="napF"
                     /locus_tag="ACHU0J_000345"
     CDS             complement(32942..33436)
                     /gene="napF"
                     /locus_tag="ACHU0J_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1215195.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            33545..33709
                     /gene="yojO"
                     /locus_tag="ACHU0J_000346"
     CDS             33545..33709
                     /gene="yojO"
                     /locus_tag="ACHU0J_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1215196.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            33823..34332
                     /gene="eco"
                     /locus_tag="ACHU0J_000347"
     CDS             33823..34332
                     /gene="eco"
                     /locus_tag="ACHU0J_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1215197.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(34454..36078)
                     /gene="mqo"
                     /locus_tag="ACHU0J_000348"
                     /pseudo
     CDS             complement(34454..36078)
                     /gene="mqo"
                     /locus_tag="ACHU0J_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(36296..37939)
                     /gene="yojI"
                     /locus_tag="ACHU0J_000349"
     CDS             complement(36296..37939)
                     /gene="yojI"
                     /locus_tag="ACHU0J_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1215198.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(38015..38665)
                     /gene="alkB"
                     /locus_tag="ACHU0J_000350"
     CDS             complement(38015..38665)
                     /gene="alkB"
                     /locus_tag="ACHU0J_000350"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1215199.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(38665..39729)
                     /gene="ada"
                     /locus_tag="ACHU0J_000351"
     CDS             complement(38665..39729)
                     /gene="ada"
                     /locus_tag="ACHU0J_000351"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1215200.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(39803..40858)
                     /gene="apbE"
                     /locus_tag="ACHU0J_000352"
     CDS             complement(39803..40858)
                     /gene="apbE"
                     /locus_tag="ACHU0J_000352"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1215201.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(40970..42091)
                     /gene="ompC"
                     /locus_tag="ACHU0J_000353"
     CDS             complement(40970..42091)
                     /gene="ompC"
                     /locus_tag="ACHU0J_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1215202.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            42829..45501
                     /gene="rcsD"
                     /locus_tag="ACHU0J_000354"
     CDS             42829..45501
                     /gene="rcsD"
                     /locus_tag="ACHU0J_000354"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1215203.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            45518..46168
                     /gene="rcsB"
                     /locus_tag="ACHU0J_000355"
     CDS             45518..46168
                     /gene="rcsB"
                     /locus_tag="ACHU0J_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1215204.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(46368..49217)
                     /gene="rcsC"
                     /locus_tag="ACHU0J_000356"
     CDS             complement(46368..49217)
                     /gene="rcsC"
                     /locus_tag="ACHU0J_000356"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1215205.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(49492..50268)
                     /locus_tag="ACHU0J_000357"
     CDS             complement(49492..50268)
                     /locus_tag="ACHU0J_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1215206.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(50273..51922)
                     /locus_tag="ACHU0J_000358"
     CDS             complement(50273..51922)
                     /locus_tag="ACHU0J_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1215207.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(51923..54186)
                     /locus_tag="ACHU0J_000359"
                     /pseudo
     CDS             complement(51923..54186)
                     /locus_tag="ACHU0J_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        13243 a        14686 c        13269 g        12988 t
ORIGIN      
        1 cgcaaatagt gttgttgaat attgtcggcc ccgcgctgtt tctttcctgg tatattcccg
       61 ttaatcatgg attctggttg ccgattgatg cggatatttt ttatttcttt aatcagaaac
      121 tggtcgaaag taaggccttt ttatggctgg ttgcattgac taacaatcgc gccttcgacg
      181 gttgttcact gctggcgatg ggtatgttga tgctgagttt ctggctgaaa gaaaacgccc
      241 ctggcagacg acgtatcgtg attatgggtc tggtcatgct attaactgca gtggtattaa
      301 accagctggg tcaggcatta attcctgtaa aacgggccag cccaacattg acttttaccg
      361 atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca cgagatagct
      421 ttcccggcga tcacggcatg atgctgctta ttttttcggc attcatgtgg cgttatttcg
      481 gcaaagttgc aggccttatc gcccttatta tttttatggt ttttgcattt cccagagtaa
      541 tgattggcgc acactggttt actgacatca ttgtcggttc gatgaccgtg atattgatcg
      601 gtttgccctg ggtgttgctg acgccattaa gtgatcgatt aatcaccttt tttgacaaat
      661 cactaccagg aaaaaacaaa catttccaaa acaaataact cacagtaatt aacatcatca
      721 gggttatttt tatagtgagg ataatcctga tgatgcgcac cgtgctttca tctatcgaac
      781 gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg tttcactcaa ttgtacttta
      841 attgaccaac cccgcttatt aactttctgt atcacttttt cttataaaaa atcatgtaaa
      901 accgctcgcc aagaccgcac caatcgggta atctcgaact cgttttgcct cggcggtaga
      961 ttatcctcac agcatataat tttgtgcgtt agtccacaga tttggcctta aggaattgtt
     1021 tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt tttttataac gatatttgtc
     1081 gttaaggact tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata
     1141 tcttgcgcgg gattcccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata
     1201 acaccgcaaa gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc
     1261 aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta
     1321 tggatcagta tgctgactgg aaaggcgtac gttatcgtct gggcggcagc actaaaaaag
     1381 gtatcgattg ttctggtttc gtacagcgta cattccgtga gcaatttggc ttagaacttc
     1441 cgcgttcgac ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta
     1501 cgggtgattt agttctgttc cgtgccggtt caacgggacg ccatgtcggt atttatatcg
     1561 gcaacaatca gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg
     1621 aaccgtactg gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa
     1681 ccgtttggat gcaatccctt ggctatcctg acgagttaac tgaaagcact gcttaggcag
     1741 tgcttttttg ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata
     1801 gtccggtcat tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt
     1861 tcatacgcgc tcccaattct ggacgtaagc tcctgcttac ctgcattgta gcaggcgtga
     1921 tgattgcgat actggttagc tgccttcagt ttttagtggc ctggcataag cacgaagtca
     1981 aatacgacac actgattatc gacgtacaaa agtatctcga tacctatttt gccgacctga
     2041 aatccactac tgaccggctt cagccgctga ccttagatac ctgccagcag gctaaccccg
     2101 aactgaccgc tcgcgcggcg tttagcatga atgtccgtac gtttgtgctg gtgaaagata
     2161 aaaaaacatt ctgttcatct gcgactggtg agatggacat tccactcaat gaattgattc
     2221 cggcgctcga cattaataaa aacgtcgata tggcgatctt acccggcacg ccgatggtgc
     2281 cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat agcggtgtgt
     2341 ttgccgctct gaatctcaac ctgacgcctt ctctctttta tagttcacgg caggaagatt
     2401 acgatggcgt cgccctcatt attggcaata ctgcgctatc taccttttct tcacgtttga
     2461 tgaacgttaa tgaattaacc gacatgccgg tccatgaaac taaaattgcg ggcattcctc
     2521 tgaccgttcg gctttatgcg gatgactgga catggaacga tgtgtggtac gcatttttac
     2581 tgggcggcat gagtggaact gtcgttggcc tgctttgcta ttacctgatg agcgtacgta
     2641 tgcgccccga cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggcgtatc
     2701 aaccggtggt ggatacacaa gctttgcggg taacgggcct ggaagtactg ctacgctggc
     2761 ggcatcctgt agcgggagaa attcccccgg atgccttcat taactttgcc gaatcgcaaa
     2821 agatgattgt gccgctgact cagcacctgt ttgagttaat tgcccgcgat gccgcagaat
     2881 tagaaaaagt actgccggta ggcgtcaaat ttggcattaa cattgcgccg gaccatctgc
     2941 acagcgaaag ctttaaagca gatatccaga aactgctcac ttccctgccc gcacaccatt
     3001 tccagattgt gctggaaatt accgagcgcg atatgctgaa agagcaagaa gccacacaac
     3061 tcttcgcctg gctgcactcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc
     3121 acagcgcgct tatctatctt gagcgtttta cgctcgatta tctgaaaatt gaccgtggat
     3181 ttatcaacgc catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc
     3241 tggcgaaacg tctcaatatg ctgacagtta ctgaaggggt cgaaacgccg gaacaagcgc
     3301 gatggctaag cgaacgcggc gttaatttca tgcaaggcta ctggattagt cacccgttac
     3361 cgctggacga ttttgttcgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc
     3421 ttacgtccct tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag
     3481 gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcaccttcg
     3541 gtgcgcaggc gcaggctatc aaggaaagct atacctttgc cgtgctgggc gaaccccggt
     3601 acgcgtttaa tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga
     3661 taacgttgtc agccctcggt accttcgata atttcaaccg ctatgcactg cgcggcaacc
     3721 cgggcgcacg caccgagcag ctgtacgaca cgttatttac gacttccgat gacgaaccag
     3781 gcagttatta cccgctgatt gctgaaagtg cacgctatgc tgacgattat tcctgggtgg
     3841 aggtcgctat taatccgcgc gcccgttttc atgatggttc tcccattact gcccgcgatg
     3901 tagagtttac ttttcaaaaa tttctgaccg aaggcgtgcc gcaatttcgt ctggtctaca
     3961 aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg cattgagtta gctaaacccg
     4021 gcaaagaaga tatgttgagt ctgttttcgc tgccggtatt tccagaaaag tactggaagg
     4081 atcacaaact tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg taccacgtta
     4141 cgtcctggaa aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa
     4201 acttaccggt aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg
     4261 atgataatgt cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg
     4321 acgctaaaaa ctgggccacg cgttataccg gtaaaaattt cgataaaaaa tacatcatca
     4381 aagatgagca aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac
     4441 gtgcggtatt cagcgatcgc cgggtgcggg aagcaatcac tctcgccttt gactttgaat
     4501 ggatgaacaa ggcgttgttt tacaacgcct ggagtcgcac gaacagttat tttcaaaata
     4561 ccgaatacgc ggccagaaat taccccgacg ccgcggagct ggtgcttctg gcaccaatga
     4621 aaaaagatct accgccagaa gtcttcacac aaatctacca gccgccggta tctaaaggcg
     4681 atggctacga tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa gcgggctggg
     4741 tgctgaaggg tcagcaacgc gttaatgcca caacgggtca gccgctcagc tttgaattat
     4801 tgcttcccgc aagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc
     4861 tgggtatcaa tatggacatt cgcaaggtgg ataactcgca aatcactaac cgcatgcgca
     4921 atcgcgacta tgacatgatg ccgcgactat ggcgggcgat gccgtggccc agttccgatt
     4981 tacagatttc ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa
     5041 gcccggttat cgactcactg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat
     5101 tactgccgtt ggggcgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa
     5161 tgtggtacat ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtac
     5221 gccctgttta cagcctgggt atcgatacct ggtggtatga cgttaataaa gcggccaaac
     5281 tgccgtcagc cagacaacag ggagagtaga tgggcgctta cctgattcgc cgtctgttgc
     5341 tggtgatccc aacgttatgg gctattatca ctatcaactt tttcatcgtg caaattgcgc
     5401 ctggcggtcc ggtcgatcag gccatcgccg ccattgagtt tggtaatgcc ggagtattac
     5461 ccggcgcagg cggtgaaggt gttcgtgcca gccatgcgca aacgggtgtc ggcaatatca
     5521 gcgacagtaa ttaccgtggc ggacgcggat tagatccaga agtgatcgct gagatcactc
     5581 atcgctacgg tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca
     5641 tccgctttga ttttggtgat agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag
     5701 acagtctgcc ggtttccatc accctcggat tgtggagcac gctgattatc tatctggtgt
     5761 cgattccgtt aggcattcgc aaagctgttt ataatgggag tcgctttgac gtctggagta
     5821 gcgcatttat catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg
     5881 tcttcttcgc gggcggcagc tatttcgacc tgttccctct acgcggcctg gtttccgcta
     5941 actttgattc gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctac
     6001 cggtgctggc gacagtgatt ggtggctttg cggcgctgac catgctgacc aaaaactcat
     6061 tccttgatga agtgcgcaag caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaga
     6121 aaaacattct ctggaaacat atgttccgca acgccatgct gctggtgatt gccggttttc
     6181 cggcgacgtt tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac
     6241 tcaatggtct tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt
     6301 ttggtacctt gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca
     6361 gctatacgct ggttgatccg cgtatagatt ttgagggacg ttaatgtcgc gactcagccc
     6421 cgtcaatcag gcccgttggg cgcgttttcg tcataaccgt cgcggctact ggtcgttatg
     6481 gattttcctc gttttgtttg gtttgagttt gtgttctgaa cttatcgcta acgataaacc
     6541 gttgctggtg cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag
     6601 cgattttggc ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg
     6661 gctggaaaat aacggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa
     6721 ctttgctacc gataagccct tcccttctcc accctcccgg caaaactggc tgggaacgga
     6781 tgcaaacggc ggcgatgtgc tggcacgcat tctctatggc acgcggatct cggttctgtt
     6841 tggcctgatg ctgactctct gttccagcgt gatgggcgtg ctggcggggg cgctacaagg
     6901 ctattacggc ggtaaagtcg atctctgggg gcaacgcttt attgaagtat ggtcggggat
     6961 gccgacgctg tttttgatta ttttactttc cagcgtcgta cagcctaact tctggtggct
     7021 gctggcaatt actgtcttgt ttggctggat gagtctggtc ggcgtggtgc gggcggagtt
     7081 tttacgtact cgtaatttcg actacattcg tgcggcacag gcgcttggcg tcagcgatcg
     7141 cagtatcatc ctgcgtcata tgttgcctaa tgccatggtc gcgaccctca cctttttacc
     7201 gtttatttta tgtagttcga taaccaccct gacctcgctc gatttcctcg gcttcggtct
     7261 accgctcggt tcaccgtcac tcggtgaact gctgttacaa gggaaaaata accttcaggc
     7321 cccgtggctt gggatcaccg ccttcttgtc ggtggcgata ttattgtctt tgctgatctt
     7381 tattggtgaa gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa
     7441 ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag
     7501 tagtcaatga tgtttcacta cagattgagg ctggcgaaac gctggcgctg gtgggtgagt
     7561 caggttcagg caaaagcgtt accgcgctgt caattttacg cctgctccct tccccgccgg
     7621 ttgaatatct ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgatc
     7681 aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat ttttcaggaa ccgatggtgt
     7741 cgttaaatcc attgcatacc ctggaaaaac agctttatga agtgctttca ctccaccgcg
     7801 ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc
     7861 gccaggcggc aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc
     7921 gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccga
     7981 ccaccgcact ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag
     8041 gcgagctgaa tatgggcatg ctgtttatta ctcataacct cagcattgtc agaaaactgg
     8101 cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga gcaaaataac gccgctacgc
     8161 tatttgcctc acccactcat ccttacacac aaaagctact caacagtgaa ccgtcaggcg
     8221 atccggtacc gttgccagaa cctgcctcaa cgttgctgga tgttgaacag cttcaggttg
     8281 ccttccccat tcgcaaaggg attttgaagc gcattgtgga tcataatgtg gtggtgaaaa
     8341 acatcagttt tacgctacgg gcgggtgaaa cactgggttt agtgggcgag tctggttccg
     8401 ggaaaagcac gacgggactg gcgctgctgc gactgattac ctctcagggc agcatcgtct
     8461 ttgacggtca gccactgcaa gatttaaatc gccgccagct gttacctact cgtcatcgta
     8521 ttcaggtggt atttcaggat ccaaactcct cgctcaaccc acgactcaac gttttgcaga
     8581 ttattgagga aggcttacgg gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac
     8641 aagtgatagc cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg
     8701 cggagttctc tggtggtcag cgacaacgta ttgcgattgc cagggcatta attcttaagc
     8761 cctcgctgat catacttgat gaaccgacat catcactcga caaaacggta caggcgcaaa
     8821 tattgacgct tttgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc
     8881 acgatttgca cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag
     8941 tagtggaaca aggaccgtgc gcgcgcgtgt ttgccacacc gcagcaggag tatacgcgtc
     9001 agttactggc gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt
     9061 gccacaccaa aatttttcaa tcggcatgtt gcagcaaact cgtcctggcg atttacaaac
     9121 aggcacggct caccttcaca ctccaacacc gaacatggaa cttcgatgtc gcttaacgcc
     9181 tggcttagtt tatacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc
     9241 gccaccaggc tgttgtcacc gcacggtcca ttttgaggaa tcggttcaac cttcgaacct
     9301 gcgaaaccag cagaccagcg atagttctgc ggcagtcgat ggacgattga gagttgtaat
     9361 gaagtcactt attttccccg gaagtacatt cacttcacaa tttgtttaca tcaattttaa
     9421 cacatcatca acaatccgtc tttatacaga tcgtaaagat tgatgcctcg cctgcgtggc
     9481 ctctatggct ctgatttaag taatatctgc gggcgtggtc acgtttgcgg ggctatatgt
     9541 acccgtttct gcgatctaac tcaacctttt taactacaat gatgtgactt tttacataaa
     9601 ttgattttac ataaaataaa catatatcgg ggaaatgata gatttgtggt tgacatgcat
     9661 caacaaagga agccttttac cttcctcgtt gtgcaataga tcaccgtttt ttcggccgac
     9721 tggcgtacag acagaagaga atggagctgg ttgcgcagaa tgcaattgac caaatcatcg
     9781 gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc aacaattgcc cctatgccaa
     9841 aacggaaggt tcctgccagc gaagatgccg ttcccgccat atggggaaac tcatcaagaa
     9901 tgaccgccat cgcattggat gacaccatcg acacgcagcc cacaaacgcc gcaacgccaa
     9961 ccaccagcga ccagaatccc agccccagcg gcgcactgat gaccatccac gcagccataa
    10021 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cggacgaagc
    10081 ggctgttaaa gatggtcatc acgaacagaa aaacaatgtt tagcgcaaag taataaccaa
    10141 agttttccgg cgcgacgtgg ttaatttcaa tataaacaaa cggtccggcg cttaagaatg
    10201 agaacatccc ggcaaagctg aaaccactgg caagcatgta gctcaggaca cgtttatggc
    10261 ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg aaatggctga cgacgctccg
    10321 gtggtaaggt ttctttaatc aggaagaaaa tcattgccga agccagaatc gccgctaatg
    10381 ccaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    10441 gtgccatcag cagtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    10501 cttctttcgg gtaaatatca cgcatcaaag cgttgataac cacgctggcc gcagccgcag
    10561 ccgcagccag cccgtggaag aaacgcatca caatcagctg atcgatggtt tgtgccaacg
    10621 cacacgccac cgcggcggcg gcaaacacca gcgtcccgcc aagaaccacc ggcttacgcc
    10681 cgaagctgtc tgccatcggc ccgtagatta actgccccaa cgcaaagccc agaatataag
    10741 tactgagggt catctgcgta ctgcccgccg atacgccaaa ctgcgctgaa attaccggta
    10801 gcgcgggcag atacatatca atcgacagcg gcatcaacat ggccagcagg ccaaggataa
    10861 aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac gggctcctga aagtcattga
    10921 aaagttagac gacgctggca atttcttctt cagttaacgg acgatattca ccgggggcta
    10981 aatcagcatc cagcgtaata ccgccaatac gttcacgatg cagctcaacc acgtggttac
    11041 ccacggcggc gaacatgcgt ttcacctgat gataacgccc ttcgctgatg gtcagacgaa
    11101 cctgcgttgg ggtaatcact tcaagcaccg ccggcttagt gagatctttt tcgttatgca
    11161 gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc tacaggtgat tccagtgtca
    11221 ccagataggt cttctcgcaa tgatggcgcg gagaagtaat gcggtgcgac cactgaccat
    11281 catcagtcat cagcaccaga ccggtggtat cgatatccag acgtcctgcg gcgtgcagtt
    11341 tccacgctac cggttcatcc agaaaataga gcaccgtcgg gtgatccggg tcgtccgtgg
    11401 agcaaacata gccctgaggc ttattgagca tgaagtaacg tggaccgtgt tgctgcgcca
    11461 gcgggttgcc atcgtaagcg acatcatgtt cagggagcag tttgaacgct gcattacgaa
    11521 cgatttcgcc atcgacggtg acacgattgc cgcggatttc acgcccggca atagcacggc
    11581 taacgccgag ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt ttgcctgtaa
    11641 aaaacggagg tcgggtaatg acccgaaaat ctgaactgtt gtctgcccag tatagcggtc
    11701 taataacttc cctcaaggga aaaagaaccg tggcatacta ttagcagaat aatctaccta
    11761 cgcgaaacca tgatttttac actccgccca tatcagcaag aagccgtgga tgccacgctc
    11821 aaccattttc gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg cgcaggtaaa
    11881 agcctggtga tagcggaact ggcacggctg gcgcgtggtc gcgtactggt gctggcacac
    11941 gttaaagaac tggtggcgca aaaccatgca aagtatcagg cgctggggct ggaagccgat
    12001 atttttgccg ccgggctaaa gcgcaaagag agccacggta aagtggtatt tggcagcgtt
    12061 cagtcggtcg cccgtaatct tgatgccttt cagggtgaat tttcgctgtt gattgtcgat
    12121 gaatgccacc gtattggtga cgatgaagag agccagtatc agcaaatcct cactcacctg
    12181 actaaagtga atccccactt acgcctgctg gggctgactg ccacgccttt tcgattgggc
    12241 aaaggctgga tctaccagtt ccattatcac ggcatggtac gcggcgatga gaaagccctt
    12301 ttccgtgact gcatttatga gctgccgctg cgttatatga ttaaacacgg ctatctgacg
    12361 ccgccagaac gactggatat gccagtagtg caatacgatt tcagccgctt gcaggcacag
    12421 agtaacgggc tgttcagcga agctgatctc aaccgtgagc tgaaaaaaca gcaacgcatt
    12481 acgccacata tcatcagcca gatcatggag ttcgccgaaa agcgcaaagg ggtaatgatt
    12541 ttcgccgcca ccgttgaaca cgcaaaagag attgtgggat tactgcctgc cgaagatgct
    12601 gcactgatta ctggcgacaa ccccggcgct gagcgcgatg tgttaattga agattttaaa
    12661 gcccagcgtt ttcgctatct ggtcaacgtc gcagtactga ccaccggatt tgacgccccg
    12721 cacgtcgatc ttatcgccat tctgcgccct accgaatcag tgagtcttta ccaacaaatt
    12781 gtcgggcgcg gtctgcgtct cgctccgggc aagactgatt gtttaattct tgattatgcg
    12841 ggtaatcctc acgatctcta cgcgccggaa gttggtacac cgaaaggcaa aagtgacaac
    12901 gttccggtac aggttttctg ccctgcctgc ggttttgcca acaccttttg ggggaaaacg
    12961 accgccgacg ggacattgat tgaacacttt ggtcgccgct gtcagggatg gtttgaagat
    13021 gacgacggtc atcgcgaaca gtgtgacttc cgtttccgtt ttaaaaattg cccgcaatgt
    13081 aacgcagaaa atgatattgc cgctcgccgc tgccgcgaat gtgacacggt actggttgat
    13141 ccggatgata tgttaaaagc ggcgctacga ctgaaagatg cgctggtatt acgctgtagc
    13201 ggcatgtctt tgcagcatgg gcacgacgag aaaggcgaat ggttgaaaat cacctattac
    13261 gatgaagacg gcgcggatgt gagtgagcgt ttccgtctgc aaacgcccgc ccagcgtacc
    13321 gccttcgagc agctttttat ccgcccgcat acgcgcacac cgggcatccc tctgcgctgg
    13381 atcaccgccg ccgatatcct cgcccagcaa gccttactgc gacatccgga ttttgtcgtc
    13441 gcccgcatga aaggtcagta ctggcaagtg cgtgaaaaag tgttcgatta cgaaggtcgt
    13501 tttcgtcggg cgcacgaatt acgcggttaa tggtagtttt gattgatgta taaggcgatt
    13561 gagtatagaa tctcgcccgc ttttgcatac gcaaagcaga tcacttacct gttgctgggt
    13621 cgcctgtagc aggattaatt taagagagaa agaaatgttt actatcaacg cagaagtacg
    13681 taaagagcag ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt tcccggcaat
    13741 catctacggt ggcaaagaag caccgctggc tatcgagctg gatcacgaca aagtcatgaa
    13801 catgcaagct aaagctgaat tctacagcga agttctaacc atcgttgttg acggtaaaga
    13861 aatcaaagtt aaagctcagg acgtacagcg tcacccgtac aaaccgaagc tgcagcacat
    13921 cgacttcgtt cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc ggcggggttt
    13981 tttgtatctg cagattatgc ctgattacgg tattgctatt ttttgcaggc cagataaggc
    14041 gttcacgccg catctggcat tcagaacaca aaaccgatta attgccgcca gacgtccggc
    14101 gctgcaattg gtcgcgcaaa ttcggcggcg tgcctttaat ggtcaaggta tcggttgctg
    14161 gatcccagaa gatgcgttca cctaacagca tcgcatcaaa gttaatagtc aagccaccgc
    14221 cgctaccggc aaacttcgtc aactgacgca gtgtgctacg gtctgccggg aagctctctt
    14281 ccagttcgta gcctttttca gccgcaaact ccgtgaagct cacttcgctt acgcctgcca
    14341 gctctttcga cagcgattcc agttcgatct cttcgcctgc ctgcagctgc tcattacagt
    14401 agctataaac ctgctggcgc acgttctgtc gctctgcttt atccagttgt gcttcggcag
    14461 tgaaatcatc aaccgcttgc aacagcccac ggttttgcgc tttcgcgttc aatccttcgc
    14521 tggccccgag gaaatccata aagaaatccg ccactttgcg ccctactcgc cctttcagga
    14581 aagtgagata gcgggtggac tctggattgg tttcccattc ggttaaatcg atacgcgcaa
    14641 caatatccgc atgattgata tcaagataat gggttgggtt gatgtcgaga ttttcgttaa
    14701 cgcgcatact gctcaggttg ctcagaactg ccaccagcaa atactccacc gccagataac
    14761 ggtagtggca aaacagcacg aatccaccgt cagcaaaagg atatttcgct aattcgtcac
    14821 gcaggcgacc ggttgccgcg cggctaaatg ccagaaagtc ctcttcgccc tgacgctgta
    14881 agcgcagcgt ctgcgccagt tcactctctt cgctaaacaa gccgtaagct ttatttttgg
    14941 cgctatagac ccgatgcagt tctgccacca tctcaacgac ggtttctgtc ggttccagca
    15001 atgaatcgcg caacaccagc tcaaggttct gctcatcacg cttgataagc tggtgcagag
    15061 caatctggtt gatatccaga ctcatgataa actctccttt aagaccgggc ggtattcaac
    15121 caccgccagt taagcgacgc aataaaagat ggcggtcatt ttacagaagg cagcgtaacg
    15181 agaatgattt aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa tatgttgccc
    15241 tttcatcaac taactgattt cgatttatgc cacaaatttc ccgctacagt aatgaacagg
    15301 ttgaacaatt gctcgcagag ctgctcaacg tactggaaaa acataaggct ccgaccgacc
    15361 tttccctgat ggtgttaggc aacatggtca ctaaccttat caacaccagc attgccccgg
    15421 cccaacgcca ggcaattgcc aactcttttg ccagcgcctt acagtcctct atcaacgaag
    15481 acaaagcgca ctaagggaaa cagataacag gttatggtaa ctcatcgtca gcgctaccgt
    15541 gaaaaagtct cccagatggt cagttggggg cactggtttg cactgttcaa tattctgctt
    15601 tcgctcgtca ttggcagccg ttacctgttt atcgccgact ggccgacaac gcttgctggt
    15661 cgcatttatt cctacgtaag cattatcggc catttcagct tcctagtgtt cgccacctac
    15721 ttgctgatcc tcttcccgct gacctttatc gtcggctccc agaggctgat gaggtttttg
    15781 tccgtcattc tggcgacggc gggaatgacg ctattactga tcgatagcga agtctttact
    15841 cgtttccatc tccatcttaa tcccatcgtc tggcagttgg ttatcaaccc agacgaaaat
    15901 gagatggcgc gcgactggca gctgatgttc atcagcgtgc cggttatttt attgcttgaa
    15961 ctggtgtttg cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg tcgacgcttc
    16021 gcgcgcccgc tggccgcatt cttatttatc gcctttatcg cctcgcatgt ggtgtatatc
    16081 tgggccgatg ccaacttcta tcgcccgatc accatgcagc gcgctaacct gccgctttcg
    16141 tacccgatga cggcgcgacg tttccttgag aagcatggcc tgcttgatgc gcaggagtat
    16201 caacgccgtc ttattgagca aggtaatcca gacgccgttt ccgtacagta tccgttaagc
    16261 gaactgcgct atcgcgatat gggcaccggg cagaatgtgc tgttgattac tgtcgatggc
    16321 ctgaactact cacgcttcga gaagcagatg cctgcgctgg caggttttgc tgagcaaaat
    16381 atttcgttca cgcgccatat gagctccggc aacactacag acaacggcat ctttggcctg
    16441 ttctatggca tctcgccgag ctatatggac ggcattctgt cgacccgtac gcctgcggca
    16501 ttaattactg cgcttaatca gcaaggctat cagctggggt tattctcatc agatggcttt
    16561 accagcccgc tgtatcgcca ggcattgttg tcagatttct cgatgccgag cgtacgcacc
    16621 caatctgacg atcagaccgc cacgcagtgg atcaactggc tgggccgcta cgcacaagaa
    16681 gataaccgct ggttctcgtg ggtttctttc aatggcacta acattgacga cagcaatcag
    16741 caggcatttg cacggaaata tagccgggcg gcaggcaatg tcgatgacca gatcaaacgc
    16801 gtgctcaatg cactgcgtga ttctggcaaa ctggacaata cggtggtgat tatcactgcc
    16861 ggtcggggta ttccactgag cgaagaggaa gaaacctttg actggtccca cggtcatctg
    16921 caggtgccat tagtgattca ctggccaggc acgccggcgc agcgtattaa tgctctgact
    16981 gatcataccg atctgatgac gacgctgatg caacgcctgc tgcatgtcag cacacctgcc
    17041 agcgaatatt cgcaaggtca ggatttgttc aaccctcaac gccgtcatta ctgggttact
    17101 gctgcggata acgatacgct ggcaattacc accccgaaaa agacgctggt gctgaacaat
    17161 aacggtaaat accgcactta caacttacgt ggtgaaagag tgaaagatga aaaaccacag
    17221 ttaagtttgt tattgcaagt gctgacagac gagaagcgtt ttatcgctaa ctgattaatt
    17281 ataaatcagt tagcgaaata tcttacttgc aatcggtgtg gaaaacggta gtattagcag
    17341 ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc gggggtcgga
    17401 ggttcaaatc ctctcgtgcc gaccaaaaat cccaagaaaa aaccaaccct tacggttggt
    17461 ttttttatat ctgcaattaa ttcgataaac agaccgtgac acatcacagc ctgtttattt
    17521 tctgttatca gaacgtccag accacacccg cctgagtatt ccacggcgat tctacccctg
    17581 cgccattgcc atagctgact gacaaatgac cgcttaacgt cggggtaaac gatgacctta
    17641 tcccagcctg ataaacgcca ctggttccag ccacatcatt attaaatttg ccatcgtcat
    17701 taactttcac ctggttagaa tcggcgtatt cctgacgcac ggcggctttc agccagggtt
    17761 ccagcgtcgt accgttttgc aggtccatgt gatagcttac cgccgttccc gcttcagcgc
    17821 gtaatatccg ggtatttccc acatccgcgc gcatgccgtt tgataacgtg tagtcctgac
    17881 catctgtggt aaagccggta aaggccagat agggtctaac actccacaat ccgtcaaccc
    17941 aacggaaccc gctctcaaca tgagcacccg cgccgttact attgtaatcg ccaaacgctg
    18001 ttgccccatt actcatcttg ccatggatgg tgttggcaaa acggtcaact ttcaccaccc
    18061 catcaacata ggcaccgttc tgatgctccc aaccggcata agcccccagg gtatagctat
    18121 cgatattacc ttttccgccg cgatcaaaac caatatcaga atgagagtaa ccaaagaaca
    18181 agccgcgaat tgtactgctt tcttcacggg agaaacggct atcgataccg agcgtcaggc
    18241 ccgtcaatgt ttgctcaaaa ccagctcccg catcagtggt cacgttgttg cgggtgttaa
    18301 ttgccgaact ccacatcacc gtatcgtaac taacgccttt tacgctacca agacgctcac
    18361 gcacggtgtc cagttctgca tcaaatacca gcggttgtgc ggctgccatg ttcagcacat
    18421 cagtggttga aggggtaatt tgcgcgcgat tctctgcgag gctccagcta tggttgccat
    18481 tatccagcaa ggtatattca tacgtaccga tatcaacaac gcctccggca ttgcccaacg
    18541 taaatgcagc atcaccgccg cccgttgtta ccagtgtaag gctatctcct gctgccgggc
    18601 tggcaccggt gtccgtcacg aatattttga aatcacctgt tgcctgaccg gtgacgttga
    18661 gctgatcgct ctgatgattg gccatatcgg tacgcatata aaaattgccg tttcccgaca
    18721 gggtattggt ggtcaacgta atgaaatttt ccgcacttgc cgtcacagat gaacgagtca
    18781 ctggctcagc catcataatc acatttgcgc cggacatcgc aaggttggct aagttgaggc
    18841 gagtgttttg gttcgtcgta gtgatatcgg caatgatggt ccatgcgcta ttttcgagcg
    18901 aaacgtccgc tttgctggta tccacggcac catgcgttct aaaagaagcg ccctcagaaa
    18961 ccactacctt acccaccaac atagcgggtg cataatttat ttgcggcttc atgacctcaa
    19021 gaatgccatc attccctctg actgacacgt taaccattgt gccagcccag acattagcgc
    19081 ggccattgtt gattaccata ttttcagaga tggcttttga ggaatacgtg atacttccat
    19141 tctggtctga tcgaccgaga tcatataccg catttgcctg cactttcgta ccagtatcct
    19201 ttcccagcga ttgcatagtg gcatgctcat caaggatagt gtcaatggcc tgcgtgtcct
    19261 ccataacaat gagcccggaa ccatcatcca gttcataatt ttttgacaca ccatccttta
    19321 tactaaattg gcctttactg tttgtaccac tcacttccag cgcattcgtt gagacaatta
    19381 atttcccgcc tgctttttta tcaacgccag ttgcagtccc gccatccata acctccagta
    19441 agccgccact atccacagta gtattataag cgaagtcatt ttcttcaact cgcaaacttc
    19501 cgccgttttc caacaacata ttattagcaa taccattctt gatatcgaac tgaccgagac
    19561 ggtttgttcc gaatacctcc atggcccgcg tggttgtttt aatagcaccg cctgttttct
    19621 gatctaccgc aagcgcaaat cccccctctt taacctccag tagtcctccc ttttcgatag
    19681 cggcaaaaac ggccccccca tctttttcaa cgattaagcg cccgccttta atcgtggtat
    19741 cattcgctgg gccataaacc tgtagcacac cattattgag ggtagtctgg tttgccgcgc
    19801 cacccatttg aacaatttga taaccatcat ttaatactga gtattctgcg gtaccattca
    19861 tttcaatgta ttgataccct ccgttaattt cagtattact acttacacca aattctttta
    19921 tatgctgttc tccgccagga ttgattttcg tattattggc ggtcccataa accagctgga
    19981 tatctttttc caggataaca ccatcaactg tctcaccata ctcaactgtt gatgccaatg
    20041 ctatgggtga atataacgct gtagccacca gaacagatat tgcggaacga caaagagaaa
    20101 cagaaccaga ttgatgcatt gagctttcat cctatgaaat taattgcagt taaaagcatt
    20161 gggtacagaa aatacccata gctccatacc cggagtcagt ttttaaaaac tgtttaaaga
    20221 aatgcacaag tattgtgatt gattttttag ttgtttttct tgatgagaag ctgatgcaaa
    20281 attccgtctt tataataaaa atgatgccaa agcgaacgac aaggttgtag ttttcactac
    20341 atgtccatac ataaaatggg gtaacattca cgcgcctggt agcgttacca acgctacgct
    20401 caaacataat gattctaata aaacctcagg agactactat gcctgaagca acgccttttc
    20461 aggtgatgat tgtggatgat catccactta tgcgacgcgg tgttcgtcag ttactggagc
    20521 ttgatcctgg ctttgaagtg gtcgccgaag cgggcgaagg cgcgagcgcg atcgatctgg
    20581 cgaatagact ggatatcgac gtgatcttgc tggatctcaa tatgaaaggt atgagtggcc
    20641 tggataccct caatgccttg cgcagggatg gcgttaccgc gcaaattatt atcctgaccg
    20701 tatccgatgc ctccagcgat gtctttgcgc tgatagacgc aggcgcagac ggttatctgt
    20761 tgaaagacag cgacccggaa gtattgctgg aagcgattcg taccggagcg aaaggcagca
    20821 aagtctttag cgaacgcgtc aatcagtact tacgtgaacg tgaaatgttt ggcgcggaag
    20881 aagatccctt cagcgtgctg acggagcgcg agctggatgt tctgcacgag ctggcacagg
    20941 ggctgtcaaa taaacagatt gcatcggtgt tgaatatttc cgagcagaca gtaaaagtac
    21001 atattcgcaa tctgctgcgt aaactcaatg tccgctcacg cgtggcggcc accattctgt
    21061 tcctgcaaca acgcggggca caataaaaat agcccgatgg atttatcatc gggctgagat
    21121 ttatgacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt ggatcgatca
    21181 atttattgaa tttacacaat tttgtaggcc ggataaggcg ttcacgccgc atccggcata
    21241 aactaagcgc actttgtcat cagtctagcc cgatggcatc accatcgggc ctctttttat
    21301 ttactctcct gcggcgacaa atgttgcatc gcctgcgcga tactacgttc aatcaccgca
    21361 cggcgagtat cgttggcagg taagagtttc aacatcatct cccacgcggc aacggcttcg
    21421 ccaaatcgct gctgctcaaa ggcattaaac gcatacatgc ttagcacacg gatattgcta
    21481 tggtccgttc tcaccagctg acgtagcagt tcaccgccga ggcggttgtc gttgggatca
    21541 gatgaacgag tcaacgcttc agcgtatccc agtgcggcat cgctgttctt cggatcgagg
    21601 cgatacgcag tagcgtatgc atcggtggcg atactggcgt tacccagcgc catgccaacg
    21661 cggcccaaca taatccagcc ttctatatct cccggatttt tttgcagttg agtacgcatc
    21721 cccagcgcaa ggcgcgacat ctcttcttcg ttgagcggat cggctttcgg gtccagcgcc
    21781 ctgtccagca acgccggagc ctgtgccgtg gcctgctgcc agattttcac ctgctgataa
    21841 ttgccagtct ggtagtagct gacgccagcc acaattaacg ccaccacaat acccggcaga
    21901 taaacaacat atccggcacg cttaccttcc ggcacgcttt gttcaggaaa cgcttccggc
    21961 accacgcgta cccgacgccg cgagcgggcg tatatgaccc aaccgccaat gccaatagcc
    22021 actactggca gcacccacag cagcacggtc agcggcgtta acggcggatc gtaagtgacg
    22081 aagttgccgt aacgcgccac catataatcg acaatctctt ttttactttt accttcctgc
    22141 atcagttcat acactttctg acgcaggtcg gtggcaatca tcgagttgga atcggcaatg
    22201 ctgttgttct ggcatttcgg gcagcgcagt tcttcagtga gctgacggaa ctgttgttcc
    22261 tgtgcttcat ctttaaactg caacacgtcg atggtcgcca gcgctgagcc ggagatcatc
    22321 agcatcagca cgcccaataa aaacctcatt gtgcggcctc cttactgtat ttctcccaca
    22381 gcggcttgat ctcttcttcc cagacgcgag gattgagatc gcccgcatgg cgatagcgaa
    22441 tgataccgtt gccgtcaata aggaaggttt ctggcgcgcc atagacaccg agatccagcc
    22501 ctaacatgcc gtcgccatca aacaggctta gcgcgtaagg attgcccagc tctttcagcc
    22561 agctgattgc cttctggcga tcgtctttat agttcatgcc gaccacgcga atgccctgcg
    22621 ctgaaagctg attcagatat tgatgttccg cacggcaggt cggacaccaa gtcgcccaga
    22681 cgttaagcag tactggtttg ccctgagtca gcacatccgc ctgataaaac tgccccggat
    22741 tgtccagtga ttcgagacga aacttcggca caggcttgcc aatgagcgcc gattccagat
    22801 tggtcggatc atccccttcg gcattacgcg ccagctgcca cagcagcgcc gcggcaatcg
    22861 ccaggaagat aatcaacgga attaacaata ctttgcgctt catacggcct ccggcgcagt
    22921 tttttgcgga ctcacgcgct tacgatagcg aggatcaaac agacacagca gtccgcccaa
    22981 cgccatcatc agcccgcccg cccaaatcca gcgaacaaat ggtttgtagt aaagacgcac
    23041 ggcccacgcg ccgttttcca gctcttcacc gagcgcggcg tacaggtcac gcgtgatgcc
    23101 gccgtcaatt gccgcttcgg tcatcatcga cccggcagtg ttgtaataac gtttttccgc
    23161 atacagcacc gtttccggtt taccctctcg cgttacgcca atagtcgcca caccgccgcg
    23221 ccagttcggg ccagtcacct ctttgacatc acggaaggtg aagcgatatt catgaatatc
    23281 gacgctatcg ccggacttca tgcgcacatc acgctcaacg ctataattct ggctaaaggc
    23341 aatgccaaca attgtcactg ccagccccag gtgagccgcc accatccccc aataactgaa
    23401 ggtggttttc gtgccgcgtg aaatacgcag cgcagcttcc gcagttgcca gcaccgcaat
    23461 ccagcaggcc attgccaggc cgagcaccgt catcgccaca actttgcttt cgaacagcca
    23521 cggcaacagc agagacagca ccagcgtaga gatgaaggcg ataatcaata aattgcggat
    23581 tttacgtggg cgatcgcgcc cccagcgcac cagaggaccg acaccaagca gtagcgcaaa
    23641 cggcaccatc agccaggtaa acatggtgtt gaagaacggt tcgccaatcg aaatactgcc
    23701 cagtccaagt tgcttgtgca ccagcggcag cagcgtcccc agcaacacca ccagcatcgc
    23761 ggcgaccagc aaaacgttgt tcgctaacag caaagattcc cgcgaccaca gcgcattgtt
    23821 tacgcgtgag cgaactttgt gtccacgcgc ggcaaacagc agcagcgaac cgccaatcac
    23881 cagcaccata aaggcgagga taaacatacc gcgcgccgga tcagacgcaa acgcgtgtac
    23941 cgataccagc acgccggaac gcacgaggaa agtccccaac agacacaacg agaaggcact
    24001 gattgccagc agtaatgtcc acgctttgaa gctggcgcgt tgttcagtga ccgccagtga
    24061 gtgcatcagt gcagtcccca ccagccacgg cataaacgag gcgttttcta ccggatccca
    24121 gaaccaccag ccaccccagc cgagttcgta ataggcccat gcggaaccga gcacaatgcc
    24181 gagcgttagg aaaatccacg ccgccagcgt ccacgggcga gtaaaacgcg cataagtgct
    24241 gtccagacgc ccgctcagca aagaagcaat ggcaaaagca aacgccaccg agaaacccac
    24301 gtaccccata taaagcagag gcggatggaa gatcagcccc ggatcctgta acagcgggtt
    24361 aagatcgcgc ccttcaatcg ggaagttcgg cagcgtgcgt gagaacgggt tagaggtaaa
    24421 gagaatgaac agcaaaaagc cgacgctgac catccccatt atcgccagta cacgggccac
    24481 aatatccagc ggaatacgct gactaaaaat cgccaccgca aaggtccagc cgctcatcag
    24541 cagcacccac agcagaagcg aaccttcatg cgcgccccaa gtagccgcca cgcgatacca
    24601 caccggaagc tgggtattgg agttgctggc aacataggtg acggtaaagt cgttgaccac
    24661 gaaagcattg accagcacca gaaatgcgcc agccacagac ataaacagca gccaggcaaa
    24721 caagcgggaa gacgccatca tgcgcgcatc tccgcgcgcc acgccccata gcggatacac
    24781 ggacagcagc agcgcaatcc ctaacgccaa gcacagcagc ccgtttccaa tttccggcat
    24841 catgatgctg ggtccttata aacactcgcc gggcgacggt ggttagcttc catcgctttc
    24901 tcaacttctg gcggcgtgta gttttcgtcg tgtttcgcca gcacttcttt cgcgaggata
    24961 tgattgcctt tttccagctc gccctgcacc acaacgccct gcccttcacg gaacagatcc
    25021 ggcaaaatgc cttcgtaaga gacatccact gagccttcag catcgtaaat ggtgaaggtc
    25081 actttcagcg aattgggatc gcgctgcacg ctacccggca tcaccatccc gccaacgcgc
    25141 agacgctgac cgacttccgg catctgctga gtttcacgct tgccgtagag aatttccccc
    25201 ggcgtataaa agagatcgat attcgagcgc agcgcatata gcaccagacc gatagtcagc
    25261 gctagccctg ccaacacggc acaggcaatc cacaagcggt ttttacggcg aatattcatg
    25321 cagcctcctg ctgttgcgca gcacgtaaac gcgcctcacg cgcccgctgt tgcgccacac
    25381 cacgcagaat tgcgcgatgt tgcatcaccg agtgcacgac caaaaccacc agcggaataa
    25441 cggtcatcac caccgccagc cagacaaaaa aggcgtaacc gcccattgcg aaaaattcat
    25501 tccaggaagc aaatgcaggg gtcatttacg gcctcttttc agtatcagtt cactcaccca
    25561 cggacggcgt ttttccatca gcaaaatcaa attacgcatc cgcatcagcg tcagcgtggc
    25621 agacaggagc aggaagccaa aaatcgacca gcgcagcggc gaacgcatcg ccggatcgat
    25681 actttgctgc atccgcgttg atccctgatg cagggtgttc caccactcca cggagtaatg
    25741 aataatcggc agattcacca cgccaatcag caccatgata cctgccgcac ggcccgccag
    25801 acggcggtcg tcgaaggcgt gccacagggc aatcacaccc acatacaaaa acagcagcac
    25861 cagttcagaa gtcagacgtg catcccatac ccaccaggtg ccccacatcg gttttcccca
    25921 tgcagagccg gtaaccaggg caataaaggt aaacacggca ccaatggggg ccatcgccgc
    25981 caccgccagg ttggccattt tcatctgcca gacaaggcca ataaacgccg ccaccgccat
    26041 tgatgcataa atgcccatcg accagatcgc cgcaggcaca tgcaggtaga taatgcggta
    26101 gctatttccc tgctgataat cagccggagc aaagccgaat ccccagatcc agccgacggt
    26161 aagcacgacc acactggcaa ttgccagcca cggtataaac cagccacaga tttgatacag
    26221 ccgtggtggg atcgccagtt gatgcagtgt tttccacata gtttcgatac cagactcgaa
    26281 caaaaatcag taatccagcg ttattgaatg ctgattcgta acgctgccgc cgtcgcaaaa
    26341 ggacttaatg tcgcggtgcc tgccagcaac gcgcctaaaa ttgccagata cccatcaacg
    26401 ggcaaatgca tagaagccgc gtccatcgcg gcggtggcaa agatgagtaa tgggatagtc
    26461 agcggtaaca ccagtatgct gagcagcaca ccaccgcgct taagtcccac tgtcagcgcc
    26521 acgcccggtg caccgagaaa gccaagcgta ggcgttccca gcagcagcgt cagcgccatc
    26581 acttgccagc cataaacatc cattcccagt agcattgcta ccagtggcga aaggatgagt
    26641 aacggcagac cggttaccat ccagtgcgcc atcaccttcg ccagcacaac ggcgggcaag
    26701 ggtaacggca acaacatcaa ttgttcaaga ctgccgtcct gcaaatcgtc acggaacagt
    26761 cgttccagcg ccagcaagga tgaaagcagc gcagcaaccc agataatgcc cggtgcaata
    26821 cgcgccagca gttgcggctc cggaccgata ctgagcggaa aaagggtaat tacaatcagg
    26881 aagaaccaca gcgggttggc gatttcggcg ctatggcgaa acgctacgcg cagttcaaga
    26941 cggaaaatac gccagaacat catgcggccc tcgtttgcgt cagtgaaatg cggcgaattt
    27001 tactttcagc aacgttgagc ggctggtggg tagtcagaat cacaatcccc ccctgctccg
    27061 tatgctgcgc catacgctgg gtcagacgat cgacgccgtt aacgtcaatc gcggtaaaag
    27121 gctcgtcgag gatccataat gtggcacggg tcaaccacag acgcgctaaa gcgacgcggc
    27181 gttgttgccc ggcagaaagt tgatttaccg gaatatcttc gaatccggca agcccggcct
    27241 gcgccaaggc ttccagacat tgtgcggcat cgccatcgcg atgataaaag tgcagatttt
    27301 ctaacgccgt cagccgggtt ttgatccccg gctgatggcc tatccataac agggtttgat
    27361 ggtagctgtc gcgtacctga tgcaagggct gcccttgcca gagaacctcg ccagcgtcag
    27421 ggcgagacaa ccccgtcagc aaacggagaa gcgttgtctt ccccgcgccg ttgctaccgg
    27481 tgatttgtac ccactctcct gcgttcagcg taaatgacaa gccactaaat aaggttcgtt
    27541 catcccgctc acaaagtaac tctctggctt caagcatacc cacgcaataa ccctgttaaa
    27601 aacctggctc gacttcacgc atatcgggca gcttgtgcgc gatcccttta tggcaatcaa
    27661 tacaggtttg cccatctttc accgcctggt catgcatctt cgcggcaacc gatttctggg
    27721 cggttgtatc catatactcg aagttgtgac agttacggca ctcctgcgag ttattgtcct
    27781 tcatgcgccg ccactcattc tgtgccatcg tcagacgatg agcttcaaat ttctgcggcg
    27841 tgtcaataac gccaaaaatt ttaccataca gctctttact tgctttgagc ttgcgtatca
    27901 tcttcggcac aaactcgtgc ggaacgtgac aatccggaca ggtcgcacgg acgccgctac
    27961 ggttgttgta gtgcacggaa tccatgtatt cctgatacac cgtgttgcgc atttcgtggc
    28021 agctaatgca gaactcttcg gtattggctt tttccatccc ggtgttaaag cccccccaga
    28081 agacgatgcc gccaacaaaa ccgatcaaca gcagcgtccc cagcgccaga tggctggggg
    28141 tacgccacca tttccacagg cgcttaatca gaccaggctt acggtcagaa tttcccataa
    28201 taacctctta tttcccgtaa ccttttgatg gggtaaaggt attccccacg attggcgcgg
    28261 tatcggcctg cggtacgtga cattgcagac agaaataacg acgcggagcc acttccgcgc
    28321 ccactttgcc gtcgctgtcc ataaagtgag taggactgat acgcggcgcg ccagtggtgc
    28381 gatagctttc gacaccgtgg cactgcaagc agcgattggt attggtcgtt acctgataac
    28441 cttcaacgct atgcgggatc attggcggct gattcacata gttcagcggc atccgatcct
    28501 gctcttttgg catccgaatg gccccttcct gcgtcccgga tacttccggc gactggctaa
    28561 aatcgacgcc attagccgcc caaaccgcgc cgcttaccac cagggccagc atcgccgtcc
    28621 attgacacag cgctttcttc aggtcatggc ttttcatgat ttcgctcccg aactccatcg
    28681 tgtagttatt gtaaatacat cctcagaaca gacatccacg cagcgaccgc acgtcatgca
    28741 atcgcggctg gtgacctgca ccgggctttg ttcatccagc accggggcac gtagcacatg
    28801 cggttccggg caaacatgaa aacaatccat acagcggtta catttctgcc gatcgctggc
    28861 cgcaacggta atcacacctt tgctacccag cacgccatac agcgcgccaa ccggacaaat
    28921 gtgcccgcac cagccgtgtt caacgaccag tagatcaaat aaaaacagcg cgagaataag
    28981 cagcgcgccg ctgccgaagc ccattaccag gctacgcccc atcagagaaa cggggttgat
    29041 ccattcccaa atgagcgtgc cagttaaggc tgatcccacc agaatgacca ccaacagcac
    29101 gtagcgaatg tggcgaggga tcgtcgcaga ctgattgagg tcaaagcgcc tgcgtaacca
    29161 gttcgctaag tcggtaatcg ggttcagcgg gcagacccag ctgcaaaata atcgctttcc
    29221 cgccagagcg tagagcacgg tgataatcac cgcaccggtc aacgccaccg tggcaggcag
    29281 atgaccactg gcaagacttt gcagcgtcat cagcggatcg gtcagcggaa cggtgtcgaa
    29341 taacaggcta ctgctgtagt tgccgtgcaa gatccacaca ccaaaccatg gaccgctcaa
    29401 aaacatcccc agcacgaaga actgacaaag gcgacgtaac accagccaac ggtgactgcg
    29461 ccaccagcct tttttctcca gcgcctcgcg cccggcgtca cgtttacgat ttgccattgt
    29521 tcccctccag ccagccgaag cggtaatggt gacctaactc ccctttcgcc agtgacagcg
    29581 gtaacacctt gattgccggt tgttccagca cgcatacttt ttcgcactta ccgcaaccgg
    29641 tacaggcgtc gctgtgaacc gtcggcagaa agcgggcgtg cttaccggta cgcgtgttgc
    29701 gctccagctc caaggtgatg gcctcatcaa ttttcgggca ttcgcgataa caaacatcgc
    29761 agcgcagccc ctgaaagttg agacagtttt cctggtccac cagtaccgcc agccccatcc
    29821 gcgcgtcgtc gatcgattca atctcacgat ccagcgcacc gcttgggcac actttggcgc
    29881 acggaatgtc ctcacacatt tcgcaaggaa tatcccgtgc gacaaaatat ggcgtgcccg
    29941 ccgacagacc agaggccagc gtcgccagtt ttaaggtgtc gtaagggcaa gcctgaacac
    30001 actgaccgca acgcacacag gcactggcaa aggcgttctc gtttatggct ccgggcgggc
    30061 gcaaccgcac gccagatgcg cgtgcggttt gctgttgtaa ccccagcgcc acacccacgg
    30121 cagccagccc gcctgctgtg cgaacgacat cgcgcagaaa gcggcggcga ccattttgag
    30181 gtttcgctga ccgggacata atggcttaca ccttctccag tttgaccgcg cacttcttga
    30241 agtccgtctc tttcgagagc ggatcggtcg catccagcgt cagtttgtta accagctgtg
    30301 cggcgtcgaa gaacggcatg tacaccaggc cctgtggcgg acggttacga ccgcgcgttt
    30361 caacaatcga gatcacttcg ccacggcgag aaaccacttt cactttgtcg ccacggcgca
    30421 gatcgcgcgc tttcgcatcc agcgggtgaa taaacaagac cgcttccggg aaggcgcggt
    30481 gcagttccgg cacacggcga gtcatactgc cggtgtgcca gtgctccaga acgcgtccgg
    30541 tagagagcca caggtcgtac tcttcatccg gtgcttccgc cgccggttcg aacggcagtg
    30601 cgaagatcac cgctttgcca tccggtttac cgtagaactt atagccttcg cccgctttca
    30661 cgtacgggtc gttaccttcg ctgtaacgcc actgtgtttc tttaccattc accaccggcc
    30721 agcgcagacc gcgcgctttg tggtagtcat cgaacggtgc cagatcgtga ccgtgaccgc
    30781 gaccaaacca tgcgtactct tcgaacagcc ctttttgcag atagaagccc agctcgcggg
    30841 attcatcgtt cagctgatct tccgccagtt cggataccgg gaatttgctc acttcggggg
    30901 tggcatacag aacttcgtac agcgttttgc cacgcagttc cggtttcttc gccagcagct
    30961 cttccggcca tacttcttca gttttgaagc ggcgggagaa ctggactaac tgccagagat
    31021 ccgatttcgc ttcgcctggc gcctgtacct gctgacgcca gaactgagta cggcgttcgg
    31081 cgttaccgta agcgccctct ttctctaccc acattgcggt cggcaggatc aagtcggcgg
    31141 ccagcgcact gactgtcgga tacggatcgg agacgatgat gaagttgcgc ggatcgcgcc
    31201 agcccggcat acgctcttca ttgatgttcg gcccggcctg catgttgttg gtacacatgg
    31261 tccagtaaac attcagcttg ccgtctttca gcgcacggtc ttgtgccacc gcatgaagac
    31321 cgattttcgc cggaatggtg ccgctcggga tattccactt cttctcacag atatcacgat
    31381 gtttctcgtt agttaccacc atgtccgcag gcagacggtg agcaaaggtg cccacttcac
    31441 gcgcagtacc acacgcagaa ggttgcccgg tcagggagaa cggaccgcaa cccggctggg
    31501 aaattttgcc ggtcagcagg tgcaggttgt agaccaggtt gttagcccac acaccacggg
    31561 tatgctggtt gaagcccatc gtccagtagg agatgacttt cttgttcgga tcggcataca
    31621 gctgcgccag ttgttctaac tggtctttcg gcacgccggt catttcagcg gttttttcca
    31681 gcgtatactc agcaacgaag gctttgtaat cttcaaagct catcggttcg gaggcgtcag
    31741 aacccggatt cttcgctgct ttttccagcg gatgggtcgg acgtaaaccg tagccgatgt
    31801 ccgtcgcccc tttgcgcagg ttaacgtgct tgctgaagaa gtcctgattt atcgcattgt
    31861 tttgaatgat atagttggcg atgtagttca ggatcaccag gtcagattgc ggcgtaaaga
    31921 tgatgccgtt atccgccagc tcgaagctac gatgctggta ggtagaaagc accgccacgg
    31981 tgacgttctg gttagagaga cgactgttag tgatgcgtga ccagaggatc gggtgcatct
    32041 ccgccatgtt agcgccccac agcacaaacg cgtcagcctg ctcgatgtcg tcatagcagc
    32101 ccatcggctc atccatacca aaggtacgca taaagccaac tactgccgac gccatacagt
    32161 gacgtgcgtt cgggtcgatg ttgttcgaac ggaagcccgc tttgaacagc ttggacgcgg
    32221 cataaccttc ccagatagtc cactgaccag aaccgaacat accgatcgat tccggccctt
    32281 tttctttcag ggcggttttg aatttctctt ccatcacatc gaaggcctga tcccaggtga
    32341 ttggggtaaa ttcgccttct ttgtcatatt taccgttttt catacgcagc agcggctgcg
    32401 tcaaacggtc tttaccgtac atgatcttgg gcaggaaata gcccttaatg cagttcaggc
    32461 cacggttaac cggtgcgtcc gggtcgccct gacaggccac cacacgcccc tgctgcgttc
    32521 cgaccagaac gccgcaacca gtaccgcaga aacggcacgg cgctttatcc catttaatgg
    32581 cttcctgctg accaacaacg gcgcgggcaa cgcccggcac gctgagaccg gcagccgccg
    32641 cagcggccgc aacggcgtta gctttcataa agctacgacg actgagtttc atggtgtttc
    32701 ctcaccttgc tcttcctgct ggtgataaac cagcgacacc gccagcacgc cctctacgtt
    32761 gcgtactgac tcaatggttt ggatcagcgt ttcgctgtct tctgcttcca ccaccacaat
    32821 caactgaccg ctcggcgcgt cgctgacagc aacttcacag ccgggaaagg cgttcagttg
    32881 ggtgctgatg tctgaaattc gttcgctttt ggcctgcacg accaggctgc aaacttgcca
    32941 gttagtgtgc atggagatac tccgcagtta tggctgatac cggacagctg gctgcacatg
    33001 ctccacatcc gttgcaaagt tggctattaa gttgcgcctg gtagatcccg gagagcgtcg
    33061 ggcgaaagat aattgccatt ggctcacagc tatcctgaca gcggcggcat tcaactgact
    33121 gatacgccag gcacgcgtcc ccgatggtga actgcaaatc ccaggccctg gtgtggcgcg
    33181 gagaaaatag cgattcgggg caagcctgcg cgcaggcgta acagaagctg cactcgttat
    33241 ttttgaaatt aacgctcgga tagccacccg tgccgcgttg cagaatgttg ttttcacaag
    33301 cattaataca ggcgtcgcag cgggtgcaat gggtcagaaa atgagattca tcaccggacc
    33361 agggcggacg gataccgtta ctggctttgc gccagcaacc agtgagtatg ccccgacggg
    33421 atgcatcaat cttcacattg accttccatc attaacgcgc tctgaaaatt gagagcgacc
    33481 aaatagaccg cataattaat aagccatttt tatagccgct aagatattaa aggatgtgtc
    33541 aaagatgcat accccgatcg gggtaaaacc tgtagcagga tcaaaagagt ggcgggaagc
    33601 gtggcaaaaa cgggcttttg ctcacatttc aaatggttat aaacatattt atatagcgat
    33661 taattcacca gagatatttc tgctggtttg ctccctcatc agaatttaac actaaaagag
    33721 caggtaaaat tgtctgaatg ttctttaagt tattcataaa gcaaattaat aaatctgatg
    33781 aatatgttaa ccttcagcga catcatcggt gaaaacctat aaatgaagaa ggaaagcaaa
    33841 aaaatgaaga ccattctacc tgaagtattg tttgccgctt tcgctaccac ttccgcctgg
    33901 gcggcagaaa gcgtccagcc actggaaaaa atcgcgcctt atccacaagc tgaaacaggc
    33961 atgaagcgtc aggtgattca gttaaccccg caagaagatg aatctaccct gaaagtagaa
    34021 ctgttaatcg gtcagacgct ggaagtcgat tgcaatttgc atcgtctcgg cgggaagctg
    34081 gaaagcaaaa cgctggaagg ctggggctat gattattatg tctttgataa agttagctcg
    34141 ccggtttcca cgatgatggc ctgcccggat ggcaagaaag agaagaaatt tgtcaccgcg
    34201 tatctgggcg atgctggaat gctgcgttac aacagcaagc tgccgatcgt ggtgtatacg
    34261 ccagacaatg tagatgtgaa ataccgcgtc tggaaggcgg aagagaaaat tgacaacgcg
    34321 gaagttcgct aaactgttgt gaagtacggc accccgttgg ttggataagg cggttacgcc
    34381 gcatccaaca tctaacgccc gagccggttg cctgatgcga cgctggcgcg tcttatcagt
    34441 atacgccaca tcattacaac gcaatatccg ccacttcttt ttgaacgggt tgcggtttca
    34501 actgcggttt cggcgtacta tctgctgctt gcggtttgtc gtatttcagc cccagcactt
    34561 cgctggtgta ctgcaactcg cgttctgttg ccgctacatc accgttcagc ttgcgtccat
    34621 aagacggaac gatcgctttc aacgtagcct gccattgcgg gctggaaacg cgatcgccaa
    34681 atactttttc cagtagattc aacataatcg gcgcggcggt tgacgcccct ggcgatgccc
    34741 ccagcagcgc cgcaatggtc ccttgctggt cactgaccac ttcggtaccc agacgcagta
    34801 cgccaccttt ctcggcatca cgcttgataa tctgcacacg ctgccccgct tgccacaaac
    34861 gccagtcctc ttttttcgct tgcggatagt actctttcaa cgcttcaaaa cgatcctctt
    34921 cactcaacat cacctgactc accagatatt tcaccagatc gaaattatcc agcccgacgt
    34981 gcatcatcgg catcacgtta gaggtggtgg tggaactcat cagatcccac aatgaaccgt
    35041 ttttgaggaa tttggttgag aaggtggcaa atggcccaaa cagcactacg cgtttaccgt
    35101 ccagaacacg ggtatcgata tgcggaaccg acatcggtgg tgcgccaacg gatgctttac
    35161 cgtaaacttt cgccagatgg cgattaacca cgtctggatt ttccgaaaca aggaactgtc
    35221 cgcccaccgg gaaaccggcg tagtctttcg cttccggaat ccccgattcc tgtaacagct
    35281 tcagcgccgc accgcccgcg ccgataaaga caaatttggc gcggatgttc tgtgcagtgc
    35341 cgtttttcag atcggcaacg gtaacggtcc aggtgttatc gtcattacgc tttagggcgc
    35401 ggacttcgct gctgagttgc agcgagaagt tagatttctt ctgtaaggaa gcaattaact
    35461 ggcgggtgat ctcaccatag ttcacatcag tgccaatttc tgttgcggat cgcgcccttc
    35521 catcactaac ggtgcccact ctttgatctg cgcgtgatct tcagagtaac gcataccgcg
    35581 aaacagcgag ctttgttgca acgcggcgta acgggcgcgc aggaaattaa cgttatcctc
    35641 gccccagaca aagctcatat gcggaacggt attgataaat gaacgcggag tacgcagcac
    35701 gccgcgctcg acctggtgcg cccagaactg gcgggaaatc tgaaatgctt cgttaatggc
    35761 gactgctttt tcaatactga tgctgccatc ggcgttttgc ggggtgtagt tcagttccat
    35821 cagtgcagaa tgcccggttc cggcgttgtt ctagccgttc gaactctcct gcgcgccccc
    35881 ctcctggcgc tccaccatgg tcatcgacca ttcaggctcc agctcgcgtc aataggtccc
    35941 caacgtggcg ctcataatgc cgccgccaat caacagtaca tcagtttcct gctcctcgga
    36001 cgctttcgct tttgccgcca tcgaaacggc attaagcccc acggccatcg agaagagcat
    36061 ggcagtcact tttttcatct tgttaatgcc ttacttttag tcgctttatt gcaggtgaga
    36121 tttgcgcggc atcaacggta acacttaagt aacaacattt aaataatgtt tataaattat
    36181 attctaattt tagaaacatt ataatttcgt tgattaatta tagggttatt agaaatgaag
    36241 ggatttttag catattgacc tgacggcagc agtccgccag gtcagcgtcg taatattatg
    36301 ccgtccgggc aacggcatca cgcgaagcgg catcgcgctc ttcgcccgtc agctcgctaa
    36361 gttgcccatt gcgcatttcc agcaggcggt cggcgtggat aaagtaatga tcatcatgac
    36421 tgatagcgaa aatagtttta cccatctcct gcatcagcgg cagcaacacc tgataaaact
    36481 cacgacggaa gtgtggatcc tgatccaccg cccattcatc cagcaggata atatcgcgtt
    36541 cttctgccag cgccagcaac agcgccacgc gttttttctg cccttttgat aacttcaggt
    36601 taacaatacg cccgttgctt aactcaagct tatgagccat tttcagctgc gccagccact
    36661 tctcaaccag ttgcgggtta gcgggttgcc cctccggccc cagcagttga tcaaacagcc
    36721 agacatcggt aaacactgcc gaaaacagtt tgcgataatc ttccggttgt tcgccgctga
    36781 caggtttgcc atccagcaag atttcgccgc tttgtggctg atacaagccc gtcaacaaca
    36841 tcgccagcgt cgattttccg ctaccgttac cgccaatcag aaacagcagc tcgccacgtt
    36901 tgatggtgag attaatcgga ccaacggaaa acgcgttatc ctgataagca aacgtcacgt
    36961 tacgcagctc cagcgtttgc cagttgggaa acgcctgcgg gcgcggaaac tcagctttga
    37021 aaggcgcgag cgcgaatttg ttcagcttgt taaacgccac ctgcgccgtc agcagcgtcg
    37081 gcaatgcgcc aaccgccgaa agcagcggcg tacgcaggaa taacagcgtc aacgaatagg
    37141 tcgcagcaac gttggtatca gcccaaccga gactgttcgc catccagaac accaggccga
    37201 tagcgcccag catcatgatg tttgaccagt tcacggcact aagatggaag gtgtctgcgc
    37261 gaataatatg atggcgatac tcttgcgcat cggggatgta gaggttgtta aacacatact
    37321 cggcgcgttc ccggttcaga gtcagctctt tgcgcccttc aagtacagtt tgaaaatccg
    37381 tgtacagctt gtcttcggtt tcacgtaggg tcgccatatg tttgtacacc cgcgccacca
    37441 gcacaaaacc gccccagatg gtgatcgcca tccagatagc cgttaccagc aacattttgc
    37501 ccgacagcat ccacagatac gccgatgaac cgatagtgag aatgatcccc tgccccagtt
    37561 ccggcagacg cacaaaagca atggtgatat tgcgcacatc gctggttaat cccgccagca
    37621 acgaggcgct accgagttgt tcaatgcgct cgacgtgggt atccagaatc cgcttgataa
    37681 attcactacg cagtcggtaa acgaagtgat gccccaaagt ggtgagcgcc agttgcgatc
    37741 cgagagtgac tgccatcaac agcagcaata atcccagaaa ctccggtaac accagcagac
    37801 tggtatccgc cgtttcgata aggcgctgat tgataaaagc aattaagcca atgcctaatg
    37861 ccgcactggc gaggcttagc gccatcacac tgataaatgg ccagcgatac tgccgccaga
    37921 caagtacaag aagttccatg cagaaaaccc ggacaatgaa ttacagcccg cagtttaaac
    37981 atcttcgcgc gcacagcaat aataattctt atttttattc ttttttacct gcctgacgga
    38041 atgtcaggtt gtagcggcag tcggtggtga gtggatgaaa ccccgctttc aacggttgaa
    38101 taccgtgata aaacagccgc gattcaccgc cccataccac cacatcgcca tgttccaaca
    38161 acaaacgttt gagcggatca ttacgtttca ggccaccaaa ttgaaaaatc gcgggtaagc
    38221 ccagagaaac agaaacaatt ggcgcgcgca gatccggttc gtctttatcc tgatgcagcg
    38281 acagtttcgc gccaggagcg tagcggttga taagacaagc atctggctgg aaatctggat
    38341 agcccgccgc cgtagccgca cgttgacata aatcatgaaa gctttgtggc atagcgggcc
    38401 acggtttatt tgtttgcgga tcaatgggcg aatagagata accttgccga tgggtcgtcc
    38461 agcccagatg cccacagttg gtcatcgcca ccgacatggt atatccgcct ggagcgacca
    38521 tctggcgaaa cggcgactgg ctggcaacgt cattaatatc gcggattagt tgctccgcag
    38581 cattaaaagc aaaacgccgt aaaattaccg caccagccgc cagtggctct tgccacggtt
    38641 cagcatcggc aaacagatcc aacattacct ctcctcattt tcagcttcgc ggcgcagcag
    38701 ttgcgcttta cgcgacacgc cccagcggta accggaaagt gtgccatcac cacggaccac
    38761 ccgatgacag ggtattacga tagccagctt gttggcggca caggcgctgg caacggcccg
    38821 taccgctttc ggtttgccga tggcgttagc cagttgctga taactgacgg tttcaccaca
    38881 aggtatcgtg cgcagtgcct gccagacttg ttgctgaaaa gcagtgccgc gaatatccag
    38941 cggtaacgtc agcggcgtat cgcgttgatt gaggctggcg atcacttcac gcacatgttg
    39001 ctgaaacgtc agatcggcag gcgcgttgtc ggcagcggga aacatctgct gcaactcgct
    39061 gattaatgtc gcgtcatcat cgcccagcaa tatcgcgcaa atcccccgct cgctttctgc
    39121 caccaggcaa cgacccagct cacaatcagc cagcgcgtaa cgcaccgcca gattttcgcc
    39181 accgtgacgg aattgtttag ccgtcatgcc cagcgtttca tcggctttgc ggtagtaact
    39241 gctgctgtcg gggaatccgg cgttaagaat agacgtcgtc acgctctccc ctttcgccag
    39301 cgattcgcgc aaacggcgag cgcgccaggc ctgttgccag gctttaggcg tcattccggt
    39361 agtagcttta aacaaccgat gtagatgaaa tggactcatc gccacctggt cggctaaggc
    39421 ttccagcgtt acaggcgttt cctgttccag cagtcgacac gcgtgggtga ttttatccaa
    39481 ccgatgttgc cggggattgg ctttgtctgg ctgacaacgt ttgcaggggc gaaatccggc
    39541 ggcgagtgcc tcgctggcat ttgcgtagaa ggagacattt tcccgcaagg catgtctggc
    39601 gcggcaagaa ggacggcaaa agatacccgt ggtgcgcacg gcgaaaacga attcgccgtc
    39661 ggcattcggg tcgcgggcta agacagattg ccagcgttga tcgtcagtta agcatgtggc
    39721 gtttttcata atcagctccc tggttaagga tagcctttag tctgcccggt caccatcacg
    39781 caaaaaccaa caatcttgcg ctttaatttt tttcgctgac aaggaagctt ttaaactgtg
    39841 gtgacatcca ggttttaaag ctatcgcctt ctttggtgat catataaacc gccagccctt
    39901 cccggcgaac aacctctttg gctttctccg gcccgagtac catcaagcca gtatcccagg
    39961 catcggcttc cagcgccgtc ggagcaatca ccgtcacgga taccagattg tgttcgatgg
    40021 gacgcccggt ttgcggatcg ataacatggg aaagacgttt gccgtccagt tcgtaatagt
    40081 tacggtagct gccagaggtg ctaatcccat gaccgttgat atccaccaca gcctgaaccg
    40141 cgttttcttt atcggttggt ttttgaatcg ctacccgcca cggctggcct tcaccgttca
    40201 taccacggct gttcagcgcg ccgcccaccg acaccagata gcgggaaatc ccttcctgct
    40261 ccatcaagcg tgccaggtga tccgccgcat aaccttcacc gacggtggag agatcgacat
    40321 ataaatccgg caggtctttt tgcagatatt gctgatgcga ctgattgatg accgtcaggt
    40381 gctgtaagcc ggttttggct ttcatcgcat cgatctgttc ctggctcgga atttgaaccg
    40441 gctgttgttc cgggccaaag ccccacagat tcaccagcgg cccgacggta atatccatcg
    40501 caccatcggt ccttgcccca atgcgcagcg aggtggtgac gatatcggcc atcgcttcac
    40561 ttaccggcca cggcgacaaa ctttgcgagt cgttaaagcg catcagcgcg gaatcttttt
    40621 tataggtcga aagcagctga tcgtcggcgt ccagctgggt ctgaatcttt tctttaagtt
    40681 cggcactgcg tttggcatca atgcccggga tgctggcacg ccagaaggta cccatggttt
    40741 tgccttcaag aacggtaact tcagtagcgt gggttttggc gggttgtggt ttttgatcgc
    40801 aaccaacaaa gaagagcgcg gcaaccagca gtgccacgcg ggtaaagctt atttccatac
    40861 gtgattatcc tcatgcgaac ggtcgcaaga gtacaccaaa aaactgtgtt tgtacgctga
    40921 aaacaatgaa aaaagggccc gcaggccctt tgttcgatat ccatcgagat tagaactggt
    40981 aaaccagacc cagagctacg atgttatcag tgttgatgcc agcgtcacga gtgaactggt
    41041 tgtcgtccag caggttgatt ttgtagtcaa cgtaggtgga catgtttttg ttgaagtagt
    41101 aggtcgcacc aacatcaaca tatttcagga tatcttcgtc gtcgtagtta cgaccattga
    41161 tgacacccag gtttttacct ttagactgca ggtaagccag ggacggacgc agaccgaagt
    41221 cgaactggta ctgagcaaca gcttcgaagt tctgtgcttt gttcgcccaa cccaagttgc
    41281 ctacgcgagt tgcgttgtag gtctgggtgt actgagcagc taggtagatg ttgttcgcgt
    41341 cgtatttcag accaccggtg taggtttcag cgcggtcgcc attaccgatg taacgctcat
    41401 cataacggtt cagaccgaag ttctggtcat cagtacgttt ggagctggaa actgcagcac
    41461 cgataccgaa gccttcgtaa tcataagtaa tagaaccgcc aacgccgtca ccgttctgac
    41521 gcagagcttc acgaccgttg ttggtcatgc cttcaccttc cgggctaccg tttttgccct
    41581 gatactgaac agcgaagttc agaccgtcaa ccagaccaaa gaagtcggtg ctacggtagg
    41641 ttgcgaagcc gttaccacgc tgctgcatga agttgtcaga accgtaagtg tcaccaccga
    41701 attctggcag tacgtcggtc caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt
    41761 agtcgaaaga acctacatcc tggaatttca gacctgcgaa tgccacacgg gtccaggagt
    41821 tgttttcgtt ttcagcgctg ttgccctgga tctgatattc ccactggccg taaccggtca
    41881 gctggtcagt aacctgagtt tcacctttga agccaagacg catgtaggtc tggtcgccat
    41941 ctacagactt gtcgtcagag aaatagtgca ggccgtctac tttaccgtac agatctaatt
    42001 tgttgccgtc tttgttgtaa acttcagcag cgtttgctgc gcctgctacc agcagagctg
    42061 ggaccaggag ggacagtact ttaactttca tgttattaac cctctgttat atgcctttat
    42121 ttgctttttt atgccactgc atactgatta accctcatta atcagtcggc aagtccattc
    42181 tccccaaaaa tgcagaataa tccaacacga atatgatact aaaactttta agatgtttca
    42241 tttatcgcta tagatgtttc aaaatgtaaa tacaagggaa ctttttaaga ttattgcgga
    42301 atggcgaaat aagcacctaa catcaagcaa taataattca aggttaaaat caataactta
    42361 ttcttaagta tttgacagca ctgaatgtca aaacaaaacc ttcactcgca actagaataa
    42421 ctcccgctat catcattaac tttatttatt accgtcattc atttctgaat gtctgtttac
    42481 ccctatttca accggatgcc tcgcattcgg tttttttacc cttctttaca cacttttcat
    42541 tattctgtgc taccacagaa aaactataac gcttgttaac tatttcacaa ataattaaca
    42601 tcagcataat ttccagcaat ctttgtttat ttgcaattaa tttcgttggg ctttttgtag
    42661 gttatttgta cagcaaaatg gcgcttgtac atctatttcc ccccatacag gatgataaat
    42721 atcacgggag aatagagaat catcaatcag gtaagagtct ggaatttcac actgtaccct
    42781 ttatactgcc ctatcacttc gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa
    42841 gagacaacgg ccacgacccg cttttcactc ctaccgggga gcattacccg cttcttttta
    42901 ctgttgatca ttgtgttact ggtgacgatg ggggtaatgg tacaaagtgc cgttaacgct
    42961 tggctgaaag ataaaagtta ccagattgtc gacattaccc acgcaatcca aaagcgcgtc
    43021 gatacctggc gttacgtgac caggcagatc tacgacaaca ttgccgcgac gacctccccc
    43081 tcctccggcg aaggtttaca agagacgcgc ctgaaacagg atgtctacta tctggagaaa
    43141 ccacgccgca aaacggaagc gttaatcttt ggctctcacg acaactcaac gcttgagatg
    43201 actcagcgga tgtccactta tctggataca ttgtggggtg cagaaaatgt accgtggtcg
    43261 atgtattacc tgaatggtca ggataacagt ctggtgctga tctcaaccct accccttaaa
    43321 gatctcacct ccggatttaa agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca
    43381 gaaatgttac aacaggccaa cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc
    43441 ctagcctggc agaacggtca ttactttacc ttgcgtacca cgttcaacca gccgggacat
    43501 ctggcaacgg tcgtggcttt tgatctgccg attaatgatt tgatcccacc gggtatgccg
    43561 ctggacagtt tccgccttga gccagacgcg acggcaacgg gaaacaatga taatgagaaa
    43621 gaagggacgg atagcgtcag tatccacttt aacagtacga agattgaaat ctcctcggca
    43681 ctcaactcta ccgatatgcg cctggtctgg caggttcctt atggcacctt attgctggat
    43741 acgttgcaaa acattctgct gccactgctg ctgaacatcg gtttgctggc gctggcgtta
    43801 tttggctata ccacattccg ccatttctcc agccgcagta cagaaagcgt ccccagcacg
    43861 gcggtcaata acgaattgcg cattttacgg gcaatcaatg aagagatagt ctcactgttg
    43921 ccgctcggcc tgctggttca cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt
    43981 gccgatcatt tgctgccgca tctgaatctg caaaacatca ccaccatggc ggaacagcat
    44041 caggggatta ttcaggcgac gatcaataac gagctgtatg agatccgcat gttccgcagc
    44101 caggtcgcgc cgcgcacaca aattttcatt attcgcgatc aggatcgcga agtgctggta
    44161 aacaagaaac tcaagcaggc gcagcgtctg tatgagaaaa accagcaggg gcggatgacc
    44221 tttatgaaaa acattggcga tgcgctgaaa gaacccgcac agtccctggc ggagagtgcg
    44281 gctaaactca acgccccgga aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg
    44341 ctggtcgaag aaatacagtt agcgaacatg cttgcggatg atagctggaa aagtgagacg
    44401 gtgctgttct ccgtgcagga tttaattgat gaagttgtgc cttcagtgtt gcctgccatc
    44461 aagcgtaaag gtctgcaact gctgattaac aatcatctga aagcacacga tatgcgccgc
    44521 ggcgatcgcg atgccttacg acgtattttg ctgctactga tgcaatatgc cgtgacctca
    44581 acgcaattgg gaaaaatcac ccttgaggtt gatcaggatg agtcctctga agaccgcctg
    44641 acattccgca ttctggacac gggagaaggc gtaagtattc atgaaatgga taatttgcac
    44701 ttcccgttta tcaaccagac ccaaaacgat cgttatggaa aggcggaccc gctggcattc
    44761 tggctgagcg atcaactggc acgtaaactg ggcggtcatt taaacatcaa aacgcgggat
    44821 gggcttggta cacgctactc tgtgcatatc aaaatgctcg ccgctgaccc ggaagttgaa
    44881 gaggaagaag agcgtttact ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa
    44941 attcggaata ttgtcactcg cctgttagaa aattggggtg caacctgtat cacacccgat
    45001 gaaagattaa ttagtcaaga ttatgatatc tttttaacgg ataatccgtc taatcttact
    45061 gcctctggct tgcttttaag cgatgatgag tctggcgtac gagaaattgg gcctggtcaa
    45121 ttgtgcgtca acttcaatat gagcaacgct atgcaggaag cggtcttaca attaattgaa
    45181 gtgcaactgg cacaggaaga ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa
    45241 ctccatgcca gcggctatta tgcgctcttt gtagacacag taccggatga tgttaagagg
    45301 ctgtatactg aagcagcaac cagtgacttt gctgcgttag cccaaacggc tcatcgtctt
    45361 aaaggcgtat ttgccatgct aaatctggta cccggcaagc agttatgtga aacgctggaa
    45421 catctgattc gtgagaagga tgttccagga atagaaaaat acatcagcga cattgacagt
    45481 tatgtcaaga gcttgctgta gcaaggtagc ctattacatg aacaatatga acgtaattat
    45541 tgccgatgac catccgatag tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg
    45601 ggtgaatgtt gtcggcgaat ttgaagactc tacagcactg atcaacaacc tgccgaaact
    45661 ggatgcgcat gtgttgatta ccgatctctc catgcctggc gataagtacg gcgatggcat
    45721 taccttaatc aagtacatca agcgccattt cccaagcctg tcgatcattg ttctgactat
    45781 gaacaacaac ccggcgattc ttagtgcggt attggatctg gatatcgaag ggatcgtgct
    45841 gaaacaaggt gcaccgaccg atctgccgaa agctctcgcc gcgctgcaga aagggaagaa
    45901 atttaccccg gaaagcgttt ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga
    45961 caagcgtctc tcgccaaaag agagtgaagt tctgcgcctg tttgcggaag gcttcctagt
    46021 gaccgagatc gctaaaaagc tgaaccgcag tattaaaacc atcagtagcc agaagaaatc
    46081 tgcgatgatg aagctgggcg tcgagaacga tatcgccctg ctgaattatc tctcttctgt
    46141 gaccttaagt ccggcagata aagactaatc atctgtaggc cagataagac gcgttagtgt
    46201 cttatctggc atttgcaccg attgccggat gcggcgtaaa cgccttatcc ggcctacgat
    46261 tcccattatt tcaacagatt acattaaagt aggccagata agacgcgtca gtgtcttatc
    46321 tggcatttgc actgaatgcc ggatgcggcg taaacgcctt atccgtccta cgaatcccgc
    46381 gatttcctga ccctctcggc atataccgtc agcgtctgtt ttatcacatc cagcgttacc
    46441 ggcttcgaga ggcagctgtc cataccggac tccagacacc gctgcttctc ttcagccaac
    46501 gcattagcag ttactccgat taccggcaac gtcagtccca actgacgaat gcgttgcgtc
    46561 aagcggtaac catccatatt tggcatgttg acgtcgctaa gcacgatatc aatatgattc
    46621 ttactaagta cattaagcgc atcgacgcca tcattcgcgg ttttacattg atagcccaac
    46681 gatcccaact gatctgccag cagacgtcgg ttaatcggat gatcatccac gaccagaatc
    46741 atcatatcgt cgttatcgct gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg
    46801 tcgctctcca tctcgatcaa ataaatacgc gccaacaatg ccggtagctc atgcggagca
    46861 gccacactgt gtacccactc ccctggcgct ttctccagcg gaataccaat atggcgacga
    46921 cagaaggtca ctaccgctct gccctgccat tttttactca ctacctcgtc agtgattaac
    46981 acatcttcgg gagtcggttc ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc
    47041 aaactggttt ccaggaactg acagagcgac gcattgcgga ccgccagcca gcagcgttta
    47101 ccactcaacc cttccacgcc ttttttctgc gggtactgag cgccgtacaa cggaatacgc
    47161 acggtaaact ggctgcccat tcccggttct gaatctaccg agatatcgcc gtccatcatg
    47221 ctgatcagtt tttcacaaat cgccagaccc agaccggtcc cctggaagtt tcgctgtacg
    47281 cccgttccaa cctggaagaa gggatcaaac aagcgcacca cttctttcgc cggaatcccc
    47341 acgccggtat cgcgaacacg gatagagaga taatcgccat cagcgcgaac atgcaaaact
    47401 atacagccgg tatcggtgaa ttttatggcg ttactcaaca ggttggagat gacctgctgt
    47461 aaacgcatcg ggtcgccatt taaggccact ggcacatccg gttcaataaa gcagtacaag
    47521 cctaactgct tgcgtaccac cagcggtaaa tagttggcgg tgatgtggtt catcacttca
    47581 cgcggtgaaa actcacgcgg ttcgatcttc aactgttccg attcaatctt cgagaaatcg
    47641 agaatatcgc tgataatttt caacaacagg ctggaagagt tgttcattgc cgtcaccaga
    47701 cgatcgacgc ctttcggtaa ctctttggtt tgcaacagat ccaggttacc gataatgcca
    47761 tacagcggcg ttcgcagctc atgactgacg gtggcaagga acatcgattt tgactggctc
    47821 gcctgttccg ctgcttgtgc catctcctgc aacgactctt ccatcttcac gcgcgaagaa
    47881 acatccacca gcacacaaat ggccacgttt tcattacgat agcgcgaatg gacgaagctg
    47941 atttgcagat tggtattgtt gctggtcagg acatcaacaa aattgacctg ctgcccacag
    48001 ataatttgcg tcagtcgttg gcggtcctca tgcgtaagca tattgagata ggtatgcgcc
    48061 agttcgttac ttaaaatatt gacgccatca gcggtacgca aaatgcagat acccactggc
    48121 gcggaggcga caatcttgcg attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt
    48181 tccgccggaa tgaaaatacg tcgctcgtac atccgcgcga gagtaaacaa tgcagctccg
    48241 gcaagcacat tcagcaaaat tgcgttaagg atcaacatgc gaatgcgttc cagcacctta
    48301 tcaaccggca ccgaatacac gatgcttagc gatgagggtg gcagattttt cttcagcacc
    48361 agctcccgga acccttccgt atagccaaac caggagcgtt cctgcatcca gcgaggatcg
    48421 cccttaattt tactttctgg tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga
    48481 atggtaaccc ccatcggcaa cgtacccggt aagaaaaagt tctccatccg gatggtctgc
    48541 tcgacaccca aaagcgcctg caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa
    48601 tacccgacgc aagggcgcgg accttcgctg atccagtaga ggttactgcc gctatcatct
    48661 tgtggtgcat ttcgatattt attgatgcgt tcatgcaacg ctttcaacgc ggtatcgcgt
    48721 tccactggca tatcacgcag accgaaattg gccatgcaga ggttatcact gccgattaaa
    48781 aatacccggt tgagatcgta agccgcagaa aaattatcgc gccagtagcg cataaaccac
    48841 gccaatgact ccagagaacc tcgccaggtg ttactcattg cggaacaatc ggagtcggca
    48901 aacagcggtt caaacgcagg cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca
    48961 ccgttttctg ccgataagcg attttcggcg atgtacttaa gctctttcat cacatcagag
    49021 gtgcgttgaa taaagcgctg agcctgatcg gaactcagat taaattcctg acgaatttcc
    49081 gattctcgct gatgtaacgc attaacgatg taaaaaacgg atgaaaaagc aatcaacagc
    49141 cagagcacta acgccaatgc tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa
    49201 gaagcaaggt atttcaaagg ggagaagctc cgcctcaggt gaccgatgga gtgtggttaa
    49261 ggtagcggta aaagcgtgtt accgcaatgt tctgtcacca ttgcaataat gcgtatcgct
    49321 tagaaaccat tgccttaacc aggaaagccg gagtagatgg acagttctca gtacgaaaaa
    49381 taagaaatga ccaatctgcc gacgcttgtt aatggttata tcctggcgtc ggcagatcgc
    49441 ccagcagtct tatctgaaaa aagagagagc caggttgtgt gaagggatgg atcaccagtc
    49501 aaaacttttc accagcgtca gctcgccagc attacgcatc ggtacaataa atgtttcctg
    49561 tttctcattg accgatcctt catcggtgat cagcgtcagt tgggcggtcg tcaattccgt
    49621 ttcgctgcgc ccaccatagt agttgatata cacctgatag cgcccgtgaa ctggtgctgg
    49681 catggcgaaa atctcgggtc cgtaccccgt cgtgacatcc atatccagtg caccactgtt
    49741 tttcagcacg gtgttaccgt accaggcgtg ttcgccatcg ggcgtgacaa catggagatc
    49801 gagatcggta ttgtccgtat cccacgagag aaccagccgt aaacgtgcac gaatagttcc
    49861 tgtacccggc gttgagtaaa actgcatttt ttgccggctt tgcccatccg ggctgatgac
    49921 ctgcacgctg ttgctgcctt cagtgaaaat ataagggcgt gcaaatgaac catcggattc
    49981 aattctctgc ggcatactgg caccgttgac caccagccgg ccctgctgaa ctttacccgc
    50041 cgcagcataa ttctttattt tgccgcggat ctgcgctgag atattttgat catctgccat
    50101 gttgacggac gacgccgggt aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc
    50161 cggatgccag ccagagagcg gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc
    50221 gacaggcgaa agtgccacca gtaacaacgg aagaaaaatc tttcgcatag cattagtcca
    50281 ttaataattg tcgggtcagc gtttcgatat agttctcatc aagcccggtg ggataaccat
    50341 caaaggccaa gtgcagatac tcatgggtta aatccagccg atcctgaagc gtaaagaagt
    50401 tacgaataaa cagccgtttc tgctgccgat cggtataggg gaaacctgag actaaacgac
    50461 agaccgcaaa cacgtctggt tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact
    50521 gcggcatttt tttcgccagc caggctttcg ctttgggtaa taactggcag gttgagcgtg
    50581 gagctcccca gcggctaagg ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac
    50641 gctccccctg ccccgcctgg gccgtggctt gccgccagga aagtgtccct tcggtagcgc
    50701 gactgccatg ataatgaacc ggatcgcctg catagatgag ctcctgcgtc caggcggtca
    50761 tcgttcgcgc ccccgtggtc gctggcgaag cagaaacacg ctgcgtggcg ctgctgtcgg
    50821 ggatggtcag gcaatcacct tcccgattgg cgttttgctg taaaaaagta cgtatcgcca
    50881 cagtcatggc tttcgctgcc tcaggtggcg tacttttcgc ctcgcgatcc agaactctgg
    50941 caacgtactc ttcacgatcg agatgcgact gcaatttcag tttgcccttt tcgctcagta
    51001 aggtcgtctc accgtggctg acaaacgtaa tgtggttacc attagtaaac gtcacacgat
    51061 agcgcccatt aagtacgctg ggttttactg ccgtcgtact cttttccgct gtgattttct
    51121 tcagcggata gcgggcaaac agctccacct ctacacattg cccactggca acctgcgtcg
    51181 gcactggcaa aacccgatta agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc
    51241 cactgccagt aacccacagc ggtgtaccat cggttaacca gccagcaaaa ccaccctgac
    51301 ggatttcctg tttatcatcg gcaaaccagc tccaggtttt cacccgtacc cgactaccga
    51361 gcatggatgc gacaccgatt ttcgcctcgt ccagcaccac atccagtaag acttcctgag
    51421 ctttgttttg cgcaggtaac gtcgctaatg aatcaagcag cgatttaacc gttaccgagg
    51481 tttctggctt cagcgttgta agagacgcta accacgcagg tgcctgacgc tgttgccagt
    51541 attgccccca cacatctgcg ccaatatgca accgctgcgg cgcaaaatag agcccacagg
    51601 aacgtaccag cgcggtatcg cgcgttatgc tctccccagc ctgacaacaa taaacctctt
    51661 ccggggagtt tccgcgacac tggtagggct gctccggttg atgggtatcc tccagccagg
    51721 cgtaaacata cagtttccac aaactcccta acggcgtggt gagcgagtca ggtaatgctg
    51781 agactttggt cacgcctgat gacgacagct gatacagttg atcgtgctgc gcaccgcgca
    51841 gcaccagttg taacggcgtc tcctccgcaa gcgttggcag agtaaccaac gccaatagcc
    51901 agacgattct gcgccagttc acttatttca cctgcatccc ggtccattca ctccctgccg
    51961 cgacactttg ctgcgcaggt gcataggaac gcacataacg agcaggaggc agaacgaatt
    52021 gccctttttg cgagaagcgc agcaaatggc ggaaagtgac cgttcccgtc agttctttca
    52081 ccggcaccat ataggccagt tcgcccattt cattacgcgc tttttccagc aattgcccct
    52141 gctgtttcgc ggcgttgggt ttattgactg aaatgcccca tgttgtgcgc tcaacgtcag
    52201 ctcccggcgg gagcggtact tccacctgac catagcgcag aactgcatcc tgctcgctgg
    52261 taagcgtaat ttcatcgaga tacagcgcat cgctgtcaat ctcattgctg gtcaccggct
    52321 gcagagtaaa gctcatctct tcttcaccgg ggataagccg atacaactgg cgttcaacgg
    52381 tcaccggaat gttactttgt tgagccgttt ttgccggttc acgccagcgg acctgcacat
    52441 ttgcggcgat aattcgtcac caaaagagag aatgtccggc acaccctaac caacccaacg
    52501 ccagtcttcg ccccctccag ttaacttatg tttagcccat gcgcccgcag gcgcaggcaa
    52561 aacaactgga ggcattgtcg ccatatattt cgccagccag ttcatggcca gcgcgcgctc
    52621 aatagtggat tgctcagcgg tcaaaccatt aaaatagcgg ccgtctgggt agcgtcacca
    52681 ccgctattga gcagcacaac cgtgtgcgcc agcggctgat tgctgtttgc cgctttatcc
    52741 cacgcatatt gctgaacctg tggcatcacc gtggacttca actgcgcttt tttcagcaac
    52801 gtcatggtta agacgtttgc caccgcatct gccagtggag attccggtgt atcgaggatc
    52861 aggctgtcat tgatatcgcg gatgtcttcc tcagagaaat cttcatcttt agttccgcgc
    52921 cgggcgatag cttcatcccc ccctttcaac aacgttttgc acggcagatt catctcctgt
    52981 gcccacgcca gcaccagcgc ccgatgcaat aacggcatat tatccgcctg ctcggcataa
    53041 ctgtcgagca tatgctgcca gtattccggt tgttgcgtta cgccgagcgc ctggctggcc
    53101 tgccagtcgg cgtaccatgc ccatgccgta aggaaggcgt caccattgcc atcttcaccc
    53161 caccaggtaa agcgcgctcc gggccccgcc agttgcatca gccgcagacg gttatcctga
    53221 atcatctgac gaatgtcgtt agcggcggca ctttgatgat cggcaagcga acgccaggcg
    53281 agactgagcg ggatcagacg gctaccggtg ttgatcaccc caccccacgg ttcatcgact
    53341 aacgcatcaa gattgttgcg gaaaatctcc tgcggcgttt cactactttg tagccggata
    53401 ttactcgcct gctcgggcaa catcagcgcg ttctcgccac cgccgagcat gacatttttc
    53461 tgctgttcaa cgggccagct gttgtccaca aaagacagtt ttgtgctaat gctgtcctgc
    53521 acttgcccat tttgttgcag ttctgcactt aacaagccag attgctgaat attctgcgtc
    53581 agcgaaatat aattcgcccc tttgtgcagc gtcagcgtct ggcgcatctc agcgcctgca
    53641 aatttagtca ccagcgccac cggttcgtta tcctgctgac tgaagataaa cagtcctgcc
    53701 gccggtttgt cgcccacgcg atacaccgtt ggcatactcc acttcatgta gagatttttt
    53761 tccgaacgca gataagcacg cccctgcccg accagcccgt cgccgttcat cccacgcgcg
    53821 gtgatacgcc agcgggttaa cgaatcaggc atcaggaacg tgaaatacgc tttgccttgt
    53881 ttatcggttg tgagtgacgg catccatgcc gcggtatcca cctcttcacg ccgtggacgt
    53941 tcaagcattt ttactcgccg ctcgctgcgg ttagtcgcgc caggcgcaac cggctcgctg
    54001 gagagcgcct ggtcatagct gataaacgac agactggagc tggtacgcac attgttacgc
    54061 cccagcggat agaaaaattt gccgatattc ggcgcgattt ctggttgcag cgcgtagatc
    54121 atttcatcga ccacgcctac cgttagctgc gcagaaacag gtttaccttt cagcgacgag
    54181 gttaat
//
LOCUS       DAZPDE010000007        53180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPDE010000007 DAZPDE010000000
VERSION     DAZPDE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.007"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            339..425
                     /gene="leuL"
                     /locus_tag="ACHU0J_000360"
     CDS             339..425
                     /gene="leuL"
                     /locus_tag="ACHU0J_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1215208.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            518..2089
                     /gene="leuA"
                     /locus_tag="ACHU0J_000361"
     CDS             518..2089
                     /gene="leuA"
                     /locus_tag="ACHU0J_000361"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1215209.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2089..3180
                     /gene="leuB"
                     /locus_tag="ACHU0J_000362"
     CDS             2089..3180
                     /gene="leuB"
                     /locus_tag="ACHU0J_000362"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1215210.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3183..4583
                     /gene="leuC"
                     /locus_tag="ACHU0J_000363"
     CDS             3183..4583
                     /gene="leuC"
                     /locus_tag="ACHU0J_000363"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1215211.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4594..5199
                     /gene="leuD"
                     /locus_tag="ACHU0J_000364"
     CDS             4594..5199
                     /gene="leuD"
                     /locus_tag="ACHU0J_000364"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1215212.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5248..6426)
                     /gene="setA"
                     /locus_tag="ACHU0J_000365"
     CDS             complement(5248..6426)
                     /gene="setA"
                     /locus_tag="ACHU0J_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1215213.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6528..6659)
                     /gene="sgrT"
                     /locus_tag="ACHU0J_000366"
     CDS             complement(6528..6659)
                     /gene="sgrT"
                     /locus_tag="ACHU0J_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1215214.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6748..8403
                     /gene="sgrR"
                     /locus_tag="ACHU0J_000367"
     CDS             6748..8403
                     /gene="sgrR"
                     /locus_tag="ACHU0J_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1215215.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8437..8536
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8567..9550
                     /gene="thiB"
                     /locus_tag="ACHU0J_000368"
     CDS             8567..9550
                     /gene="thiB"
                     /locus_tag="ACHU0J_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1215216.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9526..11136
                     /gene="thiP"
                     /locus_tag="ACHU0J_000369"
     CDS             9526..11136
                     /gene="thiP"
                     /locus_tag="ACHU0J_000369"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1215217.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11120..11818
                     /gene="thiQ"
                     /locus_tag="ACHU0J_000370"
     CDS             11120..11818
                     /gene="thiQ"
                     /locus_tag="ACHU0J_000370"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1215218.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11932..12696)
                     /locus_tag="ACHU0J_000371"
     CDS             complement(11932..12696)
                     /locus_tag="ACHU0J_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1215219.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12782..13660)
                     /gene="araC"
                     /locus_tag="ACHU0J_000372"
     CDS             complement(12782..13660)
                     /gene="araC"
                     /locus_tag="ACHU0J_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1215220.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13999..15699
                     /gene="araB"
                     /locus_tag="ACHU0J_000373"
     CDS             13999..15699
                     /gene="araB"
                     /locus_tag="ACHU0J_000373"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1215221.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15710..17212
                     /gene="araA"
                     /locus_tag="ACHU0J_000374"
     CDS             15710..17212
                     /gene="araA"
                     /locus_tag="ACHU0J_000374"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1215222.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17412..18107
                     /gene="araD"
                     /locus_tag="ACHU0J_000375"
     CDS             17412..18107
                     /gene="araD"
                     /locus_tag="ACHU0J_000375"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1215223.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            18182..20533
                     /gene="polB"
                     /locus_tag="ACHU0J_000376"
     CDS             18182..20533
                     /gene="polB"
                     /locus_tag="ACHU0J_000376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1215224.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20698..23604
                     /gene="rapA"
                     /locus_tag="ACHU0J_000377"
     CDS             20698..23604
                     /gene="rapA"
                     /locus_tag="ACHU0J_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1215225.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23616..24275
                     /gene="rluA"
                     /locus_tag="ACHU0J_000378"
     CDS             23616..24275
                     /gene="rluA"
                     /locus_tag="ACHU0J_000378"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1215226.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24392..25207)
                     /gene="djlA"
                     /locus_tag="ACHU0J_000379"
     CDS             complement(24392..25207)
                     /gene="djlA"
                     /locus_tag="ACHU0J_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1215227.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25462..27816
                     /gene="lptD"
                     /locus_tag="ACHU0J_000380"
     CDS             25462..27816
                     /gene="lptD"
                     /locus_tag="ACHU0J_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1215228.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27869..29155
                     /gene="surA"
                     /locus_tag="ACHU0J_000381"
     CDS             27869..29155
                     /gene="surA"
                     /locus_tag="ACHU0J_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1215229.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29155..30144
                     /gene="pdxA"
                     /locus_tag="ACHU0J_000382"
     CDS             29155..30144
                     /gene="pdxA"
                     /locus_tag="ACHU0J_000382"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1215230.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30141..30962
                     /gene="rsmA"
                     /locus_tag="ACHU0J_000383"
     CDS             30141..30962
                     /gene="rsmA"
                     /locus_tag="ACHU0J_000383"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1215231.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30965..31342
                     /gene="apaG"
                     /locus_tag="ACHU0J_000384"
     CDS             30965..31342
                     /gene="apaG"
                     /locus_tag="ACHU0J_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1215232.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31349..32191
                     /gene="apaH"
                     /locus_tag="ACHU0J_000385"
     CDS             31349..32191
                     /gene="apaH"
                     /locus_tag="ACHU0J_000385"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1215233.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32269..32748)
                     /gene="folA"
                     /locus_tag="ACHU0J_000386"
     CDS             complement(32269..32748)
                     /gene="folA"
                     /locus_tag="ACHU0J_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1215234.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32939..34801)
                     /gene="kefC"
                     /locus_tag="ACHU0J_000387"
     CDS             complement(32939..34801)
                     /gene="kefC"
                     /locus_tag="ACHU0J_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1215235.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34794..35324)
                     /gene="kefF"
                     /locus_tag="ACHU0J_000388"
     CDS             complement(34794..35324)
                     /gene="kefF"
                     /locus_tag="ACHU0J_000388"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1215236.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35372..36766)
                     /locus_tag="ACHU0J_000389"
     CDS             complement(35372..36766)
                     /locus_tag="ACHU0J_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1215237.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36825..37112)
                     /gene="fixX"
                     /locus_tag="ACHU0J_000390"
     CDS             complement(36825..37112)
                     /gene="fixX"
                     /locus_tag="ACHU0J_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1215238.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37109..38395)
                     /locus_tag="ACHU0J_000391"
     CDS             complement(37109..38395)
                     /locus_tag="ACHU0J_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1215239.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38446..39387)
                     /locus_tag="ACHU0J_000392"
                     /pseudo
     CDS             complement(38446..39387)
                     /locus_tag="ACHU0J_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39402..40172)
                     /locus_tag="ACHU0J_000393"
     CDS             complement(39402..40172)
                     /locus_tag="ACHU0J_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1215240.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40528..42042
                     /gene="caiT"
                     /locus_tag="ACHU0J_000394"
     CDS             40528..42042
                     /gene="caiT"
                     /locus_tag="ACHU0J_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1215241.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42073..43215
                     /gene="caiA"
                     /locus_tag="ACHU0J_000395"
     CDS             42073..43215
                     /gene="caiA"
                     /locus_tag="ACHU0J_000395"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1215242.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43344..44561
                     /gene="caiB"
                     /locus_tag="ACHU0J_000396"
     CDS             43344..44561
                     /gene="caiB"
                     /locus_tag="ACHU0J_000396"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR1215243.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44635..46188
                     /gene="caiC"
                     /locus_tag="ACHU0J_000397"
     CDS             44635..46188
                     /gene="caiC"
                     /locus_tag="ACHU0J_000397"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1215244.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46297..47082
                     /gene="caiD"
                     /locus_tag="ACHU0J_000398"
     CDS             46297..47082
                     /gene="caiD"
                     /locus_tag="ACHU0J_000398"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1215245.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47088..47678
                     /gene="caiE"
                     /locus_tag="ACHU0J_000399"
     CDS             47088..47678
                     /gene="caiE"
                     /locus_tag="ACHU0J_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1215246.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47764..48159)
                     /gene="caiF"
                     /locus_tag="ACHU0J_000400"
     CDS             complement(47764..48159)
                     /gene="caiF"
                     /locus_tag="ACHU0J_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1215247.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48421..51642)
                     /gene="carB"
                     /locus_tag="ACHU0J_000401"
     CDS             complement(48421..51642)
                     /gene="carB"
                     /locus_tag="ACHU0J_000401"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1215248.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51660..52808)
                     /gene="carA"
                     /locus_tag="ACHU0J_000402"
     CDS             complement(51660..52808)
                     /gene="carA"
                     /locus_tag="ACHU0J_000402"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1215249.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT        12789 a        14453 c        13937 g        12001 t
ORIGIN      
        1 taattgttca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac atataattca
       61 atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga
      121 ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg tagttaagca
      181 gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta cggatgcaga
      241 actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt gtatccagta
      301 actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc gttcgcttta
      361 tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc ggcatccagc
      421 attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc
      481 gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag tcattatttt
      541 cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa
      601 actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag tcggtttccc
      661 cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg ttaaaaacag
      721 ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg ccgaatccct
      781 gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa tgcacatcgc
      841 caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata tggtgaaacg
      901 cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc gtacacccat
      961 tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca ccatcaacat
     1021 tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca gcggcctgta
     1081 tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg acgatttagg
     1141 cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg tggaaggtgc
     1201 aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca tcatggcgat
     1261 taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccaag agatatggcg
     1321 caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca aagccattgt
     1381 tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc tgaaaaaccg
     1441 cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc agctgaatct
     1501 gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg ggtataaaga
     1561 aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg acaaaaaagg
     1621 tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc aagaagagcc
     1681 ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata tcgccaccgc
     1741 cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc
     1801 ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg aactggtgaa
     1861 atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg atattgtcac
     1921 caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg tcgagtcctc
     1981 cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag tcgaaaaaga
     2041 gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat
     2101 taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac ccaggcgctg
     2161 aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag ccattacgat
     2221 gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac ggttgaaggt
     2281 tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg ggaacattta
     2341 ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca cttcaaatta
     2401 ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg tccgctgcgt
     2461 gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac cggcggcatc
     2521 tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc gtttgatacc
     2581 gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga atctgctcgt
     2641 aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc ctctatttta
     2701 tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact ggcgcatatg
     2761 tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga cgttctgctg
     2821 tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac tggctcgatg
     2881 ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga accggcgggc
     2941 ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca aatcctgtcg
     3001 ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc cattgaacgc
     3061 gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg tggcgctgcc
     3121 gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga aggggtgtaa
     3181 tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac gaagccgaaa
     3241 acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc tcaccgcagg
     3301 cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa accttcgcca
     3361 ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt gaaatggcgc
     3421 gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag ctgtatgacc
     3481 tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc gtcaccttgc
     3541 cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg tttggcgcac
     3601 tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa accctgaaac
     3661 agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg ggcattaccg
     3721 caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc accgggcatg
     3781 tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt atgaccctgt
     3841 gcaatatggc gatcgaaatg ggcgcaaaag ccgctctggt tgctcctgac gaaaccacct
     3901 ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac gacgccgttg
     3961 cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt gtcactctgc
     4021 aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag gtgatttctg
     4081 tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt gcgtcggcag
     4141 aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa gtggctatcg
     4201 acaaagtgtt tatcggttcc tgtaccaact ctcgcattga agatttacgc gcggcagcgg
     4261 agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt cccggctctg
     4321 gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt gaagccggtt
     4381 ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc
     4441 cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag gggcgcggcg
     4501 ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg
     4561 ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat caaacacaca
     4621 ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat cccgaaacag
     4681 tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt
     4741 ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc gcagtatcag
     4801 ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg tgagcacgcg
     4861 ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt tgctgacatc
     4921 ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga tgcagaagtg
     4981 gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt ggatctggaa
     5041 gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc cttccgccgc
     5101 cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga cgacgctatt
     5161 gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc ggtcaaatga
     5221 ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg ttaaaaataa
     5281 tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc aactttgtgc
     5341 aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt tggtaaataa
     5401 ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca tcccaatacc
     5461 cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg ccatacggct
     5521 atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa tgaccatcat
     5581 tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg gtatttccag
     5641 tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta aatcgctact
     5701 gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta acgtcgaggc
     5761 gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata aagcattttc
     5821 cgacggcagt tctacccgcg ccacagacag aagcataaat gcaatcaata ccagactgag
     5881 tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca acgccagcat
     5941 aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca tcaccgagct
     6001 aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag caaataactg
     6061 tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa gcgtcagata
     6121 atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc aaaatataat
     6181 cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc ttacgccgat
     6241 cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg cgccaacctc
     6301 acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc ccatcataaa
     6361 agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg tcattatcca
     6421 gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt ataatctgct
     6481 ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca actttcagaa
     6541 ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg gacgcttaac
     6601 caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa ctgacgcatg
     6661 gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa ataaaattgc
     6721 tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca acagttcatc
     6781 cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga actggcagcg
     6841 ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca ggatcgcggc
     6901 tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac attcctctat
     6961 accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga tcgtatcgac
     7021 caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt ttctcatctg
     7081 ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg tccgttgcgt
     7141 aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg gcaaatcttc
     7201 agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat cgcccaccac
     7261 tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt ccattttcat
     7321 catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat caatacgctg
     7381 ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct ggatatccat
     7441 ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc gatgatcctg
     7501 ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac cggtccgtat
     7561 gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga cttcttcggt
     7621 taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc cgacgagcca
     7681 gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga aagccgcctg
     7741 gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc gaatcagcaa
     7801 gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt cgctgaggaa
     7861 cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca ccatgcccgc
     7921 accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt ttatcaggat
     7981 cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag tcaccaggtc
     8041 acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat cgagagcgat
     8101 atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt cgcgcacttg
     8161 tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga cgccgcccgc
     8221 tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc cagtaaagca
     8281 atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat gcgcggcctg
     8341 cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc accggatcca
     8401 tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg gtgccacgca
     8461 aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg cgaagggatt
     8521 tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt taaaaaaatg
     8581 tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc tgactgttta
     8641 tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa aagcttttga
     8701 agccaactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt cgcttctcaa
     8761 ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc tggataacaa
     8821 tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg cagcggatgc
     8881 cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt acggctattt
     8941 cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga aagaattggt
     9001 tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca cgccggggct
     9061 gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag cctggcaaaa
     9121 actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg gcctgttttt
     9181 aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc acattctcga
     9241 agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc tgcaggtgga
     9301 agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat tcctccagtt
     9361 tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt atccggtggc
     9421 aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca cgctggagtt
     9481 cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc aacgcgccgt
     9541 cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct ggtggtagcg
     9601 gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa ctgggtggca
     9661 gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca ggcgtttctc
     9721 tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta tcgcaggcgc
     9781 tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct cccggtgcta
     9841 gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc atcgctctgc
     9901 caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat tttgctggcg
     9961 cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact ggaaaacatt
    10021 cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca ttttttccgc
    10081 ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct tatctttatg
    10141 ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca ggcaaccact
    10201 atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg cgcggcgatg
    10261 ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca gcgactaagt
    10321 aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga cgatcgtctg
    10381 catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt gctgccaccg
    10441 ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct ggcacaaccg
    10501 gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg tgtattgtgc
    10561 gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg gcagaaaatg
    10621 ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc ggggattgtg
    10681 ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca atctgctgac
    10741 ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa agtactggaa
    10801 aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct gggcattgaa
    10861 ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact ggcgcaggcg
    10921 ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt gttcggtaac
    10981 gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta tcgcagccag
    11041 gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt taccgtgatt
    11101 gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt atcaccattt
    11161 gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc tcgggccaag
    11221 cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc cagccagcgg
    11281 ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc gtccggtgtc
    11341 gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga acatcgggct
    11401 ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc acgcgattgc
    11461 ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt ccggcggtca
    11521 gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt tattgctcga
    11581 tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc tggtgagcac
    11641 gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg aagatgcggc
    11701 gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc agggtaagac
    11761 tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta cgggttagta
    11821 tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg ttttgtaggc
    11881 ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc cctaaacccc
    11941 aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa ttaacaccac
    12001 cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac ggccgcgcga
    12061 caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc agcacagcca
    12121 gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact cgccgctctg
    12181 cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga agtaaaacgg
    12241 tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg gcagatccag
    12301 cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac cgaccagaat
    12361 ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg ctttgttttt
    12421 cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc agaaggagat
    12481 ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga aacttaactc
    12541 gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta gaatcaaacc
    12601 gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg aatacacggt
    12661 agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa atggacgaag
    12721 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca
    12781 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
    12841 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
    12901 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
    12961 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
    13021 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
    13081 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga ttatccatcg
    13141 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
    13201 tcatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
    13261 acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc gtattggcaa
    13321 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
    13381 ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct cctggcggga
    13441 acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc accaccccct
    13501 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
    13561 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
    13621 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
    13681 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
    13741 ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg taacaaagca
    13801 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
    13861 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
    13921 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
    13981 tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag tgattctgtg
    14041 cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt agagtggtat
    14101 ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg tcatcatccg
    14161 cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga gcttagcgtc
    14221 gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac gcccgcaccg
    14281 attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg
    14341 atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat tacccgtttg
    14401 tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta ttccagcgaa
    14461 tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc gcaatctgcc
    14521 gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac cacccgcccg
    14581 caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg
    14641 ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa tcgccatttg
    14701 ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac cttatgcccg
    14761 gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac
    14821 tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa agttatcggt
    14881 acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg ggcagttaaa
    14941 ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc
    15001 caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg gccgctggaa
    15061 cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca gaaacaactg
    15121 cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct gccggtggtg
    15181 ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa aggggtgatt
    15241 accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat tgctgccacc
    15301 gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc cgtcaataac
    15361 gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac
    15421 gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg
    15481 atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca gcaaaaaatg
    15541 gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg ctttgaacag
    15601 ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct tccaacttcc
    15661 gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa tggcgatttt
    15721 tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc cggaaaccct
    15781 gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg aagcgaaact
    15841 gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca ccgctatttg
    15901 ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc acaccttctc
    15961 cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc tgcaattcca
    16021 cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta tgaacctgaa
    16081 ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc gtcagcaaca
    16141 tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg gctcctggat
    16201 gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat ttggcgataa
    16261 catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca agttcggttt
    16321 ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg gtgaactcca tcagcgacgg
    16381 cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc ctgccacaca
    16441 aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc tggggatgaa
    16501 gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag atttgcacgg
    16561 tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt acggctttgc
    16621 gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga tgtcaaccgg
    16681 tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga aaggtaatga
    16741 cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag aagagaaacc
    16801 gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc gactgatctt
    16861 caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg atcgttaccg
    16921 tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga aactgccggt
    16981 ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag cgtggatcct
    17041 cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg atatgcgcca
    17101 gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc gcctgccagc
    17161 gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct aagtagccac
    17221 atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac acgctgcgat
    17281 tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga cgctggcgcg
    17341 tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc atccggcacg
    17401 aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc
    17461 caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg
    17521 gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct gacgatatgg
    17581 tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc tcctccgaca
    17641 cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt gtgcatacgc
    17701 actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca accggcacca
    17761 cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa
    17821 tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt gaaaaacagg
    17881 gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg tttgcatggg
    17941 gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc gcttatatgg
    18001 ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg ctgctgcata
    18061 aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga ctgtataaaa
    18121 ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat ggattttcag
    18181 cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc aagggacaga
    18241 agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg caccgcaaga
    18301 gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt tgcagggtga
    18361 acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc cggtgtatgg
    18421 cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc gtgaaggtgg
    18481 cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat
    18541 cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta atgcccgtct
    18601 gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata ttgaaaccac
    18661 ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca tcgtttatat
    18721 gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat acgtcgccag
    18781 ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc ccgatgtgat
    18841 catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg ccgagcgtta
    18901 ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg agcacggctt
    18961 taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg gtatcgaggc
    19021 gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg ctcaggagct
    19081 attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa ttgaccgccg
    19141 tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac
    19201 gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa cggtgaacgg
    19261 cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg
    19321 aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc acgccagccc
    19381 tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg tgctggacta
    19441 taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggttga
    19501 aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg cctggttctc
    19561 acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc gcgataaagc
    19621 caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga atgcctttta
    19681 tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat cgtcgatcac
    19741 catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc agggctacga
    19801 cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac attcggaaga
    19861 agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt gggcggaaac
    19921 gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc atttctgccg
    19981 ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct acgccgggct
    20041 gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga
    20101 ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct tccgcaacga
    20161 gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg aactggatgc
    20221 gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc gtaatgttcc
    20281 gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg gtcgcccctt
    20341 gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc cggagccgct
    20401 ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt
    20461 ggcggaggga atactccctt ttattgagga taattttgct acacttatga ccgggcaact
    20521 tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc
    20581 tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca ccactacctc
    20641 ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg
    20701 ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc
    20761 gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt
    20821 ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgataccatt
    20881 accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg
    20941 acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc
    21001 cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg gcagattgac
    21061 cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt
    21121 atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca gctcaacatc
    21181 gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga agtgggttta
    21241 gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa
    21301 cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc
    21361 cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc tcagcacgat
    21421 gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt tgcccgtcgt
    21481 agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa
    21541 gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa
    21601 ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca gctggggatg
    21661 gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag
    21721 ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca
    21781 ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg cgagcaggat
    21841 atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag cgcccgtcag
    21901 gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacg
    21961 cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct gccgctaccg
    22021 acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa
    22081 gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga aggtgataac
    22141 gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat
    22201 cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca actggagcag
    22261 gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat gtcgattatc
    22321 gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg
    22381 tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat ggtgatgttt
    22441 gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga tcgtatcggc
    22501 caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca gtcggtgctg
    22561 gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac cggacgcact
    22621 atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa
    22681 ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa agcacagctg
    22741 gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca
    22801 ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac
    22861 ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacg
    22921 ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc
    22981 acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac ctgggagcat
    23041 ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag cagcacgatt
    23101 tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact gatttatgtg
    23161 gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta
    23221 cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt tgaaaccttt
    23281 aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa cgccgtgcag
    23341 caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc tgcccgtgca
    23401 ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg
    23461 gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgagagc
    23521 aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga tgccctgcgt
    23581 ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac tacaatccac
    23641 cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg gtcaacaagc
    23701 cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc gtgatgacgc
    23761 gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg
    23821 gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg
    23881 agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cccgcagaag
    23941 gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt
    24001 acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg gcggataaca
    24061 cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc
    24121 tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa gcgagagcga
    24181 tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca
    24241 atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc agttcggtag
    24301 gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga tgcgacgcgg
    24361 gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc tgctttatca
    24421 gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc atctcaggcg
    24481 gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta cggtaggcac
    24541 gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag gcatcttcca
    24601 gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc tgataaccgc
    24661 cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga
    24721 tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc agtgaaccat
    24781 cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca aaacgaccaa
    24841 agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct gattttccca
    24901 cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt cggtccatca
    24961 actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg gttaaatgcc
    25021 ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc
    25081 acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg cctaacacta
    25141 cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt tttccccaat
    25201 actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac aatataaagg
    25261 tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat ttgcattatc
    25321 atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag gattaacact
    25381 agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac gttaccgatg
    25441 atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc caccatgatt
    25501 gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca gtgcatgttg
    25561 ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt acccgtgact
    25621 atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac tggcagcgtg
    25681 gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag
    25741 gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac
    25801 gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac caaagatacc
    25861 aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg
    25921 atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt tacctcctgt
    25981 ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa
    26041 caagttgctg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat cttttatagc
    26101 ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc
    26161 aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa catcgcgcca
    26221 aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat catgtgggag
    26281 aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga ctatctgcct
    26341 tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg ttggttattc
    26401 tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga ctacaccaag
    26461 gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag tactgacggc
    26521 tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc caccgtttca
    26581 accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc agagccgcag
    26641 ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat ttacggccag
    26701 gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt tcacctggaa
    26761 ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga agcgaagttt
    26821 ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa cacgaccaag
    26881 ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa aatggtcttt
    26941 gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag
    27001 tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc gtctctgctg
    27061 caatctgact actctggcct gttccgggac cggacttacg gcggtcttga ccgtattgcc
    27121 tccgctaacc aagtgacgac cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa
    27181 cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac
    27241 aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg cgacacctac
    27301 tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac acgtctggat
    27361 aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg tctggtacag
    27421 ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa gtactattcc
    27481 actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag caggccaatt
    27541 gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa caagcaagcc
    27601 gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt cggttacgag
    27661 cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa cgcaatcggc
    27721 tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca agagatgctg
    27781 cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc acgtaatccg
    27841 cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat
    27901 cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag tcgcagccgt
    27961 cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt cggtaaaact
    28021 gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc accaaatcat
    28081 ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat
    28141 ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca acatgacgct
    28201 ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt atcgtaacca
    28261 gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac
    28321 catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa acgacgccag
    28381 cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga cctctgatca
    28441 ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta acggcgctga
    28501 tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg gcggccagat
    28561 ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa gcaccgcgaa
    28621 gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc tgaaagttaa
    28681 cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc gccatattct
    28741 gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg agcaaattgc
    28801 tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt tctcgcagga
    28861 tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata ttttcgatcc
    28921 agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac cggttcactc
    28981 ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata aaaccgacgc
    29041 tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg aagaagcagc
    29101 aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca actaatggtt
    29161 aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc ggacttagtt
    29221 gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga tgccactctc
    29281 cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta ttcccccaac
    29341 tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa
    29401 tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga gacgctggcg
    29461 cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat
    29521 aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt tttcgaagag
    29581 cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg
    29641 gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc acttttgcac
    29701 gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc cgaaccgcgc
    29761 attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag
    29821 atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa actcaacggg
    29881 ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga cgccgtgctg
    29941 gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg
    30001 aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac cgcgcttgaa
    30061 ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa tctcgccatc
    30121 aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta gcccgtaaac
    30181 gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt tctgccatta
    30241 acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca ttgaccgagc
    30301 cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat ctggcggcac
    30361 gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag gatgcgatga
    30421 cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc
    30481 tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact gatgccattg
    30541 ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca ggaccgaaca
    30601 gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac
    30661 tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc
    30721 tggtccctca tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg agccgcatca
    30781 ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta
    30841 gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa aatatctctg
    30901 tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg caggagagtt
    30961 aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca
    31021 atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac gcaatctggg
    31081 gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca atggccgtga
    31141 aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga
    31201 gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc agggtcacta
    31261 cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat tccgactcgc
    31321 cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg acgttcatgg
    31381 ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg ggaaagatac
    31441 cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg ttctgcgcta
    31501 tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct
    31561 ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac cgctgctgga
    31621 agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc tgcaaatcga
    31681 cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg atctgcagac
    31741 cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct atcccttctt
    31801 tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc gggggctggg
    31861 aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc cgaacggtca
    31921 actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga aaccgtggtt
    31981 tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact gggcatcgct
    32041 ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct gctggggtgg
    32101 gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt cgaaccggca
    32161 taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa ggccggataa
    32221 gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt accgccgctc
    32281 cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga attcgctgaa
    32341 taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt ccacttctgc
    32401 gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat aaacgcgacc
    32461 gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt catccaccga
    32521 tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa tatttttgcg
    32581 tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca cgggtttatt
    32641 taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca tggcgttttc
    32701 catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg agatttcccg
    32761 ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg
    32821 atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg gactcgccgc
    32881 attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc actctttatt
    32941 aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta ccttcttctg
    33001 ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg gtgatgatct
    33061 cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg tcattctcaa
    33121 ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg gcacgttctc
    33181 gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt ttcagcgcac
    33241 cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga atgtagtgat
    33301 cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc accatctccg
    33361 tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc gctttcgccg
    33421 ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact ttcataccaa
    33481 atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt ttcaccccgc
    33541 tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg ataatcacgc
    33601 gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa gattgctcaa
    33661 ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc accgccaggg
    33721 tcagcgattt cacccactcc ggctccagca cattcgccat ctgcgccgca ccaaatacca
    33781 caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc tgtttatttg
    33841 gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg aggaaaccga
    33901 gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct atcgacatgc
    33961 caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata tcgctttcca
    34021 acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg cccatcgcca
    34081 tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat aacgccacgg
    34141 cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga cgcgtaacat
    34201 agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc gagagagcaa
    34261 atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc gccaccagcg
    34321 ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc atttgcgtca
    34381 ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag agcgccagcg
    34441 tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag aacagcccca
    34501 gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc gcacgcagct
    34561 tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc accaccccaa
    34621 tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc cacgggccaa
    34681 taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt accgcaatgg
    34741 gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta tgactatcca
    34801 tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg cccttcgagg
    34861 gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg
    34921 cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg atgcgcacca
    34981 atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg tttgccatgc
    35041 agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc gatccaaagt
    35101 ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat cagatcggcg
    35161 cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata gagttgataa
    35221 agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg tttattcgca
    35281 tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat tagcccgcgt
    35341 gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata
    35401 atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc ccgcggatcg
    35461 tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac gctacggaaa
    35521 tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg ataccgtaat
    35581 tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga cttaaggaca
    35641 taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta ggatagagcc
    35701 actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc atcactacca
    35761 gccagatccc catatccggg attagcccca aaaccgccaa cgccagcgtc atcatggcaa
    35821 agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc ggcggaatac
    35881 agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt ttgccaaccc
    35941 ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac attgggatca
    36001 cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca
    36061 gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt tcgccaaaca
    36121 gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac cagcggggag
    36181 attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc gcgctaccca
    36241 gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga tcggcgcagg
    36301 tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc tgacgggtac
    36361 tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg acaatgccga
    36421 tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc gccaccgata
    36481 tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact ttatcggaga
    36541 tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc
    36601 caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc acgccaatca
    36661 ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa atgcggcggt
    36721 gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt
    36781 tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc gtaacggaac
    36841 tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc cagccccaga
    36901 atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc
    36961 tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac ctgtttatca
    37021 gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt attgacgccc
    37081 agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct
    37141 tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg cgcatcagtt
    37201 ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc agttccgggt
    37261 tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac atgcgcatat
    37321 cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc ttactgaaat
    37381 cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg gcaatcgcca
    37441 gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca tcaccggcaa
    37501 tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc ggcactacgt
    37561 gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc ggatgctgtt
    37621 tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga tgcagaccac
    37681 aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc
    37741 catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc aactgaaaac
    37801 ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg
    37861 tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc accccatcag
    37921 caaggagcac cgttttcgct tcaatcacat caccatcggc ttctacaccg acgactttgc
    37981 catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg cccgcttctt
    38041 cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg
    38101 atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt tccgtcataa
    38161 acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg aaacctggaa
    38221 taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg gcacctgcgg
    38281 aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc agtgcggcaa
    38341 ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg gacataagag
    38401 ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac gcgctaaagc
    38461 tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat ccgcgtactg
    38521 gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg cgttagcgcc
    38581 aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag gtttcagcat
    38641 caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt ctgccaccgg
    38701 acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt
    38761 ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac
    38821 gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca
    38881 ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat cgaacgtgcc
    38941 gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc
    39001 gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg tgctggcatc
    39061 gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg
    39121 ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt cagccatgac
    39181 accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt
    39241 agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg cattgatttg
    39301 attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg tatcgctgaa
    39361 tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga
    39421 ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgc
    39481 tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc gttaaaacca
    39541 atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat ggctttcatc
    39601 gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg cagcggaatg
    39661 cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg
    39721 gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc caccagcaga
    39781 ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag atcaaagcct
    39841 gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac
    39901 tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc tttacgccct
    39961 ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc ctctgctgcc
    40021 tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct tattttggca
    40081 tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc ctgttcatca
    40141 ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat gaaggaaacg
    40201 tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga aataaaactt
    40261 taatcaccaa tattgaaagt cagatgctat ttttttaccc acaaaagaat ataacaaact
    40321 gattaataac caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa aatgtgatac
    40381 caatcacaga atacagctta ttgaataccc attatgagtt agccattaac gcgtccacga
    40441 tgttaataat aattatatta aatgttaaca aaaataaaac aaacgggaac gcaaaataaa
    40501 tattcgtttt cacagtggaa ttaactcatg aagaatgaaa agagaaaaac gggaatagaa
    40561 ccgaaggttt tctttccgcc attaataatc gtcggcatac tttgttggct tacagtcaga
    40621 gatctggatg cggcgaatgt cgttattaat gctgtattca gttacgtcac caatgtatgg
    40681 ggatgggcat ttgaatggta tatggtggtg atgcttttcg gttggttctg gctggtgttt
    40741 ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag aatttagcac cgccagttgg
    40801 atctttatga tgttcgcctc ctgtacgtct gctgccgtac tgttctgggg atcgattgag
    40861 atctactact acatctccac cccgccgttt ggcttagaac cgaactcgac aggagcgaaa
    40921 gagttggggc tggcttacag cttgttccac tggggacctc tgccgtgggc cacttacagc
    40981 ttcctttcag tcgccttcgc ttacttcttc tttgtccgca aaatggaagt gattcgcccc
    41041 agctcgacac tggtgccgct ggcaggtgaa aaacacgcca aagggttgtt cggtaccatc
    41101 gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct gggccttgcc
    41161 acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac cctgcaactg
    41221 gacgctatca tcattacctg ctggattatc ctcaacgcca tttgcgtcgc ttgcggtctg
    41281 caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct gatgctgggt
    41341 tgggtgttca tcgtcagcgg tgccagcttc atcatgaact acttcaccga ttcggtgggg
    41401 atgttgctga tgtatctgcc gcgcatgttg ttctataccg atcccatcgc taaaggcggc
    41461 ttcccgcagg gctggaccgt gttctactgg gcatggtggg tgatttacgc catccagatg
    41521 agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg cttcggcatg
    41581 gtgatggggc tgacagcatc cacctggatc ctgtggaccg tgctcggtag taacactctg
    41641 ctgttgatgg ataaaaacat catcaacatt ccaaatctga tcgaacagta cggtgtggcg
    41701 cgcgccatca tcgaaacctg ggccgctctg ccactcagca ccgccaccat gtggggcttc
    41761 ttcatcctct gctttattgc caccgttacg ctggttaacg cctgctctta taccctggcg
    41821 atgtctactt gccgcgaagt acgcgatggt gaagaaccac cgctgctggt gcgtatcggt
    41881 tggtcaattc tggttggcat tatcggtatt gttctgctgg cgctcggcgg cctgaaaccg
    41941 attcaaaccg ccattatcgc cggaggatgc ccgctgttct tcgtcaacat tatggtgacg
    42001 ctctccttta ttaaagacgc gaaacagaac tggaaagatt aattaacccc caaaatatca
    42061 agaggttgaa agatggattt taatttaaat gatgagcagg aactgtttgt cgccggtatc
    42121 cgcgaactga tggccagcga aaactgggag gcctattttg ccgagtgcga ccgtgacagc
    42181 gtctacccgg aacgttttgt caaagcactg gcggatatgg gtatcgacag tctgctgatc
    42241 cctgaagagc acggtggtct ggacgcgggg tttgttactc tcgccgccgt gtggatggag
    42301 ctgggacgtc tgggggcacc aacctatgta ctgtaccagc tgccgggcgg gttcaatacc
    42361 ttcctgcgcg aaggcacaca agagcagatc gacaagatta tggctttccg cggcaccggt
    42421 aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt gggtagcctg
    42481 aaaacgactt atacccgtag aaatggtaag atttatctta atggtagtaa gtgttttatt
    42541 accagtagcg cctacacccc gtacatcgtg gtgatggcgc gcgacggggc ttctccggac
    42601 aaacctgtct acaccgaatg gtttgttgat atgagcaaac cgggcatcaa agtgaccaaa
    42661 cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa tcacctttga cgatgtggaa
    42721 ctggacgaga aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt caaagaagag
    42781 ttcgaccatg aacgtttcct ggtagccctg accaactacg gtacggcgat gtgcgccttt
    42841 gaagatgctg cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat tggtcgtttc
    42901 cagttgattc aggaaaaatt cgcccacatg gcgatcaaat taaactccat gaaaaacatg
    42961 ctgtatgaag cagcgtggaa agcagacaac ggcaccatca cctctggcga tgcagcgatg
    43021 tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat gcaggtgctg
    43081 ggcggtgtcg ggattgcggg caaccaccgc atcagccgct tctggcgtga cctgcgtgta
    43141 gaccgcgttt ccggaggatc tgacgaaatg cagatcctga cgctgggtcg tgcggtgctg
    43201 aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc tgacgcgtct tatcaggcct
    43261 acgggagcac gaatgtaagc cggataaggc gtttacgccg catccggcag tcatgcgcta
    43321 cataaatttt tcaggagcta ataatggatc atctacccat gccgaaattc ggaccgctgg
    43381 ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc ggacaaatgt
    43441 tcgcagaatg gggcgcggaa gttatttgga tcgagaacgt cgcctgggcc gacaccattc
    43501 gcgttcaacc gaactacccg cagctctccc gccgcaattt gcacgcgctg tcgttaaata
    43561 ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc gatatcttca
    43621 tcgaagccag taaaggtccg gcctttgccc gtcgtggcat taccgatgaa gtactgtggc
    43681 agcacaaccc gaaactggtt atcgctcacc tgtccggttt tggtcagtac ggcaccgagg
    43741 agtacaccaa tcttccggcc tataacacca tcgcccaggc cttcagtggt tacctgattc
    43801 agaacggtga tgttgaccag ccaatgcctg ccttcccgta taccgccgat tacttttctg
    43861 gcctgaccgc caccacggcg gcgctggcag cactgcataa agtgcgtgaa accggtaaag
    43921 gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg tatgggccag tacttcatga
    43981 tggattactt caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa gatccctact
    44041 acgccggttg cggtctgtat aaatgtgccg acggctacat cgtgatggaa ctggtgggca
    44101 ttacccaaat tgaagagtgc tttaaagata ttggcctcgc acatctactc ggtacgccgg
    44161 aaatcccgga aggcactcag cttatccacc gtatcgaatg cccttacggc ccactggtag
    44221 aagaaaaact cgatgcctgg ctggcggcac ataccatcgc agaagttaaa gaacgctttg
    44281 ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa agcaatccac
    44341 agtatgttgc ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc acctgcaaag
    44401 ggccgaacat catgccgaaa ttcaaaaata accccggaca aatctggcgc ggaatgccct
    44461 cacatggcat ggacacggct gccattttga aaaatatcgg ctacagcgaa aacgacattc
    44521 aggagttggt cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc gtcagtcggc
    44581 ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa tggatagagg tgcaatggat
    44641 atcattggcg gacaacatct acgtcaaatg tgggacgatc tggcggacgt ttacggtcat
    44701 aaaacggcgc tgatttgtga atccagcggc ggagtcgtta accggtatag ttatcttgag
    44761 ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat tcgcaaaggc
    44821 aacaaggttg cactacatct cgacaactgc ccggaattta tcttttgctg gttcgggctg
    44881 gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga ggaaagcgag
    44941 tggatcttgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt ttatcctatg
    45001 tatcaacaga ttcagcagga agatgccact caattgcggc acatttgcct gacagatgtg
    45061 gcacttcccg ctgatgatgg agtgagttca tttactcaac tgaaaaatca acaacctgcc
    45121 accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct cttcacctcc
    45181 ggcaccacct cccgaccgaa aggtgtggtg attacccatt acaacctgcg cttcgctgga
    45241 tattactccg cctggcagtg tgcactgcgt gacgatgacg tctacatgac ggtaatgcct
    45301 gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc cggggccacc
    45361 tttgtgctgg tcgagaaata cagcgcccgc gccttctggg gacaggcgca gaagtaccgc
    45421 gccaccatta ccgaatgtat tccgatgatg atccgtacgt tgatggtgca gccgccttca
    45481 gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt gtcggagcag
    45541 gaaaaagacg cattttgtga acgcttcggc gttcgcttgc tgacgtctta tgggatgacg
    45601 gaaaccattg tgggcattat cggcgatcgc cctagcgata aacgacgctg gccgtcgatt
    45661 ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg cccgctcccg
    45721 gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat cttcaaagag
    45781 tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgacggctg gctacatacc
    45841 ggcgataccg gataccgcga cgaagaaggc tttttttatt tcgtcgatcg ccgctgcaat
    45901 atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat catcgccacc
    45961 catccgaaaa ttcaggacat cgtggttgtg ggtattaaag attcgattcg cgatgaagcc
    46021 atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga attttttcgc
    46081 ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat cagaaaagat
    46141 ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat gaaacgtcag
    46201 ggaactaccc tgccagctaa taatcacgcc ctaaaacaat atgcattttt tgcagggatg
    46261 ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga gtgaatcatt acatctgacc
    46321 cgcaatggat caattctgga aattaccctt gatcgcccaa aagcgaatgc tattgatgca
    46381 aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc gcaattacgt
    46441 gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga tttaaaagcg
    46501 gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggttttgc gggattaacc
    46561 gaaattttca atctcgacaa accagttatc gcagctgtga acggctacgc ctttggcggc
    46621 ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc cagcttcgcc
    46681 ctgccggaag ccaaactggg catcgttcct gacagcggcg gtgtgctgcg tctgccgaag
    46741 atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat gggcgcagaa
    46801 gaggcgctgc gttgggggat agtcaaccgc gtggttagcc aggcggaact gatggataac
    46861 gcccgcgaac tggctcagca gctggttaac agcgccccgc tggcgattgc ggcgctgaaa
    46921 gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta tattcgcagc
    46981 ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga agggccgctg
    47041 gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg agctattacg
    47101 cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc agtgccgtct
    47161 tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc tcactgcgtg
    47221 gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc tgcattatgc
    47281 atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg catggagcga
    47341 tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc gtaattatgg
    47401 atggcgcggt cattggcgaa gagagcattg ttgccgcaat gagctttgtc aaagcaggct
    47461 ttagtggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta cgcagtgtta
    47521 gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat cttgtcgggc
    47581 gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa aatcgccccc
    47641 gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg cgacctggtt
    47701 gctggataag atgctacaac atcgtatctg gcaagcagga cgcccggtat caacgagata
    47761 aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc ggttaagttc
    47821 cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg ccgactaatt tttcccgact
    47881 gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa ccagtcgctg
    47941 acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc agcttatttc
    48001 tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt caatatcgaa
    48061 atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc attcggcgat
    48121 taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag tttcacaaat
    48181 taaaagtcta gctatgactc gttttgaagc aattaaacaa ggccatatta aaattgtgga
    48241 tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg cttatgtcat
    48301 caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaaacgg tcctcatcag
    48361 agaaccgtat tagttattcg atcaaattag cggatgaaaa atatccgcca cgacacgcta
    48421 ttatttgatc tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg catcggcatt
    48481 cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca ctttatattg
    48541 cagcgcactg cgacggatca cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt
    48601 gatgatgtag gtatattcgc cattcttgat acggtcctga atgtgcggac ggccttcatg
    48661 caccttgttt accagacgcg gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg
    48721 ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca ccacgcgttc
    48781 tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct
    48841 gcccagctgc gctttggcga acgcttcagc gaaggtgcgg cccacgccca tgacttcccc
    48901 ggtagagcgc atttctggcc ctaacagcgg gtcaacgcct gggaatttat tgaacggcag
    48961 caccacttct ttcaccgagt agtacggcgg aataacttct ttggttacgc cctgctcagc
    49021 cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc cggtggcttt
    49081 ggagacgaac ggaacagtac gcgccgcacg cgggtttact tcaatcaggt aaacttcgtt
    49141 gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga aggccagttt
    49201 ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtacg ctggcagcga
    49261 acatgcggag tcaccggagt gcacacccgc ctgctcgatg tgctccatga tgccgccaat
    49321 cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctactgcgt catcaaggaa
    49381 atgatccagc aacactggcg catcgttaga cacgctaacc gccgtctgga agtagcgacg
    49441 caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt aagacggacg
    49501 caccaccagc gggtagccaa tctcttttgc cttctcaact gccatttcaa tagcagtaac
    49561 ggtggcgttc gccggttgtt tcagtttcag acggtcaacc gcatgctgga agcgttcacg
    49621 gtcttccgca cggtcgatag catccgggct ggtgccgata accggtacgc cagcggcttc
    49681 cagtgcacgc gccagtttca gcggggtctg accgccgtac tgaacgataa cgcctttcgg
    49741 cttctcaata cgcacgattt ccagcacatc ttccagagtt accggctcga agtagaggcg
    49801 atcggaagtg tcgtagtcgg tagagacggt ttccgggtta cagttaacca taatggtttc
    49861 gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga attcgatacc
    49921 ctgaccgata cggtttggac cgccgccaag caccatgatt ttttcacggt cggtagacgg
    49981 attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg tggcaaactc
    50041 tgccgcacag gtatccacgc gcttgtagac cgggtgcagg tcatattggt cacgcagctt
    50101 acggatttcc gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg caaagccttt
    50161 gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt ccgccacttt
    50221 ctcttccaga cgcaccagtt cttcaatctg taccaggaac cagcggtcaa tgttagtcag
    50281 gttgaagacg ccgtccacag acaggcccgc gcggaaagca tcggcgatgt accagatacg
    50341 ctctgcgcct gcgtctttca gttcgcgacg gattttggtt aacgcttccg ggtcatccag
    50401 gctcactttc gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg ctttttgcag
    50461 ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca tctgagtggt
    50521 cagacggtcg ttagcaccgg cgaatttttc gaagttgaag cgaggaattt tagtaaccac
    50581 atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt cgttcatcag
    50641 ttcgtctagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg ggaaaccggt
    50701 cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga taacaatcag
    50761 gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt caacgccgat
    50821 ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt cggtcagcgt
    50881 ttgggctggc gcgacagtga tggagtcacc ggtgtggatg cccatcgcat caaagttttc
    50941 gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca tctcgtactc
    51001 tttccagccg atcagcgact catcaatcag caactctttg gtcggagaga gatccagacc
    51061 gcgggcgcaa atttcttcaa actcttcacg gttataagcg ataccgccgc cgctaccgcc
    51121 catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa cagccagcgc
    51181 ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt tcttcatcgc
    51241 tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcagtgg caccaatcat
    51301 ggtgacaccg aactcttcca acacgccctg acgttccagc tccagcgcgc agttcagcgc
    51361 cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg
    51421 taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat
    51481 gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt
    51541 acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa tcgggcccgc
    51601 acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt tactcctgat
    51661 tacttagcgg ttttacggta ctgctcaatt aacttgataa agtggtcgaa caacggcgcg
    51721 gcgtcgtgtg gaccagggct ggcttcaggg tgaccctgga agctgaacgc cggcttatcg
    51781 atgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt
    51841 gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat catcaccacg
    51901 tttttctcca catctttaac cggatggttg ccgccgtggt gaccaaattt cattttgaca
    51961 gtcttcgcac cgctcgccag cgccagcagc tgatgaccga gacagatgcc gaataccgga
    52021 atatcggttt cgaggaactt ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg
    52081 ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc ttccgcagaa
    52141 gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatccg caggatgttg
    52201 cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc ttttttcgct
    52261 tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc ttctgcggtg
    52321 gtcacttctt ttgccagatc catgccgttc agacctggga acgcgcgggc tttttctaac
    52381 gccagcgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc gcctttctcg
    52441 cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat gttatggcgc
    52501 ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag cggcaggtcg
    52561 cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc attagtgccg
    52621 acattgccaa tatggggata agtaagagta acgatttgac gagaatagga aggatcagtg
    52681 aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac cgccgaacct
    52741 gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac
    52801 ttaatcaaaa caccctccag agaatactca ctcactttat ttgcatatta attcacagtg
    52861 atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg cggcattctg
    52921 gagatatggg cgctaaaagt caacttaatg gcaggatttt ttatattatt ttgtggctca
    52981 tgcatgtttt acaggcatta agcgacaaaa acatcatcta acctgttctg gaaaacgctt
    53041 gcgcagcaag aagattgcat gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa
    53101 aatttacatt aaaacaacaa aaatagaaga cgaaacatgt aatttttggc agttatagaa
    53161 aatttaaaag cataacacca
//
LOCUS       DAZPDE010000008        49829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPDE010000008 DAZPDE010000000
VERSION     DAZPDE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.008"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHU0J_000403"
     CDS             <1..1224
                     /locus_tag="ACHU0J_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1215250.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHU0J_000404"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHU0J_000404"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1215251.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHU0J_000405"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHU0J_000405"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR1215252.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHU0J_000406"
     CDS             2714..3427
                     /locus_tag="ACHU0J_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1215253.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHU0J_000407"
     CDS             complement(3399..4076)
                     /locus_tag="ACHU0J_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1215254.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHU0J_000408"
     CDS             complement(4070..4747)
                     /locus_tag="ACHU0J_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1215255.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHU0J_000409"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHU0J_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHU0J_000410"
     CDS             complement(5443..6021)
                     /locus_tag="ACHU0J_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1215256.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHU0J_000411"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHU0J_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHU0J_000412"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHU0J_000412"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1215257.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHU0J_000413"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHU0J_000413"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1215258.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHU0J_000414"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHU0J_000414"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1215259.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHU0J_000415"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHU0J_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1215260.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHU0J_000416"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHU0J_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1215261.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHU0J_000417"
     CDS             12291..13031
                     /locus_tag="ACHU0J_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1215262.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHU0J_000418"
     CDS             13198..14148
                     /locus_tag="ACHU0J_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1215263.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHU0J_000419"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHU0J_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHU0J_000420"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHU0J_000420"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1215264.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHU0J_000421"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHU0J_000421"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1215265.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHU0J_000422"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHU0J_000422"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1215266.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPR
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHU0J_000423"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHU0J_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1215267.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHU0J_000424"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHU0J_000424"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1215268.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHU0J_000425"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHU0J_000425"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1215269.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHU0J_000426"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHU0J_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1215270.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHU0J_000427"
     CDS             23768..23827
                     /locus_tag="ACHU0J_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1215271.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHU0J_000428"
     CDS             complement(23928..24530)
                     /locus_tag="ACHU0J_000428"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1215272.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHU0J_000429"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHU0J_000429"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHU0J_000430"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHU0J_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1215273.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHU0J_000431"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHU0J_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1215274.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHU0J_000432"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHU0J_000432"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1215275.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHU0J_000433"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHU0J_000433"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1215276.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHU0J_000434"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHU0J_000434"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1215277.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACHU0J_000435"
     CDS             complement(29676..29759)
                     /locus_tag="ACHU0J_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215278.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHU0J_000436"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHU0J_000436"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1215279.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHU0J_000437"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHU0J_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1215280.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHU0J_000438"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHU0J_000438"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1215281.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="ACHU0J_000439"
     CDS             34816..35559
                     /locus_tag="ACHU0J_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1215282.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHU0J_000440"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHU0J_000440"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1215283.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHU0J_000441"
                     /pseudo
     CDS             37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHU0J_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37568..38227
                     /locus_tag="ACHU0J_000442"
     CDS             37568..38227
                     /locus_tag="ACHU0J_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1215284.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHU0J_000443"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHU0J_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1215285.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHU0J_000444"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHU0J_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1215286.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="ACHU0J_000445"
     CDS             39893..40300
                     /locus_tag="ACHU0J_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1215287.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHU0J_000446"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHU0J_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1215288.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="ACHU0J_000447"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="ACHU0J_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1215289.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHU0J_000448"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHU0J_000448"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1215290.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="ACHU0J_000449"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="ACHU0J_000449"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1215291.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="ACHU0J_000450"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="ACHU0J_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1215292.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="ACHU0J_000451"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="ACHU0J_000451"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1215293.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHU0J_000452"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHU0J_000452"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1215294.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="ACHU0J_000453"
     CDS             47705..47776
                     /locus_tag="ACHU0J_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215295.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHU0J_000454"
     CDS             complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHU0J_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1215296.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHU0J_000455"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHU0J_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR1215297.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12050 a        12450 c        13406 g        11923 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggcccgtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgcagtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtc
//
LOCUS       DAZPDE010000009        49532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPDE010000009 DAZPDE010000000
VERSION     DAZPDE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.009"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHU0J_000456"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHU0J_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1215298.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHU0J_000457"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHU0J_000457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1215299.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHU0J_000458"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHU0J_000458"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1215300.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHU0J_000459"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHU0J_000459"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1215301.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHU0J_000460"
     CDS             5718..6659
                     /locus_tag="ACHU0J_000460"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1215302.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHU0J_000461"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHU0J_000461"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1215303.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHU0J_000462"
     CDS             7679..8929
                     /locus_tag="ACHU0J_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1215304.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHU0J_000463"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHU0J_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHU0J_000464"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHU0J_000464"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1215305.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHU0J_000465"
     CDS             10866..12116
                     /locus_tag="ACHU0J_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1215306.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHU0J_000466"
     CDS             complement(12172..13260)
                     /locus_tag="ACHU0J_000466"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1215307.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHU0J_000467"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHU0J_000467"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1215308.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHU0J_000468"
     CDS             complement(14194..15243)
                     /locus_tag="ACHU0J_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1215309.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHU0J_000469"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHU0J_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1215310.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHU0J_000470"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHU0J_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1215311.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHU0J_000471"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHU0J_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHU0J_000472"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHU0J_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHU0J_000473"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHU0J_000473"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1215312.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHU0J_000474"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHU0J_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1215313.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHU0J_000475"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHU0J_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1215314.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHU0J_000476"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHU0J_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1215315.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHU0J_000477"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHU0J_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1215316.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHU0J_000478"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHU0J_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1215317.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHU0J_000479"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHU0J_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHU0J_000480"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHU0J_000480"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR1215318.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="ACHU0J_000481"
     CDS             complement(30809..31048)
                     /locus_tag="ACHU0J_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1215319.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHU0J_000482"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHU0J_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1215320.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHU0J_000483"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHU0J_000483"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1215321.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="ACHU0J_000484"
     CDS             complement(32934..33629)
                     /locus_tag="ACHU0J_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1215322.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="ACHU0J_000485"
     CDS             complement(33626..34024)
                     /locus_tag="ACHU0J_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1215323.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="ACHU0J_000486"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="ACHU0J_000486"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1215324.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHU0J_000487"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHU0J_000487"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1215325.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHU0J_000488"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHU0J_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37392..38138
                     /locus_tag="ACHU0J_000489"
                     /pseudo
     CDS             37392..38138
                     /locus_tag="ACHU0J_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="ACHU0J_000490"
     CDS             complement(38255..38833)
                     /locus_tag="ACHU0J_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1215326.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="ACHU0J_000491"
     CDS             39003..39590
                     /locus_tag="ACHU0J_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1215327.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHU0J_000492"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHU0J_000492"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1215328.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHU0J_000493"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHU0J_000493"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1215329.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="ACHU0J_000494"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="ACHU0J_000494"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1215330.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="ACHU0J_000495"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="ACHU0J_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1215331.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="ACHU0J_000496"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="ACHU0J_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1215332.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="ACHU0J_000497"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="ACHU0J_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1215333.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="ACHU0J_000498"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="ACHU0J_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1215334.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="ACHU0J_000499"
     CDS             complement(48911..49018)
                     /locus_tag="ACHU0J_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1215335.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49532)
                     /locus_tag="ACHU0J_000500"
                     /pseudo
     CDS             complement(49078..49532)
                     /locus_tag="ACHU0J_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12203 c        13311 g        12063 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttattg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgctgcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc cg
//
LOCUS       DAZPDE010000010        48467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPDE010000010 DAZPDE010000000
VERSION     DAZPDE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.010"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            135..1352
                     /gene="alaA"
                     /locus_tag="ACHU0J_000501"
     CDS             135..1352
                     /gene="alaA"
                     /locus_tag="ACHU0J_000501"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1215336.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1436..2035
                     /gene="yfbR"
                     /locus_tag="ACHU0J_000502"
     CDS             1436..2035
                     /gene="yfbR"
                     /locus_tag="ACHU0J_000502"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1215337.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2094..3926)
                     /locus_tag="ACHU0J_000503"
     CDS             complement(2094..3926)
                     /locus_tag="ACHU0J_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1215338.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4013..4663)
                     /gene="hxpA"
                     /locus_tag="ACHU0J_000504"
     CDS             complement(4013..4663)
                     /gene="hxpA"
                     /locus_tag="ACHU0J_000504"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR1215339.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4674..5168)
                     /locus_tag="ACHU0J_000505"
     CDS             complement(4674..5168)
                     /locus_tag="ACHU0J_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1215340.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5251..5706)
                     /gene="yfbV"
                     /locus_tag="ACHU0J_000506"
     CDS             complement(5251..5706)
                     /gene="yfbV"
                     /locus_tag="ACHU0J_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1215341.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6044..7246
                     /gene="ackA"
                     /locus_tag="ACHU0J_000507"
     CDS             6044..7246
                     /gene="ackA"
                     /locus_tag="ACHU0J_000507"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1215342.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7321..9465
                     /gene="pta"
                     /locus_tag="ACHU0J_000508"
     CDS             7321..9465
                     /gene="pta"
                     /locus_tag="ACHU0J_000508"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1215343.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9655..11175
                     /gene="yfcC"
                     /locus_tag="ACHU0J_000509"
     CDS             9655..11175
                     /gene="yfcC"
                     /locus_tag="ACHU0J_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1215344.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11208..11750)
                     /gene="yfcD"
                     /locus_tag="ACHU0J_000510"
     CDS             complement(11208..11750)
                     /gene="yfcD"
                     /locus_tag="ACHU0J_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1215345.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11808..12359)
                     /gene="yfcE"
                     /locus_tag="ACHU0J_000511"
     CDS             complement(11808..12359)
                     /gene="yfcE"
                     /locus_tag="ACHU0J_000511"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1215346.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12415..13059)
                     /gene="yfcF"
                     /locus_tag="ACHU0J_000512"
     CDS             complement(12415..13059)
                     /gene="yfcF"
                     /locus_tag="ACHU0J_000512"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1215347.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13195..13842
                     /gene="yfcG"
                     /locus_tag="ACHU0J_000513"
     CDS             13195..13842
                     /gene="yfcG"
                     /locus_tag="ACHU0J_000513"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1215348.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13899..14261
                     /gene="folX"
                     /locus_tag="ACHU0J_000514"
     CDS             13899..14261
                     /gene="folX"
                     /locus_tag="ACHU0J_000514"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1215349.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14282..15175
                     /locus_tag="ACHU0J_000515"
     CDS             14282..15175
                     /locus_tag="ACHU0J_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1215350.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15223..16113)
                     /gene="rpnB"
                     /locus_tag="ACHU0J_000516"
     CDS             complement(15223..16113)
                     /gene="rpnB"
                     /locus_tag="ACHU0J_000516"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1215351.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16310..17083)
                     /gene="hisP"
                     /locus_tag="ACHU0J_000517"
     CDS             complement(16310..17083)
                     /gene="hisP"
                     /locus_tag="ACHU0J_000517"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1215352.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17091..17807)
                     /locus_tag="ACHU0J_000518"
     CDS             complement(17091..17807)
                     /locus_tag="ACHU0J_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1215353.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17804..18490)
                     /gene="hisQ"
                     /locus_tag="ACHU0J_000519"
     CDS             complement(17804..18490)
                     /gene="hisQ"
                     /locus_tag="ACHU0J_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1215354.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18580..19362)
                     /gene="hisJ"
                     /locus_tag="ACHU0J_000520"
     CDS             complement(18580..19362)
                     /gene="hisJ"
                     /locus_tag="ACHU0J_000520"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1215355.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19583..20365)
                     /gene="argT"
                     /locus_tag="ACHU0J_000521"
     CDS             complement(19583..20365)
                     /gene="argT"
                     /locus_tag="ACHU0J_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1215356.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20631..21200)
                     /gene="ubiX"
                     /locus_tag="ACHU0J_000522"
     CDS             complement(20631..21200)
                     /gene="ubiX"
                     /locus_tag="ACHU0J_000522"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1215357.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21295..22812)
                     /gene="purF"
                     /locus_tag="ACHU0J_000523"
     CDS             complement(21295..22812)
                     /gene="purF"
                     /locus_tag="ACHU0J_000523"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1215358.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22849..23337)
                     /gene="cvpA"
                     /locus_tag="ACHU0J_000524"
     CDS             complement(22849..23337)
                     /gene="cvpA"
                     /locus_tag="ACHU0J_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1215359.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23596..24258)
                     /gene="dedD"
                     /locus_tag="ACHU0J_000525"
     CDS             complement(23596..24258)
                     /gene="dedD"
                     /locus_tag="ACHU0J_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1215360.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24248..25516)
                     /gene="folC"
                     /locus_tag="ACHU0J_000526"
     CDS             complement(24248..25516)
                     /gene="folC"
                     /locus_tag="ACHU0J_000526"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1215361.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25586..26500)
                     /gene="accD"
                     /locus_tag="ACHU0J_000527"
     CDS             complement(25586..26500)
                     /gene="accD"
                     /locus_tag="ACHU0J_000527"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1215362.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26656..27315)
                     /locus_tag="ACHU0J_000528"
     CDS             complement(26656..27315)
                     /locus_tag="ACHU0J_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1215363.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27398..28210)
                     /gene="truA"
                     /locus_tag="ACHU0J_000529"
     CDS             complement(27398..28210)
                     /gene="truA"
                     /locus_tag="ACHU0J_000529"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1215364.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28210..29223)
                     /locus_tag="ACHU0J_000530"
     CDS             complement(28210..29223)
                     /locus_tag="ACHU0J_000530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1215365.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29289..30425)
                     /gene="pdxB"
                     /locus_tag="ACHU0J_000531"
     CDS             complement(29289..30425)
                     /gene="pdxB"
                     /locus_tag="ACHU0J_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1215366.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30524..31519
                     /gene="flk"
                     /locus_tag="ACHU0J_000532"
     CDS             30524..31519
                     /gene="flk"
                     /locus_tag="ACHU0J_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1215367.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31516..32694)
                     /locus_tag="ACHU0J_000533"
     CDS             complement(31516..32694)
                     /locus_tag="ACHU0J_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1215368.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32949..34190)
                     /gene="fabB"
                     /locus_tag="ACHU0J_000534"
     CDS             complement(32949..34190)
                     /gene="fabB"
                     /locus_tag="ACHU0J_000534"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1215369.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34349..36355
                     /gene="mnmC"
                     /locus_tag="ACHU0J_000535"
     CDS             34349..36355
                     /gene="mnmC"
                     /locus_tag="ACHU0J_000535"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1215370.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36476..36754)
                     /locus_tag="ACHU0J_000536"
     CDS             complement(36476..36754)
                     /locus_tag="ACHU0J_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1215371.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(36788..37336)
                     /gene="epmC"
                     /locus_tag="ACHU0J_000537"
     CDS             complement(36788..37336)
                     /gene="epmC"
                     /locus_tag="ACHU0J_000537"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1215372.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37336..38145)
                     /locus_tag="ACHU0J_000538"
     CDS             complement(37336..38145)
                     /locus_tag="ACHU0J_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR1215373.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38145..38969)
                     /gene="mepA"
                     /locus_tag="ACHU0J_000539"
     CDS             complement(38145..38969)
                     /gene="mepA"
                     /locus_tag="ACHU0J_000539"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1215374.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38973..40058)
                     /gene="aroC"
                     /locus_tag="ACHU0J_000540"
     CDS             complement(38973..40058)
                     /gene="aroC"
                     /locus_tag="ACHU0J_000540"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1215375.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40093..41025)
                     /gene="prmB"
                     /locus_tag="ACHU0J_000541"
     CDS             complement(40093..41025)
                     /gene="prmB"
                     /locus_tag="ACHU0J_000541"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1215376.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41191..41742
                     /gene="smrB"
                     /locus_tag="ACHU0J_000542"
     CDS             41191..41742
                     /gene="smrB"
                     /locus_tag="ACHU0J_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1215377.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41846..42736)
                     /locus_tag="ACHU0J_000543"
     CDS             complement(41846..42736)
                     /locus_tag="ACHU0J_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1215378.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(42723..43247)
                     /locus_tag="ACHU0J_000544"
     CDS             complement(42723..43247)
                     /locus_tag="ACHU0J_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1215379.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43244..43714)
                     /locus_tag="ACHU0J_000545"
     CDS             complement(43244..43714)
                     /locus_tag="ACHU0J_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1215380.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(43711..44259)
                     /locus_tag="ACHU0J_000546"
     CDS             complement(43711..44259)
                     /locus_tag="ACHU0J_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1215381.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44264..44986)
                     /locus_tag="ACHU0J_000547"
                     /pseudo
     CDS             complement(44264..44986)
                     /locus_tag="ACHU0J_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45006..47646)
                     /gene="yfcU"
                     /locus_tag="ACHU0J_000548"
                     /pseudo
     CDS             complement(45006..47646)
                     /gene="yfcU"
                     /locus_tag="ACHU0J_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47728..48291)
                     /locus_tag="ACHU0J_000549"
     CDS             complement(47728..48291)
                     /locus_tag="ACHU0J_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1215382.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12012 a        13032 c        11972 g        11451 t
ORIGIN      
        1 cagcacaatc gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt
       61 cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa
      121 aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg
      181 acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac
      241 tgaaactgaa catcggcaac ccagccccat tcggttttga cgcgccagat gaaatcctcg
      301 ttgacgtgat acgcaacctg cctaccgctc aagggtattg cgattccaaa gggctttact
      361 ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg
      421 aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc
      481 tgaacagcgg ggacgaaatg ttggttcctg caccagatta cccactctgg accgcggcgg
      541 tttcgctttc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc
      601 cggacctcga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatt gttattatca
      661 acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga
      721 ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctctacg
      781 acgatgctga gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta
      841 acggactgtc gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg
      901 ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc
      961 tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca
     1021 tcagtgaatt tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac
     1081 tgatcaacga tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc
     1141 cgaaaatcga cgccaaacgc tttaacattc atgacgatca gaaaatggtg ctggatttcc
     1201 tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc
     1261 acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg
     1321 cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc
     1381 cggcagtgtt atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa
     1441 acagagccat ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctaat
     1501 gcgcaacgtg cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca
     1561 tgcgctggca gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc
     1621 tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt
     1681 gaaatacttc aatgcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca
     1741 gaaactggtc gatatggttc cggaagaact gcgggatatc tttgcgccgt taattgacga
     1801 gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata
     1861 tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg
     1921 actggaagcg acgcttgaag cgcgtcgcag ccaggagatg gactacttca tggaagtatt
     1981 tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc
     2041 cggagtctgc gtcgcatcag tcaataagcg ccggatgcga catcaggctc ttgtcaaaac
     2101 ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc
     2161 cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag ggtgttaaca
     2221 ggtgaagaca ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcaaat
     2281 ggataaggcg acactcccat cgttttggca gcagccagcg caatcggagc catcaacacc
     2341 gccgtcgcgg tattagagat aaacagccca ataaccgccg acaagacaaa caaacacccc
     2401 agcatcatat gtggcccata accgccgcca atatccatca gccctttcac cgccagcgcg
     2461 acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg
     2521 ctcggccagt gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc
     2581 agcaggcagg cgatgatagc ggcaacagga ttaggaattt catttgtcag cattaacgcc
     2641 accattagca ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca
     2701 ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg
     2761 atcagtttcc agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc
     2821 agcgaacctt ccagcgccac gccattgcgc ttgagcccca ccacattcag tccgtagcgg
     2881 gtacgaaaac caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa
     2941 atctctgcca tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc
     3001 ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc agacatatca
     3061 ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga
     3121 cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg
     3181 agatcatcca gccgttgacc tatcattggc gatccggggc gaatagccag acgtcgcgca
     3241 cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg
     3301 cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata
     3361 cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag
     3421 ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc
     3481 atgccgctaa tcagcccggc aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc
     3541 atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgct ggttgaactc
     3601 ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg
     3661 ttgcccgcca ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa
     3721 ccatcgccaa taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccggaa
     3781 aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg
     3841 accgcatcca tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa
     3901 agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga
     3961 ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa
     4021 taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca
     4081 aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatggcaac
     4141 ccgccgccag gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg
     4201 gcgcaagccc cagcagctgc gcacctaaca gatacgcatc aggttctggt tttccgcgct
     4261 tcactcgctc agcggttaca aacacctctg gtgcgggaag ccccgctatt ttatggcgcg
     4321 ctcgcgctac cggcatggag ccagaagtca caatggccca cggaatacct gctttattca
     4381 aatgattgag taaggcgatt gccccaggaa gcgcggtaat accttcggtt tccgtggcct
     4441 cgatttgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa
     4501 aatggcgcaa agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg
     4561 ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca
     4621 ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg
     4681 ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac
     4741 acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa accatgagtc
     4801 ccagcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg
     4861 taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat
     4921 tgctggtcct gcaaattaga ccaagaaaca tacaacgcat gatacatatc cataatgtcg
     4981 atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc
     5041 tgtaatccgt aaccacgctc aataattgtt tgcaggcgac ggtaacgttc agcatttgcc
     5101 ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg cgcgttggtc
     5161 atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag gctgagtatg
     5221 ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag
     5281 ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac
     5341 gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa accagttaag
     5401 gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc cctgcatggg
     5461 taaacttaag gcgaacagtg cagtggcaac tgccggccca agcagaccgc ccagggcaat
     5521 ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat
     5581 cttgataacg cgattttcga caaagaccgg agcaaggcgt ttttccagtg gccacgtctt
     5641 tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt
     5701 tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact
     5761 cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca
     5821 gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat
     5881 tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa
     5941 atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc
     6001 tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt
     6061 actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg
     6121 tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg
     6181 gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc
     6241 gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac
     6301 tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga
     6361 tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc
     6421 tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt
     6481 tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct
     6541 gccgtacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca
     6601 cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat
     6661 catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt
     6721 tgacacctct atgggtctga ccccgctgga aggtctggtc atgggtaccc gttctggtga
     6781 tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat
     6841 caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg
     6901 ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta
     6961 ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga
     7021 cgctgttgca ttcactggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct
     7081 gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt
     7141 cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa
     7201 cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag
     7261 ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc
     7321 gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc
     7381 cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc
     7441 gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac
     7501 tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc
     7561 agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa
     7621 gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag
     7681 tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag
     7741 ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggt
     7801 ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat
     7861 gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa
     7921 gtaaacaatg ttgatccggc gaagctgcaa gaatctagcc cgctgccggt tctcggcgct
     7981 gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat
     8041 gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc
     8101 gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc
     8161 gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc
     8221 ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa
     8281 cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct
     8341 ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg tatcgagaaa
     8401 gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact
     8461 tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt atcagctgac tgaactggcg
     8521 cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac cgttaaagca
     8581 gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag
     8641 atcaaccgtg ttgcagcgtc tcagggtgta gaactaggtg caggcattga aatcgttgat
     8701 ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa gaacaaaggc
     8761 atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg
     8821 ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc
     8881 atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg
     8941 ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat
     9001 ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc
     9061 ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc
     9121 gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg
     9181 atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg
     9241 ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt
     9301 aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg
     9361 cagggtatgc gcaaaccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc
     9421 tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatcca
     9481 cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt ttttattctc
     9541 ttaatttgca ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg
     9601 ccatctcact ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc
     9661 gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc
     9721 ttttttgttg ctattttaac cagtcttgcc acctgggtag ttccggtggg gatgtttgac
     9781 agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca
     9841 cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg
     9901 ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg gttaacctca
     9961 ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg
    10021 tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat
    10081 acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc
    10141 gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg
    10201 gtccggctgg gctatgacag tattaccacc gtcctggtga cttatattgc cacgcaaatc
    10261 ggttttgcca gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg gattgccggc
    10321 gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactctgatt
    10381 ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc
    10441 gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt
    10501 acctttggtg actggctggt attgattgtc ctgacagccg taatggtctg ggtgatttgg
    10561 ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt
    10621 ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg
    10681 gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc
    10741 gccaaaggga ttttgctgct ggttggtaat ggtgaagcag gtaatgccag cgtgttaaat
    10801 actatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt cgcggcctgg
    10861 tttatgttgc tcttccaggc tgtatttaat ttcttcgtga cgtccggttc tggtcaggcg
    10921 gcattaacta tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt
    10981 accgtgctgg cgttccagtt tggtgatggc ttcagccaca tcatttaccc aacctcagct
    11041 tcgttaatgg caacgctcgg tgtgtgccgg gtggacttcc gtaactggct gaaggtgggc
    11101 gcgaccctgc ttggactgct gtttattatg tccagcgtcg tagtgatcgg cgctcagttg
    11161 atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt
    11221 tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc
    11281 gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact
    11341 ccactgactt catcttcctg tagggcgaag ggaccgtgag agacgcaact gaacaacgcg
    11401 ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca
    11461 ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg
    11521 gcctgaacta caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg
    11581 gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga
    11641 tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct
    11701 tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa
    11761 ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc
    11821 gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc
    11881 agcatgccat aacccgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag
    11941 tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta caccagcaca
    12001 tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg cgtcaaaaac
    12061 agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcagcatc
    12121 atttgatcca cttcgctgtc gcagttgcca cgcacagcga taaccttatg tgccacttca
    12181 ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg
    12241 tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttggac aaacaactcc
    12301 acaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc
    12361 actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc
    12421 agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt
    12481 cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc ggttaatcat
    12541 tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc
    12601 taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct cggccgttag
    12661 tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc ggatgggcat
    12721 cagatcgctg cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag
    12781 cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc
    12841 agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc
    12901 gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg
    12961 aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag gggagaaaaa
    13021 gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg
    13081 ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgtaag
    13141 tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc tgcaatgatc
    13201 gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct cgaagaagca
    13261 gagcttgatt atcgcttgat taaggtagac ttggggaaag gcggtcagtt tcgtccggaa
    13321 tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc tcctgccgat
    13381 ggcggcgaac cgttaagcct ctttgaggct gctgccattt tgttgtatct ggctgagaaa
    13441 acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc
    13501 tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc
    13561 ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctttaccat
    13621 gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta cagcattgcg
    13681 gatattgcct gctggccgtg ggttaatgcc tggactcgcc tgcgaattga cctcgcaatg
    13741 tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac cgggcaggca
    13801 ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg
    13861 tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta
    13921 ttcgtataaa gaaccttcgt ttgcgtacgt ttatcggaat taaggaagaa gaaattaaca
    13981 accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa gcgcgcacta
    14041 gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt attcagcatg
    14101 tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac
    14161 gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct
    14221 acgccgattc ggtatccatg accttaagct ggcagcgtta atcgccatat cgggaggctg
    14281 catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga ttccgcgttt
    14341 gctggagctg ggccatcaaa ttacggtagt gacgcgtaac ccgcagaaag ccagttccgt
    14401 tctcggccct cgggtgacac tatggcaagg gcttgccgat caaagcaacc tcaacggcgt
    14461 tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga ctcacaagca
    14521 aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa
    14581 tgctagcgac acgccgccgt cggtactcat ttccggctcg gcaacgggct attatggcga
    14641 cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta ctcataaact
    14701 ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct
    14761 gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa tgctgccacc
    14821 gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg cctggattca
    14881 tattgatgat atggtcaacg gcattctctg gctgctggat aacgagctgc gcgggccatt
    14941 taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc tcggtcatgc
    15001 gctgcatcgc ccggctattt tgcgcgtccc cacaaccgcc attcggctgt taatgggcga
    15061 atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg
    15121 ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg
    15181 tttacagcaa acatccgcca gttaactccc tgtgttacag gattagtggc tttgcgcgat
    15241 aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta aacctctatc
    15301 cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc
    15361 acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc gttctgttat
    15421 ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt aattaaacag
    15481 ggcttttagc tgtctgtcat tagtgttccc tgtaactaac agcgaaacaa tttggtcgac
    15541 taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt
    15601 gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg gaaacgctga
    15661 tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc agagtgaata
    15721 aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg gaagctcttt
    15781 atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc
    15841 cattaattct tccggcttac tttggtgctc tatcactaca taaatataac ccactccctc
    15901 ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt catcaataaa
    15961 actgtttggt tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag
    16021 atgaatatta ataaaattcc gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc
    16081 atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag
    16141 atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta tctttggaca
    16201 gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga agctgactcg
    16261 caaattcccc ttgaaaatac tccggcagcg ttagccaccg gagaggaaat tatttcagcg
    16321 atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac tgctccggcg
    16381 cgccctcttc ttctattttc ccctgatgga ggaaaatgac gtgagtagaa acatggcgag
    16441 caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc agttgctgca
    16501 taatacgtaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt tcatcaaaca
    16561 gtaaaacttc tggttccatc gccagcgccc gcgcgataga aacacgctgt tgctgaccgc
    16621 cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact ttcgccagat
    16681 acttcaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt
    16741 ccatgacgtt ttccaacacc gtcatatggc tccagagatt gaagtgctgg aataccatcg
    16801 tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt tgaccgtctt
    16861 tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgatccttca ctcggttttt
    16921 cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc
    16981 ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttcg tgttcgccat
    17041 agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt
    17101 gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg
    17161 acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac
    17221 gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc cgtaaacgcc
    17281 agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa cgcaatacgc
    17341 agcgcagaag gcaaaataat gcagcgatac attttaaagg tcgagaagcc ataggctctg
    17401 gcggcttcaa tttccccatg cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg
    17461 taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact gcggaagaaa
    17521 gcgttaagga attcggttcc cttaacaatc tcaagcgtgt acatgccgga atagaacacc
    17581 agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca gattggaaac
    17641 tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag gactccgcct
    17701 atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa gcgataaccg
    17761 tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct
    17821 tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa acggtggtga
    17881 aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag gtacttttgc
    17941 ctgccagctg cgtggctttg accacatctt ccaggccgag taacgaaacc agtgcggtag
    18001 atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg
    18061 gaaacatgat ccgccgaaac acttgcccgc gagtaaaacc gaacgccgtc gccgcctcta
    18121 tatgcccttt cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt
    18181 agataaaacc gagagtgata ataccagcga ccatcggatc gatatcaatc tgcccgacgc
    18241 ccatcgcctc cgtcaccatg tttagcgcaa tctgcaaacc atagaaaatc agcagcatca
    18301 acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa
    18361 gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta
    18421 cagagctgat agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc
    18481 catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg
    18541 gttacccgaa gggagcgggc tttctttcac cgatggcgat taaccaccat aaacatcaaa
    18601 atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc
    18661 tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac
    18721 gccaaacagt ttttcatctt taacagacgg accaccgaat ttgtaatctt taccgacggg
    18781 ttgtttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat caatacgtcc
    18841 ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt caatgccttt
    18901 tggtgcccag tgttcattac cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg
    18961 tttgcctttc agcgactcga ctgtcggctg aatgtcagaa tttttcgcca ccaccagacg
    19021 agaatcggca gcgtacagtt tgtcggtgaa ggcaatttct tgctgacgtt tttccgtaat
    19081 ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc
    19141 cagcgggttt tcgacaaacg tacattgcgt attgatgcgc ttgcacaact ccttcgccag
    19201 ctcgatatcg aaaccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt
    19261 cgggtcggta ccgatgcgga tgttttgcgg aattgcagca agtgccgcag ttgcgctgga
    19321 gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag
    19381 tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc
    19441 aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc
    19501 atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca
    19561 ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa tactttttcg
    19621 ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg aaggcagccg
    19681 tcagttcagc ataatcttta cgtagcccta caccggtgcc atcaccgaag tattttttgt
    19741 cttttactga tgagccagca aaagcgaaat ctttaccagc aggttgcttg aggaatcctt
    19801 cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc agatcggaat
    19861 agaccaaatc ctggttggca taggccacca catccacgcc tttactacgc caggtctcgt
    19921 tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgaat
    19981 ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct gcggcgtaca
    20041 gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa
    20101 tagcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc
    20161 aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccaa
    20221 caaaatcacc tttagcatct ttcgacgaga acagcgcata ggtggtatcg gttccgatac
    20281 gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg actaataaag
    20341 acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt
    20401 gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat cttgacgaca
    20461 acagtaacat tcaacgttaa atatgttaat aagacattgc attattgtcc tgaagttgaa
    20521 gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg
    20581 aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc
    20641 tgccagcggg caaagagatc ttcaggaagg gttattgcaa actggtcaag aacacgatta
    20701 accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga
    20761 ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc
    20821 aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac cacatctgcc
    20881 gcacgggtca gtaagccatc agtatagcta tggacaatgc cggacagggt tttgattgaa
    20941 cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct ggcggaaata
    21001 tcgcgcgcat cgagcgtgac atcagctaat gcctgcactt cgcgcaggga aaaatccgtt
    21061 tcgagggata aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg
    21121 acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc accgctgatg
    21181 cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagt
    21241 atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc
    21301 ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt tggcgtcatc
    21361 attacgtaac gtatcgagga aatcgaggta gccctgatca acatctttgg tgacgtagac
    21421 gccgttgaac accgagcatt caaactgctg gatatccgga ttttcagcgc gaacggcgtc
    21481 gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc
    21541 atcaacttcg cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa taccataaac
    21601 gttcgggaag cgaatttccg gtgccgcaga agcgaggtac actttcttcg ctccggcttc
    21661 gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt cgtcgaccag
    21721 caggacgttt ttatcgcgga actcggcgcg gttggcattc agtttacggc gcacggactt
    21781 acgacgcagc tgctggcccg gcatgataaa ggtgcggcca acatagcggt ttttaacgaa
    21841 gccctggcgg tacggtttgc ccagaatacg agcaatttcc agcgcgatat cacacgaggt
    21901 ttctgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc
    21961 gcccagtttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt cgataaacga
    22021 gtccgggcgg gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc
    22081 acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac
    22141 gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag cgaccatata
    22201 ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa tcccgtttgg
    22261 atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat acgcgccgcg
    22321 gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca gcgggtagtg
    22381 gcggaagttg tccagctcgc tggcgaagat attaagcaga atttccgagt cggaagtggt
    22441 gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag cgttggtcag
    22501 attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg gctgcgcttc
    22561 agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg
    22621 caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa
    22681 acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg
    22741 aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac
    22801 aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg
    22861 caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaattg
    22921 cgggatcagc tgtgatttgc tccagtcttc acttttcgac accccggtaa aggagtcgag
    22981 aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac agacgcccag
    23041 cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca cgaagttcac
    23101 gatagcacca acgatcaggg tagcgataaa cagtaccgcg atggcaatcc catttcgaac
    23161 cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac
    23221 aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc cgtggatcag
    23281 gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg
    23341 tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa
    23401 cgtttgcgta gggatttcct tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa
    23461 cgccatcgac tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac
    23521 gccttatccg acctacggtt ctaacactgc ccaagcctga taagacgcgc cagcgtcgca
    23581 tcaggcaaga ctgtattaat tcggcgtata gcccattacc acgccactta agccagaaag
    23641 ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg gcccaaccag
    23701 aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc gataaccggc
    23761 accgcgcagc ttaccgacaa tctcattcac tttatcggca tttttcagcg cacccagttg
    23821 cacaacatag gctttacccg ttggcgctgc tttttcttcc acgaccggct tcggttctgg
    23881 cttcggcgca ggtggtactt caacctttgg tttaggcggc tccaccggtt tcggcttcgg
    23941 tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa tagtggccgg
    24001 atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc cttccggcgg
    24061 ctgcgtcggc aacgcctggg tggcggctgg catcatatca ggctcatcac gatcgcccgc
    24121 tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct gcccgtccag
    24181 cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca ctaaccgatt
    24241 ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca tgtgcgaccg
    24301 tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc attgcggcat
    24361 cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca agcagttgtt
    24421 ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca accacgcttt
    24481 tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata accgccagca
    24541 cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc gcatgtggat
    24601 tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa cgtcccggca
    24661 aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc ccgctggcac
    24721 gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac aagcggcaac ggcagatttt
    24781 ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa tagttccact
    24841 caacgccccg acgttgtaac agtgcacctt tttcctgcgc cacatcagca atggtagaag
    24901 gcatttccgg ctcaccgaca attgccggtt tttcgctgcg gaagatgcct gctttctcga
    24961 gaccaatact ttcgcgatct ggacccagcc agtcggtatg atccagcgca atactggtta
    25021 ctaccgcgac atcggcatcg acaatattgg ttgcgtccag acgaccgccc agccctactt
    25081 ccagaatcac cacgtcaagt tgtgcctgct tgaacagcca caacgccgac agcgtaccgt
    25141 actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca aaagaggcgg
    25201 tgtgggccga ttccggcaat tcctggccct gcacacgtac gcgctcggta taacgcacca
    25261 gatgaggcga actgtagacg cccactttgt accctgccgc catcagaatc gactccagcg
    25321 tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt
    25381 tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga tcgatagttt
    25441 tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg gcttgaggag
    25501 tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa aagggcagag
    25561 ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt accgggggta
    25621 ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc aacttcgcca
    25681 gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg atcgcgcctt
    25741 tctcgatcag gaattcactg cgctggaatc taggcggcag tttttcgcga acggtctgtt
    25801 cgataacacg cggaccggca aagccgatta acgctttagg ttcagcgatg ttgagatcgc
    25861 ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt
    25921 acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc atctgcatca
    25981 gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag atcagcgggc
    26041 agttatcttc ctgcgcctgc tcaacggcac gcacgaaacg tgcacccaca acagacccca
    26101 ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt ccatacagag
    26161 tgcctttcat caccaccagc gcatcttttt cgccggtttc tttctgcgca gatgccagac
    26221 ggtctttata cttcttggag tcacgaaact tcagctcatc tttcggctca agctcgctac
    26281 ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc gctgtcatac
    26341 gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca gcgcggtata
    26401 aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc gccttgcggg
    26461 tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt tctgtctgaa
    26521 cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc ccccaacaga
    26581 ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa agtggtcgaa
    26641 ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac ggattatttc
    26701 gatgacgccc ggcaaaatag aaaccacaat aatcccgacg atcagcagct taaggttatc
    26761 ctgaaccatc gggattgtac cgaagaaata gcctgcgtag gtaaaaagca gtacccacaa
    26821 cagtgcgccg atcacgttat aagcagcgaa atgacggtac gacatgtggc ccattcccgc
    26881 aacaaacggg gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa tcgttttgcc
    26941 gccatgtttc tcataaaact gatgggtttt gtcgagataa ctgcgacgga aaattttcga
    27001 attgggatta ctgaataact tttcaccgaa caaccgcccg atagtgtaat tgaccgcgtc
    27061 acccacaatc gcggcgatca gcatcagcac taccatcata tggacgttga gatcgttggt
    27121 ttccagtgat gccaacgctc cggcaacaaa cagcaatgaa tcacccggta aaaacggcat
    27181 taccaccaga ccggtttcac agaacaaaat taagaacaaa atggcataaa cccagacgcc
    27241 gtactccgcg actagctctg ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa
    27301 ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc tgtaactacg
    27361 ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga aatagcgggc
    27421 ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc agatacagcc
    27481 cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc aactctgcta
    27541 tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca atattcctga
    27601 ccatatgatg tacaaaggca ttcgctttga tatcaaccac cacataagga ccgtgacgcg
    27661 tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc accgcacgga
    27721 acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt
    27781 ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc agccgatgat
    27841 tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga aaatcatcag
    27901 gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt acgcccaagg
    27961 tccacgccgc gtctttgcgc tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt
    28021 gtacccctgc gtcagtacgc ccggcgcaga agacggtgat gggttcgttc gccacctggg
    28081 agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt tgccagccgt
    28141 aatacttact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc agttgttgct
    28201 ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta ctgccatcag
    28261 cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tggacttgct ccggcatacc
    28321 gtagtcatta cgcacgcagc caacagaaag atgcggcgta cccgaagcat cccctacctg
    28381 agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa acgcatcacg
    28441 cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg catgaccgta
    28501 gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct gcaggatttt
    28561 gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat ccggcaacaa
    28621 cggcagcata ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc
    28681 gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc cctgggcgga
    28741 ggctgaaatc aggctggtaa cactgatacg cgacaaaccg ccctgatcga ttaacggttt
    28801 cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat tccggttgcg
    28861 gtaatcggtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt cgggttcgag
    28921 agcaaacagt ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt cttcaaccca
    28981 ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa
    29041 ttcagcggca tcctgcacgg tgattgtttt accaccaaag cgcagttgtt cgcctgcgct
    29101 ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag ccagcgtttc
    29161 aagcagggct tcgcccatag cgccagttgc gcccaggacg gcaatgttcc agccttcaga
    29221 catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca gagagcatga
    29281 agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca cagcaatgat
    29341 gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg ctcaagatag
    29401 tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag cggtgcgtca
    29461 tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg atcgagcgga
    29521 ccatgcagcg taatgcggcc aaactcaggc gcgggcagca acgtatccag cgcaacgtgt
    29581 tgttcatgcc caataaactt gctataggct tcaaacactt gcgtcgtgcc acgcgcttta
    29641 ccctccaggg tatagcctgc gatatgcggc gtgccgatat ccacttttgt cagcaattcc
    29701 acattaagtt ccggctcgcc ttcccagaca tccagcacca cgcttaactt ctggccttca
    29761 ttcaggcagg tcagcagcgc ggtattatcg acgactgcgc cacggcaggc gttaatcaga
    29821 atcgctccgg gcttcaggct acggatcagt ttttcatccg ccagatgtag cgttttgtac
    29881 ggaccatctt taaagagtgg cgtatggaaa gtcagaatat ccgcgcgctg gactaactca
    29941 tccagcgagc ggaaatcacc ctcatcccca cggtcggcgc gaggcggatc gcaaagtaag
    30001 gttttaatcc ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc
    30061 acgatcccca ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag catcagcagg
    30121 gaggagaaaa catattccac caccgcaatc gcattacagc caggtgcagc ggaaaaacca
    30181 attcccgcct gcttcaacca tgcttcatcg acatggtcgg tccccgcagt ggcagttcca
    30241 acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac cgaacgcacc
    30301 atcagcgcat ccgcgtctgc cagttgagcg acggggattg gacgcccggg aaccgcgatc
    30361 acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc aacaaggatt
    30421 ttcacgtttg tgttacctgt atgagacgag agttaaccgg acaagtatgc cataatctcg
    30481 cggccaggca tacttgcgaa gatttcaggt ataaggatac gtaatgatac aacctatttc
    30541 cggccctcct cctgggcagc caccaggtca gggagataac ctgccgtctg gcgcgggcaa
    30601 tcagccttta tccagtcagc aacgtacttc gctggaaagc ttaatgacga aagtgacctc
    30661 actgacgcaa caacaaagag cagaactgtg ggcgggtatc aggcacgata ttggtctgtc
    30721 gggagattca ccgctgcttt cgcgtcactt ccctgccgct gagcataatc tggcgcaacg
    30781 tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat taggggagta
    30841 tttacgtctg gggaatcatc gtcaggcggt cacggattat atccgtcata actttggtca
    30901 gacgccgctg aatcagctct caccggagca attaaaaacc attctcaccc tgttgcagga
    30961 agggaagatg gttattccgc aaccacagca gcgcgaggcg accgaccgtc ctttattacc
    31021 ggcggagcac aatgcgctaa aacagctggt gaccaaactt gcggcggcaa cgggggaacc
    31081 cagcaaacag atctggcaat cgatgctgga actttccggg gtgaaagatg gcgagttaat
    31141 tccagcgaaa ctgtttaacc atctggtgac ctggctacag gcgcgtcaga cgctaagcca
    31201 gcaaaatacg ccgacgctgg aatcactaca gatggcgcta aaacaacctt tagatgccag
    31261 tgaactggcg gcgttatcgg catatatcca gcaaaaatat ggcctttctg cgcaatcatc
    31321 gctttcttct gcccaggccg aggatattct taatcagctt tatcaacggc gggttaaagg
    31381 gattgatccg cgtgatatgc aaccgctgct taatcctttt ccaccgatga tggacacgtt
    31441 gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct
    31501 ggtctggctg gttcgttaac cccgacgaaa tgacagtatc gtgacaataa tacccagcac
    31561 cgcagagatc gccccggcaa gaaataccga agagtaacca aacgtggtcg ccagcattcc
    31621 cgccagcggc ccggagacgc cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc
    31681 ggtgccgcga acttgtgagg ggacgcgttt aaccacctcc acgcccagcg caggaaagat
    31741 aagcgaacat ccggctccgg ttaacgccgc gcccgctaat gcgacccatg cacctggggc
    31801 ttgccagagc agcaacaagc ccaccgtttc tacaagcaga gagacaatcg ccactttcac
    31861 gccgccaaaa cggtccggca tccagccaaa catgacgcgc atcacgacaa atgcgccgcc
    31921 aaacgcggta agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac
    31981 gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca
    32041 gataagcccg acaacgctcc acagcgatgg acgttctccc gccagggccg gtactttgcg
    32101 cactgtgccg ttacaggccc acgccagtaa gggtaatacc attgtggtga tcgccagtgc
    32161 ggcaaaaccg taatggctat gaatcaacag gccaagcggc gcaccaacag cgagggcacc
    32221 gtaaatcgcc attccgttcc atgacatcac tttgccagag tgttttggcc ctacgatgcc
    32281 taacccccag gtcagagcgc ctgtcagtaa ctggctttca ccaaacccaa gaatcaaacg
    32341 cccgatgacc aacagggcaa atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag
    32401 caacgcgccg ccagccagac cacaagctaa catcccctga agcgccgaac gttttgcgcc
    32461 atattgatcg gccagtcgcc cggcgtaacc acgcgtcagc accgtagcca gaaactgaat
    32521 cccgacggca atgccgacca tggtattgcc atagcccaga tcatgatgaa caaacagcgg
    32581 gataaccggc aacggcaacc ctacggtcat gtaggtgaga aaaaccgcaa aagagatgcg
    32641 gaagagcgaa aaactggcag aagatcgtgt ttcggtttgg cttacagcag tcatgcatta
    32701 ctccagaatg cagcgcaagg cgaggagtat ccccgtctca tctctctggt ttcagggtta
    32761 cggtgcgttg gcaggattta acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa
    32821 gtttgcctag aactggtggc gattgtcaat gatgtgaaaa agggaaccat caggttccct
    32881 tttgcgttag tgccggaggc cgcattggcg cgtaacgtcg gatgcgacgc tggcgcgtct
    32941 tatccgacct actccggcct actgcgaatt aatcttttca gcttgcgcat taccagcgtg
    33001 gcgttggtgc cgccgaagcc gaagctgtta gacataacag tggtcagttc gcgatcggtc
    33061 gtttcggtca cgatgttcag acccgcagcc tgctcgtcca gctcttcaat gttgatgctc
    33121 ggggcgataa agccgtgttc cagcatcagc agagagtaga tagcttcctg cacacccgca
    33181 gcgcccagtg agtgaccggt catggctttg gttgcagaaa tcgccgggct cttatcgccg
    33241 aacacttcac ggatagctgc cagctctttc acgtcgccaa ccggagtcga agtaccgtga
    33301 gagttcaggt aatcgattgg ggtatcaacg ccatgcatcg ccatctgcat acaacgtact
    33361 gcgccttcgc cagacggagc aaccatgtct gcaccatcag aggttgcgcc gtagccaacg
    33421 atttcagcat agatgtaagc accacgcgcc agcgcgtgtt ccagctcttc aaccactacc
    33481 ataccgccgc cgccagcgat aacgaaaccg tcacggtgag cgtcgtaagt acgggaggct
    33541 ttttccgggg tgtcgttgta tttagtagat agcgcaccca tcgcgtcgaa ttcgcaggcc
    33601 atttcccagc acagctcttc gccgccgcca gcaaacacga tgtcctgttt gcccagttgg
    33661 atctgctcta ctgcgttacc gatacagtgt gcggaagtcg cacacgcgga gctgatggag
    33721 tagttaacgc catgaatttt aaacggcgtg gcgagacagg cagaaacgcc ggatgccatc
    33781 gctttagtga ccacatacgg gccaaccgct ttcaggccgc gcgggccgcg cattgcgtca
    33841 gcgccgaaca cctggaaacg cggggaaccg ccgccggaac ctgcaatcag gccaacgcgc
    33901 gggttattct ggtaagcttc cggagagagg cccgcatcag cgattgcctg ctccatagaa
    33961 aggaatgcat aaatggatgc gtcgctcata aagcgcacaa ctttgcggtc aatgaggcca
    34021 gtggtatcca gttttatgtt gccccagacg tggctacgca tgccggaatc cttcagctcc
    34081 tgagagaaag tgatccctga acgtccttca cgcagagatg ccaggacttc ctgctggtta
    34141 ttaccgatgc tggaaacaat gcccaggcca gtaatcactg cacgtttcat tcaatacctc
    34201 tgtaagtcgc acatagagta agtttcgaat gcacaatagc gtacacttgt acgccgaaca
    34261 agtccgatca gccatttaat agagatattt gcgcagcctt acacacatcg ctaagatcga
    34321 gccaccgcct gtaagacgag taacttacgt gaaacactac tccatacaac ctgccaacct
    34381 cgaatttaat gctgagggta cacctgtttc ccgagatttt gacgatgtct atttttccaa
    34441 cgataacggg ctggaagaga cgcgttatgt ttttctggga ggtaaccaat tagaggtacg
    34501 ctttcctgag catccacatc ctctgtttgt gatagcagag agcggcttcg gcaccggatt
    34561 aaacttcctg acgctatgga aggcatttga tcagtttcgc gaagcgcatc cgcaagcgca
    34621 attacaacgc ttacatttca ttagttttga gaaatttccc ctcacccgtg cggatttagc
    34681 cttagcgcat caacactggc cggaactggc tccgtgggca gaacaacttc aggcgcagtg
    34741 gccaatgccc ttgcccggtt gccatcgttt attgctcgat gaaagccgcg tgacgctgga
    34801 tttatggttt ggcgatatta acgaactgac cagccaactg gacgattcgc taaatcaaaa
    34861 agtagatgcc tggtttctgg acggctttgc gccagctaaa aacccggata tgtggacgca
    34921 aaatctgttt aacgccatgg caaggttggc gcgtccaggc ggcacgctgg cgacatttac
    34981 gtctgccggt tttgtccgcc gcggtttgca ggaagccgga ttcacgatgc aaaaacgtaa
    35041 gggctttggg cgcaaacggg aaatgctttg tggggtgatg gaacagacat taccactccc
    35101 ctgctccacg ccgtggttta atcgtacggg cagcagcaaa cgggaagtag cgattatcgg
    35161 cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta cggcgcggct ggcaggtagc
    35221 gctttattgc gcggatgaag cccccgcgct gggtgcttcc ggcaatcgcc agggggcgct
    35281 gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc tttttctcta atgcgtttac
    35341 ttttgctcgt cggttttacg accaattacc cgttaaattt gatcatgact ggtgcggcgt
    35401 cacgcagtta ggctgggatg agaaaagcca gcataaaatc gcacagatgt tgtcaatgga
    35461 tttacccgca gaactggctg tagccgttga ggcaaatgcg gttgaacaaa ttacgggcgt
    35521 tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt tggctgtgcc cagcagaact
    35581 gacccgtaat gtgctggaac tggcgcaaca gcagggtttg cagatttatt atcaatatca
    35641 gttacagaat ttatcccgta aggatgactg ttggttgttg aattttgcag gagatcagca
    35701 agcaacacac agcgtagtgg tactggcgaa cgggcatcaa atcagccgtt tcagccaaac
    35761 gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc catattccga caacgccgga
    35821 actggcagag ctgaagcagg tgctgtgcta tgacggttat ctcacgccac aaaatccggc
    35881 gaatcaacat cattgtattg gtgccagtta tcatcgcggc agcgaagata cggcgtacag
    35941 tgaggacgat cagcagcaga atcgccagcg gttgattgat tgtttcccgc aggcacagtg
    36001 ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc ggtgtgcgtt gtgccacccg
    36061 cgatcatctg ccaatggtag gcaatgttcc cgattatgag gcaacactcg tggaatatgc
    36121 gtcgttggcg gagcaaaaag atgaggcggt aagcgcgccg gtttttgacg atctctttat
    36181 gtttgcggct ttaggttctc gcggtttgtg ttctgccccg ctgtgtgccg agattctggc
    36241 ggcgcagatg agcgacgaac cgattccgat ggatgccagt acgctggcgg cgttaaaccc
    36301 gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg gttaaggcgg ggtaatctgc
    36361 tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc aacctacgtg tgacgtgtag
    36421 gcctgataag acgcgataag cgtcgcatca ggcatggtgc tccagacgcc gcaacttaca
    36481 gctgtgacgc ctgctgaaac aggttttccc acatatcggt caccagcgcc tggtcacgcg
    36541 gcgacaattc accggcacca atggcttttt ccagactctg gctaacggtc gtatgcaccg
    36601 cctgagcgga gtggtcatca ccactttcca gttctgcgat ggctaatgtc aggtggccac
    36661 gcaaataccc actggcaaac aactcatcat cactggcatg gtcaaccatg ccgtcgatta
    36721 atgccagaat gcgtgattca aactccgcga tcatcttctt tcctcattgt aaaacgtggc
    36781 gctggcttca gttgagcgct tccggccacg ggaactgttc cgccgtaagt tccggcgtgt
    36841 gataataatt ttgtagtgct ttaatgaagc gtgccgggcg ttccggaatg ccatttgtca
    36901 gataatccat cacctgcgca tgtacccgcc gttgaaacac cacacgatcc ggttcgaaat
    36961 ccccttccag attgtcgcaa ctgacgttaa acggatatcc agccgccacg cagaataacc
    37021 agtcgaacgc ctgcggcttc acttcaacat cttcaaactg gctttgcgtc tgggcatcgc
    37081 gtccgtccgg gcaataccag tagccgaaat caaccagctc acggcgcgct tttccggcaa
    37141 tacaccagtg cgaaatctca tgaattgcgc tggcataaaa gccatgagca aagacgattc
    37201 ggttatacgg tacttccgca tcagcaggaa gatagatcgg ttcgtcgtcg cctttaatca
    37261 gacgggtatt aaattcatcg gcaaagcagc tattaaaaat ttcaatcaac tgctcgtagt
    37321 ggtgtgtact gttcattagt tcatccccaa ccagtggagg atctcctgtc cgtggctatc
    37381 gtaaagtaat ttggcactca tcaccgccga gacgataaca atcatcgggc ggatcagctt
    37441 ttgtcctttg ctcaacacca accgtgaacc catgcgcgcg ccgaggaact gccccaccag
    37501 catgacaaag cccgtcgccc aaatcacttt gccgccgaga ataaacagca gcagaccgcc
    37561 aatgtttgac gttgcgttga gtaatttggc atgagccgtg gctttggcga ggttgaatcc
    37621 gcacagcata acgaaggcca gcgcgtaaaa cgatccggca gccgggccaa agaatccgtc
    37681 ataaaaaccg acgcagccac cggcaattaa cgcgaagggt aaaccgtaca tccggcgctg
    37741 gcgatcttct tcacccagct ttggcatcag caagaaatag aggccgatac aaatcaccag
    37801 aatgggcaaa atctgccgta agacatcagc ctgaacgtac tgtaccaaca gtgcgccgct
    37861 cattgagccg acaaaggtca tggcaatatt gagtttctga tcgcttaaac taaccacttt
    37921 gcggcgaata aagtagatag tggcggaaat agagccgccg caggcttgca gtttattggt
    37981 tgccagcgcg ttagcgggag acattcccgc agccatcaat gccggaatgg tgagtaaccc
    38041 accgccaccg gcaatcgagt cgataaatcc cgccagcatg gcgacaaaaa agagaactcc
    38101 cagcaacagc ggagaaacca taaacaggct attaaacgtt tccattagat cacgtgctca
    38161 tccagtagcg cctggcagga aggcggcaac ggaggcggtg tcttcttctc aggctttgtt
    38221 gttcccggtt ttggaggttc aaaccagctt tgcagttctg ccccgcaacc atcgcctgat
    38281 ggcggtaaag gttgatcttc acactccaga ctatcggcag ggcaacgtaa tcgtacatgc
    38341 atatgcgcgc gatgctggaa ccagggtcgc actttgcgca accagtcgcg atcggtgccc
    38401 gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa aaatgcgcgt gacgtctttg
    38461 tcctgggcgg cgagtttgat caagctgaaa atttctggct tccacagcgt ggagacaacg
    38521 tgtttaccgt cgcgggaaac taagtccagt gcttgcgggc gcaagagctg cgcggaggtc
    38581 cagcgagttt tcggcagttg cagaaagata tcgacatcca gtccggtctg gtggctggca
    38641 tgaccgccgt tgaaacgccc accagcgggc atccccatat cgccaatcag caccgtaccc
    38701 atgcccagat tgctcacctg gctactcaga cgctggataa acatcaccag atccgggtga
    38761 ccgaaatagc gacgctgatc ggtacgcatg acctgataat gttcggactg tatcggcaac
    38821 gtgtcagcgc cgacaataca gccattagaa aaactgccta tcgattgtgc gctacccggc
    38881 acaggttggg ttattttttg ccacggcgtc gctgccaggc tagcactact ggcaagcaga
    38941 gccagcagcg caatcgcggt tttgttcatt ttttaccagc gtggaatatc agtcttcaca
    39001 tcggcatttt gcgcccgctg ccgtaacagg tgatccatta aaacgatcgc cagcatcgct
    39061 tcagcgatcg gcactgcacg gatcccgaca cagggatcgt gacggccttt ggtgatcatc
    39121 tcaacttctt cgccaaagcg gttaatagtg cgccccggca cggtaatgct ggaggtcggt
    39181 ttcagcgcca tatgagcaat gatttgctgc ccgctgctga taccgccgaa aatgccgccc
    39241 gcatggttgc tctggaaacc gtctttggtg atttcgtcgc ggttctggct gccgcgcagc
    39301 gccactacgt caaaaccgtc gccaatttcc acgcctttca ccgcgttgat gctcatcagc
    39361 gcatgggcga tgtcggcatc caggcggtca aagaccggct cgccaagtcc ggcggggaca
    39421 ccactggcaa caacggtgac tttcgcgccg atggagtcgc cctctttttt cagcgcgcgc
    39481 atcagttcat ctaacgcgtc gattttgtcg gggtccgggc agaagaacgg attttgttca
    39541 accagcgacc agtctttgat ttccagcgga atgtcgccca tctgggtcag gcagccgcgg
    39601 atttcaatac caaatttctc ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc
    39661 atggcggttt cgcgggcgga agaacgtcca ccgccgcgat aatcgcgcag accgtatttt
    39721 tgttcgtagg tgtaatcagc atggcctgga cggaagacgt ctttaattgc gctgtaatcc
    39781 tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc tggtgccagt agtaacgcct
    39841 tcaaaaacac cggagagtat tttgacctga tccggctcgc ggcgctgggt ggtatagcgc
    39901 gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt ccgcttccgt cagcggaatg
    39961 cctggcggaa caccatcgac gatgcagccg agcgccagcc cgtgcgattc gccgaaggtg
    40021 gttacgcgaa agagttgtcc aattgtgttt ccagccatca cggctccgtt attgttgtgt
    40081 ttgcgtgttt acttaatctt tataaatcgc gaaatgttct cgtgcggcaa taagctgctc
    40141 tttagtgagc ataaacacac catcgccgcc gttatcaaac tccagccagg tgaacggaac
    40201 atcgggatat tgttccataa gatgtaccat gctgttgccc acttcacaaa tcaacacgcc
    40261 atcatcagca aggtaatctg ccgcgttacc gagaatgcga cgcgtcagtt tcaggccgtc
    40321 ggtgccagat gccaggccca gttccggctc gtgacggtat tcgtttggca ggtcggacat
    40381 atcttccgcg tcgacatacg gcgggttagt gacaatcaga tcgtattgca ctttcggcaa
    40441 atcacggaac agatcggaac gaatcggaat gacgttgtgg atcagaccgt gttcttcgat
    40501 gttctgttcg gcaaccgcca gcgcgtcggg agagatatct accgcgtcga cttccgcttc
    40561 cgggaaggca taagcacagg caatggcgat gcagccgcta ccggtacaca tatctaaaat
    40621 atgctgcggt tgcttgctga tgagtccggc aaatttattg ttgatcagtt caccaatcgg
    40681 cgagcgcggc accagcacgc gttcatcgac gtaaaattca tgaccgcaga accaggcttt
    40741 gttggtcagg taagccaccg gaatgcgctc gttgacgcgg cggatcacgc gttcaacaat
    40801 acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata tcttccggga tatccagcgg
    40861 caggtagagc gatggtaaca ccagctgtac ggcttcatcc cacgggttat cggtgccgtg
    40921 accgtaccag atatttgccg cgctgaagcg gcttaccgac cagcgcaata tgtcctgaat
    40981 ggtttgcagc tcatttactg cttcatcaac gaaaatttta tccacgtatt cctccagggc
    41041 atgatcgcaa taatttcggc ggctagtttg ccatgaagat gacgataaat cagcattcac
    41101 gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt gtgctacgag tcgggtaaac
    41161 tgtaggaaaa ttagaaatag agacagataa atgaaaaaga aaacaacact tagcgaggag
    41221 gaccaggctc tgtttcgcca gttgatggcg gggactcgca agattaagca ggacacgatt
    41281 gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg tgaaacgctt gatccaggag
    41341 caggctgatg ccagccatta tttctccgat gagtttcagc cgttattaaa taccgaaggt
    41401 ccggtgaaat atgttcgccc ggatgtcagc cattttgagg cgaagaaact gcgccgcggc
    41461 gattattcac cggaattgtt tttggattta cacggtctga cgcagctgca ggccaagcag
    41521 gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg tgttttgtgc ctgcgtgatg
    41581 catgggcacg ggaagcatat tttgaagcaa caaacaccgc tgtggctggc acaacatccg
    41641 catgtgatgg catttcacca ggcaccgaaa gagtacggcg gtgatgcggc tttgttggtg
    41701 ttgattgaag tggaagagtg gttgccgccg gagttgccct gaatgctggc agcaaatgcc
    41761 ggagcagcgt aaacgcctta tccggcctac aaatatgtga taattcaata aattgcaatt
    41821 cacttcgtag gcctgataag acgcatcaga gcaatactac gaacttcagt tcattcaata
    41881 catactgaaa acgacacgtc gaatcaatcg gttgcccact actggtattc acattcacca
    41941 caatatcgtg tagtacttta tccgttaccg gatgacgagt tggcgtaacc gttaccgttg
    42001 atttatcaca accgtaagag gtaaaggtta acaattctga taccgcgcgg cagtatgctc
    42061 ctctgccatc attatcagaa aaattactgc catcacagag tctgccgcct tccatcaggt
    42121 aggtcgaaag cgtggtggtc actgttgacc ccgtggactg aattcgtaac gccttattat
    42181 ggctgacata attttcactc caggagatat cgtatgaagc gaccggtatc gttgggcata
    42241 ttgtgttatg cactgtaggg gccgtatagc ttccaaatcc gggtaaatct gtataggaat
    42301 aaaaggatgc gatccaaggg caaccataat catcagatgc aacggtaatt ttaaacgtca
    42361 tatatgcaca acgaaaaatt ttcgtatcta tgccagggca atttgagtta ttaattgtat
    42421 caaagtcagt cagtaccaaa gttggagctg aacccgaccc agaccaggaa ataacccgca
    42481 acgtatcgcc tttatacgca aaagaatcat atttcccata tgcaacctcc tttggcgtta
    42541 tcaccgtaac attgaaaaca ctgctcgcta cagcaccggt taccttgtcc atggcataat
    42601 aaactctcac actgtcaaaa ctcgcactca taagtttatc tccagcataa ctcttagatg
    42661 cgccgacaac actcagaacg agcagcatta ggctcgcgaa aattatggtc cattttttca
    42721 tttcagaaat actcaaaggt tgccgtcact gtggcactaa agtctccgga cgttacatct
    42781 cgcccgctct ttgcctgtaa ccaggtatta aaatttaggc tgttatttcc ctgattcagc
    42841 gggagcgtga cgcctgttgt attgttgatt ggcaccgatg tgcctgccgc agtttcaata
    42901 ccaatcccag cgccggatgc tgatgacgag gagtcaaagg ccagaaagcc gggtagagca
    42961 ctatcttctg ttcctgtcaa cgttactttg acattaaaca atgtagaact atgacaatct
    43021 ttcaattgaa acaccaatgg cactcgctct gactgcccaa ggttcattaa atcactggcg
    43081 gcaatggcag gaaaatgtac ttctgccaac tggctaccat taatcactgg cgtacacgat
    43141 ttactgatta aattgccggt aaagtgcaaa ttatctcctg ctattgtggt tccactgccc
    43201 agcaacatca ggctactaat aaaaaacaac gtacgctttt tcttcattgg taatctacct
    43261 gtaacgtggc ccaggcctca aagtccgcct ctggcagaat gacaccactc ttttttactg
    43321 gcacagccgt taacaccggt aaatcggttt cattcaccac aagtggcgta ttgatcgtaa
    43381 aactctgccc tgcctgtttg agctgaatgc ctaaaccagt gatattgctg cttactgcag
    43441 cattatcaaa atcgcttggt gtgccactcc agctcaaggt catttccatt aatgggtcgc
    43501 gaaccgccgt atcgcaggta atggaatacg gcacggtttg ctcataattg ctgccattaa
    43561 tgtcatcaat aagcacatcc tgaaaagtta tttcaatggt ttgagcgttg ctaatcgtgc
    43621 agttaggcgg ctgcactaaa taaccgtgaa aagagacgtt tgagagtgcc atcgagcaaa
    43681 agccccacag caaaataagc gagatccgtt tcattggtaa tccaccacaa ttgtggcact
    43741 ggcgttaaat tcagctgccg ctaacgttgt accactctgt ttcaccggca cagcttccag
    43801 aacgggaact cccgaggaga aattaaatgg caaccatgag ccagaggtta agtcgagtat
    43861 tgtgtgatca ctacttttct gaacccgaat accaaaccca ggaatacccg tgctgataac
    43921 tgtttcaccg ttaatcacga ccgttgtcgc ctgcatctgt agacgcaggt catccattgc
    43981 cagattgtta caaacaagcg agtagtcaat gggacgttta taatcaaccc tattgatctt
    44041 attaatgaat acattaccaa actcaactgc cgcaccattt accgtacaag gaggaggggc
    44101 atcgacaagc acccgtaaat tcatacttac tgacttactc gctgcaactg caaagccgct
    44161 accaacacaa catatcagta acccagttac cgcccagaca gaacaagatc ctttcatact
    44221 ggctctcccg gcctcagcct ttgcggctct gctcttcatc gacctagcaa ctgttaccgt
    44281 tgcaagtgaa aaagagcggc atccgagcgc catagtcgtt gacataggtc agtaccggca
    44341 cgctacccat cttcacattc agcggtgcag aggttttcgg cgacatcact attggcgaga
    44401 aaccggctgc cggattgcca tttttctgtg tgctggcatt actgaaaatg acgtaatacg
    44461 acgtcgggtt attgacggta aattcctgac cgttgcgggt taaagtaatc ttatattgct
    44521 aggggttttt catgctgacg ttctccagcg ccttcggtcg ccagaacaac ttaatacgcg
    44581 tttgtagtgc tatttgcagc gtgttggctt tgtttgattt tggcgggatc tcacgcacgt
    44641 tgaagtagaa caggctttca cgatcggcag gcaatttgtt gatatccggc attccctgca
    44701 cttttacctg cccgttcatc attgaatcga tgcgttgcac cggtggcaga acagttaacg
    44761 gtgaactgat cttgttaccc ttttcatctt cgatccagct ttgggccaga taaggcagtt
    44821 tcggatcgtt attgcgcaac gtcacgctga ttgatttgtc gctttcatta aatatcaaac
    44881 gggtacgatc tggcgttaca gaagcctggg cagacaaact ggcagcgcta agcagtaacg
    44941 ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc agacatggat cattccttaa
    45001 tcacgttatt ggttagctga aacagaaaca gggggattgg tgggtgtcac ctgttgcgtc
    45061 tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg gcgcgacgtt gcctttatgc
    45121 tggcatggca gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg caggttgata
    45181 tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt cgccaggttt cactccagcc
    45241 agataaacat tgccgtcatc atcgacaagg cccactgtct gctcgttatc attttttact
    45301 tctgcgccaa acggaggatg gctgccgtct tgtaaacgca gcaccgccat cgctttttga
    45361 ccactgatga cggcaaattt gcggtagcca atcgcccctt cagttagcgt ggcttgtacc
    45421 accgactggg ttgcttcggc gttttccggc agtttgttga ggtcgatata cgcctgattg
    45481 cggtaatagt tattgacgtc agaaacgacg gctttaccaa acatattggt ataaacagca
    45541 gcaccgttac cttctaccgg aacatcggca acgccatcgg catcaatcaa caagcgtgtc
    45601 ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc
    45661 tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt agttcgcact gaggtcaacc
    45721 tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg
    45781 ccgacgttca actgatagtg agtcgcgtca tcgacacggc tgaaataacc gacctgactg
    45841 ctgtccgtcc cactgccata gtaccgttat agctaacggt actgttgtcg ccccacggca
    45901 tactgagcga aatgtacatg cctttgtcgg cctggttgtc atactcgtaa cggtagccag
    45961 tcatcgagat gctgacatta cgaatactgc ccatattgaa atagtgcgag agcatgatgt
    46021 tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg ggtatagttg agataaaccg
    46081 aaacacccgc atcgcggaag ttctggttat aagtggcggt gtacatctct ttgtcgttgc
    46141 ccgtgcgtac cattccgctg tcactggcat ccagatactc gctcatggtc ataaagttct
    46201 cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt gagctggtca aagtctttgg
    46261 aataactcac acggaaggag ttaccgtcca gcgaaccttt gccataagcg gtgtctttat
    46321 ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc gccgaatgta gacaaatcgc
    46381 gaccgacacc aagcgccgca gactgatagt tttcatctcc cagtgcgccg ccatataacg
    46441 accagccgtt agcgatcccc caggaagctt cagcaccaga aaaaaattca ccctcgacat
    46501 ggtgtcccca ctcttgcgga cggcccatca tgatcttata gcgaacctga cctggacgag
    46561 tgaggtatgg catcgaggcg gtgctgatgt catattcctg cacctggccg ttctgttctt
    46621 caatgcgaat atgcaacgta ccggagacag aatcaccaag atcctgaata cgaaacggtc
    46681 cagccggaac ctgcgtttcg taaatcacac gccccatctg gctgacggtc acttttgctg
    46741 tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg cagattggga ggcaacattt
    46801 gatcgtcagt actgacactg ccaccaacat agttaaaacc atcaaaaata tcggatctga
    46861 ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg taacgcccgc ctggcatagt
    46921 agcgactcca ctcccatttt tttgagtttc atcgccgctg aattcatcgt catcattact
    46981 gcgagtatgt tgatagttag tctgccagtc agcacgcata cgccacggcc ctaggttaac
    47041 cccaaccgtc ccgttgccgc tgatctcgtt actatcatca ccgccatttt cttcgtgccg
    47101 ggtttgtgcg ttgatgctgt agtccgcgac gatcccggag atgccgtcat cccaacgtga
    47161 aggcggatcc ctgtcgggat aagtatattc gaggtaagcc tgtggcagtg aaatgactaa
    47221 tgcggactgg cttaaatcag ccttaatttc catgccttcc agttgaccgg atttcaggca
    47281 tttagcatcg tggctccatt gcagattttt cgccacgtct tcttttaaac caaactgcgc
    47341 taccagttcc ggtgtcagac aagcatagct tttgctcgcg tcatcttcac cagcatacca
    47401 gtaaatatcg tactcttccg ccaatggctg tttatttagt tgaacctgta aattgtattt
    47461 tccgggctca acatatccct ggctggaaaa acgcttcata tcaatttttg tgtcgccttt
    47521 taattccaga aaacgggaat caaactgaat gtcgtcttca gcccagacac tgctataact
    47581 ccctgacatt gccagcgcaa tgcaccacgg aagaatccgt aatcgaaaaa gagaatggtc
    47641 aggcatacac gtttctatcc gtttttattc ttgttcggtc aaaatacatt taaatccccc
    47701 atagcagtgt cgctatgggg gtatgcctta caggtaagta atctggaagg tggtatttgc
    47761 ttcaaaatta cccagatctg gcgcatcagc tgcgccgacc agccaggcgt taaagttcag
    47821 tgtctgcttc gctttgcctt tgttggtaga agtgtctttc actattttct ggtcaatacc
    47881 catgccgctt ttgtaagagg tacccagaga gtcaccgatc gccaggctaa cattgttaaa
    47941 tgccgcaccg gtatcggtat tgaaaatggt gtaataattg ccgctatttg cagaagaaac
    48001 ggtaccagta aaggtagtgg tcattgtttc ctgagtatca aatacgcaat cttgcagacg
    48061 aatctggaaa cttttcggcg tggtggtgcc gttatttttc agtttttcgg caccgatatc
    48121 acccatatca acttccagtt tgtgatcgtc cggaacgata gaacacactg agtcttcaat
    48181 ggtgccgtaa aaacgtgcag taccgttgtt acccgcagcg attgttgccg tagaggtgaa
    48241 cacgcccatc accattgccg cagcaatagc tgttttaaca aacttactca ttatttgtcc
    48301 ttaaactata aataattaaa aatataaaca ttgcaataca ttgattcagt caatagccaa
    48361 tgtttatacc cagccattga atacggcatg cgacccaaaa agaacaggtc gtagaaaatt
    48421 gtattcattg gtgtggggcc gcatagtgat gtttccacac ccctgat
//
LOCUS       DAZPDE010000011        48321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPDE010000011 DAZPDE010000000
VERSION     DAZPDE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.011"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACHU0J_000550"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACHU0J_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1215383.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHU0J_000551"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACHU0J_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR1215384.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="ACHU0J_000552"
     CDS             complement(1922..2374)
                     /locus_tag="ACHU0J_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1215385.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHU0J_000553"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACHU0J_000553"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1215386.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="ACHU0J_000554"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="ACHU0J_000554"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1215387.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHU0J_000555"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACHU0J_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1215388.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHU0J_000556"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACHU0J_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1215389.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHU0J_000557"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACHU0J_000557"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1215390.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHU0J_000558"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACHU0J_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1215391.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHU0J_000559"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACHU0J_000559"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1215392.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHU0J_000560"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACHU0J_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1215393.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHU0J_000561"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACHU0J_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1215394.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="ACHU0J_000562"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="ACHU0J_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1215395.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHU0J_000563"
     CDS             complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACHU0J_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1215396.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="ACHU0J_000564"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="ACHU0J_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1215397.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="ACHU0J_000565"
     CDS             complement(15085..15582)
                     /locus_tag="ACHU0J_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1215398.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="ACHU0J_000566"
     CDS             complement(15579..15941)
                     /locus_tag="ACHU0J_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1215399.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="ACHU0J_000567"
     CDS             complement(15931..16278)
                     /locus_tag="ACHU0J_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1215400.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /gene="yidI"
                     /locus_tag="ACHU0J_000568"
     CDS             16387..16800
                     /gene="yidI"
                     /locus_tag="ACHU0J_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1215401.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="ACHU0J_000569"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="ACHU0J_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="ACHU0J_000570"
     CDS             complement(18324..20039)
                     /locus_tag="ACHU0J_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR1215402.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="ACHU0J_000571"
     CDS             20206..21099
                     /locus_tag="ACHU0J_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1215403.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="ACHU0J_000572"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="ACHU0J_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="ACHU0J_000573"
     CDS             complement(21910..23526)
                     /locus_tag="ACHU0J_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1215404.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="ACHU0J_000574"
     CDS             23821..24495
                     /locus_tag="ACHU0J_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1215405.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="ACHU0J_000575"
     CDS             complement(24534..26195)
                     /locus_tag="ACHU0J_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1215406.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACHU0J_000576"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACHU0J_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1215407.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACHU0J_000577"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACHU0J_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1215408.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="ACHU0J_000578"
     CDS             27649..27981
                     /locus_tag="ACHU0J_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1215409.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="ACHU0J_000579"
     CDS             complement(27983..29215)
                     /locus_tag="ACHU0J_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1215410.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="ACHU0J_000580"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="ACHU0J_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1215411.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="ACHU0J_000581"
     CDS             complement(30377..31714)
                     /locus_tag="ACHU0J_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1215412.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACHU0J_000582"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACHU0J_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACHU0J_000583"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACHU0J_000583"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1215413.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACHU0J_000584"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACHU0J_000584"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1215414.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACHU0J_000585"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACHU0J_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1215415.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /gene="yidX"
                     /locus_tag="ACHU0J_000586"
     CDS             35377..36033
                     /gene="yidX"
                     /locus_tag="ACHU0J_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR1215416.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACHU0J_000587"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACHU0J_000587"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1215417.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="ACHU0J_000588"
     CDS             complement(37005..37403)
                     /locus_tag="ACHU0J_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1215418.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACHU0J_000589"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACHU0J_000589"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1215419.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACHU0J_000590"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACHU0J_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1215420.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACHU0J_000591"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACHU0J_000591"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1215421.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACHU0J_000592"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACHU0J_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1215422.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="ACHU0J_000593"
     CDS             43964..44044
                     /locus_tag="ACHU0J_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215423.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="ACHU0J_000594"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="ACHU0J_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1215424.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="ACHU0J_000595"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="ACHU0J_000595"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1215425.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="ACHU0J_000596"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="ACHU0J_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1215426.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="ACHU0J_000597"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="ACHU0J_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1215427.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="ACHU0J_000598"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="ACHU0J_000598"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1215428.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DAZPDE010000012        48083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPDE010000012 DAZPDE010000000
VERSION     DAZPDE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.012"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(155..3304)
                     /gene="acrB"
                     /locus_tag="ACHU0J_000599"
     CDS             complement(155..3304)
                     /gene="acrB"
                     /locus_tag="ACHU0J_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1215429.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3327..4520)
                     /gene="acrA"
                     /locus_tag="ACHU0J_000600"
     CDS             complement(3327..4520)
                     /gene="acrA"
                     /locus_tag="ACHU0J_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1215430.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4662..5309
                     /gene="acrR"
                     /locus_tag="ACHU0J_000601"
     CDS             4662..5309
                     /gene="acrR"
                     /locus_tag="ACHU0J_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1215431.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5437..8799
                     /gene="mscK"
                     /locus_tag="ACHU0J_000602"
     CDS             5437..8799
                     /gene="mscK"
                     /locus_tag="ACHU0J_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1215432.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(9011..9172)
                     /gene="rsmS"
                     /locus_tag="ACHU0J_000603"
     CDS             complement(9011..9172)
                     /gene="rsmS"
                     /locus_tag="ACHU0J_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1215433.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9186..9713)
                     /gene="priC"
                     /locus_tag="ACHU0J_000604"
     CDS             complement(9186..9713)
                     /gene="priC"
                     /locus_tag="ACHU0J_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1215434.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9783..10160
                     /locus_tag="ACHU0J_000605"
     CDS             9783..10160
                     /locus_tag="ACHU0J_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1215435.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10313..10864
                     /gene="apt"
                     /locus_tag="ACHU0J_000606"
     CDS             10313..10864
                     /gene="apt"
                     /locus_tag="ACHU0J_000606"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1215436.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10993..12918
                     /gene="dnaX"
                     /locus_tag="ACHU0J_000607"
     CDS             10993..12918
                     /gene="dnaX"
                     /locus_tag="ACHU0J_000607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1215437.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12251..12315
                     /gene="dnaX"
                     /locus_tag="ACHU0J_000607"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12971..13300
                     /locus_tag="ACHU0J_000608"
     CDS             12971..13300
                     /locus_tag="ACHU0J_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1215438.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13300..13905
                     /gene="recR"
                     /locus_tag="ACHU0J_000609"
     CDS             13300..13905
                     /gene="recR"
                     /locus_tag="ACHU0J_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1215439.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14015..15889
                     /gene="htpG"
                     /locus_tag="ACHU0J_000610"
     CDS             14015..15889
                     /gene="htpG"
                     /locus_tag="ACHU0J_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1215440.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16070..16714
                     /gene="adk"
                     /locus_tag="ACHU0J_000611"
     CDS             16070..16714
                     /gene="adk"
                     /locus_tag="ACHU0J_000611"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1215441.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16846..17808
                     /gene="hemH"
                     /locus_tag="ACHU0J_000612"
     CDS             16846..17808
                     /gene="hemH"
                     /locus_tag="ACHU0J_000612"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1215442.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17805..18764)
                     /gene="aes"
                     /locus_tag="ACHU0J_000613"
                     /pseudo
     CDS             complement(17805..18764)
                     /gene="aes"
                     /locus_tag="ACHU0J_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18916..20220
                     /gene="gsk"
                     /locus_tag="ACHU0J_000614"
     CDS             18916..20220
                     /gene="gsk"
                     /locus_tag="ACHU0J_000614"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1215443.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20353..22029)
                     /gene="ybaL"
                     /locus_tag="ACHU0J_000615"
     CDS             complement(20353..22029)
                     /gene="ybaL"
                     /locus_tag="ACHU0J_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1215444.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22267..23487)
                     /gene="fsr"
                     /locus_tag="ACHU0J_000616"
     CDS             complement(22267..23487)
                     /gene="fsr"
                     /locus_tag="ACHU0J_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1215445.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23705..25357
                     /gene="ushA"
                     /locus_tag="ACHU0J_000617"
     CDS             23705..25357
                     /gene="ushA"
                     /locus_tag="ACHU0J_000617"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1215446.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25394..25873)
                     /gene="ybaK"
                     /locus_tag="ACHU0J_000618"
     CDS             complement(25394..25873)
                     /gene="ybaK"
                     /locus_tag="ACHU0J_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1215447.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26077..26871)
                     /locus_tag="ACHU0J_000619"
     CDS             complement(26077..26871)
                     /locus_tag="ACHU0J_000619"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1215448.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27009..27350
                     /locus_tag="ACHU0J_000620"
     CDS             27009..27350
                     /locus_tag="ACHU0J_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1215449.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27565..30069)
                     /gene="copA"
                     /locus_tag="ACHU0J_000621"
     CDS             complement(27565..30069)
                     /gene="copA"
                     /locus_tag="ACHU0J_000621"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1215450.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30331..31263
                     /gene="glsA"
                     /locus_tag="ACHU0J_000622"
     CDS             30331..31263
                     /gene="glsA"
                     /locus_tag="ACHU0J_000622"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1215451.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31266..32558
                     /locus_tag="ACHU0J_000623"
     CDS             31266..32558
                     /locus_tag="ACHU0J_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1215452.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32589..32686
                     /locus_tag="ACHU0J_000624"
                     /pseudo
     CDS             32589..32686
                     /locus_tag="ACHU0J_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32683..33090
                     /gene="cueR"
                     /locus_tag="ACHU0J_000625"
     CDS             32683..33090
                     /gene="cueR"
                     /locus_tag="ACHU0J_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1215453.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33091..33549)
                     /locus_tag="ACHU0J_000626"
     CDS             complement(33091..33549)
                     /locus_tag="ACHU0J_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1215454.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33546..34463)
                     /locus_tag="ACHU0J_000627"
     CDS             complement(33546..34463)
                     /locus_tag="ACHU0J_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1215455.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34609..35286
                     /gene="fetA"
                     /locus_tag="ACHU0J_000628"
     CDS             34609..35286
                     /gene="fetA"
                     /locus_tag="ACHU0J_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1215456.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35273..36052
                     /gene="fetB"
                     /locus_tag="ACHU0J_000629"
     CDS             35273..36052
                     /gene="fetB"
                     /locus_tag="ACHU0J_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1215457.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36115..36969)
                     /gene="cnoX"
                     /locus_tag="ACHU0J_000630"
     CDS             complement(36115..36969)
                     /gene="cnoX"
                     /locus_tag="ACHU0J_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1215458.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37030..37839)
                     /gene="ybbO"
                     /locus_tag="ACHU0J_000631"
     CDS             complement(37030..37839)
                     /gene="ybbO"
                     /locus_tag="ACHU0J_000631"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1215459.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(37829..38451)
                     /gene="tesA"
                     /locus_tag="ACHU0J_000632"
                     /pseudo
     CDS             complement(37829..38451)
                     /gene="tesA"
                     /locus_tag="ACHU0J_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            38422..39108
                     /gene="ybbA"
                     /locus_tag="ACHU0J_000633"
     CDS             38422..39108
                     /gene="ybbA"
                     /locus_tag="ACHU0J_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1215460.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39105..41520
                     /gene="ybbP"
                     /locus_tag="ACHU0J_000634"
                     /pseudo
     CDS             39105..41520
                     /gene="ybbP"
                     /locus_tag="ACHU0J_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(41738..41893)
                     /gene="dinQ"
                     /locus_tag="ACHU0J_000635"
     CDS             complement(41738..41893)
                     /gene="dinQ"
                     /locus_tag="ACHU0J_000635"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1215461.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(42233..43327)
                     /gene="mnmH"
                     /locus_tag="ACHU0J_000636"
     CDS             complement(42233..43327)
                     /gene="mnmH"
                     /locus_tag="ACHU0J_000636"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1215462.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(43396..44322)
                     /gene="allS"
                     /locus_tag="ACHU0J_000637"
     CDS             complement(43396..44322)
                     /gene="allS"
                     /locus_tag="ACHU0J_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR1215463.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            44552..45034
                     /gene="allA"
                     /locus_tag="ACHU0J_000638"
     CDS             44552..45034
                     /gene="allA"
                     /locus_tag="ACHU0J_000638"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR1215464.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45112..45927
                     /gene="allR"
                     /locus_tag="ACHU0J_000639"
     CDS             45112..45927
                     /gene="allR"
                     /locus_tag="ACHU0J_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR1215465.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            46017..47798
                     /gene="gcl"
                     /locus_tag="ACHU0J_000640"
     CDS             46017..47798
                     /gene="gcl"
                     /locus_tag="ACHU0J_000640"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR1215466.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47811..>48083
                     /locus_tag="ACHU0J_000641"
     CDS             47811..>48083
                     /locus_tag="ACHU0J_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1215467.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        11443 a        12508 c        12793 g        11339 t
ORIGIN      
        1 aaattaacta acaatcagta ttttttatga attttatacg tggttaatac tggttttcgt
       61 atgagatcct gagttggtgg ttcaattact ccttaatgtt cgtaggttat gcataaaaag
      121 gccgcttacg cggccttagt gattacatgt tgtatcaatg atgatcgaca gtatggctgt
      181 gctcgatatc ttcattcttg cggctaaagc ggcggcgaac caccacaaag aataccggaa
      241 cgaagaagat tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg
      301 cgttctgcgc gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa
      361 acgccagcga ggtcatcagg atcggacgta aacgcatccg caccgcatca agcgtcgctt
      421 caatcagacc tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg
      481 cgttcttcgc cgacaaccca atggttgtga gcagtcctac ttggaagtaa acgtcattgg
      541 tcaggccacg gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca
      601 taacggagaa cggagtcgac cagctctcgt acagcgccgc cagacacagg aatacgacaa
      661 tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga gagacgttcc tgataggaca
      721 tccccgtcca gtcatagcca acaccggtag gcagtttgct cgccagttgt tccatcagct
      781 ccattgcttc accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca
      841 ggccgttgta acgttccaga cgcggcgaac cgtactccca acgagaagag gagaacgccg
      901 agaatggcac catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca
      961 gcatacggta tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa
     1021 agtcgttcac atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag
     1081 aaacacccag cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt
     1141 ccagaccgtt tggacgtacg ctggtcagca tatcagggtg cttcgctgct tctgcaagca
     1201 gctggttacg cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa
     1261 agtcaaagcc ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca
     1321 tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt
     1381 tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct
     1441 gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt
     1501 ctttggtcag atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg
     1561 cacctgctgg cagctgaacc atggtcataa atacgccctg gtcctcatct ggcaagaagg
     1621 agcttggcag acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacta
     1681 ggtaacgccc cgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct
     1741 tctcgaacat gcggttcaac cagccgaaga agcctttttt accttccccg tgatcgcctt
     1801 tggcaatcgg tttcagcatg gtggcacaaa gagccggagt caggatcaac gctaccagta
     1861 ccgacagcgc cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag
     1921 aaccgccaaa gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca
     1981 gagcgccctg aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt
     2041 ccgccataac acgctccacg ttttctacca caacgatagc gtcatccacc aacaagccga
     2101 tggcgagcac catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga
     2161 cggcaaaggt cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga
     2221 agttctgcag gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt
     2281 taaccacttc gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt
     2341 tcagacccga cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg
     2401 tatccagcgc gtttgcaccg gtcgccagct tgatccccag accggaagca ggttggccgt
     2461 taaactctgc gatgatgtcg tagttctcac cacccagctc aatcttcgcc acatcacgca
     2521 gcagtacgcg ggaaccatcc tgattcactt tcagcaggat tttgccgaac tcttcagtag
     2581 aggtcagacg cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg
     2641 taccaccgag ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat
     2701 caaccggcgt tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt
     2761 actgtgaacc gaacaactga acatcaccca cgcccgacgt acggctgatg gcatctttca
     2821 tattcgccgc tacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa
     2881 cgccgacaac catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa
     2941 cttcttgcgg cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga
     3001 tatccgcatc agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac
     3061 tgttagagga catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg
     3121 tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg
     3181 ccggcggtgc aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg
     3241 ccaacatgat gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag
     3301 gcatgtctta acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt
     3361 gcggcttgct ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc
     3421 actttctgca gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc
     3481 cacttatcgc caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg
     3541 ccaactacca gtacggtggc atcaccacgc ggcgtacggg ttacgccctg ttgcgggact
     3601 aaaatagcgt ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc
     3661 agagtgtgat ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca
     3721 acggtaacgt cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg
     3781 atcagcgaca ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt
     3841 ttcaggcgaa ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc
     3901 tgcacggttg ccagcacagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc
     3961 gacttaccaa tgcgaccgct aatcggagag gtgactttgg tgtaagccag attgatccgc
     4021 gcagtttcaa cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc
     4081 agagcctgat cgtacccttg cttactgatg tactgagtac cgagcagttt ctgataacga
     4141 ttcaccgtca attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca
     4201 ctgtcgtatg tcgcctgata ggtcgcagga tcaatctgat agagagagac accagcttcg
     4261 atgtcgctac cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact
     4321 tctgcgatcc ggtaggcact ggtgcgacct ggaagctcgg ttgtgatctg cagaggttca
     4381 gttttgactg ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg
     4441 tcgtcacatc ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta
     4501 aaccctctgt ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt
     4561 caaaagttaa taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc
     4621 acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac
     4681 aagaagcgca agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc
     4741 agggggtatc atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg
     4801 caatctactg gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag
     4861 aatccaatat tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct
     4921 cagtattaag agagatatta attcatgttc ttgaatccac ggtgacagaa gaacgacgtc
     4981 gattattgat ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc
     5041 aacaggcaca acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac
     5101 attgtattga agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta
     5161 tgcgcggcta tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc
     5221 ttaaaaaaga agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca
     5281 cgcttcgtaa tcctgccact aacgaataac cctgaatctg actccaggat ttttcctgga
     5341 cattttcgtc gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt
     5401 caggtcgttc ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa
     5461 cgatcacggc attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag
     5521 aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag
     5581 gcgcaacttg actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag
     5641 caggatctga cagatacatt agccaccctc gataaaatcg atcgcataaa agaagagaca
     5701 gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg
     5761 ttaacagcac ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagtacgctt
     5821 tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa
     5881 aacgatctgg cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg
     5941 caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg
     6001 actgatgtcg gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag
     6061 gcgttgctga atgcggagat tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg
     6121 caggatacct tgcaaaagca acgtgattac gtgacggcga acagcgcccg tctggagcac
     6181 cagttgcagc tgttgcaaga agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg
     6241 gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa
     6301 caggaactgg aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt
     6361 aatcagttga tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg
     6421 gaacgaaata ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc
     6481 ctttaccagc aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc
     6541 atcgcggatt tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag
     6601 agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt
     6661 cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa
     6721 cagttgggta accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg
     6781 agcgtatcga aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac
     6841 cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag
     6901 tcgatgaaaa tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc
     6961 gccggtttgc cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa
     7021 gcgtatcaac agaaactggc ttccgctgtg ggttccctgc gtaacgatag ccagctcaac
     7081 acaccaaaag cgatccttat cgacctgatc cgcgcgctgc cggtgtgcct gattattctc
     7141 gcggttggcc tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc
     7201 agtaaaaaac tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa
     7261 aatggcgttg ccgtgcgtca cttcggcatg ccggagcagc agaccagcca ctggcgtcgg
     7321 caaattgtcc gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa
     7381 ctttccccgc tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg
     7441 ctgctgattg ccttcctggt atggccaatg tgccgcgaaa gctggcgtga taaagagtcg
     7501 cacaccatgc gactagtcac cattaccgtg ctgtcgatta tcccgattgc attgatggtg
     7561 ctgactgcca cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc
     7621 gtttatctgg tgatcatctg gaacttgctc taccagacgg tattgcgcgg cttaagcgta
     7681 gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag
     7741 ggcgcagaag gtgccgaacc gccggaagaa cccaccattg cactggagca agttaaccag
     7801 cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca
     7861 atttggtccg atttgatcac cgtgttcagc tatctcgaca gcatcacgct ctggcattac
     7921 aacggcactg aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt
     7981 gcgattatcg cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttactggaa
     8041 gtgctggtgc tctcgcgact gaatatgcgc cagggcgcgt cgtatgccat tactaccatc
     8101 cttaactaca tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctct
     8161 tgggataaac tccagtggct ggccgcagca ttatccgtag gtcttggttt tggtttacaa
     8221 gaaattttcg gtaacttcgt ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt
     8281 ggcgatacgg taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg
     8341 acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc
     8401 gagcgtctga tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc
     8461 gtggcctatg gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag
     8521 cacccaaggg tgatgcacga accaatgccg gaagtcttct ttacggcatt tggtgccagc
     8581 acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag ccgtactgtc
     8641 gatgagctga accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt
     8701 aaccagcttg aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtaaaacgc
     8761 gactacaaag gcgacgatcc gacaccagcg gttgggtaat aaccaaaggg ggcgacattt
     8821 agttgccccg agattactga caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc
     8881 ttatccggcc tacgaaaccg caagaattca atatattgca ggagcggtgt aggcctgata
     8941 agcgtagcgc atcaggcagt tttgcgtttg cccgcaatct taggggacat ttagcggccc
     9001 catttatttc tcacttttcc gcctcatcat cgtgcgttaa tttcttttca taatcacgct
     9061 ttacaatatc cagcgcgcgc agcacggtac tggcagggat ctgattttcc tccagcagca
     9121 caatcaaatc gacagccagt ttgacatcgt caggggcatt ttcaagtgac atactctttc
     9181 cattgctagc gggttaagcg cgctagcctg ttttcgattt tttccagcgc atggcggcag
     9241 cgtgccaggc gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga
     9301 tcggtcaccc gcgccaggcg ggctctgcgt tcggcaacca tctcacgtag ccgccgctca
     9361 aaatcctgat gctgaatacg tttacgctgc cagcgggaaa ttttcggtgg cgcactgtcc
     9421 cactcgcgca atgaccaggc ggaggcttca cgcgcgatgg cttccagttg tgccgccaga
     9481 tgttccgcca gccaggccac ttgcggcagc tgttgctgct caactgcatg acgaagcgca
     9541 gccagattat cgcccgcctc gtcgagacaa gactgtagtg ttgtcgcacg agtctgaaaa
     9601 agatgcctgt cgaaacgagc acttagcgtg gcgaactgtg acaccggggc acaacgctga
     9661 cgcagcgtag cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga
     9721 cctcaatgaa aatgataatt gttatgctaa agtagccact atgtaagctg acaacactca
     9781 atatgcaacg aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg
     9841 gcgtggtatt accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg
     9901 cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc
     9961 tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc
    10021 ttattttgct cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca
    10081 tgttgctggt aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg
    10141 aaaagcaaga aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt
    10201 acattctggc gttttcgagc acaggcgcag gcggtcaaag gttaaacaac agttactttt
    10261 gatacgttta aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc
    10321 gactgcacag cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa
    10381 acccggcatt cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct
    10441 cagcatcgac ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac
    10501 cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt
    10561 accggtccgt aaaccgggca aactgccgcg tgaaaccatc agtgaaaatt acgacctgga
    10621 atacggcacc gatcagctgg aaatccacgt tgatgccatc aaaccgggcg acaaagttct
    10681 ggtggtggac gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg
    10741 tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc aacctgtttg atctcggcgg
    10801 cgaacagcgt ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca
    10861 ttaattatcg ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc
    10921 tcgccgttct gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt
    10981 cagagcctgc caatgagtta tcaggtttta gcccgaaaat ggcgcccaca aacctttgct
    11041 gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt
    11101 attcatcatg cttatctttt ttccggcacc cgtggcgtcg gaaaaacctc tatcgcccga
    11161 ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc
    11221 gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc
    11281 tcgcgcacca aagttgaaga tacccgcgac ctgctggata acgtccagta cgctccggcg
    11341 cgtggtcgtt tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt
    11401 aacgcactgt taaaaaccct tgaagagccg ccggagcacg ttaagtttct gctggcgacg
    11461 accgatccac agaaattgcc ggtgacgatt ttgtcacgct gtctgcaatt tcatctcaag
    11521 gcgctggatg tcgagcaaat tcgccatcag cttgagcaca tcctcaatga agaacatatc
    11581 gctcacgagc cgcgggcgct gcaattgttg gcgcgcgccg ctgaaggcag cctgcgagat
    11641 gccttaagtc tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggcg
    11701 gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt tgaagcgatg
    11761 gtcgagggca acggcgagcg cgtaatggca ctgattaatg aagccgctgc ccgtggtatc
    11821 gagtgggaag cgttgctggt ggaaatgctc ggcttgttgc atcgtattgc gatggtacaa
    11881 ctttcgcctg ctgcacttgg caacgacatg gccgccatcg agctgcggat gcgtgaactg
    11941 gcgcgcacca taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc
    12001 aaagaattac cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg
    12061 ctggcgttcc atccgcgtat gccgctgcct gagccagaag tgccacgcca gtcctttgca
    12121 cccgtcgcgc caacggcagt aatgacgcca accctggtgc cgccgcaatc agcgccgcag
    12181 caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc gcgccagcag
    12241 ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc cgctacccgc
    12301 gcgcggccgg tgaataacgc tgcgctggaa agactggctt cggtcaccga tcgcgttcag
    12361 gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc gtatcgctgg
    12421 aaggcgacca ctccggtgat gcagcaaaaa gaagtggtcg ccacgccgaa ggcgctgaaa
    12481 aaagcgctgg aacatgaaaa aacgccggaa ctggcggtga agctagcggc agaagccatt
    12541 gagcgcgacc cgtgggcggc acaggtgagc caactttcgc taccaaaact ggtcgaacag
    12601 gtggcgttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca tttgcgctcc
    12661 tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc gttgagcatg
    12721 ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc ggtgcgtacg
    12781 ccgctggagt ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt
    12841 attgcggata ataatattca gaccctgcgt cggtttttcg atgcggagct ggatgaagaa
    12901 agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa cgtgattgag
    12961 agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag cccagcagat
    13021 gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtta ccggcgaatc
    13081 tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc gcgtagagat
    13141 cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg ctgcagcatt
    13201 caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct ctgtatcctc
    13261 cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag cccgctgtta
    13321 acacagctta tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt
    13381 atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc gcaggcgctc
    13441 acccgggcga tgtcggaaat cggccactgc gccgattgcc gcactttcac cgaacaggaa
    13501 gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg tgtggtggag
    13561 agtccggcgg acatctacgc cattgagcag acggggcagt tttctggtcg ttattttgtg
    13621 ttgatggggc atctgtcacc gctggacggc atcggtccgg atgatatcgg gcttgatcgt
    13681 ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac caatcccacg
    13741 gttgaaggtg aagctaccgc taactacatt gccgagcttt gcgcgcaata tgacgtggaa
    13801 gccagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt cgatggcacc
    13861 acgctgtcac actccctcgc cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg
    13921 agcacctgct ctcgcttgaa attattctcc cttgtcccca tctctccctc atcctgtttt
    13981 taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt
    14041 tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt ccaataaaga
    14101 aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg
    14161 tgcgctatct aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt
    14221 cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga cccgcgacga
    14281 agtgattgac catctgggga ctatcgctaa atccggtacc aaatcattcc tcgaatccct
    14341 gggttctgac caagcgaaag acagccagct gatcggtcag tttggtgttg gtttctactc
    14401 tgcgtttatc gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga
    14461 aaacggcgtc ttctgggaat cggctggcga aggtgaatat accgttgccg atatcaccaa
    14521 agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga
    14581 tgactggcgc gtgcgttcca tcatcagcaa atactccgac catatcgcgc tgccggtaga
    14641 gatcgaaaaa cgcgaagaga aagatggcga aaccgttatc tcctgggcaa aaatcaacaa
    14701 agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt ataaagagtt
    14761 ctacaaacac atcgcccacg actttaacga tccgctgacc tggagccaca accgcgttga
    14821 aggtaagcag gagtacacca gcctgctgta catcccgtcc caggctccgt gggatatgtg
    14881 gaaccgtgat cataaacacg gcctgaaact gtacgttcaa cgcgtgttta tcatggacga
    14941 cgctgaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg actccagcga
    15001 tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg
    15061 caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga
    15121 aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc cggcggaaga
    15181 tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctactt ataccgattc
    15241 ttctgcgcag accgtgtctc tggaagacta cgtttcccgc atgaaagaag ggcaggagaa
    15301 aatctactac atcactgcag acagctatgc ggcagcgaag agcagcccgc acctggaact
    15361 gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg agtggatgat
    15421 gaactatctg actgagttcg acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc
    15481 gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga aagcactgac
    15541 tccgttcatc gaccgcgtga aagctctgct cggcgagcgc gtgaaagatg tccgtctgac
    15601 tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca
    15661 gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat acatcttcga
    15721 actgaacccg gatcacgtac tggtgaaacg tgcggcagat actgaagatg aagccaagtt
    15781 cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg gcacgctgga
    15841 agatccgaac ctgtttattc gtcgtatgaa tcagctgctg gtttcctgat gtcatgccgg
    15901 atgacctttg tgtcatccgg catttttctt ttcatcatct gtactttccg caaattatct
    15961 cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt tggtggtatc
    16021 gtttatcgct ttttcaaaaa aatcgacaca ttttaagggg attttcgcaa tgcgtatcat
    16081 tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca tggagaaata
    16141 tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat ctggctccga
    16201 gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg acgaactggt
    16261 gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgtaatggtt tcctgttgga
    16321 cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca tcaatgttga
    16381 ttacgttctg gaattcgacg taccggacga actgattgtt gatcgtatcg taggccgccg
    16441 cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga aagtagaagg
    16501 caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg aagaaaccgt
    16561 gcgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct actactccaa
    16621 agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac ggtaccaagc aggttgctga
    16681 agttcgcgct gatctggaaa aaatcctcgg ctaatcaagc cgcagcagat ctgctttcgg
    16741 gcaggtctgc ttatccacct caggcaatac ctctcacagc aattagttct tcttcctcac
    16801 ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg tcagactaaa
    16861 accggtatcc tgctggcaaa cctgggtacg cccgatgccc ccacacctga agcggtaaaa
    16921 cgctatctga aacaattttt aagcgacaga cgcgtggttg atacctcacg gttgttatgg
    16981 tggccgttgc tgcgcggcgt gattttgccg ctgcgctcgc cgcgtgtggc gaagctgtac
    17041 gcctctgtct ggatggaaga cggctcgccg ctgatggttt acagccgcca gcaacagcag
    17101 gcgttggcac aacgtttacc ggatacgcca gtggcgctgg gcatgagcta cggctcgcca
    17161 tcactggaaa gctccgtcga tgaactcctg gcagagcatg tagatcatat tgtggtgctg
    17221 ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg tatgggatga actggcacgc
    17281 attctggcgc gcaaacgtag cattccgggg atatcgttta ttcgtgatta cgccgataac
    17341 cacgattaca ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa acatggcgaa
    17401 ccggatctgc tgctgctctc ttatcatggc attccccagc gttatgcaga tgaaggcgat
    17461 gattacccgc aacgttgccg cacaacgact cgcgaactgg cttccgcact ggggatggta
    17521 ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc gggaaccctg gctgatgcct
    17581 tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat acaggtgatg
    17641 tgcccgggct ttgctgcgga ttgtctggag acgctggaag agattgccga gcaaaaccgt
    17701 gaggtcttcc tcggtgcggg cgggaaaaaa tatgaatata ttccagcgct taatgccacg
    17761 ccggaacata tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag ctgagcggta
    17821 aagaactgag cgccgtcgcg aagagcctcg tcggcggttt tcatcatccg tgaataatgc
    17881 aaaaaggcat gcagcgtacc tgggtagagt ttgaactcac agggctgctg atgcgccgct
    17941 aacgtctagt aaagcagacg gctgtcatcc agcagcggat cgaactccgc cccggcaata
    18001 aaacagggcg gaacttcgcg agtgagatca ttattaaaca gacagtaata cggtgactcg
    18061 cggtccgcgt cgttgcttaa atacgcctct tcatacatct gcaaatcctg ttgcgttaag
    18121 ccatcccaga caccgcccaa cagacgacga gtcacggaat cccgtaatcc gtaaagccca
    18181 taccacagca aaacacccgc aattttaccg caatcgatct gtttatcacg caaccacaac
    18241 gcactggcga gcgccagcat ggcacctgcg gaatcaccgg caaagccaat gcgggacata
    18301 ttgatttgat aatcctccgc ctgctggtgg aaataacaac aagcagccac aatttcctct
    18361 atcgcttgtg gaaaacgcgc ttcaggtgaa agggtgtaat taataccaat caccgtacat
    18421 tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt gccgagaata
    18481 aaaccgcctc catgcaaata aaatagcgtc gccgggctat ctggctgcgg acaaaagaga
    18541 cgtgtttcca cctgcccata ttttgttgga accatgtaag ctctggttgc catttctgga
    18601 gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc agcaatcgtt
    18661 cccgttgcgg gccaggacgg taaatccggc tgaagagtat tcacaacggt cttcatttca
    18721 gcagaaataa ggtccagaac aggtagtttg ttttccggct tcatacaaaa actcctttca
    18781 aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc tgatattgaa
    18841 actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct cagttaacca
    18901 cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaacatta cttccctgta
    18961 aatgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag cgccgcctgg
    19021 gtcgtaggta ttgaccagac gctggtcgat attgaagcga aagtggatga tgaattcatt
    19081 gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt agccgaagtg
    19141 ctttatcagg agctaaaaca gaaaaacctg attacccatc agtttgcggg tggcaccatt
    19201 ggtaacacca tgcacaacta ctcggtgctc gcggacgacc gttcggtgct gctgggcgtc
    19261 atgtgcagca atattgaaat tggcagttat gcctatcgtt acctgtgtaa cacttccagc
    19321 cgtaccgatc ttaactatct acaaggcgtg gatggtccga ttggtcgttg ctttacgctg
    19381 attggcgagt ccggggaacg tacctttgct atcagtccag gccacatgaa ccagctgcgg
    19441 gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg cactggttct cacctcatat
    19501 ctggtgcgtt gcaagccggg tgaacccatg ccggaagcaa ccatgaaagc cattgagtac
    19561 gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt cattgccgag
    19621 aatccgcagt ggtggcagca attcctcaaa gaccacgttt ctatccttgc gatgaacgaa
    19681 gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga caaggcgctg
    19741 gactgggtag agctggtgct gtgcaccgcc gggccaatcg gcttgtatat ggcgggcttt
    19801 accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggcgc tatagcggaa
    19861 ttcaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa tccgctgcgt
    19921 gtctattcgc acattgcgcc gtacatgggc gggccggaaa aaatcatgaa cactaatgga
    19981 gcgggggatg gcgcattggc agcgttgctg catgacatta ccgccaacag ctaccatcgt
    20041 agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc atcgttagcg
    20101 caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca ttcacctcgt
    20161 ttaacgcgcg gcttgccgga gcgtgaagac agccaggaag agtcttactg ggatcgttaa
    20221 gttatcgtcg gttcgtaggc cagataaggc gttcacgccg catctggcat ttggctctcg
    20281 atgcctgatg cgacgctggc gcgtcttatc atgcctacat atttttcata ttttacatcc
    20341 ggcaaccacc gtttaccccg tcaccacttc acccgccggt ggcgtttcca gcagttccag
    20401 catagtacgg gcaatttcac gttcacccat cactacctga ttcgcgccac gttcggtgat
    20461 atacgtcact tcatcgtcat aatgggcgcg ggcgataatc tcaatatccg ggtttttcgc
    20521 gcgggcagac gccacaatct cacccgcttc ataaccgttg gggatcgtca ggatcagcca
    20581 ttttgcacat tccagatgtg ccagttgcat aatttcttcg ttcgctgcat tgcccaatac
    20641 agcgcggacc ccacgctctc gcagctcatc aacacgggtt cgtgacgtct caatcaccac
    20701 cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac gaccgtaacc
    20761 caccaatagc gcatggttgc aaatatccac tgggatctgt ttctcttctt cgattgcctc
    20821 ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca gtagtgcgaa
    20881 cagtaccggg ttgagcataa tcgacaggat cgcccctgcc agtaccaggt tttgtccggc
    20941 ctgcggcagt aaattcaatg ccattcccag tcccgccagg ataaacgcga actcaccaat
    21001 ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa acagacggac
    21061 aaggaacagg gccgcgaccg atttaccaaa cagaataatt gccagcgtcg ccagcactgc
    21121 cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgactgaga caaaaaacag
    21181 caccgcaaac gcgtcgcgca atggcaacgt atcgtgggcg gcacggtgac tcaattcaga
    21241 ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat caaacagctc
    21301 taccgcacca aaggcaatcc ctaacgccag cgccagcact gacagggtaa acagctcgcg
    21361 agaaccggtt gccgcgctgc gtgccataat ccacggcacc agacggcgac ctaccagcat
    21421 cataatggcg ataaatgcga tcaccttgcc gatggtgatc cccatatcga ctgcaagagt
    21481 ggcaaagccc acatcgccct gttccatcat tcctgccact gcgggcagca acaccagcgt
    21541 cagaaccatc accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc cacgctgact
    21601 gtctattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg cggtggaaag
    21661 acataagccg aacacgatac cggtcattaa cgaccagccc agcacggcgg agagcgccat
    21721 acccagcagc gtcgccacgg ctatctgggc gatcgcgccg ggaatggcga tggactttac
    21781 cgccatcaaa tccttgagcg aaaaatgcaa accgacgccg aacatcagca aaataacgcc
    21841 cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag tgaatggtcc
    21901 tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt tattggccag
    21961 catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa gcggggtggc
    22021 gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggctg ggaaaaccaa
    22081 aattacaggt aatagtttat gacaatttca ttgatgatgt ttatgaataa ttgttgaatt
    22141 ttgcagaaaa atggaattag ctgcaaaaaa agcacggttc ggaaaacaga aggcggttta
    22201 acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca
    22261 atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg
    22321 caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga taagccccag
    22381 aactgccgct cccagacctc ccatgccgaa agcaaaaccg aaaaagagtc cagaaaccat
    22441 accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga atgccgaagc
    22501 gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggcggg cgtagggtaa
    22561 aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt tccgtccaat
    22621 tttatcccct acaggcccgc cgatcaccgt acctgccgca acggcaaaca ggaaggcaaa
    22681 cagatgaagc tgggcattct ggatagataa tccgaatttt tgcatcagat aaaaggtgta
    22741 atagctgctg atgctcgcca tatagaaata tttcgagaaa atgaggatta acagaatgct
    22801 gactgccagt accactttgt tgcgcggcag tggattgatt atcgtcgctt tgggttttcc
    22861 tttattcatt cggtgctgtg ccgagtacca acggctaatt tgcgccaaca ccacgatcgc
    22921 cagcagtgcc gcaagcacaa accaggcaac gttgcctttg ccataaggct cgataatcac
    22981 cgccgccagc aagggtccca gggaactgcc aaagttgccg ccgacctgaa agatagattg
    23041 cgccaggcca tgccgcccgc cggaagccat acgggctacg cgagaagatt ccggatgaaa
    23101 gaccgatgaa ccggtaccga ccagcgccgc tgccagcaga actgcgccaa aactgcccgc
    23161 cagcgcaagc agcaccagac cacttaaggt aaagcacatg ccaattggca acgaccacgg
    23221 catcggatat ttatcggtcc agtagccgac cactggttgc agtagcgaag aggcgagctg
    23281 gaaggtgagg gttatcatgc caatctgcat aaatgtcaga gaaaattctg actgaagcag
    23341 cggataaatc gccagaatca gcgattggat catgtcgttc agcagatgtg agaggctgat
    23401 agcacctaaa ataccaaacg atgttcgggc cttggtcgtt gacgcagccg cgcccgccac
    23461 aggctgggtt tgttcactca ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa
    23521 gaatgatctt atttgtgatt attacccgac taacatacct gtatgcgtcg tctgaaggaa
    23581 gtctcaacgc cgaatacaga atttctaatc tggatgcaga tttatcttca ccggacgcag
    23641 acttgtctat gatgttgcgt catactattt ttcaacacgt tgaaatcagg tcagggagag
    23701 aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg ttaaccacat ttacactggc
    23761 gagtgaaact gctctggcgt atgagcagga taaaacctac aaaattacag ttctgcatac
    23821 caatgatcat catgggcatt tttggcgcaa tgaatatggc gaatatggtc tggcggcgca
    23881 aaaaacgctg gtggatggta tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct
    23941 actttccggt ggcgacatta acactggcgt gcccgagtct gacttacagg atgccgaacc
    24001 tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta atcatgaatt
    24061 tgataatccg ctcaccgtat tacgccagca ggaaaagtgg gccaagttcc cgttgctttc
    24121 cgcgaatatc taccagaaaa gtactggcga gcgcctgttt aaaccgtggg cgctgtttaa
    24181 gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc gatgacacag caaaaattgg
    24241 taacctggaa tacttcactg atatcgaatt tcgtaagccc gccgatgaag cgaagctggt
    24301 gattcaggag ctgcaacaga cagaaaagcc agacattatt atcgcggcga cccatatggg
    24361 gcattacgat aatggtgagc acggctctaa cgcaccgggc gatgtggaga tggcacgcgc
    24421 gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc cggtctgcat
    24481 ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt acgccatgca aaccagatca
    24541 acaaaacggc atctggattg tgcaggcgca tgagtggggc aaatacgtgg gacgggctga
    24601 ttttgagttt cgtaatggcg aaatgaaaat ggttaactac cagctgattc cggtgaacct
    24661 gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc gtgctttaca ctcctgaaat
    24721 cgctgaaaac cagcaaatga tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca
    24781 gctggaagtg aaaataggca aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtgcg
    24841 ttttgtacag accaatatgg ggcggttgat tctggcagcc caaatggatc gcactggtgc
    24901 cgactttgcg gtgatgagcg gaggcggaat tcgtgattct atcgaagcag gcgatatcag
    24961 ctataaaaac gtgctgaaag tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac
    25021 cggtaaagag gtgattgatt acctgaccgc cgtcgcgcag atgaagccag attcaggtgc
    25081 ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac ggcaaactga acgaccttaa
    25141 aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt atggcgacat taaacttcaa
    25201 tgccaccggc ggtgatggat atccgcgcct tgataacaaa ccgggctatg tgaataccgg
    25261 ctttattgat gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc tggatgtgag
    25321 tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg gatgtttgca
    25381 tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa tttggcatcg aggatctttg
    25441 ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt gccgccggaa acataaatag
    25501 tggcaaattc ttgtgcgggg gcgtcgataa tcgttggcag acgttttttc tgccccagtg
    25561 ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc gaccatcgga tcggccatct
    25621 caactttctt ggcacccagc gcttttgcta cttttttaag atccagttga cctgcgaccg
    25681 gcgtaacggc cacggcaagg tgtttcatat caccgttcac tgccaccagc agcgttttgt
    25741 agacctgatc cggattcaaa cctaattttt tgacgacttc atcgccaaaa ttggtttcag
    25801 ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt gtttttttcg agtaatttaa
    25861 ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag catacgcctg
    25921 atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg gtgagattga
    25981 gcgacaatcg agttacaccg tcgcttaaag tgacggcata ataataaaaa aatgaaattc
    26041 ctctttgacg ggccaatagc gatattggcc atttttttag cgcaacattt gcggcaaatt
    26101 cccttctcca tacaggtgta gtgcaccgac cgcgaccaca tatcgccccg gcggcatggc
    26161 gcgtaattta tcccgccagg cgagatttcg ctgatgcatc agcacatcgt acagcgactg
    26221 actgaacgta ttgggcagcg ttatatcatt attttgcggc ggtgcattca gccaccagct
    26281 catcatttgt tgcagcaacc gtgcgttggt atgccagtgg gtcagcgtat cgtccagcag
    26341 cgccagtcct ttgtcaggga gctggagcaa catggcaatc tggttttcag ccccttccag
    26401 ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat agctggtaat cgataccgta
    26461 ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga agaaccatcg cgatttgcca
    26521 cagcggttgg gtagaaaaga gtgagggaga aatgcccatc tcctggctaa tgtgctgcag
    26581 gttttggagt tgctcctcgc taatgcgctc ttccagcgcc tcgcaggcag gcaaattagc
    26641 aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg cgtttttgag
    26701 ctttttgagc aaacgggtgg gcaggggagc catatcgtgg ctacccatat gaatactgcc
    26761 aatcagatga aaatggcgat tgccggggag ggtgatatcg atggcaggcc aggtgtaatg
    26821 attaccgcgc agcgcggccc aaagcgtttt tacccggtac aacagatcca tacgacctcc
    26881 ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg caaatggagt tggggacttg
    26941 tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct attcacaggg aagcaacagt
    27001 taaaaaccat gaaacaggca acaagaaaac cgacgacacc cggagatatt cttctctatg
    27061 aatatctgga accgctcgat ttgaaaatca atgagttagc agagttgctg catgttcatc
    27121 gtaatcgcgt cagtgcactg atcaataaca atcgtaaact caccactgag atggcattcc
    27181 gtctggcgaa agtttttgat accacagtcg attttaggct aaacctccag gcggcagttg
    27241 atctttggga agttgaaaac aacatgcgca cccaggaaga attgggacgg attgaaacag
    27301 tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa ggtcgcgtaa ggccaaaaca
    27361 tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca tccggcatcc gagcaccatt
    27421 gcctgatgcg acgcttacgc gtcttatcag gcctacaaac ctgtgcctga accgtaggcc
    27481 ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgc gccgccctaa
    27541 agcagcgcat ccgcaatgac gtacttattc cttcggttta aaccgcagca accggttggc
    27601 gttacttact acggtaattg acgagagcgc cattgccgct ccggcaacta ccgggttaag
    27661 cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tgctgttgta
    27721 gataaaggca ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatcgcgag
    27781 cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc
    27841 aacatcactg ccgccaccca tcgcaatgcc gacatccgcc tgggccagcg ctggcgcgtc
    27901 gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcagac gtttgatcgc
    27961 ttcggcttta ccgtccggca gcactccggc aatcacttca tcaattccag cttctttggc
    28021 gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt
    28081 atgcaggcgt tgcagcgccg ccacgctatc actacgcaac ggatcgcgta cagccagcag
    28141 ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt gctccttgcg atgcctgagc
    28201 tgaaatatcg gcttcaatcg ctttagtatc gacttgctga tcatttaaca acgcctgatt
    28261 acccagcaat aacgcatgac cttctgcttc accgctcacg cccagcccgc gcaatgtgcg
    28321 gaaaccgttg acctgcggta gctgcatatc gcctgcttta tcaaggatcg ctcgtgccaa
    28381 tggatggctg gaaccttgct ccagcgccgc cgccagacgc agcgcctgcg cttcgtcaac
    28441 atcagcaaat gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt
    28501 atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag
    28561 cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagtcc
    28621 cagcgcacac gggcaggcga taatcagcac cgtagtggca atcaccaggg tatagacaat
    28681 ctgcggtgcc ggaccaaaga aataccagat tgccgcactg acaagcgcaa taaccactac
    28741 taccggcaca aatatggctg agattttatc cgccagctga ccgatttctg gcttgctgct
    28801 ctgggcctgg cgcaccatgc gaatgatcct tgatagcgtg gtatggctgc caaccgcact
    28861 ggcacgaaac agcacactgc cgtcctgtac cactgtcccg gcatggacgc tatcgccttc
    28921 gcctttttgc tgcgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc
    28981 ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gcaacatacc
    29041 tggctgcact tctgccagag gcacgctttt ttcaccttcg tcagtaacca ggcgcgccgt
    29101 cggtggggtt aaatcgagta atttctccag tgccttagaa gaacgctgac gtgcgcgtgc
    29161 ttccagcata tggccgagat tgatcagacc gataatcatc gcactggctt catagtaaag
    29221 atgtcgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca
    29281 cgccacgccc gttcccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct
    29341 tttccatgca ctgcggtaaa aatggccgct ggcgaaaacc atcaccgcca gggttatcag
    29401 gccgataacc aaccacaggc tgcggttgtc ggcggtgact atcatgttat cgccgatcat
    29461 cccccagacc atcaccggga tacccaccgc cagtgcgaca attgcctgcc agcggaagcg
    29521 cttcatcgta gcgacggcgg tttcttgctg gcgttcgcgg cgtttcgcgt catcttcaat
    29581 cgcttccgcg ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact
    29641 gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac
    29701 gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc cgctcagcag
    29761 caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt
    29821 cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga
    29881 tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt
    29941 aacgtgcgct tcagtgatag acacatccgc ttgttccaca tccgggcgct gttcaagact
    30001 ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccaggttca ggtcgatagt
    30061 ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt
    30121 ccagcaaggg gaaggtcaag aaattaataa accaggcggg taaaagtccg taaagattaa
    30181 aaaatcggct cgatttgcat caggattaga catttatttc tttgttttcc tgtagttaag
    30241 ttgcgggtgc taagttaaat caggatgcct gaaaatcggc actggggtga ggaattacct
    30301 cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca
    30361 gtggatcagg cttacaccca atttcactca cttaacggcg gacaaaatgc cgattacatt
    30421 ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg ctatcgtgac ctgcgatggc
    30481 aacgtctata gtgcgggtga cagtgattac cgctttgcac tggaatccat ctctaaagtc
    30541 tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg cggtacagga caaaattggc
    30601 gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa
    30661 ccgctttcgc cactggtaaa tgctggcgct attgccacca ccagcctgat taacgctgaa
    30721 aacgccgaac aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag
    30781 gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taatcgggcc
    30841 attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac
    30901 gtgtacaccc gtcagtgctc tacgctcatc aatactgttg aactggcaac gcttggcgcg
    30961 acgctggcgg cgggtggtgt gaatccgttg acgcataaac gcgttcttca ggccgacaac
    31021 gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg
    31081 gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc
    31141 cctggcgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caatagtgtt
    31201 cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc
    31261 tgatcatgat gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaacgtcg
    31321 tttccatcgg tattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg
    31381 cgttgctaat ggaagcctcg acctggggcg cctttgcttt tggcggtatt gtggctatgt
    31441 tttccggtta tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggtattatcg
    31501 acttttttcg tcgcggatta ggcaacggcg tcttttcgct agcgctctcg ttgctatacc
    31561 tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc ttttggcgct tatgccgtgc
    31621 agtttttgca tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca
    31681 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
    31741 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc
    31801 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
    31861 ttgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
    31921 tgaaagatcc gcaggtcatt atgccacggg cgtttctggt ggcgattggc gttaccacgt
    31981 tgctttatat ctcgctggcg ctggttttgc ttagcgatgt atcggcatta gagttagaaa
    32041 aatatgctga taccgccgta gcgcaggctg cttctccgct gctcgggcat gtgggttatg
    32101 tgatcgtcgt catcggcgct ttactggcga cggcttcagg cattaacgcg aacctgttcg
    32161 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
    32221 aatccctgtg gcggcagagt acctggggca atatcatcgt cgtggtgttg ataatgctga
    32281 tgacggcggc gctaaattta gactcactcg ccagcgttgc cagcgccacc tttttgatct
    32341 gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
    32401 cgattcttat cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagcc
    32461 tgtggtccca gggcagccgt gcgttgatat ggattattgg tgcaatctta ctcagcctta
    32521 ttgtggcaat ggtcatgaag cgtaataaaa ccgtataaca cctctctgtg cgcagtactt
    32581 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
    32641 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta
    32701 gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg
    32761 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
    32821 ctgaccttac tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg
    32881 gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag
    32941 aaggtggcgg agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg
    33001 gcactggcga atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc
    33061 tccggctgct gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt
    33121 tatcccttct atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct
    33181 gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc
    33241 cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg
    33301 cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt
    33361 caggatggca aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag
    33421 gccggtaatc accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc
    33481 ggctgccagc agcaacccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa
    33541 ctccatcatg gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca
    33601 atcgacccca tcaggctgct ggcctctaat ggcatcatca ctactttgct gttactggag
    33661 gaaccgatct gctgtaacgc ttcggtgtat ttttgcgcta cgaagtagtt caccgcctga
    33721 atatcaccgg aggcgatagc ttcagacacc attttggtgg cgcgggcttc tgcttcggcg
    33781 gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg
    33841 atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct
    33901 tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata
    33961 agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg
    34021 ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc
    34081 atttcgtcaa gttccattga acccaacacg gtacggatgt tagtcatggt caggttgatg
    34141 atcgccagct ccagattgct gacttcatag gcagcgcgtg gcgcgtcaat cacctgaata
    34201 aaacagactg cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg
    34261 atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc
    34321 accagactga gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat
    34381 tgatagccct gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg
    34441 agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa
    34501 aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc
    34561 tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat
    34621 agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatacgaa gattcttaat
    34681 aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt
    34741 ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta
    34801 ctgtttgaag gtgaggatgt cagcacacta aaaccagaaa tctaccgcca gcaagtctct
    34861 tactgcgccc agacgccgac gctgtttggc gatacggtat acgataatct gatctttccc
    34921 tggcagatcc gtgaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc
    34981 gctttgccgg acagcatttt gacgaagaat atcgccgagc tatctggtgg tgaaaaacaa
    35041 cgcatctcat tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata
    35101 accagtgcgc tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg
    35161 cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg
    35221 gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc
    35281 gcataatatt actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt
    35341 aattagccat aaagaaaaac tggcgctgga gaaagatatt ctatggagcg tcgggcgagc
    35401 gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc
    35461 cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa
    35521 acgcagtaaa tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc
    35581 gggaattacc ctggcggtgc tgattctctc aggggcgatt gaatttatcc cgatgcaggt
    35641 gatccctgtc gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa
    35701 caatttaggg caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg
    35761 cgcgacgccg aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat
    35821 tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg
    35881 gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt
    35941 tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa
    36001 gttttataat tcgcgccacc agttggtggt gactcaattg aagaagaaat gatgcgatgc
    36061 cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgaacaacgg aatatcaata
    36121 caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc
    36181 agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg
    36241 cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctgat gcagttgcag
    36301 cgccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg
    36361 ttgaatttcc ggcttatcag ccgcctgctt cagcagttcg atttgcgcta ccagcccctg
    36421 gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga
    36481 acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga
    36541 caactgccag gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag
    36601 ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc
    36661 gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa
    36721 cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc
    36781 gcagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat
    36841 cggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt
    36901 ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc
    36961 tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg
    37021 cgcttcaact cacccctgca atattttgtc catcacgcgc ccaggcagca ggcgctcgag
    37081 caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat
    37141 aaaagcatgg cgcactttgt cgaccaccgc ttccggtccc aacgtaaagc gggcggcgat
    37201 gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt
    37261 acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg
    37321 cagcgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg
    37381 cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa
    37441 catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg
    37501 ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt
    37561 gttaaagatc ccatacagac aattatcggt cagggcgatc acctcgtctg ctgcgcgatt
    37621 aacactttct ggtgaatcca gatcgatcaa cacgccggta aatcccatgt tgttcatacg
    37681 ctcaacatca ttcggttttc ggcaacctgc cagcacatga aaaccctggc gttttaattc
    37741 gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat
    37801 aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact
    37861 gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat
    37921 cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat
    37981 caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat
    38041 agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct
    38101 gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccatcat
    38161 tgcgcccagt tcaaccagca cccaacgcgg ctgatgctgt ttgagcagag ccggaaggcg
    38221 cgccagccct tgttgcgagg tgtcgccgct gatgctggca ttaactaccg acgttttact
    38281 ctgccactta tcattcaaca aggcaggcca ggccgcgctg gcagacattc gatacccggc
    38341 gctcaggcta tcacccagaa tcaataacgt gtccgctgcg gcggcacgga aggttaacag
    38401 gaccaggaac aggaagggca aatgccagcg gaaaacattg ttgaagttca tcatcttaag
    38461 aagtccgtcg gtcaggggga gcatgaactc tccatcctca ccggagttga gctggttgtc
    38521 aaacgtggcg agaccatcgc actggtgggc gagtcgggat cgggtaagtc aaccttgctg
    38581 gcgatcctcg ccgggcttga tgacggcagc agtggcgaag tgagtctggt gggacaaccg
    38641 ctacataata tggacgaaga agcgcgggca aagttgcgcg cgaagcacgt cggctttgtt
    38701 tttcagttat ttatgttaat tcctaccctt aacgcgctgg aaaacgtcga gcttccggct
    38761 ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg cgaaagcgtt gctcgaacag
    38821 ttagggctgg gtaaacgtct ggatcatctt ccggcacagc tttccggcgg tgaacagcaa
    38881 cgagtggcgc tggcacgagc ctttaatggt cgacctgatg tgctgtttgc cgacgaaccc
    38941 accggcaacc ttgaccgcca gacgggcgat aaaattgccg acctgctgtt ttccctcaac
    39001 cgtgaacatg gcaccacgtt gattatggtg acccacgacc tgcaactggc ggcacgctgc
    39061 gaccgctgct tacggctggt gaacgggcag ttgcaggagg aagcatgatt gcacgttggt
    39121 tctggcgcga atggcgttcg ccgtcgctat taattgtctg gctggcgcta agcctggcgg
    39181 tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat ggagaagggc ttaagccagc
    39241 aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag ttcacgcgaa gtgccgcaag
    39301 cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg caagcagctg actttcgcca
    39361 caatgacctt tgcaggcgac acaccgcagc tggcgaacgt caaagcggtg gatgatatct
    39421 acccgatgta tggcggtctg caaactaatc cccctggcct gaaaccgcag gcgggcagcg
    39481 tattgctggc cccacgcctg atggcactgc ttaacctgaa aacgggcgac accattgacg
    39541 tgggcgatgc caccttgcgg attgccggag aagtgattca ggaaccggat tccggtttta
    39601 accccttcca gatggctccg cgtctgatga tgaatctggc ggatgtcgat aaaaccggag
    39661 ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt cggcggcaac gagaaccagc
    39721 tcgacggcta tgagaaatgg ttgttacctc agcttaaacc cgaacaacgc tggtacggtc
    39781 tggaacagga cgaaggcgcg ctggggcgat cgatggaacg ctcgcaacag ttcctgctgc
    39841 tttcggcgct tctgaccttg ctgctggcag tggcagcggt ggcggtagcg atgaatcatt
    39901 actgtcgcag tcgctacgat ctggtggcga tcctcaaaac gctgggggca gggcgagcgc
    39961 aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct gacgctttca gccgttaccg
    40021 gtggggccat aggcctgttg ttcgaaaacg tgttgatggt gctgctcaag cccgttctgc
    40081 ctgctgcact accgccagcc agcctctggc cgtggctgtg ggcgcttggc accatgacgg
    40141 tcatctcgct gctggtgggg ctacgaccat accgcttgtt gctggcaacg cagcctttac
    40201 gcgtattacg taatgatgtg gtcgcgaacg tctggccgct gaagttttat ctgccgattg
    40261 tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg tggcagcatg ctgctttggg
    40321 cggtgctggc gggcgcggta gtactggctt tgctgtgcgg tgtgctgggc tggatgctgc
    40381 tgaatgtact tcgccgcatg acgctgaaat cgctgcctct gcgcctggcg gttagccgcc
    40441 tgttacgtca gccgtggtca acgttaagcc agctttcggc attttcgctc tcctttatgc
    40501 tgctggcact gctgctggtg ctgcgtggcg atctgctcga ccgctggcag cagcagctac
    40561 cgccagaaag cccgaactac tttttaatta acatcgccac agaacaggta gcaccgctaa
    40621 aagcgttcct cgcggaacat catatagtcc cggaatcatt ttatccggta gtgcgggcgc
    40681 ggctgacggc gattaatgat aagccgacag aaggtaatga agatgaggcg cttaatcgcg
    40741 aactcaatct tacctggcaa aatacgcggc ccgatcataa tccgattgtc gccggtaact
    40801 ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct ggcgaaacgc ttaaacgttg
    40861 ccctcggtga taccgtgact tttatgggcg atacccagga gttccgcgct aaagtgacca
    40921 gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt ctattttatt ttccctgaag
    40981 gggcattaga cgggcaaccg cagagctggc ttaccagttt ccgctgggag aatggcaacg
    41041 gcatgttgac gcaactcaac cgccagttcc cgaccattag cctgttagac attggcgcga
    41101 ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg ggcgctggag gtgatggtgg
    41161 tgctggtcac cgcctgcggt atgttgctgt tgctggcaca ggtgcaggtg ggaatgcgcc
    41221 agcgtcatca ggagctggtg gtatggcgca cactcggtgc ggggaaaaaa ctgctgcgca
    41281 cgacattgtg gtgtgatgtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt
    41341 ggtgcggaaa cggcactggc agtgttgcag gcgaaagtgt ttgatttccc gtgggagcca
    41401 gactggcgat tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc
    41461 ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgtggggtga
    41521 tgacaggtgt tagattgcct gatgcgctac gcttatcagg cctacgaaga gcgtgcaata
    41581 tattgatttt gcaagattgt gtaggccgga taaggcgttt acgccgcatc tggcatgaac
    41641 agtgtgcatt ttggcaataa atatgaaacc aactgtcatg tcggtttttt taaaggccga
    41701 aggaggtaaa ccttcggcct tagctacgtt tcctcagtta ttcaataaac tgacggatca
    41761 gttccagcag cgcgattagc gccttaagaa cgatgagaac tttatcgaga aaagttttca
    41821 tccgttgctc ccttaaggga gaaagacaat cacagcttac ccttacgttg cctataacta
    41881 tgattttctt catgtgctag atgaggactg tctctctcca aattagagca ccagaatcag
    41941 caccacctgt atcctgacca ggacttaaaa gtacttttcc gcactgcggt aatcccttga
    42001 ggcagtatca ctactagcgt cgggattgta tcgccaacat agctgtttga atatgtaggc
    42061 atcctgactt ataattcact tgctagatga ggactgttta atttctgccc ggaaccacca
    42121 agtcttgggc ggaaaaaata aaaaaaccga ctgtcatgtc ggttttttta tatctgtatt
    42181 ctggtgcaac cattttgttc tcacgacaat cgccgtttca gaataccgcc catcactgcg
    42241 ccttaaccca taccgccact tccgcccact caccgcgaaa gacaactttt tccgcttttt
    42301 tctcaagctg atagcgatac attgggtcgt aatattcttt aagtaacggc accagccagg
    42361 ccagatgacc gtcggtgctg ccggtggtga gttgcgttgt cagcgccgta tccagctggg
    42421 cagcgagttc gttatagcgc tgtaacccca gccgacgctt aatcgccgaa agtccgtgat
    42481 gcaggtattc gcaatattcc tgccagccct gttcgtcgcc ataagcgtgg gtaaaatcat
    42541 gatgcatacg caagaaatac tcttcattca ggcgttcaag acggatctca aacggatctt
    42601 ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag acattccggc aggtgattcg
    42661 aaccgatcat ccggctttca tcttccagca cccacaggcg caaatcctga cgggcgtcgg
    42721 tttttagcat ttcggcagcc agcaggtttt caaaactcgc ctggctaagt tgtggttgta
    42781 acgtgcgacc aaacgccgaa ccgcgatgac gcgccagccc ttccagatca acaccgtttg
    42841 gctgttgttg cactaatagc gttttaccgc tgccggtaca accgccaatc agcactatcg
    42901 gtttttgtgc cagttcaatg gtcgcctgaa tcgcggtctg gcgcagtgcc ttataaccgc
    42961 cttccaccag cggataatca atccccgctt catgcagcca gcgttgcaca atatgtgaac
    43021 gttgaccgcc acgggcgcag cagagaatac cttgcggatt ttgcaggcac gctgcccgcc
    43081 aggcgtacat acgctgctga cgaatttcac ccgccaccag tttatgcccc agcgccagcg
    43141 ctgcgtctga gccttgctgt ttatagcagg tgccaacgga ggcgcgttca tcgttatcca
    43201 ttaacggcag attgatagcg gcgggcattg cgccttgctc aaactcgata ggggcgcgaa
    43261 catcaataat gggcgtatca gcaatcagca gggcacgata gtcctgttcc gtgtgtctct
    43321 cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc tattttacgc gccaacgcgc
    43381 aaggaaactt gatttttaac tgcgtggggt gccgaaaatt tctaaaaatc cgctgatttc
    43441 cggcctgtgt tgggtaaaca aggtcacaat atcttctacc gctttgcagc tgccaaattt
    43501 gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt gggattaccc ggctgaccag
    43561 ttgttgatta tcgatcattg actggcaaag tgattttggc aaaaaaccaa tgccaacacc
    43621 cgccagatgg gcggcgattt tcgtttccat atcgggaaca ataatctctt tttgccccgg
    43681 caatcgccag gcgacgcgtt tggttaaggt gccggcgctg tcttcaatat tgaccgccgg
    43741 aaagcgacgc aactgcgctt ctgttagcgg ctcttcaacg ttcgccagcg gatgatccgc
    43801 cgccatgaca aatcgccatt gcaccgatcc taagggatca agactaaagg tatttgccag
    43861 cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct tcgtacaata gcgagtccca
    43921 gacgcccata tagatttgtc gggagatgtg aaactgggta aaggggtaac gctcattcag
    43981 ccacgccagc aactgggcga cggcctgggg gttatagagc aggttgttga tgacaatatt
    44041 cacctggcgt tccacgccat cattcacctg ttgcagctcg ctgggcatac tttccagcca
    44101 gctcagccag tctctggcct gggaaagtag atgctcgcca gccgctgtca acgttacgct
    44161 acgagtcgta cggaaaaaca gcgctactcc ggtattctct tccagaagtt taatgcgata
    44221 actgatcgtc gccgtggttt tacataatcg ttctgccgct tttgaaaaac ttcctgtttc
    44281 agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac atcttcaggt atcccctttt
    44341 aaatccgcaa gttgcgtgat tttcttatcc tctgatttat cagtattttt acatgataat
    44401 cctgttcaat ttgtggacta aatctagttt tggaaaaaca ttccaacttt tgtattaatg
    44461 ttgttctctt aaggttttag attgcctgtt attgaaacca agctgaccgg tcggcggtgg
    44521 ttgaacggaa ttatgttaca aggacaaaaa gatgaaactt caggtattac cgttaagtca
    44581 ggaagccttt agtgcttatg gcgacgtaat cgaaacgcag caacgggatt ttttccatat
    44641 taacaatggc ctggtggagc gttaccacga tttggcgctg gttgagattc ttgagcaaga
    44701 ccgtacgctt atcagcatta accgcgcgca accggcgaat ctgccgctga ccattcacga
    44761 actcgaacgt catccgctgg gcattcaggc ctttatccca atgaaaggtg aagtgtttgt
    44821 ggtggtcgtg gcgttaggcg acgacaaacc agacctgtca acgctgcggg cgtttatcac
    44881 caacggtgaa cagggggtga attaccatcg taacgtctgg catcacccac ttttcgcctg
    44941 gcagcgcgtc accgattttc tgaccatcga tcgcggcggc agtgacaact gtgatgttga
    45001 aagtattcct gaacaggaac tctgttttgc gtgacgcctg caaccgactt gcataagata
    45061 aactaattgt tcattgttta tgctcacttg taggtcggag ttaacgtagg tatgacggaa
    45121 gttagacggc gcggcaggcc aggacaggcg gagcctgtgg cacagaaggg cgcacaggcg
    45181 ttagagcggg gaattgcgat tctgcaatat ctggaaaaaa gtgggggaag ttcgtcggtt
    45241 agcgatattt ctctcaatct ggatttgccg ctctccacga cctttcgctt gctgaaggtt
    45301 ttacaagcag cggattttgt ctatcaggac agtcaattag gctggtggca tataggatta
    45361 ggtgtcttta acgtcggtgc ggcgtacatc cataaccgcg atgtcctctc cgtcgccggg
    45421 ccgtttatgc gccgcctgat gttactttcc ggcgaaacgg tcaatgtcgc gatccgtaac
    45481 ggcaatgaag cggtattaat tggtcagtta gagtgtaaat cgatggtcag gatgtgtgcg
    45541 ccactgggca gtcgtctgcc gctgcatgct tccggtgcgg gcaaagcgct gctttatccg
    45601 ctggcggaag aggagttgat gagcatcatt ctgcaaaccg gtttgcagca gtttacgcca
    45661 actacgcttg tggatatgcc caccttgctg aaggacctgg aacaagcgcg tgaactgggc
    45721 tataccgtag ataaagaaga gcatgttgta ggtctgaatt gcatagcttc agcaatttac
    45781 gatgatgtcg gtagagttgt tgccgctatc tccatctccg ggccttcatc aagactgaca
    45841 gaagatcgtt ttgtcagtca gggtgagctg gtcagagaca ccgcccgcga tatcagcacg
    45901 gcgttgggac tgaaagcaca tccatagtgt ctgtcgcatc ccgctctgcg gagcgggttt
    45961 ttttgacaaa atttgaaagt tggaaaattt ttccaactaa tagaggtagg aataaaatgg
    46021 caaaaatgag agccgttgac gcggcaatgt atgtgctgga gaaagaaggt atcaccaccg
    46081 ccttcggcgt tccgggagct gcaattaatc cgttctactc agcgatgcgt aagcacggcg
    46141 gtattcgtca cattctggcg cgtcatgtgg aaggtgtttc gcacatggcg gaaggttata
    46201 cccgcgcaac ggcagggaat atcggcgtat gtctggggac ttccggtcct gcgggcacgg
    46261 acatgatcac cgcgctctat tccgcttctg ctgattccat tcctattctg tgcattaccg
    46321 gccaggcacc gcgcgcccgt ctgcataaag aagattttca ggccgtagat attgaagcaa
    46381 ttgctaaacc ggtcagcaaa atggcggtta cagttcgtga agcggcgctg gtgcctcgcg
    46441 tgctgcaaca ggcatttcac ctgatgcgtt ctggtcgtcc gggtccggta ctggtggatt
    46501 taccgttcga cgttcaggtt gcggaaatcg agtttgatcc tgacatgtac gaaccgctgc
    46561 cggtctacaa acctgctgcc agccgtatgc agatcgaaaa agctgtagaa atgttaatcc
    46621 aggccgaacg tccggtgatt gttgccgggg gcggggtaat caatgctgac gcagctgcac
    46681 tgttacaaca gtttgctgaa ctgaccagcg ttccggtgat cccaacgctg atgggctggg
    46741 gctgtatccc ggacgatcat gaactgatgg ccgggatggt cggtctgcaa accgcgcatc
    46801 gttacggtaa cgcaacgttg ctggcgtccg acatggtgtt tggtatcggt aaccgttttg
    46861 ctaaccgtca taccggttcg gtagagaaat acaccgaagg gcgcaaaatc gttcatattg
    46921 atattgagcc gacgcaaatt ggtcgcgtgc tgtgtccgga tctcggcatt gtctctgatg
    46981 ctaaagcggc gctgacactg ctggttgaag tggcgcagga gatgcaaaaa gcgggtcgtc
    47041 tgccgtgtcg taaagaatgg gtcgccgact gccagcagcg taaacgcact ttgctgcgca
    47101 aaacccactt cgacaacgtg ccggtgaaac cgcagcgcgt gtatgaagag atgaacaaag
    47161 cctttggtcg cgatgtttgt tatgtcacca ctattggtct gtcacaaatc gctgcggcac
    47221 aaatgctgca tgtctttaaa gaccgccact ggatcaactg tggtcaggct ggtccgttag
    47281 gctggacgat tccggcggcg ctggggggtt gtgccgctga tccgaaacgc aatgtggtgg
    47341 cgatttctgg cgactttgac ttccagttcc tgattgaaga gttagccgtt ggcgcgcagt
    47401 tcaacattcc gtacatccat gtgctggtga ataacgccta tctcggcctg attcgtcagt
    47461 cacaacgcgc ttttgacatg gactactgcg tgcaactcgc tttcgagaat atcaactcca
    47521 gtgaagtgaa tggctacggt gttgaccacg taaaagtagc ggaaggttta ggttgtaaag
    47581 ctattcgggt cttcaaaccg gaagatattg cgccagcctt tgaacaggcg aaagccttaa
    47641 tggcgcaata tcgggtaccg gtagtcgtgg aagttattct cgagcgtgtg accaatattt
    47701 cgatgggcag cgaactggat aacgtcatgg aatttgaaga tatcgccgat aacgcagcgg
    47761 atgcaccgac tgaaacctgc ttcatgcact atgaataagg gagataaatg atgttacgtt
    47821 tctctgctaa tttatcgatg ttatttggtg aatatgattt tctcgcccgt tttgagaaag
    47881 ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc ttatgactac gacattgaag
    47941 aattaaaaca ggtactggcg agtaataaac tcgaacatac gctgcacaat ttaccggcag
    48001 gtgactgggc ggcgggcgag cgcggtattg cctgtattcc tggccgtgaa gaagagtttc
    48061 gggatggcgt agcagcagcg att
//
LOCUS       DAZPDE010000013        47723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPDE010000013 DAZPDE010000000
VERSION     DAZPDE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.013"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            134..766
                     /gene="comR"
                     /locus_tag="ACHU0J_000642"
     CDS             134..766
                     /gene="comR"
                     /locus_tag="ACHU0J_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1215468.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(828..1367)
                     /locus_tag="ACHU0J_000643"
     CDS             complement(828..1367)
                     /locus_tag="ACHU0J_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1215469.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1594..2898)
                     /gene="ndh"
                     /locus_tag="ACHU0J_000644"
     CDS             complement(1594..2898)
                     /gene="ndh"
                     /locus_tag="ACHU0J_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1215470.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3306..3848)
                     /gene="ycfP"
                     /locus_tag="ACHU0J_000645"
     CDS             complement(3306..3848)
                     /gene="ycfP"
                     /locus_tag="ACHU0J_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1215471.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3871..4896)
                     /gene="nagZ"
                     /locus_tag="ACHU0J_000646"
     CDS             complement(3871..4896)
                     /gene="nagZ"
                     /locus_tag="ACHU0J_000646"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1215472.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4907..5731)
                     /gene="thiK"
                     /locus_tag="ACHU0J_000647"
     CDS             complement(4907..5731)
                     /gene="thiK"
                     /locus_tag="ACHU0J_000647"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1215473.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5712..6353)
                     /gene="lpoB"
                     /locus_tag="ACHU0J_000648"
     CDS             complement(5712..6353)
                     /gene="lpoB"
                     /locus_tag="ACHU0J_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1215474.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6368..6745)
                     /locus_tag="ACHU0J_000649"
     CDS             complement(6368..6745)
                     /locus_tag="ACHU0J_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1215475.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6748..7107)
                     /gene="hinT"
                     /locus_tag="ACHU0J_000650"
     CDS             complement(6748..7107)
                     /gene="hinT"
                     /locus_tag="ACHU0J_000650"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1215476.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7440..9625
                     /gene="fhuE"
                     /locus_tag="ACHU0J_000651"
                     /pseudo
     CDS             7440..9625
                     /gene="fhuE"
                     /locus_tag="ACHU0J_000651"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9685..11118)
                     /gene="ptsG"
                     /locus_tag="ACHU0J_000652"
     CDS             complement(9685..11118)
                     /gene="ptsG"
                     /locus_tag="ACHU0J_000652"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1215477.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11413..12210)
                     /locus_tag="ACHU0J_000653"
     CDS             complement(11413..12210)
                     /locus_tag="ACHU0J_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1215478.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12221..13225)
                     /gene="holB"
                     /locus_tag="ACHU0J_000654"
     CDS             complement(12221..13225)
                     /gene="holB"
                     /locus_tag="ACHU0J_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1215479.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13222..13863)
                     /gene="tmk"
                     /locus_tag="ACHU0J_000655"
     CDS             complement(13222..13863)
                     /gene="tmk"
                     /locus_tag="ACHU0J_000655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1215480.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13853..14875)
                     /gene="yceG"
                     /locus_tag="ACHU0J_000656"
     CDS             complement(13853..14875)
                     /gene="yceG"
                     /locus_tag="ACHU0J_000656"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1215481.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14878..15687)
                     /gene="pabC"
                     /locus_tag="ACHU0J_000657"
     CDS             complement(14878..15687)
                     /gene="pabC"
                     /locus_tag="ACHU0J_000657"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1215482.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15807..17048)
                     /gene="fabF"
                     /locus_tag="ACHU0J_000658"
     CDS             complement(15807..17048)
                     /gene="fabF"
                     /locus_tag="ACHU0J_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1215483.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17135..17371)
                     /gene="acpP"
                     /locus_tag="ACHU0J_000659"
     CDS             complement(17135..17371)
                     /gene="acpP"
                     /locus_tag="ACHU0J_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1215484.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17582..18316)
                     /gene="fabG"
                     /locus_tag="ACHU0J_000660"
     CDS             complement(17582..18316)
                     /gene="fabG"
                     /locus_tag="ACHU0J_000660"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1215485.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18329..19258)
                     /gene="fabD"
                     /locus_tag="ACHU0J_000661"
     CDS             complement(18329..19258)
                     /gene="fabD"
                     /locus_tag="ACHU0J_000661"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1215486.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19274..20227)
                     /gene="fabH"
                     /locus_tag="ACHU0J_000662"
     CDS             complement(19274..20227)
                     /gene="fabH"
                     /locus_tag="ACHU0J_000662"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1215487.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20295..21365)
                     /gene="plsX"
                     /locus_tag="ACHU0J_000663"
     CDS             complement(20295..21365)
                     /gene="plsX"
                     /locus_tag="ACHU0J_000663"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1215488.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21446..21619)
                     /gene="rpmF"
                     /locus_tag="ACHU0J_000664"
     CDS             complement(21446..21619)
                     /gene="rpmF"
                     /locus_tag="ACHU0J_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1215489.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21671..22192)
                     /gene="yceD"
                     /locus_tag="ACHU0J_000665"
     CDS             complement(21671..22192)
                     /gene="yceD"
                     /locus_tag="ACHU0J_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1215490.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22391..22975
                     /gene="yceF"
                     /locus_tag="ACHU0J_000666"
     CDS             22391..22975
                     /gene="yceF"
                     /locus_tag="ACHU0J_000666"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1215491.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23087..24046)
                     /gene="rluC"
                     /locus_tag="ACHU0J_000667"
     CDS             complement(23087..24046)
                     /gene="rluC"
                     /locus_tag="ACHU0J_000667"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1215492.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24619..27810
                     /gene="rne"
                     /locus_tag="ACHU0J_000668"
     CDS             24619..27810
                     /gene="rne"
                     /locus_tag="ACHU0J_000668"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1215493.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(28007..28949)
                     /gene="flgL"
                     /locus_tag="ACHU0J_000669"
                     /pseudo
     CDS             complement(28007..28949)
                     /gene="flgL"
                     /locus_tag="ACHU0J_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28961..30603)
                     /gene="flgK"
                     /locus_tag="ACHU0J_000670"
                     /pseudo
     CDS             complement(28961..30603)
                     /gene="flgK"
                     /locus_tag="ACHU0J_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30669..31610)
                     /gene="flgJ"
                     /locus_tag="ACHU0J_000671"
     CDS             complement(30669..31610)
                     /gene="flgJ"
                     /locus_tag="ACHU0J_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1215494.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31610..32707)
                     /gene="flgI"
                     /locus_tag="ACHU0J_000672"
     CDS             complement(31610..32707)
                     /gene="flgI"
                     /locus_tag="ACHU0J_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1215495.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32719..33417)
                     /gene="flgH"
                     /locus_tag="ACHU0J_000673"
     CDS             complement(32719..33417)
                     /gene="flgH"
                     /locus_tag="ACHU0J_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1215496.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33470..34252)
                     /gene="flgG"
                     /locus_tag="ACHU0J_000674"
     CDS             complement(33470..34252)
                     /gene="flgG"
                     /locus_tag="ACHU0J_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR1215497.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34390..34977)
                     /gene="flgF"
                     /locus_tag="ACHU0J_000675"
                     /pseudo
     CDS             complement(34390..34977)
                     /gene="flgF"
                     /locus_tag="ACHU0J_000675"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(35079..35141)
                     /locus_tag="ACHU0J_000676"
                     /pseudo
     CDS             complement(35079..35141)
                     /locus_tag="ACHU0J_000676"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35161..36300)
                     /locus_tag="ACHU0J_000677"
     CDS             complement(35161..36300)
                     /locus_tag="ACHU0J_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1215498.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(36325..37020)
                     /gene="flgD"
                     /locus_tag="ACHU0J_000678"
     CDS             complement(36325..37020)
                     /gene="flgD"
                     /locus_tag="ACHU0J_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1215499.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37032..37435)
                     /gene="flgC"
                     /locus_tag="ACHU0J_000679"
                     /pseudo
     CDS             complement(37032..37435)
                     /gene="flgC"
                     /locus_tag="ACHU0J_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37439..37855)
                     /gene="flgB"
                     /locus_tag="ACHU0J_000680"
     CDS             complement(37439..37855)
                     /gene="flgB"
                     /locus_tag="ACHU0J_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1215500.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38010..38669
                     /gene="flgA"
                     /locus_tag="ACHU0J_000681"
     CDS             38010..38669
                     /gene="flgA"
                     /locus_tag="ACHU0J_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1215501.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38745..39038
                     /gene="flgM"
                     /locus_tag="ACHU0J_000682"
     CDS             38745..39038
                     /gene="flgM"
                     /locus_tag="ACHU0J_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1215502.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39043..39459
                     /gene="flgN"
                     /locus_tag="ACHU0J_000683"
     CDS             39043..39459
                     /gene="flgN"
                     /locus_tag="ACHU0J_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1215503.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39499..41034)
                     /gene="murJ"
                     /locus_tag="ACHU0J_000684"
     CDS             complement(39499..41034)
                     /gene="murJ"
                     /locus_tag="ACHU0J_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1215504.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41201..42067)
                     /locus_tag="ACHU0J_000685"
                     /pseudo
     CDS             complement(41201..42067)
                     /locus_tag="ACHU0J_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42069..42716)
                     /locus_tag="ACHU0J_000686"
     CDS             complement(42069..42716)
                     /locus_tag="ACHU0J_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR1215505.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42727..43311)
                     /gene="rimJ"
                     /locus_tag="ACHU0J_000687"
     CDS             complement(42727..43311)
                     /gene="rimJ"
                     /locus_tag="ACHU0J_000687"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1215506.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43547..44755
                     /gene="mdtH"
                     /locus_tag="ACHU0J_000688"
     CDS             43547..44755
                     /gene="mdtH"
                     /locus_tag="ACHU0J_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1215507.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44819..45466
                     /gene="grxB"
                     /locus_tag="ACHU0J_000689"
     CDS             44819..45466
                     /gene="grxB"
                     /locus_tag="ACHU0J_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1215508.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45600..46160
                     /locus_tag="ACHU0J_000690"
     CDS             45600..46160
                     /locus_tag="ACHU0J_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1215509.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46266..47312
                     /gene="pyrC"
                     /locus_tag="ACHU0J_000691"
     CDS             46266..47312
                     /gene="pyrC"
                     /locus_tag="ACHU0J_000691"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1215510.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47386..47631
                     /gene="dinI"
                     /locus_tag="ACHU0J_000692"
     CDS             47386..47631
                     /gene="dinI"
                     /locus_tag="ACHU0J_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1215511.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11571 a        12879 c        11824 g        11449 t
ORIGIN      
        1 cattaattaa tttaaaaatc tttaaatttc agtcaaatag aaagcaatta tttttatggg
       61 atctggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
      121 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc
      181 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
      241 ttatgaagcg acttctcttg cggacctcgt cgaagcaacc ggagccaaag cgcccacgct
      301 atacgcggaa tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga
      361 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
      421 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
      481 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tagccaatac
      541 gttgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
      601 agcgcgcggg gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg
      661 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
      721 gattgccgga acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaggc
      781 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
      841 ttcaaaatca gttgcccgtt actatctagc gggatctgcg tacccggatc gcggtccatg
      901 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
      961 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
     1021 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg
     1081 acatctttac cgcgaccacc accaaactga tgccctatca cgccaccagc aacagcgccg
     1141 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
     1201 ttgcgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
     1261 tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac
     1321 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct
     1381 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
     1441 aaaagcgcgg gaatgactgg aaatacttaa ggattcggag gagtctgaag gaatgcctga
     1501 tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
     1561 ggcgtttata ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat
     1621 aacgcggtta atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc
     1681 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
     1741 cattgagccg cgcgtcaggt tacccatcag gctaccaacg gtggagaagt tcgacagcga
     1801 taccagcgaa ccatgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
     1861 cagaatgttg ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac gcggcggaac
     1921 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc
     1981 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttag tttcaagacc
     2041 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccatacca tcagatcagc
     2101 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
     2161 ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg
     2221 tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc
     2281 gttggtcagg cctttgtaac cgtagctgtg cagttgcttg atcgcgttgt gcaattcagc
     2341 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgttcactt tgccatttgc
     2401 gcccaggttg gcagagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
     2461 ctggtgcggg ttatcgagga aaatgcagtt ctctttgacg cctggcgtat tgaaatcgtt
     2521 agaggtgcta cccagcgcca tgaccagggt gtcataggcg attttacgtt ccggaaccaa
     2581 cagttcacct ttctcgtcgc gtagttctgc aatagtgatt gttttcgctt cacgatcaat
     2641 atcaatgacg gaacccaact ggaactggaa accatgattg cgcgcatggg ccagatagct
     2701 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gtggtttcca
     2761 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttac gtcccagctt
     2821 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatctt
     2881 tttcaatggc gtagtcaacg tgcccccctt aaatttatta accaattgtt aattaatggt
     2941 tattaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga
     3001 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctt
     3061 aaaagatagg caactttacc gggggagatc aaaaaataga gtttattgtt tattatttga
     3121 gcaaattcgg aagggtaata tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat
     3181 aagatgcgca gcatcgcatc aggcattgat gctcgactgt cggatgcggc gtggacgcct
     3241 tatccgactt acagaattcg gagcgcatca ggtaccggca aggttacctg atgcgacgag
     3301 ggcatttacc cgagggtttt gaacgcttta atgcgctgta aatgcgggga gatattcttg
     3361 aatttgtgcg tctgctcttc gtcccagaca atctcgtaat aatgatgcaa ctcttcagat
     3421 gtccgctggc tgttaagtgc ttcatcatta cgcgacaaaa taaccagaca acgatcgcga
     3481 ttcttctcgc ggaagttggt cacacactta gtggcaatat cggcatactc ttccgggcga
     3541 tcaatcttgc cttccatgtt ctcataaggg aacaaattag ggttgaagat cacctggcgg
     3601 atatcgcaaa gaaaaccaat ccgttccgcc cagtatccgc ccaggccaac gccgcaaatt
     3661 agcggacgct cgtcaacgtt cagttgcaac attttgtcca cttctttaag cagatgctgc
     3721 atatcatgtt tcggatgccg cgtactgtag cttatcaagc gtacatccgg gtcaataaac
     3781 tgcaattgta agactttctc gtggttaccc ggactgttag agtcaaaacc gtgtaaatag
     3841 ataatcatcg cttcctcaca taagccaggg ttagtgacct actttctctt cctgccagcg
     3901 ttcatgtaac tgattcagac gggtgctgat cgctttccag cgagccgagt ccatcagttc
     3961 ctgtcgcgaa aatgaacctt tatgatacaa acgtgtaaca cgttctgcct tgatcggtga
     4021 cagattatct aacacgctga cggccccttt acgattattg cagaccagga tcatatcgca
     4081 acccgcatcc agtgatgcct gcccgcgttc ggcataactg cccataatcg cggcaccttc
     4141 catcgataaa tcgtcagaga aaatcacgcc gtcaaacccc agttcctgac gcaaaacggt
     4201 tttcagccag tagggagaac cgctcgccgg acgcggatca acatcactgt agatcacatg
     4261 cgcaggcata atggcgtcga gtttattttc gcggattaag gaactgaaga ccgacatatc
     4321 tttagcgcga atctccgctt gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc
     4381 cgttactgca ccgtgtcctg ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat
     4441 accatcaata aaccggctgg caattgccag ggctttttgt ggatcggcat gataagaacg
     4501 ctcgccaatc gccgcgctga tatgcccgac atccagcact ggcgcaaagc tgatatcaat
     4561 atccatagcg atcatttcgc tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc
     4621 ctcttccatt cccaacagcg cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc
     4681 gcgaaaacgc tgcacgcgtc caccttcctg atctaccgcc accaccagat gattgcgcga
     4741 tgctgcgcgg atctggcgca ccagttcacg taactgggca ggatcatgat agttacgcgt
     4801 aaagagaatc agccctccca ccagcggatg cgccagtatt tcacgctctt ccgcgtccag
     4861 ttcgtaacct tcgacatcca acattactgg acccacactg ctctccttat tgttttatta
     4921 atagctgccg ccaggtgtca tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc
     4981 agcggtactc aaaccaccct cctttgagca tcagcagcca gggaaaccat cgcctgacct
     5041 gacgccataa ttgcgccgga taaatcttcg cgcgagtggc atagtcattg accaattgcc
     5101 ggtgctgttc agtattttcc acccacaccg ccgccagttc cagcgcgata tcaccatctc
     5161 cagcatactc ccagtcgatg agttttaacc ctgatgcgct atgcactaaa tttccggcgt
     5221 ggacatccat atgcaatgga cttaagcgta aaggtcgtgg ttcccgcgct ttgcgcagac
     5281 gttttaacat tcgcagccaa cccacagtcc gccgcgccgg atcgctttgc tgccagtaca
     5341 gttccagtaa gggcaacagc gttattcgcc agccaaaacg tggttgttga tgtagataat
     5401 acagcaagcc tgccagttcg ttggtatccg gcaaatacgt ttttaccgca ccgggcagat
     5461 agtcgactac catccagtca cggagatata aatgcggctt cggtgcaatg cttgcgggta
     5521 gttgtgataa agcccgatac tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg
     5581 gctgacgcac aacaaaacgc tggccctgat gttcaatgag aaaactaccg ccactaagcc
     5641 cactattaaa cgtcgtgacg ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc
     5701 gcgtgatggg attattgctg cgaaacggca cctttacctg accagataat ttcgcccgtc
     5761 tgcaccagca tcagctgcat ttgtagggtc ggagcgttaa cgttgccaga ggcgcaggag
     5821 tacagcacgt aatgagcgcc gacattgcgg gcaatgccta tggctttact acgggtgcct
     5881 aaactgtcct gcggcgacaa acctaactgt tgcttagcca tcgacagttg ctgggcggaa
     5941 accagggtaa atttcccgtt attagccagc gcatttcgca gcgtttcggt cgcttctgcg
     6001 gcattcagcg aaccgttagt acggttgtta acgctatcaa ccagcaggac gctacccgca
     6061 gtcaccccgt cagccccaag catcttactg accatcggct gcattgcgcc attccagtca
     6121 taatggcgaa tatgcggcgc aggcggtgcc gttcgatctt cgtgctcaat tgggcctggc
     6181 tgctgcggga tcgtcggcac cgagggcact gtggggacag gctgttgtgg ctcggctggt
     6241 tgttccggcg ctggtttcac ttcttctacc ggtgcaggtt cacgttgccc cacacacccg
     6301 gcgagaaaca tcgccagcgc ggtaatcaag gcgtagcgac tcatttttgt catcaagatt
     6361 caccccttta caaatataga taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc
     6421 cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat
     6481 gcatctccag ccctctggcg tcataccagt aaaaacgata atgaacggta acgggttctt
     6541 gcctttcgtt ataaatcgtt gaggatgctg aaggttgaat atcagacgtc gaaaggacgg
     6601 gcttttctgc actgatgccc gcagccaata aagatgactc catcaccagc gattgctcat
     6661 cattcaccgg aatttccgga tgtgaacgac agcccaccag cagtaacaac accagagaca
     6721 ccagcccaaa gcatcctttt ctcatcgtta cagaccttta tgcgccagca ttggtcccag
     6781 cggacggcca cccaacaagt gcatatggat gtggtaaacc tcttgtccgc catggcggtt
     6841 ggtattcatg atcagacgat agccatcttc ggcaatacct tcttgctcag caatttttgc
     6901 cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca gctgacacgt cgttcacagt
     6961 cggaatgaga atattcggaa tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg
     7021 aaacgccgtt accagatcat cctggtagac gatatcggag gggatctcac gacgaataat
     7081 tttgctgaat atagtttctt ctgccacgac attttccttt ttcaaatagc catgcgttgc
     7141 gccatgctac gccgcgccac actgcgagta tagagtatgg tcgagttaaa cctgctcttt
     7201 caatcttaac tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt
     7261 attcccattt ctgcctgcga atgcgctgaa aagacattct ctgtttggtt tacacgacaa
     7321 atatgaatgc gtatatttct catttgcatt tgcagcaaaa ttattcgcac actaaatata
     7381 accgctcatc accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa
     7441 tgctttcaac acaatttaac agggataatc aatatcaagt catcaccaaa ccgtcactac
     7501 ttgccggttg catagcactg gcactattac cttctgccgc ttttgctgca ccagccactg
     7561 aagaaacggt gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca
     7621 gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac tcaacatgat attcctcagt
     7681 cggtcactat tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag
     7741 tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt
     7801 attcccgcgg attccagatc gataactata tggttgatgg tatccccacc tattttgaat
     7861 cgcgctggaa tctgggcgac gcactttctg atatggcact gtttgaacgc gtagaagtag
     7921 tgcgtggcgc gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg
     7981 ttcgaaaaca cgcgacctgt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct
     8041 ggaacaaaga acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc
     8101 gcgcgcgaat tgtcggcggc taccagaata acgactcatg gctggaccgc tacaatagtg
     8161 aaaagacctt cttctcgggc attgtcgatg ctgatttagg cgatcttacg acgctttcag
     8221 ccggttacga atatcagcgc attgatgtta atagcccaac ctggggcggt ttaccgcgct
     8281 ggaatactga tggcagcagc aacagttacg atcgcgcacg cagtaccgca cctgactggg
     8341 cgtacaacga taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata
     8401 cctggcaagc gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt
     8461 atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg
     8521 gacctggctt tgatcatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg
     8581 cgctggattt gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctaatgt
     8641 ttggtggcag ttacagcaaa caaaacaatc gttcagttca tgggccaaca tcttcccgga
     8701 tgaaattggc agtttctaca actttaatgg caatttccca caagccgact ggtcatcaca
     8761 gagcctggcg caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac
     8821 ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac
     8881 gctgacttac agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat
     8941 caatgacaac tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg
     9001 tgacagttca ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa
     9061 atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga
     9121 taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa
     9181 agcggtggat gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga
     9241 caactggcag ctgacatttg gcgcaacgcg ctatcttgca gaggataacg aaggaaacgc
     9301 cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt
     9361 catgccagag ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac
     9421 cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt
     9481 cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga
     9541 caaaacctac gataccaaca tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag
     9601 cattaccggc acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc
     9661 ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt
     9721 caggttatcg gatttagtac cgaaaatagc ctgaacacca gaaccagcaa ccactacgcc
     9781 cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac
     9841 gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc
     9901 aaccagagcc ggtgccattt cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc
     9961 acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac
    10021 aatcgcataa ccgataccga cgatcggtaa cagccacagt ttgctgctgt taccagacag
    10081 aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat
    10141 acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac
    10201 gaacatgaag aagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc
    10261 ggagatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc
    10321 aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc
    10381 agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt
    10441 ggtgtattca ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg
    10501 taccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc
    10561 agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac
    10621 aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa
    10681 gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcaat
    10741 cgcaccggag ataatccctc cgagtacgcc agtatccgcc aagtgtttag aggcgatttc
    10801 ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc
    10861 ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac
    10921 accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac
    10981 atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc
    11041 tgcgataggt agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc
    11101 aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct
    11161 cacgcgtggc aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta
    11221 ttacacagag taaaataatt cagtgccaat ttgtttgacg gctatcacgt ttcaacttca
    11281 gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgcggtt
    11341 attcaccgcc caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt
    11401 aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc
    11461 gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat
    11521 gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg
    11581 cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc agggggacat aacgcgcagc
    11641 atcgcgcagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc
    11701 gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc
    11761 gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt
    11821 atggacgata accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc
    11881 ttgctgacgt actttagttt ccggtgtgta ataataatcc agcccggttt cacccagcgc
    11941 tacaacaccc tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg
    12001 gttgagtgga tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg
    12061 catatgtaaa taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc
    12121 ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc
    12181 gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta
    12241 acacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct
    12301 cgcggttgat gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta
    12361 tagcctgcag gcacgaggga gaaagatggt ttgccagttc ggcgaccagg cccggcacat
    12421 caacattggt cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg
    12481 ccagccagtg taaacgcgcc ggagcttgtt catgattaag ggccgccagc agcgaatacc
    12541 agtcgcccga tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt
    12601 tatctccctg aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg
    12661 caagtaatgc atcctgtgac attgtcactt cgcgtgaaag ccaagtcacg gcgtactgtt
    12721 ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt
    12781 caggctcgcg ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca
    12841 gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg
    12901 caccacctag gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc gcatcaatgc
    12961 ccagcgtatt tttccctttt tcgggagcca gggtgtagta atcgggatgc gtgccagcct
    13021 gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga
    13081 gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa
    13141 tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag
    13201 gtcgtaacca tggataccat ctcatgcgtc caactccttc acccagtggg tcacggtagt
    13261 gcggattgca tccatcacgg cctccagcgg ctgggtggca tcaatggtat gaatactttt
    13321 atcttgtgca gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc
    13381 ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt
    13441 aacatcgaga tagagcgtta agtctgggcg aaaatccccg agaacagcat cacgcagtgt
    13501 tgccagcata tgttggtcaa taccacgtcc tccgccctga tacgcctgag tggaaagatc
    13561 gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca ggtttgatga ccgtttctac
    13621 cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc
    13681 gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc
    13741 aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcgagcgtct caaccaccac
    13801 attacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg
    13861 catttttttc cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat
    13921 tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg
    13981 ccggatgcgc agcagccttc agcgaatccg cccccggcgt agctatcgca cccggcggca
    14041 aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt
    14101 tgccattata acgctctccc atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac
    14161 caatgcgtaa acggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg
    14221 tttctttttc gataattgat gccatcgtca ccagctgatt tttatcttta taaggcagac
    14281 cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg tgaactcgct
    14341 tgagtaacgc gacatcggtg gtattggcgg tatacatcaa ggtgtctggc cagaaccaac
    14401 cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt
    14461 cactcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag tcgctcagac
    14521 gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc aacaatttca
    14581 gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa
    14641 gatccggttc gatacgcagc agccattgaa aaacccgtgg gcgattgatg atcttatcag
    14701 cataaagctg ttcaccgagc gccagacgtc cggtccctgg cttcagggta aatatcgtct
    14761 cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag
    14821 cggcgatacc cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta
    14881 attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggtgac
    14941 atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc
    15001 tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca agctgataag aggattgtgc
    15061 cagtaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg
    15121 cgtataaact acattgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca
    15181 cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat
    15241 caatacttgc tcaagccgat taagatgttt aatacctgca agatgaggat tgcgccccag
    15301 ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg
    15361 ataagccgta acggagagaa tccgcgttgc tggtccgctg ttcaatgtgc tgtaccctcg
    15421 cccgccacta ccgcgactga tcacgacttt cagcacacca ttttgctgtt ctgctgccag
    15481 cgttttcatc tcttgttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca
    15541 agcatcctgt agtcgcagga tatgcgccga taacaaactg actttaccgt cgataactct
    15601 ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc
    15661 ctgcttataa ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc
    15721 agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac
    15781 cttttatatg ggtggaaaat gacaacttag atctttttaa agatcaaaga accattagtg
    15841 ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttca
    15901 tgcggtacga aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga
    15961 acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct
    16021 aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg
    16081 aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc
    16141 gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatt
    16201 gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc
    16261 ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac
    16321 tcttcaagta ccagcatacc ggcaccatcg cccagtacga agccatcacg ctctttatcc
    16381 cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca
    16441 aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca
    16501 gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag
    16561 gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc
    16621 accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc
    16681 atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg
    16741 gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga
    16801 acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag
    16861 ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta
    16921 gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta
    16981 gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc
    17041 ttagacacgt ttgtcctcca aggagggaaa aatgattcta gtgggacaaa aagataaaac
    17101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    17161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    17221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    17281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    17341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    17401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    17461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    17521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    17581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    17641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    17701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    17761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    17821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    17881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    17941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggtta gtttcgataa
    18001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    18061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    18121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcac
    18181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    18241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    18301 ttccttcaaa attcatgatt ttcctctttt aaagctcgag cgccgctgcc atcgctgaag
    18361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    18421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    18481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    18541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    18601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    18661 acggtacgct cactggtaac ggcagcgcgc gttttgcgcc agcagcttta caggcagcgc
    18721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    18781 ttaccggaga aacgacctga ccttctgcag cttcttcaca cgctttcgca ataggcgcat
    18841 catccagacc gatgattgcc gccatagcgc ccgtgccttc cggtacggct tcttgcatga
    18901 acttaccgcg catctcaacc agacgcaccg catcagcgaa atcaatcacg ccagcgcaaa
    18961 ccagcgcgga gtattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    19021 gctgctgcca tacgcgatac agcgcaacag atgcagtcaa cagcgctggc tgggtttgcc
    19081 aggttttatt cagttcttca gctgacccct gctgggtcag cgcccacagg tcgtagccca
    19141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    19201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    19261 tttaatcctt atcctagaaa cgaaccagcg cagagcccca agtgaatcca ccgccaaagg
    19321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    19381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    19441 ccatcgacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    19501 gaaccagcca gtccagttga gaacggtcaa gattattcgc cgccagcgtc tcatcaacga
    19561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    19621 ttgaattctc tggattcacg cggtcggcgt ttggcagcgt cagcaattca ccataactac
    19681 cgtcggcatg cagatgggtg gaaatgattc ccggctcttc agaggcagcc agcaccgcag
    19741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc tgttggatcg caggtgcgcg
    19801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    19861 cggctacgct taatgcatag gtgaaacctg cacaggctgc tgcaacgtca aatgccgggc
    19921 aacctttaat gtccaacatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    19981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    20041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    20101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    20161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    20221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    20281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    20341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    20401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    20461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    20521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    20581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    20641 atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    20701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    20761 accgctgagg catcccgaat actgtcaaga ccctttactt cttcttcacc aatattgagc
    20821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    20881 gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg cccctaaatc gaggaccacc
    20941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    21001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    21061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca tacgcattga actcccacgg
    21121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    21181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    21241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    21301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    21361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    21421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    21481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    21541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    21601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    21661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    21721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    21781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    21841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    21901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    21961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    22021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    22081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    22141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    22201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    22261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    22321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    22381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    22441 ggaaaaactg caaatctctt ttgaatgtgc agcacctgag gtcgacgaaa ccccacgcag
    22501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaatcgc aatctctggc
    22561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    22621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    22681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    22741 aacagaagtg gagccttttg acgtccattt ccgtcaccta agcgaggcgg agattgataa
    22801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    22861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    22921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    22981 tgcgccggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    23041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    23101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    23161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    23221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    23281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    23341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    23401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    23461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    23521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    23581 ttttcgctac cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    23641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    23701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    23761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    23821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    23881 gcttaatacg ttttttgttc acccgcactt cacctttacg caaaatacgg taaatcatac
    23941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    24001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    24061 attagccaac aggattcgcg ccactcattt ttctatgctt atttttagtt ttgcacctta
    24121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    24181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    24241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    24301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    24361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    24421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    24481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    24541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    24601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    24661 cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag tccagggcac
    24721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    24781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    24841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    24901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    24961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    25021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    25081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    25141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    25201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    25261 gttcgcgcct tccgcgatta tttacgtcag gatatcggcg aaatccttat cgataacccg
    25321 aaagtcctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    25381 aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc actaccagat cgagtcgcag
    25441 atcgagtccg ccttccagcg tgaagtccgt ctgccatccg gtggttccat tgttatcgac
    25501 agcaccgaag cgttaacagc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    25561 atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg atgagattgc tcgtcagctg
    25621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gccagtacgc
    25681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    25741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    25801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    25861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    25921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    25981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    26041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    26101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    26161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    26221 ccggatgtac cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    26281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    26341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    26401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    26461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    26521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcaaactgc cgagacgcgt
    26581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagtg
    26641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    26701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    26761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    26821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    26881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    26941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    27001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    27061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    27121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    27181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactgaggtc
    27241 cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag ttgttgaaga aatggctgaa
    27301 gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat
    27361 cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg tgattaccga gtctgatgtt
    27421 gccgtagccc aggaagtcgc agaacacgca gaaccgatgg ttgaaccgca ggaagagacg
    27481 gcagatgcag atattgaaga agttgccgaa actgctgagg ttgtggttgc tgaacctgaa
    27541 gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggtg
    27601 gccgcggttg aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc
    27661 gctccggcac cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact
    27721 tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc
    27781 gctcctgcgc gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta
    27841 actgccaggg cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat
    27901 ccttgctaac caacaatgca aaataggcaa aaaaaatgcc cgccccggcg atcccagagc
    27961 gggcagacaa aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct
    28021 ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt
    28081 gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct
    28141 gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc cagcgactcc agtttgttca
    28201 gctgcgtgcc taattccgcg cgaacggtaa ggacattatt cagcgagttt ttcagtccac
    28261 ggttggtttt atccaatgcc gcagcggcga tttctttatc cgcttcgcta tccgcgaccg
    28321 gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc
    28381 taccgtctgg ttccgctacc gcgttactgg taatactgtc gaaaattttg tcgcccgtat
    28441 gtcctatcac catcgaacgc gaagcatcga cctgttgctt aatactttct gttccgccaa
    28501 cataatcacc gttagcctca ctaaacggcg cagtctctgt tttatatgta gcgcccatta
    28561 ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctgaat atccgtagcc
    28621 agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat tttttcctga
    28681 gcattctgga tagcagtggt gacctggcta agtacactct cttccagtga cactttttga
    28741 gtggcgaaag tacgtgccag cgtgtactgg ctgttttgcg cctgtgcctg ggagagaaca
    28801 acagcttgtg atgcagcaat gggatcgtct gaagggttaa cgactcgctt acccgtcgac
    28861 atctgctcgc cgtacttcat ccattctgcc tgagaattgg tgatgccgcg catgttttgc
    28921 tggtacatca tctgggtact gaaacgcatt ttatctcccc ttagcgaatg ttaatcagcg
    28981 catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa
    29041 aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac tgctgctgat
    29101 tggaaagctg cgtcaccaca ttaccttgcg tggtgctact ggttttcaac gtcgcggttt
    29161 tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc gcaccgccca
    29221 ccgttttact gttgctttgc agatccagca gggcctgacc gttgcggttg tcgctatcac
    29281 ccgcatcttc ttcgctcgcc atcgcgattt tcgcttcgtc ggtgattaat acatccatgt
    29341 tgacgatggc gtcacttact ggtttcagcg tgaagctgtc gttaacggca ggcgttcctg
    29401 taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatctggt gtcgccgtaa
    29461 aagtggtatt gctggcaagg cgggtgacct gccactgatt attatcgaac gagattttgt
    29521 aatctgtcgc cagtaccacg gaggcatcag ttaccgtggc accgatcgca acgtcacctt
    29581 tgtttttcgt gttttgcaga accgcgggct taccgatagt aaagaaatct tcaccagcat
    29641 cgccgttagc atcaaatccg gctttgtgtt ggctgttgaa agcctcggca aatgccagcg
    29701 ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat gtcagaatgc
    29761 cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct gccgtcccat
    29821 caacgtaagc gacagtcgta cgagaagggt cggcgctgga aggaactgcc gccagttgcc
    29881 gcgccgtact tccctgaacc agtgagtaac cattggcatc gtgatgttat aagtaccgcc
    29941 atcctgaacg ctgacttcta cgccaacaat ctggtttaat tcgctcacca gttgatcgcg
    30001 ttgatccagc agattgttag gtgacgcccc tgcccccacg cctgtcaggc gcgagatttg
    30061 atcgttcagg ctggcaattt gtttagcgta attgttgatc tgatcaacgc tggcacctat
    30121 cgcgatattg acctgtttgt cctggtcgcg cagatattga tcggtggttt taaactgatt
    30181 caccaaccct tctgatttcc caatcagcgc ctggcgcgcc gccgggtctt ccgcgttact
    30241 caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg aagaggtact
    30301 ggtggagagc atattgtcga ttttcgacat ctgctcatag cgggcggtca ggccgctact
    30361 ttgcgtctgc gcagcacgta actggttggt aataaacgta tcatactcac gctgcacacc
    30421 agaaacgtag acgccattgc caacccagcc gccagcgccc aacgtgctat tggcctgcgc
    30481 cataatagtg gtttggcggg tatatccggc aacgttatag ctggagatat tattacttgc
    30541 cgtatttaac gccgcctggg ccgcgttcag tccgctcatg gcgttattga tcaagctgga
    30601 catggaggtt ccttataagc cttcaattac agagtattat cggcagcgac ccgccggact
    30661 tgagttattc agaacagatt atcaatgttc atgctgtagg ttttgctcac cttgtcgctt
    30721 atcgatttca tctgctggat catgttggtg agtttacggg catagtgagg atcggtagca
    30781 tagcccgcgt cctgtaggac ctgcgccccc tgttccgcac tcgcggcggt cgtcacggcg
    30841 gcgtagcgcg ggttacgtgt taacagccca acgtaatccg acaatgcttc cagatacgag
    30901 ctgtagaccc gaaacttcgc ttttactttc ttcgcttcgc cgttttcata ttcagtcgtg
    30961 gtgatttcag tgacttgccc tttccagttg ccagaggctt tgacaccaaa caggttatag
    31021 cttggctcgc cgttttcgcg gcggatttgc cgttgccccc aaccagattc aagcgcagcc
    31081 tgagcgagga tcaaatgatg tggcacaccg ctttgctggc ttgccagttg ggcgggcagc
    31141 gagagttgcg cgaggaatgc tctactgtca cccggcagcg aatcatcgta gttacgtggt
    31201 acggcctttt gcaccagctg cgaaagcgtc tggttttgat aacgcaccac ggtttcgagc
    31261 gggaatttca tcggtgctgc tggcgtggac tcctctggca atggttgttc tggcgtcatc
    31321 tgtttaacca tcatctctgc aagccccaga cctttgcccg ccgtcatctg ttgggcaatt
    31381 tgctggtcat acatactggt atacagacga gtgtgctcgc tgctgaacag gccatctttt
    31441 ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga acatcccttc cacctgacgg
    31501 gccaccggac ggatatttgc cgccggatct tcgctcgctt tcgcctttag ttcgttgagt
    31561 gattgcgcat cccaggccgc acttgccagt agtttgctgt cgctgatcat cagatgattt
    31621 ccagttttgc ccgcagacat cccgcacttt gcattgattg caaaatagac atcagatcca
    31681 tcggcgtagc gcccagcgca ttgagcgcac gcaccacgtt attgaggctg gcgctggaac
    31741 gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacaa
    31801 cagtctgtcc accaccaaac ggtgtatctg gctggctgac attggcctga cgattaactg
    31861 ttactgagag atttccctgc gctaccgcgc agctgtcgag ggtcacttcg cgattcatca
    31921 ccaccgaacc ggtgcgcgag ttaatcacta ctttagcgtc ctgcggggtg acattaacct
    31981 gcatattctg gatatcggca aggaaacgga cctgggaact gttgccactt ggtacgcgca
    32041 cctgaatagt ccgcgcatct aacgcggtgg cgctgccata tccacgcacg cggttgatgg
    32101 tgtcagcgat ttgctgcgcc atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat
    32161 tcccgacgcc aaactggctg ggcaattcac gttcaataac cgcaccattg gtgatccgtc
    32221 caccgttcag ttggttaacc tgaacactgc taccgcctgc ggaggctcct gcgccgccaa
    32281 ccagaatatt gccctgcgcc agcgcataca cctgactgtc aacgccctta agcggagtca
    32341 tcaacaacgt acctccacgc aagcttttgg cattacccat ggaagaaacc accacatcga
    32401 tggtttgccc ctgacgtcca aacggaggaa gtgacgctgt caccattacc gcagcgacgt
    32461 tttttagctg catattggtg cccgtcggaa cggtaattcc cagctgtgag agcatgttat
    32521 taagcgtttg tgtggtaaac ggcgtctggg ttgtctggtc accagtgcca tccagcccca
    32581 ctaccaagcc atagccaatc agtgagtttt gccttacccc ctgaacactg gtgagatcgc
    32641 gaatacgctc agcctgagcc gccgtcgtaa ccagtagaag aattaatgca gagagaaatt
    32701 taatcaccac agcctcactt acattggcga caggttaagg aagaaacgct gcaaccagcc
    32761 catattttgc gcttcgttaa tgtagccatt gcctacgtat tcaatacgcg catccgccac
    32821 ctgagtagac ggtacggtat tgctgccgct gatagtgcgt ggattaacca cgcccgagaa
    32881 gcgaataaat tcggtaccct gattaatggc aatctgtttt tcacccacca catgcaggtt
    32941 gccgttgacc agtacctggt caaccgtcac cgtcaacgtg ccgctaaagg tattgctggc
    33001 attggccccg ccctttccgt tgaacgtgtt accaccggag gcttcgacat cggcacgagc
    33061 gttaccaaac agcccctgca aatagcgcgg cacagtatca aagccaaaat tagttttacc
    33121 gtcacggctg gcattcgcag aggagctttt gctggcgctg acgttctcct gcaacacgat
    33181 ggtcagcgta tcgccaatat tgcgtggtcg acgatcttca aacagcggtt gatagccata
    33241 gttaatcggc tgagcagact ggaaaataga accgttggcg acgggcgtcg gaccgggaac
    33301 cggttgtgca ctggtcgccc cctgcaccag cggcgtggag ggtatccagg cgcagccggt
    33361 tagtgaaagc accaacaagc tggaaatggc ataagtatgc gcagcgtttt tttgcatggc
    33421 tatcatcttc aaaaatcagt aaaccggtga acctgccacc ggttatgcct tagagttgcg
    33481 tcagtttttg cagcatctga tcggtggtgg acaccgcttt actgttgatt tcgtaagcgc
    33541 gttgcacctg aatcatattg accagttctt ccgccacgtt gacgttagac gtttcaacat
    33601 acccttgata cagcagtccc gcgccgttca ggcccggcgt gctttcgttc ggtgcaccgg
    33661 aggattgcgt ttcggtgtag aggttttcgc caatgctctc cagtccggta tcattcataa
    33721 aggtggtgag attgagctgc ccaacctgaa ccggagctgc ctggccttgt tgggttacgc
    33781 tgaccacgcc atcacgaccg atggtgatac ttaacgcatt cgccggaatg gtgatcgctg
    33841 gctgcacctg aaaaccacca gccgtcacca gctgcccgtt ctgatccacc tggaaagagc
    33901 cgtcacggat ataggctgat gagccatctg gcaacattac ctggaaaaag ccctgccctt
    33961 taatcgcgac atctttgctg ttattggtct gcgacagatt gccctggctg tgtaagcgtt
    34021 cagtggcgac cgggcgtacc cccgtgccga tttgtaatcc ggagggtaag gtggtttgtt
    34081 cggaagactg tgcccccggc tggcgaatgg tttgataaag cagatcttca aacaccgcgc
    34141 gctgacgctt aaaaccgtta gtactgacgt ttgccaggtt gttggcaatg acgtccatat
    34201 tggtttgctg ggcgtcaagg cccgttttgg cgatccataa tgaactgatc atataatgtc
    34261 ctgtagtgga ttgctgacaa gcagagcaga agcacgcgac agtgtgaatt tcccctcacc
    34321 ctaaccctct ccccaaaggg gcgagggaat agtccgcgcg cttttattgc aatgtcatca
    34381 atcgttcaat taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct
    34441 gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc
    34501 cgcaacggca ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc
    34561 tgcctgcagt accggccctc gcgtggcctg ggtttctgcg cttaaacgaa aaatgccgtc
    34621 gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagt ttcagacgcc ctactggcgc
    34681 aactgtattt gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatcgt
    34741 gatttccgcc ccttccggca cagcaattgg ccccgcctcg cctatcaccg gatgcccctg
    34801 aattgtcagt tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct
    34861 gccgtcagcg gtctgcacgg ccagccatcc tgctgcaacg caacgtccag cgggcgagag
    34921 gtgtaatcca tcttgccggg cgtcatgtct gcacccggtg ttgacgccgt taccagcgtg
    34981 cgcgtgggca gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa ctgcgcgcga
    35041 aaaccgggcg tagaggcatt ggccagatta ctggcggtta ccgcctgttg attcagcgtc
    35101 tggctggctg ctcccatcgc agtataaatt gcgtgatcca ttgagctatc ccgtcagcga
    35161 ttagcgtaag ttaacccgcg tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt
    35221 agacttatag ttacgctggg caacgatcat attgaccagt tctttactga gatcgacgtt
    35281 ggacgcttcc agcgcaccgt tggtcagagt gccaaagttt cccgtcccgg ctgtccccaa
    35341 cagcgccacg ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc
    35401 ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc agttgggttt gttcgttgga
    35461 attgttgccg acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt
    35521 gtagccattc tgggtggttg ccacaatgtt gttagcgccg gttgcgatat tattcgccat
    35581 cgcaccatcc actaatgtgc cattagcatt aaattccagt gttgtcgctg tcttcgcaat
    35641 gctgtttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    35701 aaaatagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    35761 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcact
    35821 ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt
    35881 cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttac
    35941 cgggtaaccc gtcagctgta aaccttgcgt attcaacagg ttacggtttt catccagctt
    36001 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    36061 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    36121 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    36181 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    36241 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    36301 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt
    36361 gccgtaagtg ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag
    36421 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    36481 attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac gaaccgtccc aggtgaaact
    36541 gtgaactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc
    36601 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    36661 cgtggtcaca gccccttctt cactgccggt tcccgcaaga acagttgtgc cggggatcat
    36721 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    36781 aatagatccg agcgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa
    36841 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    36901 caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    36961 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    37021 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    37081 agcacttcaa cattggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg
    37141 acgacatcaa cgttcggcat ttttatgtag ccctttgcat ctgccagcgg attacctggt
    37201 tcataaacca gtttgtccgg ggcctgactt tcttaacatc ggcaaccttt acgccgcctg
    37261 tcgcagcacc tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc
    37321 catcaggacc ggtcacgcta tcagcatttg ccagattact ggccgccacg ttcaggcgct
    37381 gggactgggc agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt
    37441 aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc
    37501 tcatctggta ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg
    37561 tattaccgtc aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggaggcg
    37621 tcagcgcctg cgccgggatg tgctgcgttg aggtcatcgt cagtgcaacc acactggttg
    37681 catcccgtcc acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct
    37741 gataaccagg ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc
    37801 gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc
    37861 ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg
    37921 gaataaacgc aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaat
    37981 tcacgttgta gaaatggctg ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca
    38041 tcgcgatact gttcagtccg ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact
    38101 tttttagcgc ccaactcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc
    38161 ccaatctact accgccatgc gagcagccat tgctttcgat gagcaataat tcccgcctgt
    38221 ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg
    38281 tgcaggccac aggaaattat gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg
    38341 aagctggcaa tgtcaaactg aaacgtggac gcctggatac cctgccaccg cgtacggtgc
    38401 tggatatcaa tcaacttgtt gatgccgtta gcctgcgcga tctatcaccc gatcaaccta
    38461 tccagttaac ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaatgtga
    38521 tcgccagcgg tgatgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg
    38581 tcgcacagaa tgcgcgggtg cgcatggtat cggaacaggt agtcagcggc gttgttgatg
    38641 cagatgggaa tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata
    38701 aataagcaac acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca
    38761 cttcgcctct gaagcctgta agcaccgttc aaccgcgcga aaccaccgac gcgccggtaa
    38821 cgaacagccg ggcggcaaaa acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg
    38881 cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt
    38941 taaaactggc gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc
    39001 tgatcaacga agcgcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag
    39061 atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag
    39121 caacatctct ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa
    39181 agctcactgc tggcgacgct ggattacctc gaacagttac gcaagaaaga acccaataca
    39241 gcaaatagcg ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta
    39301 cgccaaatga atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag
    39361 gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc
    39421 tcaacacccc atcgcggcag taaaaagatt tcgatctgaa tattatctcc ggcctgcact
    39481 gcaggccgga atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag
    39541 taccgccagt gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag
    39601 taagcgccag ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag
    39661 cacgccagac atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg
    39721 ttgcggagta aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca
    39781 cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag
    39841 gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc
    39901 ctggcgggaa taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac
    39961 cgagtaggca attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta
    40021 ctggaacagc gaaacggtta acggacctga aagaatgccc aacgcaaccg cactcggcag
    40081 tgccaacagg aaacaaagac gcaaccccca gtccatcaaa cggttgtatt catcatgatt
    40141 gccactggca aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag
    40201 cacaccggag ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga
    40261 agcaagaaac gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc
    40321 aaggatcgcc ggtcccatct gtttcaccac gcgcattgct ccagcatcgt ggaagttaat
    40381 gcgcggcagg accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag
    40441 aatgccgccg accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc
    40501 ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt gttggcgcaa acgccggaat
    40561 cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa
    40621 gatataggga aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc
    40681 agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt
    40741 cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc
    40801 gtcttcaccc tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc
    40861 cccttcggca aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc
    40921 ggttgccatc cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac
    40981 acgcgaaaac atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt
    41041 tctaatccgg acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc
    41101 tacctagatt atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat
    41161 cgcgccagat cttgtcaacg atacgttgcg ccagcactgc ctattcgccg gcggtttgcg
    41221 gaactgtctg attttacacg cattcaatga agtgccgcgc acagccgaca aatccacgtt
    41281 gctcaagagt gctctgccaa ccaggaatcg gtttattcac tacaccctgc ccgcgctcct
    41341 cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg
    41401 tttcacgctg acttccggca cggcgatgca tacaagtggt gatctgcaaa ggaccggccg
    41461 aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat
    41521 ccagagaggc tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat
    41581 ccagcaacgt gaaataaaga tcgtgtggcc cgacgctatt gctacgatgt ttatccattc
    41641 ttagcgaggc tgcggtggcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac
    41701 ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc cagttccacc agcctttcag
    41761 catcgcgcag attttctgcc agcggtttat cgacacagac atgtaccccc gcattgagta
    41821 acgtactgac cacgtcaaag tggctggcgg tgctggagtg cacaaaaacc gcatcacaac
    41881 tggcggcaag gctggataac gaatcggcat aaggaatgcg ccagctgtca caaattggca
    41941 gggctttggc gcgcgtaggc gaccaggctc cttgtaacgt ccagtcagac gctgccgcca
    42001 gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt
    42061 ttttcacttt aatctcccag atgggccagc aacgaatcaa gacgttgttt cagttctgcc
    42121 acttcgattt ccagggcttc gacgcgggtc tgtaaatcac cgtcaaccat gttcgacata
    42181 gccgttaccg ccggctgatc ttcaacctca ccactgaaaa gatgcatgta gcggctttcg
    42241 cgtttacccg gttcgcgggc cagacgcacc acaaaaggac catcttcgcg attcgccagt
    42301 tgttccagcg tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg
    42361 ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact
    42421 tctgcggcgc tcagtttcag atcgccaaat tctgaattac aaaaacgttg ctcatatttg
    42481 gtgacccgat taccaaaacc gctcactgtg cgtagataat gacgtttgac cagattatcc
    42541 agctgttcct gcacttcgga ttcactcaga ttcatgaccg gttcacggtt cgttttctga
    42601 ttacaggccg tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc
    42661 ttttccagca agcaaccaat tacgcgagct tccagggcag taagttgata tttcatgaga
    42721 ttctccttag cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg
    42781 ccattgtcca tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg
    42841 cgccagtaaa tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat
    42901 atgttgggtg cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt
    42961 gccctgccat ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccgcgaac
    43021 aacattggaa aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa
    43081 gtagaaagct gaaccttgtt tatgaaattc gttaatcatc cctagcctgg cctgccagcc
    43141 tgatggataa cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt
    43201 ctctgcgtaa taatccgcca gacgccaggc atcacgatca tgcaccagac gcacgaccag
    43261 tcggtctgtg gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct
    43321 actccttccc gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc
    43381 ctgtgaaaag tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta
    43441 tcaaagcgac gttttgataa aaaaatactg tccccgaagt gatgggaaaa gtcttagcgg
    43501 caatgcagaa tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc
    43561 gcaggcgagg aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg
    43621 gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc
    43681 cgtcatggtc ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt
    43741 cggcggcgca attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat
    43801 gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc
    43861 atgcctgctc tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt
    43921 gaaactaatc cgtccacaac aacgtggtcg ttttttctcg ctgttgatga tgcaggacag
    43981 tgccagtgcg gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct
    44041 ggtctgcgcc acaggggccg ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt
    44101 accggcatgg aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat
    44161 gcgtgacaag cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt
    44221 acaagtgatg ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt
    44281 taaatggatg tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg
    44341 ctggagtgaa aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc
    44401 attaagcatg atgccggtgg gcatggtcag cggcctacaa caacttttca ccctgatttg
    44461 tctgttttat atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct
    44521 ggcggacgca agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg
    44581 cggcgctatt ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cggcgcacca
    44641 gccagagctt ccgtggatga tgctgggaat tattggcatc ttcactttcc ttgcgctggg
    44701 ttggcagttt agtcagaaac gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga
    44761 tactcccccc tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt
    44821 gaagctatac atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct
    44881 gaaaaatatc cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg
    44941 gatggtcggt caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga
    45001 aagcatggat atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa
    45061 acgttcccct gccattgaag agtggctgcg caaggtcaat ggctacgtca acaaactgct
    45121 gttgccgcgt tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata
    45181 tttcgtcgac aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga
    45241 cggtctgatt aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc
    45301 gaacgccgtg aatggcgaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa
    45361 tctgacgctg gtagccggaa tcaactggcc aagccgcgtt gctaattacc gcgataatat
    45421 ggcgaaacag acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc
    45481 ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc
    45541 tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca
    45601 tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc
    45661 aactcaccca atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata
    45721 atttctcaaa agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact
    45781 tgaccagcca aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc
    45841 tggacatgaa ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc
    45901 tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg
    45961 cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc
    46021 aggcattacg caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc
    46081 agggcgaagc aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg
    46141 tcattccgtt tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag
    46201 gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga
    46261 gattaatgac tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc
    46321 acctccgcga tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac
    46381 gggctatcgt aatgcccaat ctggctccgc ccgtgaccac agttgaggct gccgtggcgt
    46441 atcgccaacg tattcttgac gccgtacctg ccgggcacga tttcacccca ttgatgacct
    46501 gttatttaac agattcgctg gatcctaatg agctggagcg cggatttaat gaagccgtgt
    46561 tcaccgctgc aaaactttac ccggcaaacg caaccactaa ctccagccac ggcgtgacgt
    46621 caattgacgc aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg
    46681 tgcatggtga agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag
    46741 aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca
    46801 tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca
    46861 tcactccgca gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc
    46921 acctgtattg tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg
    46981 tcgccagcgg ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc
    47041 gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt
    47101 acgctaccgt ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa
    47161 acggcccgca gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag
    47221 agcaacaggt tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg
    47281 gggaaacggt acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt
    47341 tattttacct gtataaataa ccagtatatt caacaggggg ctattatgcg aattgaagtc
    47401 accatagcga aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt
    47461 tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttatgcc
    47521 gcagcgaata atttatcggt tattggcgca acaaaagaag ataaacagcg cattagcgaa
    47581 attctccagg aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg
    47641 atttttgttt ttgccggatc gccccggctt ttttatttta cttacgaatt ttgagtacaa
    47701 aatttctaca cttcctcaaa ata
//
LOCUS       DAZPDE010000014        47480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPDE010000014 DAZPDE010000000
VERSION     DAZPDE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.014"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="ACHU0J_000693"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="ACHU0J_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            1316..1441
                     /gene="timP"
                     /locus_tag="ACHU0J_000694"
     CDS             1316..1441
                     /gene="timP"
                     /locus_tag="ACHU0J_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1215512.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACHU0J_000695"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACHU0J_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1215513.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACHU0J_000696"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACHU0J_000696"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1215514.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACHU0J_000697"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACHU0J_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1215515.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACHU0J_000698"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACHU0J_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1215516.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACHU0J_000699"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACHU0J_000699"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1215517.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACHU0J_000700"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACHU0J_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1215518.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACHU0J_000701"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACHU0J_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1215519.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACHU0J_000702"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACHU0J_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1215520.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACHU0J_000703"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACHU0J_000703"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1215521.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACHU0J_000704"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACHU0J_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1215522.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACHU0J_000705"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACHU0J_000705"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1215523.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="ACHU0J_000706"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="ACHU0J_000706"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1215524.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="ACHU0J_000707"
     CDS             11405..12259
                     /locus_tag="ACHU0J_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1215525.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="ACHU0J_000708"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="ACHU0J_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1215526.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="ACHU0J_000709"
     CDS             complement(13722..14861)
                     /locus_tag="ACHU0J_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1215527.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACHU0J_000710"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACHU0J_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1215528.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /gene="hcaE"
                     /locus_tag="ACHU0J_000711"
     CDS             16047..17408
                     /gene="hcaE"
                     /locus_tag="ACHU0J_000711"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1215529.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /gene="hcaF"
                     /locus_tag="ACHU0J_000712"
     CDS             17405..17923
                     /gene="hcaF"
                     /locus_tag="ACHU0J_000712"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1215530.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="ACHU0J_000713"
     CDS             17923..18243
                     /locus_tag="ACHU0J_000713"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1215531.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="ACHU0J_000714"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="ACHU0J_000714"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1215532.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20279
                     /gene="hcaD"
                     /locus_tag="ACHU0J_000715"
     CDS             19062..20279
                     /gene="hcaD"
                     /locus_tag="ACHU0J_000715"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1215533.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20376..20798
                     /locus_tag="ACHU0J_000716"
     CDS             20376..20798
                     /locus_tag="ACHU0J_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1215534.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20846..21718)
                     /locus_tag="ACHU0J_000717"
     CDS             complement(20846..21718)
                     /locus_tag="ACHU0J_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1215535.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21730..22824)
                     /locus_tag="ACHU0J_000718"
     CDS             complement(21730..22824)
                     /locus_tag="ACHU0J_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1215536.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22857..23855)
                     /locus_tag="ACHU0J_000719"
     CDS             complement(22857..23855)
                     /locus_tag="ACHU0J_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1215537.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23880..25391)
                     /locus_tag="ACHU0J_000720"
     CDS             complement(23880..25391)
                     /locus_tag="ACHU0J_000720"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1215538.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25414..26396)
                     /locus_tag="ACHU0J_000721"
                     /pseudo
     CDS             complement(25414..26396)
                     /locus_tag="ACHU0J_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26493..29774)
                     /locus_tag="ACHU0J_000722"
                     /pseudo
     CDS             complement(26493..29774)
                     /locus_tag="ACHU0J_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29892..31085
                     /locus_tag="ACHU0J_000723"
     CDS             29892..31085
                     /locus_tag="ACHU0J_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR1215539.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="ACHU0J_000724"
     CDS             complement(31283..32536)
                     /gene="glyA"
                     /locus_tag="ACHU0J_000724"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1215540.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32863..34053
                     /gene="hmpA"
                     /locus_tag="ACHU0J_000725"
     CDS             32863..34053
                     /gene="hmpA"
                     /locus_tag="ACHU0J_000725"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1215541.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="ACHU0J_000726"
     CDS             complement(34098..34436)
                     /gene="glnB"
                     /locus_tag="ACHU0J_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1215542.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="ACHU0J_000727"
     CDS             complement(34497..35831)
                     /gene="glrR"
                     /locus_tag="ACHU0J_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1215543.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="ACHU0J_000728"
     CDS             complement(35821..36534)
                     /gene="qseG"
                     /locus_tag="ACHU0J_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1215544.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="ACHU0J_000729"
     CDS             complement(36699..38126)
                     /gene="qseE"
                     /locus_tag="ACHU0J_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1215545.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38684..42571)
                     /gene="purL"
                     /locus_tag="ACHU0J_000730"
                     /gene_synonym="purI"
     CDS             complement(38684..42571)
                     /gene="purL"
                     /locus_tag="ACHU0J_000730"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1215546.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42830..44386
                     /gene="mltF"
                     /locus_tag="ACHU0J_000731"
     CDS             42830..44386
                     /gene="mltF"
                     /locus_tag="ACHU0J_000731"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1215547.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEKIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44383..44886)
                     /gene="tadA"
                     /locus_tag="ACHU0J_000732"
     CDS             complement(44383..44886)
                     /gene="tadA"
                     /locus_tag="ACHU0J_000732"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1215548.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="ACHU0J_000733"
     CDS             complement(44944..45579)
                     /gene="yfhb"
                     /locus_tag="ACHU0J_000733"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1215549.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45788..46636
                     /locus_tag="ACHU0J_000734"
     CDS             45788..46636
                     /locus_tag="ACHU0J_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1215550.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46975..47480)
                     /locus_tag="ACHU0J_000735"
                     /pseudo
     CDS             complement(46975..47480)
                     /locus_tag="ACHU0J_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11323 a        13017 c        11944 g        11196 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc
    19621 gtaatgcacc accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc
    19681 gcattctgct caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc
    19741 tgcaaagtgg ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca
    19801 acgagcaact ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg
    19861 cttgccgcac ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg
    19921 ataatggtgc actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg
    19981 ccgctgccgc aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg
    20041 atcagtacag tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc
    20101 gtggcaaccc ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg
    20161 gtgcggtcac gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg
    20221 gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat
    20281 caggataatt agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta
    20341 cgtttaaatc acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt
    20401 ttggagctgc ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca
    20461 ttatttttgg ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt
    20521 tgggcgcgcc aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg
    20581 cgatattaat cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca
    20641 cgctgggtac ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc
    20701 caaatatgat taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa
    20761 ttaccggacc gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg
    20821 aacgccttat ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg
    20881 ttgttgattc ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat
    20941 ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg
    21001 cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg
    21061 cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt
    21121 tcacccactg acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct
    21181 cgcaaaactc acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct
    21241 gaatccgcgt ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg
    21301 tctctgcccc ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa
    21361 acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac
    21421 tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt
    21481 gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt
    21541 tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac
    21601 cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc
    21661 cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc
    21721 gctaactcct taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag
    21781 cacataggca tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca
    21841 cagcttccag tcagtcaaat cgtgggcaca tttttccata tggaacagac tggtcaccca
    21901 ggagccgata atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt
    21961 tccggtttca ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa
    22021 gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc
    22081 agcaattatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa
    22141 ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc
    22201 gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac
    22261 gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca
    22321 actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc
    22381 ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc
    22441 tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga
    22501 aatgtgatac accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc
    22561 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
    22621 tgccgccgcc gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc
    22681 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
    22741 cagatcgacg gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc
    22801 attaacaata cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag
    22861 cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
    22921 atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta
    22981 ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg
    23041 gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
    23101 gcacctgcgt taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat
    23161 aatcccgaaa gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc
    23221 gccgccgtgg cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta
    23281 atgaacacaa acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga
    23341 aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg
    23401 gtcataaaca gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt
    23461 ggtacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc
    23521 aacaccagca gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac
    23581 accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
    23641 agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg
    23701 ttattcaatg agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc
    23761 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
    23821 ttgccctgcg gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat
    23881 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc
    23941 tgcgagaacg tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc
    24001 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg
    24061 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
    24121 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc
    24181 gtgccgatgg gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg
    24241 gtcaggcggc ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg
    24301 gtcagcactg tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt
    24361 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc
    24421 tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc
    24481 aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc
    24541 gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc
    24601 tgatccacaa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt
    24661 agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca
    24721 tcgcgcataa cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca
    24781 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact
    24841 tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
    24901 atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt
    24961 tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc
    25021 aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc
    25081 gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca
    25141 cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
    25201 ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt
    25261 ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
    25321 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
    25381 gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
    25441 cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt
    25501 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat
    25561 tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc
    25621 aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg
    25681 taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc
    25741 cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc
    25801 ctggttagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt
    25861 acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
    25921 aattttattg gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt
    25981 ggatcgccga ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg
    26041 ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca
    26101 ttttcagaca ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca
    26161 aaggtacttt ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact
    26221 gaagaatctt ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta
    26281 tcaaggtata ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta
    26341 cctaatagcg tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg
    26401 cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc
    26461 gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc
    26521 atcacagtgg tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg
    26581 cgagcagact catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc
    26641 aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt
    26701 gaaacagcaa agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc
    26761 atttcactaa acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct
    26821 tgccagaaga gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga
    26881 tcgccggtcg ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg
    26941 gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg
    27001 cttaaattct ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct
    27061 ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc
    27121 ttcccttccc cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg
    27181 tctgcctgac ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc
    27241 agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt
    27301 aacttatcca gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa
    27361 gcattatcaa gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc
    27421 gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag
    27481 gcaatggctt tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca
    27541 aaccaacact cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg
    27601 acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc
    27661 agcaagctgg cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc
    27721 tctgccgcca gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta
    27781 acgtcgcgtc cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg
    27841 aaccagttca gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc
    27901 tgcaaacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc
    27961 acttcctgat tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg
    28021 aagttatcat tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg
    28081 ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg
    28141 taagcactgg cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt
    28201 gcacgtttca gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat
    28261 tccagcatcg ccagcgccag gttacagcga taatccagcg ggtccagcgc cacgccgcgc
    28321 aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata
    28381 aaccaggctt catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc
    28441 ggcaacggca actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca
    28501 tcggagagtt caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg
    28561 gtcgctggtg tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg
    28621 atcgccagac ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc
    28681 tcactacgct ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa
    28741 tgataaggca ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa
    28801 tcaggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg
    28861 gtcagactct tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta
    28921 cctggcgcaa tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac
    28981 gcgccgacaa aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt
    29041 ttatagcgag aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg
    29101 atggggaaag cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg
    29161 aaaacgctct gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga
    29221 cgcggcgtgg cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg
    29281 cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac
    29341 accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc
    29401 gggcgatggt gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc
    29461 cccaccagcg caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta
    29521 tcccatgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg
    29581 ttttccagcc acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc
    29641 acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg
    29701 ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact
    29761 tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg
    29821 cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag
    29881 gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg
    29941 tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg
    30001 cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg
    30061 tggtgaatat tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg
    30121 cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg
    30181 ttgctaatgc ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga
    30241 cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc
    30301 cggaccgcac catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg
    30361 tatcgcaaac catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg
    30421 aagagaagct gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg
    30481 agaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca
    30541 ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac
    30601 agattggtca caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca
    30661 gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat
    30721 cacaaccggt taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg
    30781 cctggcagag tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg
    30841 gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc
    30901 gcagttgtgc ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc
    30961 cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa
    31021 gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga
    31081 tttgatggcg cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa
    31141 aaattgcctg atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg
    31201 caagattttg taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat
    31261 tgtcagcaaa tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa
    31321 ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca
    31381 tccagccagc cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag
    31441 tacctacacg aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt
    31501 tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt
    31561 tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc
    31621 cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag
    31681 ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca
    31741 tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt
    31801 cgctaccacc tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg
    31861 tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc
    31921 ccgcaacgtg cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca
    31981 ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct
    32041 tgtgttcttt ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac
    32101 cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt
    32161 gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt
    32221 agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga
    32281 acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc
    32341 cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg
    32401 cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct
    32461 gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca
    32521 tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat
    32581 aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct
    32641 tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag
    32701 agcttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca
    32761 cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg
    32821 catttgagat acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc
    32881 atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc
    32941 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
    33001 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
    33061 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
    33121 agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg
    33181 gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta
    33241 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
    33301 ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt
    33361 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
    33421 caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac
    33481 tctttgactc gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt
    33541 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
    33601 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
    33661 accggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
    33721 acagcacaag tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat
    33781 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
    33841 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
    33901 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
    33961 ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
    34021 tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg
    34081 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
    34141 acccgagcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
    34201 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
    34261 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
    34321 tggcgaccaa agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt
    34381 gcttcgcgga catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta
    34441 ttccttgaaa aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
    34501 tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg
    34561 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta
    34621 cgcaagtagt tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt
    34681 tcaccctcca gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc
    34741 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc
    34801 aggcgtttca tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc
    34861 tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga
    34921 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
    34981 atcgcttttg acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
    35041 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt
    35101 aacggcgcag gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg
    35161 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
    35221 tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg
    35281 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga
    35341 ccgttaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
    35401 agcatcagcg ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg
    35461 gattgctcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg
    35521 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca
    35581 gtaagaataa ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata
    35641 ccgtccattt catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt
    35701 acccgtaatc cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg
    35761 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
    35821 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg
    35881 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt
    35941 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
    36001 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc
    36061 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
    36121 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
    36181 cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
    36241 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
    36301 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat
    36361 gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
    36421 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg
    36481 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
    36541 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg
    36601 cggagaaacc ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac
    36661 tgacaaataa aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac
    36721 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
    36781 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc
    36841 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
    36901 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
    36961 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
    37021 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
    37081 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc
    37141 ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca
    37201 agcagttgtt cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc
    37261 tctttttgct ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg
    37321 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
    37381 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
    37441 aactcgctcg gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg
    37501 ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc
    37561 atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
    37621 gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc
    37681 atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca
    37741 ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt
    37801 ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
    37861 ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc
    37921 tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg
    37981 cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc
    38041 agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag
    38101 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
    38161 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
    38221 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
    38281 gctggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg
    38341 gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa
    38401 gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc
    38461 tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt
    38521 agattgcaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat
    38581 tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt
    38641 gcttaatgcc gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac
    38701 gcgcattgcg gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg
    38761 agttgctgac agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac
    38821 tttcagtcgt aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt
    38881 cagtgacttt gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg
    38941 ccgccagatg cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg
    39001 gcatttgcga gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca
    39061 ggctgaaacg cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac
    39121 tccccgggat cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg
    39181 ccagcgtttg cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca
    39241 ggatagactt cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc
    39301 acgcgaccag ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat
    39361 gcacgtcgat agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt
    39421 tcacgccctg ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg
    39481 ctgccacatc ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt
    39541 tagatttcgc ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct
    39601 gccaggtagt ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct
    39661 gcccgttggc ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc
    39721 aatccgcaag cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa
    39781 cctgaatcac cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag
    39841 aggcgatatc cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca
    39901 gcaggcctcc atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct
    39961 gaatcgcgtc atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc
    40021 caagctgacg ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca
    40081 aatcaatcag cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta
    40141 catcttccac gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct
    40201 cttcgttacc ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg
    40261 tcaggcccag cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg
    40321 catcttcacc agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga
    40381 tatcgccaat ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag
    40441 aggcggcgaa atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt
    40501 agtagctgtc gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc
    40561 ccaccatctg atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga
    40621 aagttttctc cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga
    40681 tcccttcacg gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct
    40741 tcggcgtttt accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac
    40801 gatcgtgcag agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc
    40861 gctcacgctt acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt
    40921 agcgttcctg ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta
    40981 gaatatcgcg cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg
    41041 cgttagaaag accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa
    41101 gctgccagca acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct
    41161 gtacggaagc aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc
    41221 cgccgccaag accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt
    41281 tgatctcgcc tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga
    41341 tcggtttgtg ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag
    41401 tacggaagta gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca
    41461 gcgggccttc ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt
    41521 cttcccacgg ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac
    41581 cagctttcgg cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac
    41641 cggtcgccgc gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca
    41701 tcaggatgtg cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt
    41761 agcggcccac ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt
    41821 gatctggcgt ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt
    41881 caccgtcgat aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct
    41941 gagcgaacat atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct
    42001 gcagatagtc aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca
    42061 gcgcctgacg gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg
    42121 caaacaactg ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca
    42181 gttcagcggt aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag
    42241 caacgccgcg ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg
    42301 ctttcgaaga ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt
    42361 gcggggcgtg gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct
    42421 catcatcgtt taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt
    42481 gaaccgggag cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca
    42541 gtgcaggcga accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt
    42601 acccaagggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    42661 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    42721 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    42781 tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt
    42841 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
    42901 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
    42961 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
    43021 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
    43081 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
    43141 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
    43201 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
    43261 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct
    43321 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
    43381 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
    43441 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
    43501 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
    43561 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
    43621 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
    43681 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaaa aaattgactg
    43741 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
    43801 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
    43861 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
    43921 gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta
    43981 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
    44041 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
    44101 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
    44161 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
    44221 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
    44281 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
    44341 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
    44401 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
    44461 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
    44521 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
    44581 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
    44641 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
    44701 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
    44761 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
    44821 ggcacgtttc gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc
    44881 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
    44941 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
    45001 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
    45061 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
    45121 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
    45181 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
    45241 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
    45301 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
    45361 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
    45421 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
    45481 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
    45541 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
    45601 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
    45661 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
    45721 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
    45781 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga
    45841 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
    45901 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
    45961 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
    46021 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
    46081 cggcgaaaaa ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag
    46141 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
    46201 cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg
    46261 cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
    46321 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
    46381 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
    46441 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
    46501 cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
    46561 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
    46621 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
    46681 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa
    46741 tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt
    46801 ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt
    46861 taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa
    46921 cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat
    46981 aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac
    47041 cttctggcga gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga
    47101 aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt
    47161 tgttcgttca acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa
    47221 aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc
    47281 tcgttcgcgc aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc
    47341 cagcttccag acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc
    47401 cggtctttcg gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca
    47461 gttcaacgca ttacgttgta
//
LOCUS       DAZPDE010000015        46617 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPDE010000015 DAZPDE010000000
VERSION     DAZPDE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.015"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            72..1760
                     /locus_tag="ACHU0J_000736"
     CDS             72..1760
                     /locus_tag="ACHU0J_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR1215551.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            1757..2671
                     /locus_tag="ACHU0J_000737"
     CDS             1757..2671
                     /locus_tag="ACHU0J_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1215552.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2704..2952
                     /locus_tag="ACHU0J_000738"
     CDS             2704..2952
                     /locus_tag="ACHU0J_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1215553.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2952..4123
                     /locus_tag="ACHU0J_000739"
                     /pseudo
     CDS             2952..4123
                     /locus_tag="ACHU0J_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4159..5238)
                     /gene="lptG"
                     /locus_tag="ACHU0J_000740"
     CDS             complement(4159..5238)
                     /gene="lptG"
                     /locus_tag="ACHU0J_000740"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1215554.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5235..6305)
                     /gene="lptF"
                     /locus_tag="ACHU0J_000741"
     CDS             complement(5235..6305)
                     /gene="lptF"
                     /locus_tag="ACHU0J_000741"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1215555.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6336..6896)
                     /locus_tag="ACHU0J_000742"
     CDS             complement(6336..6896)
                     /locus_tag="ACHU0J_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1215556.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6908..7732)
                     /locus_tag="ACHU0J_000743"
     CDS             complement(6908..7732)
                     /locus_tag="ACHU0J_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215557.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7732..9081)
                     /locus_tag="ACHU0J_000744"
     CDS             complement(7732..9081)
                     /locus_tag="ACHU0J_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1215558.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9127..9885)
                     /locus_tag="ACHU0J_000745"
     CDS             complement(9127..9885)
                     /locus_tag="ACHU0J_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215559.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(9917..10630)
                     /locus_tag="ACHU0J_000746"
     CDS             complement(9917..10630)
                     /locus_tag="ACHU0J_000746"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1215560.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10804..11496
                     /locus_tag="ACHU0J_000747"
     CDS             10804..11496
                     /locus_tag="ACHU0J_000747"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1215561.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11545..13044)
                     /gene="pitB"
                     /locus_tag="ACHU0J_000748"
     CDS             complement(11545..13044)
                     /gene="pitB"
                     /locus_tag="ACHU0J_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1215562.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13336..15195)
                     /gene="gss"
                     /locus_tag="ACHU0J_000749"
     CDS             complement(13336..15195)
                     /gene="gss"
                     /locus_tag="ACHU0J_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1215563.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15400..16266
                     /gene="yghU"
                     /locus_tag="ACHU0J_000750"
     CDS             15400..16266
                     /gene="yghU"
                     /locus_tag="ACHU0J_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1215564.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16295..16376)
                     /locus_tag="ACHU0J_000751"
                     /pseudo
     CDS             complement(16295..16376)
                     /locus_tag="ACHU0J_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16389..16637)
                     /gene="hybG"
                     /locus_tag="ACHU0J_000752"
     CDS             complement(16389..16637)
                     /gene="hybG"
                     /locus_tag="ACHU0J_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1215565.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16771..17112)
                     /gene="hypA"
                     /locus_tag="ACHU0J_000753"
     CDS             complement(16771..17112)
                     /gene="hypA"
                     /locus_tag="ACHU0J_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1215566.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17105..17593)
                     /gene="hybE"
                     /locus_tag="ACHU0J_000754"
     CDS             complement(17105..17593)
                     /gene="hybE"
                     /locus_tag="ACHU0J_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1215567.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17586..18080)
                     /locus_tag="ACHU0J_000755"
     CDS             complement(17586..18080)
                     /locus_tag="ACHU0J_000755"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1215568.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18080..19783)
                     /gene="hybC"
                     /locus_tag="ACHU0J_000756"
     CDS             complement(18080..19783)
                     /gene="hybC"
                     /locus_tag="ACHU0J_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1215569.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19780..20958)
                     /gene="hybB"
                     /locus_tag="ACHU0J_000757"
     CDS             complement(19780..20958)
                     /gene="hybB"
                     /locus_tag="ACHU0J_000757"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1215570.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20948..21934)
                     /gene="hybA"
                     /locus_tag="ACHU0J_000758"
     CDS             complement(20948..21934)
                     /gene="hybA"
                     /locus_tag="ACHU0J_000758"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1215571.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21937..23055)
                     /gene="hybO"
                     /locus_tag="ACHU0J_000759"
     CDS             complement(21937..23055)
                     /gene="hybO"
                     /locus_tag="ACHU0J_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1215572.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23244..23531)
                     /gene="yghW"
                     /locus_tag="ACHU0J_000760"
     CDS             complement(23244..23531)
                     /gene="yghW"
                     /locus_tag="ACHU0J_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1215573.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23650..24537)
                     /gene="yghX"
                     /locus_tag="ACHU0J_000761"
     CDS             complement(23650..24537)
                     /gene="yghX"
                     /locus_tag="ACHU0J_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1215574.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24694..25734
                     /gene="gpr"
                     /locus_tag="ACHU0J_000762"
     CDS             24694..25734
                     /gene="gpr"
                     /locus_tag="ACHU0J_000762"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1215575.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25774..26268)
                     /locus_tag="ACHU0J_000763"
     CDS             complement(25774..26268)
                     /locus_tag="ACHU0J_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1215576.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26459..27343
                     /gene="yghA"
                     /locus_tag="ACHU0J_000764"
     CDS             26459..27343
                     /gene="yghA"
                     /locus_tag="ACHU0J_000764"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1215577.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27615..28040)
                     /gene="exbD"
                     /locus_tag="ACHU0J_000765"
     CDS             complement(27615..28040)
                     /gene="exbD"
                     /locus_tag="ACHU0J_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1215578.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28047..28781)
                     /gene="exbB"
                     /locus_tag="ACHU0J_000766"
     CDS             complement(28047..28781)
                     /gene="exbB"
                     /locus_tag="ACHU0J_000766"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1215579.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29033..30220
                     /gene="metC"
                     /locus_tag="ACHU0J_000767"
     CDS             29033..30220
                     /gene="metC"
                     /locus_tag="ACHU0J_000767"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1215580.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30360..31019
                     /gene="yghB"
                     /locus_tag="ACHU0J_000768"
     CDS             30360..31019
                     /gene="yghB"
                     /locus_tag="ACHU0J_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1215581.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31059..31958)
                     /gene="yqhC"
                     /locus_tag="ACHU0J_000769"
     CDS             complement(31059..31958)
                     /gene="yqhC"
                     /locus_tag="ACHU0J_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1215582.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32152..33315
                     /gene="yqhD"
                     /locus_tag="ACHU0J_000770"
     CDS             32152..33315
                     /gene="yqhD"
                     /locus_tag="ACHU0J_000770"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1215583.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33420..34247
                     /gene="dkgA"
                     /locus_tag="ACHU0J_000771"
     CDS             33420..34247
                     /gene="dkgA"
                     /locus_tag="ACHU0J_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1215584.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34447..35373
                     /locus_tag="ACHU0J_000772"
     CDS             34447..35373
                     /locus_tag="ACHU0J_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1215585.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35424..35681
                     /gene="yqhH"
                     /locus_tag="ACHU0J_000773"
     CDS             35424..35681
                     /gene="yqhH"
                     /locus_tag="ACHU0J_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1215586.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35724..37943)
                     /locus_tag="ACHU0J_000774"
     CDS             complement(35724..37943)
                     /locus_tag="ACHU0J_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1215587.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38054..39466)
                     /gene="ftsP"
                     /locus_tag="ACHU0J_000775"
     CDS             complement(38054..39466)
                     /gene="ftsP"
                     /locus_tag="ACHU0J_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1215588.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39541..40278)
                     /gene="plsC"
                     /locus_tag="ACHU0J_000776"
                     /gene_synonym="parF"
     CDS             complement(39541..40278)
                     /gene="plsC"
                     /locus_tag="ACHU0J_000776"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1215589.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40512..42770)
                     /gene="parC"
                     /locus_tag="ACHU0J_000777"
     CDS             complement(40512..42770)
                     /gene="parC"
                     /locus_tag="ACHU0J_000777"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1215590.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43316..43798)
                     /locus_tag="ACHU0J_000778"
     CDS             complement(43316..43798)
                     /locus_tag="ACHU0J_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1215591.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43851..44243)
                     /gene="ygiW"
                     /locus_tag="ACHU0J_000779"
     CDS             complement(43851..44243)
                     /gene="ygiW"
                     /locus_tag="ACHU0J_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1215592.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44395..45054
                     /gene="qseB"
                     /locus_tag="ACHU0J_000780"
     CDS             44395..45054
                     /gene="qseB"
                     /locus_tag="ACHU0J_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1215593.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45051..46325
                     /gene="qseC"
                     /locus_tag="ACHU0J_000781"
     CDS             45051..46325
                     /gene="qseC"
                     /locus_tag="ACHU0J_000781"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1215594.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(46371..>46617)
                     /locus_tag="ACHU0J_000782"
     CDS             complement(46371..>46617)
                     /locus_tag="ACHU0J_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR1215595.1"
                     /translation="NKYDWMLESGGNICSIPSVSGEDRILQAMIAAFFLLTPLIILIL
                     RKLFMREMFEFWLYVFSLVICLVCGWWLFWGRFIFCY"
BASE COUNT        11601 a        12355 c        11651 g        11010 t
ORIGIN      
        1 tcattttatt tttgatatcc aattttttcg tggtgtatat gtctaataaa atctttacgc
       61 attccctacc tatgcgctat gccgattttc caacgctggt tgatgctttg gactacgccg
      121 ctctgagtag agccggaatg aatttttatg acagacgttg ccaacttgaa gatcaactgg
      181 aatatcagac attaaaaacg cgtgccgaag ctggtgcgaa gcggttgtta tcgctgaacc
      241 tgaaaaaagg cgaacgcgtg gcgctgattg ccgaaacgag tagcgggttt gtagaggctt
      301 tttttgcctg tcagtatgct ggcttagtcg ccgtcccgtt ggcgattcca atgggcgtcg
      361 gtcagcggga ttcctggagc gccaaactcc agggtttact ggcaagttgc cagcccgcag
      421 ccattatcac tggtgatgag tggttgccac tggtcaatgc cgcgacgcat aacaaccccg
      481 aattacatgt tttaagccac gcttggttta aggcattacc ggaagccgat gttgcgctcc
      541 agcgtccagt tccgaacgat atcgcctacc tccagtacac ctccggcagc acccgttttc
      601 cccgtggcgt cattatcacc catcgcgaag tgatggctaa tctacgtgct ataagccacg
      661 atggcattaa attacgccct agcgaccgct gcgtctcctg gcagcctttc taccatgata
      721 tgggactggt cggctttctc ctgccccccg tcgccacgca actttcagta gattatttgc
      781 gcactcagga ttttgccatg cgtcctttgc aatggcttaa attgatcagt aaaaatcgcg
      841 gcaccgtttc cgttgcgccg ccgtttggct atgaattgtg ccaacgccgc gtgaatgaaa
      901 aagatctcgc tgaactggat ctttcctgct ggcgcgtcgc tggtattggt gctgagccga
      961 tctccgcaga acaactccat caattcgctg aatgtttccg tcaggttaac tttgacaata
     1021 aaactttcat gccgtgctac ggactggcag aaaatgcgct ggctgtcagc ttctctgatg
     1081 aagcctccgg ggttgtggtt aacgaagtgg atcgcgacat cctcgaatat cagggtaaag
     1141 ccgtcgcgcc gggtgcagag acacgcgccg tatcgacttt cgtcaactgc ggcaaagcgt
     1201 tgccggaaca tggtattgaa atccgcaatg aagcaggtat gccggtcgcg gaacgtgtgg
     1261 taggccatat ttgcatctcc ggtcccagtc tgatgagcgg ttactttggc gaccaggttt
     1321 cgcaagacga gattgccgcg acgggctggt tagacaccgg cgacctcggt tatctgctgg
     1381 acggttatct gtatgtcacc ggacgcatta aagatctgat tattattcgt ggccgtaata
     1441 tctggccgca ggatattgaa tatatagcgg aacaggaacc ggaaattcat tctggcgatg
     1501 cgattgcttt tgttaccgcc caggaaaaaa tcattttgca gatccagtgt cggatcagcg
     1561 acgaagaacg tcgcgggcag cttatccacg cgctggcggc acggatccaa agcgaatttg
     1621 gcgtgaccgc ggctatcgat ctgttgccgc cccacagtat tccccgaacg tcctccggca
     1681 agcctgcccg tgcggaagcg aaaaaacgtt atcagaaggc ttatgctgcc agtcttaatg
     1741 tgcaggaatc cctggcataa atcaaactgt cgcggtgacg ggcgctaccg ggtttatcgg
     1801 taaatatatt atcgataacc tgctcgcccg cggctttcac gttcgcgcat tgacgcgtac
     1861 tgcccgcgct cacgtcaatg ataatctcac ctgggtgcgc ggttcgctgg aagatactca
     1921 ttcacttagc gagctggttg ccggagccag cgtagtggtc cattgcgccg ggcaagtgcg
     1981 cgggcacaaa gaagagattt tcacccgctg taacgttgac ggcagcctgc gcctgatgca
     2041 agcagcaaaa gagagcggct tttgccaacg ttttctgttt atctcttcgc tggcggcgcg
     2101 ccatcccgag ctctcctggt atgcaaaatc caaatacgtc gccgagcaac gactggcagc
     2161 aatggcggac gaaattacgc tgggcgtttt tcgcccgaca gccgtgtatg gtcccggcga
     2221 taaagagtta aaaccgctgt ttgactggat gctacgcggc ctgctgccac gacttggtgc
     2281 gccagataca caactctctt ttcttcacgt caccgatttt gcgcaagcag tgggtcagtg
     2341 gttaagcgcc gaaactgtac agacgcaaac ctatgaatta tgcgatggcg tcgccggtgg
     2401 ctatgactgg caacgcgtac gacagcttgc cgccaaagcc cgttgtggtt ccgtgcgaat
     2461 ggttgacatt cctctgccgg tactcacagg ccttgcggat atcagtaccg cgttgagtcg
     2521 cctggcaggt aaagaaccta tgctgacccg ctcgaaaatt cgtgaattaa cccacgccga
     2581 ctggtcggca agtaataacc gtatttctga agatatcaat tggtttcccg ggattagcct
     2641 ggaacacgca ttacgcaacg ggctattttg attgtcttgt tttaaaggtg tgttaacgtt
     2701 tctatggtaa atcgtgaaat agtaatggat tacatccttt cctgtttaca ggatttggtc
     2761 gaaaacggtg tggaaattaa gcccgacagc gatctcgtca acgatcttgg cctggagtcg
     2821 atcaaagtga tggatctctt aatgatgctg gaagacagat tcgatatttc cattcctatt
     2881 aatattctgc tggatgtcaa aacccctgcg caattaatgg aaactctact tccatggctg
     2941 gagaataaat aatggggcta tacgataaat atgcgcgcct tgctggcgag cgtctgcaat
     3001 ttagtgacaa cgggttaaca ccgtttggca cctgtattga tgaagtgtat tccgccaccg
     3061 aaggccggat cggcaataaa aaagtgattc tggccgggac caataattat ctcggtttaa
     3121 cctttaatca tgatgccatc agcgaaggtc aggccgcgtt agcggcccaa ggcaccggca
     3181 ccaccggttc tcgtatggca aacggcagtt atgcgccgca cctggcgtta gagaaagaga
     3241 ttgccgagtt tttcaatcgc cctaccgcta tcgtttttca accggttata ccgctaattt
     3301 aggcgtcatc agcgcacttg ccgaccataa cgccgtggtg ttactggatg ccgacagcca
     3361 cgccagtatt tatgacgtct gttctctcgg tggcgcggaa attattcgtt tccgccataa
     3421 cgatgctaaa gatctggaac ggcgcatggt tcgcctcggt gaacgggcga aagaggcgat
     3481 tatcatcgtt gagggcatct acagcatgct cggtgacgtt gcgccgctgg cggaaattgt
     3541 tgatatcaaa cgtcgtctcg gtggttattt gattgtcgat gaagcgcatt cgtttggcgt
     3601 gttaggcgca acggggcgcg gactggctga agctgtcggc gttgaagatg atgtggatat
     3661 tatcgttggt actttcagta aaagtctggc ctcgattggc ggttttgctg tcgtttcgga
     3721 agcgatggaa gtgctgcgct acggcagccg cccgtatatt tttaccgcct ctccttctcc
     3781 gtcctgcatt gcgaccgtgc gctcgtcgtt aagaactatt gccacacaac cagagctgcg
     3841 gcaaaagctg atggataacg ccaaccatct gtatgacggt ctgcaaaaac tgggttatga
     3901 gttaagttca catattagcc cggtagtgcc agtaataatc ggttcgaaag aggaaggttt
     3961 acgcatctgg cgcgagctta tctcgccggg cgtgtatgtg aatcttatcc tgccgcctgc
     4021 cgcgcctgca ggcatcacgc tgctgcgctg tagcgtaaat gctgcccaca gtcgtgagca
     4081 aatcgatgcc atcattcagg catttgctac tctgaagcag taatgtgagc ccgccgtggt
     4141 gtttgcgcca cggcgggttt aatgttgtct gtcataccaa tacaccaggc tcaacgccac
     4201 caacacaaac gctaccggta aaccgagcgc ggtaacaggt gcgcttaacg caaatagcaa
     4261 tcccagatta accatgattt gatagctgat ccaggtgagt aagcccacga tgacgcctac
     4321 agcgagacgg ctacccatcc caggcgaacg cggggcacta aaggtaaacg gcacagccag
     4381 caaaatcatc gccagggtca ggatgggttg ccccagtttt tcccacagtg caaggtggta
     4441 ttcgctgctg ggttgcccgg tttcctgcaa ataatggatg taatgattaa gctgacggac
     4501 agaaaaagta ttccccggca ttgataactc ttcaagattc atactggtga aggccgattg
     4561 ccaggcaaga ttatctaatg tttccagcgt ttctttgcca ttcagccatt ttttatgatt
     4621 cacaccatgc aggacccacg ttttgtcgtc ttcaatggtg gcactgcgtg cataaatgta
     4681 ggattcaagc gagagatcgt cgcgataatg aaatatctcc acgcccaccg gctggccctg
     4741 ctcgttcagc gatttcaccg tcacaaattc attaccacgc ctggcccaca gcatattgcc
     4801 ggtaatgtca tcgtcctccc ccaacgcggt ggcggtcgat ttgatttgca acgcctgttg
     4861 ctgcaatggc gacgccaccc actcatctat cgcgcctaac gaaacagtcc acaatatccc
     4921 ggcaaccagc gccaccagtg caatacggaa gatagaaaac cccgtgctgc gaatggcggt
     4981 aagctcactg tttttcgata actgccccag tccaacaata ccacccaata acgcgataaa
     5041 cggcgaaagt tcgaccagtg tgcgcggtaa ggtcattagc accaccagca ccgcctgagt
     5101 ccagcgataa ccgcccgggc tgacaccatc cagttcgtta atcaggttga aggtggtaaa
     5161 aagcggcagc aatagccctg cagcggcggc aaaaccgaga aagagatgac ggattaaata
     5221 gcgactgaaa acattcatcg ggaaaatttc tgcagcaaag agaagtcacg tgcgaccagt
     5281 atcagcaggc ccattagcat gagtccgggc actaaccaca aaccaggaat gaggggaatc
     5341 gcgccgttag ccaccagtgt acggcagacg tcgccgccat aaaaaatggc aataaacagc
     5401 aacgtcagtg gcaataacgt tgaaaatcgc ccttgccgcg gttttacccg acttaatgaa
     5461 atggccagca gcgccatcaa caatgcggtc aggccacggc tttgtcgcca ttgcagttcg
     5521 gcatgatcgg caggaaaggc ggagcgcgcc agctccgtga ccgatttcgc tttgcgtttg
     5581 acgttcgggc tttgatccag cggattcaga tgtaattgca gattacggta gatctgttcg
     5641 ttgtcgtcac gcccctgatg atcgagaaga taggcagtgc cgttgtgcaa cataacggtc
     5701 ggtttctcgg gtgatgggtc aaccacatca accgaaccgg cgcggaaaat acgggttcga
     5761 ttggcagtag aagtataaat cagcgcgtca gtcaggcgat tattatcctg atcaaccgtc
     5821 tgcgaaagga tcattcgtcc gttatcgtta gtgttaaatt tctttgcccg caactggcga
     5881 acatccagct ccgactgcga ctgctgctcc agttgataaa tctgcgcata agcccacgga
     5941 cgaccataca tcgacagaag ggtgacaaaa atacttagcg gtatcgccag ataaagaacg
     6001 gctttgtaca agcgtcccgg actgcccccc gccgcagaga tagcggtaat ttccgagtcg
     6061 gtatacatct gccctagcgt cacgccaact gacacataca gaccaacagg taacaacatc
     6121 tccagtgcaa tcagtacttt gtagaaaacg atatccagca caacatcaag agctaatgtc
     6181 ccatttgccg cttcggtcag ataacgctgt gcggagtagc tggcgaaaat aaagatcaga
     6241 aaaccgacga taatcagcac cagacggcgc gtaccacgca tgatgtagtg ttcaaccagc
     6301 ttcatctcac taacgccctc aatcctttga gttttttatt cattgatcag tttctgcgcc
     6361 aacgcaatga catgatctac ctcttccggc gtcaggctgc cgtcctttgc ccactgaatt
     6421 tgcccggctt tattcagcac cagaatggtt gaactctgct ccggcaagcg ccaggcaacg
     6481 cgtccctgtc cattgccgtc aataacgaat tgcgcccacg gatagcggcg tttatttttt
     6541 tcgattttgc caacaacaaa atagccagta ccaaagatgg catcatcggt gttcacaatg
     6601 gtggtgggct gaaaacgatc ctgcggaaag tttgccgctt caaccgcttt aattaacagt
     6661 gagttctttt tcttcgccga cttacgcccg gcgatatact gaataatgcg gacttttccc
     6721 gctaattgtg tgctgttcca cttttggtag tgaatttcgt cgtcgctatt cagaagcagc
     6781 tcgccgcctt cctggatata aacaggggta accgttttgc cagtgacgaa attgtgtgcc
     6841 tgcacacccg gggctaagag catgataatc aacgcaataa tcaaacgaga tgacatcatt
     6901 gctccggcta tagcggaaaa cgcgaaatca gcagctctaa gcgctgacaa atatcgctat
     6961 ccagcgccaa cggtgcataa tacgactggc ggttttccat atcatccgcc accatgtcgt
     7021 cgtgtttatc tcccatcagg ccattaatgg cggcgaggaa acgggaaatc tgtaacgcct
     7081 ctttattttc aggatcaagc gatcttaagg tgtcgtaata agaaacctgc tggctgatga
     7141 tttttcgcac atcatgaaat tccctgccgg ttagtcgcgg tttggcaata atctctttca
     7201 gggtatgcat ttgcttttgc cgataggcaa taaaactgct tacgctttct aactggctat
     7261 ggcgtaatga atagttcacc aacgaccaga ccggagagct taaataaatc cgtaacaggt
     7321 tgccgtaata cgacacaata tttttcttac cgttacgaaa gccgtcctga aaacgcccca
     7381 gaaaaacggt cgttttgccg aataaaaatc cggcctgatg cttctttaaa tacagccgtt
     7441 gcgcaaaacg caggtgcttg tagcgcgctc gcatgtattt aaattgtttg cgctcatcag
     7501 cagtcagctc atcgcctttg gcttgtttgc gaactaaacg cagtaattcc tgacgatcaa
     7561 cgccattgag ccagaattgc aggcataacg cgtaattatc atgaatgatt tcggtggtac
     7621 aacttagtgc aatagtcggc ggcaaactaa cctgtgcatc aacgacatca tcttcttcaa
     7681 tcgcaacaaa tagacgctcg ctcgcctcaa gcgggaaata ttttctcatc atcaggattt
     7741 ctctccggct gcgttataaa gcaaggaatg gcgctgaaaa aatgccttta aggttggaat
     7801 taacgagagc gccacgtcca gacattgccc accgaagtaa gcaaacgctg ctcccagcac
     7861 accaaacaga tgacagaaca tgaacagcaa tacgatgtag ccgattgagg cgatggtttg
     7921 cgccacgaga aaagcgcgct gttttcccgc catcaatagc aatgactcct gcgggaagcc
     7981 cagcatcgaa atcacaattg cgcccagcat cacctgaatc aggtcatacg cttcgagata
     8041 cttaacgcca aacaccagcg aaatgagcgg cttgccgacg atcaacaccg cgagcgccac
     8101 cagaataccg attccgcccg ccagtaaacc ggacttcaca cctaacaacc acggtctggt
     8161 ggtgcgcgga tctaaacgca tcacctctgg gtaaaaactt ttacccagca aacccgctgg
     8221 cgttccggcg gcgtcgaaga atgtcatggc gattttaaat aacccggcgg cagcgggtcc
     8281 taacacgatc cccaccaaca ctgtgctgca cgagttacgc gccgaccaga tggagtgggc
     8341 aatgttggtt gaccagacaa aactccacgc gcctttaatg tgtcgggcag actcaaacag
     8401 attcaatttg aaggcgttat ggatatttct acggcgtaat tcgcgcgcgg caaaccacca
     8461 gtacatggtg ccgccaacca gattcgacac gtaccaggca ataacaaaac ccacaaaacc
     8521 aaagtcaaaa taccaggctg cgacgctccc cgctgcgcgc agaaaaggtt tcgtcgcctg
     8581 ctgtacagca atcaaatcaa agcgatctac cgcacgcaga atgccggtcg gcgtggagga
     8641 agccattgaa ggaatgagcg tgcaatacag cgccgccagc caaaagcttt ggtcatccag
     8701 acctaatgaa tgggaaagga atggcagtaa ggcaatgccg ccgacaatcg ccaccgcgcc
     8761 gctgacgata tccagcgaga aggaaaatga gacgacattg cgaaattgct gcggattatt
     8821 gttggtcaag gctggcgttc cgtactgaac caccagctgc catgtctgaa acttaataaa
     8881 atcgctgatc gacttggcgt acgattgcac aatcaccagt acaccaaaca tggcgggcgt
     8941 catcccttta ccggcacacg agagcgccaa cagccccagc aaggcgctct cgacattact
     9001 ggagcctaac caggcgctat tgcgaataat ggtgcgaaac gcgccatctg caaaccaatg
     9061 tttgatgtta aaacccgcca attcagcctg accttatcgt tgatagtaaa aaatatcccg
     9121 ccagccttaa gttaaacttc ggcggtcaga aacgatggca accagagaaa ccgccttctg
     9181 tacctgttcc agcacttcgc tgtagggcgc tctggaatca atctcaagaa tttttgtgcc
     9241 gttatagcca atcgtcgaca tgacgccgat tttgtcctgc agctcggcat actcatggtc
     9301 aggcttgcgg gagatggcag tatcaatatc aatccccaaa cgaatgatta attcaggacg
     9361 gtattgcgcc attttttggt ataaactccg ttcgcgctgc gccagaaaca tgctgatttt
     9421 cccggtcgca cgttcgacgc cgatccccgg tccgtcataa taaaagcccg aaatttcagc
     9481 ctgcgggaag cgatcgctga ccaccagaac gccactttgc gccagtcgct gaaccttgcg
     9541 tagattcgcc attcttcgca gcgagaagca gtacataatc accgccgccc atagcgccgg
     9601 agatttggtt ttcatgcttt gggttttcga ggatttggcc gccaatcgcc gttccagcca
     9661 gacgccaacc aacggcaatc gtttgatttt gtcgccgtct tcgccggaga gcagccccag
     9721 atagcggcgc tcggtttgcc agtgttgttg cagcgatttc accaggtcgg tggtcagcgt
     9781 ggatttaccg gtgccatcac acccgaccac cgcaatcagc cccggaatgt agttgggctg
     9841 cggcgcggtt gtactattaa cagtttgtgt ttgtagtgca tccattcgtt aaatcatccc
     9901 ctatccattt tggtgatcag gaaagcgcgg cgtgaattgc gcgtagtgac gcttgcataa
     9961 tttcatcggc tggttgccga ccatccagtt caaggatctt ggcgccattg aatgtcagtt
    10021 gcggcgtaac ggcgattttt tcctgtaacg ctgccagttg gtggtcaggt ttacgcgcaa
    10081 acgcggtttg ttcatcaatg ccaagacgaa tcaacaatac gggtaaataa gacgccatcc
    10141 attggtacag cttcaactcg cgctgcctta acatttttat ccaaccgtta ccgcccgtgg
    10201 tttttgccaa ttgcgggcca tcaaagcgaa aacccggcac ttcaacctgc gggtagcggt
    10261 cggtgatgag cagatagcct tgctggcttt tacacaacat tttgcgaaac ttgtacgccc
    10321 gccagcagga aagcagatag ataaccagcg cagtaatatt gccaggcgga gttaagggct
    10381 tttcgtgtac atgtgccgct ttacttcgca gataacaccc acaaggtgcg ccaataacag
    10441 ggagctgtga aatccattcg ccaattcgcc cggacgattg cccgagataa atgtgttctg
    10501 ttggcattct tgctgccagt tcatttacca ggcttgccgt gagggtcgat ttacctgaac
    10561 catcacatcc tacaatggca ataacacgca ctggcgtgga attaattatt gacatattac
    10621 gttgattcac gaaaaacccg gcagtaaata atgtattgaa tattacgtgg tccgtgctca
    10681 gaatatccgt tcaaccttat tgtgacaatg actaattgtg acattgacct gaattacgct
    10741 ttcataaaaa catattaacc aaataaatat ttttaatgga tatttaaatt aaaggatata
    10801 ttcatgcagt caataacacc tccattaatt gccgttattg gtagcgatgc ttcaggcaag
    10861 tcaacggtgt gtgaacatct tattaccgtt gtcgaaaaat atggtgctgc cgaaagagtt
    10921 catttaggaa aacaggccgg aaatgtcggt cgtgcagtga caaaattacc tttgatggga
    10981 aaatccttac ataaaacaat tgaacgaaat caggtgaaaa cagcaaaaaa attgcctgga
    11041 ccagttccgg cgctggtaat tacagcgttt gtcgcccgtc gcttactgcg ctttcgtcat
    11101 atgcttgcct gtcgtcgtcg cgggttaatt gttctaaccg accgttatcc tcaggaccaa
    11161 attcctggcg cttacgatgg tacggtgttc ccacctaacg ttgaaggtgg tcgttttgtc
    11221 tcatggctgg caagccagga acgtaaagcg tttcactgga tggcgagcca taagcctgat
    11281 ctggtcatca aactcaatgt tgaccttgaa gttgcctgtg cacgtaaacc cgaccataaa
    11341 cgggaatcgc tggcgaggaa gattgccata acgccacagt taacctttgg tggtgcacaa
    11401 ctggttgata tcgatgccaa tcagccactg gaacaggtgt tggttgatgc agaaaaagcg
    11461 attacggatt ttatgaccgc gcgtggttat cactagtcaa aaatggaaat gcccgatcgc
    11521 caggaccggg cattttcagg aaggttaaat caactgcaat gctatccagt acagcccacc
    11581 agaaagaaaa attgccgccg gtaaagtaaa cacccacgcc atcaggatgc tggttaccgt
    11641 tttacgctgt aacccaccgc cgtccaccac catcgtccct gctaccgcag acgagaggac
    11701 gtgtgttgtg gagacaggca tcccaatata actggcaagg ccgatagaca ctgccgccgt
    11761 catttgtgcc gccatgcctt gcgcatacgt catgccgcgc ttaccaatct tctcaccgat
    11821 ggtcatcgct acacgacgcc agccaatcat ggtgccaatg ccgagcgcca gtgctaccgc
    11881 catgatgatc cacaccggag cgtactcaat ggtgcttaac atatcgctgc gcagtttttt
    11941 cagcaggttc tggtcttctt tactgacgcc tggcagtttc gccagcttcg cggaggtatc
    12001 ggagatgcac agcataatgc ggcgcagctg gctgcgctga ctcacgctta acggctcgta
    12061 actttccata ttgcctggca gcatcgtttt aacgcgcgca atcgcatcaa aggtatttgc
    12121 cggatgacag tgaaactctg ttacttgcgt gccatcagtc gatgctgcag gcaatggagg
    12181 ttccatcgca atcaacttct gcggcagttc aggatgctgt tgcaggtagt gttcgaagtt
    12241 ggtaacggca tcgcgggtac gggtaatttc atagccggac gcattcatat tgacgacgaa
    12301 gccagcaggg gcaatcccca ccagtaccag cattaccagg ccgatccctt tctgcccgtc
    12361 gttcgcgccg tgcgaaaacg ccacgcccgc agcggaaaca atcagcgcaa tacgcgtcca
    12421 gaatggcggt ttacgtttgc cctttttctt tttgcgatct tccggaatgc ggtgaatacg
    12481 gtcacgcttt ttcgtcccac tccagaaacg gcgcagcagg aaaatcagac cacccgcaat
    12541 gaccaggccg acgatagggg aaacaatcag cgaggagaaa attttggtta cttcacgcag
    12601 gtttaacgca tccatcaccg atgagccggt taacagcgcg ttggttaaac cgatgccgat
    12661 aatcgcacca atcaaggtgt gcgaactgga ggccggtaaa ccaaagaacc acgttcccag
    12721 gttccagata atcgccgcca gcagcatgga aaagaccatc gccaaaccgt gagttgaccc
    12781 catattcagc aacaaatcga ttggtaacat atggacaatg gcataggcaa cgctaagtcc
    12841 gcccaataac acgccaaaaa agttaaaaaa tgccgccatc accacagcaa gttgtggctg
    12901 catggcacga gtataaataa cggttgccac cgcattcgcc gtgtcatgaa aaccattgat
    12961 tgcttcgtag aacaacacaa atgccagagc aagtaataac aaaagccccg tatatatatc
    13021 aaggccaaca aataaattta gcatatatta gattacgcca ttttgaattt acgaacggac
    13081 gcattatcag tgactttaac ggcatgggca aagtgaaata tcatttttta acgataaaaa
    13141 gagtgttatt tcttgtgtat cagacaataa gcattcattc aaaaccttta gaaataatca
    13201 aaacacaggt attccactgg ttaatatatt cacatatgaa atgaataaat attggaatat
    13261 ataaatattg aatattttga ttaatccccg tactaattat tcttcataat cagtacgggg
    13321 ttgctacaac atgtattact ttttcaccac aattaacggt tcaatatcac tctctttttt
    13381 gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc caacggtgaa
    13441 ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt gctgatagat
    13501 gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat gatggctgac
    13561 caggtcgata ttgctaccac agcgaccggc gatcggtttc actgcgtaac ctgttttcac
    13621 caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg ggaacagcga
    13681 ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg gctcaaagac
    13741 cagcacttcc gggcgcagca atacgtcgat aagacgcact tcgttttgcg gatgaccggt
    13801 acggattggc accgcagcaa actcacggtc gctaacttca cgaatctgat caaacgcggt
    13861 ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat caatcagttg
    13921 cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt caaagcccgc
    13981 ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat ctttgtcctg
    14041 catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca gttcgttaat
    14101 cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc aacgttcgag
    14161 gatcaagccc gcttcggtat gacaggaggc ggagtcggcg ttgtattcgt aaaccttcag
    14221 gccacgctca tccatgcaga aatccatacg accagtgatc atatggtgac ggcgacgctg
    14281 ccaggagaga cgcaaacgtg gccagaggat tttcggaatg tcgaacagcg ccagcaggtt
    14341 gtcatctttc agcaccttgt cggttgcgtg aagatacatc agatgcagct cgttggtggc
    14401 tttaattagc tcctgctcgg cactctcggt aatggtgtag taatgatacg gatcctgatt
    14461 gatcacctga ccgttggcct gcacataggc gttttgtagc ggatcttttt catccagcca
    14521 tttaccgtca aactggcctt tgttttccag acgcgctcca ctgattttca gcagctcgcc
    14581 tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca tccagcccag
    14641 aatggtggtg tcatcaaaag tgtctttaag ggtatagcag ccgttttcga ccaccatctc
    14701 cagctcgcgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt tctgttccgc
    14761 aatacggact ttgttgccat gcaattgggt aatgatggcg acatggccag tgtctttaaa
    14821 ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg gcgagccgtt
    14881 aggaaatgcc tgcaatggca ggatgttgtc attaaccact tctcgcagga agcgcagcga
    14941 gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtaattca gaaagagaaa
    15001 acggcgagca aattcaacgc attgccactt gtggcccata tattcgtcgt cgatatagct
    15061 acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat ctgaagagta
    15121 gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat cctggctggt
    15181 cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg cgtaattgtt
    15241 gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa tcatacacct
    15301 catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca agtgacctgc
    15361 tacactgctt caacactacc actcagaagg caactcacta tgacagacaa tacttatcag
    15421 cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa tatcaatcgc
    15481 ccgttttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacatcc attgcaactt
    15541 tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga gctactggcg
    15601 ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga tggcgatcaa
    15661 ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct gcgcgatcat
    15721 acgcataatc cgccgatccg cgtgtttgaa tctggttcga tcctgcttta tctggcggag
    15781 aaatttggct acttcctgcc gcaggatctg gcaaaacgga ctgaaacgtt gaactggctg
    15841 ttctggttac agggtgccgc gccgttcctc ggcggtggtt ttggtcactt ttaccattac
    15901 gcgccggtga aaattgagta cgccatcaac cgctttacca tggaagccaa gcgcctgctc
    15961 gacgtgctgg ataagcaact ggcgcagcat aagtttgttg cgggcgatga gtacaccatt
    16021 gcggatatgg cgatttggcc gtggtttggc aacgtggtgc tcggcggcgt gtatgatgcc
    16081 gcagagtttc tggatgcggg aagttataaa catgtgcaac gctgggcgaa agaagtgggc
    16141 gaacgtccgg cagtgaaacg tgggcgtatt gttaaccgca ccaacgggcc gctgaacgag
    16201 cagttgcatg agcgccatga cgccagtgat tttgagacaa atacagaaga taagcgtcag
    16261 gggtaagggt tggtgttcgt cgcagcaagc catccaggcc ggataaggcg ttcacgccgc
    16321 atccggcaat cgtgcacaat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    16381 gctaatcatc acgcgctggt aatgtcgtaa tccatttggc gcagtgcgtc taatgtggct
    16441 ttggcttcat cttcgtcgat gatgctcatg gcaaatccga cgtgtaccag cacccactgg
    16501 cccagcagat cggcagggtt accttcgcaa atcagggcga tattcacgtc gcgcttgata
    16561 ccacatactt caacctgcgc aagctggtga atatcttcac cgacagccag cacctggcct
    16621 ggaacgccaa tacacattgt taactccggt tatttcgatt gccggacaaa cgcgaaaaag
    16681 aacctttagc gggtgttccc tctcacccta accctctccc cggaggggcg aggggaccgt
    16741 ccgtgcgctt tttaggcgtt gtcctcagtc ttattcaact tcaatacttt tgacgatcag
    16801 cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact gcgcatcgtg
    16861 ctgatgaatc tccaccacct ggctgcaatc ccagcaccag gcctgggcgg gtttatagac
    16921 gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa aactaaaacg
    16981 gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg cggtgacgcg
    17041 cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct ggcaaagcga
    17101 caactcatgc attttcgccc ctcctgcggc caaacagcag ggcgcgacgc cctgcgtgtg
    17161 gtacatccgg attggtgact ggcaacgaaa ggatcattcg tgcgcagtca tccgtcaggc
    17221 gttggccttc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa caggagaggt
    17281 attgcgaaac accgtccagt tcgccaacgg taaaggtcat ggtgccatac ggcagttgca
    17341 gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg gggaagataa
    17401 ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg aacagcgtaa
    17461 aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc atcgaacgcc
    17521 gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg aaacctgcta
    17581 tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc gcgcagcgca
    17641 gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt taagccgatg
    17701 tgtggctcca gcgattccgg gatcacgccg accagggtca gctttttcgg aaactcaccg
    17761 gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga gattttgttg
    17821 gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg agtgttcttt
    17881 ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc gccaagcagc
    17941 tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat gtatcgttgc
    18001 tctaacgctt cgacaatccg cacgccgatg gcttcatcgg tcagcaaaat attgccgacc
    18061 cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg ccgtcggcat
    18121 ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta cgcaccactt
    18181 ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg taaggaccga
    18241 cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaacc gcctggtagt
    18301 tgctgatgat accgtcttta ataaccatcc agtgagagag cataccgcgc ggcgcttcga
    18361 ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggcttcaca aaggtggtgt
    18421 gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg atatcctgca
    18481 attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc agctgtgcca
    18541 cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc agtttgttct
    18601 gggtagattc gcgacctgcc gccagtttca ccagcatatt agccagcgga cccacttcta
    18661 ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg tcagaccaac
    18721 catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg tctttatacc
    18781 aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa tgagaagtga
    18841 tcggacgata cgaggacaga tccgcattct caatgtagcc tcccgggaac aggaagctgc
    18901 cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca cctttaccgc
    18961 gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta taaacctgct
    19021 caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc tcaaggttca
    19081 acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccacagcc aggttctgaa
    19141 tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc tggcactcca
    19201 gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt ttcatcgccg
    19261 ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc agatctttga
    19321 tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc gaaacgcctt
    19381 tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg tccacccagt
    19441 ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc gcagccagaa
    19501 tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca ctttctgccg
    19561 cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt tgcacaatca
    19621 tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc cacatggtac
    19681 cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg cgtaaatgcc
    19741 cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc tcgcctcatg
    19801 acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa tgagtacgat
    19861 gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt cccaggtcgg
    19921 gaagtaggcg taaccgccgc ccgggttgaa tgccaccaac gaataggtca gacgccaggt
    19981 tgcacaacct aacagtgcgc tcagtgctga caggaacagc atacgggaat cattacgcag
    20041 cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa tccagaacat
    20101 cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga taagctcgcc
    20161 aaagcgcagc ccgatgaaaa tcgccagcaa cacactgatg gtgttggtca gcttgacgaa
    20221 caggctcttt tcatccggac cgttgccacg cagacccgcc tgcaccagtg aaccttcaaa
    20281 gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg gcaacatttc
    20341 atagctctgc cagagcggat gcaccttgta gcccgccgag atcatcagcg accccattga
    20401 agactggtgc atggtcggca gcagcgcacc gagcgcgatg atgaagaaca tcaccttgtt
    20461 cagacgttgt agcgacacct tccagcccag acgttcaaac agtgccggag caaactccag
    20521 agccatcacg ccgatgtaga tggtcataca gaccgccgtc tcgaacagta ccgagttcac
    20581 gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca cgtcgatagt
    20641 gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg gacgcaccag
    20701 cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc agccgccaca
    20761 ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg ggaagccgcc
    20821 gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca ggagcataca
    20881 gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc cgcccagcgg
    20941 ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg gtgttgcgac
    21001 gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct ttatacaggg
    21061 tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc ggcaggtcga
    21121 gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc tctttctcgc
    21181 cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca gatttcagcg
    21241 tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt ttcgcctccg
    21301 ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact tctacgcagc
    21361 caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc tcgcacttat
    21421 gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga caggcgacca
    21481 tgcagtaacg gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg gttttcggat
    21541 cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg cagtgcatac
    21601 actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc cctgtgccgc
    21661 tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg ttcgaccagg
    21721 tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg gtgacgcaag
    21781 cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc ggaattggcg
    21841 ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc aatgccccgc
    21901 aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg agtcagcgtt
    21961 atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac cggcaaccag
    22021 cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc cctctttagc
    22081 gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa gctgatggat
    22141 gcctttatgg aagccgatac ctttttcgtt acagccatag cacgggtgac caatcgccac
    22201 cggccacaca ccgccaacat cgcagaattg cagcgttgag cagttgccgt aagtttctgg
    22261 ccctttacag ccgaggtggt acaagcacca gccttcgcgg tggccttcat caccgaactc
    22321 tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt gaatcagacg
    22381 gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggtttgc cgtaagtgat
    22441 gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg gaatattgat
    22501 aacggttttg cctggcagaa cttcttgcaa gctgactgcg ccagttgggt taactccagc
    22561 tgcggcaaca ccgccccacg cggagcagga accaatagcg ataatggctg ctgagccttc
    22621 cgccgctttg cggatgtgat ccacaatcgg ctcaccggca accatgcaat aaataccgtt
    22681 atcttttaat gggatggaac catccaccac taacacatac tgccctttgt acttctcgag
    22741 agcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca cttcgtgata
    22801 ttccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg cacgaagcag
    22861 agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg gacgctgcgg
    22921 gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca tggtggcggc
    22981 taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat ggatgagggt
    23041 gttatctcca gtcatttata gttattccgt tgcgagaacc tggcatatat tttgcctcaa
    23101 tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat ggttatcgat
    23161 atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt aattaattaa
    23221 agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat aaaaaagcgc
    23281 aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt atccagtcca
    23341 tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc ggtcttttcg
    23401 tacatcctgt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca gaattttgtc
    23461 acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc tttaccaaaa
    23521 tggttattca tcgttcacct cattcattat atatcgccat atataacgat attttaggca
    23581 aagaacgact tttgcgcacg ctattttttc atacttgttt aagacgtaat cccgtaagag
    23641 ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc aggcaagatc
    23701 ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat taacccccgg
    23761 atagatataa gcctcataaa ccttattatt gtctttcaac gccgcctcgt aaggaggcca
    23821 gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg cttcaatctt
    23881 cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg ccagttccgg
    23941 atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg taatacccac
    24001 tttacctgtc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga aatcattcat
    24061 cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat ttcccggata
    24121 acctccaacg gaacttaatc cgtcaggtgc cagggcgata taccccgcct tcgccactcg
    24181 ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca cccccacggc
    24241 tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct cgccgtgacc
    24301 atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa actctacctg
    24361 agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg tcatcccgcc
    24421 caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc cgtgaacgta
    24481 atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac gcggcatcgt
    24541 tgcattcctc aactggtgtt tttttaagca aagcataagc gcgtgttttt gcccagtttt
    24601 tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa ctagaaacat
    24661 tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa tcccgaacgt
    24721 tacgggcaga tgcagtaccg ctattgcgga aaaagtggtt tacgcctgcc cgcattatcg
    24781 ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg tgcaatcctg
    24841 cgtaaagcgt ttgatttagg cattacgcac tttgatttag ccaacaatta cgggccgcct
    24901 ccaggaagcg cagaagagaa ctttggtcgc ctgctgcgcg aggattttgc tgcttatcgc
    24961 gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc gtacggctct
    25021 ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg tatggggctt
    25081 gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat ggaagaaacc
    25141 gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg gatctcctct
    25201 tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa aattccgctt
    25261 ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag cggcctgctg
    25321 gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc tcagggattg
    25381 ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgcatcg tgaagggaat
    25441 aaagttcgtg gtctgacgcc gaaaatgctt accgaagcca acctcaacag cctgcgctta
    25501 ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt aagctggttg
    25561 ctgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga gcaactagag
    25621 gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc gcagattgat
    25681 cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa atgacctgtt
    25741 aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag tcagtcggtc
    25801 aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga cgtaccacat
    25861 cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaagt gaatggaaga
    25921 aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca ttttccccag
    25981 ccacttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat tctcataacc
    26041 ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca gcgacagcaa
    26101 cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt gaataatctc
    26161 ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt acacgggggc
    26221 aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac attcctgtcg
    26281 tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa cagcaaatca
    26341 acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc tggttccctt
    26401 atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga gaaaaacgat
    26461 gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca aacagaaaca
    26521 accgacgcca ggcatccagg cgaagatgac accgataccg gattgcggcg agaaaaccta
    26581 tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg attccggaat
    26641 aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga tcagttatct
    26701 tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat gcggacgcaa
    26761 agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc tggttcacga
    26821 agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga aacaggttgc
    26881 cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg ccattaacgt
    26941 tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag gtgcaagtgt
    27001 catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg actatgcggc
    27061 tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg cggagaaagg
    27121 tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa tttccggcgg
    27181 acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc gtgcggggca
    27241 accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga gctacgtcac
    27301 cgcagaagtg cacggcgtgt gcggcggcga gcatttaggt taaaaaaatg cccggttgtg
    27361 aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa acgccttatc
    27421 cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc tgataagcgt
    27481 agcgcatcag gcattgagca cctaatgccg gatgcgtcgt gaacgcctta tccggcctac
    27541 aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag cgtagcgcat
    27601 caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc tatcttcagg
    27661 taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc gacggttttg
    27721 tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt caacgccgta
    27781 atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc tgccttcacc
    27841 gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc aggcaagttc
    27901 accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag cagaaccaac
    27961 atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc gtcgaggttt
    28021 tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc gacacgaacc
    28081 ggatgcgcag cggcgctggc ttccagatct aggtcacggc tttgcagcaa caatacctgc
    28141 gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa tacgttatag
    28201 ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag agcttctgcg
    28261 atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc aataaagctg
    28321 ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc gccgatggtt
    28381 gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg acgctccaga
    28441 cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga cagttccagc
    28501 tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa atcagcggcg
    28561 atatcgttgg cctggtttaa ggaacgtgct tcagccagca gttgctgctc gcgcttaagg
    28621 cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca ggtgactaca
    28681 gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgta ctgatacata
    28741 ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct ccaggacgca
    28801 agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt cgttctcatt
    28861 actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca gtaaaaaagt
    28921 caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg atgcgcccgc
    28981 gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg acatggcgga
    29041 caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca ctcttggcgc
    29101 ggtaaatagc gtgattcagc gcgcttcttc gctggtattt gagagtatgg aagccaaaaa
    29161 gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg gaacgttaac
    29221 ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct gcgcgctatt
    29281 tccctgcggg gcggcagcgg ttgctaattc gattcttgct tttgtcgaac agggcgatca
    29341 tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca aaatcctcag
    29401 caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata tcgttaagca
    29461 tctgcagcca aacactaaaa tcgtgtttct ggaatcgccg ggttccatca ctatggaagt
    29521 ccacgacgtt ccggcgattg ttgccgccgt gcgcagtgtg gtgccggatg ccatcattat
    29581 gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg gcatcgatgt
    29641 ttctattcag gccgccacca aatatctggt tgggcattca gatgcgatga ttggcactgc
    29701 cgtgtgcaat gcccgttgct gggagcagct acgggaaaat gcctatctga tgggccagat
    29761 ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag gtgtgcgttt
    29821 gcgtcaacat catgaaagca gtctgaaagt ggccgaatgg ctggcagaac atccgcaagt
    29881 tgcgcgagtt aaccaccctg ccctgcctgg cagtaaagga cacgaattct ggaaacgaga
    29941 ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca atgatgaaga
    30001 gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt ggggcgggta
    30061 tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gccattcgcc cacaaggcga
    30121 gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg tcgacgatct
    30181 gattgccgat ctggacgccg gttttgcacg gattgtataa cattgccact tttggacaat
    30241 tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca aggcgtcccg
    30301 gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca ggaaagtcca
    30361 tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc gcgctggcgg
    30421 atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt ttagaaaacg
    30481 gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca ggcgcattga
    30541 ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc gcagcaagtc
    30601 tgggctgctg gctaagttat attcagggga gctggttagg gaataccaaa acggtgaaag
    30661 gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg tttgaccgcc
    30721 acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg ctgctgccaa
    30781 ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac tggttaagtg
    30841 gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg attccgttcg
    30901 ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt gccttgttaa
    30961 ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt aacgcctgac
    31021 gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg catcgcccgc
    31081 attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc acggctaaat
    31141 tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat gccgtcatgg
    31201 atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa cggcgaggtg
    31261 ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc ttctgccgcc
    31321 agttgctcga cgctcaggtt ttcggtgtat ttattctcaa ttcgtttcag cacgcggcta
    31381 atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc gcaaggtccg
    31441 gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg cgcatccagt
    31501 ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc tgataacgtg
    31561 gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc gccaatgtcc
    31621 atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc cagcggcacc
    31681 tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag caggtattca
    31741 ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc ggaaaagaga
    31801 attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc gccatacaac
    31861 aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag tttattaact
    31921 ttatccgcca gcaagcgaca aatctcttca cgtttcatat cgcgtaattt cttaggaata
    31981 atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg gagaaatagg
    32041 caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca atgaaaaaca
    32101 tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt aatgaacaac
    32161 tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc tggtttacgc
    32221 gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag cgtgaaaaaa
    32281 accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct ggaatttggc
    32341 ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact ggttcgcgaa
    32401 cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg caccaaattt
    32461 atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct gcaaacgggc
    32521 ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc agcaaccggt
    32581 tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa gcaggcgttc
    32641 cattctgcac atgttcagcc ggtatttgcc gtgctcgatc cggtttatac ctacaccctg
    32701 ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt ggaacagtat
    32761 gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat tttgctgacg
    32821 ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt gcgcgccaac
    32881 gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcactggcgt accgcaggac
    32941 tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga tcacgcgcaa
    33001 acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa gcgcgctaag
    33061 ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga tgagcgtatt
    33121 gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc gacccacctc
    33181 tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact ggaagagcac
    33241 ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg ccgtatatac
    33301 gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt tcgtccagac
    33361 ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag gaggaacgta
    33421 tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag ctgggactgg
    33481 gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg ttagaagtgg
    33541 gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc ggcaaagccc
    33601 tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg tggaacgacg
    33661 accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag cttgattata
    33721 tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc gaagcatgga
    33781 aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg tgcaacttcc
    33841 agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg ataaaccaga
    33901 tcgaacttca tccgctgatg caacaacgcc agctacacgc ttggaacgcg acacacaaaa
    33961 tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc gatcagaaag
    34021 tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt atccgctggc
    34081 atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt attgccgaaa
    34141 actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcaaaatt gcaaaactcg
    34201 atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacgt gcaaattctc
    34261 ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc cctcgcgtcc
    34321 cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca tcatgcagag
    34381 ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg gagaggtgaa
    34441 aaacagatga aaataatact tctgttttta gcagccctgg caagttttac cgtacacgcg
    34501 cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa ctataaatca
    34561 gatgccagta cgccttattt cggtgaaacc ggagagcggg cgataacttc agcgcgtatt
    34621 caacaggcgc ttaccctgaa cgacaatctt acactaccgg gcaatattgg ctggctggat
    34681 tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag cgtagcgata
    34741 acccaaactg acgtcgatca tgccgatgcc gttgtgcgct ttcgtatctt taaagatgat
    34801 aaagaaaaga ccacacagac actgaaaatg gtggcggaaa atggtcgttg ggtcattgat
    34861 gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa tgaaaaaacg
    34921 ctggccgctt tagcttcgtt gcagaaagaa cagccagaat cctttgttgc tgaactcttt
    34981 gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc ttaccgccag
    35041 gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga tgaagatatg
    35101 caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga gtcgctattt
    35161 tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg cattcatgtt
    35221 cgttttacgc tgaccaatgg caacaatgaa gagcaagagc tggttttaca gcggcgcgaa
    35281 ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact taagcagatt
    35341 gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa ttagccggaa
    35401 caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct gttgttctgg
    35461 caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcaactg gcgagcaatg
    35521 tacaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca ctacgcccac
    35581 aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat gcacaggact
    35641 attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat ccccggcagc
    35701 atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg tttcttcgca
    35761 ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc aggcgtctga
    35821 cgctgagtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg gttctggcga
    35881 cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg acggctgcca
    35941 atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca gtttgccgga
    36001 tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt cggtacgaag
    36061 acgccttcgc tcttataacc aatcttcgcc agcggttttt tcccggtgta atacatggtg
    36121 gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg gaaacgatgc
    36181 tttttcagcc acagcgccaa attcaccata tcttcatcac gcgtaccggg gtgcgcagag
    36241 atgaaatacg ggatcagata ctgctcttta cctgcctgtt tcgagtaggt atcgaacagc
    36301 tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgacaa cggcccttct
    36361 tcggtatgtt ccggagcaat cttcagataa ccgccgacgt gatgggtcgc caactctttg
    36421 atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat aaggattttt
    36481 ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc gtggttagtg
    36541 tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt ttgttcagcg
    36601 cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag atcggaaatc
    36661 acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat gatcgaatct
    36721 tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa agaacagccg
    36781 ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc cggaatacgg
    36841 gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac gctgtccatc
    36901 tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc accgtgtttt
    36961 tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg cgaagcatgg
    37021 gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaatac gtaggttttt
    37081 tcccacggtt tctggcgcgg tggctgcacg attacagctt tcgcttcctg ctttttcggc
    37141 gcaacaggtt tgttatccgc acacggcaaa tcttcgccat acggatgcgg gattgggtcg
    37201 attttcccta gggtatcaag acgggtggaa tccacaccgc tccagccagg cagcgcctct
    37261 tttacgataa ttgcggtatt acgcacatcg cggatttcac taatcggctc gcccatcgcc
    37321 agacgatgcg ctacttccac cagcggacgt tcaccgttac caaacatcag catgtcggct
    37381 ttcgaatcta ccagaacgga acggcgcacg gtatcggacc agtaatcata atgcgcggta
    37441 cggcgcaggc tagcctcaat accgccgagg atcaccggca catctttcca cgcctcttta
    37501 caacgctggg tataaaccag cgtggcgcga tccggtcgct tacccgcgac attatctggc
    37561 gtgtaggcat catcatgacg taaacggcga tcggcggtat agcggttgat catcgaatcc
    37621 atgttgccag cagtaacacc aaaaaacaga ttcggtttac ccagacgcat aaagtcgtct
    37681 ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgcgc ttccagcata
    37741 cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc agtaaccaaa
    37801 atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat cggcaaaaac
    37861 ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc gcgatccggt
    37921 tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata atcaaaggcc
    37981 ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg ccctcctcgg
    38041 gcgagtatga agattacggt accggattga ccaacagttg cccaatcgag ccacggtccg
    38101 ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa ggctgaccga
    38161 aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag cctctgtctt
    38221 ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct tcaatatgga
    38281 acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc tgcgcggtga
    38341 catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca cccagactga
    38401 tatcgcgact gcgaattggc gaaccagcca tgatttcagt tggcagcaag cgcatcggaa
    38461 gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc aaggtagaaa
    38521 ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc gccgcttcac
    38581 cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg cggcgctcgc
    38641 ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc tgatcgccag
    38701 aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga gagttcgacg
    38761 cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg ctttgtacac
    38821 cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg tattctggcg
    38881 taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc acaccataat
    38941 ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt ccggcaaggc
    39001 cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac agagtagctg
    39061 cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc atccgtgccg
    39121 gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag acattttctg
    39181 tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg atagtcggcc
    39241 ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta aatgaccagt
    39301 gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt agcggcggaa
    39361 cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg cctgcacaaa
    39421 gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt gctccatgta
    39481 aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca gattgccgac
    39541 ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg cgattttttg
    39601 ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc catactgact
    39661 gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc gattaagatt
    39721 aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg ccgcaattgc
    39781 cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc gggttccttc
    39841 cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt ccgcaatggt
    39901 gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta accagtacaa
    39961 ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg tcggcggttg
    40021 cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga tatagatagc
    40081 attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc cgaacagtgg
    40141 cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac gcgggctgaa
    40201 aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg taataataag
    40261 acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt gtacgtcacc
    40321 tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt gttaatcgca
    40381 acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt tgaatacgct
    40441 gccggatgac gacttaacgt ttcatccggc gttccttgct agcgggagga aacagcgccc
    40501 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    40561 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    40621 actttctgta gctcttccgg acgcagttta attttgcgtt tcccaacatg aatggtcagc
    40681 gtgctttgcg gcggcagaac gtacagttgc gccagaccat cctcgccacg cgcggcttct
    40741 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    40801 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    40861 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttaccc
    40921 gcacggttac gcgctaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    40981 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    41041 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    41101 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttactctta
    41161 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    41221 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    41281 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    41341 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    41401 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    41461 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    41521 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    41581 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    41641 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    41701 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    41761 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    41821 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    41881 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    41941 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    42001 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    42061 ccacgtccgt tctcgtagat tttacgaatc tcggcgcgcg aagtgataat ttccgcttca
    42121 gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtagt tttcggctgg
    42181 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    42241 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgggcaggt
    42301 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    42361 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    42421 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    42481 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    42541 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    42601 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttcaga
    42661 ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    42721 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    42781 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    42841 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    42901 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    42961 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    43021 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    43081 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    43141 gcggcttgct catggatatc cccgagacgg cttacggcgc accggataat aaatttcgcg
    43201 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtgacatt
    43261 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    43321 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    43381 tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    43441 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    43501 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    43561 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcaggggcgt
    43621 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    43681 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    43741 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    43801 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    43861 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    43921 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtgatcgata
    43981 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    44041 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctaca
    44101 gtcgttacgc tgccgttcgg cccctgaaat cctccggcct gactttgcgt taccgatggg
    44161 ccagaaaaac cgccctgctc tgctgccatc accggtgcac tgcacagggc cattactgcg
    44221 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    44281 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttaatg ctgtacagag
    44341 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    44401 attttactga tagaagatga catgctgatt ggtgacggca tcaaaactgg ccttagtaaa
    44461 atgggtttta gtgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    44521 gcaccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    44581 ttgcgcgaat ggcgagagaa aggtcagcgt gagccggtac tgaacctgac cgcgcgtgat
    44641 gcgttagcgg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    44701 tttgcgttga tagaagtcgc cgccaggctg gaggctctga tgcgccgaac caacggccag
    44761 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    44821 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    44881 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    44941 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    45001 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    45061 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    45121 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    45181 tcgacaccca actgatgctg tttgccaaac ggttaagtac gctcgatctc aacgaaatca
    45241 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    45301 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaat gatggcgata
    45361 acggagaaga tattccctat agctatcaac gggaaggttt tgctgacggg caactggtcg
    45421 gtgaagacga tccttggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    45481 ttgttggcca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    45541 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctactgggtc
    45601 gtgaactcgc gccgctgaac aaactggcgc tggcactacg tatgcgtgac cctgactcgg
    45661 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg gccactggtt gagtcgctaa
    45721 atcaactgtt cgcccgcaca catgcgatga tggttcgaga acgacgcttt acctccgatg
    45781 cagctcacga actacgtagc ccgttaacag cgctgaaagt gcaaaccgaa gttgcgcagc
    45841 tctctgacga tgatccgcag gcgcggaaaa aagcactgct ccaattacat tccgggatcg
    45901 atcgcgctac tcgtctggtt gatcaactgc tcacgctatc gcggctggac tcactggata
    45961 accttcagga cgtcgcggag atcccgcttg aagatctcct acaatcgtcg gtgatggata
    46021 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    46081 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    46141 gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat cgcccacccg
    46201 gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc gccaagctgc
    46261 atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg aaggtaagct
    46321 ggtaacttct ctgtctatga gagccgttaa aacgactctc atagatttta ctaatagcaa
    46381 aatataaacc gtccccaaaa aagccaccaa ccacaaacca gacaaataac gagcgagaat
    46441 acatatagcc aaaactcgaa catttcccgc ataaagagtt ttcttaagat aagaataata
    46501 agtggcgtaa gaagaaaaaa tgctgcaatc attgcttgca atatacgatc ttcgcctgaa
    46561 acggaaggta tactgcaaat attgcctccc gactcaagca tccagtcata cttattg
//
LOCUS       DAZPDE010000016        44663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPDE010000016 DAZPDE010000000
VERSION     DAZPDE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.016"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..870)
                     /gene="rpnA"
                     /locus_tag="ACHU0J_000783"
     CDS             complement(<1..870)
                     /gene="rpnA"
                     /locus_tag="ACHU0J_000783"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR1215596.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
     gene            complement(1073..1309)
                     /gene="feoC"
                     /locus_tag="ACHU0J_000784"
     CDS             complement(1073..1309)
                     /gene="feoC"
                     /locus_tag="ACHU0J_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1215597.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1309..3630)
                     /gene="feoB"
                     /locus_tag="ACHU0J_000785"
     CDS             complement(1309..3630)
                     /gene="feoB"
                     /locus_tag="ACHU0J_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1215598.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3647..3874)
                     /gene="feoA"
                     /locus_tag="ACHU0J_000786"
     CDS             complement(3647..3874)
                     /gene="feoA"
                     /locus_tag="ACHU0J_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1215599.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4312..6633)
                     /locus_tag="ACHU0J_000787"
     CDS             complement(4312..6633)
                     /locus_tag="ACHU0J_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1215600.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6730..7206)
                     /gene="greB"
                     /locus_tag="ACHU0J_000788"
     CDS             complement(6730..7206)
                     /gene="greB"
                     /locus_tag="ACHU0J_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1215601.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7434..8153
                     /gene="ompR"
                     /locus_tag="ACHU0J_000789"
     CDS             7434..8153
                     /gene="ompR"
                     /locus_tag="ACHU0J_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1215602.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8150..9502
                     /gene="envZ"
                     /locus_tag="ACHU0J_000790"
     CDS             8150..9502
                     /gene="envZ"
                     /locus_tag="ACHU0J_000790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1215603.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9578..11200)
                     /gene="pckA"
                     /locus_tag="ACHU0J_000791"
     CDS             complement(9578..11200)
                     /gene="pckA"
                     /locus_tag="ACHU0J_000791"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1215604.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11579..13337
                     /locus_tag="ACHU0J_000792"
                     /pseudo
     CDS             11579..13337
                     /locus_tag="ACHU0J_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13481..14350)
                     /gene="hslO"
                     /locus_tag="ACHU0J_000793"
     CDS             complement(13481..14350)
                     /gene="hslO"
                     /locus_tag="ACHU0J_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1215605.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14375..14776)
                     /gene="hslR"
                     /locus_tag="ACHU0J_000794"
     CDS             complement(14375..14776)
                     /gene="hslR"
                     /locus_tag="ACHU0J_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1215606.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14787..15455)
                     /gene="yrfG"
                     /locus_tag="ACHU0J_000795"
     CDS             complement(14787..15455)
                     /gene="yrfG"
                     /locus_tag="ACHU0J_000795"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1215607.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15520..17655)
                     /gene="igaA"
                     /locus_tag="ACHU0J_000796"
     CDS             complement(15520..17655)
                     /gene="igaA"
                     /locus_tag="ACHU0J_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1215608.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17975..18535
                     /gene="nudE"
                     /locus_tag="ACHU0J_000797"
     CDS             17975..18535
                     /gene="nudE"
                     /locus_tag="ACHU0J_000797"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1215609.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18700..21252)
                     /gene="mrcA"
                     /locus_tag="ACHU0J_000798"
     CDS             complement(18700..21252)
                     /gene="mrcA"
                     /locus_tag="ACHU0J_000798"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1215610.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21372..22151
                     /gene="hofM"
                     /locus_tag="ACHU0J_000799"
     CDS             21372..22151
                     /gene="hofM"
                     /locus_tag="ACHU0J_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1215611.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22151..22690
                     /gene="hofN"
                     /locus_tag="ACHU0J_000800"
     CDS             22151..22690
                     /gene="hofN"
                     /locus_tag="ACHU0J_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1215612.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22674..23114
                     /gene="hofO"
                     /locus_tag="ACHU0J_000801"
     CDS             22674..23114
                     /gene="hofO"
                     /locus_tag="ACHU0J_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1215613.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23104..23510
                     /gene="hofP"
                     /locus_tag="ACHU0J_000802"
                     /pseudo
     CDS             23104..23510
                     /gene="hofP"
                     /locus_tag="ACHU0J_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23422..24660
                     /gene="hofQ"
                     /locus_tag="ACHU0J_000803"
     CDS             23422..24660
                     /gene="hofQ"
                     /locus_tag="ACHU0J_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1215614.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25061..25582
                     /gene="aroK"
                     /locus_tag="ACHU0J_000804"
     CDS             25061..25582
                     /gene="aroK"
                     /locus_tag="ACHU0J_000804"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1215615.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25639..26727
                     /gene="aroB"
                     /locus_tag="ACHU0J_000805"
     CDS             25639..26727
                     /gene="aroB"
                     /locus_tag="ACHU0J_000805"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1215616.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26819..28111
                     /gene="damX"
                     /locus_tag="ACHU0J_000806"
     CDS             26819..28111
                     /gene="damX"
                     /locus_tag="ACHU0J_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1215617.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28218..29054
                     /gene="dam"
                     /locus_tag="ACHU0J_000807"
     CDS             28218..29054
                     /gene="dam"
                     /locus_tag="ACHU0J_000807"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1215618.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29072..29749
                     /gene="rpe"
                     /locus_tag="ACHU0J_000808"
     CDS             29072..29749
                     /gene="rpe"
                     /locus_tag="ACHU0J_000808"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1215619.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29742..30500
                     /gene="gph"
                     /locus_tag="ACHU0J_000809"
     CDS             29742..30500
                     /gene="gph"
                     /locus_tag="ACHU0J_000809"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1215620.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30493..31497
                     /gene="trpS"
                     /locus_tag="ACHU0J_000810"
     CDS             30493..31497
                     /gene="trpS"
                     /locus_tag="ACHU0J_000810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1215621.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31767..32672
                     /gene="yhfZ"
                     /locus_tag="ACHU0J_000811"
     CDS             31767..32672
                     /gene="yhfZ"
                     /locus_tag="ACHU0J_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1215622.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32689..33051
                     /locus_tag="ACHU0J_000812"
     CDS             32689..33051
                     /locus_tag="ACHU0J_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1215623.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33135..34298
                     /locus_tag="ACHU0J_000813"
     CDS             33135..34298
                     /locus_tag="ACHU0J_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR1215624.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34299..35525
                     /locus_tag="ACHU0J_000814"
     CDS             34299..35525
                     /locus_tag="ACHU0J_000814"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1215625.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35522..36400
                     /locus_tag="ACHU0J_000815"
     CDS             35522..36400
                     /locus_tag="ACHU0J_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1215626.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36411..36764
                     /locus_tag="ACHU0J_000816"
     CDS             36411..36764
                     /locus_tag="ACHU0J_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1215627.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36776..38080
                     /locus_tag="ACHU0J_000817"
     CDS             36776..38080
                     /locus_tag="ACHU0J_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1215628.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38092..39177
                     /locus_tag="ACHU0J_000818"
     CDS             38092..39177
                     /locus_tag="ACHU0J_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1215629.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39337..40068)
                     /gene="frlR"
                     /locus_tag="ACHU0J_000819"
                     /gene_synonym="yhfR"
     CDS             complement(39337..40068)
                     /gene="frlR"
                     /locus_tag="ACHU0J_000819"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1215630.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40168..40953)
                     /gene="frlD"
                     /locus_tag="ACHU0J_000820"
     CDS             complement(40168..40953)
                     /gene="frlD"
                     /locus_tag="ACHU0J_000820"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1215631.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40950..41780)
                     /gene="frlC"
                     /locus_tag="ACHU0J_000821"
     CDS             complement(40950..41780)
                     /gene="frlC"
                     /locus_tag="ACHU0J_000821"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR1215632.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41830..42852)
                     /gene="frlB"
                     /locus_tag="ACHU0J_000822"
     CDS             complement(41830..42852)
                     /gene="frlB"
                     /locus_tag="ACHU0J_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1215633.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42873..44210)
                     /gene="frlA"
                     /locus_tag="ACHU0J_000823"
                     /pseudo
     CDS             complement(42873..44210)
                     /gene="frlA"
                     /locus_tag="ACHU0J_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44505..>44663)
                     /locus_tag="ACHU0J_000824"
     CDS             complement(44505..>44663)
                     /locus_tag="ACHU0J_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1215634.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
BASE COUNT        10408 a        11337 c        12221 g        10697 t
ORIGIN      
        1 cgccgcagcc aactcttctt ctgacagccc ggtaaatcgc atgatgtctg caagagggac
       61 tccggattca agcattattt tggctatatg cagggctttg gattgttccc cctcctgccg
      121 caatctttcc gcaatagtca ttaaactctc cttgtgtttc ggtgaacgtt cggcaacgcc
      181 gtcgataaaa tcgttaaaac gtacagcgtc gccagtttgc agtatgtaat taaacagccc
      241 tttgatttgt ctgtcattag cgtatccact acttaataag caggccattt gctctaccag
      301 ccccatcaga tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg
      361 gtgctgcatg atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc
      421 ataaagctgc cttgccagtt tgggatcggc gaaacaatcc agccaacaca gcgaataggg
      481 ataggggctt tcaataccgt ggtaaaacaa caatggcacc accatcggca acgttttgta
      541 tccagcatca aggtgatttt gcattgcggc aatagcgtaa cgcatcatgc gaaatgcgat
      601 cagtttgttt gaggtgcttt gatgttcaat cagacaatag atgtatcctg gtccttgttc
      661 cgttttcacc gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct
      721 cctcgactcc agcttgaggg ttttcatatc acagagcgcg tgaatgggtg ccggtaaatg
      781 aaacgcaaga aaatcgcgtg ccgtctccgg ttggcgtaaa aagagtttga acagcgcatc
      841 atgtggggtg gaactctgct ttttactcat ggcgttcctt cacctgttgt ttgaatgccg
      901 cgactctacg cgccactcca catctgtgca ttagcgagtt ttcatccctg cgagacgctt
      961 tcagaggaac ttacgttgcg ctgcaagaat tgcattttta tggaaagccg cacgcaaaaa
     1021 agcccggatg caaacgcata ccgggctttt caaaacaggc gatggagtat ggttaacgca
     1081 gcgcccacca ctcgcgcaga caggctttgc cttccgggca gcttttacaa ctgccagaga
     1141 ggcagccgtc aggttcttcc tgaatccgca cggctttgcc catgctttcc agctgttgca
     1201 gcatggcgtt aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca
     1261 tacggccccg taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc
     1321 accggtggtg ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat
     1381 atccacccgg ctacgcgcgc ggcgcagcag accgatagct acgatgttaa acaggattac
     1441 cgccagaatg cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa
     1501 caatgttgcc agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc
     1561 acggcttgat tcacgggcga tggctcccat caccgagata catggtacat acagcaggac
     1621 gaaaatcagg tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat
     1681 cgccccggta cccatttcac cgttgccttt gctagcttca atggggttca tcagtacgct
     1741 aaggctgaag gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc
     1801 accgaggttg aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag
     1861 ggtgttgagc gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt
     1921 tgcctgccag ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct
     1981 gacggaagcc agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct
     2041 gttgaaagcg ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa
     2101 gcccttcaga cgctgccagg tctggataat caggctttta acgtgtggta catggtagac
     2161 cggcagctcc atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag
     2221 gccagtcagc accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc
     2281 gttctgcccg aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga
     2341 cataaacggt gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc
     2401 gcccattacc gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc
     2461 cggcaagccc agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc
     2521 ggagtcctca aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag
     2581 cacggtgtta ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa
     2641 gtggagcgtg tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtt
     2701 aaacagcggc tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac
     2761 aaagaggaaa atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt
     2821 gaaacggctg ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagcaatgca
     2881 ctggtaacgc gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc
     2941 gagggtggca tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc
     3001 ttccagcatt tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac
     3061 ttttgccagt gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt
     3121 ctcgttagct ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt
     3181 tgaaaccagc gggatcaccg ggcagccaag acgtgccgat agagcatcaa tttcaatacg
     3241 aatattttgc ttctcggcaa tgtcgagcat gttcagcgcc acgatgcagg gaatgccgag
     3301 ttccagcagt tgcagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt
     3361 aatcagcagg tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga
     3421 ggtctgcgat gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg
     3481 atgatcggtg gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc
     3541 tacacgctga cgtgcgccag tgagctggtt aaataaggtt gtcttgccag aatttggatt
     3601 accaattaag ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg
     3661 cttccacttc taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt
     3721 cgatatgaat ggggtcgccg agtggagcga cgcgcaccac atcaaaagag gagccaggta
     3781 acatgcctag agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag
     3841 tgattttcca cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact
     3901 ggataactac ctcatggggc gtttgaccgc gacatgagac cagatgattt gccaacaaag
     3961 gagagattaa ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta
     4021 aaatgtgtgc gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt
     4081 tatatcgcag cgaaaatata attcgaatag tgagttaccc ggactggcta atgtttaatt
     4141 aaggtttcag ttgttaatga agtgataaat attaacggag tatattgtga gtttggctgt
     4201 ggaataaaag tgccggagat attctccggc acttttatgt caggcaaagc gttcgttgcc
     4261 agatgcggcg tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt
     4321 tttacccatc gctgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc
     4381 acgaccgcgt ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg
     4441 acgagagttg gtttcgccag gctgctcgtc cagacgcata gtcagggcga tacgtttacg
     4501 ctgaagatcc acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg
     4561 cggatcttcc acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac
     4621 gccaatatcg acaaacgcgc caaagttggt gacgttggtc actgcgcctt cgaggatcat
     4681 acccggttgc aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc
     4741 cggacgcgga tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac
     4801 accgaatttt tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc
     4861 catcagatct ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc
     4921 ttccggatga acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc
     4981 cgcgcactgc tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt
     5041 ctggaactgg ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag
     5101 gcccgccacg cgggttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt
     5161 tacgcagtct tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg
     5221 ctgatactga cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc
     5281 ctgcaaacgg cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc
     5341 ctgcgctgcc agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt
     5401 ctgtgcggtc actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg
     5461 ggaagctgta ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc
     5521 aacggtcatc gctgcttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac
     5581 cagtttgcca gtggcatcga ccaccgccac ttttaccccg gtacgcagac ccggatcgag
     5641 gcccatcgtt gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc
     5701 aaagacgttg attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc
     5761 cagatgcatc agcaccttga tgcgccaggt ccagctcact acgcttttgc gccagctatc
     5821 cgccggggca ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct
     5881 ctctttgggc ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc
     5941 gttacgccca cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg
     6001 atgatcgaaa tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga
     6061 aaccaaatgc gcgttcttcc acagataatc acgcactttc gccagcagcg tggcatcttc
     6121 ggcaaaccgt tccatcagga tatagcgcgc gccatccagc gcggctttgg tatctgccac
     6181 gcctttatcg gcatcaacat attgtgcagc ggcgacttct ggcgtgtgtg acggatcgct
     6241 ccacagcagg tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg
     6301 gcgtttaggt ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt
     6361 gatggccttc gccagatcat cggtgagttt gccttgctcg gaaatggatt tgaggatcgc
     6421 ctgacgtctc tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg
     6481 cgtgtcatcc agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt
     6541 cccttcgtca agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc
     6601 cgcaataatg cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa
     6661 aatcagggga tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa
     6721 gactcttcct tacggtttca cgtactcgat agcattaaca taccagttcg cttccccggc
     6781 aggggtattc accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg
     6841 ggaatcgata gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa
     6901 acggtgagtc acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc
     6961 ttcctgctga ggggagtaat cgacgatttt gagattttcc aggcatttag tgagatagcg
     7021 cacgcgacgg tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct
     7081 gcggtcgccc agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg
     7141 ccagagataa ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt
     7201 tttcatctcg ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc
     7261 atatcaacag aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat
     7321 atgctttgta acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc
     7381 ttttttaaga atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag
     7441 agaactacaa gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt
     7501 atctcaccga acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc
     7561 tgctgactcg tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg
     7621 gcttgtcgat ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga
     7681 cggcgaaagg ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca
     7741 ttccaaaacc gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc
     7801 aggcgaacga actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt
     7861 tcaaacttaa cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca
     7921 gcggtgagtt tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg
     7981 ataagctgat gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg
     8041 tgcagatttc gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc
     8101 agaccgtttg gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt
     8161 gcgcttctcg ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt
     8221 cgccagcctg gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca
     8281 gcagtttaat aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact
     8341 ggaggacggc acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct
     8401 ggggatctct ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca
     8461 ctatgaattc ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt
     8521 tgaggtcaac aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg
     8581 ggtacgcgtg ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac
     8641 gctggcgatt atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg
     8701 accgttggtc gatctcgaac acgcagcctt gcaggttggt aaagggatta ttccgccgcc
     8761 gctgcgtgaa tatggcgctt cggaggtgcg ttccgttacc cgtgccttta accatatggc
     8821 ggctggtgtt aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga
     8881 cttgcgcacg ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg
     8941 ctatctggca gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt
     9001 tatcgactac ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt
     9061 gctcggtgag gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta
     9121 ccccggcagc attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat
     9181 ggtggtcaac gccgcccgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc
     9241 gaatcgcgcc tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa
     9301 gcacctgttc cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt
     9361 agggctggca attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac
     9421 cagcgagcgg ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca
     9481 gggcacgaca aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct
     9541 ataagatact ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta
     9601 ccagcgcggc acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg
     9661 ccagggtttc ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga
     9721 gaatcttcgt gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag
     9781 tgaaggtttc tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat
     9841 ctttaatcga gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg
     9901 cgcccgccgc ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca
     9961 ggaacgccgc gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag
    10021 taccggccag tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca
    10081 ggcgagaaac cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg
    10141 cgtggcccgc tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa
    10201 cgcgggtgtt ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg
    10261 tgacgttttc cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt
    10321 tcgacagctt gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt
    10381 cccagccgtg ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt
    10441 taccggtgcc ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg
    10501 agcagtgcat agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc
    10561 ctttcttcat ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt
    10621 taaacgccac gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact
    10681 tcgcgccgtt cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc
    10741 gaataaacat gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg
    10801 aaagacgagt atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg
    10861 aaagctgcct ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt
    10921 tgtcgttctt acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac
    10981 ggacgatata cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg
    11041 cacccagatt agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct
    11101 gatacagcag gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag
    11161 cctcgagttc ttgcgggggc aaaccattgt taacgcgcat ttcactgctc cttagccaat
    11221 atgtattgcc tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat
    11281 ttacgtatct ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg
    11341 ttgctattat cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct
    11401 tggtttatat ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa
    11461 ttcaaccggt aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag
    11521 caaaatacta ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat
    11581 ggataacgtt gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca
    11641 ggggctggtt tgctacctgc ttatcgcctg gcttgccgga aaaaatcaca gttggattgt
    11701 ttatggcgtg cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt
    11761 taaacagaag cgcctttggg gatggctggc gctggtgttt attgccacga cagggatggc
    11821 tggcgctggt gtttattgcc acgacaggga tgagcggttg gctgaagtgg caaactgatg
    11881 gcatgaaccc ctggagagct gaaaaggcga tttgggattt tggctgctat ctgctgttga
    11941 tggcaatgtt gttgctaccg tggatacaac aaagccttcg tatccgcaat ggcagtagcc
    12001 gctatagcta cttttaccag tcagtatggc ataacgtact tatattactg gtgatttttc
    12061 tctccaatgg cctgacgtgg ctggtgcttt tactgtgggg tgagttgttt aaacttgttg
    12121 gcatcacgtt tttcaatacg ctcttttttg caaccgactg gtttatgtat cttacatttg
    12181 gtctggttac cgcactggcg gtgatcctcg cgcgtacaca gtcacgttta atcgactcta
    12241 ttcaaaagtt gttcacgcta atcgccacgg ggttgctgcc gttagtatca ttgctaaccc
    12301 tgatgtttat catcaccctg ccgtttacgg gcctgagcgc gatttctcgc cacatctccg
    12361 ccgccgggtt gctgttaacg ctggcctttt tgcaattgat cttaatggct attgtccgcg
    12421 atccgcaaaa agcgtcactt ccctggacgg ggccgttgcg ttgcctgatt aaaaccgctt
    12481 tgctggttgc tccgctgtat gtgttcgtcg ccacctgggg gttatggctg cgggtcgctc
    12541 agtacggctg gactgtcgac cgcttgcagg gcgcgctggc ggagctggtg ttactggtgt
    12601 ggtcgctggg gtattttgtc agtatcgtct ggcgtaatgg gcaaaatccc cttgttcttc
    12661 agggcaaagt gaaccttgcg gtttcgttat tggtgttggt gatactggtg cttcttaatt
    12721 cgccggtgct ggacagtatg cgtattagcg tgaacagtca tatggcgcgt tatcagagcg
    12781 gcaaaaacac gccagaccag gtaactatct acatgctcga acagagcggt cgctatggac
    12841 gtgcggcgct tgagtcgctg aaaagcgatg ccgggtttat gaaagacccg aaacgcgcgc
    12901 gggatctgct gatggcgtta gatggagagc aacatcttca ggaacaggta tcggaaaaag
    12961 tattcgccga aaatgtgtta attgcccctg gttctgttaa accggatgcg acattctggt
    13021 cggccttaat ccaggatcgc tataacgtga tgacctgtat tgaaaaagac gcctgcgtac
    13081 tggtcgagca agatctgaat agtgatggtc aggcggagcg gatcctgttt gcttttaatg
    13141 atgacagagt cattgtctat ggctttgact cagacagaaa agaatgggac gcgcttgata
    13201 tgagtttact tccgaacgaa ataacgaaag aaaaattact cacagctgcg aaggatggaa
    13261 aactggggac gaggccgaaa gcgtggcgcg atctgacggt agatggtgaa acgctggaaa
    13321 taaacttaag taagtgacaa cgcttttatc ggatacgacg ctgtatctga ccaaaataat
    13381 ttgcgattta tttcataggc gctggtaagc gcagcacatc aggccattaa gtcgtaaaat
    13441 ccggcagagc cctctctgcc ggacatactc attaatgaac ttacggatct gccggagacg
    13501 cgttgttgcg gatttcagca atatccatcg cattgaacag atagtggtta ccgcagtaat
    13561 cacaatgcat gtcaatttcg ccatcttccg ccaggatgct atcaacttct tcatcaggca
    13621 gcgttttcag cgcatcggcg caacgttcac gcgagcaggt gcatttgaac tccacatcct
    13681 gcggatcgta aaccgtcacc tcttcttcgt gatacaaacg ccacaacact tcgtttgccg
    13741 gtaaggtcag cagttcttcg gttttgatgg tttcggttag cgtcgccagg tggtcaaagt
    13801 cgtcctgttg ggtattttgc gcaggcatta cctgcaacaa cataccgcct gcagccggtt
    13861 tgccgtctac gtcgccggtg cgaataaaca ggcgcgtcgg cagctgttca gaacgcataa
    13921 agtaatcttc taggcaggcc gccagggtat caccttccag accaactacg ccctgatagc
    13981 gttcgccttc gctcggggta atggtgatca ccacgtaacc attgccgacc agcgttttca
    14041 ggtcggcatt ttctggaatt tcgccctgca cgcgcgccac accgcgcatt tgctggttat
    14101 tgttaccgtt aataaccgcc agattcatcg gaccgtcgcc ctgcagctgt acggtgatat
    14161 caccatcaaa cttcagcgta gcggttaaca ggctggtcgc aaccagcagt tctgccagca
    14221 cgtttttaac gggctgcgga taatcgtggt tctcaaggat ctgttgcagg gtttccgaaa
    14281 cggttaccag ttcgccgcga acggcaaaat tttcaaacag atagcgatgt aattggtcat
    14341 gttgcggcat aatcatctct cttgcaggtg acagttattc actgtcgccg tgtttaaatc
    14401 gtaacaggtc gcggcgctct tttttgtccg ggcgtcggtc cgggtgcggc atggttaagg
    14461 cattaagttt acgtgccatc gccatttttt cgcgtttctc tacactttcc gcagtctctt
    14521 catacagcaa ggctgcctcg ctggcggggc gacgctgttc agtaatcgcc tttacaatca
    14581 ccgtgcgttc gtcatttccc tggcgcagag tgagcgtggc attcagctcg acgattttgc
    14641 tcggcttgct gcgctgcccg ttgtaatgca ccttaccgcc ttcaatcatt tcacgggcca
    14701 gcgcgcgggt tttataaaaa cgggcagccc atagccattt atccagtcga acctcaacag
    14761 caggtttctc tttcatggcg tctccttcac attagcgagg ggatcaggcg gcggtagtca
    14821 ttcagtgacg gatggcgttg atactgtttc tcggcaatcc cggaatcagg attagtcacg
    14881 ccgaggcagt aacgaatacc aaattgcgcg gcagcatcga gaatcgcttc gctgtcatca
    14941 ataaacagcg ttctttcagc tttcagaccc gtagcttcgg ccaccgcatg ccataaccgc
    15001 tgatcctctt tcggataacc aaatgtgtgg gtggaaagta ataaatcaag gtgtgcgtcc
    15061 agacccgtat gctcaagttt taccgccagg ttgtgcggat gagcattggt gagcaaaatt
    15121 cgctgcttac cgctggcttt cagtgcctca agaaacggaa tggtatcttc acgcagtacg
    15181 gcgcgcggtc ccatctcggt ggtcatcgca cagatatcca gacccagttg ctcactccag
    15241 taatcaagac agtaccagtt tagcgtatgc tgtacgtcgt gatattgctg gcgcatatat
    15301 tccatcgctt cctgtggcgt aaccccgttt ttcgcgcccc atgtttcagg caccagcttt
    15361 tgccagaaat agttatcgaa ggcgaggtcg agcaacgtgc cgtccatatc cagcagaacg
    15421 gtatctacgt cctgccaggc aatgttgata tgcatgaggg aaatctccag agtgaagcaa
    15481 tttgcgcgac agggtagcat aacctgccgc gcaaacgtgt tattcgataa ggctttctga
    15541 aggggtgatc agttgcgggt tcaggcagct ttcataatag gcctgaatct ccatcatgcg
    15601 agtgcgatga cgctggtaac gacgccaggc ctgtacgcca ttgtaaatgg cgctacccag
    15661 catcgtcagt agcagcaatg tggtgctgag atagcgccac aggccggaac gatccgggat
    15721 cggatgaagg ccaatatgct gcgtaccatt ggcgtcagtg aagatttttg tgacgatacc
    15781 ttcggcgtta aacggcgtat gcatcagcat ttgtgccagt ttctggaaag cgttccactg
    15841 ttcttgcggc gggtagtcgt aaagtgatgc cgaaggccag ggctgatcaa caaaatcgct
    15901 gccttcgtcg ctgacaatca ggaatccgcc gggggccgga ctgtttagtg attgtgccgc
    15961 tcgcgccgtt tcatgcgtga taaacggcgc ggtggaggtt gccaccaggt tatccagcga
    16021 ttcagcactc accgggcgta ataacacatt cacgccatcg agcttcccgg cgttggcgcg
    16081 ttttaccagc gcgtcccagt ctttactgtt gccgagattg accagcgcat ttttcagtcg
    16141 cacacagtca tctttggcag aacataaatc cgctgtcttc agtacaatgt cgccaaaatc
    16201 atcaagcaat accatgccgg atttttgaat cgctgaacgt aatgaggcac tgacgcgaga
    16261 ttcatcttcc ggtttagggt gcagctggcg attaactgct tcagtcaatg ccgtcgcttt
    16321 gttgaccagt tcagattctg gtaatggtaa tgagcgggcg tcattccaga tgatctgcga
    16381 gcagtcaaac ggtaaaaaag gtgaattggt tttcgcgctc caggttccgg aagtgcgaat
    16441 attacacatt cccgtaccgc taatacgcaa tgtatcgcct acccgcacgc cagcgtcagc
    16501 cagttgtttt acgctggtgg cttcaatggt ctgcgcgcct ttcatccatg agagagtgaa
    16561 tttcagcggc atatccagcg ggatccagaa taacagcata aacagcacta gcagcgagcc
    16621 cgcagcgata atcgtactgc gcagccagtg ctgtaacgga aagtttttta cttcatcatg
    16681 cagcgaaaga tatcgcccct gacgcactac gtggcggtcg agatagatat caatatcggt
    16741 ttgttgaccg agatcctgag caatgtatgg ctgccagtgt gcgggataga ccaggtcgat
    16801 aataccgagt gaaatattgt tgatctgttc ctgatcgttt tcgccaaaca atccccaacg
    16861 acggggtgta ccgcgcagac aatgaatttc tcgcagggag gattttgccg ggggcgcgaa
    16921 tagcccccac agacctgcgc ccagcagcag taatgcgccg cctgccagcc acggaacaaa
    16981 tacatccggg gtaatcaggc agaaaaagaa catcaggaag gaggcgacga tcagcaacgc
    17041 ttcacgcagc ccgcgcggac gactcagcgc atattcttca tgcgtttctt tgcgaatatt
    17101 gagtagctcg atttgctcac tctcttcccc gcgaatcgac gcctgcgtgg acggaacagg
    17161 ttgcaaggca tagccgcgcg tttcctgcat gtactcctgc agcgtatgac cgttgagtga
    17221 aataaccaac ggcagcgaat cggtatgaat cagttcaacg gtattctcat cgttgatgta
    17281 ctgttcccag aaggggggca ggtggacttc taccgaatcg aggtagtaac gccatttatt
    17341 cggatcgtcg gtagagatgc cgtaacgtgt gatagcgtgt gtcagcatca tgacgttgtt
    17401 gctttcggca ttcagcgcca gagagatcgg tgccgcgcta gcttccgttg ggccaggcac
    17461 ctgtaatacc tgcgtcaggc tctcaagata attttcaacg gcgctacgtt cttcaggtgt
    17521 gagtttacgc gtttgcgcat ccgcgaaggc gttggtccag ggcagctgac gccgtctgga
    17581 tcgcactttt atcagccatc ccgcaagtag tgagcaggcc agcaaagcag ctaaaaaaat
    17641 cacaatggtg ctcatgcttt ccccatctta cttagtctgt caggcgtggt cagtcgcacc
    17701 ggacacaata catgattctg cggttggcta tcggcaagcg tggagcttaa cttgagcatc
    17761 attaagcaca tcacagtaac cgctgatcat agcaaaccct ctgaaggcgg aatatcagca
    17821 aattcctgga gttatttcaa cctattgact tttaatttga tttgaggacg attatttatt
    17881 aagttacata aatgtaaata acatgcaatt cacattgccg aaagcgtgtc cgatatcgca
    17941 caataaccgc tgatttcaca gcatagatcc cacgatgaac aaatcattac aaaaacccac
    18001 cattctgaat gttgaaactg tagcccgttc ccgactgttt accgtcgaga gcgtggatct
    18061 ggagttcagc aatggcgtgc ggcgtgttta tgaacgaatg cgtccaacca accgggaagc
    18121 agtgatgatt gtgccgattg tggacgatca cctgatcctg atccgcgaat acgcagtggg
    18181 aactgaatcc tacgaattag gtttttcgaa aggattaatt gatccgggtg aaagcgtcta
    18241 cgaagccgct aaccgcgagc taaaagaaga ggttggattt ggtgcgaacg atctgacttt
    18301 tttgaagaag ctcagcatgg caccgtctta cttttccagc aaaatgaata tcgtggtcgc
    18361 gcaagatctc tacccggaat cgctggaagg cgatgagcca gagccgctac cacaggtgcg
    18421 ctggccgctg gcgcatatga tggatttgct ggaagaccct gacttcaacg aagcgcgtaa
    18481 tgtcagcgcg ctgttcctcg tgcgcgaatg gttgaaaggg caggggcgag tgtaaatgcg
    18541 tgaagtttgc ctgatgcaca acgcttatca ggcttatggg gttcctgcaa tatattgatt
    18601 tttcacgatt ttgttagccg gataacgcgt tcacccgcat ccggcataaa caaatgcact
    18661 ttgtcagcca actgaaaagg cgccgaagcg cctttttaat cagaacaatt cctgtgcctc
    18721 accattatcg ataatggtcg ttcccacctc gtgcaccgcc tgttgtgtcg gctgcgtacc
    18781 ttcgatgaaa tactcttcgc ggctgttgcc accattagct aactgcccgg tgctgcgatc
    18841 gatattcacc gtcacaatac ccggaggcgg cgtcagcggc tgctccggca caccttcaag
    18901 aacggctttc atataagcgt cccatgcagg ctgggcactc ttggcaccgc cttcgtaacc
    18961 tgagatctga tctttaatcg ctccggaagc cgttgtatga ccgagattac gacggtgatc
    19021 atcaaagcca atccagaccg aggtcacaac gcccggaccg taacccgaga accacgcatc
    19081 tttcgaactg ttagtggtac cggttttccc gccgatatcg cgacgctgca aatcacgacc
    19141 tgcacgccag ccggtaccct gccagcctgg ttcaccaaag atattggtgt tcaaagcact
    19201 cttaatcagg aatgccagcg gagtgttgat gacgtgcggt gcgtactcct gcgcgccagt
    19261 cttcgccact aacgcctgat ttgcctgctc cagctgcggc attggtacag aaacattctg
    19321 ctgctcgcgg gagatagcga catcttcaac atcgttattt tccagcacgt tcgatttctg
    19381 cgtatcaccg taaatcactg gaatatcgca ttccgggcag gctactttcg gtttcgcttc
    19441 gaaaatcacg ccgccctgat cgttttcaat tttgctgata aaccacgggt ccactaggaa
    19501 gccgccgttc gccatgaccg cgtagccgcg cgccacctgc attggggtga aggacgctga
    19561 acccagcgcc agcgattcgg tgtggacaat gttttgcgcc gggaagccga agcgttgcag
    19621 atattctgca gcgtagtcga cgcccatcgc ccgcattgca cgtaccatca ccacgttttt
    19681 cgactgaccc agcccctgac gtaagcgaat tggaccagca tactgcggtg gtgagttctt
    19741 cggctgccag tcagaaccgg cacctgcatc ccagcgagaa attggcacat cgttcaacat
    19801 acttgccagc gtcagacctt tatccatcgc cgcggtgtag aggaacggtt tgatgttgga
    19861 acccacctga cgcagtgcct gggtggcgcg gttaaacttg ctctgattga aatcaaagcc
    19921 accgaccagc gccataacgg caccattttg cggattgatc gacaccagcg ccgagttcac
    19981 ttccggcact tgtgccagcc accatgcatc gccaacctga cgaacccaga tttgctgacc
    20041 cgtttgcaga acatcggtca ctttacgcgg cgtcggtccc tgctgagtat ccgaacggta
    20101 aggacgcgcc cagcgaacgc cttccatact caatgcgacg gtcgacccgt ccgccagcat
    20161 cgccgtcgct ccctgaggat tggcgctggt gactgcggca ggcagcagcg gaccataggt
    20221 cggcagcgcc ttcagcgtat cggtaatctt gttgttatcc cacgccgact cgcccacttt
    20281 ccacagcaca tttgccgggc cgcgatagcc gtggcgcatg tcgtagtcca gcacgttatt
    20341 acgtaccgcc tgctgcgcgg cctgctgcac tttgcgggtg atggtggtgt aaatgcgata
    20401 accgtcttca taggcacttt cgccataacg gttatacatc tcctggcgca ccatttcgct
    20461 caggtacggc gcagagaaag caatctccgg cgcgtgataa ttagcgttaa tcgcctcagt
    20521 gcgtgtctga tcgaactgtt gctgggtgat atacccttca tccagcatcc gcgacagtac
    20581 gacgttacgc cgcgcgacgg cacgatccat tgagtagagc ggattaaagg tggaaggcgc
    20641 tttcggcaac ccggctatca ccgccatttc gttcagcgtc agttggtcga ccgttttacc
    20701 gaaatagact tgtgccgcag caccgacacc ataggcgcgg taaccaaggt aaatcttgtt
    20761 cagataaagc tcgaggattt cgtctttcgt caacaactgt tcaatgcgaa tcgcgaggaa
    20821 gacttcctta atcttacgca tcatcgtgcg ttctggactg aggaagaaat ttctcgccag
    20881 ctgctgggta atggtactcg ccccttgtga cgcgtggccg gagaacagcg ccacacttgc
    20941 cgcacggaag atccccaccg ggtcaacgcc gtgatgctcg tagaagcggc tgtcttccgt
    21001 cgcgataaag gctttcacca tctccggtgg gatttgatcc aacgtaaccg gaatacgacg
    21061 tttctcaccg tattgagcaa tcagctcgcc atcggcgctg taaatctgca tcggaatttg
    21121 caggcgaaca tcttttaatg tcaccacatc cggcagttgt ggctcgatgt agcggtatag
    21181 gccataaatc gagcctgctc ccagcagaat gcaacagact gcaaggatca aaaaatactt
    21241 tacgaacttc actggaaatt tcccatttag tttcatttgg gcagtttata aacaaacgcg
    21301 cggtagtata aaggcaagcc agacgcattg atatacccgt cagagtgacg ggtgataagg
    21361 agatcatcac aatgacattt aagatctggc aaattggttt gcatttacaa cagcaagaag
    21421 cggcagcggt tgcgatcgtg cggggcgcaa aagaatgctt tttgcaacgc tggtggcggt
    21481 tgccgctggc gcacgacatt atcaaagatg ggcggattgt tgatgcgcag cagctggcta
    21541 aaacgttgtt accgtggagt cgcgaactgc cgcagcgtca tcacattatg ttggcgtttc
    21601 ccgccagtcg cacattacag cggtcatttc cgcgcccgtc gatgtccctt ggtgagcggg
    21661 agcaaacggc ctggctgtca gggacgatgg cccgcgagct ggatatggat ccggactccc
    21721 tgcgcttcga ttacagcgaa gactcactca gccccgctta taacgtgact gccgcgcaaa
    21781 gcaaagagct ggcaacgctg cttacgctgg cagaaaggtt gcgtgttcat gttagtgcga
    21841 tcaccccgga tgccagtgca ttacagcgat tcctgccttt tttaccttct catcagcaat
    21901 gtctggcctg gcgtgataac gaacagtggc tgtgggcgac gcgctatagc tgggggcgca
    21961 aactggcggt ggggatgact agcgcgaagg agctggcggc agcgttatcc gttgatcccg
    22021 acagcgtcgc gatatgtggc gaaggcggat ttgacccctg ggaggccgtt tctgttcgtc
    22081 agccgccgct accgccgcct ggtggagact ttgccatcgc gctggggctg gcgcttggga
    22141 aggcgtactg atgaatccgc caattaattt tttgccctgg cgacagcaac gccggaccgc
    22201 ttttctgcgt ttctggttgc tgatgttcgt tgcgcctctg ctgctggccg tcgggataac
    22261 gctaatactg cgtctgacag gcagcgccga agcgcgcata gacgcagttt tgcttcaggc
    22321 ggaacaacaa ctcgcccgca gcttacagat aacgaagcca cgtttgctgg agcaacagca
    22381 gttacgcgaa cagcgttcgc aacggcagcg ccagcgacaa tttacccgcg actggcaatc
    22441 tgcgctggaa gcactggcgg cgcttttacc cgagcacgcc tggctgacaa cgataagctg
    22501 gcagcaggga acgctggaga tcaaggggct gacaacaagc attaccgcgt taaacgcact
    22561 agaaacgtca ctccgccagg atgcgtcttt tcatctcaat cagcggggag ccacgcagca
    22621 ggatgcgcag ggacgctggc aatttgagta tcagttaaca aggaaggtta gcgatgaaca
    22681 tgttctttga ctggtggttc gccacatcac cccgcctccg ccagttttgc tgggcattct
    22741 ggttgctgat gttagttacg ctcatttttc tatcatcgac acaccatgaa gagcgcgacg
    22801 cattaattcg actacgggca agtcatcacc agcagtgggc cgcactgtat cgcctggtag
    22861 acaccactcc cttcagcgag gaaaaaacgc tgcccttttc gccactggat tttcagttat
    22921 ccggcgcgca actggtttcc tggcatccat ccgcgcaggg aggtgagttg gcgttgaaaa
    22981 cgctgtggga agcagtgccg tcggcattta cacggctggc agagcgcaac gtcagcgtga
    23041 gccgtttttc gttaagcgtg gaaggtgatg atcttttgtt cacgctacaa ctggagatgc
    23101 cgcatgaggg ttaaacgctg gttgttggca ggtattgcat tgtgcctttt aaccggtatg
    23161 cgtgaccctt ttaaatcgcc ggaagatcta tatgccggat tagcgaactt agccagtggc
    23221 gctatcaggg gatggtaggg cgaggcgagc gcatcatcgg tgtaataaaa gacgggcaaa
    23281 agaaatggcg acgggtgcag caaaacgatg tgctggaaaa cggctggaca attttacagc
    23341 tgacgccaga cgtactaacg ctgggtaccg ggacaaactg cgaaccgcca caatggttgt
    23401 ggcaacggca aggagataca aatgaagcaa tggatagccg cactactgtt gatgctgata
    23461 cccggcgtac aggcggcaaa gccgcaaaaa gtgacgctga tggtggatga cgttccggta
    23521 gctcaggtgt tgcaggcgct ggctgaacag gagaagttga acctggtcgt gtcgccagac
    23581 gtcagcggta cggtgtcgtt acatctaaca gatgttccct ggaagcaggc actacaaact
    23641 gtagtgaaaa gcgccggact gataacgcgg caggaaggca acattctctc agtgcattcc
    23701 attgcctggc agaatgacaa tatcgcccgc caggaggcgg agcaggcgcg ggcgcaggca
    23761 aatctgccgc tggaaaatcg cagtataacc ctgcaatacg ccgacgcggg agaactggcg
    23821 aaagcggggg agaagctact gagtgccaaa gggagtatga ccgtcgataa acgcaccaat
    23881 cgccttttgc tacgagataa caaaacggcg ttaagcgcgc ttgaacagtg ggtagcgcaa
    23941 atggatctgc cggtcgggca ggttgagctg tcggcgcata ttgtcaccat taatgaaaaa
    24001 agtttgcgtg agttaggcgt gaaatggacg ctggccgatg cgcaacacgc tggtggcgtt
    24061 gggcaagtca ccacgcttgg tagcgacctc tccgtagcga cggcgacaac gcatgtcggt
    24121 tttaacattg ggcgcatcaa cggacgcttg ctggatcttg agctttccgc gctcgaacaa
    24181 aaacagcagc tggatattat cgccagtccg cgtctgctgg cctcacatct tcagcctgcc
    24241 agcattaaac aggagagcga aattccatat caggtttcca gcggggaaag tggcgcgacg
    24301 tcggtggaat ttaaagaggc cgtcctgggg atggaggtca tgcccacggt gttacaaaaa
    24361 ggtcgcatcc ggctgaaatt acacatcagc cagaacgttc cggggcaggt gctacagcag
    24421 gccgatggcg aagtgctggc gattgataag caggagatcg aaacgcaggt cgaggtaaaa
    24481 agcggagaaa cgttggcgct gggcggcatt tttacccgta aaaataaatc gggtcaggat
    24541 agcgtaccgt tgcttggcga cattccctgg ttcgggcaat tatttcgtca tgacggaaaa
    24601 gaagatgaac gacgcgagtt agtggtgttt atcacgccac gactggtttc cagtgagtaa
    24661 acagccgtaa aagcggtaat gtttttatgc tgaacgtgtt tcatctattt gacgcgcgca
    24721 ggtatttagc atacaaggag taccgatttg agagttggtg ctcttcgctg cctgcgttcc
    24781 atgatgatga tttatcattc aggcggcatt ttgctgtctt ttttacgcta atcttacccg
    24841 gtgatttatc gccagagcgg tggtagcaag gcagcgcgct tgcagcgacc agatatgcag
    24901 agggatgggt gatttattca gttgccaaac ccgctggagt attgagataa ttttcagtct
    24961 gactctcgca atatcttatg aggtttcagt tcatgtcctg cggcgctctc tgagcgaagc
    25021 gggtttatca ttaacgaata gtcttagtag taccgaaaaa atggcagaga aacgcaatat
    25081 ctttctggtt gggcctatgg gtgccggaaa aagcactatt gggcgccagt tagctcaaca
    25141 actcaatatg gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt
    25201 gggctgggtt ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat
    25261 caatgagttg accgagaaac agggtattgt gctggctact ggcggcggct ctgtgaaatc
    25321 ccgtgaaacg cgtaaccgtc tttccgctcg tggcgttgtc gtttatcttg aaacgaccat
    25381 cgaaaagcaa cttgcacgca cgcagcgtga taaaaaacgc ccgttgctgc acgttgaaac
    25441 accgccgcgt gaagttctgg aagcgttggc caatgaacgc aatccgctgt atgaagagat
    25501 tgccgacgtg accattcgta ctgatgatca aagcgctaaa gtggttgcaa accagattat
    25561 tcacatgctg gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat
    25621 taaggtggat gtcgcgttat ggagaggatt gtcgttactc tcggggaacg tagttaccca
    25681 attaccatcg catctggttt gtttaatgaa ccagcttcat tcttaccgct gaaatcgggc
    25741 gagcaggtca tgttggtcac cagcgaaacc ctggctcctc tgtatctcga taaggtccgc
    25801 ggcgtacttg aacaggcggg tgttaacgtc gatagcgtta tcctccctga cggcgagcag
    25861 tataaaagcc tagctgtact cgataccgtc tttacggcgt tgttacaaaa gccgcatggt
    25921 cgcgatacta cgctggtggc gcttggcggc ggcgtagtgg gcgatctgac cggcttcgcg
    25981 gcggcgagtt atcagcgcgg tgttcgtttc attcaagtcc cgacgacgtt actgtcgcag
    26041 gtcgattcct ccgttggcgg caaaactgcg gtcaaccatc ccctcggtaa aaacatgatt
    26101 ggcgcgttct accagcctgc ttcagtggtg gtggatctcg actgtctgaa aacgcttccc
    26161 ccgcgtgagt tagcgtcggg gctggcagaa gtcatcaaat acggcattat tcttgacggt
    26221 gcgtttttta actggctgga agagaatctg gatgcgttgt tgcgtctgga cggtccggca
    26281 atggcgtact gtattcgccg ttgttgtgaa ctgaaggcag aagttgtcgc cgccgacgag
    26341 cgcgaaaccg ggttacgtgc tttactgaat ctgggacaca cctttggtca tgccattgaa
    26401 gctgaaatgg ggtatggcaa ttggttacat ggtgaagcgg tcgctgcggg tatggtgatg
    26461 gcggcgcgga cgtcggaacg tctcgggcag tttagttctg ccgaaacgca gcgtattata
    26521 accctgctca cgcgggctgg gttaccggtc aatgggccgc gcgaaatgtc cgcgcaggcg
    26581 tatttaccgc atatgctgcg tgacaagaaa gtccttgcgg gagagatgcg cttaattctt
    26641 ccgttggcaa ttggtaagag tgaagttcgc agcggcgttt cgcacgagct tgttcttaac
    26701 gccattgccg attgtcaatc agcgtaacaa caagaaaggt caggccgctt atcaagcggt
    26761 ctattagctt caggttaatt gcaacgtggt aagcattaac cttttagtgg ggtgttaaat
    26821 ggatgaattc aaaccagaag acgagctgaa acccgatccc agcgatcgtc gtactggtcg
    26881 ttctcgtcaa tcttctgaac gttctgagcg tactgaacgt ggcgaaccgc agatcaattt
    26941 tgatgatatt gaacttgatg acactgacga tcgccgtccg actcgtgcgc aaaaagagcg
    27001 caatgaggaa ccggaaatcg aagaagaaat tgacgaatcc gaagatgaaa ccgtggatga
    27061 agagcgcgta gagcgtcgtc cgcgtaagcg caaaaaagca gccagtaaac ccgcttctcg
    27121 tcagtatatg atgatgggcg tcggcattct ggttctactg ctgttgatca tcggtatcgg
    27181 ttctgcgcta aaagccccct cgaccacttc cagcgatcaa accgcgtctg gcgagaagag
    27241 tattgatctt gctggcaatg cgaccgatca ggcgaatggc gtgcagccag cgccgggaac
    27301 cacgtctgcg gaaaatactc agcaggatgt ttctctgcca ccgatctctt ctacgccgac
    27361 tcaagggcaa accccggtgg caacggatgg tcaacaacgt gttgaagtgc agggtgacct
    27421 gaacaatgcg ctgacccagc cacaaaatca gcaacagctg aacaatgtgg cggtcaattc
    27481 cacgttgccg actgaacccg caacggttgc gcctgttcgc aatggcaatg catcgcgtga
    27541 cacggcgaaa acgcaaaccg ctgaacgtcc gtccactacg cgcccagttc gtcagcaggc
    27601 ggtgattgaa ccgaaaaaac cgcaagcaac cgtgaaaacg gagccgaagc cggtagcaca
    27661 gacgccgaag cgtactgaac ctgaaccagc tgcccctgtg gcgagcacga aggcaccggc
    27721 tgcgacttct acgccagcac caaaagagac ggcgactacg gctccagtac agacggcatc
    27781 cccggcgcaa accacggcaa caccagccgc tggagggaag accgcaggta atgttggttc
    27841 gttgaaatcg gcaccgtcca gccattacac tctgcagctg agcagttcct ctaactacga
    27901 caacctgaac ggttgggcga agaaagagaa tctgaaaaac tacgttgtct atgaaacgac
    27961 gcgtaatggt cagccgtggt atgtcctggt ttctggcgtg tacgcttcga aagaagaggc
    28021 gaaaaaagcg gtatctacat tgccagcaga tgttcaggcc aaaaacccgt gggcgaaacc
    28081 gctgcgtcag gtacaggccg atctgaagta atcaaggtta tctcccgcaa tggtttatcg
    28141 ttgcgggagc tgcctgaagc gctggatgct gtcggagctt tctccacagc cggagaaggt
    28201 gtaattagtt aatcggcatg aagaaaaatc gcgctttttt gaagtgggca gggggcaagt
    28261 atcccctgct tgatgatatt aaacggcatt tgcccaaggg cgaatgtctg gttgagcctt
    28321 ttgtaggtgc cgggtcggtg tttctcaaca ccgacttttc tcgttatatc cttgccgata
    28381 tcaatagcga cctgatcagt ctctataaca ttgtgaagat gcgtactgat gagtacgtac
    28441 aggccgcacg cgagctgttt gttcccgaaa caaattgcgc cgaggtttac tatcagttcc
    28501 gcgaagagtt caacaaaagc caggatccgt tccgtcgggc ggtactgttt ttatatttga
    28561 accgctacgg ttacaacggc ctgtgtcgtt acaatctgcg cggtgagttt aacgtgccgt
    28621 ttggccgcta caaaaaaccc tatttcccgg aagcagagtt gtatcacttc gctgaaaaag
    28681 cgcagaatgc ctttttctat tgtgagtctt acgccgatag catggagcgc gcagatgatg
    28741 catccgtcgt ctattgcgat ccgccttatg caccgctgtc tgcgaccgcc aactttacgg
    28801 cgtatcacac aaacagtttt acgcttgaac aacaagcgca tctggcggag atcgccgaag
    28861 gtctggttga gcgccatatt ccagtgctga tctccaatca cgatacgatg ttaacgcgtg
    28921 agtggtatca gcgcgcaaaa ttgcatgtcg tcaaagttcg acgcagtata agcagcaacg
    28981 gcggcacacg taaaaaggtg gacgaactgc tggctttgta caaaccagga gtcgtttcac
    29041 ccgcgaaaaa ataattctca aggagaagcg gatgaaacag tatttgattg ccccctcaat
    29101 tctgtcggct gattttgccc gcctgggtga agataccgca aaagccctgg cagctggcgc
    29161 tgatgtcgtg cattttgacg tcatggataa ccactatgtt cccaatctga cgattgggcc
    29221 aatggtgctg aaatccttgc gtaactacgg cattaccgcc cctatcgacg tacatctgat
    29281 ggtgaaaccc gtcgatcgca ttgtgcctga tttcgctgcc gctggtgcca gcatcattac
    29341 ctttcatcca gaagcctccg agcatgttga ccgcacgctg caactgatta aagaaaatgg
    29401 ctgtaaagcg ggtctggtat ttaacccggc gacacctctg agctatctgg attacgtgat
    29461 ggataagctg gatgtgatcc tgctgatgtc cgtcaaccct ggtttcggcg gtcagtcttt
    29521 cattcctcaa acactggata aactgcgcga agtacgtcgc cgtatcgacg agtctggctt
    29581 tgacattcgt ctggaagtgg acggtggcgt gaaggtgaac aacattggcg aaatcgctgc
    29641 ggcgggcgcg gatatgttcg tcgccggttc ggcaatcttc gaccagccag actataaaaa
    29701 agtcattgat gaaatgcgca gtgaactggc aaaggtaagt catgaataag tttgaagata
    29761 ttcgcggcgt cgcttttgat cttgatggta cgctggtcga cagtgctcct ggtcttgctg
    29821 ctgcggtaga tatggcgctg tatgcgctgg agttgcccgt cgcaggtgaa gaacgcgtta
    29881 ttacctggat tggtaacggc gcagatgttc tgatggagcg cgcattgacc tgggcgcgtc
    29941 aggaacgtgc gactctgcgt aaaacaatgg ggaaaccgcc cgttgatgac gacattccgg
    30001 cagaagaaca ggtacgtatt ctgcgtaaac tgttcgatcg ctactatggc gaggttgccg
    30061 aagaggggac gtttttgttc ccgcacgttg ccgatacgtt gggcgcgttg caggctaaag
    30121 gcctgccgct aggcttggtc accaacaaac cgacgccgtt cgtcgcgccg ctgctcgaag
    30181 ccttagatat tgccaaatac tttagcgtgg ttatcggcgg cgatgatgtg caaaacaaaa
    30241 aaccgcatcc ggacccgctg ttactggtgg ctgagcggat gggaattgcc ccacaacaga
    30301 tgctgtttgt cggcgactca cggaatgata ttcaggcggc aaaagcggca ggttgcccat
    30361 cagttggctt aacctacgga tacaactacg gcgaggctat cgatctcagc cagcctgatg
    30421 taatttatca gtctataaat gaccttctgc ccgcattagg gcttccgcat agcgaaaatc
    30481 aggaatcgaa aaatgactaa gcccatcgtt tttagtggcg cacagccctc aggtgaattg
    30541 accattggta actacatggg tgcgctgcgt cagtgggtaa acatgcagga tgactaccat
    30601 tgcatttact gtatcgttga ccaacacgcg atcaccgtgc gccaggatgc acagaagctg
    30661 cgtaaagcga cgttggatac gctggccttg tatctggctt gtggtatcga tcctgagaaa
    30721 agcaccattt ttgttcagtc ccacgtacca gaacatgcgc agttaggctg ggcactgaac
    30781 tgctatacct acttcggcga actgagtcgc atgacgcagt ttaaagataa atctgcgcgt
    30841 tatgccgaga acatcaacgc tggtctgttt ggctatccgg tgctgatggc tgcggacatc
    30901 ctgctgtatc aaactaatct ggtaccggtg ggtgaagacc agaaacagca cctggaactg
    30961 agtcgcgata tcgcccagcg tttcaacgcg ctgtatggcg agatctttaa ggtgccggag
    31021 ccgtttattc cgaaatctgg cgcgcgcgta atgtcgctgc tggagccgac caagaagatg
    31081 tccaagtctg acgataaccg caataacgtt atcggcctgc tggaagatcc gaaatcggta
    31141 gtgaagaaaa tcaaacgcgc ggtcactgac tccgacgagc cgccggtagt tcgctacgat
    31201 gtgcagaaca aagcgggcgt ttccaacctg ctggatatcc tttctgcggt aacgggccag
    31261 agcatcccag aactggaaaa acagttcgaa ggcaagatgt atggtcatct gaaaggtgaa
    31321 gtggctgatg ccgtttccgg tatgctgact gaattgcagg aacgctatca ccgtttccgc
    31381 aacgatgaag ccttcctgca acaggtgatg aaagacggcg cagaaaaagc cagtgtacac
    31441 gcttcccgta cgctgaaagc ggtgtacgaa gcgattggtt ttgtggcgaa gccgtaatat
    31501 caaaacgctc ggacggtccc ctcgcccctt tggggagagg gttagggtga ggggagcagg
    31561 caagcgccag tgggaattac cctttgctcg cattgtcgaa taaatcactt cgtcatcact
    31621 ctgaaaccgg ggaaacccgg tttttttatc ctcttttcgc aacaacccga aaaaatgtga
    31681 agcgcctcgc cgtttccaca tctcgtcgtc gccacttatc ttcattttaa tgaagatata
    31741 aatattcatt ttattgaaaa tatattatgc gtcgaacgtt tatcaaaaaa gaaggcgtcg
    31801 tcatcacgac actggcccgt tatttgttgg gtgaaaaatg cggtaatcga ctgaaaacca
    31861 tagatgagct ggcaactgaa tgccgttcat cggttggcct gacgcaggcc gcgttgaaaa
    31921 cgctggaatc aagcggagcg atacggattg aacgccgtgg gcgcaatggc agttatcttg
    31981 tcgagatgga taacaaagca ttgctgagtc atgtggatat caacaacgtg gtatgtgcga
    32041 tgcctttgcc ctatacccgc ttgtacgaag gtttagcgag cggattgaaa gcccagtttg
    32101 atggcattcc tttttactat gcgcatatgc gcggcgcgga tattcgcgtg gagtgtttac
    32161 ttaacggtgt ctatgacatg gcagtagttt cccgcctggc ggcggaaagt tatctcagcc
    32221 aaaacaattt gtgcatcgcc ctcgagcttg gcccgcatac gtatgttggt gaacatcaac
    32281 tgatatgccg caaaggtgag tctggaaatg ttaagcgagt aggtctcgat agtcgttcgg
    32341 cggatcagaa aatcatgacc gatgtctttt ttggcgatag tgatgtggaa cgagtcgatc
    32401 tctcttatca cgagagttta caacgcattg ttaaaggcga tgtcgatgcg gtgatctgga
    32461 acgtggtggc ggaaaacgaa ctgaccatgc tgggattaga ggcgacgccg ctaacagacg
    32521 atccgcgatt tttacaggcc accgaagccg tggtcctgac gcgagtcgat gattacccaa
    32581 tgcaacaatt gctgcgtgct gttgtagata aacacgccct gcttgcccat caacaacggg
    32641 tggtgagtgg ggaacaggaa cccagttatt aaacgaaagg catctgatat ggaaaccaga
    32701 ctcaacctgc tttgcgaggc aggcgttatt gataaggacg tctgcaaagg catgatgcag
    32761 gtcgtcaacg tactggaaaa agagtgccat ctgccggtgc gcagtgagca aggaacgatg
    32821 gcgatgacac atatggcgag tgcactgatg cgcagtcgcc gtggtgaaga aatagagccg
    32881 ctggataacg agttgctggc ggaactggcg caatccagcc actggcaagc cgttgtgcaa
    32941 ttgcatcagg agttgttgaa ggaattcgct ctggaagtta acccgtgtga agaaggctat
    33001 tttctggcca atctttatgg attatggatg gctgctaacg aagaggtttg aagctcccga
    33061 caaatccacg tcgcacaacc taaatattca aaaaaatgaa tatttaattc aaggaacaga
    33121 aacgaggcaa caagatgttt gtagaagcat tgaaacgcca gaacccggcg ctgatttccg
    33181 ccgcactaag tttgtggcag cagggcaaaa tcgccccgga cagctgggtg atcgacgtgg
    33241 atcagatact ggaaaacggt aagcggctca ttgagacggc gcggctttac ggcattgaac
    33301 tgtatctgat gaccaagcaa tttggtcgta atccgtggct ggcggaaaaa ttgctggcat
    33361 taggctacag cggcattgtg gcggtggatt acaaagaggc gcgagtcatg cgccgcgctg
    33421 gtttgccagt ggcgcatcag gggcatctgg tacaaatccc ttgtcatcag gttgctgacg
    33481 ccgttgaaca gggcaccgac atcatcaccg tgtttactct cgacaaagcg cgggaagttt
    33541 ctgcggcagc ggttaaggcc ggacgggtcc agcctgtgct gctcaaagtt tatagcgacg
    33601 atgattttct ttatccgggg caggagagcg gttttgtaca gcattcgctg cacgaggtga
    33661 ttgctgaaat taagaaactg ccggggctgc atttagccgg actaacccat ttcccttgcc
    33721 tgctttggga tgaggctgct gggaaagttt tgccaacacc gaatcttcac acgctggtac
    33781 aagcacggga tcaactggcg aaatctggta ttgcgattga gcaactgaac gtaccttcag
    33841 cgaccagctg cacttcgctg ccattgctgg cggaatacgg tgcgactcat gccgaacccg
    33901 gtcatgcgct gaccggtact attccggcaa accagcaggg cgatcagcct gaacgtatcg
    33961 cgatgctctg gttaagtgaa atctcccatc atttccgtgg cgacagctac tgctacggcg
    34021 gcggttacta ccgtcgtggt catgcgcaac atgcgctggt gtttacgcca gaaaatcaaa
    34081 agattactga aaccaatctc aaaactgtgg atgacagcag tatcgactac accctgccgc
    34141 tggcaggcga gtatccggtg agcagtgcag tggtgctctg ttttcgcacg cagatttttg
    34201 tcacccgtag cgatgtggtg ctggtgtccg gtattcagcg cggcgaacca gaaatagttg
    34261 gtcgttatga cagcctcggc aaccctctgg aggcataaat ggcgcgattt gtggtgttag
    34321 tgattgatag ctttggcgta ggggcaatga aagatgtcac gctggtgcgc ccgcaagatg
    34381 ccggggcgaa tacctgtggt cacatcctga gccagttgcc gcatttgcag ctaccaacgc
    34441 tggagaagct ggggctaatc aacacattgg gttatgcgcc aggcgatatg cagccgtcag
    34501 attccgcaac ctggggcgtg gcagagctgc aacatgaagg tggcgatacc tttatggggc
    34561 atcaggaaat tttaggcacg cgcccgttac cgccgctgcg gatgcctttt cgcgatgtga
    34621 ttgaccgtgt tgagcaggca ttagtttccg ctggctggca ggtggagcgc cgtggcgatg
    34681 atctgcaatt tctgtcggtc aatcaggcgg ttgcgattgg cgataatctc gaggcggatt
    34741 taggccaagt ctataacatt accgccaatc tctctgtgat ctcttttgac gacgcaatca
    34801 aaattggtcg tatcgtgcgt gaccaggtac aggtcggtcg ggtcattaca tttggcggcc
    34861 tgttaaccaa cagtcagcgt attctcgatg ccgcagaaag caaagaaggg cgctttattg
    34921 gtattaatgc gccgcgttct ggcgcttatg acaacggctt ccaggtcgtg catatgggct
    34981 acggtgttga tgaaaaagtg caggtgccgc aaaaactgta tgaagcgggc gtgccaaccg
    35041 tgctggtggg taaggtggca gatatcgtca gcaatcctta tggtgtgagc tggcaaaacc
    35101 tggtggatag ccagcggatt atggatatca ccctcgacga atttaacacc catccgacgg
    35161 cgtttatttg catcaatatc caggaaaccg acctcgctgg tcatgcagaa gacgtcgcac
    35221 gttatgccga acgtttgcag gtcgttgacc gtaaccttgc ccggcttgtt gaggcgatgc
    35281 agccagatga ttgcctggtc gtgatggcgg atcacggtaa cgatccgacc attggtcaca
    35341 gccaccatac ccgtgaagtg gtgccagtgc tggtttatca gcaagggctg gtcgccacgc
    35401 agctcggtgt gcgcaccacg ctttctgatg tgggagctac cgtgtgtgaa tttttccgtg
    35461 cgccaccgcc acaaaatggt cgctcttttc tttcctccct ccggtttgca ggagacaccc
    35521 tatgagtttt gatccgacgg gttacaccct ggcccatgag catctgcata ttgatctctc
    35581 cggctttaaa aacaacgtgg actgccgcct tgatcagtat gcgttcattt gccaggagat
    35641 gaacgacctg atgacccggg gtgtacgtaa tgtgattgag atgaccaacc gttacatggg
    35701 gcgcaatgcg caatttatgc tcgatgtaat gcgcgaaacc gggatcaacg tggtggcctg
    35761 tactggttat taccaggatg cgtttttccc ggaacatgtg gtgacccgca gcgtgcagga
    35821 actggcgcag gagatggtcg atgaaattga acagggtatc gatggcacgg agctgaaagc
    35881 cgggatcatc gcggagatcg gctccagcga aggaaagatt acgccgctgg aagagaaggt
    35941 atttattgcc gctgcgctgg cgcataacca gaccggacgc ccgatctcca cgcatacgtc
    36001 gttcagcacc atggggctgg aacaactggc tctgttgcaa gcccacggag ttgatctttc
    36061 gcgcgtcacc gttggtcact gcgatctgaa agacaacctc gacaacattt tgaagatgat
    36121 cgatctcggc gcgtacgtgc agttcgacac cattggtaag aacagttact acccggacga
    36181 aaagcgcatt gcgatgctcc acgcgctacg tgaccgtggg ttgctgaacc gcgtcatgct
    36241 gtcgatggat attacgcgcc gctcccattt aaaagccaat ggtggttatg gctatgacta
    36301 tttattgacc acctttattc cgcaattgcg ccagtcagga ttcagtcagg ccgatgtgga
    36361 tgtgatgtta cgtgaaaatc cctctcaatt tttccaataa ggacagactc atgaaaaaga
    36421 ttggcgttgc aggcttacag cgtgagcaga ttaaaaaaac cattgaagcg acggctcctg
    36481 gctgttttga agttttcatt cacaacgaca tggaagcggc aatgaaggtg aaatccgggc
    36541 aactagatta ttacattggc gcgtgtaata ccggtgcggg cgcggcattg tcgattgcca
    36601 tcgcggtgat tggctataac aaaagttgca ccattgccaa accaggcatt aaagcgaaag
    36661 acgagcatat cgccaaaatg atcgctgaag ggaaagtggc gtttggcctt tccgttgagc
    36721 acgtcgaaca cgcgattccg atgttgatta accatctgaa ataaaaggca cgactatgga
    36781 tctgtatatt cagattatcg tggtggcgtg cctgacgggt atgacatcgc ttctggagca
    36841 tcgctcggcg gctgtttttc atgacggcat ccacccgatc ctgccgcaac tgattgaagg
    36901 ctatatgaac cgtcgcgagg cggggagtat cgcttttggt ctgagcattg gttttgtggc
    36961 ctcggtgggg atctctttta ccctgaaaac cgggctgctc aacgcatggt tactctttct
    37021 tcctaccgat atcctcggcg tactggcgat aaacagcctg atggcgtttg gtcttggcgc
    37081 tatctggggc gtgttgatcc ttacttgcct gttgccagtt aaccagctgc tgaccgcgct
    37141 gccggtggat gtattaggta gcctcgggga attaagctcg ccggtggttt ctgcttttgc
    37201 actgttcccg ctggtggcga ttttctacca gtttggctgg aagcaaagtc tggtcgccgc
    37261 cgtggttgtt ctgatgaccc gtgtggtagt cgtgcgctat ttcccacatc ttaaccctga
    37321 atccatcgaa atctttattg gcatggtgat gctgctgggg atcgcgataa ctcacgacct
    37381 gcgtcatcgt gatgaaaatg acatcgatgc cagcgggctt tcggtgtttg aagagcgcac
    37441 gtcgcggatt atcaaaaact taccttatat cgccatcgtg ggagcattga ttgccgccgt
    37501 tgccagtatg aagatttttg ctggtagcga agtttcgatc ttcacactgg agaaagcgta
    37561 ttccgcaggc gtgacgccgg aacaatcgca aacgctgatt aatcaggcgg ctctggcaga
    37621 atttatgcgc ggactggggt ttgtgccgtt gattgccacc accgccttag caacgggtgt
    37681 gtatgcagtt gcgggcttta cctttgttta tgcggtgggc tatctctcgc cgaatccgat
    37741 ggttgcagcg gtattaggcg cagtggttat ttcggcggaa gtcttgctgc ttcgttcgat
    37801 cggcaaatgg ctgggacgct acccgtcggt gcgtaatgcg tcggataaca tccgtaacgc
    37861 catgaatatg ctgatggaag tggcgctgct ggtcggttcg attttcgcag caattaagat
    37921 ggcgggttat accggattct ctatcgcggt tgccatttac ttcctcaacg aatccctggg
    37981 ccgtccggta cagaaaatgg cggcaccggt cgtggcagta atgatcaccg gtattctgct
    38041 gaatgttctt tactggcttg gcctgttcgt tccggcttaa ggaaggcacc tatgaagacg
    38101 tttcctttgc aaagcctgac tcttgctgaa gcgcagcaaa agcagtttgc gctggtggac
    38161 actatttgcc gccattttcc aggcagcgag tttcttgctg gcggtgattt agggttaact
    38221 ccaagtctga atcaaccgcg tattacccaa cgggtggagc aggtgctggc tgatgcattt
    38281 cacgcacagg ccgcggcgct ggtgcagggc gcggggactg gcgcgattcg cgccgggctg
    38341 gcggctttgc tcaaaccggg gcagcgtctt ctggtgcatg acgcgcctgt ttacccgacg
    38401 acacgggtta ttattgagca gatggggctg acgcttatta ctgctgattt caatgacctg
    38461 tcggcactga agcaggtcgt cgacgagcaa caacctgatg ccgcgctggt gcagcatacg
    38521 cgccagcagc cgcaggacag ctatgtgctg gcagatgtgc tggcaacgtt gcgcgcggca
    38581 ggtgttccag cgttaaccga tgataactat gcggtgatga aggtagcgcg aatcggctgt
    38641 gaatgcggcg cgaatgtctc gacattctcc tgcttcaagc tgtttgggcc ggagggcgtt
    38701 ggtgcagtgg ttggcgatgc cgatgttatt agtcggattc gcgccacgct ttactccggc
    38761 ggtagccaga tccagggctc acaggcgctg gaagtcttgc gtggtctggt gttcgcgcca
    38821 gtgatgcatg cggtgcaggc gggagtatcc gaacggttgc tggctttgct taacggcggc
    38881 gcggtggcgg aagtgaaaag cgccgtcatt gccaatgcgc agtcgaaggt gttgattgtc
    38941 gagtttcatc agctgattgc cgccagggtg ctggaagaag cgcaaaagcg tggcgcgttg
    39001 ccttacccgg tcggtgcaga gtcgaaatat gaaatcccgc cgctcttcta tcgactttca
    39061 ggaacgtttc gccaggcgaa tccacaactc gaacattgtg cgattcgcat taacccgaat
    39121 cgcagcggtg aagagacggt gctgcggatt ttgcgtgaga gtattgcgag tatttaatgc
    39181 tggatagcac cgttcggcaa tcaagctaaa ttccgcactg aacggtcccc tcgccccttt
    39241 ggggagaggg ttagggtgag gggaacgtgc gggatgtaaa attcagtgcg ccagcatcgc
    39301 cggatgcgat gctggcgcat cttatccggc ctacggttaa tgtcttttat tatcaatcgt
    39361 taaggtgatg cgattggcgc ggcagaagag ttcggagacg tgcaccggtt tgtcattctg
    39421 atcaaaggcg attttgctga tgcgaaacaa cggttcgccc agttcgcatt tcagccattt
    39481 tgcctgcggg cgggtggcgg caaagatatc gatggtcttt ttgtcgctga ccactcgcgt
    39541 gtcaaaacgc tcctgaaata actgataggt ggagcttcct tcgacgtaaa tctcatcaaa
    39601 gtcaggataa cgcgacagcg ggatccatga actatcaata aacagcggct ctttatcgag
    39661 atacatcacc cggcagagat ggaacacttc gctgtttccg gggatgttca gcttttcgca
    39721 aaacggcgcg gcgctgaccc gttcctgttc gatcactttc tctttcgtcg acttgccttg
    39781 tgagacgcca aaatcggtaa aaccactgac agtaagcagg gcgttttcaa ctttctggct
    39841 ttgtacaaag gtgccttttc cctgccagcg gatcagtacg ccgtctgcca ctaagtcgct
    39901 gatggctttg cgaatggtaa tgcggctgac gttatattgt gtacaaagct cgttttcggt
    39961 agggatctgt tgcccggcct ggtaaacccc ctgcgcgata tcatccagca gccgctggcg
    40021 gacggtagcg tagaggagtt gatgagagta gcggtccgta gctgacataa tcgaccctga
    40081 aataaagaat gaattactcg gattataggg tcgttttccg gcaagtaaca gcacgttggc
    40141 gtgaagatgc tcacgccaac gttataccta ccaggcaccg tggtactgaa tggttttcgc
    40201 cgcgcacgcc gttccctgcg ccatcgcctg cggtaatgtc atccccgcag accagccgca
    40261 aaggaatccg gcaatgaacg aatctccggc acccatggtg tcgataaccg tcaccggttc
    40321 aggagcctga cgccagaact gcgcgccatc ccaggcaatg ctgccgtttt cacccagcgt
    40381 gacaatcact gttcctgcgc cacgggcaac aatcgctttc atcttcagac gcagcgcttc
    40441 gtcttcttgc ggtgcggagg caaaggcaaa atcgagatgc ggcaccagtg tctgccagag
    40501 cgggctgtcc cacttgtcgg agaagtcgaa agcggtaagt ttgcccgcag cgtgcagctg
    40561 tgggaatgcg tcttccgcat gtccccagat tgccgcgtgc acaatgtcat actgcgccag
    40621 ccaggcgtaa tcctcttcac tcagggcaaa gtcggccatc acgccttcgg tgtagtcgcc
    40681 aaaaacgcga tcattgtcgt gcagttccac ctgagtttgt gcggtaacgc cgtgtttcgt
    40741 atggacatgg ctgatatcga cgcccatgcg ggcgagatcc tgcttcagct ttgtgccgta
    40801 gtcatcgtca cccacccagg taatgcatcc cggctgtatg ccgtagcgag tgcagtacac
    40861 cgccacattg accgcattac cgccagaaaa cgctttattc agttgcgggt agatatcgac
    40921 gcagttatcg ccgattgtcg ccagggtttt catctctcat cctccggcag cagcgcgcga
    40981 aagcgttcca gcgcctggcg ggcatagagt ctgggctcgt tcatatacat cgtcaccagc
    41041 tccaccgtag agtaaccctc atagccccga tcaataatat cgcgcatcag ttcccgcagc
    41101 ggcattttgc cttctccagg aatgtaatgc gtgtcgctgg ccccgtcgct gtcgacaata
    41161 tgcagatgac gtaatttatc gcccagtttg tcgaaataac tcatcaccgg ttccgcctgg
    41221 acatacggcg cgcaaatgtc gaccatgctg aacaggcgcg gcgaaggcac cagcgccagc
    41281 gcatgaagca catcattagc attacacacg acgttcgatt catacggcgt taacggttcg
    41341 aggatcagat ccatgccgat gttttccgcg tactcacaca gttcgcttaa gttctctgcc
    41401 agccgccccc agataacgtt aggcggcgtg agatagcccg cgtgggccgc agaaatcagc
    41461 gtataacccg cgttcatctc ttttgccata tctatcgcca gcttgatcat gtccaggctt
    41521 tcacgacgca tatgttcatc gcccagcatc atgttatacg gatagccgtt ggtttctggc
    41581 gtgtaaccga taatcggcat ctgatacgtc tgcgccagcg ccttgatttg tttgatgccg
    41641 cccgctttta agtccggcgc gaacgcgtgc gggcgaccgc cccaaatttc gatgccgtcg
    41701 taacccagct cgcttgcatc acgaaatgcg tgttcaatag gcagccgctg gtggccgcag
    41761 gtaaacatac ctgttttcat cgggtaactc cttgcgtaaa tccggcccgt tgccgggccg
    41821 ggagagggat taatattcca ccagaccacc gtaatagcgg cgttcatccg ggttgtgatc
    41881 tttgtaaata gacaggtagt agcagagcca ctccattggc acgaacatca ggaacggtgc
    41941 cagccacggg tgcagccctt gcgaaatttc ggcgtaatcg atgacgatca cgttgtcagt
    42001 acgctgttta acaaagttaa tggcgcgttc ggtggtgtgg cgactttcat cattgccgag
    42061 caggaacagg aacggaacgc ccggttcgac aatctccagc gggccatggc ggaactctcc
    42121 gctctcaatc acgcagccgt gcgtccaggt aaattccatc agcgttacaa tgccttcttt
    42181 gtaacccagc ggacgcagcg gacccgcagc aacggtataa atcatcggcc actggctggc
    42241 cagttcacca agctggcggc ctttttcttc ccaggtgcgt accagatgac cgagcgcatt
    42301 cggcaactgc ttgagatcgt ttttgatctt gccgatttcc gcgttcggcg cgaggcgggt
    42361 gatcatctcc agcaccacgc tgtagcagag cagcaggtga atttcccaga tacagtcggc
    42421 ctgataatca atgctaaatt ccgccgccga ggtaatcggg ctgtccgcgc gtttggtgaa
    42481 cgccgcagtg agtgctccgc aggcccggcc cagctccagc gctttgatta cctcttccgt
    42541 tttaccgtag tcagaaacgc caatcaccgc gcaacgatcg tcgaggcgat acggggtgtt
    42601 atcgcagaac tcccagccgg aaatggcgta gacctgaaga tcggaaaagc gatccgccag
    42661 atgtttcgcc gtttgcgccg cgttgagtgg cgagccgcag gcaacgaaat aaatacgatc
    42721 aatgtcgcgc ttcaccatct cttccacgat ggcgtgcacc agcggaacgt catggctgag
    42781 aactttttcc acttcctgaa ccatattttc ggtgaccaga aagtccacgg tgcttttatc
    42841 aatatccaac attttatttt cttcagataa cattacgaca aagcgttgag ctggcggcta
    42901 cgcttcgccc agaaggcgta agcaggcaga ccagtagcaa taacgatggc ggcgcagata
    42961 aggccgggaa tcggtgccca gacaaaggtt gaggcgacca gaatgaggct tgacgcaatg
    43021 gcgagggtgg tcatcagccc gaaagccgga gtacgccaca gcggtttgta atcgtcgcgt
    43081 ttacgacacc agatgatgga gccgaaggtg agggtatttt tgaaacacat caccagggtg
    43141 aaataaccca gcaggctggt gagatcggaa acgaagatga agaagatccc cagcgcccct
    43201 tgcaggatga tggagacatc cggcgtgttg tatttcggat gcgcatggcc gaagcattta
    43261 aagaacaggt tgtctttcgc catcgcgtat tccagccgcg gctggtacat cacgcagctg
    43321 gaaagcgaac caagaatgac gatcatcgcc gtgatggcaa caaagagccc agcggtgctg
    43381 ccgagtgcgg ggatctaggt cagggcgtcg gaaatcggcg tttcagaatt ggcgagtttg
    43441 tcgaagggca tcaggccgga aatcaccagc gccagcaggg tgtagagcac cagaaccagc
    43501 agacaggaac caatcagcgc tcgtggcatg gtttttccgg ggtttttaat ttcgccggtc
    43561 atataacaga tagaggccat gccggtatac gaccaactgg tggcagagat ccccgccagc
    43621 agcgccataa agctgcccgt tgcgccaatc gcagtggtgg taagggcggc aaaattctcc
    43681 gctttaaacc agaagatccc aaggccaatg acgatagtga acgggataat tttggcgatg
    43741 gtaattagcg tctgaaacgc tgcgccgcct tcaacggagc gcaggtgtag caacataaag
    43801 gcgataatta atccggcggc gataaattta ccgagcaacg gatcgatagg cgttaaaaag
    43861 ccaagattgc tgacaatcgc cagcgccata atcgacaatg acggcgcatc gttggcccag
    43921 aagctggccc agccggagag gaaagccagc ggtcggcttc cggcattttt cagataaaca
    43981 taatctgcgc cattttccgg ataagcggtg gatagttccg catagacgca catttgcggg
    44041 atcacaatta acccgccaat gacaaacgcg agcaccgtaa gccacggcgt gcccgctgct
    44101 tttgccactt cacccacaga tacaaaaata ccggagccga cggttgtccc gacggcgatt
    44161 gcaagaacgg cccaaaatcc gagcttgcgt tggagttcct ggcttcccat agcgatacct
    44221 ttttcttgtc caatacgttg taggggagag tttgcagcca tttaaaaggt aacatgacaa
    44281 tgcatgatga atataacaac atattatgtt atgtgatgtg gaggaagatc acaaaatttc
    44341 gcacaggatc gcgctgtggc taatggatgt agttataaaa ttgaatttaa agtgaaaata
    44401 tttttacggg cgggggcaag aaggacatag aaacaaatac gccctcggaa aatccagagg
    44461 gcgtcgggca attaaaccgg tgttagccga tttctgtcag agacttactg tgcagtagga
    44521 ccgcaaccgc caatgatttt agaaatagaa attgccgggt gcagcaggta gtcgtaagag
    44581 cagttcttat cacggttttc aatatgacca gtacatgcag ttaacgtagc cagtactgca
    44641 cctaccagtg caactttaat aaa
//
LOCUS       DAZPDE010000017        42340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPDE010000017 DAZPDE010000000
VERSION     DAZPDE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.017"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..586
                     /locus_tag="ACHU0J_000825"
     CDS             <1..586
                     /locus_tag="ACHU0J_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215635.1"
                     /translation="SNQPLEDWDIIISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQK
                     SHSESEMRGLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQYFINAKEWNNARE
                     LLIKEEMLGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAITKIDSVGNEQLYQI
                     GKIYHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(646..1137)
                     /gene="yajQ"
                     /locus_tag="ACHU0J_000826"
     CDS             complement(646..1137)
                     /gene="yajQ"
                     /locus_tag="ACHU0J_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1215636.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1305..2216
                     /gene="panE"
                     /locus_tag="ACHU0J_000827"
     CDS             1305..2216
                     /gene="panE"
                     /locus_tag="ACHU0J_000827"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1215637.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2179..2769
                     /gene="yajL"
                     /locus_tag="ACHU0J_000828"
     CDS             2179..2769
                     /gene="yajL"
                     /locus_tag="ACHU0J_000828"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1215638.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2823..4271)
                     /gene="thiI"
                     /locus_tag="ACHU0J_000829"
     CDS             complement(2823..4271)
                     /gene="thiI"
                     /locus_tag="ACHU0J_000829"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1215639.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4477..4719
                     /gene="xseB"
                     /locus_tag="ACHU0J_000830"
     CDS             4477..4719
                     /gene="xseB"
                     /locus_tag="ACHU0J_000830"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1215640.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4719..5618
                     /gene="ispA"
                     /locus_tag="ACHU0J_000831"
     CDS             4719..5618
                     /gene="ispA"
                     /locus_tag="ACHU0J_000831"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1215641.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5643..7505
                     /gene="dxs"
                     /locus_tag="ACHU0J_000832"
     CDS             5643..7505
                     /gene="dxs"
                     /locus_tag="ACHU0J_000832"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1215642.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7560..8477
                     /gene="yajO"
                     /locus_tag="ACHU0J_000833"
     CDS             7560..8477
                     /gene="yajO"
                     /locus_tag="ACHU0J_000833"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1215643.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8588..9106)
                     /gene="pgpA"
                     /locus_tag="ACHU0J_000834"
     CDS             complement(8588..9106)
                     /gene="pgpA"
                     /locus_tag="ACHU0J_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1215644.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9084..10061)
                     /gene="thiL"
                     /locus_tag="ACHU0J_000835"
     CDS             complement(9084..10061)
                     /gene="thiL"
                     /locus_tag="ACHU0J_000835"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1215645.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10139..10558)
                     /gene="nusB"
                     /locus_tag="ACHU0J_000836"
     CDS             complement(10139..10558)
                     /gene="nusB"
                     /locus_tag="ACHU0J_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1215646.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10578..11048)
                     /gene="ribE"
                     /locus_tag="ACHU0J_000837"
     CDS             complement(10578..11048)
                     /gene="ribE"
                     /locus_tag="ACHU0J_000837"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1215647.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11137..12240)
                     /gene="ribD"
                     /locus_tag="ACHU0J_000838"
     CDS             complement(11137..12240)
                     /gene="ribD"
                     /locus_tag="ACHU0J_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1215648.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12244..12693)
                     /gene="nrdR"
                     /locus_tag="ACHU0J_000839"
     CDS             complement(12244..12693)
                     /gene="nrdR"
                     /locus_tag="ACHU0J_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1215649.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12844..13383
                     /locus_tag="ACHU0J_000840"
     CDS             12844..13383
                     /locus_tag="ACHU0J_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1215650.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13681..14565
                     /gene="tsx"
                     /locus_tag="ACHU0J_000841"
     CDS             13681..14565
                     /gene="tsx"
                     /locus_tag="ACHU0J_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1215651.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14742..15089)
                     /gene="yajD"
                     /locus_tag="ACHU0J_000842"
     CDS             complement(14742..15089)
                     /gene="yajD"
                     /locus_tag="ACHU0J_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1215652.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15218..16189)
                     /gene="secF"
                     /locus_tag="ACHU0J_000843"
     CDS             complement(15218..16189)
                     /gene="secF"
                     /locus_tag="ACHU0J_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1215653.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16200..18047)
                     /gene="secD"
                     /locus_tag="ACHU0J_000844"
     CDS             complement(16200..18047)
                     /gene="secD"
                     /locus_tag="ACHU0J_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1215654.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18075..18407)
                     /gene="yajC"
                     /locus_tag="ACHU0J_000845"
     CDS             complement(18075..18407)
                     /gene="yajC"
                     /locus_tag="ACHU0J_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1215655.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18430..19557)
                     /gene="tgt"
                     /locus_tag="ACHU0J_000846"
     CDS             complement(18430..19557)
                     /gene="tgt"
                     /locus_tag="ACHU0J_000846"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1215656.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19612..20682)
                     /gene="queA"
                     /locus_tag="ACHU0J_000847"
     CDS             complement(19612..20682)
                     /gene="queA"
                     /locus_tag="ACHU0J_000847"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1215657.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20775..21348
                     /gene="acpH"
                     /locus_tag="ACHU0J_000848"
                     /pseudo
     CDS             20775..21348
                     /gene="acpH"
                     /locus_tag="ACHU0J_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21353..23170)
                     /gene="malZ"
                     /locus_tag="ACHU0J_000849"
     CDS             complement(21353..23170)
                     /gene="malZ"
                     /locus_tag="ACHU0J_000849"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1215658.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23326..24699)
                     /gene="proY"
                     /locus_tag="ACHU0J_000850"
     CDS             complement(23326..24699)
                     /gene="proY"
                     /locus_tag="ACHU0J_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1215659.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24775..26094)
                     /gene="brnQ"
                     /locus_tag="ACHU0J_000851"
     CDS             complement(24775..26094)
                     /gene="brnQ"
                     /locus_tag="ACHU0J_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1215660.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26501..27796)
                     /gene="phoR"
                     /locus_tag="ACHU0J_000852"
     CDS             complement(26501..27796)
                     /gene="phoR"
                     /locus_tag="ACHU0J_000852"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1215661.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27854..28543)
                     /gene="phoB"
                     /locus_tag="ACHU0J_000853"
     CDS             complement(27854..28543)
                     /gene="phoB"
                     /locus_tag="ACHU0J_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1215662.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28733..29935
                     /gene="sbcD"
                     /locus_tag="ACHU0J_000854"
     CDS             28733..29935
                     /gene="sbcD"
                     /locus_tag="ACHU0J_000854"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1215663.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            29932..33075
                     /gene="sbcC"
                     /locus_tag="ACHU0J_000855"
     CDS             29932..33075
                     /gene="sbcC"
                     /locus_tag="ACHU0J_000855"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1215664.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33201..34382
                     /gene="araJ"
                     /locus_tag="ACHU0J_000856"
     CDS             33201..34382
                     /gene="araJ"
                     /locus_tag="ACHU0J_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1215665.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34525..35433)
                     /gene="mak"
                     /locus_tag="ACHU0J_000857"
     CDS             complement(34525..35433)
                     /gene="mak"
                     /locus_tag="ACHU0J_000857"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1215666.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35558..36469
                     /gene="rdgC"
                     /locus_tag="ACHU0J_000858"
     CDS             35558..36469
                     /gene="rdgC"
                     /locus_tag="ACHU0J_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1215667.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36547..37002)
                     /locus_tag="ACHU0J_000859"
     CDS             complement(36547..37002)
                     /locus_tag="ACHU0J_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215668.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(36999..37334)
                     /locus_tag="ACHU0J_000860"
                     /pseudo
     CDS             complement(36999..37334)
                     /locus_tag="ACHU0J_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37489..37773)
                     /gene="ppnP"
                     /locus_tag="ACHU0J_000861"
     CDS             complement(37489..37773)
                     /gene="ppnP"
                     /locus_tag="ACHU0J_000861"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1215669.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(37845..38522)
                     /locus_tag="ACHU0J_000862"
     CDS             complement(37845..38522)
                     /locus_tag="ACHU0J_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1215670.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38780..38971)
                     /gene="yaiA"
                     /locus_tag="ACHU0J_000863"
     CDS             complement(38780..38971)
                     /gene="yaiA"
                     /locus_tag="ACHU0J_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1215671.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39021..39545)
                     /gene="aroL"
                     /locus_tag="ACHU0J_000864"
     CDS             complement(39021..39545)
                     /gene="aroL"
                     /locus_tag="ACHU0J_000864"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1215672.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39728..40186)
                     /locus_tag="ACHU0J_000865"
     CDS             complement(39728..40186)
                     /locus_tag="ACHU0J_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1215673.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40306..41115
                     /gene="proC"
                     /locus_tag="ACHU0J_000866"
     CDS             40306..41115
                     /gene="proC"
                     /locus_tag="ACHU0J_000866"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1215674.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41132..42247)
                     /gene="adrA"
                     /locus_tag="ACHU0J_000867"
                     /gene_synonym="dgcC"
     CDS             complement(41132..42247)
                     /gene="adrA"
                     /locus_tag="ACHU0J_000867"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1215675.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10650 a        11328 c        10574 g         9788 t
ORIGIN      
        1 cagtaatcaa ccattagaag actgggatat aataataagt aatactggtg aaaaatttag
       61 tgaagtgggt ctaaccaaat atgctcaaca tctcgcatct ttcgctgaag aattttatat
      121 aaaagagcaa aaatcacatt ctgaaagtga aatgagagga ctattttcat cacttgccga
      181 attatatagt tttggtaatg atactgccag cgcaaaccga gtgttccatc agcatgtccc
      241 tgccttcaat acagatcata tgattcaata ttttataaat gccaaagaat ggaacaatgc
      301 cagggaatta ttgataaaag aggaaatgtt gggtatgcat aatctgatat tgttggagaa
      361 tatttgtatg caaaaaaatg cagaatgcat ggcacacatt acctttacgc ttaataaatt
      421 aacaacacaa ccagcaataa caaaaataga tagcgtgggt aatgagcaac tttatcagat
      481 tggcaaaatc taccataagt tggaaattaa acctgagcca gagcaacagg ttttaattca
      541 gtcgctttat gaccgggcat caggaagcgc ctctgcaact caataaatgc atggtcaaaa
      601 gggccaccac ggtggccctg acaatcagta gctatatcca gaaagttaat cgcggaagtt
      661 tttgaactgg aacggctgac cgagatcgcc accgcgcacc atcgccatga cagcctgcaa
      721 atcatcacga gatttgccag tcacgcggat ctcatcgccc tgaatttgcg cctgcacttt
      781 cagtttgctg tctttgatca tcttgacgat ttttttctga gtggcgctct caatgccctg
      841 tttcagtttc gcttccacaa accaggtttt accgctatga acgatatttt ccggtacatc
      901 cagtgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
      961 attgacctgg aagtcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     1021 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcgac tcgcgttatc
     1081 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     1141 ttcctctccc ttcatttttg atgcgaagca taatacctgc aaagttaaat aaccaacagt
     1201 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     1261 tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat
     1321 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgtaaacag ggtcatgaag
     1381 ttcagggctg gctgcgtgta ccgcaacctt attgtagtgt gaatctggtt gagacagatg
     1441 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttctcgcc accagcgatc
     1501 tgctcctggt gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc ctcgcatcca
     1561 cactgcctgt aactacgcca atactgttaa ttcacaacgg catgggcacc atcgaagaat
     1621 tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     1681 gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat cggtccggca cggcaacagg
     1741 atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac gttgcctggc
     1801 ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgcgtg attaatccac
     1861 tgactgccat ctggaattgc ccgaacggtg aattacgtca tcatccgcaa gaaattatgc
     1921 aaatatgcga agaagtcgcg gcagtgatcg aacgcgaagg gcatcatact tcagcggaag
     1981 atttgcgtga ttacgtgatg caggtgattg atgccacagc agaaaatatc tcgtcgatgt
     2041 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     2101 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     2161 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     2221 gagactgaag ccgtcaccac gatcaacctc ttggttcgcg gcggtatcaa agtcaccact
     2281 gccagcgtcg acagcgatgg caacctggca attacctgct cgcgcggcgt gaagctgctg
     2341 gcggatgcgc cgctggtcga agtggctgat ggcgaatatg acgtgatcgt gctgcctggt
     2401 ggcattaaag gcacggagtg ttttcgcgat agcactctgc tggttgaaac cgttaaacag
     2461 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg
     2521 ccgcacgata tctttccgat tggcaatatg accggcttcc cgacgctgaa agacaaaatt
     2581 cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     2641 agccaggggc cgggtacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg
     2701 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     2761 tacgagtagt gtcggatgcg gcaaacgtcg catctgacca gatgcgacgt aaaaactaca
     2821 aattacgggc gatatacctt cacattgtta aagccttgct cgcgcagata gagcgcctgc
     2881 agacggctca tcaccccgcg ctcacaccac agcagccagg ttttgttctg gtcgagatcg
     2941 ccaaatttgg tgctcagttt atagaacggc agagaaacca catcaatccc ttcgactttc
     3001 agtggcttat cttcctgttc atcgacagaa cggatatcga gaatcacgtc gttcgggccg
     3061 aagccgttga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     3121 atatcaacgt tattcgcttc ctcaaccact ttatcgagaa tactgaagtc gaacttctct
     3181 tcttccgctt caatcttcga tttaaccgct ttcaccgtcg ggcttttgga gatcacgcca
     3241 caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc caggttgatg
     3301 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtgtcaga gacgttatca
     3361 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     3421 agcgcctgta cgccgtaacg ttcagccact ttagatgcgg cacgcaccat catacgtttg
     3481 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac aaccggttcg
     3541 aaattaatgg cgacaaaacg cacgcggtgg gagctgccaa agcggttcca cagataatgc
     3601 gccacctgac gcacgccaat ttcatgcgct gcgccgccga ggttaaagaa gcagtaatgc
     3661 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     3721 atgagcgaca gcacatcttc ctgggtgcca atcgggaaac caccaatacc ttcgtagcga
     3781 cctttaatca gcaggaggcg atcgtcttcc acttccagat gaacagttac atccgggtta
     3841 gtcagcttca cgcgcgcgga ttcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     3901 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     3961 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     4021 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     4081 cgaatagtca gacgctggtt ttcatctttc gcgcgaactt cgatgttatc ccagtggcgg
     4141 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     4201 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     4261 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     4321 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     4381 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     4441 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggcgccc
     4501 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     4561 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     4621 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     4681 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg
     4741 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     4801 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgcctgc
     4861 gacctttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac acgctggacg
     4921 cacccgctgc tgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     4981 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     5041 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     5101 ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     5161 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     5221 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     5281 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     5341 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     5401 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     5461 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     5521 acgatgcccg tcaggcgctg aaacaactgg ctgaacagtc actcgatacc tcggcactgg
     5581 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     5641 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     5701 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     5761 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     5821 ccgtggcgct gcactatgtc tacaacaccc catttgacca attgatttgg gatgtgggac
     5881 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     5941 agaaaggcgg tctgcacccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     6001 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     6061 aaggcaaaaa tcgccgcacc gtctgtgtga ttggcgacgg cgcgattacc gctggcatgg
     6121 cgtttgaagc gatgaatcac gcgggcgata ttcgtcctga tatgctggtg gtcctcaacg
     6181 acaatgaaat gtcgatttcc gaaaatgttg gcgcgcttaa caaccatctg gctcagctgc
     6241 tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc tctggcgtgc
     6301 cgcccattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtggtgcctg
     6361 gcacgttgtt tgaagagctg ggatttaact acatcggccc ggtggacggt cacgatgtgc
     6421 tggggcttat caccacgcta aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     6481 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agacccgatc actttccacg
     6541 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     6601 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     6661 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     6721 cggatcgtta cttcgacgtg gctattgccg agcaacacgc agtgaccttt gccgccggtc
     6781 tggcgattgg tggatacaaa cccattgtcg cgatctactc caccttcctg caacgcgcct
     6841 atgatcaggt gctgcatgac gtggcaattc aaaagctacc ggtcctgttc gccattgacc
     6901 gcgcgggcat tgttggtgct gacggtcaaa ctcaccaggg cgcttttgat ctctcttacc
     6961 tgcgctgcat accagaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     7021 tgctctatac cggctatcac tataacgatg gcccgtccgc ggtgcgctac ccgcgcggca
     7081 acgcggttgg cgtggaactg acgccgctgg aaaaactacc aattggcaaa ggcattgtga
     7141 agcgtcgtgg cgagaaactg gtgatcctta actttggtac gctgatgcca gaaacggcca
     7201 aagtcgccga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccacttgatg
     7261 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     7321 ccattatggg cggcgcaggt agcggagtga acgaagtgct gatggcccat cgtaaaccag
     7381 tacccgtgct gaacattggc ctgccggact tctttattcc gcaaggaact caggaagaaa
     7441 tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggttgg
     7501 cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca
     7561 tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta
     7621 tgacctttgg cgagccagat cgcggtaatc acgcatggac actgccggaa gagagcagcc
     7681 gtcccataat taaacgcgcg ctggaaggtg gcataaattt ctttgatacc gccaatagct
     7741 attccgatgg tagcagcgaa gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg
     7801 aagacgtagt cgttgccacc aaagtgttcc atcgcgttgg tgatttaccg gaaggattat
     7861 cccgtgcaca aattttgcgc tctatcaacg acagcctgcg acgtctcggc atggattatg
     7921 tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagag acgctggaag
     7981 ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tccatgcacg
     8041 cttcgcagtt tgctcaggcg ctggaactac aaaaacagca ctgctgggcg cagtttgtca
     8101 gtatgcagga tcactataat ctgatttatc gcgaagaaga gcgcgagatg ctgcctctgt
     8161 gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg cgactgacgc
     8221 gtccatgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa aatctctata
     8281 aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac
     8341 tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt aagtaaaccg ggcattgccg
     8401 caccgattat cggaacttcg cgggaagaac agcttgatga gctgctgaac gcggtggata
     8461 tcactttgaa gccataacag attgccgagc tggaaacgcc gtataaaccg catgcggtcg
     8521 taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat caggcatcgg
     8581 tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac aggatgcctg
     8641 cggaaatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca tgtacattgc
     8701 gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa atcacaaacc
     8761 cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc cacataccaa
     8821 taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc gccgtttgat
     8881 gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag agctgccagg
     8941 gtaaaaaggt catcagatac cagaaaggga tcgccgccag cgatcccatc gagccaggaa
     9001 cgattgggct taatccactt ccgaatccga cagcaagcag atgccacgga ttactcatct
     9061 tcaggcgact tttcgcgaca tctttatggc gtgacaaaat ggtcatatcc tttccagtca
     9121 aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc ggtcatttgc
     9181 ccgatacagg taaacggtac gccaaggtgt ccgagagcaa catccagcgc gccacggttc
     9241 agttccggca ccgtgaaaca caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc
     9301 gcctgttccg gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca
     9361 atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat cagaccgtca
     9421 gagagatcga tagctgaatt tgccagatcg cgcagtgcct gcccctgtaa aatacgcggc
     9481 gatggacgga gatgacgttt gatcaagtag tcagcatctt tagcatcggc aacctgcaaa
     9541 cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt cacatagatc
     9601 cagtcacccg gtttcgcccc agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg
     9661 atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg
     9721 taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc ttcgtctacg
     9781 tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc tgccagatcg
     9841 cttaggttca ccgccagtgc tttataagcc agatcagcag gatcgatatc agggaggaaa
     9901 tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc ggggatattg
     9961 agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact tcttacacgg
    10021 tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc ctcagcaggt
    10081 aaaaaagaaa aggccggcga tcatggaaca gtcttccgtg aatctaccgg cctggatatt
    10141 actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg acgaacttat
    10201 ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg gccactttgt
    10261 atggcacatc actacgttta gacagttcgt acagcgcaat gcgcagtact gctttttcta
    10321 cctggcccag ttcttccagc aggcgggaca ggtagggctt catcagtccg tcgaggtatg
    10381 cggtgttagt cgccaccccg gccagcagct cacggaagta caggacgtca acgtctttta
    10441 catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg gacaactgcc
    10501 aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca ggtttcacgg
    10561 atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat ttcaagcgcg
    10621 gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc gatcgcttgt
    10681 tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca
    10741 tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt gccaccacga
    10801 ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag tgcacccgcc
    10861 gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc atcttttacc
    10921 tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt gataaagttg
    10981 ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata
    11041 atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga ttttagcata
    11101 atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta aatgcaggca
    11161 aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt
    11221 ctcaagccct ggcagcgagc ataatccacg ggcgtcgctg cctaatagtt taggtgcgat
    11281 atagacaatc agctcatcga ctaaacccgc ctgtagcaat acgccagcga gcgttggccc
    11341 cgcttccacc cagatgctgt taatctgctg tttacccagt tgcatcatca gtacaaccag
    11401 atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg gccactcacg
    11461 agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc
    11521 cggcgtcacg cgattttggc tatcaatcac aatacgtact ggctggcgga gattttgttg
    11581 cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta aggctggatc
    11641 atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc gctgtcgttg
    11701 tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt
    11761 gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg
    11821 cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt ggctgacgtc
    11881 aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg
    11941 catcgctgcg accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt
    12001 acggccatga tggctacagg gttcgagtgt cacatacgcc gttgcgcctt tggctttttc
    12061 acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgctcgtt ggtggtaacc
    12121 ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt tggggtgggt
    12181 ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcac
    12241 ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt cgaaactgcg
    12301 gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt gctccatcac
    12361 cagattgcca atcatcttgc tcggtacttc gcgctcaccg gtggcgcgca gctgcgattt
    12421 aatatgattg attgccattt caacgtaatc ggaactcacc ggacgttttt ccagcgcccg
    12481 cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt tgctttttac
    12541 aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat tacacaccag
    12601 acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt caattacctt
    12661 agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg tggttaacgg
    12721 aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg agataaagga
    12781 tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag aaagggacga
    12841 cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc
    12901 tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg tcagcacgct gaaccaggag
    12961 atgacgcaac tcaatcaaga aacagtcaaa atcactcagc aaaacaggct gaatgcaaaa
    13021 tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact ggaaagccag
    13081 atcggtactt tacgtatgtc gctggtgaat attacgcctg atacagatgg caccaccctt
    13141 acactacgta ttcagggtga gtcgaatgat cccttgcccg cattcagcgg aactgttgaa
    13201 tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca gaatcaattg
    13261 attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt acaattaaag
    13321 gggatttctg tggatcagtt aggctttgta cgtatccacg acattcaacc tgttatgcag
    13381 taaacgtatt tcgggacgat tttgtgcgtc ccgcaacatc ttccccgtca ttttgttact
    13441 ctgcttacat cacctggatt gatagtaaaa gtttgcaaca agggcgaaag tcagtacaat
    13501 ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata gaactgtgaa
    13561 acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat acgaaatatt
    13621 tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag tggcatacat
    13681 atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc ttttactgtc
    13741 aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca gagcgttaac
    13801 gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac ctaccttgag
    13861 tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga tgcgccggta
    13921 ttcttcggcg gtaactccga tgctaaaggt atctggaacc acggttctcc gctgtttatg
    13981 gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag cttcggtccg
    14041 ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa taaagatggt
    14101 cgccagagca cctggtacat gggtctgggt accgatatcg acactggcct gccgatgagc
    14161 ctgtccatga acgtctatgc gaaataccag tggcagaact atggcgcagc gaacgaaaac
    14221 gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga tctgtggggc
    14281 ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt aggggatgac
    14341 agcggtaacg caatcaacgg tattaagacc cgtactaata actctatcgc ttccagccat
    14401 attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta ctggcacgac
    14461 ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt caacgttcgc
    14521 tctaccggct ggggtggtta cctggtagta ggttacaact tctgattatg aaaatgccgg
    14581 gatttattcc cggcatttct gattgttaac aacgtgtgct ttgttcatgc cggatgcggc
    14641 gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa tatattgctg aaataacgta
    14701 ggcctgataa gcgtagcgca tcaggccatt ttacgtttta atcacttctt cttgttcatc
    14761 atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt ctgcgcatct
    14821 tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga atgctcatga
    14881 tcgtggcaat agagacacaa caattcccag ttactgccat cttccgggtt attggtatgg
    14941 tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttag aataaacaaa ctcgcgggaa
    15001 cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc gctttctaac
    15061 cgtgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat gtagggttac
    15121 gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa aaatcccgat
    15181 cttctgaccg ggattttcaa catcaacggg aacttgatta aggcagaatt gacggctgat
    15241 ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata cccagtttca
    15301 gagccaacgc agatgctacg tagatggaag atgcagtgcc gatggaaaca ccgataagca
    15361 tggtcagcga gaagccttcc agtaccggac caccgaagag atacagcatc aggataacca
    15421 tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa
    15481 agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaatt cggtccgata
    15541 ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc acaatggtca
    15601 ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg tcgtgcgtca
    15661 gcgcaataac cacccctgcc gccagtcgcc actcaaagcg gaaacctacg tacacgagga
    15721 tagacagcag cgctgccatc aacgccatcg caccggtttg cgcaaggtca gcccccacgc
    15781 tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat tcgttaatca
    15841 ccttcagaac ttggctgccc agcacctgac cgccggtttc gccttcagca ggcggcatac
    15901 ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca aaaccggctt
    15961 tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta atttcgataa
    16021 ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg cacgcccata ataacgatag
    16081 cagcgattaa cagcagacca gagatgccga aagcccagta gtcccagcgc ataaagtcat
    16141 agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca tcgcactcct
    16201 cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat ggcacgcgta
    16261 ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct
    16321 ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt ggtgatgttc
    16381 gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg ttgaacagta
    16441 cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc atcgaccgct
    16501 accgcaaggg ttaagacgat acccgcgata cctggcatac tcagtgttgc gcctggcaac
    16561 agcgacataa taccgacgat caagatcaag ttggcaatca gagcactggt cgcaatcaga
    16621 ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa
    16681 gcctccagcc cctgctcaat gttctgcata cccagggtcg ggccaattgt acgttcttca
    16741 acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag ctgacgggct
    16801 tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga ctggatgttg
    16861 gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc atctttctta
    16921 ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt gtccttggtg
    16981 aagttagaca taatgttgcc accagcgcta tcgagcgaga tgttaacctg cggctggttg
    17041 tattcgtcct ggctggaagt ggagtcggtg atatggtcac cagtcagaat tacgcgtttg
    17101 tacagcacaa ccggctgacc ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga
    17161 ccggatgccg cagcggcctg atcaacattg gtgtttacca gacggaattc cagcgttgcc
    17221 gtcgcgccca gaatctcttt cgcacgcgca gtgtcctgaa taccgggcag ttcaacaacg
    17281 atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg gtttacacgg
    17341 ttacgcagga tattaatgtt ctgctgcacc gcatattcac gcgcttcact cagacgagca
    17401 tcgctcatta ccgcacgcag ctggttgctg ccctggctgc taatcaccag gtccggatgg
    17461 cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa agtgatgctc
    17521 aggccgtagt tgttttcttt acgaacagtg gtatacggga tgcccttttc gcgcaggtca
    17581 ctgcgtaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc catatccact
    17641 tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg ctcagcatga
    17701 atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac gtatttgtca
    17761 cccataacgc ccattaatgc ttcacgagcg cgcaactggg tgtcagtgga gtcgaagcgc
    17821 gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc ttcttgtaac
    17881 gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc accagtgatc
    17941 tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc gatgacaatc
    18001 accacgatca gcatgacgta cttccacaaa ggataacggt ttaacacggc aattccctta
    18061 gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc agctacgaag
    18121 tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta gccgttttcc
    18181 gctactttgg ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc acctttggca
    18241 atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg caggatcatg
    18301 aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg gctaccttgc
    18361 gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa atttccctca
    18421 ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc
    18481 agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg ccatcaaacg
    18541 ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg
    18601 gtcgagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat cacactcagg
    18661 atcgagtggg ccagtatcgc tcttatattt cgcattgcgg attttcacca cgccatcggt
    18721 cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa acatatcgat
    18781 accgcgacgt acgccttcaa gcaggtcttc cggtttacca acgcccatca ggtaacgcgg
    18841 tttgtctgcc ggaatttgcg gacacacatg ctccagaata cggtgcatat ctgctttcgg
    18901 ctcacccaca gccagaccgc cgacagcgta gccatcaaag ccgatatcta ccagaccttt
    18961 aacagatata tcacgtaaat cttcgtaaat actgccctga attataccaa ataacgcatt
    19021 tttgttacca agactgtcaa aacgctcacg gctacgcttc gcccaacgca gagacatctc
    19081 catagagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat caaagatcat
    19141 gacgatatcc gaaccaagat cgtactgaat ctccatcgac ttttccgggt cgaggaaaat
    19201 cgggtcgccg ttgatcgggt tacggaagtg aacgccctgt tcggtgattt tacgaatatc
    19261 accaaggctg aagacctgga agccgccgga gtcggtaaga atcggtcctt tccactgcat
    19321 aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca gccacaggtg
    19381 gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg gcgtcatccc
    19441 ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta cgccacgatc
    19501 aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt
    19561 ttctccacta cgtcagaaaa acagtccaac gtttaaacca gcgccgcgga attactcccc
    19621 gacgcgctca ttaattgcct gcggattgta cgtgataaac atcgcatcac cgtaactaaa
    19681 aaagcgatat ttctcttcta ccgccgcttt ataggcattc atggtgtgtt gataaccggc
    19741 aaaggccgaa accagcataa tcagcgtcga ctctggcaag tggaagttcg tcaccagcgc
    19801 atcgaccact ttgtactgga agcccggata gataaagatt tgggtatcgt caaagaacgg
    19861 ttcgataaga tcgttttttg ccgcctgtgc cgcgctttcc agcgaacgta ctgaagtggt
    19921 gccaaccgca atcacccggt taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc
    19981 ctgcggtact tcagcgtatt ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac
    20041 cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac gccatctcca cgcctttggc
    20101 gcgtaatttt tccagcaaag gctcgtcaaa atgcagacca gcggtcgggg ctgcaaccgc
    20161 gcccggtttt tcgctataaa cggtttgata aagttcgcgg tcagcgtctt cgtccggacg
    20221 atcgatatac ggcggcagcg gcatatggcc gatgctgttg aggatatcca gtaccgagcg
    20281 ttcatcatta aattcgactt caaacagtgc gccgtggcgc gcggtcattg ttgcgttgat
    20341 actttcgtca tcgcccagca acagttctgc gccaggtttt ggcgctttcg aggcgcgaat
    20401 atgcgcaaga atgcgtttgt cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc
    20461 gctggcttta cgcccaaaca gacgcgccgg gatcacgcgg gtattattaa aaaccagaag
    20521 atcgccgggg ttgagcttat cgagtaaatc ggtgaaagta ccgtgcgtca gcgcgcccgt
    20581 cggcccgtcc agcgacagta aacgacagct actgcgttca ggcatgggat agtgggcaat
    20641 cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc atgacactga ctcagactaa
    20701 aataagagtc gggtagtcta gtgccggggc gctctccctg caacctttac ccccctttgg
    20761 atagaatata atcgatgaat tttttagctc acctgcattt agcccatctc gcggaaagct
    20821 cgctttctgg caatttactg gctgatttcg tacgcggaaa tcctgaagaa agttttccgc
    20881 ccgacgtcgt ggctggcatt catatgcatc gacgtattga ctgacaatct gccggaagtc
    20941 cgcgaagcac aggagtggtt tcgtagtgaa acgcgccgcg ttgcgcctat tacgctggat
    21001 gtcatgtggg atcactttct ttcccgccac tggtcgcagc tgtcgccgga ctttccgcta
    21061 caggaatttg tctgttatgc ccgcgagcaa gtgatgacga ttttgccgga ctcaccgcca
    21121 cgttttatca atctgaacaa ttacttgtgg tcagagcagt ggctggtgcg ctatcgcgat
    21181 atggatttca tccagaacgt gttaaacggc atggcaagcc gccgcccacg tctggatgcc
    21241 ctgcgtgact cctggtacga tttagacgct cattatgacg ccctcgaaac ccgcttctgg
    21301 cagttttatc cgcggatgat ggcgcaggcg tcacacaagg cgttataaaa cgttagttca
    21361 tccataccgt agccgaaatg gcaggcaaag ccagaatccc gtcagtcagt tgcccatgcc
    21421 cttctttgca ttgccattgc acggcattga gaaacggtga cgcgggtagc accacttcac
    21481 tggcctcgcc acggttgatt gccaccagta cacgttgctg attcagcacg cggacaaata
    21541 ccactacgtt atcttccgca tacagcacct gacagccgcc acgacgcagc gcctgacttt
    21601 tcttacgcag cacaatcatt cgccggtaca gcgcgaataa cgccgtatcc tgcttttcca
    21661 cctgccaggg gaacggttta cggcaaaacg gatcgttttt accatccagt cccacttcat
    21721 caccgtaata aatgcacggt acaccaggcc aggtgaacag ccagaccacc gccagcggca
    21781 ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc agtatcgtgg ctatcgagct
    21841 gattaaacat acgtaattgt tgttgatgag aaagccctgc gcggtaatta tccatccagg
    21901 ccatacaggt ttgggcgtca atttgctgcg gatcataaga gatgtcggta ttggcaagaa
    21961 atccccacaa cgggaatgtg aagccacgat agttcatggc agcatcttcc acatcggcct
    22021 gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat atacgcttcc ggctgggttt
    22081 ctttcgccgc ttcggtgatc ccggcaacgt gctgcatatt attgcgcgcc ccacccgcct
    22141 cccccagcat atgcaccaca tccagccgcc agccgtccat attccacggc gctttcagcc
    22201 agtggcggac aatactgtct tccccgcgat aaatttcatt caccagactt tccgactgat
    22261 aatccagctt cgtcaagctg gcatagccaa gccagtcgag cgccgtgcca tcatcactaa
    22321 acgagtacca gtcgcgccag ggcgattcag agttgtgaca agctcccccc gtgccaagat
    22381 tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt aaacacgccg tccagcacca
    22441 gccgcattcc cagctgctgc gtattgtgtc gcaaacgcag caacgcccca tcaccgccaa
    22501 actgcggatc gacatggcga taatcctcgg tatcgtattt atgtacgctg ggagctttaa
    22561 acaccggatt gagatacagc gctgtcacgc caagcttttt cagatacggc agtttttcgc
    22621 ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt
    22681 catcccagtc acgcaagatg atctcttgtc cggctgcatg atggtaatag acatgatcct
    22741 gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg gaagatctga taaaaaatct
    22801 gatccgcagc ccattgtggg ccgatatcag gtacatcgac ggcaaactgc tccagtcgtg
    22861 ccggcggcat tcggctgaag ccctgcggtg taaaccaacg ctggcgatcg tgccacagca
    22921 gtttgaaact gtaacgccgc cggggttgtc cgctggagag atcaatcgcc gcacgccatg
    22981 cggtgacgcc tggctgcggc tgactgcgct gcttatgcat tggtactgac atttcttcgt
    23041 tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc ttcgcccgtc agccacagtg
    23101 taatgagcag ttgatctttg ctttgtttaa caaatggggg caccggcagg tgccatgcat
    23161 ttaacatcat aattcccctg tgatgaaaat ctgatcacct tgccatagcc taatgcagga
    23221 tcactcatca tctgaaggat atttggggga ggaggacggg ggcggataac tttcggaata
    23281 gccggatcag gatttcctcc tgaaccggct tacagaacgc aagtattatt ggttttcagc
    23341 cagctgacga tcgtggcggc gtttaaacat ccagccaatc aacagcacaa caatccacgc
    23401 gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa ccaatcaacc cgataataaa
    23461 gagcaggaaa atcagcccgc cgatggtcgt tgctacccca cccggcactt taaatttcag
    23521 cgccttaact tcttctggcg gcaaacggcg acggaaggcg atttgcgaca gcaggatcat
    23581 aatccacacc cataccgtgg cgaaggttgc cagcgaagcg atcaccagga agacgttttc
    23641 cggcatgatg tagttcagat acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt
    23701 aacccacgga ataccgcgac gcgacgtttt gctgaaaatt ttcggtgcgc tgccctgctc
    23761 tgccataccg tggagcatac ggcctacgcc aaatacgtca ctgttaattg ccgacagcga
    23821 agcggtcagc acaacaaagt taagaatgct ggcggcaaag gtaatgccca tatgctggaa
    23881 cgtcagtacg aacgggctac cggcagtgcc aacctgattc cacgggtaga tagacataat
    23941 gacgaacagc gtaccaacgt agaacaccag aatacgcatc ggcacggagt taatcgcacg
    24001 cggaatcgat ttctcaggat ctttcgcttc accggcggta atcccgataa tttcgatccc
    24061 accgtaagca aacatcacca tttgcaacga cattaccatg ccaagccagc cgttactgaa
    24121 gaagccgccg ttgctccaca ggttatgaat accggtcggt tgcccgccgt tgccaatccc
    24181 ccagatgatg atgccgaaac cggcgacaat catgatgatg atggtggcga ctttaaagaa
    24241 cgagaaccag aattccagct caccgaagac cttcacgctc atcaggttta cggcgcaaat
    24301 gatcagcacc acgctcagta cccaaatcca gtgcggcacc gtcgggaaca agacacccat
    24361 atagatacca aaagcggtca catcggcgat ggcgacaata aggatttcaa aacagtaggt
    24421 ccatccggta atgtaacctg ccagcgggcc aaggttttcc tgcgcataac gcgagaaaga
    24481 gctggcggcc gggttatgta ccgacatttc ccccagcgca cgcataatga tatacgccgc
    24541 gataccaccg ataatatagg ccaacaacac gctcggaccg gccattttga tggcgtctgc
    24601 cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt gccataaagc gtatgtggcg
    24661 ggtacttagc ccacgcttta gcttattctt actttccatc aattcctaac ccatcaacac
    24721 aataaaaaac cacggggcat cgcagccccg tggttaaaac aaatgttcag tgatttagtg
    24781 agcgctggag gtcacctgac gacctgccgc gcgatcccag ataatggcca gaaccaccat
    24841 caccactgtt ggcattaacc acgccagacc ttgttcggcc agcggtaaac gctgcgccca
    24901 ggacggtaag atatcgctga atgcagatgc tttgatcccg tcgagaatac caaaaagcag
    24961 gctgataaac atcggcggag caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa
    25021 acttaataca accagtgcga tacacggcgg ataaatagcg gtcagcaccg gtacggagat
    25081 ctggatcagc tggcttaagc cgaggttaga caccaccatc gagaagccgc cgaggataaa
    25141 caccagcgta cgataagaga gcggtacgta ctgggcaaag aattctgcac aagcacaggt
    25201 caggccaacc gccgtgacca gacaggcgat gaagattaac gccgccagca ggaagctacc
    25261 gccgccgcca aaggtgtgct gaacgtaagc atgcagaata gcagcgccgt ttgcagactg
    25321 atcgaccagc gacgcgctgt ctgaacccag acagaacagc gccaggtaca gcaaagtcag
    25381 accaacaccc gccatcaggc cagcccagac ggtataacgg gtcagcagac gcgcttcggt
    25441 aacgccacga gaacgcgccg cgttaacaat aacgatacca aacaccattg cgcccagcgt
    25501 atccatggtc agatagccgt taacgaagcc gttagaaaat gcagcgtttt gataagcctc
    25561 agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg acaacagaca ggatgaccag
    25621 cgcgataatt ttcagcggcg caaggaagtt gcccacggta tccagcagct tgcccggata
    25681 gagcgaaacc agaataacga tagcgaaata gaccaggctg tagataaaca gcggcagcgc
    25741 ggaatcaccc gtcagcggcg caatccccac ttcaaaggaa acggtagctg tacgcggcgt
    25801 agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc agcagtacgc cagcgacttt
    25861 accgattggc gtgctgaggc tgtcaacacc gccgccaact tttgccagcg ccactaccgt
    25921 taataccggc aggccaacgg cagtaatgag gaagccgaat gccgcagtcc agacgtgttc
    25981 gcctgcctgt aagccgacca ttggagggaa aataatgtta cctgcgccga cgaacaacgc
    26041 aaatgtcata aagcccagag cgatgatatc gcgcgatctt aattgatggg tcataaatca
    26101 tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt gccaatccct ggggatgata
    26161 cagcggtata tttaaacaat tttagcggga aatactccca accaggcgcg gcgaagaata
    26221 gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc acctgaagcg caatttaaac
    26281 gcttataacg ttttaaagca aggagggatc taaaaaccag aggtatatag cgataagaaa
    26341 tcaccaacca gtataaaaca ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa
    26401 aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga ctgcaaagaa aataagccag
    26461 catcggctgg cttatggcaa taaaagatga caaaggcgga ttaatcgctg tttttggcaa
    26521 ttaaacgttc cgggataaca aaactgaaac gtgttccttt tcctactgta ctctcaatat
    26581 tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat cgctaaccct aatccgctac
    26641 cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa
    26701 tatgctccgg tgcaatgccc ggtccgttat cttcaacgct aaattcggca ccgtgcggca
    26761 ctcgctgcca gcgtacggtg atatgcgtgc cttccggcgt atgattcacg gcgttataga
    26821 ccaggttcga aatcgcactg cgtagctgat cttcgttgcc agacaccttg aggccgttat
    26881 ctatctcaaa ggtaaatgtc tgttttttct gactcagagt ctgagcctcg cgctcaacaa
    26941 cgcgcagcat catcggcaca tcaacctttt cattgagcaa ttgcgtcggc gcggcttcaa
    27001 ttttcgacag cgttagcaac tgcttcacca gcccttccat ccgctgggtc tgctcgcgca
    27061 tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca
    27121 ggtaaccctg taacacggtc aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt
    27181 tacgccgcgc cccttccagt tgatgcattt gcgtgacatc acgcgccacc atcagcaact
    27241 gtttgtgggt ataaggcatg acgcgaattt ccagatgccg cccggtgttg agcaccagat
    27301 tgagcgggcg agaaaaatca cgcgttttca gatattgcgt aaactccggg taacgcagta
    27361 ggttaaggat gttctgcccg ttatcttccg gccagcgcaa accaagaatt tgttgcgcca
    27421 gaccgttaca ccagaaaata ccgccctctt ccgtggtcag caccaccgcg tcgggcagcg
    27481 actccgcgcc gctacgaaag cgtttaatca gattgcccag ttcacggcgg cgttttttat
    27541 ttcgcagctg catctggtgt aagccgtata gtagcggttc ccagctacca cgccccggtg
    27601 gcggggtcat actgcgatcc acccacagcc accatgaaag gcgcaataaa ttccagaaat
    27661 gccagataag cagtcctgtt accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg
    27721 cacccaggat gaaagccggg aggcagcaaa gtagcagctc cagcaccagc cttttccacg
    27781 acagccgttc cagcacgtaa gatactccag ttaagaaatc ataagccctg ctctgcgtcc
    27841 gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca
    27901 ccatgcggtc atgcccgccg ggctccagtg ctttacgcag gcgacgaatg tggacatcga
    27961 ccgtgcggtc ttccacatac acgttagttc cccagacgtg gtttaacagc tgctcgcggc
    28021 tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag tttaaattct gtcggcccca
    28081 tctccagcgg ctcttcgccc gccatcactc ggtgagatgt cgggttgaga cttaatccct
    28141 gcatcttaat cacctcttcc accgccattg gcgaaatacg gcgcattacc gctttgattc
    28201 gcgccaccag ctccttcggc gaaaatggct tggtgatata gtcatccgcg ccggtttcaa
    28261 ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa catcaccact ggaatatccc
    28321 gggtcatcga ctcgcgcttg aggtgtttga tgaactggat accggagccg ccaggtaaca
    28381 tccagtcaag gagaattaaa tccggccagg gttcattcag ttgattcaca gcactgtcat
    28441 aatcttccgc ttcgaccggc tgaaagccat tttgttcgag cacgaagcag accatttcgc
    28501 gaattggagc ttcatcttct acgaccagaa tacgtctcgc catgatttgc cctgttgtaa
    28561 taaataggtt gcgatcatta atgcgacgtc attatgcgtc agatttatga cagatttatg
    28621 aaaagctcgt cgcacatatc ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg
    28681 gcacatctgt ttgggtataa tcgcgaccat gctttttcgc cagggaaccg ttatgcgcat
    28741 ccttcacacc tcagactggc atctcggcca gaacttctac agtaaaagcc gcgaagctga
    28801 acatcaggct tttcttgact ggctgctgga gacagcacaa acccatcagg tggatgcgat
    28861 tattgttgct ggtgatgttt tcgataccgg ctcgccgccc agttacgccc gcacgttata
    28921 caaccgtttt gttgtcaatt tacagcaaac tggctgtcat ctggtggtac tggcaggaaa
    28981 ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc atggcgttcc tcaatacaac
    29041 cgtggtcgcc agcgccggac atgcgccgca aatcttgcct cgtcgcgacg ggacgccagg
    29101 cgcagtgctg tgccccattc cgtttttacg cccgcgtgac attatttcca gccaggcggg
    29161 gcttaacggt attgaaaaac agcagcattt actggcagcg attaccgatt attaccaaca
    29221 acactatgcc gatgcctgca aactgcgcgg cgatcagcct ctgcccatca tcgccacggg
    29281 acatttaacg accgtgggtt ccagtaaaag tgacgccgtg cgtgacattt atattggcac
    29341 gctggacgcg tttccggcac aaaactttcc accagccgac tacatcgcgc tcgggcatat
    29401 tcaccgcgca cagattattg gcggcatgga acatgttcgc tattgcggtt cccccattcc
    29461 actgagtttt gatgaatgcg gtaagagtaa atatgtccat ctggtgacat tttcaaacgg
    29521 caaattagag agcgtggaaa acctgaacgt accggtaact caacccatgg cggtgctgaa
    29581 aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg cgcgatgtat cgcaggagcc
    29641 acctgtctgg ctggatatcg aaatcactac tgatgagtat ctgcatgata ttcagcgcaa
    29701 aatccaggca ttaaccgaat cattgcctgt cgaagtattg ctggtacgtc ggagtcgtga
    29761 acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga
    29821 agaggtgttc aatcgccgtc tggcactgga agacctggat gaatcgcagc agcaacgtct
    29881 gcagcatctt ttcaccacga cgttgcgtac cctcgccgga gaacacgaag catgaaaatt
    29941 ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg aatggaagat tgatttcacc
    30001 cgcgagccgt tcgccagcaa cgggctgttt gccattaccg gcccaaccgg tgcggggaaa
    30061 accaccctgc tggacgccat ttgtctggcg ctgtatcacg aaactccgcg tctctctaac
    30121 gtttcacaat cgcaaaatga tctcatgacc cgcgataccg ctgaatgtct ggcggaggtg
    30181 gagtttgaag tgaaaggtga agcgtatcgc gcgttctgga gccagaatcg ggcgcgtaac
    30241 cagccggacg gtaatttgca ggtgcctcgc gtagagctgg cgcgctgcgc cgacggcaaa
    30301 attctcgccg acaaagtgaa agataagctg gaactgacag cgacgttaac cgggctggat
    30361 tacgggcgct tcacccgttc gatgctgctt tcgcaggggc aatttgctgc cttcctgaat
    30421 gccaaaccca aagaacgcgc ggaattgctc gaggagttaa ccggcactga aatctacggg
    30481 caaatctcgg cgatggtttt tgagcagcac aaatcggccc gcactgagct ggagaagctg
    30541 caagcgcagg ccagcggcgt cacgttgctc acgccggaac aagtgcaatc gctgacagcg
    30601 agtttgcagg tacttactga cgaagaaaaa cagttaatta ccgcgcagca gcaagaacaa
    30661 caatcgctaa actggttaac gcgtcaggac gaattgcagc aagaagccag ccgccgtcaa
    30721 caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc aacctcaact ggcggcgctt
    30781 agtctggcac aaccggcacg aaatcttcgt ccacactggg aacgcatcgc agaacacagc
    30841 gcggcgctgg cgcatattcg ccagcagatt gaagaagtaa atactcgctt acagagcaca
    30901 atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc agtcagcaga attacagcag
    30961 cagcaacaaa gcctgaatac ctggttacag gaacacgacc gcttccgtca gtggaacaac
    31021 gaactggcgg gttggcgtgc gcagttctcc caacaaacca gcgatcgcga gcatctgcgg
    31081 caatggcagc aacagttaac ccatgctgag caaaaactta atgcgcttgc ggcgatcacg
    31141 ttgacgttaa ccgccgatga agttgctacc gccctggcgc aacatgccga gcaacgccca
    31201 ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc cccaacaaaa acgtctggcg
    31261 cagttaatgg tcactatcca gaatgtcact ctggaacaga cgcaacgtaa tgtcgcactt
    31321 aacgaaatgc gccagcgtta taaagaaaag acgcagcaac ttgccgatgt gaaaaccatt
    31381 tgcgagcagg aagcgcgcat caaaacgctg gaagcccagc gtgcacagtt acaggcgggt
    31441 cagccttgtc cactttgtgg ttccaccagc cacccggcgg tcgaggcgta tcaggcgctg
    31501 gagcctggtg ttaatcagtc tcgattactg gcgctggaaa acgaagttaa aaagctcggc
    31561 gaagaaggtg cggcgctgcg cgggcaactg gatgcattaa cgaagcagct tcagcgcgat
    31621 gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac ttactcaaca atggcaagcc
    31681 gtcacggcca gcctcaatat caccttgcag ccacaggacg atattcaacc gtggctggat
    31741 gcacaagatg agcacgaacg ccagctgcgg ttactcagcc aacgacatga attacaaggg
    31801 cagattgccg cgcataatca gcaaattatc cagtatcaac agcaaattga acaacgtcag
    31861 cagcagctct taacggcatt ggcgggttat gcactgacat tgccacagga agatgaagaa
    31921 gagagctggt tggcgacacg tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa
    31981 ttaaccgcgc tgcaaaaccg tattcagcag ctgacaccga ttctggaaac gttgccgcaa
    32041 agtgatgatc tcccgcacag cgaagaaact gtggcgctgg ataactggcg gcaggttcat
    32101 gaacaatgtc tcgcattaca cagccagcag cagacgttac agcaacagga tgttctggcg
    32161 gcgcaaagtc tgcaaaaagc ccaagcgcag tttgacaccg cgctacaggc cagcgtcttt
    32221 gacgatcagc aggcgttcct tgcggcgcta atggatgaac aaacactaac gcagctggaa
    32281 cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca
    32341 gcagaaacgc tggcacagca tcaacaacac cgacctgacg ggttggctct cactgtgacg
    32401 gtggagcaga ttcagcaaga gttagcgcaa actcaccaaa agttgcgtga aaacaccacg
    32461 agtcaaggcg agattcgcca gcagctgaag caggatgcag ataaccgtca gcaacaacaa
    32521 accttaatgc agcaaattgc tcaaatgacg cagcaggttg aggactgggg atatctgaat
    32581 tcgctaatag gttccaaaga gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg
    32641 gataatttag tccatctcgc taatcagcaa cttacccggc tgcacgtgcg ctatctgtta
    32701 cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg atacctggca ggcagatgcg
    32761 gtacgcgata cccgtaccct ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg
    32821 ctggcgcttt cggatctggt cagccataaa acacgtattg actcgctgtt ccttgatgaa
    32881 ggttttggca cgctggatag cgaaacgctg gataccgccc ttgatgcgct ggatgccctg
    32941 aacgccagtg gcaaaaccat cggtgtgatt agccacgtag aagcgatgaa agagcgtatt
    33001 ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct acagcaaact ggaaagtacg
    33061 tttgcagtga aataactatt cagcaggata atgaatacag aggggtgaat tatctcttgg
    33121 ccttgctggt cgttatcctg caagctatca ctttattggc tacggtgatt ggtagccgtt
    33181 ctggtggttg tgatggtggt atgaaaaaag tcattttatc tttggctctg ggcacgtttg
    33241 gtttggggat ggccgaattt ggcattatga gcgtgctcac ggagctggcg cataacgtag
    33301 gaatttcgat tcctgctgcc gggcatatga tctcgtatta tgcactggtg gtggtggtcg
    33361 gtgcgccaat catcgcactc ttttccagcc gctactcact caaacatatc ttgttgtttc
    33421 tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc
    33481 tcgccattgg tcggctggta tccggctttc cgcatggcgc attttttggc gtcggagcga
    33541 tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc cgccgtggcg gggatggttt
    33601 ccgggatgac agtcgccaat ttgctgggca ttccgctggg aacgtattta agtcaggaat
    33661 gctggcgtta caccttttta ttgatcgctg tttttaatat tgcggtgatg gcatcggtct
    33721 atttttgggt gccagatatt cgcgacgagg cgaaaggaaa tctgcgcgaa caatttcact
    33781 ttttgcgcag cccggccccg tggttaattt tcgccgccac gatgtttggc aacgcaggtg
    33841 tgtttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt ttttcggaaa
    33901 cggcgatgac ctttattatg atgttagttg ggctagggat ggtgctggga aatatgctaa
    33961 gtggcaggat ttcaggacgt tattcaccac tgcgcattgc agcagtgact gactttatca
    34021 ttgtactggc actgctgatg ctctttttct gcggcggcat gaaaacaacg tcgcttattt
    34081 ttgcttttat ttgttgcgcg ggattatttg ccctttcagc acctctgcaa atattgttac
    34141 tgcaaaatgc caaaggagga gagttattag gtgccgcagg tgggcaaata gcgtttaacc
    34201 tcggtagcgc cgtcggcgca tattgcggag gtatgatgct gacgctgggg ctggcatata
    34261 attacgtggc gctgcctgcc gccctgcttt cgtttgctgc gatgtcatcg ttgctgctgt
    34321 atggtcgcta taagcgccag caagcggcgg atagtccggt gctggcgaaa ccactggggt
    34381 agattactgt cacggtgcgc gttgcttatt gccggatgcg gcgtgaacgc cttatccgcc
    34441 ctacgcggtt ctggcacatt ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca
    34501 tgatgcgcca attgcctacg ttttttactc ttgtggccat aaccacgcag cgccgcgtac
    34561 gccgctggaa tcaccgtgct tcgccttact caccggcgtt tcacattcgc cgccgaagac
    34621 aaattgttta atcaactgcc caaccgtttg atataaacgg tctacattgc tcatcccgcc
    34681 ccccaggaca atcacatccg gatcgagaat attcacgaca tgtgccagcg attttgccag
    34741 ccgcagctcg tagcgacgca atgccagttc cgctaccgga tcgctttctt caaccaggcg
    34801 gataatttca ctgcctttca gcgcatgtcc gctcaaacga cgataatccg tcgcgaatcc
    34861 cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg caataacaag ggacttcctc
    34921 gcgataacgc agttcgtctt cgtccatcca cggtagcgga ttgtgtcccc actcacctgc
    34981 cgtgccattg ccgccgatat gcgtccgccc attgaatgcc acgcccgcgc cgcatcccgt
    35041 gccgataatc acggcaaata ccgtctgcgc tcccgctgcc gcgccatcta ctgcttctga
    35101 aaccgccaga cagttggcat catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa
    35161 gtctttatcg aatggctgac cgttgagcca ggttgaattg acattcttca ccacaccggt
    35221 gtagggcgaa atcgaacctg gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg
    35281 ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg
    35341 cgtgggcaga cgatggcggt acaactgctc ccctgcatcg cccagtgcaa tcacttcagt
    35401 tttggtgccg cctaaatcga tacctatacg cactgtactc tccttatttt tttcaatatc
    35461 aatagcgtag agacggacaa ccggattggc aatgcaaggc cgccgacaat tcgttatcat
    35521 gcccgctaaa tttaacgaca aggccgtgga aattatcatg ctgtggttca aaaatttaat
    35581 ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa gagatggaaa aacagctagc
    35641 ctcgatggca tttaccccat gcggcagcca ggacatggcg aagatgggct gggttcctcc
    35701 gatgggatcg cacagcgatg cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc
    35761 gcgcaaagaa gaaaaaatcc tcccgtctcc ggtgattaaa caggcgctgg aagcgaaaat
    35821 cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa
    35881 agacgaagtg ctgcactctc tgctgccgcg tgctttcagc cgttttagcc agacaatgat
    35941 gtggctcgac acggttaacg gtttgattat ggtggactgc gccagtgcca aaaaagcgga
    36001 agatacgctg gcattactgc gtaaaagcct ggggtcgtta ccggttgtac cattgagcat
    36061 ggaaaacccg attgaactga cgctgaccga atgggttcgc tccggtagtg cggcacaggg
    36121 cttccagctg cttgatgaag ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg
    36181 cgcgaagaaa caagatctga ccagcgaaga gatcaccaat cacattgaag ccggaaaagt
    36241 ggtgactaaa ctggcgctcg actggcagca gcgcattcag tttgtgatgt gcgacgatgg
    36301 ttcgctcaag cgtcttaagt tctgcgacga gctgcgcgat caaaacgaag atatcgaccg
    36361 tgaagatttc gcccagcgtt ttgatgccga tttcatcctg atgactggtg aactggcagc
    36421 gttaattcaa aacctgattg aaggattagg tggcgaagca caacgttaat tgctgatttt
    36481 cctttaatgc cggatgcgac gcctgccgcg tcttatccgg cgtacgaagc cacaccaggc
    36541 atataattat tcgctacggc gagcaataat ttttagcgca gcaatattat gcgttttacg
    36601 ccgtaactga tctaatcgcc tcatccctat tcgcctgtca gcagcacgca gaatattcac
    36661 taacccccat ttatcgctgg taaattgttt tgttaacagc tccgcttttg gggtatcaat
    36721 atattcacgc aataaatcag aaattgaaca aatatccgtc aggtaaggat accagcaagt
    36781 ttcaccgtga aaattacgca ctccagcgtt acctgcaata aagtctttcg ggtaatccca
    36841 gatcacgttt ttatccaacg tgatccaata acgcggtaaa tcggtacttc caccattttg
    36901 gctgcgcatg ggataacggg tacagtgaat ttgcaggttg atcgttggcg tcagtaagtc
    36961 atagatctcc cgctgtaact tgctccatgg acgttgtgtc attgtttttc ctcaagccgg
    37021 tgacgcgctt cactgcgaat tttccgcgtt gccccggttt tcactaaatc acgtaactgc
    37081 gaaagatcgg gttgttcgac aaataagagt aatgatggat cctcttttaa ccacgccgtt
    37141 ttcacatcgg cagccagctg tggattatta atagccagcg accgatcctg aggctcagtc
    37201 aatgtcgtta acaacgacgc cggggtatgt gcgttgcgta atgccgtttg gcgcaaatga
    37261 tcattgcgac tttgcagcaa tgcacgccac cattcatcat ctgatttatc ttttatcgta
    37321 gccagttccg caaaaaatac ctggagatct tcactgatat ttatatcaac cttgatgaat
    37381 tatgaatata tcggaaattg aaaaaatcgc agcaaaggaa atattattat gggttaaaga
    37441 gaggatattt cagaaaaatg ccctcccgtt caaggggaag gcgaggaatt acagatagcg
    37501 gcacagataa gaggtaggtt cggcaacttg aagatgaaac tcactgtgac cgggaacatt
    37561 aaacaccgaa ccggcttcat acacttgcca gtcggtcgcg tcaggcagta acacattcag
    37621 cgcgccactg attaccgtca tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc
    37681 aaccataaca cccacgctgg cgcgaccagt gctgctgctg gaaaagccga tcgatttcac
    37741 tttgccggaa aagtactcat tactttgaag cataaactgg ccctgtctgg tagttgaaca
    37801 tctacttcac tataggggcc agaggcgctg gctgtcacgc aaaattacac cagtaattcc
    37861 gcagccagcc gtgcaatcaa tacgttagag agcaagacag gaacatcgag ctgtttttgc
    37921 agtaaatcgc gatgacgttg gtgaaatcct aaacaatcca gcatgatgac atcagcccct
    37981 tttgccagta attctttccc agcatcaatg attttttgtt ctgaatcatg aatggggtta
    38041 cccaatgaaa ataccggcga tttctgcaaa atttgccatt tttgcgcctg aacgggcagc
    38101 atctcctcaa ccggaacgat aacccccacc tgatgatctt caacaataga ggaaaccagt
    38161 ggaggcaata ttcgcgacgg ctcaagaaag atcgtattac gcgcagtcat actactaatg
    38221 tttgctgtac tcattaataa aatgacgtca taaccctgat tatcgagcac ttcaaccaca
    38281 ccttgcagat ctcgctccac tttacgacgc gaaacatggg ccagctggtt gtcatttaat
    38341 aatgtgagaa tggtgtcttc gcctgcttct ggcgcgtact ccgccatcac ttcttcacga
    38401 cttaacttcc ccagcaggct atgatgggaa atattatctt cgtcaatgta ttccgtcagg
    38461 agcggcaaaa cctcctgcat aggtacaatg ccgatggtca ggatcgccaa cgacgcactc
    38521 attgttcatc caccttactt ttctttcgtc gttaattacc gggcaagtgt gaagcaccac
    38581 tgctgacact tacttgtgct acaaatgaca aaaagcgtag cagcagacgt gcacggcata
    38641 atgtaaagat tccaaatgat tccagtaatg gaatgttatt gtttaatatt ctaattatta
    38701 gaaaaacata aattatgaaa aatgtgacgc agatcccttg tagtggcact cagaatccct
    38761 tccgagcagt ctgatgcggt cactctttgt cagggtcctc atagcgtttt ttcgcatcca
    38821 tcgcttcctg agccgtcgga tgttcactga tcaacgagtc tggtttagga tgatcggctc
    38881 tgagttggta ccaggttacc gtctgtcctg gctttccttt ttcaatcgtc actatatatg
    38941 cttcacgagg ataaggcggt ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt
    39001 taagtatagg cgctctaaaa tcaacaattg atcgtctgtg ccagggcgct acgaatttca
    39061 gaaatcacct ggctgggttc gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat
    39121 agcgcatcgc gttcttccag cacttcctga acttcttcgc tcagcggttt tcccgttaag
    39181 gttggccgta aatcttcttc cggtgcagct tgcagtcggt taaccaggac tgatactggc
    39241 gcacacaaat aaaccacgat cccgttattt tgcatgaagt gacgattaaa ttccgtcaga
    39301 ataatgccgc cgcctgtagc gataacggtg gatgccgcag ttaccgcttc cagcgccgcc
    39361 gtttctctgg cgcgaaatcc cgcccactct tccctttcga cgatctccgc gaccgtcata
    39421 ttgagctgtg attgcaacca ctgatcggta tcgacaaacc gacggttaag cgaatcggca
    39481 agggccattc cgaccgttgt tttaccacag ccccgaggcc cgatcagaaa aagaggttgt
    39541 gtcatcgtgg gttttcccca ataggtcgca atgcggcgaa agccggtgtc atgagaatag
    39601 cgatcatacc atcaaactag tacaatttcg attgtaaaga aaaaattcca cttaaagtaa
    39661 aaatctcaat acatccctta ctataccaat aaatattcaa gaatgaagtg taaataataa
    39721 attacattta gccacgacta cgttgcactt ccagccacca cttctccagc tccgcggcaa
    39781 aggcctggcg gtcacgttgt gaaaggctat ctggtccgcc ggtctggatc ccactggcac
    39841 gtaaggtatc cataaaatcg cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac
    39901 gttcgccgcg cgaattaagc gccgctgcgc ctttctcgat ggcttcagca gccaaaggta
    39961 tatctgcggt gatcaccaaa tcgcccgctt cacactgccg gacaatttcg ttatcggcaa
    40021 catcgaaacc tgccgcgacg cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac
    40081 tctggtttgc taccagtacc agcggcatct gcatacgttc cgccgcgcga tacaaaatct
    40141 ctttaattac attgggacac gcgtcggcat ccacccaaat tgtcataaag tcatcctttg
    40201 ttaggtaatc ctctattgtg tcgcgctttt gccttccggc atagttctgt ttatgcttct
    40261 gccagcgatt atcaaaacaa tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc
    40321 ggttttattg gctgcggcaa tatgggaaaa gccattctcg gcggtctgat tgccagcggt
    40381 caggtgcttc cagggcaaat ctgggtatac accccctccc cggataaagt cgccgccctg
    40441 catgaccagt tcggcatcaa cgccgcagaa tcggcgcaag aagtggcgca aatcgccgac
    40501 atcatttttg ctgccgttaa acctggcatc atgattaaag tgcttagcga aatcacctcc
    40561 agcctgaata aagactctct ggtcgtttct attgctgcag gtgtcacgct cgaccagctt
    40621 gcccgcgcgc tgggccatga ccggaaaatt atccgcgcca tgccgaacac tcccgcgctg
    40681 gttaatgccg ggatgacctc cgtaacgcca aacgcgctgg taaccccaga agataccgct
    40741 gatgtgctga atattttccg ctgctttggc gaagcggaag taattgctga gccgatgatc
    40801 cacccggtgg tcggtgtgag cggttcttcg ccagcctacg tatttatgtt tatcgaagcg
    40861 atggccgacg ccgccgtgct gggcgggatg ccacgcgccc aggcgtataa atttgccgct
    40921 caggcggtaa tgggttccgc aaaaatggtg ctggaaacgg gagaacatcc gggggcactg
    40981 aaagatatgg tctgctcacc gggaggcacc accattgaag cggtacgcgt actggaagag
    41041 aaaggcttcc gtgccgcagt gatcgaagcg atgacgaagt gtatggaaaa atcagaaaaa
    41101 ctcagcaaat cctgatgact ttcgccggac gtcaggccgc cacttcggtg cggttacgtc
    41161 cggctttctt tgctttgtaa agcgccaaat ctgccgattt caaccactca cgatagtgac
    41221 tcatttgtgg gttcagcggc gcaaccccca cactaatccg taaagttacc tgtggcgtat
    41281 tcggcaagcg taacgtgttt agcccttcac gcacccgtaa catggcggta atggcgctct
    41341 cagctggcgt accggacatg attactgcaa actcatcgcc gccaaaccga ccaatcacat
    41401 cgctaccgcg cagggtaatt tgtaactgtc gggtaagcgc cacaatcgct tcatcgccca
    41461 catcatgacc ccaggtatcg ttgatgctct tgaaatggtc gatatcgata atcagtaacg
    41521 ttgcatcgcg attatgccgc cgacagttat caaattcatt gcgtaacata gtttcccaat
    41581 gacgtcggtt atacacgccc gtcatgccgt cgcgagtact catgacctgc aacctgcgtt
    41641 tatgttccgc cagtttggtt gccgtctggt agctgaccca gccaaacatc agaggataaa
    41701 cgacaataat gggaagggag agccaccatt ccagcggagc actattgaac gacacggtaa
    41761 tgcccgtaag ctcgagggtg acaaggcagg aaaccaccat caacaccaga cccgcgacaa
    41821 acagacgggg gccgcctgcc cccatcaaat tcagacacag aatcatcaac atagcggtgg
    41881 aaggcagcac gtttacgccc attacgccta cccacattcc cgctaatact gcatcggttt
    41941 ttaagttgta aatttcccgg ctaagcggat cgacggccct gctcgctatc tgccaggcta
    42001 aatgcggcca gacgaacgcc cagccgacca acaccagcca ccaccagccc ggcggcgggt
    42061 gcgaaaccag cgttgaagca atcggtaaga acatgccagc caggccaacc gcacggggta
    42121 gtctgacgcg acgggcgaag cgcagcccgg accgctgatg ttcgttttga ggagttaaag
    42181 gaggttcctc cccatgcgcc gccgcttttt tgaaaaagtt ttcatcattc attatttttg
    42241 ggaacattca gaaacaattt tcccaaatta tagagacggg gctaaaggca gaagtatgaa
    42301 atttcgggtg agccactttc gtagctcacc cgttagcact
//
LOCUS       DAZPDE010000018        40890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPDE010000018 DAZPDE010000000
VERSION     DAZPDE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.018"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHU0J_000868"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACHU0J_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1215676.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACHU0J_000869"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACHU0J_000869"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1215677.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHU0J_000870"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACHU0J_000870"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1215678.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHU0J_000871"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACHU0J_000871"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1215679.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACHU0J_000872"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACHU0J_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1215680.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACHU0J_000873"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACHU0J_000873"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1215681.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACHU0J_000874"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACHU0J_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1215682.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHU0J_000875"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACHU0J_000875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1215683.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACHU0J_000876"
     CDS             complement(8942..9223)
                     /locus_tag="ACHU0J_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215684.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACHU0J_000877"
     CDS             complement(9237..10898)
                     /locus_tag="ACHU0J_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1215685.1"
                     /translation="MTAWNKDAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACHU0J_000878"
     CDS             complement(10882..11208)
                     /locus_tag="ACHU0J_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1215686.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACHU0J_000879"
     CDS             complement(11264..11446)
                     /locus_tag="ACHU0J_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215687.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACHU0J_000880"
     CDS             complement(11430..11870)
                     /locus_tag="ACHU0J_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1215688.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACHU0J_000881"
     CDS             complement(11870..12166)
                     /locus_tag="ACHU0J_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR1215689.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACHU0J_000882"
     CDS             complement(12163..12501)
                     /locus_tag="ACHU0J_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR1215690.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACHU0J_000883"
     CDS             complement(12498..13709)
                     /locus_tag="ACHU0J_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1215691.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACHU0J_000884"
     CDS             complement(13711..14283)
                     /locus_tag="ACHU0J_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR1215692.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACHU0J_000885"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACHU0J_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="ACHU0J_000886"
     CDS             complement(15781..16005)
                     /locus_tag="ACHU0J_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215693.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16046..16159)
                     /locus_tag="ACHU0J_000887"
     CDS             complement(16046..16159)
                     /locus_tag="ACHU0J_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1215694.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16234..16308)
                     /locus_tag="ACHU0J_000888"
                     /pseudo
     CDS             complement(16234..16308)
                     /locus_tag="ACHU0J_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16318..16623)
                     /locus_tag="ACHU0J_000889"
     CDS             complement(16318..16623)
                     /locus_tag="ACHU0J_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215695.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="ACHU0J_000890"
     CDS             complement(16620..16898)
                     /locus_tag="ACHU0J_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215696.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17178..18926)
                     /locus_tag="ACHU0J_000891"
     CDS             complement(17178..18926)
                     /locus_tag="ACHU0J_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR1215697.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18923..19222)
                     /locus_tag="ACHU0J_000892"
     CDS             complement(18923..19222)
                     /locus_tag="ACHU0J_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1215698.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19228..19461)
                     /locus_tag="ACHU0J_000893"
     CDS             complement(19228..19461)
                     /locus_tag="ACHU0J_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215699.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19463..19683)
                     /locus_tag="ACHU0J_000894"
                     /pseudo
     CDS             complement(19463..19683)
                     /locus_tag="ACHU0J_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19676..19897)
                     /locus_tag="ACHU0J_000895"
     CDS             complement(19676..19897)
                     /locus_tag="ACHU0J_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215700.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19887..20087)
                     /locus_tag="ACHU0J_000896"
     CDS             complement(19887..20087)
                     /locus_tag="ACHU0J_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1215701.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20217..20396
                     /locus_tag="ACHU0J_000897"
     CDS             20217..20396
                     /locus_tag="ACHU0J_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215702.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20521..20850
                     /locus_tag="ACHU0J_000898"
     CDS             20521..20850
                     /locus_tag="ACHU0J_000898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215703.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20900..21088)
                     /locus_tag="ACHU0J_000899"
     CDS             complement(20900..21088)
                     /locus_tag="ACHU0J_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1215704.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21463..22692)
                     /locus_tag="ACHU0J_000900"
     CDS             complement(21463..22692)
                     /locus_tag="ACHU0J_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1215705.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22941..24062
                     /gene="roxA"
                     /locus_tag="ACHU0J_000901"
                     /gene_synonym="ycfD"
     CDS             22941..24062
                     /gene="roxA"
                     /locus_tag="ACHU0J_000901"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1215706.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHU0J_000902"
     CDS             complement(24111..25337)
                     /gene="pepT"
                     /locus_tag="ACHU0J_000902"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1215707.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25587..26723
                     /gene="potA"
                     /locus_tag="ACHU0J_000903"
     CDS             25587..26723
                     /gene="potA"
                     /locus_tag="ACHU0J_000903"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1215708.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26707..27564
                     /gene="potB"
                     /locus_tag="ACHU0J_000904"
     CDS             26707..27564
                     /gene="potB"
                     /locus_tag="ACHU0J_000904"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1215709.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27561..28355
                     /gene="potC"
                     /locus_tag="ACHU0J_000905"
     CDS             27561..28355
                     /gene="potC"
                     /locus_tag="ACHU0J_000905"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1215710.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28352..29398
                     /gene="potD"
                     /locus_tag="ACHU0J_000906"
     CDS             28352..29398
                     /gene="potD"
                     /locus_tag="ACHU0J_000906"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1215711.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHU0J_000907"
     CDS             complement(29554..30375)
                     /gene="cobB"
                     /locus_tag="ACHU0J_000907"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1215712.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHU0J_000908"
     CDS             complement(30391..31302)
                     /gene="nagK"
                     /locus_tag="ACHU0J_000908"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1215713.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHU0J_000909"
     CDS             complement(31331..32575)
                     /gene="lolE"
                     /locus_tag="ACHU0J_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1215714.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHU0J_000910"
     CDS             complement(32575..33276)
                     /gene="lolD"
                     /locus_tag="ACHU0J_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1215715.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHU0J_000911"
     CDS             complement(33269..34468)
                     /gene="lolC"
                     /locus_tag="ACHU0J_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1215716.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34730..35803
                     /locus_tag="ACHU0J_000912"
     CDS             34730..35803
                     /locus_tag="ACHU0J_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1215717.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35931..39377
                     /gene="mfd"
                     /locus_tag="ACHU0J_000913"
     CDS             35931..39377
                     /gene="mfd"
                     /locus_tag="ACHU0J_000913"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1215718.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHU0J_000914"
     CDS             39520..40482
                     /gene="ldtC"
                     /locus_tag="ACHU0J_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR1215719.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40565..40777)
                     /locus_tag="ACHU0J_000915"
     CDS             complement(40565..40777)
                     /locus_tag="ACHU0J_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR1215720.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9621 a        10794 c        10102 g        10373 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgtc cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat tgcgttagtc
    17221 tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt cgcggctggc
    17281 ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc gtacgccata
    17341 aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga atcgccaccg
    17401 ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca aaactcaata
    17461 accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg ttccagtagt
    17521 aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat aatgacaggg
    17581 atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact aacgatattg
    17641 tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt gaatatcatc
    17701 gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg cttttcgtat
    17761 ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt tccttcgcct
    17821 gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg cgcacgtcca
    17881 ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc tgccagaagg
    17941 ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc ctcaataaac
    18001 atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat acgcatcatc
    18061 ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag cgcgttatcg
    18121 cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt ggtgatccag
    18181 ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc attggcgaac
    18241 ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa cgcttccacg
    18301 gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat caccaccatt
    18361 tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca tacgtgaacg
    18421 atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag ctgcgcgcgc
    18481 tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt aagccctgcg
    18541 gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt ctggacgtgc
    18601 attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt ctgaccaatc
    18661 tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg tttttttcca
    18721 tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag ccacacaggc
    18781 ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc gtgcgaccag
    18841 gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc gcgtggctgt
    18901 ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc atctttttta
    18961 tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg ggcgctgcct
    19021 gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca tattcgtaac
    19081 cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc atgccgccgt
    19141 actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca ctggcagtta
    19201 agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt tatatctgtc
    19261 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
    19321 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
    19381 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
    19441 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
    19501 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
    19561 tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg aaagcgcatc
    19621 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
    19681 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
    19741 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
    19801 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
    19861 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
    19921 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
    19981 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
    20041 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
    20101 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
    20161 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
    20221 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
    20281 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
    20341 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
    20401 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
    20461 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
    20521 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
    20581 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
    20641 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
    20701 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
    20761 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
    20821 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
    20881 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
    20941 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
    21001 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
    21061 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
    21121 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
    21181 gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
    21241 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
    21301 cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa tctgtacact
    21361 ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg ttgtatctgt
    21421 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    21481 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    21541 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    21601 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    21661 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    21721 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    21781 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    21841 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    21901 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    21961 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    22021 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gtttttcccc
    22081 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    22141 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    22201 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    22261 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    22321 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    22381 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    22441 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    22501 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    22561 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    22621 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    22681 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    22741 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    22801 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    22861 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    22921 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    22981 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    23041 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    23101 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    23161 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    23221 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    23281 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    23341 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    23401 gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    23461 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    23521 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    23581 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    23641 cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    23701 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    23761 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    23821 acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    23881 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    23941 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    24001 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    24061 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    24121 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    24181 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    24241 aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt
    24301 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    24361 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    24421 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    24481 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    24541 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    24601 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    24661 gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc gttgaagttt
    24721 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    24781 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    24841 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    24901 gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt aatcaacgtc
    24961 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    25021 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    25081 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    25141 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    25201 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    25261 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    25321 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    25381 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    25441 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    25501 tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca caaaccccgc
    25561 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    25621 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    25681 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    25741 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    25801 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    25861 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    25921 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    25981 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    26041 aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg ttgttgctgg
    26101 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    26161 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    26221 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    26281 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    26341 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    26401 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    26461 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    26521 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    26581 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    26641 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    26701 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    26761 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    26821 gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg tctgctcgat
    26881 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    26941 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    27001 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    27061 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    27121 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    27181 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    27241 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    27301 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    27361 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    27421 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    27481 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    27541 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    27601 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    27661 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    27721 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    27781 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    27841 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    27901 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    27961 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    28021 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    28081 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    28141 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    28201 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    28261 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    28321 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    28381 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    28441 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    28501 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    28561 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    28621 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    28681 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    28741 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
    28801 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    28861 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    28921 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    28981 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    29041 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    29101 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    29161 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    29221 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    29281 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    29341 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    29401 cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    29461 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    29521 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    29581 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    29641 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    29701 aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    29761 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    29821 cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc
    29881 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    29941 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    30001 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    30061 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    30121 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    30181 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    30241 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    30301 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    30361 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    30421 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac gagctacagg
    30481 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    30541 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    30601 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    30661 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    30721 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    30781 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    30841 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    30901 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca
    30961 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    31021 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    31081 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    31141 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    31201 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    31261 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    31321 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    31381 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    31441 ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg gagaggaact
    31501 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    31561 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    31621 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    31681 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    31741 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    31801 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    31861 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    31921 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    31981 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    32041 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    32101 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    32161 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    32221 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    32281 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    32341 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    32401 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    32461 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    32521 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    32581 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    32641 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    32701 attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    32761 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    32821 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    32881 cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct tgccaatcag
    32941 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    33001 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    33061 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    33121 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    33181 cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    33241 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    33301 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    33361 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    33421 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    33481 ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag accatcatga
    33541 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    33601 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    33661 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    33721 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactctca
    33781 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    33841 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    33901 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    33961 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    34021 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    34081 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    34141 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    34201 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    34261 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    34321 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    34381 aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg taacgcaggc
    34441 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    34501 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    34561 tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc aatcttgccg
    34621 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    34681 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    34741 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    34801 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    34861 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    34921 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    34981 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    35041 gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    35101 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    35161 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    35221 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    35281 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    35341 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    35401 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    35461 gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    35521 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    35581 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    35641 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    35701 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    35761 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    35821 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    35881 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    35941 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    36001 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    36061 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    36121 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    36181 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    36241 gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    36301 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    36361 aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca
    36421 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    36481 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    36541 aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa
    36601 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    36661 tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg cagccattgt
    36721 tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    36781 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    36841 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    36901 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    36961 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    37021 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    37081 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    37141 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    37201 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat ctggcgctga
    37261 tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    37321 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    37381 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    37441 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    37501 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    37561 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    37621 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    37681 aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa accacgccgg
    37741 atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc
    37801 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    37861 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    37921 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    37981 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    38041 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    38101 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    38161 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    38221 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    38281 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    38341 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    38401 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    38461 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    38521 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    38581 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    38641 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    38701 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    38761 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    38821 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    38881 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    38941 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    39001 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    39061 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    39121 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    39181 gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    39241 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    39301 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    39361 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    39421 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    39481 tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa aacgcgtttt
    39541 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    39601 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    39661 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    39721 ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    39781 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    39841 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    39901 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    39961 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    40021 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    40081 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    40141 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    40201 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    40261 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    40321 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    40381 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    40441 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    40501 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    40561 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    40621 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    40681 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    40741 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    40801 tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt cgcaagaaat
    40861 tacttaacga tcgataacta aatgatagat
//
LOCUS       DAZPDE010000019        38592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPDE010000019 DAZPDE010000000
VERSION     DAZPDE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.019"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            2..133
                     /locus_tag="ACHU0J_000916"
     CDS             2..133
                     /locus_tag="ACHU0J_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1215721.1"
                     /translation="MYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA"
     gene            112..216
                     /gene="speFL"
                     /locus_tag="ACHU0J_000917"
     CDS             112..216
                     /gene="speFL"
                     /locus_tag="ACHU0J_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1215722.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            527..2724
                     /gene="speF"
                     /locus_tag="ACHU0J_000918"
                     /pseudo
     CDS             527..2724
                     /gene="speF"
                     /locus_tag="ACHU0J_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2721..4040
                     /gene="potE"
                     /locus_tag="ACHU0J_000919"
     CDS             2721..4040
                     /gene="potE"
                     /locus_tag="ACHU0J_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1215723.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4096..5736)
                     /gene="pgm"
                     /locus_tag="ACHU0J_000920"
     CDS             complement(4096..5736)
                     /gene="pgm"
                     /locus_tag="ACHU0J_000920"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1215724.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5762..6307)
                     /gene="seqA"
                     /locus_tag="ACHU0J_000921"
     CDS             complement(5762..6307)
                     /gene="seqA"
                     /locus_tag="ACHU0J_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1215725.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6492..7256
                     /gene="ybfF"
                     /locus_tag="ACHU0J_000922"
     CDS             6492..7256
                     /gene="ybfF"
                     /locus_tag="ACHU0J_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1215726.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7396..7689
                     /gene="ybfE"
                     /locus_tag="ACHU0J_000923"
     CDS             7396..7689
                     /gene="ybfE"
                     /locus_tag="ACHU0J_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1215727.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7829..8359
                     /gene="fldA"
                     /locus_tag="ACHU0J_000924"
     CDS             7829..8359
                     /gene="fldA"
                     /locus_tag="ACHU0J_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1215728.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8569..8655
                     /gene="uof"
                     /locus_tag="ACHU0J_000925"
     CDS             8569..8655
                     /gene="uof"
                     /locus_tag="ACHU0J_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1215729.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8648..9094
                     /gene="fur"
                     /locus_tag="ACHU0J_000926"
     CDS             8648..9094
                     /gene="fur"
                     /locus_tag="ACHU0J_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1215730.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9178..9504)
                     /gene="chiQ"
                     /locus_tag="ACHU0J_000927"
     CDS             complement(9178..9504)
                     /gene="chiQ"
                     /locus_tag="ACHU0J_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1215731.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9541..10947)
                     /gene="chiP"
                     /locus_tag="ACHU0J_000928"
     CDS             complement(9541..10947)
                     /gene="chiP"
                     /locus_tag="ACHU0J_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR1215732.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11526..13190)
                     /gene="glnS"
                     /locus_tag="ACHU0J_000929"
     CDS             complement(11526..13190)
                     /gene="glnS"
                     /locus_tag="ACHU0J_000929"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1215733.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13393..15339)
                     /gene="nagE"
                     /locus_tag="ACHU0J_000930"
     CDS             complement(13393..15339)
                     /gene="nagE"
                     /locus_tag="ACHU0J_000930"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1215734.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            15672..16472
                     /gene="nagB"
                     /locus_tag="ACHU0J_000931"
     CDS             15672..16472
                     /gene="nagB"
                     /locus_tag="ACHU0J_000931"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1215735.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16532..17680
                     /gene="nagA"
                     /locus_tag="ACHU0J_000932"
     CDS             16532..17680
                     /gene="nagA"
                     /locus_tag="ACHU0J_000932"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1215736.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17689..18909
                     /gene="nagC"
                     /locus_tag="ACHU0J_000933"
                     /gene_synonym="nagR"
     CDS             17689..18909
                     /gene="nagC"
                     /locus_tag="ACHU0J_000933"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1215737.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18957..19709
                     /gene="nagD"
                     /locus_tag="ACHU0J_000934"
                     /pseudo
     CDS             18957..19709
                     /gene="nagD"
                     /locus_tag="ACHU0J_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(19760..19858)
                     /locus_tag="ACHU0J_000935"
                     /pseudo
     CDS             complement(19760..19858)
                     /locus_tag="ACHU0J_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20106..21770
                     /gene="asnB"
                     /locus_tag="ACHU0J_000936"
     CDS             20106..21770
                     /gene="asnB"
                     /locus_tag="ACHU0J_000936"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1215738.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22149..22225
                     /locus_tag="ACHU0J_000937"
     tRNA            22149..22225
                     /locus_tag="ACHU0J_000937"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22183..22185,aa:Met,seq:cat)
     gene            22234..22318
                     /locus_tag="ACHU0J_000938"
     tRNA            22234..22318
                     /locus_tag="ACHU0J_000938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22268..22270,aa:Leu,seq:tag)
     gene            22342..22416
                     /locus_tag="ACHU0J_000939"
     tRNA            22342..22416
                     /locus_tag="ACHU0J_000939"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22374..22376,aa:Gln,seq:ttg)
     gene            22451..22525
                     /locus_tag="ACHU0J_000940"
     tRNA            22451..22525
                     /locus_tag="ACHU0J_000940"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22483..22485,aa:Gln,seq:ttg)
     gene            22541..22617
                     /locus_tag="ACHU0J_000941"
     tRNA            22541..22617
                     /locus_tag="ACHU0J_000941"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22575..22577,aa:Met,seq:cat)
     gene            22666..22740
                     /locus_tag="ACHU0J_000942"
     tRNA            22666..22740
                     /locus_tag="ACHU0J_000942"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22698..22700,aa:Gln,seq:ctg)
     gene            22778..22852
                     /locus_tag="ACHU0J_000943"
     tRNA            22778..22852
                     /locus_tag="ACHU0J_000943"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22810..22812,aa:Gln,seq:ctg)
     gene            complement(23006..24181)
                     /gene="ubiF"
                     /locus_tag="ACHU0J_000944"
     CDS             complement(23006..24181)
                     /gene="ubiF"
                     /locus_tag="ACHU0J_000944"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1215739.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            24327..25751
                     /gene="miaB"
                     /locus_tag="ACHU0J_000945"
     CDS             24327..25751
                     /gene="miaB"
                     /locus_tag="ACHU0J_000945"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1215740.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25904..26944
                     /locus_tag="ACHU0J_000946"
     CDS             25904..26944
                     /locus_tag="ACHU0J_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1215741.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26941..27408
                     /gene="ybeY"
                     /locus_tag="ACHU0J_000947"
     CDS             26941..27408
                     /gene="ybeY"
                     /locus_tag="ACHU0J_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1215742.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27498..28376
                     /gene="corC"
                     /locus_tag="ACHU0J_000948"
     CDS             27498..28376
                     /gene="corC"
                     /locus_tag="ACHU0J_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1215743.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28401..29939
                     /gene="lnt"
                     /locus_tag="ACHU0J_000949"
     CDS             28401..29939
                     /gene="lnt"
                     /locus_tag="ACHU0J_000949"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1215744.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            30336..31244
                     /gene="gltI"
                     /locus_tag="ACHU0J_000950"
     CDS             30336..31244
                     /gene="gltI"
                     /locus_tag="ACHU0J_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1215745.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31414..32154
                     /gene="gltJ"
                     /locus_tag="ACHU0J_000951"
     CDS             31414..32154
                     /gene="gltJ"
                     /locus_tag="ACHU0J_000951"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1215746.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            32154..32828
                     /gene="gltK"
                     /locus_tag="ACHU0J_000952"
     CDS             32154..32828
                     /gene="gltK"
                     /locus_tag="ACHU0J_000952"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1215747.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32828..33553
                     /gene="gltL"
                     /locus_tag="ACHU0J_000953"
     CDS             32828..33553
                     /gene="gltL"
                     /locus_tag="ACHU0J_000953"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1215748.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33671..34606
                     /gene="rihA"
                     /locus_tag="ACHU0J_000954"
     CDS             33671..34606
                     /gene="rihA"
                     /locus_tag="ACHU0J_000954"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1215749.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            34690..36360
                     /gene="hscC"
                     /locus_tag="ACHU0J_000955"
                     /pseudo
     CDS             34690..36360
                     /gene="hscC"
                     /locus_tag="ACHU0J_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(36420..37871)
                     /gene="djlC"
                     /locus_tag="ACHU0J_000956"
     CDS             complement(36420..37871)
                     /gene="djlC"
                     /locus_tag="ACHU0J_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1215750.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEEESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37868..38575)
                     /locus_tag="ACHU0J_000957"
     CDS             complement(37868..38575)
                     /locus_tag="ACHU0J_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1215751.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9437 a         9837 c         9756 g         9562 t
ORIGIN      
        1 gatttattat ctggggtatg atcgtttttt gttgatcttc ttcacagatt atagccattt
       61 catggataga ataactctac cttcaactga cacagcaagc ggtaaaggta aatggaaaat
      121 aataaccgct taatgcctca tataaggcgg acaacccata tcatgaagtt tacccatcgt
      181 aatagttttg actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa
      241 cgtacaggtc tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat
      301 atactgctaa cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta
      361 tgacgagtat agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta
      421 ctgatttatt tatcagcggt ggagctttgc tttttttccg acgtgatcga tttctccttt
      481 gagaaattga ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa
      541 aattgccgtt agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat
      601 tgacgaaagt aattatattg acgttgccgc cattatttta tcagtcaatg atgttgaacg
      661 tggaaaactc gataaaatag acgctactgg ctatggcatt cctgttttta ttgcaacgga
      721 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
      781 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      841 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      901 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      961 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
     1021 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
     1081 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
     1141 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
     1201 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
     1261 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
     1321 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
     1381 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
     1441 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
     1501 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
     1561 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
     1621 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
     1681 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1741 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1801 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1861 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1921 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1981 agagaatcaa tactttgtcg atccttgcaa actgttgctg acaaccccag gtattgatgc
     2041 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     2101 cgaaaatggc gtagtgccgg aaaaatgcga tcttaacgcc atcctcttcc tgctgactcc
     2161 ggcagaagat atggccaaac ttcagcaact tgtgccctgc tggtacgctt cgaaaaactg
     2221 cttgagtccg acgcgccatt agcagaagtg ctgccttcca tctacaaaca gcatgaagag
     2281 cgctacgccg gttataccct gcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc
     2341 cacaacgtga aacaactgca aaaagagatg ttccgcaaag cgcacttccc acacgtcagc
     2401 atgaatccgc aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtcta
     2461 ccggatgccg aaggtcgtat cgctgccgaa ggggcgcttc cttatcctcc gggtgtgctg
     2521 tgtgttgttc cgggtgaaat ctggggtggt gctgttttgc gttacttcag cgctctggaa
     2581 gaagggatca acctgctgcc aggttttgca ccggagctgc agggtgtcta tatcgaagaa
     2641 catgatggtc gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc
     2701 ctgttgaaag gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt
     2761 taaccatact gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc
     2821 ttgccgaagt tgggacaatt tcaattatct cctggctggt gacagccgta ggctcaatgg
     2881 cactggcatg ggcattcgca aaatgcggta tgttcagccg taaatcaggt ggtatgggcg
     2941 gttatgccga gtatgctttc ggtaaatccg gtaactttat tgcgaactat acctacggcg
     3001 tctcactgct gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac
     3061 tgctcggcgc gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga
     3121 tttgtaccgt ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg
     3181 tgtggggggg cattattccg gtggtaggcc tgtgcattat cggctggttc tggtttagcc
     3241 cgacgcttta cgttgattcc tggaatccgc atcatgcacc gttcttcagt gcagtaggtt
     3301 cttccatcgc tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt gcgaatactg
     3361 atgtagtgga aaacccggaa cgtaatgtgc caatcgcggt actcggcggt acgttaggtg
     3421 cggcggtgat ttatatcgtc tccaccaacg tgattgcagg gattgtgcct aatatggagc
     3481 tggcaaattc aacggcacca tttggcctgg ccttcgcaca gatgtttaca ccagaagtgg
     3541 gtaaagtcat tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt
     3601 tcaccattgc ccaggtgttt aaatcttcat ctgatgaagg ctacttccct aaaattttct
     3661 cccgtgtaac caaagtggat gcaccggtgc agggaatgtt gaccattgtg attatccaga
     3721 gcgggttggc gctgatgacg attagcccgt cgctaaacag tcagttcaac gtgctggtta
     3781 acctggccgt ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta
     3841 ttcagaaggt cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg
     3901 ttggcgcgat gtatagcttt tatgcgctgt actcatccgg ggaagaagcc atgctgtacg
     3961 gttccatcgt gaccttcctt ggttggacac tgtatggtct ggtatcacca cgctttgaac
     4021 tgaaaaataa acacggttaa aaaagcaaag taaaaaaggg cgatcttgcg accgctcttt
     4081 ttttattaaa tgtgtttacg cgtttttcag aacttcgcta acaatctcaa ccgcttcttt
     4141 ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg cagtagatct tatatgcgtc
     4201 ttccgtgcct gacggacgcg cggcgaacca gccgttgtca gtcatcactt tcagaccgcc
     4261 aatagaagca ccgttgcccg gagcagcagt caggcgcgcg gtgatcgggt cacctgccag
     4321 ggtgctggcg ctcaccattt ccggagacag cttagacagc gccgcttttt gtgcggaagt
     4381 cgcagctgcc tgcaaacggt tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta
     4441 gtgttcctgc gggttcttac cggtgacagc ggtgatttcc gccgccagca gacacataat
     4501 gatgccgtct ttgtcggtgg accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc
     4561 gctctcttcg ccgccaaagc cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa
     4621 acctaccggt acttctacca gcttacggcc caagtcattg accacacggt cgatcatcgc
     4681 agaagaaacc agcgttttac cgacagcaac atctttgccc cactgcggac gatgctggaa
     4741 caggtagttg atcgccaccg ccaggtagtg gttcggattc atcaaacctg ccggagtgac
     4801 gataccgtga cggtcataat ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag
     4861 tgccagcaga cccgccatcg cacactcgga ggagcagtcc atacggatcg caccgtcttt
     4921 atcgaggtgc ataaagcgga aggtttgatc gacctgatcg ttaacgatgg tcaggttgag
     4981 gttgtaatac tcgccaatgc gcttccagta ttcgataccg gaaccgccca acggatcaac
     5041 gcccagcgtc aggcccgctt tctgaatcgc cgccatatca acgatatcgg ccagcccttc
     5101 cacgaacggc tgcaccagat cctgctcttt cacatagccg gatgccatcg cttcgtcgag
     5161 ggagatacgc ttcacgcctt tcaggccatc ggccatcagt gcgttggcct tgtcttccac
     5221 cactttggtg acgttagtat cagccgggcc accatttggc ggattgtact tgataccacc
     5281 atcttccggc gggttatggg acggtgtaat cacgataccg tctgccagcg ggccaccttt
     5341 tttattgtga accaggatgg cattggaaac ggcaggcgtc ggagtgaagc cattgttttc
     5401 ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg
     5461 ctcggacagg gcgtgagtat ctttacccac atagcaaggg ccagtgatgc cgtttttcgc
     5521 acgttcttca gcaattgcct gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg
     5581 cgctgcactg ccacggtgac cggaagtacc gaatttcacc gcgtgctccg cattccctgc
     5641 ttctggtttc agtacataat attgcgccgt cagttgggcg acgttaatca aatcactctg
     5701 ttgtgcaggt tggcctgcac gattgtggat tgccattgct ttgtcctttg tctgcaacgt
     5761 tttagatagt tccgcaaacc ttctcaatca attccgccgg gaattgcatc gactgcatga
     5821 tgtgttcgat catgctgcat ttacggccgg tgttggtgtt ggtgatcacc caatacggcg
     5881 tgcctggcac atgtttcggc ttggtctgat taccattttt cagcagcgtt tgttcatctg
     5941 ccgcaaagta cacgcgtgta cgaccgtgca acgattccgt tgcttcggca aacgcctggg
     6001 cgtcaagaga atatagtgta gacaacagca gcataaagcg attgaccgct cgcttttgct
     6061 ctgcgtattc gtccgaaagc agaagttcac gcattgcgcg aaccttgtct ttaatcgttt
     6121 tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg aacctctttc gtcaccggag
     6181 cagcaggctg tgatgcggcg gaaaatttca acatacgccg taaaatgtcg gatgcgctct
     6241 cgccgatatg cttagtgtgg ctggcaatat agctgtagag ttcatcatca acttcaatcg
     6301 ttttcatctt aatccagtgc agtgtcttag ctgaatacaa gtcgccaaga gtatatgaat
     6361 aaatcccggc agcggatagc gtcaagcctg gcttgcagaa aaactcggaa taaaccttat
     6421 ttgttgcagc cctgtggcac aatggtcaac ataataccct aacccgacaa cgcgaaaaaa
     6481 agaactttgc catgaaatta aatatccgcg cgcaaactgc acaaaaccag cacaataatt
     6541 ctcccatcgt tcttgtccat ggtctgtttg gtagcctcga caaccttggc gtactggctc
     6601 gcgatctggt aaacgatcac aatatcatcc aggttgatat gcgtaaccac ggtctttcac
     6661 cgagagagcc ggtaatgaat tacccggcga tggcgcagga tcttgttgat accctggatg
     6721 cactgcagat cgacaaagca acatttatcg gtcactccat gggcggtaaa gcggtaatgg
     6781 cacttactgc actggccccc gatcgcatcg ataaactggt ggcgatcgat atcgcaccgg
     6841 tcgactatca cgtacgccgt catgatgaga ttttcgcagc tatcaacgcg gtcagtgaat
     6901 cggacgcaca aactcgccag caagcagcag caataatgcg ccagcatctt aatgaagaag
     6961 gggtgattca gtttctgttg aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg
     7021 tattgtggga tcagtatccg catattgtgg gttgggagaa aatcccggca tgggatcacc
     7081 ctgccctgtt tatccctggc ggcaattctc cgtatgttag cgagcagtac cgtgatgatt
     7141 tactggcaca atttccacag gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc
     7201 atgctgaaaa accggatgcg gtattacgcg ctatccgtcg ctatctcaat gattaacgca
     7261 actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc
     7321 gcgctgatgt attatggcgc gctatccatc cgggcagagg cctggctgat tgtttccccc
     7381 gaagtcacca agatcatggc caaagaacaa acggaccgta cgacattaga tctgttcgcg
     7441 cacgagcgtc gaccgggacg accgaaaact aatccgcttt cgcgcgatga acagctgcgt
     7501 attaataaac gcaaccagct aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg
     7561 aagctgaacg cggaagctgt cgaggcgctg aacgagctgg cggaatcgcg taatatgagc
     7621 cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg cggcgctgcg tagtcagggc
     7681 atcgtttaaa tttccacttt catgtagcac agtgtgcagt cctgctcgtt tgctgattcc
     7741 gcaagactgc ctgttctgct atgattgcct ttatccgtgg gcaattttcc acccccattt
     7801 caataagttt caagaggtta tttcactcat ggctatcact ggcatctttt tcggcagcga
     7861 caccggtaat accgaaaata tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc
     7921 cgatgtccat gacattgcaa aaagcagcaa agaagatctg gaagcttacg acattctgct
     7981 gctgggcatc ccaacctggt attacggcga agcgcagtgt gactgggatg acttcttccc
     8041 gactctcgaa gagattgatt tcaatggtaa actggttgcg ctgtttggtt gtggtgacca
     8101 ggaagattac gccgaatatt tctgcgacgc attgggcacc atccgcgaca tcattgaacc
     8161 gcgcggtgca accatcgttg gtcactggcc aactgcgggc tatcatttcg aagcatcaaa
     8221 aggtctggca gatgacgacc actttgtcgg tctggctatc gacgaagacc gtcagccgga
     8281 actgaccgct gaacgtgtag aaaaatgggt taaacagatt tctgaagagt tgcatctcga
     8341 cgaaattctt aatgcctgat gtgatgcggc gtagactcat gtctacgccg tattaataga
     8401 taatgccaat caaaataatt gctacaaatt tgtaactttt gctgttgtac ctatacaatg
     8461 tcccagtgtt caagtggcct tgccgttgta aatgtaagct gtgccacgtt tttattaaca
     8521 atatttgcca gggacttgtg gttttcattt aggcgtggca attctataat gatacgcatt
     8581 atctcaagag caaattctgt cacttcttct aatgaagtga accgcttagt aacaggacag
     8641 attccgcatg actgataaca ataccgccct aaagaaagct ggcctgaaag taacgcttcc
     8701 tcgtttaaaa atcctggaag ttcttcagga gccggacaac catcacgtca gtgcggaaga
     8761 tttatacaaa cgtctgatcg atatgggtga agaaattggt ctggctacgg tatatcgcgt
     8821 actgaaccag tttgacgacg ctggtatcgt cacccgccac aattttgaag gcggtaaatc
     8881 cgtatttgaa ctgacacagc aacatcacca cgatcacctg atttgcctcg actgcggcaa
     8941 ggttatcgaa tttagtgatg attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca
     9001 tggcattcgc ctgactaacc acagtctcta tctttacggt cactgtgccg aaggcgattg
     9061 ccgcgaagat gagcacgcgc acgaaggcaa ataagccagc ctgaacgaga aaagccaacc
     9121 tgcgggttgg cttttttata caagggaaat aagaaaagcg agagttacag ctctcactta
     9181 tttgttatta ctgcgtattt cctgccagac cttatcacaa tcggctttca ccgcctgatc
     9241 atttccggtt tgcgttgcac gcagacactg ctgataatcc agtacgcgta cactttcctg
     9301 gtcggcaaac tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc
     9361 ttcaattttt tctggcgaac cttgcgcggt gttgatacag gcgctgtatg cctctttcag
     9421 acggctgtct tcctgtggtg cggttgattg cgcacaagct accagccccg atgccatcat
     9481 ggcgatgaga atcagttttt tcattgaatg cggcaggcac aaaacctgcc gcgtcgggca
     9541 tcagaagatg gtgaatggtg cgattaccat aaatttcacg tcacgctcat cctggaagat
     9601 gttaccgtaa ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg
     9661 cagtttgaac atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct
     9721 gtaggcagac tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca
     9781 ggtcgcaggt ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag
     9841 attccagttt ttcaggtcat acatcgcact gaagaagacc gctttttcgc cgttagcgtt
     9901 gaagtcggaa cggttatccc accagatatc caggcggccg ttggaggaag cgtaggttgg
     9961 ggtcatacgt tgcaggaagt atccctgctg actgtcagcc ttaatccagg tgccttcgag
    10021 gcgcaaatcc actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt
    10081 gccgtcataa agatcgttga cgctgcgatc gtcaacttta tcgcgggtac catagaactg
    10141 gtagctggtg gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaaata
    10201 ttgatcgata tacccttccg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa
    10261 gtcgtatttc gcaccgatgg agtgcagata atcaactttg gtggttttat cgttctgata
    10321 aaactcatcc atttccagat gccacggcgc tttgtattcg ttggtccaca tgtaggagaa
    10381 actcaaggca ccagcatcgc cgtaatcaaa attcgccccg gcttccgccc cctgataagt
    10441 acccggcata aagctccagt gcggcgctaa cagcgtttga ccggttggct gaatgtaacc
    10501 tgccctcgcc caaaccggac cgtatttaaa tttggccgca gctttataca ggcttatacc
    10561 gcttttgtcg ccggaccagt cttcgtcata ggctttatta ctttttgaaa acgcgatttc
    10621 gttcgggtgg gagctgtcgc cgttttccgc catttcaatc gccgtaaacg cagcaatatc
    10681 aaggccgaac atatcagcag cataaccgga ctgaaaatcg aggttggcat tccaggtgga
    10741 gtgagaaagg ttggttttgt atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg
    10801 ctgccagtaa tagataccgc cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc
    10861 ctccggcatt gccataaagc ccgacattgc tgtaacaccg gcaatagcca gcgccagcgt
    10921 actacgtttg ccactaaacg tacgcatggg ttaatcctct ttgacgtata aattgctgca
    10981 ccaaaggtga acagcgaaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    11041 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    11101 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    11161 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    11221 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    11281 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    11341 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    11401 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    11461 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    11521 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    11581 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    11641 aatgctttac ccgcaaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    11701 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11761 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11821 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11881 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11941 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    12001 gcttcttcgc ggaaatcggc gcgatcgatc caaatttcac cgctaaacgg cacctgacgg
    12061 ctccccattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    12121 tagttttcga taaccagttt caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg
    12181 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    12241 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    12301 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    12361 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    12421 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    12481 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    12541 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    12601 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    12661 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12721 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12781 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12841 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12901 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12961 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    13021 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    13081 ttcggctccg gcgggaaacg ggtgtgtact gtgatgtgct taccactggc cagatcttca
    13141 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    13201 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    13261 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    13321 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    13381 gcataaagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    13441 ctgagcttta atgagcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    13501 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    13561 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    13621 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    13681 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13741 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13801 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13861 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13921 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13981 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    14041 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    14101 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    14161 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    14221 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    14281 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    14341 ggcggcaggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    14401 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    14461 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    14521 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    14581 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    14641 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    14701 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14761 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14821 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14881 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14941 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    15001 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    15061 ttctgggttg atggtcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    15121 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    15181 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    15241 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    15301 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    15361 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    15421 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gcatcgcaaa
    15481 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    15541 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    15601 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    15661 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15721 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15781 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15841 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15901 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15961 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    16021 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    16081 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    16141 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    16201 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    16261 aagaagtgat gattatggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    16321 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    16381 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    16441 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc
    16501 agggcaatat tttttaaaat cggggggcag aatgtatgca ttaacccagg gccggatctt
    16561 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    16621 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    16681 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16741 caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16801 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16861 cgtgcgcgtt atgcgtgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16921 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16981 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    17041 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    17101 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    17161 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    17221 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    17281 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    17341 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    17401 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    17461 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    17521 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    17581 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac
    17641 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    17701 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17761 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17821 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17881 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17941 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    18001 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    18061 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaagctg
    18121 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    18181 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagaa
    18241 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    18301 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    18361 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    18421 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    18481 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    18541 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    18601 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    18661 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18721 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18781 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18841 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18901 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18961 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    19021 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    19081 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    19141 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgct
    19201 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    19261 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttcct
    19321 acaactggga catgattcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    19381 ccaccaaccc ggacacccac gggcgcggtt tttatcccgc ttgtggcgcg ttgtgtgcag
    19441 ggattgagaa aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca
    19501 tccgcgcggc attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata
    19561 acctgcgtac cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt
    19621 ctggtgtttc atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc
    19681 cgtcagtcgc tgaaatcgac gttatctgaa taccacccac gtctgcggac gtggttggct
    19741 ctgatgacaa acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc
    19801 aatatattga attttcatgc ttttgtaggc cggataaggc gttcacgccg catccggcat
    19861 gaacaaatcg cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc
    19921 cgctaaaaat tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat
    19981 cgacaatcac cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc
    20041 tttgcggcat ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga
    20101 aggttatgtg ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta
    20161 agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt
    20221 atgccagcga taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg
    20281 gggcgcaacc tctctacaac caacaaaaaa accacgtact ggcggtaaac ggtgaaatct
    20341 acaaccacca ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg
    20401 actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc
    20461 agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg
    20521 accatctggg gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg
    20581 cctcagaaat gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga
    20641 gctatttgtg gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact
    20701 ttgatgcagt gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt
    20761 ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg
    20821 attcctcaat tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg
    20881 aacgctctga agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac
    20941 cggatcttaa agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc
    21001 acttcactgt acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt
    21061 atgatgtgac cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg
    21121 cgatgggcat taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc
    21181 tttacttcca taaagcgccc aacgttaaag aactgcatga agagacggtg cgtaaactgc
    21241 tggccttgca tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg
    21301 aagcacgcgt tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc
    21361 aggataaaat gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt
    21421 catacctgcc cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt
    21481 acagttggat cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg
    21541 aaactgcccg cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc
    21601 gcgagatctt tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc
    21661 cttccgtcgc gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg
    21721 acgatccgtc tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt
    21781 actgccccgg agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg
    21841 cagtaaggaa atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca
    21901 gtatttcata gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac
    21961 aaaagaatgg ctgtagggtg gggaaaaaca caaagtaaac aataattgac gaatatagcg
    22021 ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg
    22081 ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa
    22141 aaaaagatgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    22201 ggttcgaatc ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca
    22261 ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt
    22321 caccagaaag cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac
    22381 cggcattccc tggttcgaat ccaggtaccc cagccatctt cttcgagtaa gcggttcacc
    22441 gcccggttat tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct
    22501 ggttcgaatc caggtacccc agccatcgaa gaaataatct ggctacgtag ctcagttggt
    22561 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat
    22621 tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag
    22681 cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca
    22741 atttattcaa gacgcttaca tcgtaagtgc acccagttgg ggtatcgcca agcggtaagg
    22801 caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa
    22861 aagctcgctt cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg
    22921 acacgtctta tccttcaatg tcggatgcga cgctgccgcg tcttatccga cctacggctg
    22981 gcacgcatcc ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt
    23041 tcaacacgcc agcacgctct gccgccatta acccgagatt acgcacaaaa cgcagcggcg
    23101 gcagattatt actgaatccg gcataaaaca gatccatacc actttgcata atgaagttat
    23161 ccgccatgcg ccgcatctgg taacgcttga ggacaggata actggcccac gcttcaccgt
    23221 agctgcgggc attcaccaga acatcaatca gggcatcgac atcacgataa ccaagattca
    23281 ccccctgccc cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg
    23341 gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg
    23401 taacgtaacc cagacgcgac gggaaatgct tcgcgatttc cgcctggagt tgcgccatat
    23461 tcatattctg caactggcga atacgcgtcg gagagtcata ccacaccagc gatgcccagt
    23521 tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt aaattgctgc caggtgctgt
    23581 cgccgggatc gttctcacac tgtacgctaa tcaacatgca cgactgcgca tactgccagg
    23641 catgaacgcc aattcccgcc atctgccgca cctgagaatt tgcgccgtcg gcaccaatca
    23701 ccagtttcgc gcggatcgtt tcaccgccct tcagttccag ctcctgaaga tcattatctc
    23761 gatgtaatgc aatcagcgag gttggcacac gtaacgttac tttcggatgc gcttccagcg
    23821 cctgccacaa cgcctgttgc agcactgtgt tttccaccat gtagccaagc aacggcaact
    23881 ttaattcggc tgcgtcaaat accacatgtg ccgtttccca ctcccaggtt tccagtctgc
    23941 ggtaaggatg gcaacgcata gcctgtactg catcccagac ccctaaccct ttaagcaatg
    24001 ataccgaagc cgcgctgatc gccgagatcc gcacgtccgg ttggctgtca gcgacaaacg
    24061 gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg
    24121 ccagtgcgcc gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca
    24181 tggtcaatca tcctgttagc aatatgcttt aagtttacag gatttttacc cccaagggct
    24241 gataacgctc atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct
    24301 ggctactatt tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct
    24361 gtcagatgaa cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct
    24421 atcaactgac cgacgtggcg gaagaagcgg atgtgctact gctgaacacc tgctcaatcc
    24481 gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga
    24541 agaatccaga cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca
    24601 ttcgccagcg cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc
    24661 cggagatgat caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg
    24721 aaatcgagaa gtttgaccgt ctgccggaac cgcgcgccga agggccgacc gcgtttgtct
    24781 ccatcatgga aggctgcaat aaatattgca cctactgcgt ggtgccttac acccgtggtg
    24841 aagaggtaag ccgtccgtct gacgatattc tgtttgagat tgcccagctt gcggctcagg
    24901 gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg
    24961 acggcaccac cggatcgttt gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg
    25021 atcgtattcg ctttaccacc agccatccga tcgaattcac cgacgatatc atcgaagtgt
    25081 accgcgacac gcctgagctg gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc
    25141 gcattctgaa cctgatggga cgtacccata cggcgctgga gtacaaagcg atcatccgta
    25201 aactgcgtgc ggcgcgtccg gatattcaga tcagttctga cttcattgtt ggcttccctg
    25261 gcgaaaccac cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca
    25321 tgagctacag ctttatcttc tctgcacgtc caggaacgcc tgccgccgat atggttgatg
    25381 atgttccgga agaagagaag aagcagcgtc tgtatattct gcaagaacgt atcaatcagc
    25441 aagcgatggc atggagccgt cggatgctcg gcaccaccca gcgtattctg gtagaaggga
    25501 cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga aaataaccgc gtggtcaact
    25561 tcgagggcac accggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc
    25621 cgaactctct gcgcggtaaa gtggtgcgaa ctgaagatga aatgggtctg cgcatggcag
    25681 aaacaccgga gtcggtgatt gcccgcaccc gcaaagaaaa cgaccttggc gtgggttatt
    25741 atcagccgta attctcaggc ctgccgttcc ggtgggcctt gttttttctc tctttatccc
    25801 catctttatc gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg
    25861 gtgaatgttc agcttccggc ggcatacaga taagaggaac ggtttgaaca tagacactcg
    25921 cgaaatcacc ctggagccag cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga
    25981 tgacaacatc aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgataacca
    26041 ctttaaactg accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct
    26101 gtatgtcgat actgccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca
    26161 ccttgcgatt aaagaagcgc gggtactgga gcaaagcgcg gagagcgtgc cggagtacgg
    26221 caaagcggtc aatatcaaaa ctaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc
    26281 gcagtacatc gccaatattc tcgaccatga cattaccttc ggcgttggcc cggcgggtac
    26341 gggtaaaacc tacctggcag tggctgcggc agttgatgcc ctggagcgtc aggaaattcg
    26401 ccgtattctg ctgactcgtc cggcggtcga agccggtgag aaactgggct tcctgcctgg
    26461 cgatttaagc cagaaagtag acccgtatct gcgcccactg tacgacgcgc tgtttgaaat
    26521 gctgggcttt gagaaagtcg agaaactgat tgagcgcaac gttattgaag tcgcgccgct
    26581 ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc attctcgatg agagccagaa
    26641 cactaccatc gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt
    26701 tatcaccggc gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca
    26761 tgccattgaa gtgctggccg atgtcgaaga gatcagcttt aacttcttcc acagcgaaga
    26821 cgtggttcgt cacccggtgg tggcgcgtat cgttaacgct tatgaagcct gggaagaagc
    26881 agaacaaaaa cgtaaagcgg cgctggcagc agaacgcaag cgcgaagaac aggaacaaaa
    26941 atgagtcagg tgatcctcga tttacaactg gcatgtgaag ataattccgg gttaccggaa
    27001 gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga agagtcggaa
    27061 gtgactattc gcgtggtcga taccgccgaa agccacagtc tgaatctgac ctatcgcggt
    27121 aaggataagc cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggagatg
    27181 tcgctactgg gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa
    27241 ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc acggcagtct gcatttgtta
    27301 ggttacgatc acattgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt
    27361 atgcttgctc tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat
    27421 ttgactggca gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg
    27481 agaacccata cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca
    27541 agaagggatt tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg
    27601 acgaactgct ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc
    27661 gcgatatgct cgaaggggtg atggacatcg cagaccaacg cgttcgcgac atcatgatcc
    27721 cccgctccca gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca
    27781 tcatcgagtc cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag
    27841 ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca
    27901 tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc
    27961 tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg
    28021 tttccggtct ggtgaccatt gaagacatcc tggaactgat tgttggtgag attgaagacg
    28081 agtatgacga agaagatgat atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc
    28141 gcgcactggc ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag
    28201 aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg gcatcttccg gcgcgtggcg
    28261 aaactatcga catcgacggt taccagttca aagtggcgat ggccgatagt cggcgtatta
    28321 ttcaggttca tgtcaaaatc ccggatgact caccccagcc aaagctggat gaataaaacc
    28381 gaaactggat agataactac atggattttg cctcattaat tgaacgccag cgcattcgcc
    28441 tgctgctggc gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgacgtct
    28501 ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt gacctttaac cgtcgcccac
    28561 tccagtctgc cgctattggc ttttgctggg gatttggcct ctttggcagc ggtattaact
    28621 gggtctatgt cagcatcgcg acctttggcg ggatgcctgg cccggttaac atcttcctgg
    28681 tggtgctgct ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc
    28741 gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc cacccctgct ctctggcaag
    28801 tgaccgagtt tctgcgcggt tgggtgctga ctggcttccc gtggttacag ttcggctata
    28861 gccagattga tggcccgtta aaagggctgg caccgctaat gggcgtggaa gccattaact
    28921 tcctgctgat gatggttagt ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc
    28981 cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc gctgcgttac atccaatggt
    29041 ttaccccgca accggagaaa accattcagg tttcgatggt tcagggcgat attccgcaat
    29101 cgctgaaatg ggacgaaggc cagcttctta atacgctaaa gatttactac aacgcaacgg
    29161 caccgctgat gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg
    29221 aaattaatca gcaaccgttc ctcaaagcac tggacggtga gttgcgtgat aaaggtagct
    29281 cgctggtaac cgggattgtc gacgcgcgtc tcaataagca gaaccgctac gatacctaca
    29341 acaccatcat cacgctgggt aaaggtgcac cgtacagcta cgaatcagcc gatcgctata
    29401 acaaaaacca tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt
    29461 tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccgc
    29521 cgctgtcggc aaatggtatt gagcttactg cggctatttg ctacgagatt attctcgccg
    29581 agcaagtgcg cgataacttc cgcccggata ccgactacct gctgactatc tccaacgatg
    29641 cgtggtttgg taaatctatt ggtccatggc aacacttcca gatggcgcga atgcgtgcgc
    29701 tggagctggc gcgcccactg ttgcgcagca ccaacaacgg cattacggcg gtgattggcc
    29761 cgcagggtga gattcaggcg atgatcccgc agttcgcccg cgaggtgtta accactaacg
    29821 tgacgccgac caccggactc acaccatacg cacgtaccgg caactggccg ctgtgggtgc
    29881 tgacggcact gtttggtttt gctgctgtgt tgatgagtct gcgtcagcga cgtaaataat
    29941 cccttcttat cgtgccggaa tacatcccat atttcggcac gctcaccgga tgcggcatca
    30001 gtgcattatc cggataacaa tattctccta tccaactttg gcacatctat tgctttgtta
    30061 tacaaggcaa agcttgaacc agcatcagta caacccggtt gcaccacagc aatccaccgg
    30121 tagcaccaaa agagtgcatt ttaagaattt tgcttcagaa tggtgcggtg cacctcgcaa
    30181 aaacaaacaa taaaacatca atatctttac atttagccaa actaaatgtt acctaacagg
    30241 cacaacactg cacaataaag ttgcagacga taacaacaca aacactcaca acgggtatcc
    30301 atgcgttctt aacgcagaag ataaaggtgt tggatatgca attacgtaaa cctgccacag
    30361 caatcctcgc cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag
    30421 caggcagtac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat
    30481 cttcagtacc tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt
    30541 actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa
    30601 aactgattcc gattacctca caaaatcgta ttccactgct gcaaaacggc actttcgatt
    30661 ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgaca
    30721 ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg cggcgatatc aaagattttg
    30781 ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca
    30841 acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg
    30901 actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat gacgctctgc
    30961 tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc
    31021 agtctcagga agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga
    31081 tggatgacac tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat
    31141 ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa
    31201 tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg
    31261 cggaaattcc agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg
    31321 ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta
    31381 acgctgctac cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt
    31441 tttttacaac aagccccgtt cggcaatacc acctatcttg gttgggtctg gagtggtttt
    31501 caggtgacga tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt
    31561 ttcggcattt tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc
    31621 gaactgttcc gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg
    31681 gagctgctgc cggagaaaat cggcatgtgg tttaaggccg agctggatcc caatattcag
    31741 tttttccttt catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag
    31801 gtacgcgccg ctattcagtc gctaccgcgt gggcagaaga atgccgcgct ggcgatgggc
    31861 ctgacgctac cgcagactta tcgatacgta ctgctgccta atgcttatcg cgttatcgtc
    31921 ccgccgatga cttccgagat gatgaacctg gtgaaaaact ccgccatcgc ctcgaccatc
    31981 ggtctggtgg atatggcggc gcaggcgggt aaattgctcg actactcggc tcacgcctgg
    32041 gagtcgttta ccgccatcac gcttgcgtat gtgttgatta acgcttttat catgctggtg
    32101 atgacgctgg ttgaacgtaa agttcgcctg cccggcaaca tggggggcaa ataatgtacg
    32161 agtttgactg gagttccatt gttccttccc tgccatatct gctcgacggg ctggtgatca
    32221 ccctgaaaat caccgtcacg gcagtcgtga ttggtatttt gtggggtacc atgctggcgg
    32281 tgatgcgctt gtccagcttc gcgcctgttt cgtggtttgc caaagcctac gttaacgtat
    32341 tccgctcgat tcctttagtc atggttttgc tgtggtttta cttgattgtg ccgggatttc
    32401 tgcaaaatgt gctgggatta tcgccaaaaa atgatattcg tttaatttcg gcgatggtgg
    32461 cgttttcgat gtttgaagcg gcctactatt cagagattat ccgtgccggt attcaaagta
    32521 tttcccgtgg tcagtcgagc gccgcgctgg cgttggggat gactcactgg cagtcgatga
    32581 aactgattat tctgccgcag gctttccgcg cgatggtgcc gctgctgctc actcagggca
    32641 tcgtactgtt ccaggatacc tcgctggtgt atgtgttatc tctggccgat ttcttccgta
    32701 ccgcctcaac cattggtgag cgtgatggta cgcaggtcga gatgatcctg tttgccggat
    32761 ttgtttattt cgttattagc cttagcgcgt cgttgttggt cagctacttg aaaagaagga
    32821 cagcataatg attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac
    32881 cgactgctca accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc
    32941 cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat
    33001 caccgtcgat ggtatcgtgg ttaacgacaa gaaaaccgat ctggcaaagc tgcgctcccg
    33061 cgtcgggatg gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct
    33121 gaccctggcg caggtgaaag tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt
    33181 gaaattgctg gagcgcgtgg gactttccgc acatgccaat aaatttccgg cgcagctttc
    33241 cggcggtcag cagcagcgcg tggcaatcgc tcgcgcattg tgtatggctc ctattgcgat
    33301 gctgttcgac gaaccgacat cggcgctgga tccggagatg atcaacgaag tgctggacgt
    33361 gatggtggaa ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt
    33421 tgcccgcaaa gtggcgaatc gggtgatctt tatggacgag ggtaaaattg tcgaagactc
    33481 gccgaaagac gctttcttcg atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa
    33541 aatcctgcat taatcactct ggcgcacata cccaattgcg cgccatctca cgctttatcg
    33601 tttcgcccct cgtatctgtt acatcctggc gttacccttg tcgcaaagaa gcacacaaca
    33661 aggagcaaca atggcactgc caattctgtt agattgcgac ccaggtcatg atgacgctat
    33721 cgcaatagtt ctcgcactcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc
    33781 cggaaaccag acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa
    33841 tcgcaccgat attccggtgg caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat
    33901 cgcggacaat gtgcacggcg aaagcggtct cgacggcccg gcattaccgg aaccgacgtt
    33961 cgcaccgcaa aactgtacgg cggtggagct gatggcgaaa acgctgcgcg aaagtgcgga
    34021 acctgtcacg attgtttcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca
    34081 tccggaactg catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg
    34141 taactggacg cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt
    34201 cttccagtca gggatcccgg tggtgatggc cggtctggat gttactcata aagcgcaaat
    34261 ccacgttgaa gacaccgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc
    34321 cgaactgctg gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggcgc
    34381 accgctgcat gacccatgca ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt
    34441 tgagcgctgg gttggcgtgg aaacacaggg gaaatatacc cagggtatga cggttgttga
    34501 ttattattat ctgactggca ataaaccgaa tgccactgta attgtcgatg ttgatcgtca
    34561 gggctttgtt gatttactgg ctgatcgtct gaaattttac gcttaacact cgtcattgat
    34621 taagatcacc acgcgttacc tgggtggtct taatctattt atctcttttt ctttcaagat
    34681 ataaatgcaa tggataatgc agaactcgcc ataggtatcg atctcggcac taccaatagt
    34741 ttaattgccg tctggaaaga cggtgccgcg caattaattc caaataagtt cggtgaatat
    34801 ttaacaccat ccataattag catggatgaa aataatcata ttttagtcgg aaaaccggct
    34861 gtatcacggc gtacttcgca tccggataaa acggcagcgt tatttaaacg tgcaatgggc
    34921 agtaatacca actggcggtt aggcagcgac acatttaacg cgccagaact gtcctctttg
    34981 gtattacgct cattaaaaga agatgccgaa gaatttctgc aacgtccgat taaagatgtg
    35041 gtgatctccg ttctggctta tttcagcgat gaacaacgca agcatacccg tttagcagcg
    35101 gacttagccg ggttaaatgc ggtacgctta attaatgaac ccacagcagc tgcgatggcg
    35161 tatggcctgc atacccaaca aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg
    35221 tttgacgtta cggtgcttga gtacgccacg ccggtgattg aagttcacgc ctccgctggc
    35281 gacaacttcc tcggtggcga agattttacc catatgttgg tcgatgacgt tttaaagcgc
    35341 gcggatgtca caaggaccac gcttaacgag agtgaactgg cagccttgta cgcctgtgtg
    35401 gaagcggcaa aatgtagcaa tcaatcgcca atgcacattc gctggtagta ccaggatgaa
    35461 acgcgggaat gcgaatttta cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat
    35521 cgcttgcgag tgccgattga acaggcgttg cgcgatgcgc gtctgaagcc gagtcaaatc
    35581 gacagtctgg tgctggttgg cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg
    35641 cgtctgtttg gcaaattacc gtatcaaagt tacgatccga gcaccattgt cgcgctgggc
    35701 gcagcaatcc aggccgcctg ccgcttacgc agtgaagata ttgaagaagt gatcctcact
    35761 gatatttgcc cttactcgtt gggcgttgag gttaaccgcc agggcgtttc cggcattttc
    35821 tcgccgatta ttgaacgaaa caccactgtg cccgtgtcgc gtgtagaaac ttattcaacc
    35881 atgcacccgg aacaggattc aattacggtt aacgtctatc agggagaaaa ccacaaagtt
    35941 aaaaacaaca ttctggtgga atccttcgat gtgccgttga agaaaaccgg ggcttatcag
    36001 tcgattgata ttcgctttag ttatgatatc aacgggttgc ttgaagttga cgtgcttctg
    36061 gaagacggca gcgttaagtc cagagtgatt aaccacagcc cggtaacatt gagcgcgcag
    36121 cagattgaag agagtcggac gcggttatcc gcattgaaaa tttatccgcg cgatatgctc
    36181 atcaatcgca cctttaaagc caaactggaa gagttgtggg cgcgggcgct gggtgacgag
    36241 cgagaagaga ttggccgggt gatcaccgat tttgatgcgg cgctgcagtc aaacgatatg
    36301 gcccgcgtcg atgaggtcag gcggcgggcg agcgtttatt tagccattga gacctcataa
    36361 tcacggctgc cggatgcggc gtaaatgctg catccggcat cggcgcaaaa aacacatcgt
    36421 caactaaaca acatcgcgat tttgccgaga gcataaccta taaccagcac cgttaaactg
    36481 attttgataa tgctggtaat gctctgtggt ttttcaccct catcattttt ttcttcatgg
    36541 ttttcaggtg ttggctcatc gacatcttca tcctccggct cgagttttac tttcgtcaac
    36601 agcatccggg tgataatggc ctctaatgga tgagcatctc cgccttccag ccttgtatta
    36661 agacgggcca gcatctcttg tttcggaaac gcttcaagta ctacctgttc cgctacccga
    36721 tacaaatcca gtaacggtga atcttcttct gtctcgctac cgactctcga tttccaccac
    36781 gcccatgctt tgcgtttgaa gaataacgac tcaataaacg attgacctaa cggcgataat
    36841 tttccgctat gtaaaatttc cgcccataac agccgcgtcc gggcgctctc accaggaaca
    36901 tatttcagcg gttgaccttg cgcatctacc atgctccgcg cttcgataac catcactaat
    36961 aacgcgaaat aatccgggca atgacgcttg ctccactgca acaacaaatt atcagcttgt
    37021 gtagatgggt aactttccca gtacgcgccg agatcagcaa gcaggctttc ctcctcgccg
    37081 cagtacaaac gttgtgcaca ctggtaatac cgggcatctt cgctttctgg ttcttccgct
    37141 tgccactgtt tggctacagg aatgagttcc accatacctg tttgcaccga gctgtaccag
    37201 cgcaacagtt tgcgatgtaa ctttgcatca tcaggaatga accacggtcc ttccatcgcc
    37261 aaaaacgcag tgacatagtc aggatggtaa cggcagcaac gttccagcgc gaagtaatat
    37321 tcaacagtct ggttctgtaa cgcgacggga tattccagaa ggctaaggaa atcgaaacaa
    37381 tcaccagcct gaatggcctc aagaaaagcc tccctttctt cctcatccat tccttcgctg
    37441 tcattaaacg aatgccagtt caaacgctct gccaacaaag aagcacaatt taatgacaga
    37501 tatcgcgctt ctatagcgat tgcacacagc ggccagcgta attgatcgac atcctccatg
    37561 ttccaggtat ttaattgctg aataaatttc tgccaggcgg agggctgaaa acgatcactt
    37621 tctgaatcca gtaatgtcct gaatgcacgt agtatttcat gttcagcggt ggcatctttt
    37681 tcttcatcat ctgcttcctc gacaggattt accgccagtc gcagcgcctc ttcataggcc
    37741 tggcgtaatg ctttaaaccc ctgcggatcg gtttcgggat gacacaacgg taagcgagca
    37801 agataagcct ggcggataat gtctatttgc gtcgtgcttt caatttcgag gatttgccaa
    37861 catgttttca taggtcgctc acatccttaa gcgttaccgg gcattcaggc tggacagtta
    37921 atggctgcca gggaagcgtg gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca
    37981 tccgactccc ggcgctatcg tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt
    38041 cttcgcactg agaagattgc cagtagagac gccctagtga ataggcagaa aaatagtgca
    38101 tccagctgtg atagtaatgg tgagctcgtg catagacgcg cgattgcagc cataaactct
    38161 cttcttccgt cagccagttg ttgagcacac cggcccgtga cagaaatccc attcgaacat
    38221 aatcccaggc tttgattccc ccttctccac agcacatagc ggtttgggca acgaattggg
    38281 tataaatcaa ccctctctcc gagccaccag caattaatgc tttccactct tcaggggaag
    38341 agcgttgcca tgtcagatat aagccagcta aatcattggc atgaccatca tcagtcatct
    38401 gataaatagt ctgaattaaa tcatcacgat tatttattcc ccaggatgat gagagatcga
    38461 attcttttat cccggtatac atttttggcg agtcatgcgc gggtttgcaa ctctgattaa
    38521 gtatttccat gggcgcagaa agcgcaaaaa gaagatattg ttcttcctta ttcatttttt
    38581 aataatccct ta
//
LOCUS       DAZPDE010000020        37783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPDE010000020 DAZPDE010000000
VERSION     DAZPDE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.020"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..74)
                     /locus_tag="ACHU0J_000958"
     CDS             complement(<1..74)
                     /locus_tag="ACHU0J_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR1215752.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHU0J_000959"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHU0J_000959"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1215753.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACHU0J_000960"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACHU0J_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1215754.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHU0J_000961"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHU0J_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1215755.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACHU0J_000962"
     CDS             2704..2940
                     /locus_tag="ACHU0J_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1215756.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACHU0J_000963"
     CDS             2943..3800
                     /locus_tag="ACHU0J_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1215757.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACHU0J_000964"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACHU0J_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHU0J_000965"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHU0J_000965"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1215758.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACHU0J_000966"
     CDS             complement(6415..7311)
                     /locus_tag="ACHU0J_000966"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1215759.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACHU0J_000967"
     CDS             complement(7360..7599)
                     /locus_tag="ACHU0J_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1215760.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACHU0J_000968"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACHU0J_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1215761.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACHU0J_000969"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACHU0J_000969"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1215762.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACHU0J_000970"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACHU0J_000970"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1215763.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACHU0J_000971"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACHU0J_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1215764.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACHU0J_000972"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACHU0J_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /locus_tag="ACHU0J_000973"
     CDS             complement(15268..16476)
                     /locus_tag="ACHU0J_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1215765.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16499..16909
                     /gene="tnpA"
                     /locus_tag="ACHU0J_000974"
                     /pseudo
     CDS             16499..16909
                     /gene="tnpA"
                     /locus_tag="ACHU0J_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACHU0J_000975"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACHU0J_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1215766.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="ACHU0J_000976"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="ACHU0J_000976"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1215767.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="ACHU0J_000977"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="ACHU0J_000977"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1215768.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="ACHU0J_000978"
     CDS             complement(19387..20556)
                     /locus_tag="ACHU0J_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1215769.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACHU0J_000979"
                     /gene_synonym="ynhF"
     CDS             complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACHU0J_000979"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1215770.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="ACHU0J_000980"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="ACHU0J_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1215771.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACHU0J_000981"
     CDS             complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACHU0J_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1215772.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /gene="punC"
                     /locus_tag="ACHU0J_000982"
     CDS             23177..24388
                     /gene="punC"
                     /locus_tag="ACHU0J_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1215773.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="ACHU0J_000983"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="ACHU0J_000983"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1215774.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACHU0J_000984"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACHU0J_000984"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1215775.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="ACHU0J_000985"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="ACHU0J_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1215776.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="ACHU0J_000986"
     CDS             complement(28137..29393)
                     /locus_tag="ACHU0J_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1215777.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="ACHU0J_000987"
     tRNA            29701..29777
                     /locus_tag="ACHU0J_000987"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="ACHU0J_000988"
     tRNA            29782..29858
                     /locus_tag="ACHU0J_000988"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="ACHU0J_000989"
     CDS             29966..30271
                     /locus_tag="ACHU0J_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1215778.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..31819
                     /locus_tag="ACHU0J_000990"
                     /pseudo
     CDS             30397..31819
                     /locus_tag="ACHU0J_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACHU0J_000991"
     CDS             complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACHU0J_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1215779.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACHU0J_000992"
     CDS             complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACHU0J_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1215780.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33429..34097)
                     /locus_tag="ACHU0J_000993"
     CDS             complement(33429..34097)
                     /locus_tag="ACHU0J_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1215781.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34161..34799)
                     /locus_tag="ACHU0J_000994"
     CDS             complement(34161..34799)
                     /locus_tag="ACHU0J_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1215782.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34812..36913)
                     /locus_tag="ACHU0J_000995"
                     /pseudo
     CDS             complement(34812..36913)
                     /locus_tag="ACHU0J_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36934..37560)
                     /locus_tag="ACHU0J_000996"
     CDS             complement(36934..37560)
                     /locus_tag="ACHU0J_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1215783.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9067 a         9889 c         9293 g         9534 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgct
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtaaagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat
    31621 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    31681 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    31741 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    31801 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    31861 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    31921 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga
    31981 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    32041 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    32101 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    32161 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    32221 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    32281 gctataaccc atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc
    32341 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    32401 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    32461 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    32521 ttcctcaatc agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    32581 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    32641 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    32701 ccccgtggtg caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc
    32761 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    32821 atagagacac aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac
    32881 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    32941 ttccccctgc ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca
    33001 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    33061 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    33121 aaccgcagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    33181 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    33241 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    33301 cagcaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    33361 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    33421 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    33481 ggttttccgg caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga
    33541 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    33601 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    33661 ttagtgacag ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag
    33721 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc
    33781 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    33841 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    33901 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    33961 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    34021 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    34081 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    34141 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    34201 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    34261 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    34321 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    34381 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    34441 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    34501 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    34561 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    34621 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    34681 tcagcaggtt ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    34741 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    34801 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    34861 agccccagac gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    34921 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    34981 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    35041 tcattacgca tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag
    35101 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    35161 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    35221 gtcattggcc agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc
    35281 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    35341 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    35401 ccggaaccaa taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta
    35461 acaatggaac cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc
    35521 gaccagagtt tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg
    35581 atggcatatg aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag
    35641 taaaaatctt taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca
    35701 tactcttcag ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg
    35761 cgatgcaact gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc
    35821 aggttaccaa tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag
    35881 atggtggtat ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac
    35941 tctttcaccc gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg
    36001 ctcattttta ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta
    36061 cgaaagccga ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct
    36121 tcagccgcgc cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgaatac
    36181 tctgcccacg attgtggcgt acttggcacg acatggttgt tattcgcacc aataagttca
    36241 gtcatcatgt aatcattgag acgcttcatc tcctgacgat cggcaatgtt gacccctttc
    36301 gtcccttcaa ccgcaatcgc tttcaggttt ttcgaaccca ttattgcgcc agttcccgca
    36361 ccgccgctgt ggttacggct attcaacatg ccagagagag gaataaggtt ttccccagcc
    36421 tgaccaatag ccgccacaca ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt
    36481 gcgcgcgtcc cttttcccca taagaaatcg gctttttcca ggctaacttt gtcatcttta
    36541 atcttcagcc ataccggtga tttcgccttc ccttcgataa taatgacgtc gtatccagcg
    36601 aatttcattt gcgctgcaaa aaagccaccc atatgggcat cgcgactaaa tttcctttgg
    36661 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccaggta
    36721 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctagcggta
    36781 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    36841 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    36901 aaccgttagc catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat
    36961 aatttttaac gcccctgttg ggcaggcgtt tgcgcattca ccgcataaca cacatttcga
    37021 ggatttttta ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc
    37081 gctacagcca atacaacgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt
    37141 aatcgcacca atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc
    37201 cgcggtatag ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa
    37261 gaaataattg cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa
    37321 gttggtacaa gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag
    37381 cacccctttg ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca
    37441 acccaaaagt gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat
    37501 acgcaagaat tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat
    37561 ttatttcgca tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat
    37621 tcaactaata cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg
    37681 tatgagaaaa gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt
    37741 gattattctg agcccttaac attgatcgtt atcaattaaa att
//
LOCUS       DAZPDE010000021        36822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPDE010000021 DAZPDE010000000
VERSION     DAZPDE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.021"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            136..492
                     /locus_tag="ACHU0J_000997"
     CDS             136..492
                     /locus_tag="ACHU0J_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1215784.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            496..1623
                     /gene="dapE"
                     /locus_tag="ACHU0J_000998"
     CDS             496..1623
                     /gene="dapE"
                     /locus_tag="ACHU0J_000998"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1215785.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1651..1851
                     /locus_tag="ACHU0J_000999"
     CDS             1651..1851
                     /locus_tag="ACHU0J_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1215786.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1961..2659)
                     /gene="ypfH"
                     /locus_tag="ACHU0J_001000"
     CDS             complement(1961..2659)
                     /gene="ypfH"
                     /locus_tag="ACHU0J_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1215787.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2733..4748)
                     /gene="tmcA"
                     /locus_tag="ACHU0J_001001"
     CDS             complement(2733..4748)
                     /gene="tmcA"
                     /locus_tag="ACHU0J_001001"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1215788.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4763..5626)
                     /locus_tag="ACHU0J_001002"
     CDS             complement(4763..5626)
                     /locus_tag="ACHU0J_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1215789.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5794..6507)
                     /gene="purC"
                     /locus_tag="ACHU0J_001003"
     CDS             complement(5794..6507)
                     /gene="purC"
                     /locus_tag="ACHU0J_001003"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1215790.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6720..7754)
                     /gene="bamC"
                     /locus_tag="ACHU0J_001004"
     CDS             complement(6720..7754)
                     /gene="bamC"
                     /locus_tag="ACHU0J_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1215791.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7771..8649)
                     /gene="dapA"
                     /locus_tag="ACHU0J_001005"
     CDS             complement(7771..8649)
                     /gene="dapA"
                     /locus_tag="ACHU0J_001005"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1215792.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8795..9367
                     /locus_tag="ACHU0J_001006"
     CDS             8795..9367
                     /locus_tag="ACHU0J_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1215793.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9367..9837
                     /gene="bcp"
                     /locus_tag="ACHU0J_001007"
     CDS             9367..9837
                     /gene="bcp"
                     /locus_tag="ACHU0J_001007"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1215794.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10090..10707
                     /gene="hyfA"
                     /locus_tag="ACHU0J_001008"
     CDS             10090..10707
                     /gene="hyfA"
                     /locus_tag="ACHU0J_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1215795.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10707..12724
                     /gene="hyfB"
                     /locus_tag="ACHU0J_001009"
                     /pseudo
     CDS             10707..12724
                     /gene="hyfB"
                     /locus_tag="ACHU0J_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12735..13682
                     /gene="hyfC"
                     /locus_tag="ACHU0J_001010"
     CDS             12735..13682
                     /gene="hyfC"
                     /locus_tag="ACHU0J_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1215796.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13699..15138
                     /gene="hyfD"
                     /locus_tag="ACHU0J_001011"
     CDS             13699..15138
                     /gene="hyfD"
                     /locus_tag="ACHU0J_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR1215797.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15150..15800
                     /gene="hyfE"
                     /locus_tag="ACHU0J_001012"
     CDS             15150..15800
                     /gene="hyfE"
                     /locus_tag="ACHU0J_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1215798.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15805..17397
                     /gene="hyfF"
                     /locus_tag="ACHU0J_001013"
     CDS             15805..17397
                     /gene="hyfF"
                     /locus_tag="ACHU0J_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1215799.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17387..19101
                     /gene="hyfG"
                     /locus_tag="ACHU0J_001014"
                     /pseudo
     CDS             17387..19101
                     /gene="hyfG"
                     /locus_tag="ACHU0J_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19111..19656
                     /gene="hyfH"
                     /locus_tag="ACHU0J_001015"
     CDS             19111..19656
                     /gene="hyfH"
                     /locus_tag="ACHU0J_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1215800.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19653..20411
                     /gene="hyfI"
                     /locus_tag="ACHU0J_001016"
     CDS             19653..20411
                     /gene="hyfI"
                     /locus_tag="ACHU0J_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1215801.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20404..20817
                     /gene="hyfJ"
                     /locus_tag="ACHU0J_001017"
     CDS             20404..20817
                     /gene="hyfJ"
                     /locus_tag="ACHU0J_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1215802.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20847..22859
                     /gene="hyfR"
                     /locus_tag="ACHU0J_001018"
                     /pseudo
     CDS             20847..22859
                     /gene="hyfR"
                     /locus_tag="ACHU0J_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22881..23753
                     /gene="focB"
                     /locus_tag="ACHU0J_001019"
                     /pseudo
     CDS             22881..23753
                     /gene="focB"
                     /locus_tag="ACHU0J_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23847..24908)
                     /locus_tag="ACHU0J_001020"
     CDS             complement(23847..24908)
                     /locus_tag="ACHU0J_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1215803.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25121..26584
                     /gene="bepA"
                     /locus_tag="ACHU0J_001021"
     CDS             25121..26584
                     /gene="bepA"
                     /locus_tag="ACHU0J_001021"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1215804.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26605..26964
                     /gene="arsC"
                     /locus_tag="ACHU0J_001022"
     CDS             26605..26964
                     /gene="arsC"
                     /locus_tag="ACHU0J_001022"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1215805.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(27097..27798)
                     /locus_tag="ACHU0J_001023"
     CDS             complement(27097..27798)
                     /locus_tag="ACHU0J_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1215806.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27893..29182)
                     /gene="uraA"
                     /locus_tag="ACHU0J_001024"
     CDS             complement(27893..29182)
                     /gene="uraA"
                     /locus_tag="ACHU0J_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1215807.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29268..29894)
                     /gene="upp"
                     /locus_tag="ACHU0J_001025"
     CDS             complement(29268..29894)
                     /gene="upp"
                     /locus_tag="ACHU0J_001025"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1215808.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30218..31255
                     /gene="purM"
                     /locus_tag="ACHU0J_001026"
     CDS             30218..31255
                     /gene="purM"
                     /locus_tag="ACHU0J_001026"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1215809.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31255..31893
                     /gene="purN"
                     /locus_tag="ACHU0J_001027"
     CDS             31255..31893
                     /gene="purN"
                     /locus_tag="ACHU0J_001027"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1215810.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            32065..34131
                     /gene="ppk1"
                     /locus_tag="ACHU0J_001028"
     CDS             32065..34131
                     /gene="ppk1"
                     /locus_tag="ACHU0J_001028"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1215811.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34136..35677
                     /gene="ppx"
                     /locus_tag="ACHU0J_001029"
     CDS             34136..35677
                     /gene="ppx"
                     /locus_tag="ACHU0J_001029"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1215812.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35716..>36822)
                     /gene="pdeF"
                     /locus_tag="ACHU0J_001030"
     CDS             complement(35716..>36822)
                     /gene="pdeF"
                     /locus_tag="ACHU0J_001030"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1215813.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8535 a         9724 c         9565 g         8998 t
ORIGIN      
        1 aaaatgttta atattattta atagttgtta atttgaatac ttcgataatg ttatattttc
       61 tgataatcat ttgcaggcaa aatgttttca cccttaaatg agtatttatt ctcataaatc
      121 gaaaaaggat tcattatggt tacactttac ggcatcaaaa attgtgacac cattaaaaag
      181 gctcgccgtt ggctggaagc caataacatc gactatcgtt ttcatgatta ccgcgtcgat
      241 gggctggaca gcgaattatt gaacggtttt atcaacgaat taggctggga agcgttactc
      301 aacacccgtg gtacaacctg gcgtaaactg gacgaaacta cccgcaataa aatcaccgat
      361 gcggcctctg cggcggcatt aatgactgaa atgcctgcaa ttatcaaacg tccattgctc
      421 tgcgcgcctg gtaagcctat gctgctgggt ttcagtgatt ccagttatca gcaatttttc
      481 catgaggtgt agtctatgtc gtgcccggtt attgagctga cacaacagct tattcgccgc
      541 ccttccctga gtcctgatga cgcaggatgt caggctttgt tgattgaacg tttgcaggcg
      601 atcggtttta ccgttgaacg catggatttt gccgatacgc agaatttttg ggcatggcgt
      661 gggcaaggtg aaacgttagc ctttgccggg cataccgacg tggtgccgcc tggcgacgcc
      721 gatcgttgga tcaatccgcc gtttgaaccc accattcgtg acggcatgtt attcgggcgc
      781 ggtgcggcag atatgaaagg ctcgctggcg gcgatggtgg tagcagcaga acgttttgtc
      841 gcacaacatc ccaaccatac ggggcgactg gcatttctga tcacttctga tgaagaagcc
      901 agtgcccaca acggtacggt aaaagtcgtc gaggcgttaa tggcacgtaa tgagcgtctc
      961 gattactgcc tggtcggcga accgtcgagt atcgaagtgg taggtgatgt ggtgaaaaat
     1021 ggtcgtcgcg gatcgttaac ctgcaacctc accattcatg gcgttcaggg gcatgttgcc
     1081 tacccacatc tggctgacaa tccggtacat cgcgcagcac ctttccttaa tgaattagtg
     1141 gctattgagt gggatcaggg caatgaattc ttcccggcga ccagtatgca gattgccaat
     1201 attcaggcgg gaacgggcag taacaatgtt attccgggtg aactgtttgt gcagtttaac
     1261 ttccgcttca gcaccgaact gactgatgag atgatcaaag cgcaggtact tgccctgctt
     1321 gaaaaacatc aactgcgcta tacggtggat tggtggcttt ccgggcagcc atttttgacc
     1381 gcgcgcggta aactggtgga tgcggtcgtt aacgcggttg agcactataa tgaaattaaa
     1441 ccgcagctac tgaccacagg cggaacgtcc gacgggcgct ttattgctcg catgggggcg
     1501 caggtggtgg aactcgggcc ggtcaatgcc actattcata aaattaatga atgtgtgaac
     1561 gctgccgacc tgcagctact tgcccgtatg tatcaacgta tcatggaaca gctcgtcgcg
     1621 tgatgagtgg ttctgcaaga ggaaataagc atggactggc tggctaaata ttggtggatt
     1681 ctggtgattg tctttttggt aggcgtcctg ctgaacgtga ttaaagatct caagcgcgtc
     1741 gaccataaga aatttctcgc caacaagccg gagcttcccc cgcatcgtga tttcaacgat
     1801 aagtgggacg atgacgacga ctggccgaaa aaggatcaac cgaagaagta agctgagtgc
     1861 tcaaccgtag gccggataag gcgttaacgc cgcatccggc aatggtgaac gatccctgat
     1921 gcgacgctca cgcgtcttat caggccaggt aatctgtctt ttacatcatt tcaatcacat
     1981 catcatcgcc aggcttaccg ccacttagcg cttggtcgaa ataatgtttc ggaatggtat
     2041 aacgcaagtg atccagggca aattgcatac tgcgattgtc gatcgcatgg cctaaatcct
     2101 ccacgatatc cagcgtcacg tcaccaccgg cactgattaa cgcttcctgc gccgcaactg
     2161 catgcgccag atcgataacc ggatcttcac caccatgaat caagtgaatt gtcgtggctg
     2221 tagacgcggt ctccggcagg ctcgcatagc gtccgttaaa ggcaatgacg cgcgaagcaa
     2281 ggcccggttc ggctttaatg ctctccagcg ccataatcgc gccttgtgag aaaccaatta
     2341 gcgccgtggc gttagcccct actccgctct gtttctgcca gtagcgtacc gtttcaataa
     2401 acgtcggcat gatggcatcc acacgcgcct ggcgattatc ttccgtaata ccctgcactg
     2461 aaaaccactg acgccccgcc ggattaccgc tcggctccgc gccaccgaca ctcaccacca
     2521 gcgcatcagg aaacagcggt gcaaaccagc tgcctatttc ccccattgcc accgggttat
     2581 ccccgacacc gtggaaaagc agtaacaact gttgtgcagg tttatccggg ctttgaacaa
     2641 caaaatggtc atgtttcatg gcgaactcct taactgatgt cattaatttt acgccgctga
     2701 ctcacaatga ccatgggaga aaattgaagg agtcagtgaa aaaattgcca ttgcgttatg
     2761 cgatcgcgca gacgctcagt gcgaacctca tttagtgcga acagcgcctg cgcggcctct
     2821 tcccgctggc gaaccagtaa catcttgcgg cctgaaagtt taagtgtggt acataactgc
     2881 gcgtcactgg cgtttttctg taaacgccca cgcagcgccg gtaatgccag ttcactggtt
     2941 tgtagcagac gcaataagca acctaacgat gttaatagcg gacgatgagc gaaagcaaaa
     3001 ccggccagtt caagccagtc gtcgtcagaa aggatggcat cgtttagtgg atcaacagga
     3061 agcatttcgc cattccactg cgcgagagct tgcgcatcgc gacgtaaacg gtaatgctca
     3121 cgttcagcca gctgtttacc cgcatcactc atcggtaaca gcgccatcgc cgtatagcaa
     3181 ccgctgctgg cttcccgatg attacccatc cgcaccagca caaaaccgca gcgttgccag
     3241 aaacgccata actccccagt gtaaccaaaa ctcaccgaaa gatagtcgag gtcatgcgta
     3301 tattgcaaag ctccagcaat aagttgtcgc cctgtgcctt cccgctgacg agccggatga
     3361 actgctatcc ggctaacccg ccttccgcgc aatgtcgccg ccagtggatt gctgccgtgc
     3421 gccgccagcg actgggccac cagattaccc cgcgggcgac gaaaacctgc ccataccgcc
     3481 tgactgagtt cttgagataa tccgccctca tccaccagcc acaacgcccc ggcaatctcg
     3541 ttttcgccag ccgcctgtaa aaaatgttgc cctggtgcat ccatcatccg gcgtaaatcc
     3601 agcggcgagg tccggtagtg cgcaccagac aataactgat aaacctttaa cggcgtttct
     3661 ggctcgcttc gccataacgt ttgttcaaat gcggaaatga cgatattgcc ttgtggtgta
     3721 tgggtgaagt tttcatcgtt aaaaaccagt gcctcactaa ccattttttc cagcgggcat
     3781 ccctgtgccc agcggatcgg ctgttgcagt tcaaaacggt gtaaatgcgg aaagcgagcg
     3841 caaaatttca gcaaaaaacc acgtccggta ccttcgtagc cctgcaccgt agtggttaac
     3901 aacgttcgag gaaaacgcga taccagttga tgcaacaacg gcgcaggtat ggctgcggct
     3961 tcgtcgacca ccagccagtc ggcttgctca tcgctggcta acaaggcatc cggtgcaata
     4021 aagcgaaact tctcgcccgc aaattgtgcc agtacatccg ttgccgcttt tgcgggcgcg
     4081 gtgacaatcg cactgcccgc aatacgagaa atgagttgcc ctgccaacgc cgacttaccg
     4141 cgcccacgcg cagccgttac cgctgccacg cccggcggca tggtcagtag ctgctgtaag
     4201 agttgctgtt gttctggttg tggtgcgccg gtcgcggggt gccagtcagt acggggagta
     4261 aaatgcgcca acgagaacgg ctggttttgc cgccagagga tagcgtcgtt atccgccgta
     4321 agtacgcgtt tgagatgctg gacaaaatgc ggcgtcgcaa tagggtcagg gcaatcactc
     4381 cagcgcagcg agtcggcatc aggttggttt tcccactctt cccatacagg gagtaacaaa
     4441 accagccagc ttcccgcttt caacgttccg ctaagtgctg caaaggcagc ggcatcaaag
     4501 ccgtggcggg cgtcgaatac cgcatgccgg aactcgcgcc caagtaaagt ttgtagcgcc
     4561 gagggtgaac agtggttttc agcatctagc tgcggcgaaa tccacagcca gtcgccaggt
     4621 aaggcatcac gcaacttaag agcatgatca aaacaccaac cctcttcccc gctcaacacc
     4681 agcaagcggc agatcccttc acgtttcatt tgcgctgtta atgtgtaaag cgcagtcagt
     4741 tcagccatcc ctgccccgga aattaaatgc ttttaccaaa agtattgcat tgtgccggat
     4801 cgccgctgtc gaaaccacgt ttaaaccagc tgtagcgttg ctgagaagtg ccatgtgtga
     4861 aactgtctgg tactactcgc ccctgacttt gctgttgtaa acggtcatcg ccgatggcct
     4921 gcgccgcatt cagcgcctct tccagatcgc cagtttccag aacgccttgc tgctgcatac
     4981 tatgccccca gacaccggca aaacagtcgg cctggagttc catacgcaca gataagcggt
     5041 ttacttccgc ctgcgtcgcg ttttgttgca gttgacgaac tttcggctcg atgcctaaca
     5101 gtttctgcac atgatgaccg acttcatggg cgataacgta cccctgggca aaatcgccat
     5161 ccgcgccaag tttgtctttc atgtcatcat agaaggagag atcgatataa actgtgccat
     5221 ccgccgggca atagaacggc cccataatgg actggcccgc cccgcagccg gtgcgcgtca
     5281 ttccacggta catgaccagc ttcggttgct gataggtctt acccatcttt tcgaactgtt
     5341 gtccccaggt gtcttccgtg gttgcctgaa tcaccgaggt gaattttgcg gcttcgtctt
     5401 cattagggct aattgaccgc gttgattgtt gttgagaaac cggctgcccg gtcatcaacc
     5461 cggttaaatc aacgccatag tagcctgcaa ccagcacgac tatcagtaaa atcagcccgc
     5521 ctttaccgct cggcagacgg aaaccgggac cgcccattga cggaccgcca gagctgttgc
     5581 gcctgtcttc aacattgtca ctttcacgtc gcccttgcca acgcatagat acctcaacaa
     5641 tatattcatt atagaaatga tcgtaggtgg ttcagaggaa gattaccaca ggaaagcggg
     5701 atgaaatggc ggaggaatga gcgttcaatg catatattcc aagagcggtt gtcttgccgt
     5761 gccagctgca cggcaagatg atgaacagaa aaatcagtcc agctgtacac ccaggcggcg
     5821 ggcgacggct tcataggctt cgatcaggcc accgaggctc tggcggaaac ggtctttgtc
     5881 cattttctcc agtgtttctt tgtcccacag gcggctgccg tccggggaga actcatcgcc
     5941 cagtaccact tcacctttgt acagaccaaa ctccagcttg aagtcgacca gaatcagacc
     6001 agcatcatcg aacagttttt tcagcacgtc gttcgctttg taggtcagct ctttcatacg
     6061 cgccaggttc tctttgctca cccagccaaa ggtttcgcag taagattcgt tgaccatcgg
     6121 atcgtgcatg gcatcgtttt tcaggaacag atcgaacagc ggcgggttca gctcaatacc
     6181 ttcttcgatt ccaagacgtt tcactagaga gccagcagca cggttacgca cgacacactc
     6241 aaccggcacc atatccagct ttttcaccag acattcggta tcggagagca gacgctccat
     6301 ttgagtcggg atacccgctt cagccagttt gctcataatg aagtagttga acttgttgtt
     6361 caccatacct ttgcgatcaa actgctcaat gcgcgcgcca tcccctgctg acgtatcatt
     6421 gcggaattcg agcaccaaca ggtccgggtt ttccgtgctg tatacggttt tcgctttacc
     6481 acgatacaac tcagcttgct tttgcatctt tatcactcct gggtgtgaat taacgtttta
     6541 aaatcttttg ctgtctggtg tgccggatgt tttgtcggat gcggcgtgaa cgccttatcc
     6601 gaactacgtc cgacaaacaa atttcgtgcg aattaccgct aaaatcgcgt tttcctgccg
     6661 acgcacacgt ttgcgtatca tatcagaaaa aagggccgga tgattccagc cctgtatttt
     6721 tacttgctaa acgcagcctg gaagacagct accagcgcgt cgttctgact ctgagtcaga
     6781 gtatgacctt tcggatcgat gaactgtagg ctgctgcggt tatctaaatc gccaacctgc
     6841 agtttatagt caccggatgc caggcctgga tcgctcgcgc ccagttcctg ccagtcgctg
     6901 tcagacagcg gcttataagt tacggccatg ttgccctgcg aacgggtgct gtcggtcact
     6961 ttcatgccca ctttttccag cgccgctggc agacgttgcc aaaccacatt gaacggcccg
     7021 cgtacgacca gcattggtaa accggtgtca tcagctgcac tttgtacgtc catagtggtg
     7081 gaggcacgat tttgcgcagc gttcgcggcg tcagtggcag atttatccag accggcggaa
     7141 ataacgttca tcatctccgt gctgtaacgc tgcatggaag ccgcgtctgc aaccggtttg
     7201 cccgcctgtt ccaggttcag cagtttaacc gtaaccgcct gctgataacc ctgcggctta
     7261 acagagattt gataacgacc acgatactgc tcgtcttcgt ccagacggtt ccattgtacc
     7321 caatcggtgg tcagtgtctg accagcatca tcacgttggg tgatggtgta gtttttcgcc
     7381 tgcagcacgc taaccacctg cggccacaga gtattgccac gaccattttc caccagcaat
     7441 gaagcggtat cgcccgtgaa ctgggtacgc gcgccagaaa ccagtgccaa cggctgggct
     7501 ggtggacgaa tgtccagcgc cttaccgaca gcaccactac cgttggtcac cgggattgca
     7561 taatcaccgg aggtcaccgg caaaatcatt ccagccgggg catgaagctc cgcaagcggt
     7621 gccgcttcca ggtaggcttc atcaccactg acctgacgct tatagcgtga gtcagaacta
     7681 caggcagcga gtaataaaac aagcgaaaca cccgcaacct ttgccaggcg cgacttttga
     7741 acagagtaag ccatcaaatc tccctaaact ttacagcaaa ccggcatgct taagcgccgc
     7801 tctgaccgtc tcacgaccac tgtcggtgat tggtgtcatt ggcaggcgca gcgtatcggt
     7861 cgccacaaga cccagttcct tacatgccca tttcaccggg attggattgg gttcgacaaa
     7921 tagtttgttg tgtaatggca tcagacgctg attaataacg cgtgcctcgg caaaatgccc
     7981 ttctgctgcc agtttgcaca tctgggccat atcacgcgct gcgacgttag ccgtaacgga
     8041 aataactcca tgaccgccca attgcatgaa gtccagcgcg ctcgcatcat cgccgctcag
     8101 cagaacaaaa tcatctgaaa ccagctcttt gatctggttt acacgcgtta agttccctgt
     8161 tgcctctttg attccgataa tattttttac tttcgccaga cggcccaccg tttccgggag
     8221 cagatcgcag ccagtacggg acggcacatt atacagaatt tgcggcaggt cagtatgctc
     8281 agcgatggct ttgaaatgct gatacaaacc ttcttgcgac ggacgattgt agtaaggggt
     8341 taccgtcagg cagccgacga taccactgtc attgaagcgc tgcgtcaggc taatggcttc
     8401 cgcagtagcg ttagcgccgg tcccggcaat taccggaatg cgcccatcag ccagatccag
     8461 cgtcatcatc accacatcag catgttcgtc atgatttaag gtagcggact cgccagtggt
     8521 gccaacagaa acgatcgccg aagtaccgct ggcgacatga taatcaatca gttttttcaa
     8581 gctagcccga cagacattac ctttttcatc catcggagta acaatcgcga caatacttcc
     8641 cgtgaacatg ggccatcctc tgtgcaaaca agtgtctcaa tggtacgttt ggtatggcat
     8701 taaaagcaag cagacagaac cgttctgatt gttgtatgca tgattttttt atgctttcct
     8761 taagaacaac tcacccctta aaggaataac cagtttgaca ctgtcatcgc aacattatct
     8821 ggtgatcact gcgttgggtg ccgatcgccc tggaattgtg aacaccatca cccgtcatgt
     8881 cagtagttgc ggctgtaata ttgaagacag tcgcctggcg atgctgggag aagagttcac
     8941 gtttatcatg ctgctttccg gttcatggaa tgccatcact ctgattgaat caacgttacc
     9001 gttgaaaggt gccgaactgg atcttttaat cgtgatgaag cgcacgacgg cgcgtccgcg
     9061 tccgccaatg ccagcatctg tctgggttca ggtcgatgtt gcagactccc cgcatttaat
     9121 tgaacgcttc acagcacttt tcgacgcgca tcatatgaac attgcggagc tggtgtcgcg
     9181 cacacaacct gctgaaaatg aacgggctgc gcagttgcat attcagataa ccgcccacag
     9241 ccccgcatct gcggacgcag caaatattga gcaagcgttc aaagccctat gtacagaact
     9301 caatgcacaa ggcagtatta acgtcgtcaa ttattcccaa catgatgaac aggatggagt
     9361 taagtaatga atccactgaa agccggtgat atcgcaccga aatttagctt gccggatcaa
     9421 gacggagaac aagttaattt gaccgacttc cagggacagc gtgttctggt ttatttctac
     9481 ccgaaagcca tgacccccgg ctgtaccgta caggcctgcg gcttacgcga taacatggat
     9541 gagttgaaaa aagcgggcgt tgatgtgctg ggtatcagca ccgataaacc cgaaaaactc
     9601 tcccgttttg cggaaaaaga gctgcttaac tttacgctcc tgtctgatga ggaccaccag
     9661 gtgtgcgaac aattcggcgt ctggggtgaa aagtccttca tgggcaaaac ctacgatggt
     9721 attcatcgca tcagcttcct gattgacgct gatggcaaaa tcgaacatgt ctttgacgat
     9781 ttcaaaacca gcaatcacca cgacgttgtg ctgaactggc tgaaagaaca cgcctgatta
     9841 ctttgctcca ttccgtgctg gctgcgcttg cggccagcat acctcacttc tcgtgatcaa
     9901 gatcacattc tcgctttccc ctgcgacacg ggtgtcgaat ccattttttg ctgaacgtta
     9961 atgaccatca tttttgtacc gttcagaatc cagttaatac ataacttatt gaatagattg
    10021 agttaatcag aatggcatcc tttatacaat atgaaatgca atgtttcata tcattttcaa
    10081 ggagccgaca tgaaccgctt tgtggtggcc gaaccactgt ggtgtacagg atgtaatacc
    10141 tgtctcgctg cctgttcgga cgtgcataaa acgcaaggtt tacagcaaca cccgcgcctg
    10201 gccctggcga agacgtcaac aatcactgcc cctgtcgtgt gtcatcactg tgaggaagcc
    10261 ccttgcctgc aggtctgccc ggtcaatgcc atctctcaga gggatgatgc gatccaactc
    10321 aacgaaagcc tctgtattgg ctgcaagctt tgcgccgtgg tctgcccatt tggcgcaatc
    10381 agcgcttcag gaagccgtcc ggtgaatgcc catgcgcaat atgtttttca ggctgaaggc
    10441 tcactcaaag acggcgaaga aaacgtgccg ccccaacatg cgttgctgcg ctgggaaccg
    10501 ggtgtccaga ccgtcgcggt gaaatgcgac ctgtgtgatt tcttgccaga aggtccggcc
    10561 tgcgttcgcg cttgcccgaa tcaggcgtta cggctgatca ccgacgatag cctgcaacgt
    10621 cagatgaaag aaaaacagcg ccttgccgca agctggtttg ccaatggcgg ggagtctccc
    10681 ctttccctca ctcaggagca acactaatgg atgccctgca attattaacc tggtcgctga
    10741 tcctctatct gtttgccagt ctggcttcgc tgtttttact cggtctggac agactggcta
    10801 ttaagctttc cggcatcaca tcgctggtgg gcggcgtgat tggcatcatc agcggaatta
    10861 cgcaattaca tgctggtgta actttagtcg cccattttgc cacccctttt gactttgccg
    10921 atttaaccct gcgaatggat agcctctcgg catttatggt gctagttatc tccttgctgg
    10981 tggtggtttg ttcgctctat tcattgactt atatgcgcga atacgagggc aaaggcgcgg
    11041 cggcgatggg cttctttatg aatcttttca tcgcatcgat ggttgccctg ctggtgatgg
    11101 acaacgcttt ttggttcatc gtgctgtttg aaatgatgtc gctgtcttcc tggtttctgg
    11161 tcattgccag gcaggataaa acgtcgatca acgctggcat gctctacttt tttatcgccc
    11221 acgccggatc ggtgctgatt atgatcgcct tcttgctgat ggggcgcgaa agcggcagcc
    11281 tcgattttgc cagtttcgca cgctttcact ttctccgggg ctggcgtcgg cggtgtttct
    11341 gctggccttt ttcggttttg gcgcgaaagc cgggatgatg ccgttgcaca gctggttgcc
    11401 gcgcgctcac cctgccgcac catcgcacgc ttcggcgttg atgtctggcg taatggtcaa
    11461 aatcggtatt ttcggcatcc tgaaagtagc gatggatctg ctggcgcaaa cgggtttgcc
    11521 tctgtggtgg ggcattctgg tgatggcgat cggcgcaatc tccgcgctcc tgggcgtgct
    11581 atatgcgctg gcggaacagg atatcaaacg gctgctggcc tggagtaccg tcgaaaacgt
    11641 cggcattatt ttgctggcag tcggtgtggc gatggacggt ctgtcactgc acgacccgct
    11701 gctcaccgtg gttggactgc tcggcgcact gtttcatctg ctcaaccatg cgctgttcaa
    11761 agggctgcta tttctcggcg cggacgcgat tatttcgcgt ttgcataccc acgacatgga
    11821 aaaaatgggg gcactggcga aacggatgcc gtggacagcc gcagcatgcc tgattggttg
    11881 cctcgcgata tcagccattc ctccgctgaa tggttttatc agcgaatggt acacctggca
    11941 gtcgctgttc tcactaagtc gtgtggaagc cgtagcgcta caacttgcgg gtcctattgc
    12001 tatggtaatg ctggcagtca ctggtgggct ggcagtaatg tgcttcgtca aaatgtacgg
    12061 tattactttc tgtggtgcgc cgcgcagtac acacgctgaa gaggcacagg aagtgccaaa
    12121 tacgatgatc gtcgccatgc tactgctcgc ggcactctgc gtattaattg cgcttagtgc
    12181 cagttggctg gcaccgaaga taatgcatat tgcccatgcg tttaccaata cccctcccgt
    12241 cactgtcgcc agcggaatag cacttgtacc cggcacgttt catacacggg tcactccttc
    12301 attactgttg ctgttactac tggcgatgcc tttgctgcca ggcctttact ggctgtggtg
    12361 tcgttcgcgc cgcgcagcgt ttcgtcgcac aggagatgcc tgggcatgcg gctacggctg
    12421 ggaaaacgcg atggctccgt caggcaatgg cgtgatgcag ccgctgcgtg tggtcttctc
    12481 tgcgttattt cgtctacgac aacagctcga ccctacgctg aggctaaata aaggtcttgc
    12541 gcacgtcacc gccagggctc agagcacaga acccttctgg gatgagcggg tgatccgccc
    12601 catcgtgagc gccacccaac ggctggtcaa agaaatacag catctgcaaa gcggcgactt
    12661 tcgtctctat tgcctgtatg tggtcgccgc actggttgtg ctgctaatcg ctattgccgt
    12721 ctaaggaaat caccatgaga caaactcttt gcgacggata tctggtcatt tttgcgttag
    12781 cacaggccgt gattctgctg atgctaaccc cactttttac gggtatttcc cggcagatac
    12841 gcgcgcgtat gcactcccgc cgcgggccgg ggatctggca ggattatcgc gatatccaca
    12901 aactgtttaa acgccaggaa gttgcgccga catcttcagg tctgatgttc cgcctgatgc
    12961 cgtgggtatt aatcagcagc atgctggtgc tggcgatggc cttaccacta tttattaccg
    13021 tttccccttt tgcgggcggc ggcgatctga tcacccttat ctatcttctt gccctgtttc
    13081 gttttttctt tgctctttcc gggctggata ccggaagtcc gtttgcggga gtcggtgcca
    13141 gtcgcgagtt gacgctcggc attctggtcg aaccaatgct tattctctca ctgctggtat
    13201 tggcgctgat agcaggttcc acgcatatcg agatgatcag caatacgctg gctatgggct
    13261 ggaactcgcc gctaaccacc gtactggcgt tactggcctg tggttttgcc tgcttcattg
    13321 agatgggaaa aattcccttt gatgttgctg aagcagaaca ggaattacag gaaggcccgc
    13381 tgaccgaata ttccggtgcc gggctggcgc tagcgaaatg ggggctgggg ctgaaacagg
    13441 tcgtgatggc agcactgttt gtggccctgt ttctgccctt tgggcgcgcg caagaacttt
    13501 ctctcgcctg cctgctgact tcacttgtcg ttacgctgct caaggttttg ctgatttttg
    13561 tactggcctc aatcgcagaa aacacgctgg cacgcgggcg ttttttactc attcaccatg
    13621 tgacctggct tggcttcagc cttgctgcgc ttgcatgggt cttttggtta accggtctgt
    13681 aaggagcact gacggaatat ggaaaatctt gctctgacga cgttaatgct gccttttatc
    13741 ggcgcactgg tcgtttcgtt ttcgccacaa cgtcgggccg ccgaatgggg ggttttgttc
    13801 gccgcgctga ccacgctgtg catgttgtcg ctgatctccg cgttttatca ggccgataaa
    13861 gttgccgtca cgttgacgtt ggtcaacgtg ggggatgtgg cattatttgg cctggtcatt
    13921 gatcgcgtga gtacgctgat tctgtttgtg gtggtgtttc tcggtttgct ggtcacgatc
    13981 tactccacgg gttatctgac ggataaaaat cgcgaacacc cgcataacgg cacgaatcgt
    14041 tattacgcat ttttgctggt gtttatcggc gcgatggcgg gactggtact ctcctcgacg
    14101 ctgctcggtc agttgttgtt ttttgaaatt acgggcggct gctcctgggc gttgatcagt
    14161 tattaccaga gcgataaagc gcagcgttca gcactaaaag cgttgcttat cacccatatc
    14221 ggttcgttgg ggttgtatct tgccgccgcc acgctgtttt tgcagaccgg aacgtttgtg
    14281 cttagcgcga tgagcgagtt acatggcgac gcacgttatc tggtttatgg cggcatcctg
    14341 tttgccgcgt gggggaaatc ggcccagcta ccgatgcaag cgtggctacc ggacgcaatg
    14401 gaagcgccaa caccgatcag cgcctatctc cacgccgcat cgatggtgaa agtgggcgtt
    14461 tacatttttg cccgcgccat tatcgacggc ggcaatatcc cgcatgtgat tggcggcgtt
    14521 ggcatggtca tgacactggt caccattctt tatggctttc tgatgtattt gccacagcag
    14581 gatatgaagc ggttgctggc ctggtcgacc atcactcaac ttggctggat gttcttcggc
    14641 ttgtcgctct ccatcttcgg ctcgcggctg gcgctggagg gtagcatcgc ctacatcgtc
    14701 aaccacgcgt tcgctaaaag cctgtttttc cttgtagcag gtgcgctgag ttacagctgc
    14761 ggcacgcgct tgttgccgcg tctgcgtggc gtattgcaca ccctgccgtt gccaggcgtg
    14821 ggtttctgcg tggcagcgct ggcaattacc ggtgtgccgc cgttcaacgg cttcttcagt
    14881 aaattcccgc tgtttgccgc cggttttgcg ttgtcagtgg agtactggat cctgctgccc
    14941 gccatgattc tgctgatgat tgaatcggtc gccagtttcg cctggtttat tcgctggttt
    15001 ggtcgcgtcg tgccgggcaa accgagcgag gccgtcgccg atgccgcacc gctgccaggg
    15061 tcaatgcgcc tggtgttgat tgtactgatt gtgatgtcgc tgatttccag cgtaatcgcc
    15121 gcgacctggt tgcagtaagg agatgatgaa tgaccggttc tatgatcgta aataatctgg
    15181 cgggactgat gatgctgaca tcgctgtttg tgattagcgt caaaagctat cgcctgtcat
    15241 gcggatttta cgcctgccag tcgctggtgc tggtgtctat tttcgccact ctctcgtgcc
    15301 tgttcgccac agagcaactg ctgatctggt ccgccagcgc ctttatcacc aaagtgctgc
    15361 tggtaccgtt aatcatgact tacgctgcac gaaatattcc ccagaacatc ccggaaaaag
    15421 cgttattcgg tccggcaatg atggcactgc tcgcggcgtt aattgtcctg ctttgcgcat
    15481 ttgtcgttca gcccgtgaag ctaccgatgg ctaccgggct gaaaccggcg ctggcggtag
    15541 cgttaggtca ttttctgctt ggcctgctgt gcattgtcag ccaacgcaat atcctgcggc
    15601 aaatttttgg ttactgcctg atggaaaacg gctcccatct ggtgctggcg cttcttgcct
    15661 ggcgagcacc ggaactggtg gaaataggta tcgctaccga cgccatcttc gccgtcattg
    15721 tgatggtgtt actggcaaga aaaatatggc gtacccacgg cacgctggac gtgaacaact
    15781 tgaccgcgct gaagggataa tgagatgagt tattctgtga tgttcgcttt actcctgctc
    15841 acgccgctgc ttttttcgct gctctgtttt gcctgccgga aacgggggct ctctgcgact
    15901 tgtacggtga ctgtattaca tagcttaggg atcacactgc tgctgattct agcactctgg
    15961 gtggtccaaa ctgccgctga tgcaggagaa atattcgctg cgggactgtg gcttcatatt
    16021 gatggtctgg gcggtttgtt cctcgccatt cttggtgtga ttggctttct caccggtgtt
    16081 tactcgattg gctacatgcg tcatgaagtg gcacacggcg agctttcacc cgttacactg
    16141 tgcgattact acggtttctt ccatctgttt ttgttcacca tgctgctggt tgttaccagc
    16201 aataacctga ttgtgatgtg ggcggcgatc gaagccacca ccttaagctc ggcgtttctg
    16261 gtaggcattt acggtcagcg ttcatcgctg gaatcagcat ggaagtacat cattatttgt
    16321 actgttggtg tcgcttttgg tctgttcggt accgtgctgg tatacgccaa cgccgccagc
    16381 gttatgccgc aggcagaaat ggcgatattc tggagcgagg ttcttaagca atcgtccttg
    16441 cttgacccaa cattaatgct gttggccttt gtgtttgtgc taattggctt tggtaccaaa
    16501 accgggctat ttcccatgca cgcctggctg ccggatgctc acagtgaagc gccgagtccg
    16561 gtcagcgccc tgctctccgc cgtattgctg aactgcgcgc tgttggtgct gattcgctat
    16621 tacatcatta tttgccaggc catcggcagc gatttcccca accggttgtt gctcatcttc
    16681 ggcatgttgt cggttgccgt ggcggcattt ttcattctgg tacagcggga cattaagcgt
    16741 ctgctggcgt actccagcgt ggagaacatg gggctggtcg cggtggcgct aggcattggc
    16801 gggccgctgg gaatttttgc cgcgctgctg cacaccttaa accacagtct ggcaaaaacg
    16861 ctgctgttct gcggttccgg caatgtactg ctcaagtacg gcacgcgcga tctcaacgtc
    16921 gtctgtggga tgctcaaaat catgccattt accgccgtgc tgtttggcgg cggtgcgctg
    16981 gcgctggcgc tggcgctggc agggatgccg cccttcaaca tttttcttag cgaatttatg
    17041 accgttaccg caggactggc acgtaatcac ctgctgatta tcgtcctgct gttattgctg
    17101 ttaacgctgg tgctggcggg cctggtacgg atggctgcgc gggtgttaat ggcgaaaccg
    17161 ccgcaggccg ttaaccgggg tgatctcggc tggttgacca cctcgccaat ggtgattctg
    17221 ctggtcatga tgctggcgat gggaacgcat attccacaac ctgtcatcag gatcctggcg
    17281 ggcgcttcca ctatagtcct ctcagggacg cacgacctgc ctgcacaacg tagcacctgg
    17341 catgattttt tgccttcagg caccgcatct gtttcggaga aacacagtga acgttaattc
    17401 atcgtcaaat cgtggcgaag cgattctcgc cgccctgaaa acgcagttcc ccggcgcggt
    17461 gctggatgaa gagcgacaaa cgcctgaaca ggtcaccatt acggtgaaaa tcaatctgct
    17521 gcctgacgtt gtacattatc tttattatca acatgatggc tggcttccag tcctgtttgg
    17581 caacgacgag cggacactta acggtcatta cgcggtttat tatgcccttt caatggaagg
    17641 ggccgaaaaa tgctggatcg tggtgaaggc actggtcgat gccgacagtc gggagtttcc
    17701 gtcagtcaca ccgcgcgtcc ctgccgcggt ctggggcgag cgagaaattc gcgatatgta
    17761 cgggctgatt ccggttggcc tgccggatca gcgtcgcctg gtgttgcccg atgactggcc
    17821 ggaagatatg catccgctgc gcaaagatgc gatggattat cgactgcgcc ctgaaccgac
    17881 gacagattcc gaaacgtatg cgtttatcaa tgagggcaac agcgatgcgc gggtgatccc
    17941 tgtcggcccg ctgcatatca cctcctatga accgggtcac ttccgcttgt ttgtggatgg
    18001 cgagcaaatt gtcgatgctg attaccgcct gttttatgtc catcgcggca tggagaaact
    18061 ggcagaaacg cggatgggct acaacgaagt gaccttctta tcggaccgcg tgtgtgggat
    18121 ttgcggtttt gcccacagtg tggcctatac caactcggtt gaaaatgcac tggggattga
    18181 ggtgccgcaa cgagcacata ctattcgctc gattctgctg gaagtcgagc ggctacacag
    18241 tcatttgctc aaccttggcc tctcctgcca ttttgttggt tttgataccg gctttatgca
    18301 atttttccgc gtgcgggaaa agtcgatgac gatggcggaa ttgctgaccg ggtcgcgtaa
    18361 aacctacggt ctgaatctga ttggtggtgt tcgccgcgat attctcaaag agcaacgtct
    18421 gcaaacgctg aaactggtgc gcgagatgcg cgccgacgtg tcggagctgg tagagatgct
    18481 gcttgctacg ccgaatatgg aacaacgcac tcagggcatt ggcattctcg accgacaaat
    18541 cgcccgtgat tatagccctg tagggccgct gatccgcgca gtggttttgc ccgtgatttg
    18601 cgctttgatc acccctacgc cgactacggc aatattccaa aaacactgtt tacctttacc
    18661 ggcggcgatg ttttctcccg cgtgatggtc cgtgtcaaag agacgtttga ttcgctggca
    18721 atgctggaat ttgccctcga caacatgccg gataccccac tgctgaccga aggctttagc
    18781 tataaacctc acgcattcgc gctgggcttt gttgaagcgc cacgcggtga agacgtgcac
    18841 tggagcatgc tcggtgataa ccaaaaattg ttccgctggc gctgtcgtgc cgccacctac
    18901 gccaactggc cggtgttgcg ttacatgctg cgcggcaata ccgtttctga cgcaccgctg
    18961 attatcggta gccttgatcc ctgctactcc tgtaccgacc gtgtgacgct ggtagatgtg
    19021 cgcaagcgcc agtcaaaaac cgtgctgtat aaagagatcg aacgctacgg cattgatcgt
    19081 aaccgttcgc cgctgaagta aggacagaag atgctgaagt tactgaaaac tattatgcgc
    19141 gccggaaccg cgacggtgaa atatcccttc gcgccactgg aggtcagccc aggctttcgc
    19201 gggaaaccgg acctgatgcc cagccaatgt attgcctgcg gtgcctgcgc ctgtgcttgt
    19261 ccggcaaatg cgctgactat ccagaccgac gaccagcaaa attcgcgcac ctggcagctc
    19321 tatctggggc gttgtattta ctgcggacgt tgtgaagaag tgtgcccgac cagagccatc
    19381 cagcttacca ataactttga actgaccgtc acccataaag ccgatctcta tacccacgcg
    19441 acgttccatc tacaacgttg cagccgttgc gaacgcccgt ttgccccgca aaaaaccgtc
    19501 gcactggctg ctgaattgtt ggcacagcaa caaaatgctc cacaaaaccg cgaaatgtta
    19561 tgggcgcaag cgagcgtctg cccggagtgc aaacaacgcg caacgctgat caacgacgat
    19621 acagatgtac cgctggtggc taaggagcag ctatgagtcc agtgcttacg caatatgtca
    19681 gccagcccat cacgctggac gagcaaacgc aaaagatgaa gcggcatttg ctacaggata
    19741 tccgtcgctc ggcttacgtt tatcgcatcg attgcggcgg ctgcaacgcc tgtgaaatcg
    19801 aaatttttgc tgccatcacc cccgtattcg acgcagaacg ttttggcatt aaggttgttt
    19861 catcaccgcg tcacgccgat attttgttat ttactggcgc agtcacccgg gcgatgcgta
    19921 tgcctgcact tcgggcgtat gagtctgccc ccgatcataa aatctgtgtt tcctacggcg
    19981 cgtgcggtgt cggcggcggt attttccacg atctctacag cgtctggggc ggcagcgaca
    20041 ccattgtccc cattgatgtt tggatccccg gctgcccgcc aacaccggcc gccaccattc
    20101 acggtttcgc cgtggcgctc ggtttgctgc aacagaagat tcacgcggtg gattatcgcg
    20161 atcccaccgg ggtgactatg caaccgttgt ggccgcagat cccgccatca cagcgtatcg
    20221 ccattgagcg agaagcgcgg cggctggcgg gctatcgtca ggggcgagaa atttgcgatc
    20281 ggctcctgcg ccatttaagc gacgatccta caggaaatcg ggttaacacc tggttgcgcg
    20341 atgccgacga tccacgtctc aatagtatcg ttcagcaact ctttcgcgta ctccgggggt
    20401 tacatgactg aagagtgcgg ggaaattgtt ttctggacgc tgcgaaaaaa gtttgtcgcc
    20461 agtagcgacg agatgccgga acacagctct caggtaatgt attactcgct ggctatcggt
    20521 catcacgttg gcgtgattga ttgtctgaat gtcgccttcc gctgcccact gacggaatac
    20581 gaagattggc ttgcactggt cgaagaggag caagcccgac gtaagatgct gggggtgatg
    20641 acttttggtg agattgttat tgacgccagc cacaccgccc tgttgacccg ggcattcgcg
    20701 ccactggcag atgacgcgac gtctgtgtgg caagcgcgta gcattcaatt cattcatctg
    20761 ttggatgaaa ttgtgctgga accggctatc tatctgatgg ccagaaaaat tgcgtgagaa
    20821 ggatttctca ttaataagga ctgttgatgg ctatgtcaga cgaagcgatg tttgccccgc
    20881 cgcaaggaat aacaattgaa gcggtaaacg gaatgctcgc ggagcggtta gcgcagaaac
    20941 acggcaaggc gtctttatta cgcgccttca tcccgctgcc gccgccgttc agcccggtac
    21001 aacttattga actgcatgtt ctcaaaagca acttctatta ccgctaccat gatgatggca
    21061 gcgatgtgac ggcaacaaca gagtatcagg gcgagatggt cgattattcg cgttacgccg
    21121 tccttctcgg cagtagtgga atggcggagc tacgctttat tcgtacccac ggcagccgtt
    21181 ttactcccca ggattgcaca ctgtttaact ggctggcacg cattatcacc ccggttctgc
    21241 aatcatggct caatgatgaa gcacagcagg tggcgctgcg tttgctggag aaagatcgcg
    21301 atcatcatcg ggtactggtg gatatcacta atgcagtgct gtcacatctt gatctcgacg
    21361 atctgatcgc tgacgtcgct cgtgagatcc atcatttttt cggtctggct tcagtcagta
    21421 tggtactggg cgatcatcga aagaacgaga agttcagcct gtggtgcagc gatctttctg
    21481 cctcacattg tgcgtgtctg ccacacaata tgcctggcga cagtgtattg ctgacacaaa
    21541 cgctacaaac ccgacaaccg accttgacgc accgtgcaga tgatctgttt ctctggcaac
    21601 gcgacccgtt attactctta cttgcatcta acggctgcga atctgcgctc cttataccgc
    21661 ttacctttgg caaccataca ccgggtgcat tgttgctggc gcatacctct tccactctct
    21721 ttagtgagga aaactgccag ctactacaac acatagccga tcgcatcgct attgccgttg
    21781 gcaatgccga tgcctgccgt agaatgaccg atttgcagga aagtttgcag caagaaaacc
    21841 accagcttag cgagcagctc ctttcgaatc tgggcgtcgg tgacattatc tatcaaagcc
    21901 aggcaatgga agacctgctc cagcaggtag atattgtggc gaagagcgac agtacggtgt
    21961 tgatttgcgg tgaaaccgga accggcaaag aggtgatcgc cagagcgatc catcaactta
    22021 gcccgcgaca cgacaagccg ctggtcaaaa tcaactgcgc tgccatcccc gccagtcttc
    22081 tggaaagtga gttattcggt catgacaaag gggcgtttac tggtgcgatt aatacccatc
    22141 gtggtcgttt tgaaattgcc gatggcggca cgttgtttct cgatgaaatt ggcgatctgc
    22201 cgttagaact tcagcctaaa ctgctgcgcg tattgcagga acgggagatt gagcgtctcg
    22261 gcgggagtag aacgatcccg gtaaatgtca gagtcattgc cgccaccaac cgtgatttgt
    22321 ggcaaatggt tgaagatcgc cagtttcgca gcgatctctt ttatcgcctg aatgtcttcc
    22381 cactggaatt gcctccgctg cgcgaccgtc cggaagatat ccctctttta gcaaagcatt
    22441 tcacgcaaaa aatggcgcgc catatgaatc gttcaattga cgccatcccg accgaggcgc
    22501 tacgccagtt gatgtcgtgg gactggccgg gcaacgtgcg cgagctggaa aacgtgattg
    22561 agcgagcggt actgctgact cgcggtaaca gtctgaattt acatcttaat gtccgacaaa
    22621 gccgtttact gccgacgcta aatgaagatt cagcgcttcg cagttcaatg gcgtagttgc
    22681 tgcacccgac gacgccagag aatgacgaag aagaacgtca gcgcattgtt caggtattgc
    22741 gagaaaccaa tggcattgtt gccgggcccc gtggcgcggc gacacgatta gggatgaagc
    22801 gcaccacgct gctgtcacga atgcagcgtc tgggaatctc ggttcgcgag gtgttgtaat
    22861 ctgcttttgc aggagtatgc atgagaaaca aactctcttt cgacttgcag ttgagcgcca
    22921 gaaaagcggc aatcgctgaa cggattgccg cccataaaat tgcccgcagt aaagtgtcgg
    22981 tctttttaat ggcgatgtcc gctggcgtgt ttatggcgat gtccgctggc gtgtttatgg
    23041 cgatcggatt tactttttac ctttccgtta tcgccgatgc cccgtcttca taggcattaa
    23101 cccatctggt gggcggcctt tgctttacac ttggctttat tttgctggcg gtttgcggca
    23161 ccagcctgtt cacctcgtcg gtaatgacgg taatggcaaa aagtcggggc gttattagtt
    23221 ggcgaacttg gctgattaac gcacttctgg tggcctgcgg taatctggca ggtattgcct
    23281 gtttcagttt gttaatctgg ttttccgggc tggtgatgag tgaaaacgcg atgtggggag
    23341 tcgcgatttt acactgcgcc gagggcaaaa tgcatcatac atttactgaa tctgtcagcc
    23401 tcggcattat gtgcaatctg atggtttgcc tggcgctgtg gatgagttat tgcggacgtt
    23461 cgttatgcga caaaatcgtc gccatgattt tgtccatcac cctgtttgtc gccagtggct
    23521 ttgagcactg tatcgccaat ttgtttgtga ttccgttcgc cattgccatt cgccatttcg
    23581 cccctacctc cttctggcaa ctggcgcaca gtagcgcaga caattttccg gcactgacgg
    23641 tcagccattt tattaccgcc aatctgctcc cggtgatgct gggtaatatt atcggcggtg
    23701 cagtgctggt gagtatttgt tatcgggcta tttatttacg tcaggaatcc tgaaaaatca
    23761 gcccggcgaa acagttcgtc gggctattta tttacgtcag gaatcctgaa aaatcagccc
    23821 ggcgaaacag ttcgtcgggc tgaagattat tcttgcgcga tttgcccatc gggccaggcg
    23881 tggatcacgg ctttgatcag cgtcgccaat ggaatggcga agaatacgcc ccagaatccc
    23941 cacaaaccac cgaagatcac caccgataaa ataatcacca gcggatgcag gttaaccgct
    24001 tcggagaaca acaccggtac tagcaggttg ccgtccagcg cctgaataat cagatacacc
    24061 gcgaagcagc tccagaattc agtacctgcg ccaaactgga atagcgccac gccaaccacc
    24121 ggaatggtca ccacaaatgc gccaatgtac ggaataagaa ccgagaagcc gaccagcact
    24181 gccagcagca gcgaatagtt cagcccgaag agcaagaacc ccagccaggt ggcgatcccc
    24241 accacgatca tctccagcac tttgccgcgg atatagttgg tgatttgttg attcatctcc
    24301 ttccacacct gtcctgccag cccacggtta cgcggcagca cccggcgaac ggcgttcagc
    24361 atctgctctt tgtctttcag caggaagaag accatcaatg gcaccagcac cagatagacg
    24421 gctatggtca gcaaaccgac cagcgaggcg agggaaattt tcaccaccga atcgcccatg
    24481 gtcagcatcc gactgcgcat attttcggcc attgcatcaa taatgcccgc gtccattaac
    24541 accggatagc ggcgcggcaa cgtggcggca aagtcagaaa gcttattgag catccccggc
    24601 atatcgcgga ttaagtagat gccctgttgc caggcgatag gcagtaccac gaacgccatc
    24661 agtagcaata taccgacaaa aaccaccaat acaatcgacg tcgcccagcg gcgggagcag
    24721 ccaatagatt gcaggcgcac ggttggccat tccagcaaat aagccagcac aatagccacc
    24781 agcaacggag caagcaggcc actaaagaaa aagataatgc caaatccggc aactaaaata
    24841 accagcaagg caatcgcttc cgggtcgcta aaacggcggc gataccattg catcaacatt
    24901 tcgagcataa agaccttccc tgaacctcaa gagcgggatt gcgatccgca attgtatcga
    24961 aatgtcacaa aaaagacttc gctttttatg acggattcag gaaactgaaa agtcatttga
    25021 gtgggctaat cttcgccgtt acactcaaag gcggcgaggt gggaacgata tttcacagta
    25081 tcggtcaaat gactatcttc agaaatacag gatagaggtt atgttcaggc agttgaaaaa
    25141 aaacctggtt gcaaccctca ttgctgctat gaccattggt caggtagccc cggcgtttgc
    25201 cgacagcgca gacaccttgc cggatatggg aacctccgca ggaagcacgc tttccattgg
    25261 tcaggaaatg cagatgggcg actattatgt ccgccagcta cgcggcagcg cgccgttaat
    25321 taatgacccg ctgttaacgc aatatattaa ttcgctgggg atgcgtctgg tttcgcatgc
    25381 caattcggtt aagacaccgt ttcatttttt tctgatcaac aacgacgaaa ttaacgcctt
    25441 tgctttcttt ggcggcaacg tggtgctgca ctctgccctg ttccgttatt ccgataacga
    25501 aagtcaactg gcttcagtta tggcgcacga aatctcccac gtcacccaac gtcacctggc
    25561 gcgagcgatg gaagatcaac aacgcagcgc gccgctgacc tgggtcggcg cgttaggttc
    25621 tattttactg gcgatggcca gtccgcaggc ggggatggcg gcgctgaccg gtacactggc
    25681 gggaacgcgc caggggatga tcagtttcac ccagcaaaat gaacaggaag cggaccgcat
    25741 tggtattcag gtgctgcaac gctcgggatt cgatccacag gcgatgccga ccttcctgga
    25801 aaaattgctc gatcaggcgc gttactcctc gcgcccgccg gaaattttat tgactcaccc
    25861 gttgccggaa agtcgtctgg cagatgcccg caaccgtgct aatcagatgc gcccgatggt
    25921 ggtgcagtcg tcggaagatt tctatctggc gaaagcgcgc acactgggga tgtataattc
    25981 cggacgtaac cagctcacca gtgatttgct ggatgaatgg gcgaaaggaa acgttcgtca
    26041 gcaacgagcg gcgcaatatg gtcgtgcttt acaggcgatg gaagccaata aatacgacga
    26101 ggcgcgtaaa acgctgcaac cgttactggc ggcagaacct ggcaacgcat ggtatctcga
    26161 tctggctacc gatatcgatc ttgggcaaaa caaagccaat gaggcaatca atcgcctgaa
    26221 aaatgcccgc gatttacgca ccaatccggt gttgcagctc aacctggcga acgcttatct
    26281 gcaaggcggt caaccacaag aagcggccaa tattctgaat cgctacacct ttaataataa
    26341 agatgacagc aacggctggg atttgctggc acaggcggaa gccgcgctaa ataaccgcga
    26401 tcaggagctg gctgcgcgag cagaaggtta tgcgctcgcc ggacgactcg atcaggccat
    26461 ttcgctgttg agtagcgcca gttcgcaggt gaaattaggc agcctgcaac aagcgcgtta
    26521 cgatgcgcgc atcgaccagt tgcgccagct gcagaaacgc tttaagcctt ataccaagat
    26581 gtaattcgca ttaaaggaag aacaatgacc aaacaggtaa aaatctacca taacccacgc
    26641 tgttcaaaga gccgggaaac gctgaacctg ctgaaagaaa acggcgtgga gccagaagtg
    26701 gtgctctacc ttgagacacc cgccgatgcg gcaacgctgc gcgatttgct gaaaatgctg
    26761 gggatgaata gcgcccgtga attgatgcgc cagaaagagg atctttataa agaactaaac
    26821 ctggctgaca gttcgcttag cgaagaggcg ctgattcagg caatggtgga taatccgaag
    26881 ctgatggagc gcccgattat ggtcgcgaat ggcaaagcgc ggattggtcg tccaccggag
    26941 caggtactgg aaatcgtggg ttaagatgtg ctccatcgct agttgaagca cattgccgga
    27001 tgcgacgctt gccacgtctt atccggccta cgaatactat ggattaactt cgcgtaggtc
    27061 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
    27121 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
    27181 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
    27241 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
    27301 agaaagtggc tgcaatttgt agatctgccc ccagtcgaga cgcgacgcga gatccggtaa
    27361 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga
    27421 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
    27481 acactcaatg ttgtcgatac agaccagcga caaatgctcc ataccgtcga gcacttccgg
    27541 aacaaaccag gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
    27601 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
    27661 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
    27721 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
    27781 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
    27841 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
    27901 ctgttatatc cgcgtcttct gcgtccagca ccacttcttc agggcgcagc acactgatca
    27961 gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact
    28021 ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca
    28081 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg
    28141 aagcaccaat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
    28201 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
    28261 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
    28321 aagtggtatt tggcgtagag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa
    28381 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
    28441 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
    28501 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag tgcaaaccag tgagcattaa
    28561 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtacccc accagcacgc
    28621 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
    28681 ccgccagggt ggtaatagag atgatgatgg ttttggagtt tggcgtttgc ccttcagccg
    28741 ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
    28801 ttgcgcccat tgccgcaggt ggaaacagca cgtccaacca gccggtcccc gctttcttca
    28861 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
    28921 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
    28981 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
    29041 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
    29101 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
    29161 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
    29221 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
    29281 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
    29341 tggtcaatcg atgcagtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
    29401 ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
    29461 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
    29521 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
    29581 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg
    29641 tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc
    29701 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
    29761 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
    29821 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
    29881 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc gacggattat
    29941 acctcctttc ttcagggcgg caatattatt ttcgttgact ttagtcaaaa tgataacagt
    30001 ttgagataaa gttattttat attcaaatgg ttatgaaaga agattattcc atccgaaaac
    30061 taacctttgc cctggcacaa gtcttctttc gccgcgcgcc cggggaaaag acgtgcaaaa
    30121 aggttgtgta aaacagtctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt
    30181 atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag
    30241 ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg
    30301 cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcgct
    30361 gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg
    30421 taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta ttgatctggt
    30481 cgccatgtgc gttaatgacc tggtggtgca aggtgcggaa ccgctgtttt tcctcgacta
    30541 ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg gcatcgcgga
    30601 aggttgtctg caatcgggct gttctctggt gggtggcgaa acggcagaaa tgccggggat
    30661 gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga
    30721 aatcatcgac ggctctaaag tcagcgacgg cgatgtgctg attgcactcg gttccagcgg
    30781 tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc
    30841 gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat
    30901 ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct
    30961 gaccggcggc ggcttctggg aaaacattcc gcgcgtattg ccagataata cccaggcagt
    31021 gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa
    31081 cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga ttatcgccct
    31141 gcctgctccg gaagtggaca aagccctcgc cctgctaaat gccaacggtg aaaacgcgtg
    31201 gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat
    31261 attgtggtgc ttatttccgg caacggaagt aatttacagg caattattga cgcctgtaaa
    31321 accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc
    31381 cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac
    31441 agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg
    31501 gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg
    31561 cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt
    31621 caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa
    31681 ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa
    31741 gatgacatca ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc
    31801 tggtttgccg atggtcgtct gaaaatgcac gaaaacgccg cgtggctgga tggtcaacgt
    31861 ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct
    31921 gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt
    31981 ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt
    32041 gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc
    32101 agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg
    32161 attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc
    32221 cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc caacgctcat
    32281 tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac
    32341 ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa
    32401 cgccagctct ccgtcaatca acaaaactgg ctgcgtcatt attttaagca gtatctgcgt
    32461 cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaagat
    32521 gattacacct atctggcggt ggaaattatc cgtagcgata gcatccgtta cgcgctgctg
    32581 gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga
    32641 cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa
    32701 ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa
    32761 tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag
    32821 cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg
    32881 gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt
    32941 cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg
    33001 aacaaaccac tgccgcgttt acgccatatt tggtttgata aagcccagtt ccgcaatggt
    33061 tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat
    33121 gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac
    33181 atttaccgcg tggcgaaaga ttcacgcatc atcgactcaa tgatccacgc cgcacataac
    33241 ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt
    33301 cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg
    33361 aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac
    33421 gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg
    33481 ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa
    33541 aacccatacc gcccggtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt
    33601 ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt
    33661 atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc
    33721 tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat
    33781 ctggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat
    33841 gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac
    33901 tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcgc
    33961 ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt
    34021 tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg
    34081 gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc
    34141 aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag
    34201 ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa
    34261 acagcgggtg catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat
    34321 gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc
    34381 cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct
    34441 gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc
    34501 ccgtctgatt tttatgggcg tggaacatac ccaaccggaa aaaggtcgca aactggttat
    34561 tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac ctattctcgt
    34621 tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tattttcctg gcggggtcat
    34681 caataaagag aattttcagc gcgctcgcat ggcggcagca caaaaactgg aaactttaac
    34741 ctggcaattc cgtattcagg gctggaacgt tgcaatgggc gcttccggta ccataaaagc
    34801 cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct
    34861 ggaaaaactg gtaaaagaaa ttttacgtca ccgtaatttc gcatcgctga gtttaccggg
    34921 tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat gtggtgtgtt
    34981 tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact
    35041 gtatgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct
    35101 cgccaaccag tatcacatag acagcgaaca ggcccgacga gtgctggata ccactatgca
    35161 aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct
    35221 actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca
    35281 tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca
    35341 gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg attaagctcg acgatctgcc
    35401 gcgctttacc ttgtttaaga agaaacagtt cctgccactg atacagctat tgcgccttgg
    35461 cgtattacta aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac
    35521 tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgctggt
    35581 actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa
    35641 aattgaagaa gaaagtaccc cagaaatcgc cgcttaatgc tttgcgggcc gacgagaaat
    35701 gtcggcccgc attattcagg cactttcgcg aatgggttcg atttcattca gcgtatcaat
    35761 taacggttgc ggcttaccaa taagataacc ctgcatataa tcgatcccca aagagagcac
    35821 cgcctcgcgg atctcttcgt tttcaacgta ctctgccact acccgcattt tcttcattcg
    35881 cgccagatgg caaatcgacg ccacaatctg ataatccaga ctatttgaca caatattgcg
    35941 gataaagctg ccgtcaattt taagcagatc ggcattcaca tttttaagcc gtgcatagct
    36001 ggcgtagccg gtgccgaaat catcaatcgc aatctggcag cctaattcct gaagatgctg
    36061 caaggtgatt tgcgcctgct taacattggt cagagcatta ctttcggtga cttcaaaaat
    36121 aagttgccac gcttcaatct gatatttagc cagcaactga ctgacttcaa caggaaaacg
    36181 agcctgacat accgaggttg gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc
    36241 tctgttttca gccataaatt gcagcgtatg ctcaatgacc cacatgtcga tactcgacga
    36301 taaaccaaat tcgtgcgcga ccggcaagaa gctaccgggg ccgatcagtt catcattctc
    36361 acctttcatg cgcagaagaa tttcatggta aacatcacca cgcataccgg taatcggctg
    36421 ggccatcagg aaaaaatggt tgtgttccag cgcctgctgt agtcgattca tcatcgcgac
    36481 tttatctttc aattcgcgtt gcaaatacat tgccccgcaa cgctgcatat tttccggggc
    36541 gttggtcacg atggaaagtt cggcgaccgt atttagctct cccagcagca ggtagatatg
    36601 attcactggc gagcgcacat agcagtaact gacgccaatc tgcggttgca tcggcatccc
    36661 atcccaaaag aaacgaaatt gcttgagatg actatccagt gcggtaatgc gctcctggtg
    36721 cgattctgta ttcagtcgca gcgcgagatc gttacccgaa agctgataaa catcttcacc
    36781 cggttccagc aagggtgaca gccagtgaga aagtttttgc tt
//
LOCUS       DAZPDE010000022        36587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPDE010000022 DAZPDE010000000
VERSION     DAZPDE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.022"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHU0J_001031"
     CDS             <1..190
                     /locus_tag="ACHU0J_001031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215814.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHU0J_001032"
     CDS             complement(156..1391)
                     /locus_tag="ACHU0J_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1215815.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHU0J_001033"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHU0J_001033"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHU0J_001034"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHU0J_001034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHU0J_001035"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHU0J_001035"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHU0J_001036"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHU0J_001036"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHU0J_001037"
     CDS             complement(2077..3462)
                     /locus_tag="ACHU0J_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1215816.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHU0J_001038"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHU0J_001038"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1215817.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHU0J_001039"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHU0J_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1215818.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHU0J_001040"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHU0J_001040"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1215819.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHU0J_001041"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHU0J_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1215820.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHU0J_001042"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHU0J_001042"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1215821.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHU0J_001043"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHU0J_001043"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1215822.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHU0J_001044"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHU0J_001044"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1215823.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHU0J_001045"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHU0J_001045"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1215824.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHU0J_001046"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHU0J_001046"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1215825.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHU0J_001047"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHU0J_001047"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1215826.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHU0J_001048"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHU0J_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1215827.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHU0J_001049"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHU0J_001049"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1215828.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHU0J_001050"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHU0J_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1215829.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHU0J_001051"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHU0J_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1215830.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHU0J_001052"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHU0J_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1215831.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHU0J_001053"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHU0J_001053"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1215832.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHU0J_001054"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHU0J_001054"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1215833.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHU0J_001055"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHU0J_001055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1215834.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHU0J_001056"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHU0J_001056"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1215835.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHU0J_001057"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHU0J_001057"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1215836.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHU0J_001058"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHU0J_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1215837.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHU0J_001059"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHU0J_001059"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1215838.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHU0J_001060"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHU0J_001060"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1215839.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHU0J_001061"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHU0J_001061"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1215840.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHU0J_001062"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHU0J_001062"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1215841.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHU0J_001063"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHU0J_001063"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1215842.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHU0J_001064"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHU0J_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1215843.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHU0J_001065"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHU0J_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1215844.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACHU0J_001066"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACHU0J_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHU0J_001067"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACHU0J_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1215845.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHU0J_001068"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACHU0J_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1215846.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36398..36473)
                     /locus_tag="ACHU0J_001069"
     tRNA            complement(36398..36473)
                     /locus_tag="ACHU0J_001069"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACHU0J_001070"
     tRNA            complement(36482..36558)
                     /locus_tag="ACHU0J_001070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8939 a        10012 c         9217 g         8419 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gatgtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZPDE010000023        34996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPDE010000023 DAZPDE010000000
VERSION     DAZPDE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.023"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHU0J_001071"
     CDS             complement(<1..815)
                     /locus_tag="ACHU0J_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1215847.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHU0J_001072"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHU0J_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHU0J_001073"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHU0J_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACHU0J_001074"
     CDS             complement(1398..1874)
                     /locus_tag="ACHU0J_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1215848.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHU0J_001075"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACHU0J_001075"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1215849.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACHU0J_001076"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACHU0J_001076"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1215850.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACHU0J_001077"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACHU0J_001077"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1215851.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACHU0J_001078"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACHU0J_001078"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1215852.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACHU0J_001079"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACHU0J_001079"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1215853.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHU0J_001080"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="ACHU0J_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1215854.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHU0J_001081"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACHU0J_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1215855.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="ACHU0J_001082"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="ACHU0J_001082"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1215856.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="ACHU0J_001083"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="ACHU0J_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1215857.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="ACHU0J_001084"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="ACHU0J_001084"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1215858.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="ACHU0J_001085"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="ACHU0J_001085"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1215859.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="ACHU0J_001086"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="ACHU0J_001086"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1215860.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="ACHU0J_001087"
     CDS             13849..14553
                     /locus_tag="ACHU0J_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1215861.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="ACHU0J_001088"
     CDS             14758..15471
                     /locus_tag="ACHU0J_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1215862.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="ACHU0J_001089"
     CDS             complement(15507..16463)
                     /locus_tag="ACHU0J_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR1215863.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHU0J_001090"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACHU0J_001090"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1215864.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="ACHU0J_001091"
     CDS             complement(17701..18462)
                     /locus_tag="ACHU0J_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1215865.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="ACHU0J_001092"
     CDS             18595..19005
                     /locus_tag="ACHU0J_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1215866.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="ACHU0J_001093"
     CDS             complement(18967..20073)
                     /locus_tag="ACHU0J_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1215867.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="ACHU0J_001094"
     CDS             complement(20084..21217)
                     /locus_tag="ACHU0J_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1215868.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="ACHU0J_001095"
     CDS             complement(21210..22946)
                     /locus_tag="ACHU0J_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1215869.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHU0J_001096"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACHU0J_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1215870.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHU0J_001097"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACHU0J_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1215871.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHU0J_001098"
     CDS             24837..26057
                     /gene="rhlE"
                     /locus_tag="ACHU0J_001098"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1215872.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26051..26149)
                     /locus_tag="ACHU0J_001099"
                     /pseudo
     CDS             complement(26051..26149)
                     /locus_tag="ACHU0J_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHU0J_001100"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACHU0J_001100"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1215873.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="ACHU0J_001101"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="ACHU0J_001101"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1215874.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHU0J_001102"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="ACHU0J_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1215875.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHU0J_001103"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="ACHU0J_001103"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1215876.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHU0J_001104"
     CDS             31266..31325
                     /gene="ybiE"
                     /locus_tag="ACHU0J_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1215877.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHU0J_001105"
     CDS             complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACHU0J_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1215878.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHU0J_001106"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACHU0J_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1215879.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHU0J_001107"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACHU0J_001107"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1215880.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34996)
                     /locus_tag="ACHU0J_001108"
                     /pseudo
     CDS             complement(33012..34996)
                     /locus_tag="ACHU0J_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8294 a         8971 c         9454 g         8277 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gtgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcgtcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggg ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgc
//
LOCUS       DAZPDE010000024        34800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPDE010000024 DAZPDE010000000
VERSION     DAZPDE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.024"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHU0J_001109"
     CDS             <1..2098
                     /locus_tag="ACHU0J_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1215881.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHU0J_001110"
     CDS             2095..3183
                     /locus_tag="ACHU0J_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1215882.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHU0J_001111"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHU0J_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR1215883.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHU0J_001112"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHU0J_001112"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1215884.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHU0J_001113"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHU0J_001113"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1215885.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHU0J_001114"
     CDS             8051..8602
                     /locus_tag="ACHU0J_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1215886.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHU0J_001115"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHU0J_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1215887.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHU0J_001116"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHU0J_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1215888.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHU0J_001117"
     CDS             complement(11683..12681)
                     /locus_tag="ACHU0J_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1215889.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHU0J_001118"
     CDS             complement(12760..13452)
                     /locus_tag="ACHU0J_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1215890.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHU0J_001119"
     CDS             complement(13529..14032)
                     /locus_tag="ACHU0J_001119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1215891.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHU0J_001120"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHU0J_001120"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1215892.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHU0J_001121"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHU0J_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1215893.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHU0J_001122"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHU0J_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1215894.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHU0J_001123"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHU0J_001123"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1215895.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHU0J_001124"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHU0J_001124"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1215896.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHU0J_001125"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHU0J_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHU0J_001126"
     CDS             complement(22127..23560)
                     /locus_tag="ACHU0J_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1215897.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHU0J_001127"
     CDS             complement(23623..24072)
                     /locus_tag="ACHU0J_001127"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1215898.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHU0J_001128"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHU0J_001128"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR1215899.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHU0J_001129"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHU0J_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1215900.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHU0J_001130"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHU0J_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHU0J_001131"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHU0J_001131"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1215901.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHU0J_001132"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHU0J_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHU0J_001133"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACHU0J_001133"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1215902.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHU0J_001134"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACHU0J_001134"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(34082..34157)
                     /locus_tag="ACHU0J_001135"
     tRNA            complement(34082..34157)
                     /locus_tag="ACHU0J_001135"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="ACHU0J_001136"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="ACHU0J_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8488 a         8948 c         9143 g         8221 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttcctgcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgtcaccg
//
LOCUS       DAZPDE010000025        34675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPDE010000025 DAZPDE010000000
VERSION     DAZPDE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.025"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="ACHU0J_001137"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="ACHU0J_001137"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1215903.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="ACHU0J_001138"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="ACHU0J_001138"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1215904.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="ACHU0J_001139"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="ACHU0J_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1215905.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="ACHU0J_001140"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="ACHU0J_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1215906.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="ACHU0J_001141"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="ACHU0J_001141"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1215907.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="ACHU0J_001142"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="ACHU0J_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1215908.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15106)
                     /locus_tag="ACHU0J_001143"
     CDS             complement(14756..15106)
                     /locus_tag="ACHU0J_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1215909.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15091..15498)
                     /locus_tag="ACHU0J_001144"
     CDS             complement(15091..15498)
                     /locus_tag="ACHU0J_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1215910.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15495..16058)
                     /locus_tag="ACHU0J_001145"
     CDS             complement(15495..16058)
                     /locus_tag="ACHU0J_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1215911.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16049..16519)
                     /locus_tag="ACHU0J_001146"
                     /pseudo
     CDS             complement(16049..16519)
                     /locus_tag="ACHU0J_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="ACHU0J_001147"
     CDS             complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="ACHU0J_001147"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1215912.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="ACHU0J_001148"
     CDS             complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="ACHU0J_001148"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1215913.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="ACHU0J_001149"
     CDS             complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="ACHU0J_001149"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1215914.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="ACHU0J_001150"
     CDS             complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="ACHU0J_001150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1215915.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21672..21818)
                     /gene="ygdT"
                     /locus_tag="ACHU0J_001151"
     CDS             complement(21672..21818)
                     /gene="ygdT"
                     /locus_tag="ACHU0J_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1215916.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            22004..22693
                     /gene="mutH"
                     /locus_tag="ACHU0J_001152"
     CDS             22004..22693
                     /gene="mutH"
                     /locus_tag="ACHU0J_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1215917.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22762..23475
                     /locus_tag="ACHU0J_001153"
     CDS             22762..23475
                     /locus_tag="ACHU0J_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1215918.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23613..23831
                     /gene="ygdR"
                     /locus_tag="ACHU0J_001154"
     CDS             23613..23831
                     /gene="ygdR"
                     /locus_tag="ACHU0J_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1215919.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23939..24979
                     /locus_tag="ACHU0J_001155"
     CDS             23939..24979
                     /locus_tag="ACHU0J_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1215920.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="ACHU0J_001156"
     CDS             complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="ACHU0J_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1215921.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26195..28354)
                     /gene="aas"
                     /locus_tag="ACHU0J_001157"
     CDS             complement(26195..28354)
                     /gene="aas"
                     /locus_tag="ACHU0J_001157"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1215922.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28940..29971
                     /gene="galR"
                     /locus_tag="ACHU0J_001158"
     CDS             28940..29971
                     /gene="galR"
                     /locus_tag="ACHU0J_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1215923.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="ACHU0J_001159"
     CDS             complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="ACHU0J_001159"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1215924.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31362..32297
                     /gene="lysR"
                     /locus_tag="ACHU0J_001160"
     CDS             31362..32297
                     /gene="lysR"
                     /locus_tag="ACHU0J_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1215925.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="ACHU0J_001161"
     CDS             complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="ACHU0J_001161"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1215926.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33105..34523)
                     /gene="araE"
                     /locus_tag="ACHU0J_001162"
     CDS             complement(33105..34523)
                     /gene="araE"
                     /locus_tag="ACHU0J_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1215927.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8480 a         9510 c         8580 g         8105 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct cttcctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgctcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgt tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca aaaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcatgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcggttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaat cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcagata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta tggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gagttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacaattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agcttttggt
    14941 actggtttct gctggcgaga ctgttcattg atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcagcacca tcagcaccat cagcaccatc gcaaccatta
    15061 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    15121 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    15181 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    15241 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    15301 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    15361 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    15421 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    15481 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    15541 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    15601 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    15661 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    15721 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    15781 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    15841 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    15901 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    15961 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    16021 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    16081 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    16141 agcgaatatg cacggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca
    16201 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    16261 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    16321 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    16381 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    16441 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    16501 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    16561 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    16621 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    16681 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    16741 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    16801 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    16861 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    16921 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    16981 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    17041 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    17101 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    17161 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    17221 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    17281 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    17341 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    17401 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    17461 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    17521 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    17581 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    17641 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    17701 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    17761 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    17821 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    17881 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    17941 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    18001 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    18061 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    18121 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    18181 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    18241 gattcgcccg acgtgttgcc agccacattg caaaaatgaa ccccaccaga tacatcaggc
    18301 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    18361 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    18421 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    18481 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    18541 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    18601 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    18661 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    18721 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    18781 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    18841 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    18901 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gaccctcttt gccagatgcg
    18961 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    19021 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    19081 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    19141 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    19201 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    19261 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    19321 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    19381 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    19441 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    19501 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    19561 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    19621 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    19681 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa
    19741 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    19801 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    19861 gcaatcgctg acccagcgca cgtaaatcac cagcccgctc tttgagatag ttatcgctta
    19921 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    19981 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    20041 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    20101 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    20161 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    20221 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    20281 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    20341 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    20401 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    20461 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    20521 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    20581 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    20641 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    20701 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    20761 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    20821 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    20881 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    20941 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    21001 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    21061 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    21121 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    21181 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    21241 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    21301 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    21361 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    21421 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    21481 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    21541 cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca
    21601 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    21661 ttggcgaaag atcaaccaat gccgtgttta tccacagaat gtgccactaa gttaagcact
    21721 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    21781 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    21841 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    21901 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    21961 cggcacataa ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    22021 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    22081 ttatacattg ggagaactgg cggcacttga cgggctggtt acgccagaga atttaaaacg
    22141 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    22201 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    22261 tggtcgtccg ctggaaacaa cattcgtttg tgttgcccgg ttaacgggta atagcggggt
    22321 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    22381 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    22441 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    22501 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    22561 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    22621 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    22681 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    22741 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    22801 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    22861 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    22921 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    22981 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    23041 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    23101 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    23161 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    23221 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    23281 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    23341 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    23401 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    23461 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    23521 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    23581 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    23641 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    23701 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    23761 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    23821 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    23881 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    23941 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    24001 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    24061 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    24121 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    24181 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    24241 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    24301 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    24361 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    24421 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    24481 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    24541 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    24601 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    24661 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    24721 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    24781 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt
    24841 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    24901 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    24961 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    25021 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    25081 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    25141 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    25201 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    25261 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    25321 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    25381 cttcgctgcc gcacctgcgc caaccacgat accaatcgct accatcgcgt tgagataggt
    25441 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    25501 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    25561 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    25621 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    25681 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    25741 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    25801 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    25861 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    25921 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    25981 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    26041 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa
    26101 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    26161 cttcgaccac aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt
    26221 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    26281 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    26341 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    26401 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    26461 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    26521 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    26581 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    26641 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    26701 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    26761 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    26821 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    26881 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    26941 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    27001 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    27061 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    27121 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    27181 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    27241 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    27301 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    27361 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    27421 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    27481 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    27541 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    27601 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    27661 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    27721 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    27781 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    27841 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    27901 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    27961 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    28021 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    28081 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    28141 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    28201 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    28261 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    28321 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    28381 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    28441 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    28501 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    28561 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    28621 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    28681 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    28741 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    28801 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    28861 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    28921 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    28981 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    29041 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    29101 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    29161 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    29221 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    29281 tcgctgtgct gcgttggtcg tccatgccaa aatgatccca gatgccgatt tagcctcatt
    29341 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    29401 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    29461 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    29521 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    29581 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    29641 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    29701 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    29761 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    29821 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    29881 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    29941 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    30001 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    30061 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    30121 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    30181 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    30241 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    30301 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    30361 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    30421 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    30481 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    30541 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    30601 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    30661 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    30721 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    30781 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    30841 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    30901 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    30961 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    31021 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    31081 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    31141 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    31201 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    31261 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    31321 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    31381 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    31441 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    31501 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    31561 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    31621 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    31681 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    31741 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    31801 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    31861 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    31921 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    31981 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    32041 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    32101 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    32161 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    32221 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    32281 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    32341 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    32401 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    32461 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    32521 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    32581 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    32641 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    32701 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    32761 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    32821 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    32881 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    32941 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    33001 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    33061 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    33121 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    33181 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    33241 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    33301 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    33361 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    33421 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    33481 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    33541 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    33601 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    33661 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    33721 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    33781 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    33841 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    33901 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    33961 gctggtaaag caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta
    34021 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    34081 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    34141 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    34201 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    34261 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    34321 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    34381 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    34441 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    34501 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    34561 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    34621 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttatt
//
LOCUS       DAZPDE010000026        33123 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPDE010000026 DAZPDE010000000
VERSION     DAZPDE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.026"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(192..332)
                     /gene="yjjY"
                     /locus_tag="ACHU0J_001163"
     CDS             complement(192..332)
                     /gene="yjjY"
                     /locus_tag="ACHU0J_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1215928.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            428..1144
                     /gene="arcA"
                     /locus_tag="ACHU0J_001164"
     CDS             428..1144
                     /gene="arcA"
                     /locus_tag="ACHU0J_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1215929.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1203..2555)
                     /gene="creD"
                     /locus_tag="ACHU0J_001165"
     CDS             complement(1203..2555)
                     /gene="creD"
                     /locus_tag="ACHU0J_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1215930.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2613..4037)
                     /gene="creC"
                     /locus_tag="ACHU0J_001166"
     CDS             complement(2613..4037)
                     /gene="creC"
                     /locus_tag="ACHU0J_001166"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1215931.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4037..4726)
                     /gene="creB"
                     /locus_tag="ACHU0J_001167"
     CDS             complement(4037..4726)
                     /gene="creB"
                     /locus_tag="ACHU0J_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1215932.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4739..5212)
                     /gene="creA"
                     /locus_tag="ACHU0J_001168"
     CDS             complement(4739..5212)
                     /gene="creA"
                     /locus_tag="ACHU0J_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1215933.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5423..6292
                     /gene="robA"
                     /locus_tag="ACHU0J_001169"
     CDS             5423..6292
                     /gene="robA"
                     /locus_tag="ACHU0J_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1215934.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6289..6936)
                     /gene="gpmB"
                     /locus_tag="ACHU0J_001170"
     CDS             complement(6289..6936)
                     /gene="gpmB"
                     /locus_tag="ACHU0J_001170"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1215935.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6988..7508
                     /gene="yjjX"
                     /locus_tag="ACHU0J_001171"
                     /pseudo
     CDS             6988..7508
                     /gene="yjjX"
                     /locus_tag="ACHU0J_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(7593..7919)
                     /gene="trpR"
                     /locus_tag="ACHU0J_001172"
     CDS             complement(7593..7919)
                     /gene="trpR"
                     /locus_tag="ACHU0J_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1215936.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8009..9946)
                     /gene="sltY"
                     /locus_tag="ACHU0J_001173"
     CDS             complement(8009..9946)
                     /gene="sltY"
                     /locus_tag="ACHU0J_001173"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1215937.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10157..11824
                     /gene="ettA"
                     /locus_tag="ACHU0J_001174"
     CDS             10157..11824
                     /gene="ettA"
                     /locus_tag="ACHU0J_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1215938.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11931..12098)
                     /gene="nadS"
                     /locus_tag="ACHU0J_001175"
     CDS             complement(11931..12098)
                     /gene="nadS"
                     /locus_tag="ACHU0J_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR1215939.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12132..13364)
                     /gene="nadR"
                     /locus_tag="ACHU0J_001176"
     CDS             complement(12132..13364)
                     /gene="nadR"
                     /locus_tag="ACHU0J_001176"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1215940.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13385..14767)
                     /gene="radA"
                     /locus_tag="ACHU0J_001177"
     CDS             complement(13385..14767)
                     /gene="radA"
                     /locus_tag="ACHU0J_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1215941.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14816..15784)
                     /gene="serB"
                     /locus_tag="ACHU0J_001178"
     CDS             complement(14816..15784)
                     /gene="serB"
                     /locus_tag="ACHU0J_001178"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1215942.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15890..16534
                     /locus_tag="ACHU0J_001179"
     CDS             15890..16534
                     /locus_tag="ACHU0J_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1215943.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16562..17577
                     /gene="lplA"
                     /locus_tag="ACHU0J_001180"
                     /pseudo
     CDS             16562..17577
                     /gene="lplA"
                     /locus_tag="ACHU0J_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            17609..17872
                     /locus_tag="ACHU0J_001181"
     CDS             17609..17872
                     /locus_tag="ACHU0J_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1215944.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(18033..18752)
                     /gene="deoD"
                     /locus_tag="ACHU0J_001182"
     CDS             complement(18033..18752)
                     /gene="deoD"
                     /locus_tag="ACHU0J_001182"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1215945.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18809..20032)
                     /gene="deoB"
                     /locus_tag="ACHU0J_001183"
     CDS             complement(18809..20032)
                     /gene="deoB"
                     /locus_tag="ACHU0J_001183"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1215946.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20084..21406)
                     /gene="deoA"
                     /locus_tag="ACHU0J_001184"
     CDS             complement(20084..21406)
                     /gene="deoA"
                     /locus_tag="ACHU0J_001184"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1215947.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(21533..22312)
                     /gene="deoC"
                     /locus_tag="ACHU0J_001185"
     CDS             complement(21533..22312)
                     /gene="deoC"
                     /locus_tag="ACHU0J_001185"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1215948.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22570..24111
                     /locus_tag="ACHU0J_001186"
     CDS             22570..24111
                     /locus_tag="ACHU0J_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1215949.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            24083..24946
                     /locus_tag="ACHU0J_001187"
     CDS             24083..24946
                     /locus_tag="ACHU0J_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1215950.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25160..25942)
                     /locus_tag="ACHU0J_001188"
     CDS             complement(25160..25942)
                     /locus_tag="ACHU0J_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1215951.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(25939..27012)
                     /locus_tag="ACHU0J_001189"
     CDS             complement(25939..27012)
                     /locus_tag="ACHU0J_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1215952.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27134..27295)
                     /locus_tag="ACHU0J_001190"
     CDS             complement(27134..27295)
                     /locus_tag="ACHU0J_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1215953.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27412..28017)
                     /gene="osmY"
                     /locus_tag="ACHU0J_001191"
     CDS             complement(27412..28017)
                     /gene="osmY"
                     /locus_tag="ACHU0J_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1215954.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28410..29999)
                     /gene="prfC"
                     /locus_tag="ACHU0J_001192"
     CDS             complement(28410..29999)
                     /gene="prfC"
                     /locus_tag="ACHU0J_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1215955.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30090..30767)
                     /gene="yjjG"
                     /locus_tag="ACHU0J_001193"
     CDS             complement(30090..30767)
                     /gene="yjjG"
                     /locus_tag="ACHU0J_001193"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1215956.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30782..31228)
                     /gene="rimI"
                     /locus_tag="ACHU0J_001194"
     CDS             complement(30782..31228)
                     /gene="rimI"
                     /locus_tag="ACHU0J_001194"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1215957.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31197..31610)
                     /gene="holD"
                     /locus_tag="ACHU0J_001195"
     CDS             complement(31197..31610)
                     /gene="holD"
                     /locus_tag="ACHU0J_001195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1215958.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31713..32744
                     /gene="rsmC"
                     /locus_tag="ACHU0J_001196"
     CDS             31713..32744
                     /gene="rsmC"
                     /locus_tag="ACHU0J_001196"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1215959.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33021..33107
                     /locus_tag="ACHU0J_001197"
     tRNA            33021..33107
                     /locus_tag="ACHU0J_001197"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33055..33057,aa:Leu,seq:cag)
BASE COUNT         7813 a         9108 c         8450 g         7752 t
ORIGIN      
        1 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
       61 ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
      121 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
      181 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
      241 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
      301 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
      361 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
      421 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
      481 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
      541 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
      601 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
      661 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
      721 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
      781 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
      841 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
      901 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
      961 aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccgcacga
     1021 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
     1081 gccggaaatc atcgccacca ttcacggtga aggttatcgc ttctgcggtg atctggaaga
     1141 ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
     1201 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
     1261 ggcagtgaaa acgcatacca gtctatatta cgagtcacaa acatcatgcc ggccagcgcc
     1321 accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag cagtccccac
     1381 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
     1441 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
     1501 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
     1561 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
     1621 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
     1681 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc
     1741 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
     1801 tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt tggctggtag aaaatcacct
     1861 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
     1921 accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg caggttctgc
     1981 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
     2041 gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac cacaccaata
     2101 ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat atttggcgcg
     2161 ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
     2221 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
     2281 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
     2341 tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac gagtttttgc
     2401 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
     2461 acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacaag
     2521 gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc aatggcagca
     2581 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgtagc
     2641 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
     2701 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
     2761 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
     2821 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatgcaaccg
     2881 ctctcgggag taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
     2941 gccggttcag cagcaacatt aacctcggtg ggcataacat gcaaagtgat gtttttttct
     3001 gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac atcaacagca
     3061 gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagc
     3121 gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
     3181 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
     3241 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
     3301 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
     3361 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
     3421 atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccaacaaaat ggcgctggcc
     3481 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
     3541 acgctcaaaa cgccaataag ccgcgagccg cccataatcg gcgcggcaac atacatcacc
     3601 gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg accacgcaac
     3661 gttagccaga cgtcattcca gcgcgaataa tcctgtccaa cggctttatt tgccgaatcg
     3721 aacaatactt tgccatgcgc atcggtcata tagacatgat attcattgcg cactttgtta
     3781 atgccaccaa tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
     3841 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc aagtaacgtt
     3901 gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
     3961 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
     4021 cgcatgccga tacgcattac aagcccctca ggctatatcc catgccgcga tgagtattaa
     4081 tcggtgaaag atcggggttg atggcgcgca gcttggcacg cagcgtttta atgtggatat
     4141 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
     4201 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
     4261 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
     4321 gaccaatacg gatgacggga gacggcgtcg agaacttctt cacccgacgc agtaaggtgc
     4381 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
     4441 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
     4501 acgccggata gagcgccagt aactggcggc acaattcaaa gccactgata tcaggcaaac
     4561 caacgtcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaaac
     4621 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
     4681 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
     4741 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
     4801 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
     4861 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
     4921 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatggccg
     4981 catcggaggt atcttctgcc aggcccaatc cccctttaat accaccggtt ttcgcccggc
     5041 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
     5101 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
     5161 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
     5221 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
     5281 caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
     5341 actggatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
     5401 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
     5461 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
     5521 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
     5581 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
     5641 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
     5701 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
     5761 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
     5821 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
     5881 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
     5941 cattccgccg gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
     6001 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
     6061 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
     6121 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaatct
     6181 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
     6241 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
     6301 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
     6361 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
     6421 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
     6481 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
     6541 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
     6601 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
     6661 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
     6721 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
     6781 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
     6841 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
     6901 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
     6961 acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac caccaatccc
     7021 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
     7081 attgcatccg ttgccgtcga gagcggtgta ccggaacaac cctttggcag tgaggaaacg
     7141 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
     7201 tgggtggcga ttgaagctgg cattgatggc gacagcactt tcagttgggt ggtgattgaa
     7261 aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc cgttacctgc ggtgattctg
     7321 gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc gcgttatacc ggtattgatg
     7381 agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca
     7441 gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc gtgtatcagg
     7501 cattgtagga cggataaggc gttcacgccg catccgacat tcggtgcacg atgcctgatg
     7561 cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa cacctcttcc
     7621 agccactgac gcagctccac gggcgcggct ttcaggctgt tagatccacg cgtaatcgtc
     7681 gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc acgcaacagc
     7741 tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt cagcatcagg
     7801 tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc gacaaaacgt
     7861 aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg ttgggccata
     7921 atatgtcgcc attgttagcg ggggaagcaa aatgactccc ccggttgtac tcgctaaaga
     7981 gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc gtggcgctca
     8041 tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc atcataagcc agcacgttct
     8101 tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca gcgtcgatac
     8161 gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta taagctgctg
     8221 aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa
     8281 tgttgatgtt cgtttccgga tccagcaatt gcccagggct gctataacca ggaatagaga
     8341 acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg ctggccccta
     8401 ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc atcgcatagc
     8461 tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga
     8521 atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg ctaagatccc
     8581 accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt gacttgctct
     8641 tcaccagatt ggcccactcg ctacgcgcgg tattatcgag attccagtac atcaactcgc
     8701 gcacgcgcgc catctccggc ccctgagtca gggtgctgtc aacattctgc ggcgctttat
     8761 caatcttcag ctcatactct tcaccgatgc gttgtgctgc aaccatcggg tagaaaccac
     8821 gctgttgtat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg cgttccagca
     8881 ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc ggcagacgcg
     8941 ccagccaggt attcaggccg cgacgatcgc cggtgccaag cgccattcgc acacggcgtt
     9001 caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc gcctgctcat
     9061 cggtgacatc attgcccatc aaacgccagg cgacgatatc gcgcagctcc tgaatctgat
     9121 cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca ttttcggcat
     9181 cctgccgcgc cacactggca aacgccaccg ccgccatttg acgggtaaaa tcggtcgcgc
     9241 cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg
     9301 aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc aggcctgtgt
     9361 tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct tgtttacctg
     9421 acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc
     9481 acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt
     9541 agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct aacaagccac
     9601 gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag
     9661 gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcaggt
     9721 catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc agtccaggca
     9781 tcatttgttc gaccacatcc atctgtcgat tatcccaggc ctgcttgatt tgcgcgtaac
     9841 ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga
     9901 caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc gcatcctcta
     9961 agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg
    10021 ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat cgaccaactg
    10081 ggattaggcg gggaaacagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat
    10141 agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt
    10201 tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat
    10261 tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg ctgcgcatta tggcgggcat
    10321 tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttatctgcc
    10381 gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc
    10441 agaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg ccgatccgga
    10501 tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca
    10561 cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctgc gtctgccaga
    10621 ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtgg cgttgtgccg
    10681 cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc
    10741 cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc gaaggcaccg tggtggcgat
    10801 tactcacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac tggaccgcgg
    10861 tgaaggaatt ccgtgggaag gtaactactc ctcctggctg gagcagaaag atcagcgtct
    10921 ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag agctggagtg
    10981 ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg cacgctttga
    11041 agagctgaac agcaccgaat accagaaacg taacgaaacc aacgaactgt ttattccacc
    11101 tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat cctacggcga
    11161 tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcgatcg tcgggatcat
    11221 cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctccggtc aggaacagcc
    11281 ggacagcggc accatcactt tgggtgaaac ggtgaaactg gcatcggttg atcagttccg
    11341 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat
    11401 gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
    11461 ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt gagcgcggtc gtctgcatct
    11521 ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
    11581 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat
    11641 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca
    11701 ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaaaaa
    11761 acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc gtattgcgaa
    11821 gtaatgcgta aaatgccgga tgcggcgcga acgccttatc cggcctacaa aaccagcaat
    11881 ttcaatacgt tgtaaaactg taggcctgat aagacgcggt aagcgtcgca tcaggcattg
    11941 agcaccgaat gccggatgcg gcgtgaacac cttttccggt ctacaacagc cttagcatcc
    12001 tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac cttgtttaat ctcgacagcc
    12061 tcttccagag aggttttcaa ctcatcaaaa aaactcattt tatacctcgc ctttgagcag
    12121 tttcgtcacg gttatccttg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    12181 aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    12241 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    12301 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    12361 tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    12421 gtagtgacaa aatcggtgtc aataaacgcc actttattgg cgtatttcac tgcaaaatca
    12481 atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg aatactgcaa tgcgatttca
    12541 tcaccgccga ggtgtgaaaa gacataatcg cggccatatt cccacgcact ggtggtgttg
    12601 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc gccaaggatc
    12661 gccacggtac gcacaaaaaa cggcttcact tcggtaggaa tatattccca gtagcggaac
    12721 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    12781 accgtctcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    12841 atcaggtccg gctggatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    12901 tcccagccat gcggatacgg ctccatgccc tcttcgttga aagcatgaat acggatattt
    12961 ttctgatatt taaaggtttg caatagccag cgcagacgat ccggcaccgt gggctgctgc
    13021 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    13081 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    13141 gggtagaact taccgaatac gacaccaatg gttttcttct gccggggaaa ttcaagcccc
    13201 aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    13261 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    13321 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    13381 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    13441 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    13501 cgtgtttcgc cgcttcagaa atccgctcct ggccactggg caccgggcgg atctcccctg
    13561 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    13621 aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc ggttaccttc acgccgccga
    13681 ccacgttcac aaacacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    13741 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    13801 ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    13861 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    13921 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    13981 cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg ggagtcggca tcgccatcca
    14041 gaagcaccga acagtcgata cagtgttcca gcactttcgg gccagccagc gaaccatctt
    14101 tggttacgtg tccaaccatg acaatcgcca caccgcgcgt tttggcgaaa cgcgtcagat
    14161 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    14221 gcatcacctg gatcgagtca attaccatca gcttcggttg ctcttcttcg gcaatcaggc
    14281 agatctgctc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    14341 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc ggtgacatac agcgttttca
    14401 tctgctgggc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc gcaccagggt
    14461 taccgccaat cagaatggca cttcctggca ccacgccgcc tcctagtacg cggtcgaact
    14521 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    14581 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    14641 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    14701 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    14761 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca ggcaattact
    14821 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    14881 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    14941 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    15001 tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt gtactgcgcg tctacgatgt
    15061 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    15121 ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg ccggaggcaa
    15181 tcgccacttt ccagcccagc gtttccagct tgagcaccag ctgcgttaag cctggcatca
    15241 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    15301 cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttacct
    15361 ccgccaccat ctcgcccgtt ccggccagct tggcaatttc atcaatacat tcaatctgga
    15421 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    15481 ccagcggggc gacatccagc tgcgcttcgt gggccaggcg tgtagcccgt gcggtgagtg
    15541 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    15601 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    15661 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    15721 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    15781 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    15841 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    15901 aaaactgaaa ttccggctac atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    15961 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    16021 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    16081 gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt taacagatga
    16141 aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cacgttctgg
    16201 cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt aaaaaagcag gcggctattt
    16261 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    16321 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    16381 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    16441 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    16501 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    16561 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    16621 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    16681 tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    16741 gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc ggtggcggcg cggtgttcca
    16801 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaactat
    16861 ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    16921 acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc aaagtctcag gctcggccta
    16981 tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg ctactcaatg ccgacctcag
    17041 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacttc
    17101 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggctcacca tgagcaggtt
    17161 tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcacgtgga agcggaaatc
    17221 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgtcagagt
    17281 agctgagagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
    17341 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
    17401 gtatttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc
    17461 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
    17521 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
    17581 gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg aacgggagat
    17641 tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
    17701 attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaacagat
    17761 cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa tttatggttg
    17821 tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagattc
    17881 gtgaatagat acttgaatgt tttttatttt ctatccatat gaaaaataat agtaaatcta
    17941 taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc cactccggct
    18001 taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag cagaacggat
    18061 tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtctgc
    18121 tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc gccaaactct
    18181 gcagccacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc gtatttttcc
    18241 atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttaccc
    18301 acgcgagcat caacgcccag tgctttagct gcatctactg cgttacgcac catgtcaaag
    18361 tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt ggaatcggtg
    18421 caggccccca taccgataac gacgtcgcgc agttttacgt gcggcagaac tgcgccacag
    18481 gaaccaacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt ggtgtagatg
    18541 gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc tttgtaagta
    18601 ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt
    18661 tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcgaaatcg
    18721 cccatttctg cattaatgtg tggggtagcc attgttttat cctttttgtg acataacaaa
    18781 ggcggagtca acgctccgcc caaatccatc agaacatggc tttgccatat tccatatcag
    18841 aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca cggtgaccca
    18901 gtgagcccgg ttttactttc gggccatata ccagtaccgg aatgtgttca cgcgtgtggt
    18961 cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt gaggatcagg atgtcgtcat
    19021 cgcgcagcag agacatcagc tccggcagac ggcggtcgaa cagttccaga cccgcagcat
    19081 aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga
    19141 cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca
    19201 agccagtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct gcaattttac
    19261 cgacagaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg gtcggtgctg
    19321 gcggctcaac agccaagtcg tgacggttac cggtacgctg gaagttacca gctttgtcac
    19381 cgataaacgg acgagcgata acacgaccga tattgtagcc gccgttggtc agctcttcac
    19441 gggcgatttc gcacagttcg tagagtttat ccagaccgaa agtttcttca tggcaggcaa
    19501 tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc atgtgctctt
    19561 cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg taacccggca
    19621 gattagcgcg ttcgaccagt ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg
    19681 agaaatatcc ccactcaaac agaaccggga caccggcgat ttcccagtga ccagacgggg
    19741 tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc
    19801 cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc agccccagac
    19861 gggtcagatt tggcagattg agcggccctt tacgaccatt atcagcttcg cctttggcac
    19921 aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt tctgcatctt
    19981 ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc atatgttctc
    20041 cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg ctgatacggc
    20101 gatagacagt tggtgtgctt tccggtgcgt tatcggcaag tttaattgcc gctttcaccg
    20161 ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag gataaccgcc agcggacgct
    20221 gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa gccgacgctg taatcgatgg
    20281 tgtcagatgc ctgacgacga ccgccgccca ttgcaactac tgccatcccc agcgcgcggg
    20341 tatccatttc actgacaaaa ccttcggtat cagcatagac tgctttcgtc agcatcgctg
    20401 tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt gccgctacca
    20461 tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg
    20521 cttcggcgtc atctttcgcc agtttgccgg agatcaacat ctccacgcac agcgccatcg
    20581 tgacatcaaa cagacgcggg ttacgatact cacccgtcag gaactgcacc gcttcacgaa
    20641 cttcaaccgc gttacctgca ctggaggcca gtacctgatt catgtcggtg agcagcgcgg
    20701 tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg gcttcagaga
    20761 gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc agcgcgtcca
    20821 gaccttccgc cagtttcttc gccaggatcg aggcagtgat cagcgggatg gagtccacgg
    20881 ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc cggagccagt gaactggtct
    20941 gaccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg tcatccggga
    21001 aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta tgaccgaggc
    21061 cgcgaccgga gatcatcgga atatagccgc cgcaggctgc gaccatcggc cccaacatca
    21121 gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc aacaatcggg ccattcagat
    21181 gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc agcgagacac
    21241 gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca atctgccctt
    21301 cggaaatagt gttgtcgcga ataccgttga taaagaaacg aatttcttca tcgctcagcg
    21361 catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta acctcccgag
    21421 gaaaagagta tgaggcggct tttgccggat gcgtctacgc cttatccggc ctacgggtag
    21481 atacgtctcg taggcctgat aagcgcagcg catcaggcgt aaagcatctt acttagtagc
    21541 tgctggcgct cttaccgtcg ccgtggccca gcgctttcag caggcttgcc agcaggctgg
    21601 aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc accgaacagt tcatctgcga
    21661 tggcgagata tttctgcgca tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa
    21721 cggttttttc tacgcccata tcacggatca cttccatcat gatgcgagcg ctttccggcg
    21781 tcgcgttcac agccacttta ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg
    21841 agatttcaga cgctttacgg atcagcgctt cgtctttcag ttcgccggtt tcgatgatca
    21901 ctttcagcag tacattcgct gccgcgcaag cctctttaca ggctttcacc aggtcaaaac
    21961 caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa caccacgtca acttcatcag
    22021 caccgtaggc gattgccgca cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt
    22081 gtgggaagtt ggttaccgta gcgatacgga tttccggggt gccctgctct ttcagagttt
    22141 tgcgagcaat cgggataaaa cgaggataga tacagatagc ggcggtattg ccgaccggag
    22201 ttttggcctg atgacacaga gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg
    22261 tcaggtccat caatttcagt gcacgcaggc tgcttgcttt cagatcagtc atttcattct
    22321 cctggcttgt cgccaataaa attcaccttg cgagtttgtt agtattctaa catctactcc
    22381 gcaacacact tcgatacaca tcacaattaa ggaaatctac ttacaagttt gcatcactgt
    22441 aatgcgatct ggttcaaata attcactttc aaatgaatgc gtcagtggtg gcaaacgcat
    22501 caggatcaaa gtgaacatca cgaaacttct tacaatggcg catcaccttc ggcataaaag
    22561 gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc
    22621 accagcccag tgcttagccc ggagcagaag cgccattttc tggcactgga agcagaaaac
    22681 aatctgcctt acccacagct tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc
    22741 tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct atgtcttacc cgattacgct
    22801 cgttttctgg cgaacggttc cgaatggctg gagctggaag gcgcgaaaga tcttgatgac
    22861 gcactctctc tgctgaccat tctttaccac cacgtaccgt cggtcacatc gatgccggtc
    22921 tacctggggc aactggatgc gttgttgcaa ccgtatgtta gaattctaac acaaaacgag
    22981 atcgatgttc gaataaaacg tttctggcgt tacctcgaca gaaccctgcc agatgccttt
    23041 atgcacgcca atatcggccc gtctgattcg cccattaccc gcgcgatctt acgtgcagac
    23101 gcgcaggttt caccgaacct gacctttatc tacgatcctg aaatcacccc tgatgacctg
    23161 ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc caacggtccg
    23221 gtgcatgata aaattttcac aaaagggggc tacgggattg tgagctgtta caactcactg
    23281 ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat tgccgagcgc
    23341 agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca gcagcagatc
    23401 gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt tgagaatagc
    23461 ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt tggcatgtat
    23521 gggctggcgg aagcggttaa cttgctgtgt gagaaagaag ggattgccgc gcgctacggt
    23581 aaagaagccg ccgcaaatga agtgggttat cgcatcagcg cgcaactggc ggagtttgtc
    23641 gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc acagtcgggg
    23701 atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggcga tgagccagat
    23761 ccgatcaccc atctgcaaac tgtcgccccg catcatgctt attattattc cggcatcagc
    23821 gacattctga cgctcgacga aaccatcaaa cgtaacccgc aggcactggt acagctttgc
    23881 ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg
    23941 gttcgcgtta ccggttatat ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt
    24001 tcacgcacca acaccacctg gctgggcgaa gaagccgcac gcaacactcg tattctggaa
    24061 cgccagccgc gcgtgataag ccatgaacag cagatgcgct ttagtcagta agattatccc
    24121 cttctcctgc gttgacgggc caggcagtcg tctggctctg tttttgcagg gctgcaatct
    24181 gcgctgcaaa aactgtcaca atccgtggac gatgggacgt tgcaatgact gtggggagtg
    24241 cgtgccacag tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc
    24301 tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt ccgcaacatg ccacaccaat
    24361 ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg cgcaaagcgg tgctgtttat
    24421 tgaagggata acggtaagcg gcggcgaagc cacgacccaa ctgccgtttg tagtggcgct
    24481 gtttactgct atcaaaaacg atccgcaact gcgccatctc acctgtctgg tggacagtaa
    24541 cggtatgttg agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct
    24601 cgatctcaaa gcatggggta gcgaatgtca tcaacaatta accggacgcg ataatcaaca
    24661 gattaagcgc agcatctatt tgctggcaga gcgcggcaag ctggcggaac tgcgtttgct
    24721 gatgatccca ggccaggtgg attatttgca acacatcgaa gaactggcgg cgtttatcaa
    24781 gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat gcccacggcg tgtatggcga
    24841 ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag ccgttggctg atgcattaaa
    24901 ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat ttgtgacatg ccggatacgg
    24961 cctacggttc ggcacagact tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat
    25021 tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt cggcacaaac
    25081 ctgtaggcat gataagacgc gttaagcgtc gcatcaggca ttatgcgccg actgccggat
    25141 gctgcctacg aagggcaaac tacccacgaa cgttaaacac cgcatacgtg ttattaagca
    25201 acacttcggc aatctcatcc gcgggttccg ggcgtaactc gcaaagcacg gcaaacacac
    25261 gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc gttgagcggc atatccggcg
    25321 catcggtttc cagcaacaac gatgccagcg gtaattttgc gatgacatcg cgggttttac
    25381 tggcgcgtgg ataggtgata gtaccgccta cgccgatttt gtagcccagc tgtacaaacc
    25441 gttcagcctg ttgctggctg ccagaaaatc cgtggacaac accagcacgc ggcaaatcgt
    25501 ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg tgaatgcagg atcaccggca
    25561 gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa ccactgctgc ctctcaaatt
    25621 gcggatcgtc gccgaagaga tccagaccga tctcccccac cgccaccacc ttcgccggac
    25681 gcctttccag cgcctgctgt agctgctcaa gagacacatc gctatgtttt tccaacatac
    25741 caggatgcaa gcccaatacg gcatacagcg gttgataatt ttccgctaac gcctgcacac
    25801 gggcaaaatt ttccgcctct gtcgccggaa caatgatctt gcctacgccc gcttgtgccg
    25861 cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtgag
    25921 tgtcgataaa acggcaaatc aagccagatc ctcattgtta aatgtcgggt cgttcgcctg
    25981 cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg actggcgcag gcggaatgac
    26041 aatcgattcc ggcgtcacca ctggcaccag atggcgggta agcggcgctt tttcagttaa
    26101 tagcttgcca accgtggcga ggaaataacg accgcataaa cgcccaagtt tatagtcttc
    26161 acgcagcgcc ggaatccgac tgccaagcgc gatactatgt aatggcttcg gcggataaat
    26221 ttcggatatc cgcagcttgc ccggtggttt ctcaataaat tgctgaatgt cacgatagct
    26281 ggtttcatga tgctgcacca gattgaccag cggctgcagg ctactgtcac ccagccagcg
    26341 ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt gacggcacag tgcggatgac
    26401 gaccaacgtt ttagcgccct gccttgccgc ctctttaacc ggaatcgcat cactgatccc
    26461 gccatccagg tagttaatgc cttccagcga cactccgcta cgataaaagc caggtatcgc
    26521 actggaggcg cgaatcacat ccagccagtt ttgtttggtt ggtaaaaagt aattcggcgc
    26581 gtagtcatcc tgacgacagg cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc
    26641 ggcggtatcc atttgcaacg gcatctggct tgcggtggcc tccaccagcc agtcgagatc
    26701 gataagattt cctccacgga caaagcgcaa tggatcgaaa aattcgcgtt ttgtggtata
    26761 gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag ataaacgccg agaggttctg
    26821 cgccccggca gatgtgccga gataaagatc gaaaggatta aactgcgcgc gcataaactc
    26881 atccagcacg ccagccgtga aaattccacg ctgtccgccg ccttcgcaca ccagagctat
    26941 tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat
    27001 tcgctgcccc acttctgcct tcctgttttt gttgtgaaag aaagacaaag taacgcaatt
    27061 tattattcgc taaaaccgca aaagccagtc cgctggactg gctttggata aaattttctt
    27121 aatggtctga aatctagggt cgttttcggc ccatgaacaa actcactagg aacagaataa
    27181 tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt accggccaga ccaccaaacc
    27241 caagtgcggc ggcgattaac gcgataacca gaaatatgat gccccaacga aacataagcg
    27301 tctcctttac catagttaat gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat
    27361 atttttagtg tggtgcacat cgcgcctccc gacaaagttc gggaggacga attacttagt
    27421 tttcagatca tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg
    27481 gtcactttgt gcctgagaat ctacggtacc ggagagctga accacgccat cggtggtttc
    27541 aactttcact ttacgtgaag ggacgatatc gtccgccagc agtttggctt tgatttcact
    27601 ggtggtggcg gtgtcacccg cgtagccctt caccgagcct tctttagcgt cgcgaacgtg
    27661 cagtttgtcg ctgacagagg tcacgccttc aacgcctttc gccactttca ctgcctcttc
    27721 ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac
    27781 agagatatcg gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt
    27841 gatggcgctg tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc
    27901 tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc
    27961 agaggtcaac attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga
    28021 tttattcctg tatgtttgct cgtaatttga gcctagcaac acgaggttgc attgctgaat
    28081 agggagagac ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgggtgat
    28141 gaataacgac cattacagcc tctgaatcag ttattaatat cggtagaatg acaatcgacg
    28201 gctttagata ctaatatcta cgcattgaac ggtatttaac gccatcagaa atgtcatcac
    28261 tttgttaaat atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa
    28321 aagtgaatgt tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacagggcg
    28381 cagcggctac cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg
    28441 tccggataac gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg
    28501 ttatcgccgc catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc
    28561 ttcgcgtctg cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct
    28621 tcaacgttgt attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc
    28681 gcaccaacga tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg
    28741 gaaagctgta ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata
    28801 cgacggaaca gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg
    28861 gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga
    28921 tacgcttctt caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc
    28981 acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt accggacacc
    29041 acacgcataa acgccacgcg gtcgcggtgt ttcggatcca tgttggcctg aattttaaat
    29101 acgaagccgg taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc
    29161 atcggcgcag gcgcccactc caccaggcca ttcagcatat gatcaacgcc gaagttaccc
    29221 agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac
    29281 tcgttagacg cgcctttaac cagttccagt tcgtcacgca gctgctgtgc cagatcttca
    29341 ccaaccgcag catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg
    29401 gtgtgccctt taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct
    29461 ttaaacagct tgccgcagcc aattggccaa gtgatcggtg cgcagccaat tttcagctcg
    29521 ttctcaactt catcgagcag ttccatcggg tcgcggatat cacggtcaag tttgttcata
    29581 aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct
    29641 tcaacacctt ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga
    29701 taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg
    29761 tgatacggaa actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc
    29821 atccagtccg acttagcgtg ctggttggaa ccacggcctt ttactgtacc ggcggtctga
    29881 atggcctgtc cgaacagcag caccttctcg gtgatggtag ttttaccggc gtccgggtga
    29941 gaaataatgg caaaagtgcg gcgcttcgcc acctctttca aataaggaga caacgtcata
    30001 attctttctt cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc
    30061 tattttaccc atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg
    30121 tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca
    30181 ttcagccagc aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg
    30241 tcgccaacca tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg
    30301 aaaattttct tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag
    30361 taatcacgca ggcccgtccg ttccagacgc acttgctgca aggcgctaaa accgttggtg
    30421 atgatgccga ttttagcgtt gctacgaatg gcgttaagca gagaaaccgc gcccggcaac
    30481 ggcgtacaga tttccgccat cgcattaata aaggcatcgt taagcaagcc tgcttcaact
    30541 ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg
    30601 ccgttttgat aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca
    30661 gcggtaaagg tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag
    30721 gtaaacagcg tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt
    30781 cttacatact gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt
    30841 agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg
    30901 cagcatttga ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt
    30961 cgatcagatg ttccagcagc gccctaccca gcccctgacg ctgatagtcg ggatcgaccg
    31021 caatattgaa caatgtagct tcatccagca ccacttgcgt aatcgcaaac gccgccattt
    31081 tgccgttttg cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt
    31141 tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag
    31201 tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt
    31261 gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccaat
    31321 gatagcggtt cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc
    31381 gcgatttttt ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga
    31441 acatcgctca ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg
    31501 acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac
    31561 cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc
    31621 tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat
    31681 aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag
    31741 tcttgctgcg tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac
    31801 aggatgacct gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc
    31861 accattggca ggtgttaagc tgccagatgg gggataacgc ccgttttagt ctggtcgcca
    31921 cggcgaatga tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg
    31981 aagcccagtt ccagttgatg aatttacttt ctctgctgcc agtggggaca gatatttttg
    32041 tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca gattatgcgc
    32101 cgttgaataa agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac
    32161 agccagtatt tgatgcggat aaattctggg gcgaatacag cgtcgatggc ctgacggtca
    32221 aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct
    32281 cgacgttaac tccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac
    32341 tttcagttgc ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg
    32401 ccccggcggt agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct
    32461 ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga tatgatcatc tccaacccgc
    32521 cgttccacga tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgtggcgcgg
    32581 tgcgtcatct taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttacc
    32641 cggacgtgct ggatgagaca tttggcttcc atgaagttat cgcgcaaacc gggcgcttca
    32701 aggtatatcg cgccattatg acccgccagg cgaagaaagg ttgagtatct gatatctgtc
    32761 gccgcatacc gtgtcggatg cggcgtgaac gccttatccg acctacaaaa cctgcacgtt
    32821 agcccttcgt aggccagata agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc
    32881 ctaccaaacc tgcacgctag tttcctgatg gacatttttc cagcaattac acatcaggcg
    32941 ataattaact attgacgaaa agctgaaaac cactagaatg cgcctccgtg gtagcaattc
    33001 tttttaagaa ttgatggtat gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg
    33061 ttagtgtcct tacggacgtg ggggttcaag tcccccccct cgcaccaaaa atcacgttga
    33121 tat
//
LOCUS       DAZPDE010000027        32325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPDE010000027 DAZPDE010000000
VERSION     DAZPDE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.027"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            223..1596
                     /gene="aroP"
                     /locus_tag="ACHU0J_001198"
     CDS             223..1596
                     /gene="aroP"
                     /locus_tag="ACHU0J_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1215960.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1639..2493)
                     /gene="ampE"
                     /locus_tag="ACHU0J_001199"
     CDS             complement(1639..2493)
                     /gene="ampE"
                     /locus_tag="ACHU0J_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1215961.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2490..3041)
                     /gene="ampD"
                     /locus_tag="ACHU0J_001200"
     CDS             complement(2490..3041)
                     /gene="ampD"
                     /locus_tag="ACHU0J_001200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1215962.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3129..4022
                     /gene="nadC"
                     /locus_tag="ACHU0J_001201"
     CDS             3129..4022
                     /gene="nadC"
                     /locus_tag="ACHU0J_001201"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1215963.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4225..4665
                     /gene="ppdD"
                     /locus_tag="ACHU0J_001202"
     CDS             4225..4665
                     /gene="ppdD"
                     /locus_tag="ACHU0J_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1215964.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4675..5822
                     /locus_tag="ACHU0J_001203"
                     /pseudo
     CDS             4675..5822
                     /locus_tag="ACHU0J_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            5812..7014
                     /gene="hofC"
                     /locus_tag="ACHU0J_001204"
     CDS             5812..7014
                     /gene="hofC"
                     /locus_tag="ACHU0J_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1215965.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7049..8092)
                     /gene="guaC"
                     /locus_tag="ACHU0J_001205"
     CDS             complement(7049..8092)
                     /gene="guaC"
                     /locus_tag="ACHU0J_001205"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1215966.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8254..8292)
                     /locus_tag="ACHU0J_001206"
                     /pseudo
     CDS             complement(8254..8292)
                     /locus_tag="ACHU0J_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8317..8937
                     /gene="coaE"
                     /locus_tag="ACHU0J_001207"
     CDS             8317..8937
                     /gene="coaE"
                     /locus_tag="ACHU0J_001207"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1215967.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            8937..9680
                     /gene="zapD"
                     /locus_tag="ACHU0J_001208"
     CDS             8937..9680
                     /gene="zapD"
                     /locus_tag="ACHU0J_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1215968.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9690..9887
                     /gene="yacG"
                     /locus_tag="ACHU0J_001209"
     CDS             9690..9887
                     /gene="yacG"
                     /locus_tag="ACHU0J_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1215969.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(9987..10376)
                     /gene="mutT"
                     /locus_tag="ACHU0J_001210"
     CDS             complement(9987..10376)
                     /gene="mutT"
                     /locus_tag="ACHU0J_001210"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1215970.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10436..13141)
                     /gene="secA"
                     /locus_tag="ACHU0J_001211"
     CDS             complement(10436..13141)
                     /gene="secA"
                     /locus_tag="ACHU0J_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1215971.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13203..13715)
                     /gene="secM"
                     /locus_tag="ACHU0J_001212"
     CDS             complement(13203..13715)
                     /gene="secM"
                     /locus_tag="ACHU0J_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1215972.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(13946..14863)
                     /gene="lpxC"
                     /locus_tag="ACHU0J_001213"
     CDS             complement(13946..14863)
                     /gene="lpxC"
                     /locus_tag="ACHU0J_001213"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1215973.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(14964..16115)
                     /gene="ftsZ"
                     /locus_tag="ACHU0J_001214"
     CDS             complement(14964..16115)
                     /gene="ftsZ"
                     /locus_tag="ACHU0J_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1215974.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16176..17438)
                     /gene="ftsA"
                     /locus_tag="ACHU0J_001215"
     CDS             complement(16176..17438)
                     /gene="ftsA"
                     /locus_tag="ACHU0J_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1215975.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17435..18265)
                     /gene="ftsQ"
                     /locus_tag="ACHU0J_001216"
     CDS             complement(17435..18265)
                     /gene="ftsQ"
                     /locus_tag="ACHU0J_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1215976.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18267..19187)
                     /gene="ddlB"
                     /locus_tag="ACHU0J_001217"
     CDS             complement(18267..19187)
                     /gene="ddlB"
                     /locus_tag="ACHU0J_001217"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1215977.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19180..20655)
                     /gene="murC"
                     /locus_tag="ACHU0J_001218"
     CDS             complement(19180..20655)
                     /gene="murC"
                     /locus_tag="ACHU0J_001218"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1215978.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20709..21776)
                     /gene="murG"
                     /locus_tag="ACHU0J_001219"
     CDS             complement(20709..21776)
                     /gene="murG"
                     /locus_tag="ACHU0J_001219"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1215979.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21773..23017)
                     /gene="ftsW"
                     /locus_tag="ACHU0J_001220"
     CDS             complement(21773..23017)
                     /gene="ftsW"
                     /locus_tag="ACHU0J_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1215980.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23017..24333)
                     /gene="murD"
                     /locus_tag="ACHU0J_001221"
     CDS             complement(23017..24333)
                     /gene="murD"
                     /locus_tag="ACHU0J_001221"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1215981.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24336..25418)
                     /gene="mraY"
                     /locus_tag="ACHU0J_001222"
     CDS             complement(24336..25418)
                     /gene="mraY"
                     /locus_tag="ACHU0J_001222"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1215982.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25412..26770)
                     /gene="murF"
                     /locus_tag="ACHU0J_001223"
     CDS             complement(25412..26770)
                     /gene="murF"
                     /locus_tag="ACHU0J_001223"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1215983.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26767..28254)
                     /gene="murE"
                     /locus_tag="ACHU0J_001224"
     CDS             complement(26767..28254)
                     /gene="murE"
                     /locus_tag="ACHU0J_001224"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1215984.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(28241..30007)
                     /gene="ftsI"
                     /locus_tag="ACHU0J_001225"
     CDS             complement(28241..30007)
                     /gene="ftsI"
                     /locus_tag="ACHU0J_001225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1215985.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30023..30388)
                     /gene="ftsL"
                     /locus_tag="ACHU0J_001226"
     CDS             complement(30023..30388)
                     /gene="ftsL"
                     /locus_tag="ACHU0J_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1215986.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30385..31326)
                     /gene="rsmH"
                     /locus_tag="ACHU0J_001227"
     CDS             complement(30385..31326)
                     /gene="rsmH"
                     /locus_tag="ACHU0J_001227"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1215987.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31328..31786)
                     /gene="mraZ"
                     /locus_tag="ACHU0J_001228"
     CDS             complement(31328..31786)
                     /gene="mraZ"
                     /locus_tag="ACHU0J_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1215988.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7704 a         9079 c         7952 g         7590 t
ORIGIN      
        1 gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc
       61 gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat
      121 tcaaacctcc ttgatgtaaa caaatcaata caacaaacgg aattgcaaac ttacacacgc
      181 atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt tcatgatgga aggtcaacag
      241 cacggcgagc agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt
      301 ggcgcgatag ggacagggct attcctaggt agcgcctccg taatacagtc cgcagggcca
      361 gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg
      421 ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac
      481 tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt
      541 gccatggcag agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatccca
      601 acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt
      661 aaagtgtttg gcgagatgga gttctggttt gccattatca aagttattgc ggtagtagcg
      721 atgatcatct tcggcggctg gctgctgttc agtggcaacg gcggcccgca ggcgagcgtt
      781 agcaacctgt gggatcaagg tggtttcctg ccgcacggct tcaccggact ggtgatgatg
      841 atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa
      901 gctgataacc cggagcaaag tatcccgaaa gcaactaacc aggttatcta ccgcatcctg
      961 attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc
     1021 gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat
     1081 gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc
     1141 aacagccgta tgctgtttgg tctggcacaa caggggaacg cgccaaaagc gctggcgtct
     1201 gtcgataaac gcggcgtacc ggttaacacc attctggtgt ctgcgctggt tacagcattg
     1261 tgcgtattga ttaactatct tgctccggaa tccgcatttg gcctgttaat ggcactggtg
     1321 gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg cgcatatgaa gttccgtcgc
     1381 gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac
     1441 tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg
     1501 gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt
     1561 aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca
     1621 gggtgagggc aataatctct acaccaacgc cccgtaaatc gtcagtagcg caatcaccac
     1681 cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt
     1741 atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaata cctgatattg
     1801 cgaagtatgg aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc
     1861 gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac
     1921 tgcatcaatg ccggactgta aacgatgatg cggcgtctgg tatcgcgcca gccagtattg
     1981 ccatgcacgc aagaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat
     2041 cagccagaac agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag
     2101 ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc
     2161 gccagccatc gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg
     2221 ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag
     2281 cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacacc
     2341 catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa
     2401 gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa
     2461 aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcgcacga
     2521 aaccgtgccc aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa
     2581 tggcccgtca tgttattagc gatatcggga tagcgatcta tcagtgcccg cgtaaccgcc
     2641 gcaagctgtt gatactgcgc atcggtatac gccagagtat cggtgccttc aagctcaatc
     2701 ccaatagaaa aatcattgca gcgttcacgc ccctgatact gagagactcc cgcatgccag
     2761 gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga
     2821 gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt
     2881 ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg
     2941 tgcaccacca gcagggtggg tgtttcgtca tccaggcggc aatcgtaatg tggtgaggga
     3001 acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt
     3061 ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga
     3121 gttttaacat gccgcctcgc cgctacaacc ctgacacccg acgtgacgag ctgctggaac
     3181 gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa
     3241 cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaattct cgctctcatg
     3301 ccacggtgat cacccgcgag aatggcgtat tttgtggcaa acgctgggtt gaagaggtgt
     3361 ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca
     3421 tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg
     3481 aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact
     3541 atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg
     3601 gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg
     3661 ggctttctga tgccttcctg atcaaagaaa accatattat tgtctccggc tcagtgcgcc
     3721 aggcggtcga aaaagcgtcc tggctgcatc cggatgcgcc agtagaagtc gaagtagaga
     3781 atctggaaga acttgatgag gccctgaaag ccggagccga tatcatcatg ctggataact
     3841 tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag
     3901 tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta
     3961 tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct
     4021 aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc
     4081 ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaatc
     4141 tgctttcccg tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcac
     4201 caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg
     4261 atggtggtta ttggcatcat tgccatttta agctccattg gtattcccgc ttatcaaaac
     4321 tacctgcgca aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc
     4381 gtagagttgt gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc
     4441 attccctcgc ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg
     4501 tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg tcatgacgcc aggttgggat
     4561 aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata ttcaaagtga cagcgcattg
     4621 cagcaagcct gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat
     4681 attccacagc tcacggccct gtgcctgcgt tatcagggag tcttgctgga tgccagcgaa
     4741 gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg agttgctgga cgcattgcat
     4801 ttcgctacca ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac
     4861 gccagtcgca cacaacagac attacccgta gctgttcagg agaagcatca gcccaaagca
     4921 gagttgctga ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc
     4981 gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta
     5041 ccggatgttt caccggatgc cggagtcgca ttaacagcca gattaaaagt gctgggaaac
     5101 ctggatattg cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga
     5161 aacaccgtct catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta
     5221 aggttgttac agcaggtgag ccaggcactg gatgttaaca cgctgggaat gcagccgtta
     5281 caactggcgg actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc
     5341 cctacaggca gtggcaaaac ggtcacctgg tgttgtccac gctacacacc aattccacca
     5401 ctgaaacgct ggtacgttta cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc
     5461 ttacgctggt aatagcccag cgtctggtac gtaaactttg cccacattgt cgccgacagc
     5521 aaggggagcc catccatatt ccagacactg tatggccgtc gccgctgccc cactggcagg
     5581 cacccggttg tgtacattgc taccacggtt tttatggtcg taccgccttg tttgaagtcc
     5641 tgcccattac accagccata cgccagctta tttccgctaa tactgaagtt gaatcgctgg
     5701 aaacgcacgc ccgacaggcg ggtatgcgta cgctttttga aaacggctgc ctggccgtgg
     5761 agcaaggctt aaccaccttt gaagagttaa tccgcgtact ggggatgccg catggcaagt
     5821 aagcaactct ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta
     5881 tgggcagaga gccgtacttt actgcttatg gcactacagc aacagatggt taccccacta
     5941 agcctgaagc gaatcgccat caattctgcg cagtggcgag gagataaaag cgcggaagtc
     6001 attcatcaac tggcgacgct acttaaagcc gggttaacgc tttctgaagg gctggctctg
     6061 ctggcggaac agcatcccag taagcaatgg caagcgttgc tgcaatcgct ggcgcacgat
     6121 ctcgaacagg gcattgcttt ttccaatgcc ttattaccct ggtcagaggc atttccgccg
     6181 ctctatcagg cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt
     6241 gaactggcgc gtcagcaaaa atcccagcgt cagttgaccg acaaagtgaa atcagcgttg
     6301 cgttatccca tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt
     6361 gttctgccgg agtttgccgc tatctataag accttcaaca ccccactacc ggcactaacg
     6421 caggggatca tgacgctggc agactttagt ggcgaatggg gctggctgct ggtgttgttc
     6481 ggctttctgc tggcgatagc caataagttg ctgatgcgcc gcccgacctg gcttatcgcg
     6541 cggcagaaat tgctgttacg catcccgatt atgggttcac tgatgagggg acaaaaactc
     6601 acgcagatct ttacgattct ggcgctgaca caaagtgcag gcattacttt tttacagggc
     6661 gtagagagcg tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc
     6721 cagcacgata tcagtaacgg tcatcccatc tggctggcgc taaaaaatgc cggtgagttt
     6781 agcccgctct gtttgcaatt agtgagaaca ggagaggcat ccggctcgct ggacctcatg
     6841 ttagacaacc tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctcgca
     6901 gccttactgg aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg
     6961 gcaatgtatc tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg
     7021 cgtgttgctc cacgccagcg ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt
     7081 ccagcacacg aataaacgtg gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt
     7141 atgtacaagc tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca acctggcctc
     7201 gcagcggcag cttaacggtt ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt
     7261 gacgtttcat cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct
     7321 cctcaacgat gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga
     7381 aatcggcacc gccgccaaag gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc
     7441 tgacgatcat tccgcccaga ccgtgcgccg catcggcaca ttcgattacc gcagaaagct
     7501 gcggataacc gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa
     7561 ctttaacgat atcagcaccg gagaggataa gctcctcaca catttcacca gtcactacgt
     7621 tgccagcaca aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga
     7681 agtgttcgga ataaccattc gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt
     7741 cgagaatctg tttagttttt tcgaaatccg catcagacgt gccggtagaa accatcacat
     7801 gtttcagcac atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaaaagt
     7861 gtttatgcac agcagtcaga atatcaaaag aagccagcgc agaggccata gaaaatgtgc
     7921 ctacggtgtc catatttgcg gcgataatcg gcacgccgga ccagctctga cctgaatgtt
     7981 tgaaggtgaa ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag
     8041 ggcggatgag aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat
     8101 tcctggggtt aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact
     8161 aatcaatgcg gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta
     8221 cctggtaaat agtgagcgaa cgtttcatca atttcacttt cgcgcgctca tttttaacgt
     8281 tttgataatc atgatgaatt cccgggaata taaattatgg ggtatatagt tgccttaacg
     8341 ggaggcattg gcagtggtaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac
     8401 gtcattgatg ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat
     8461 gccattgctg atcactttgg cgaaaacatg attgctgctg atggaacatt gcagcgccgg
     8521 gccttgcgcg agcggatctt cgccaacccg gaagagaaaa actggcttaa cgacctgctg
     8581 catccgctga ttcaacaaga gacgcaacac cagatccagc aagcaacttc cccctatgta
     8641 ctgtgggttg tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg
     8701 gtggtggatg tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact
     8761 cgcgaacatg tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg
     8821 gcagatgacg tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc
     8881 ctgcacgcac actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc
     8941 agacccaggt cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg
     9001 agtttttgat tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc
     9061 atttcttccg taatgtcagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg
     9121 agctgttgaa agaacttaac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg
     9181 gcgtggacca gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gtgagcgtat
     9241 taatttccgc gccgcgtatc gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc
     9301 gtcagcgact gagcatccca ggcggctgtt gcagctttga tttacctaca ttgcacattt
     9361 ggctgcatct accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga
     9421 acccgctcac ccaggcactt accatggtgc tggatttaat tcgccagtcg gcccccttcc
     9481 gtaaacaaac cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc
     9541 gcctgaatct gtcgctcgat tcacagcttt atccgcaaat ttccggacat aagagccgtt
     9601 ttgccattcg ttttatgccg ctggacactg aaaacggaca ggtaccggaa cgtctggatt
     9661 tcgaactggc ctgttgctaa ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa
     9721 cctgcgggaa aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac
     9781 gttgtcagct gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg
     9841 gcgatctttc cgaaagcgat gactggagcg aagagccaaa gcagtgacat ttgtctgatg
     9901 cctcacgtag gcctgataag acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg
     9961 gatgcggcgt gaacgcctta tccgacctac agacgcttaa gcttcgcaat taccggttca
    10021 ttggctggcg gaaaatcatc ggcattaaga ccgaccagcg acatccactc accgggttgc
    10081 ccttctttac cccacggccc cccttcccaa ctttcgacca gccagaacca cagagttata
    10141 tgcctgtccg ggaattcata ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc
    10201 ccgacctctt cctgaagttc acgcaccacc gcctgttccg gcgtttcgcc catttcaatt
    10261 ttaccgccgg gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata
    10321 aagatttcat tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtc
    10381 ttaaacctat aaaaaaggcg cagaatcctg cgccttttac ttcaactgtt agcttttatt
    10441 gcaggcgacc atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcgttac
    10501 gtcctacttt gcgttcaccg gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat
    10561 cctgatggct aagctgctgc atttgtgcta aacgctcggc ttccatacga cgctgttgtt
    10621 ccagctcctc aacctcttca ggcatacgta cctgaacttt gctcagcgta ctgataactt
    10681 catatttcaa cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct
    10741 gcttcggatc tttctgtgca tagccacgca ggtggatacc ctgacgcaga tagtccatcg
    10801 ctgccaggtg ctctttccac agggagtcaa gcgtttgcag catgacgcct ttctcgaagt
    10861 gacgcatcat ctcagcacca accacttctt ctttacgctg atacacttcg atggactgcg
    10921 ccagaatgcg ctcacgcagc gtctcttcat gcagttctgg ttctttatcc agccactcgg
    10981 taattggtaa atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc
    11041 acatttcttc cagcgactgt ggcggaatgt aggcatcaat ggtggctttg aacacatctt
    11101 cacgaatgct gttgatggtt tcgctcacat cgctgacatc cagcagttcg ttacgctggg
    11161 agtaaatggc gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa
    11221 tgtcgaagtt acgactttca actttacgct gggcgttggc aatcgcttta gtcacccacg
    11281 ggtgttcaat ggcttcgcct ggcttcatac ccagtttacg catcatgccg gatactcggt
    11341 cggaagcaaa aatacgcatc agcgcatctt ccatcgacag gtagaaacgg gaagaaccag
    11401 catccccctg acgaccagaa cgaccgcgca actggttatc gatacgacgg gattcgtgac
    11461 gctcggtacc gatgatatgc aggccacctg cttccagtac cgcatcgtga cgtacctgcc
    11521 agtcggcttt aattttttca atttgctctg cggtcggatt ttccagcgcg gcaacttctg
    11581 cctgccagct acctccgagc acaatatctg taccacgacc cgccatgttg gtcgcgatag
    11641 tcaccgcagc cggataacct gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg
    11701 cgttcaggac attgtgctta ataccggctt tggtcagttc gtttgacacc agctccgatt
    11761 tttcgatgga gatagtaccc accagcaccg gctggccttt cgcagtacgt tctttgatat
    11821 cttcaatgat cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc ggcagatctt
    11881 tacgaatcat tggacggttg gtcggaacaa cgacggtatc cagtttgtag atggagctaa
    11941 attcgaaagc ttcggtatca gcagtaccgg tcatccccgc cagtttttca tacagacgga
    12001 agtagttctg gaaggtgatc gaagccagcg tttggttttc gttctggatc tgcacacctt
    12061 ctttcgcttc cacagcctgg tgcagaccat cggaccagcg tcggccctgc atggtacgac
    12121 cggtgtgttc gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtcacggg
    12181 taaacagcgc atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg
    12241 gagagtacag agactcccct tcatccatga tgccctcttt caccagcagt tcttcaatca
    12301 gcaccagacc acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc
    12361 cttcgccctg gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt
    12421 tattcacgcg tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg
    12481 tacgcgcttc atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac
    12541 gctgtacacg ttcttcaggg ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt
    12601 cgttgttcgt accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca
    12661 tgcccggcag gttgatacca acagtcaggc caaggaattc aaacagcgga cggttgtttt
    12721 cggcgtcacg ttgcgccagg tagtcgttga cggtaactac gtgcacgcct ttaccggtta
    12781 gtgcgttcag gtaagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt
    12841 cggcgatgca gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca
    12901 taccaaagac gcgcttactt gcctcacgca ccacggcgaa agcttccggg atcagatttt
    12961 ccagcacttc gcctttttcc agacgcgcac gaaactctgc ggttttccct ttcagttctt
    13021 catcggagag tttttccatc tccggttcca tggcattgat gatgttgacc actttgcgca
    13081 tccggcgcag ggtgcgatcg ttacgactac cgaaaacttt agttaacaat ttgattagca
    13141 taataaaatc tcaaacgccc cgcgttacgg agttaataaa atgaagtaaa ggtttattgt
    13201 tgttaggtga ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc
    13261 gtgctgaatt ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac
    13321 ggcgtgcctt cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc
    13381 tgaagaggca aagattcttc agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga
    13441 tgacggatta ccgtacgaat agcatgttga tgccagtaat caacggaata gttcggattc
    13501 gggcggcgcg tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc
    13561 tcgtggttgc gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca
    13621 ggcaaaccta aactcgccgc gaccatccct aataagagat gcggccagaa gtagcgttta
    13681 ccaaactgtc gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgaattca
    13741 aacgtattta gcgcacaaat cttatcatta aagcctgaag tccacagcac gaatgaagat
    13801 aagttgtgcg taaaagtgct taagaaaaca gcgttcgcac cgttttttat agctcttata
    13861 aaattcaaaa aaaaacgact ggttcacctg gccggagaga gtgccagatt tgccagtcga
    13921 attttatacg acagtataaa tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca
    13981 acggcagttc tgcgtcgtcc tggaaggtca catattccca ggcttcctgt ttcgccagga
    14041 cagcctgcag cagtttgtta ttcagtgcat gaccggattt ataagcggta aatgcaccaa
    14101 taatattgtg accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa
    14161 attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg
    14221 cacaatcgaa gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca
    14281 tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca
    14341 tcgcatagcg ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca
    14401 gcgaaaaacc attgtacggc ttaaattcag cccacttatc gccatcttcg acacgaacag
    14461 tctctttgat gcgaacaaat tttttggcgc agttcaactc gtcgataccg gcgtcaagca
    14521 gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg gatttccggc gcgttaactt
    14581 cgataacaat gttatcgatg cccaggcccg cgagagcagc attgaggtgc tctacggttg
    14641 aaatccgtac atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt
    14701 tggcatcggc cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg
    14761 tgttggccgg cgcagggcgt aacgtcaggg tgactttctt gccggtatgt aaaccgacac
    14821 ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa
    14881 ttacctatcc aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc
    14941 gaaacccaaa ttccagtcaa ttcttaatca gcttgcttac gtaggaatgc tgggatatcc
    15001 agataatccg gctctttcgc agtttgcggc gcattgtcat tcactacttt agcaaccggc
    15061 ttctgctcct gggtcagcgg agccatccca tgctgctggt agcgatccat cactggctgc
    15121 tgaacctgct tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc
    15181 gcaacaacgg ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc
    15241 acagtcgcgt tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc
    15301 agacgcaggt cgaagcccgc cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg
    15361 atatcttcca gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca
    15421 ccgctcgcca cgccagaacc catcattgcg tagcccattt cagacattac ggtgcgtacg
    15481 tctgcaaagt ccacgttcat caaacccgga cgagtaatca gttcggcgat accttgcaca
    15541 gcgcctttca gtacatcgtt cgctgcgcca aacgcatcca gcagggagat accgcggccc
    15601 agaactttca gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt
    15661 tcagtgatcc cctgctcggc gaatgccata cgcttcttgc cttcaaagtt gaaaggctta
    15721 gtgacgacag caacggtcag gatacccaaa tcttttgcca cttcagcgac gactggtgct
    15781 gcacctgtac cggtaccacc acccataccc gcagcaataa agaccatgtc tgcaccttcc
    15841 agcgccgcac gcaatgcatc gcgatcctca tcagccgcat tgcggccaac ttctggatta
    15901 gcgccagcgc ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct
    15961 gttttacgca gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatacgc
    16021 tcgcgcacca tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc
    16081 accgcgtcat tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc
    16141 gcctgagtcc gtaatcatcg tcggcctcat aaaaattaaa actcttttcg cagccaacta
    16201 ttgagtcgct tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca
    16261 ccgttaagat gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc
    16321 tcctgagcat aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta
    16381 tgaaacacgc gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accggttaat
    16441 acaatgcctg ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc
    16501 aatatctctt cgttgaccag gttgagcaac tcggtatagc gcggctcgat cacctctgcc
    16561 agggtctgac gttgcagact ccgcggcgga cgaccgccta cgctcggcac ttccacgctc
    16621 tcatcttttc caacgatgga acccagcgca caaccgtggc gaactttaat cgcttcggcg
    16681 tcgcttggcg gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt gccagcataa
    16741 ggaattacct ttgtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca
    16801 ccaccgatat cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa
    16861 cttgatgcca gtccggcaaa tatcagttgg tcaactttca gcccacaacg ttcaaccgct
    16921 ttgacgatgt ttttcgccat atcgttgtga catgtgatca ggtgcacttt tgcctgcatc
    16981 cgcacacccg aaagtcctac cggattcttg atcccttcct gatagtcaat cgcatactct
    17041 tgcgggatca catgcagcac acgatgctca tcgcgcacac gcaccgattt cgcggtatgg
    17101 acgacgtttt ccacatcttc ttgcgtcact tcttcttcag aaataggcac cataccaatt
    17161 tcattctggc agctgatgtg cttaccagaa agcgccagat ataccgaaga gatctgacaa
    17221 tctgccatca attctgcctg gtcaatggcg cgttgtacgc acttgaccac ggattcgagg
    17281 tcgttcaccc cgcctttatc cataccacgt gacgggcagc tgcccacgcc aatgatattg
    17341 accataccgt cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatttcc
    17401 agtcctacta ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat
    17461 tttgttgctg agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt
    17521 cataacgcaa atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa
    17581 ccggataaag ttctacaaag cgaaccaaac gtttcatcgt atcgccccgg ccaagattga
    17641 gcttaatatc gttattcagc gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct
    17701 tcagagtaaa tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt
    17761 cattagcgct gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg
    17821 gcggcacgct gaaggtgttt ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa
    17881 tcggcacata ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca
    17941 cctgcttaat ccacggcagg cgttgttcta tttgcgtctg gatgatgttg acatcctggg
    18001 tcataaaggt acccggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg
    18061 tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca
    18121 tccagctcaa cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa
    18181 ggatccccgc cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcgctgtttc
    18241 gcgtgttcag agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac
    18301 tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg gctggtcata
    18361 cccggtgagg tattggcttc cagcagataa aactgtccat cactgtccag cataacgtca
    18421 atacgtcccc atcctttgca acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa
    18481 ttggcctctt gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga
    18541 tacttcgcct cataatcata gaaggttccg gacggttgaa tacgtattga cggtaaaatt
    18601 tcttcaccga gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact
    18661 tcttcatcgt gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact
    18721 tttgacattc ccacactgga accttcgcgg ctcggcttaa cgataaccgg caaacccaga
    18781 gcagaaattt ctgctaactg cttatcgctc aggccttttt caaactctgc gcgggttaac
    18841 gctacccacg gcgcgaccgg taaaccggca ccttgccaca gaagtttgct gcgtagttta
    18901 tctattgaaa gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctcg
    18961 agcatgccct gcagcgtacc atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc
    19021 tgaaaaccca tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca
    19081 tcaataccgc cttcacgcag cccggctaac accgctgcgc cagaattcag agaaacttcc
    19141 cgctcagcgg aagtcccgcc caacaggacc gcgattttat cagtcatgtt gttcttcctc
    19201 cggagtttgc ggcttcagtt tgatttcagc taaagaacgg gcaatttttc cgatattacc
    19261 agccccctga acgagaatca ggtcgttacc ggttaatacc ggtgccagca tctcggctac
    19321 ctgcgcaggg tccggcacca gaatgggatc aattttccca cgtccacgaa ttgtgcgaca
    19381 cagcgaacgg ctgtccgctc ccggaattgg cgcttcgcca gccggataca cttccagcat
    19441 caacagggta tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg
    19501 ggtaaaacgg tgcggctgaa acagcattac caggttttta tccggccagc ctgcgcgcgc
    19561 cgctttaatg gtggcgtcaa cttccgtcgg gtggtggccg tagtcatcga ccagcattgc
    19621 cgtaccgctt ttaccattca ctggctccag cgggaattca ccgaggaaat caaaacggcg
    19681 accagtcccc tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc
    19741 cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag
    19801 ggtgacgcac atcggctctt tgtcctgacg cagcagcgta aagtgcccct gcgggccaat
    19861 ctgctgataa tcttctacac gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg
    19921 acgtcccaca cgcggtaaca attcgcggat caccggatca tcaacacaca tcaccgcacg
    19981 accgtaaaaa ggcaggttgt gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc
    20041 gccctggtag gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg
    20101 cagatgcagg aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc
    20161 caaacgcgca tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag
    20221 ccccgcttct gcgtagatgc tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc
    20281 tgcaatggcg atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat
    20341 caccggaata cgcgcttcat gagctgcgac aatttccggg ttgtcggcag aaatggcgct
    20401 ggaaacaacg accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt
    20461 cgcacccaga ttcagtaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat
    20521 ctgataacct tcattggcca gaacttcggc aataccgccc ataccggcac caccaatgcc
    20581 gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa
    20641 ttgttgtgta ttcattcttt acgccattaa cttcttaaat tcatacgatg caaaaggcat
    20701 cgctacaatt acgcccgggc agcccggctc acttcatttg ccactcgctc ggtggcatcc
    20761 ggaatggatg cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg
    20821 gccagggtgt tggcgacagc atccacgcta agctgtggct gctcgataat tttggctgcg
    20881 cccgcttttt ccagcggtag cgcattccag tattgctggc ggtctttatg ttgaaacggc
    20941 acaaacaacg ccggtagtcc tgccgcggcg atttcactca cagttaaagc accggagcgg
    21001 caaacgacga catccgccca cgcatacgcc gccgccatat catcaataaa ttcagtcact
    21061 ttatgctgcg gttgccccgc ttcggcatac gcctgttcaa cggattgttg cgaacctttg
    21121 ccgctctgat gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc
    21181 tggttaagaa tgcgtgcgcc ctgggaacca cccactacca acacacgaac cggaccttca
    21241 cgtccagcca aacgttgctg cggcaacggc agcgccagca catcggtacg caccgggtta
    21301 cccaccactt ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc
    21361 ttcgccagcc atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga
    21421 atgcctaacg accacgcggc cagaccacct ggacctgata catagccgcc cataccgagt
    21481 accacgtcag gtttgtacgc tttcataatc gcccgcgcct gacgccaggc gttgaagata
    21541 cgcagcggcg cagctatcag tgcttttata ccttttccac gcaaaccaga gatacgaatg
    21601 aaatcaattt cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc
    21661 agccagcgaa cttgccaacc ctgagccatt agatagtgcg caaccgccag tcccgggaat
    21721 acatgtccac cggttccgcc tgccatcacc attaatcgct ttccttgacc actcatcgtg
    21781 aacctcgtac aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca
    21841 tcatgatggc tgtcgacata atcagtaagc tcgaaccacc gtaactgatt agcggcaatg
    21901 tcagaccttt ggtcggtaac atccccgccg ccgcgcctac gttaaccagc gcctggaagc
    21961 taaaccagat gccaatagaa caggcgagaa aaccggaaaa acggtggtca atttctaatg
    22021 ctttgcggcc aatcgacatc gcacgaaaag cgacgaagaa taccattaaa agtgctagta
    22081 ccacaccgac ataacctaat tcttcgccga taatggcgaa aataaagtca gtgtgcgctt
    22141 ccggcagata ctccagtttt tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc
    22201 gaccaaacgc catcagcgat tgcgttaact gatagccgct gccaaaggga tcttcccacg
    22261 ggttccagaa tgcggtaaca cggcggatac ggtacggttc ggcgaggatc agcaacacaa
    22321 ccgctgaaat gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga
    22381 acaacatcgc cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca
    22441 gcagtaacac tgccaacacc agaatcacgc ccatcggttt caggaagccg cgcaggttat
    22501 tacgtacttc gtcgccttta cgcaccagat agttggcgat atagcaaaac agcgacagtt
    22561 ttgtcagctc cgcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt
    22621 taacagcgct acccactacc agcacgatca tcagcaggat gatagatccg agcagcatcg
    22681 tggcactgta gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaataa
    22741 acgccaaaat cagatagaca ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc
    22801 gttgccctat gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcgaggccga
    22861 aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg
    22921 agcccatcac ccagcccttt agcgccgtgg agatccagac caggatactg aatcctggca
    22981 ggcgcggcat tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg
    23041 ggcaaactca ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg cacaggccgg
    23101 ggagagcaga accatatcgc ccggctgaac acgcggagcc agcaagcgca tagcctgttc
    23161 catagtttcg gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg
    23221 accgaaacaa tacagacgta cgttatcgcc attcaggtaa cgcaccagtg ggctaaagtc
    23281 cgccgattta ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt
    23341 cagcgccgct tccgtactgc cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc
    23401 gttatgctcc agcaccactt caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt
    23461 caggctgctg gcgcgcggta accctgcggc atctgccagc gccagcgcca ccagcgcatt
    23521 ggtgtagtta tgctgcccgg aaagtttcat ctctttcaca ttcagcactt tctcgccttt
    23581 aacccgcagc caggtttcgc cctgctgatg attcaggtga tagtcaccca tgttgacgcc
    23641 aaagctgacg cagcgttcat ccgcaccgcg aatcggcatt gttaaggcat catcagcatt
    23701 aaccacgcaa actttcgcgt tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa
    23761 accaaacggg tagcgatcca tatgatcttc agtcacgttc agaatggtcg ctgctaccgc
    23821 ctgtaagctg gaggtggttt ccagctggaa gctcgacagt tccagcacgt acagttcaca
    23881 ctcatcatcc agtagcatca acgcaggcag gccaatattg ccacccacac caacgttaac
    23941 ccccgccgct ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc
    24001 ggtaatcgcc acaatcggtg cttgtgcttc gcgacaaaac agctcgatat cgccaacgat
    24061 ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa taccgggact
    24121 ggcgacaatc agatctgccg ccatcagcca ttcatcattc agactgcccg tgtggcgttc
    24181 tacggcttcg ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg
    24241 cgtcacaccg cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat
    24301 aatgacaaca tttttaccct ggtaatcagc catgattaac gtaccttcag cgttgccaga
    24361 ccaatcagga ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc
    24421 cagcctttca gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc
    24481 agtttaaagg agccgacctg caggatgaca gaaagcgttt ctaccacgaa cacgcccccc
    24541 ataatcacca gcaggaattc ctgacgtagc agtacggcga taatgcctaa cgcaccacct
    24601 aacgccagcg aaccgacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg
    24661 aagcccagtc ctgccccgac tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc
    24721 agatacggta tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca cgccaccagc
    24781 gcaaaaccac cggcgacaaa tacggtcggc ataattgcca gaccgtcaag accatcggtc
    24841 aggtttaccg cgttgccagt acccacaatg acgaagtaag ccagcagaat gtagaacagc
    24901 cccagttgcg gcatcacatc tttaaagaat gggaccacca gctgcgttgc gggcgtgtct
    24961 ttgccggcaa ggtacaaagc gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat
    25021 ttccaacgag cgatcaaccc tttggtgtct ttacgcacca ctttgcgata atcatcaaca
    25081 aagccaataa caccgtaacc taccagcacc accaacacgc accagacgta cggattggac
    25141 gggtaagccc acagcagcac ggagatcaca atcgctgtca ggatcataat cccgcccatg
    25201 gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac tacctgacca
    25261 aaggaaagtt tttgcaaatg agcaatcatg cgcgggccca tccacaatga gatgaacagc
    25321 gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa
    25381 taatatttga ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaaagcgc
    25441 gtactacctc ttccatggca gcactacgtg aacccttaac taaaatcgta attacctgtt
    25501 gctcagcaat cagtgattta agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc
    25561 caacgccgct ggcggtgctg atagcatggc tttgtttacc catgcttaac acgcggtcaa
    25621 taccagcagc ttttgccgcc tcacccacct gtacatggca ggcttcgctt tcagcgccca
    25681 gttccgccat atcgcccacc accagcacgc ggtagcccgg catttcagcc agtacctgaa
    25741 cagctgcggt cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt
    25801 ctgccagttg gatggggaac agacggccag gaacagcttt cagatttgcc agccccgctt
    25861 tgatagcatc aagcgttgcg cccacggaca tggagagcgc agcggctgcc agcgcattcg
    25921 caatattgtg acgccccggc aacggcagca gaacatcgac gctaccggtt ggggtttgta
    25981 gggtaaattc cataccgtgc gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg
    26041 cggcattggg tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcagcc
    26101 agtcgttgtt gtcggcgttc ataatggcga taccgttttc cggcaggccg ctaaagattt
    26161 cacctttcgc tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc gccgctgcca
    26221 ggttgttgac cagcgccgct tccgggcgag tcagactcac agtccaggca atctcgccct
    26281 gatggttcgc gccaagttca ataactgcgt aatcgtattc cggcgttaag cgcaacagcg
    26341 tcatcggtac accgatgtcg ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact
    26401 ggcttaaaat cgccgccgtc atctctttaa cggaggtttt gccggaggac cccgtcagag
    26461 caaccacgag cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac
    26521 gcgtatcctt gacgattaac tgcggcaggt cgatgtccag cggacggcta accagtagtg
    26581 cgcctgcgcc gccagctttc gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt
    26641 tcagggcaac aaacaggcag cccggcgtca gttttcgggt atcagtggtt acagcatcaa
    26701 gggtgatatc tgcaccttgc agttcaccgt tgagaatatc ggtaagttgg ctaagggtta
    26761 cgctaatcat gcaatccccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag
    26821 acgctgattg ccaacaatct ggtaatcctc atggcctttg cccgcgacca gtaccacatc
    26881 attctcttta gcctgcataa cggcgcaagt caccgcttca gcacggcctt ccatcacttt
    26941 ggcatgtccg gcatctaaca ttcccgccag aatatcgttg atgatggcac gcggttcttc
    27001 ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagca aactcttcgg caattgcgcc
    27061 catcagtgga cgcttacctt tatcgcgatc gccaccacag ccaaagacgc accacagctt
    27121 gcccgcacag tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc
    27181 gtaatccacc accaccgtcg gtttgcctgg cgcagtgaac acttccatac gtccgcaaac
    27241 cggttgcaga cgcgcggcgg ttttcagcag atcagccagt ggatagccca gcgccagcag
    27301 tgttgccagc gcgagcaaca ggttgctgac gttaaaagcg cccatcagat ggctttcaat
    27361 ttcgccatcg ccccagcttg aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac
    27421 ttcggtcgct ttcaaccagc gtccgtgaca gttcggattg atatgatctt ccattgatac
    27481 cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc acttcatcgt cggcgttaat
    27541 aatcgcctga ccgcaatgat gttcagaata aagcagccat ttcgcagctt cgtagtgttc
    27601 catatcacca tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa
    27661 tttcaatgcc gccacacggt gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa
    27721 agtcgcgccc tgatccacca gccccgccag ctcatgctga acatcaactg ccgaaccggt
    27781 agtattttct gtcgggatca ctttccccag cagaccgtta ccaacggtgc ccattaccgc
    27841 gctggtttcg ccaagcagtt ggctccactg cgccagaagc tgggtcgtcg tggttttgcc
    27901 gttggtgccc gttacgccta cgagacgtaa attgtcagag ggttcatggt aaaagcggcc
    27961 cgccagtgca gataaacgct cgttgagctg gctgagatag atgaccggta cgccgtgcat
    28021 ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg cagccacacc
    28081 ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac
    28141 aaagagatcg cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga
    28201 aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc
    28261 cctcgccttg attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga
    28321 tgttcatggt acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc
    28381 cgccgtagta tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct
    28441 gactcgcagg cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg
    28501 gcccgacctt tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct
    28561 tcacgccgcc gccgcctggt agcgccacgc tttccatcat atgcaccacg gtgcgaacaa
    28621 tggattccgg gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg
    28681 ggcgataaat gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta
    28741 ccattagccc gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt
    28801 gaggatataa gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa
    28861 aacgtgagta agtatctact aacgctgagg acggcatcgt taacgccagc ttggaaacac
    28921 cgacgttact cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt
    28981 ctttgatttc gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca
    29041 ccacgccacg ttgcaacgcg gtcatcacca ccatcggttt aaccgttgag cccggttcaa
    29101 acacgtcggt gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag
    29161 ggttgtatga cgggctgtta gccatcgcca gcacttcacc tgtgttgaca tccaccagca
    29221 cggcgctacc agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca
    29281 gcgcctgcag gcgttcatca atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag
    29341 aagaaatatc ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct
    29401 gcccggtaag ccatttatcg aaactcttct caacgccctc aatcccttga ctatcgacgt
    29461 tagtaaagcc gatgaggtga gcagtcactt cgccggacgg atagtaacgg cgagactctt
    29521 cacgcagatg aatccccggc agtttcagtt ttttgatgta gtccgccatg tcagagttta
    29581 cctgacgcgc cagataaata aagcgccctt tcgggttggc gttaatgcgg gcagaaagct
    29641 gatccagcgg aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac
    29701 cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg
    29761 ctaacgggcg accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc
    29821 gaagagaacg catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg
    29881 ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac
    29941 gccaactgat aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg
    30001 ctttcatgcg tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa
    30061 catgctgcat ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg
    30121 cattctcttc aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca
    30181 gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acggtcaccg
    30241 ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt
    30301 caccgataac accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc
    30361 ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc tctgcaatac gcagaactga
    30421 actacgggca cgagggttct cagccacctc ttcttcgccc ggcattaact tgcctagtgc
    30481 tcgcagctga cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg
    30541 aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga
    30601 gtggaagctg atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag
    30661 cgcctgctct atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg
    30721 ggtcgcggga tgtttaaact tatctttcac cggcgttgca gcagccacga cttccgccag
    30781 ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gggcaatgcg
    30841 tttggcaaaa tgctcttcac cataggtttt caatacccag gcgatatcgg cttcttctgc
    30901 ggtttgtagc cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg
    30961 accatcgcgc ataaaggaaa agccgcgttc agcatcatca agttgcggtg aagagacgcc
    31021 aagatcgagg agaatgccgt cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc
    31081 cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac
    31141 ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga
    31201 gaggatcaga cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg
    31261 acggatattg aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc
    31321 catcatttta tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct
    31381 ctgcgtcgat atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact
    31441 tgttgaactg tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg
    31501 gcgcgattaa caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta
    31561 ggcgctgcac acggcgctca accgggttca tgctcaacag acgcgataat ttttgctcga
    31621 taatttccca ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc
    31681 aaaccatttg accggcagcg ttctcaagca gctgttcccg ataacgggta ggcactgata
    31741 agcgcccttt gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc
    31801 ttattcccac tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc
    31861 ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca
    31921 gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa
    31981 aaaatccctc atcgtcatca ctgttttaaa atgactcact attttccaca gcaaatataa
    32041 agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtcctt
    32101 gttacgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg
    32161 ttaaatcggc aattcatcgc cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc
    32221 acttaattat tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaaccattta
    32281 tcaataatag tcaggaatag tcttatttac tttaaggata ttgat
//
LOCUS       DAZPDE010000028        32005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPDE010000028 DAZPDE010000000
VERSION     DAZPDE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.028"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..69)
                     /locus_tag="ACHU0J_001229"
     CDS             complement(<1..69)
                     /locus_tag="ACHU0J_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1215989.1"
                     /translation="MAMLTGDSHKDVKFMLRMFIPTS"
     gene            complement(122..1012)
                     /gene="rarD"
                     /locus_tag="ACHU0J_001230"
     CDS             complement(122..1012)
                     /gene="rarD"
                     /locus_tag="ACHU0J_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1215990.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1064..1531)
                     /gene="yigI"
                     /locus_tag="ACHU0J_001231"
     CDS             complement(1064..1531)
                     /gene="yigI"
                     /locus_tag="ACHU0J_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1215991.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1696..2565
                     /gene="pldA"
                     /locus_tag="ACHU0J_001232"
     CDS             1696..2565
                     /gene="pldA"
                     /locus_tag="ACHU0J_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1215992.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2698..4527
                     /gene="recQ"
                     /locus_tag="ACHU0J_001233"
     CDS             2698..4527
                     /gene="recQ"
                     /locus_tag="ACHU0J_001233"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1215993.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4591..5211
                     /gene="rhtC"
                     /locus_tag="ACHU0J_001234"
     CDS             4591..5211
                     /gene="rhtC"
                     /locus_tag="ACHU0J_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1215994.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5273..5893)
                     /gene="rhtB"
                     /locus_tag="ACHU0J_001235"
     CDS             complement(5273..5893)
                     /gene="rhtB"
                     /locus_tag="ACHU0J_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1215995.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6004..7026
                     /gene="pldB"
                     /locus_tag="ACHU0J_001236"
     CDS             6004..7026
                     /gene="pldB"
                     /locus_tag="ACHU0J_001236"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1215996.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7034..7834
                     /gene="yigL"
                     /locus_tag="ACHU0J_001237"
     CDS             7034..7834
                     /gene="yigL"
                     /locus_tag="ACHU0J_001237"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1215997.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7910..8809
                     /gene="bioP"
                     /locus_tag="ACHU0J_001238"
     CDS             7910..8809
                     /gene="bioP"
                     /locus_tag="ACHU0J_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1215998.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8697..9650)
                     /gene="metR"
                     /locus_tag="ACHU0J_001239"
     CDS             complement(8697..9650)
                     /gene="metR"
                     /locus_tag="ACHU0J_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1215999.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9886..12147
                     /gene="metE"
                     /locus_tag="ACHU0J_001240"
     CDS             9886..12147
                     /gene="metE"
                     /locus_tag="ACHU0J_001240"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1216000.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12186..13001)
                     /locus_tag="ACHU0J_001241"
     CDS             complement(12186..13001)
                     /locus_tag="ACHU0J_001241"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1216001.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13263..14024
                     /gene="udp"
                     /locus_tag="ACHU0J_001242"
     CDS             13263..14024
                     /gene="udp"
                     /locus_tag="ACHU0J_001242"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1216002.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14165..15592
                     /gene="rmuC"
                     /locus_tag="ACHU0J_001243"
     CDS             14165..15592
                     /gene="rmuC"
                     /locus_tag="ACHU0J_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1216003.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15687..16442
                     /gene="ubiE"
                     /locus_tag="ACHU0J_001244"
     CDS             15687..16442
                     /gene="ubiE"
                     /locus_tag="ACHU0J_001244"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1216004.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16456..17061
                     /gene="ubiJ"
                     /locus_tag="ACHU0J_001245"
     CDS             16456..17061
                     /gene="ubiJ"
                     /locus_tag="ACHU0J_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1216005.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17058..18698
                     /gene="ubiB"
                     /locus_tag="ACHU0J_001246"
     CDS             17058..18698
                     /gene="ubiB"
                     /locus_tag="ACHU0J_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1216006.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18777..19046
                     /gene="tatA"
                     /locus_tag="ACHU0J_001247"
     CDS             18777..19046
                     /gene="tatA"
                     /locus_tag="ACHU0J_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1216007.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19050..19565
                     /gene="tatB"
                     /locus_tag="ACHU0J_001248"
     CDS             19050..19565
                     /gene="tatB"
                     /locus_tag="ACHU0J_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1216008.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19568..20344
                     /gene="tatC"
                     /locus_tag="ACHU0J_001249"
     CDS             19568..20344
                     /gene="tatC"
                     /locus_tag="ACHU0J_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1216009.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20386..21168
                     /gene="tatD"
                     /locus_tag="ACHU0J_001250"
     CDS             20386..21168
                     /gene="tatD"
                     /locus_tag="ACHU0J_001250"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1216010.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21165..21653)
                     /gene="rfaH"
                     /locus_tag="ACHU0J_001251"
     CDS             complement(21165..21653)
                     /gene="rfaH"
                     /locus_tag="ACHU0J_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1216011.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21820..23313
                     /gene="ubiD"
                     /locus_tag="ACHU0J_001252"
     CDS             21820..23313
                     /gene="ubiD"
                     /locus_tag="ACHU0J_001252"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1216012.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23359..24060
                     /gene="fre"
                     /locus_tag="ACHU0J_001253"
     CDS             23359..24060
                     /gene="fre"
                     /locus_tag="ACHU0J_001253"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1216013.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24252..25415)
                     /gene="fadA"
                     /locus_tag="ACHU0J_001254"
     CDS             complement(24252..25415)
                     /gene="fadA"
                     /locus_tag="ACHU0J_001254"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1216014.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25425..27614)
                     /gene="fadB"
                     /locus_tag="ACHU0J_001255"
     CDS             complement(25425..27614)
                     /gene="fadB"
                     /locus_tag="ACHU0J_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1216015.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27804..29135
                     /gene="pepQ"
                     /locus_tag="ACHU0J_001256"
     CDS             27804..29135
                     /gene="pepQ"
                     /locus_tag="ACHU0J_001256"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1216016.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29135..29749
                     /locus_tag="ACHU0J_001257"
     CDS             29135..29749
                     /locus_tag="ACHU0J_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1216017.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29788..31239
                     /gene="trkH"
                     /locus_tag="ACHU0J_001258"
     CDS             29788..31239
                     /gene="trkH"
                     /locus_tag="ACHU0J_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1216018.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31251..31796
                     /gene="hemG"
                     /locus_tag="ACHU0J_001259"
     CDS             31251..31796
                     /gene="hemG"
                     /locus_tag="ACHU0J_001259"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1216019.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7440 a         8143 c         8746 g         7676 t
ORIGIN      
        1 agatgttggg ataaacatac gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag
       61 catggcaata tgcccactct catacaagag tcggcatatt ttttcagaat atatttattt
      121 tttatttgga cgttctacgc tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca
      181 cccaaataaa ggtgaaagtc accatcttat cggcacccgg tttttcacca taaaacgtca
      241 cagccagcag gaacatcagc gtcgggccaa tgtactgaaa aaagcctaac gttgagagac
      301 gcaggcgcgt ggcagcggca gtaaaacaca acagcggtac ggtagtgaca ataccggcgg
      361 cgatcagcag taaattcagc gacatcgggt tttgccccat atggctggtt gaactgtcgg
      421 caatagcaaa caggtaaatc gccgccacgg gcagcagcca catggtttca attaacatgc
      481 cggtttgtgc ttctaccgca atcttcttgc gcaccaggcc gtagaaggca aaactaaatg
      541 ccagccccag cgcgataata ggtagcgaac caaaagtcca cagctggacc aacacgccac
      601 atatcgccag aatcaccgcc agccattgca tccggcggaa tcgctcgccg aggaaaatca
      661 tccccagcac aatgttcacc agcgggttaa taaagtaacc aaggctcgct tccagcatat
      721 ggtgattgtt caccgcccag ataaacagta gccagttgcc accaatcagt acggcagaga
      781 ctgccagcat aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag gaccactggc
      841 ggcaaatgct catcagcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga
      901 tctcatcggc gggcacgtag taaatcagct tgaagtatgc tggcgcaata ccccaaataa
      961 aataagcggc aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata
     1021 ctcattttta attggtaaca gcagtttacc tgcttttatg tcttcaacct accatatagg
     1081 tggcggtggc actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg
     1141 cgactttatt gcctgcacgc aacaggctac tggtagcagt aaaacgctcg cccctgcctg
     1201 ggcgcagata atcaacgcga agatcaatgg tccccatccg cgatagccgc tggcgtagtt
     1261 catcttcact gatggtttcg tggcgggtta aggtacttcc cacgcacacc agaccggcgg
     1321 cgacatccag cgccgacgca atgactccgc cgtgcaaaat gctttgcgcc cagttaccca
     1381 ccatcatcgg ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagtt
     1441 ccatccccaa tgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca
     1501 gggcttgttc agcggtcagt acggcagaca tatcatcctt acacttcatt ggttaatgaa
     1561 atgttgattt tatgcttctt tgttgttggt ttctacttta ggaagggata actaacggca
     1621 atggagttaa gtatgtaaaa tagcccgcag aaaaacattc accttatcaa taattcgtta
     1681 cggagaacac gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct
     1741 atggcagtat atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt
     1801 ggcagtatta tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat
     1861 gacaccaact acctcatcta cacccaaacc agcgatctga ataaagaagc gattgccagt
     1921 tacgactggg cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt
     1981 ccgctgtggc gtgggatttt aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa
     2041 tcctggtggc aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa
     2101 ccgcaattgt tcctcggttt tgccaccgat taccgttttg caggttggac gctgcgcgat
     2161 gtggagatgg ggtataacca cgactctaac gggcgttcag acccgacctc ccgcagctgg
     2221 aaccgccttt atactcgcct gatggcagaa aacggtaact ggctggtaga agtgaagccg
     2281 tggtatgtgg tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac
     2341 cagcttaaaa tcggctatca cctcggtgat gcggtgctca gtgcgaaagg acagtacaac
     2401 tggaacaccg gctacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg
     2461 cgcctttata ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac
     2521 cagacccgtg tcggtgtagg ggttatgcta aacgacttgt tttgatgaac ggttgagtgg
     2581 ttggcaaatc tggaatccag catccaggat taccctctca gagactaaaa gcattgcagt
     2641 ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg
     2701 gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac aggttttaca agaaaccttt
     2761 ggctaccaac agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc
     2821 gattgcctgg ttgttatgcc cactggtggc ggaaaatccc tttgctatca aattcctgcc
     2881 ttattgctaa acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag
     2941 gtggatcaac tacaggccaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc
     3001 gaacagcaac ttgaagtgat gacaggctgc cgcaccgggc aaattcgctt actgtatatc
     3061 gccccggaac gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg
     3121 ttattagccg tcgatgaagc gcactgtatc tcccaatggg gtcacgattt ccgcccggaa
     3181 tatgccgcgc tcggtcagtt gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc
     3241 gccacagccg acgacaccac gcgccaggat atcgtgcgcc tgctggggct gaacgatccg
     3301 ctgattcaaa tcagcagttt tgaccgtccg aatattcgct acatgctgat ggagaaattc
     3361 aaaccgctcg atcagttgat gcgctacgtg caggaacagc gcggtaagtc tggcattatc
     3421 tactgcaaca gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caggggtatt
     3481 agcgcggcgg cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa
     3541 ttccagcgcg atgatctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat
     3601 aaaccaaacg ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat
     3661 caggaaaccg gtcgcgccgg gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat
     3721 ccggctgata tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag
     3781 gatatcgagc gccacaaact caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt
     3841 cgtctggtat tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat
     3901 atctgcctcg atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc
     3961 accattggtc gtgtgaatca gcggtttggg atgggttatg tggtggaagt gattcgtggt
     4021 gctaataacc agcgtatccg cgactatggt catgacaaac tgaaagtcta tggtatgggc
     4081 cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc agctgattca tctcggcctg
     4141 gtgacgcaaa atattgccca gcattctgcc ctacaactga cagaggccgc gcgcccggtg
     4201 ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa
     4261 gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa attacgcaaa
     4321 ctgcgtaaat cgattgccga tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca
     4381 accttgattg agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac
     4441 ggcgttggga tgcgcaagct ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg
     4501 catgtcgatg gcgacgacga agagtagtca gcagcataaa aaagtgccag tatgaagact
     4561 ccgtaaacgt ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg
     4621 gtgcacattg tggcgcttat gagccccggt cccgatttct tttttgtctc tcagaccgct
     4681 gtcagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg
     4741 gtttgggctg ggattgcgtt gcttggcctg catttgatta tcgaaaaaat ggcctggctg
     4801 catacgctga ttatggtagg cggtggcctg tatctctgct ggatgggtta ccagatgcta
     4861 cgcggtgcac tgaaaaaaga ggctgtatct gcgcctgcac cacaggttga gctggcgaaa
     4921 agcgggcgca gtttcctgaa aggcttactg accaatctcg ccaatccgaa agcgattatc
     4981 tattttggtt cggtattctc attgtttgtc ggcgataacg ttggcactac cgagcgctgg
     5041 ggcattttcg cgttgattat tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg
     5101 tttgccctgc cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt
     5161 gccggggcgt tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg
     5221 cgtcttcaga gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat
     5281 gcctcgccga tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca
     5341 gcgccttcat ctgctttggt cctttaatcc atagagcaat cctttgagca agggtggcgt
     5401 aaccgatcat cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca
     5461 tcagttgcgg ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac
     5521 ttttgggatt ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag
     5581 tagaggccag cgatttaaga tcaattgcgc cagcggcgcg ccactgctgg attcccagcc
     5641 aaatcaagta agccgcgcct gcccacttta acacttcaaa cgcaatcact gagcgggaaa
     5701 atagcgtccc caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc
     5761 cagcaataga cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga
     5821 ttgcaccaga gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc
     5881 attctaaggt catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc
     5941 gcagcgttgt gtcaggcacg cttaaaaaaa cgattttacg tggtttaaga ggcagattac
     6001 ccgatgtttc agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc
     6061 atgggaccgc tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat
     6121 gacattccgg tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc
     6181 tgcccggggc gtattgagag ctacgtaaaa tatgcggaac tggcctacga cctgttccat
     6241 ttggggtttg atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta
     6301 gccgatccgc atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca
     6361 ttctggcagc aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg
     6421 atgggcggtg cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt
     6481 gcgctaactg cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag
     6541 atcctcaact gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg
     6601 cgctggcgcg cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga
     6661 cgtaacttac gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat
     6721 tgggtacgcg aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc
     6781 acgcaaacgc ttctcttaca agctgaagag gaacgcgtgg tggataaccg catgcacgac
     6841 cgtttttgtg aactccgcac cgccgcgggc catcctgtcg aaggaggacg gccgttggta
     6901 ataaaaggtg cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc
     6961 cacgccatcg ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag
     7021 gtttaaattt cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc
     7081 cgaccatacg ttatcccctt acgccaaaga aactctgaag ctgctcaccg cgcgcggcat
     7141 caactttgtg tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct
     7201 ggagattaag tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa
     7261 tctgattttt gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa
     7321 cgacaatccg gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca
     7381 tcgcccggaa gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc
     7441 gggattactg gagccggaag gcatcagcaa agtgtttttc acctgcgatt cccatgaaca
     7501 actgctgccg ctggagcagg cgattaacgc tcgctggggc gatcgcgtca acgtcagttt
     7561 ctctacctta acctgtctgg aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga
     7621 agcagtggcg aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat
     7681 gaacgacgcc gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca
     7741 gcgtctgaaa gatctgcatc cggagctgga agttattggt actaatgccg acgacgcggt
     7801 gccgcattat ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa
     7861 cctgatactt cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgttact
     7921 tatcatcacc acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg
     7981 gcacgtcgat agctattttg cggtgctggt gcgcgttggc ttggcggcac tcgtttttct
     8041 gccgtttctg cgtacccgta gcaatagcct gaaaacggtc ggcctgtata tgctggtggg
     8101 cgcgatgcag ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt
     8161 ctccgaattg ttgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat
     8221 catgagtaag cgccgtctgc gctggggcta tgcttttagc gccttgctgg cggtgattgg
     8281 tgccgggatt attcgctatg atcaggtcac cgaccatttc tggactggct tgttgctggt
     8341 gcaactctcc aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac
     8401 tcgcccgatg ccgcagtata acgcctttgc gtggttctat cttggcgcgt tcctggtggc
     8461 ggtaattgca tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg
     8521 gggcattctg gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta
     8581 cggcgcgacg caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc
     8641 agggctgctg gtaaacctgg ctatctggca ccaacagccg cactggccaa cgtttattac
     8701 aggcgcgctg gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc
     8761 ttcacaaacg gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta
     8821 actggctgac gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg
     8881 cccagggttt tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt
     8941 agcgcggcaa tacccatccg cgcggcaacc atttgaatca acaataaggt gttatcgacg
     9001 cttttcagtg acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga
     9061 ctacgctgca ccggataaat taatagcgtc tcgctggcga gatcttccgg tgtaatacgc
     9121 gtttttgccg ccagtggatg gtcaggtgct aacaccaggc gtacttcata atcgaacatc
     9181 ggcgaataat gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg
     9241 ccctgttgca acgcgggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc
     9301 tgcggccagt tcttatggaa atttcctaaa gcgggtgtca gccactgaat acagctatgg
     9361 cactcaatgg caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg
     9421 ctgatttgcg gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag
     9481 cgtagcggct ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta
     9541 aactggtgag acagggcgga ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag
     9601 ccgcagttcc gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt
     9661 cacttcggca tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg
     9721 taaacatctg gacggctaaa atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt
     9781 ctccaccccg gtcacttact tcagtaagct cccggcgatg aataaacttg ccgccttccc
     9841 taaattcaaa atccatagga tttacatata attagaggaa gaaaaatgac aatattgaat
     9901 cacaccctcg gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt
     9961 tattgggcgg ggaactccac gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct
    10021 cgtcactggg atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc
    10081 tggtacgatc atgtactgac caccagtttg ctgctgggta atgttccgcc acgtcatcag
    10141 aacaaagacg gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg
    10201 actggcgaac ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac
    10261 atggtgccgg agttcgttaa aggccaacag ttcaaactga cttggacgca gctgctggag
    10321 gaagtggacg aggcgctggc gctgagccac aacgttaagc ctgtgctgct ggggccggtt
    10381 acttacctgt ggctggggaa agtgaaaggt gaacagtttg accgccttag cctgctgaac
    10441 gacattctgc cggtttatca gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg
    10501 gtacagattg atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac
    10561 aaacccgctt acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa
    10621 ggcgtaacgc caaatctcga cacgattact gcgctgcctg ttcaggggct gcatgtggac
    10681 cttgtacatg gtaaagataa cgttgttgaa ctgcacaagc gcctgccttc tgactggctg
    10741 ctgtctgcgg gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat
    10801 gcgcaaatta aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg
    10861 ctgcacagcc ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg
    10921 tttgccttcg ccctacaaaa atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt
    10981 ggtgacacgg cagctctggc agagtggagc gccccaattc aggcgcgtcg tcactctact
    11041 cgtgtacata atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag
    11101 cgtgcgaatg tctatgaagt gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg
    11161 tggccgacca ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg
    11221 gatttcaaaa agggcaatct cgacgccaac aactaccgca cgggcattgc ggaacatatc
    11281 aagcaggcca ttgttgagca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc
    11341 gagcgtaatg acatggtgga atactttggc gagcacctcg acggatttgt ctttacgcaa
    11401 aacggttggg tacagagcta cggttcccgc tgcgtgaagc caccgattgt cattggtgac
    11461 attagccgcc cggcaccgat taccgtggag tgggcgaagt atgcgcaatc gctgaccgac
    11521 aaaccggtga aagggatgct gacggggccg gtgaccatac tctgctggtc gttcccgcgt
    11581 gaagatgtca gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg
    11641 gccgatctgg aagccgctgg aattggcatc atccagattg acgaaccggc gctgcgcgaa
    11701 ggtttaccgc tgcgtcgtag cgactgggat gcgtatctcc agtggggcgt agaggccttc
    11761 cgtatcaacg ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc
    11821 gagttcaacg acatcatgga ttcgattgcg gcgctggacg cagacgtcat caccatcgaa
    11881 acctcgcgtt ccgacatgga gttgctggag tcgtttgaag agtttgatta tccaaatgaa
    11941 atcggtcctg gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa
    12001 gccttgctga agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt caacccggac
    12061 tgtggcctga aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag
    12121 gcggcgcaga atttgcgtcg ggggtaaatc caaaccgggt ggtaatacca cccggtcttt
    12181 tctcattaca gcgacttctt cccaccatac tgcttaaacc attccagcat acgctgccag
    12241 ccatctttcg cagattcggc atgatagctc gggcgataat cagcgttgaa tgcatgcccg
    12301 gcatccgggt acacgataat ctctgctttc gcattagcag cccgcagcgc ctggcgcatc
    12361 gtttcaacgc tctcctgcgg aatgctgtta tcctgaccac catataagcc gagaaccggc
    12421 gcgttaagat cggttgcgat atcaacaggt tgtttcggtg aattcagcga cttgtcgccc
    12481 gtcagtttgc cgtaccacgc cactgcggct tttagctgtg gattatgcgc ggcatacagc
    12541 caggtgatac gtccacccca gcagaatccg gtgatcatta aacgatgaac atcgccgccg
    12601 ttgcgggaag cccaactggc gacatgatcg agatcggcca gcacctgcga gtcaggcact
    12661 tttgctacca gaccgctaag caacgtgggg atatcggcaa aatcattcgg atcgccttcg
    12721 cggaagtaaa gttcaggtgc gatagccaga tacccctcca gcgccagacg gcgacaaatg
    12781 tcgcggatat gttcatgcac gccaaaaatt tcctgcacta caatgaccac tggcagtggg
    12841 ccatcgcttt gctttggtct ggcatggtaa gcaggcatgt tatccccttg tgaagggata
    12901 gaggtgaagc ccgccacaat cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc
    12961 gatgcagcag gtgcaaatcc agattgttgt gttgttgcca tggtattctc cgtaccctta
    13021 taaaaatgtt gcgcaatgtt aactatagtc agcatgcaac aaatcacatt gcctgaatcg
    13081 gctcatcttt tatgcagtcc tgcagaatga agagtgattt atgtgatatg aatcactttt
    13141 ggtgggtaaa tttatgcaac gcatttgcgt catggtgatg agtatcacga aaaaatgtta
    13201 aacccttcgg taaagtgtct ttttgcttct tctgactaaa ccgattcaca gaggagttgt
    13261 atatgtccaa gtctgatgtt tttcatctcg gcctcactaa aaacgattta caaggggcta
    13321 cgcttgccat cgtccctggc gacccggatc gtgtggaaaa gatcgccgcg ctgatggata
    13381 agccggttaa gctggcatct caccgcgaat tcactacctg gcgtgcagag ctggatggta
    13441 aacctgttat cgtctgctct accggtatcg gcggcccgtc tacctctatt gctgttgaag
    13501 agctggcaca gctgggcatt cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc
    13561 cgcatattaa tgtgggtgat gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga
    13621 gcctgcactt cgcaccgctg gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc
    13681 tggttgaagc tgcgaaatct attggcgcga caactcacgt tggcgtgaca gtttcttctg
    13741 ataccttcta cccaggtcag gaacgttacg atacttactc tggtcgcgta gttcgtcact
    13801 ttaaaggttc tatggaagag tggcaggcga tgggcgtaat gaactatgaa atggaatctg
    13861 caactctgct gaccatgtgt gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta
    13921 tcgttaaccg cacccagcaa gagatcccga atgctgagac gatgaaacaa accgaaagcc
    13981 atgcggtgaa aatcgtggtg gaagcggcgc gtcgtctgct gtaattctct tctcctgtct
    14041 gaaggccgac gcgttcggcc ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc
    14101 caactggacg tttgtacagc acaattctat tttgtgcggg taagttgttg cgtcaggagg
    14161 cgttgtggat ttctcaatca tggtttacgc agttattgcg ttggtgggtg tggcaattgg
    14221 ctggctgttt gccagttatc aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga
    14281 agagatggtc gcggagttaa gcgcggcaaa acaacaaatt acccaaagcg agcactggcg
    14341 tgcagagtgt gaattgctca ataacgaagt gcgcagcctg caaagtatta acacctctct
    14401 ggaggccgat ctgcgtgaag taaccacgcg gatggaagcc gcacagcaac atgctgacga
    14461 taaaattcgc cagatgatta acagcgagca gcgcctcagt gagcagtttg aaaacctcgc
    14521 caaccgtatt tttgagcaca gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa
    14581 cagcctgttg tcgccgctac gtgaacaact ggacggtttc cgccgtcagg ttcaggacag
    14641 cttcggtaaa gaagcacaag aacgccatac cctgacccac gaaattcgca atctccagca
    14701 actcaacgcg caaatggccc aggaagcgat caacctgacg cgcgcgctga aaggcgacaa
    14761 taaaacccag ggcaactggg gcgaggtagt attgacgcgg gtgctggagg cttccggtct
    14821 gcgtgaaggg tatgaatatg aaacccaggt cagcatcgaa aatgacgccc gctcgcggat
    14881 gcagccggat gtcatcgtgc ggctgccgca gggaaaagat gtggtgatcg acgccaaaat
    14941 gacgctggtc gcctatgaac gctattttaa cgccgaagac gactacaccc gcgaaagcgc
    15001 gctacaggaa catatcgcgt cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta
    15061 tcaacagctg ccggggctgc gaactctgga ttacgtgctg atgtttattc ccgttgaacc
    15121 cgctttttta ctggcgcttg accgccagcc ggagctgatc accgaagcgt tgaaaaacaa
    15181 tatcatgctg gttagcccga ctacgctgct ggtggcgttg cgtactattg ccaacctgtg
    15241 gcgttatgag catcaaagcc gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta
    15301 cgacaagatg cgtttgttca tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc
    15361 gcaggataac taccgccagg caatgaaaaa actctcttca ggtcgcggaa atgtgctggc
    15421 gcaggcagaa gcgtttcgcg gtttaggagt agaaattaaa cgcgagatta atccggattt
    15481 ggctgaacag gcggtgagcc aggatgaaga gtatcgactt cggtcggttc cggagcagcc
    15541 gaatgatgaa gcttatcaac gcgatgatga atataatcag cagtcgcgct agcccattag
    15601 gggtagttaa gccgggtaga aatctagggc atcgacgccc aatctgttac acttctggaa
    15661 caattttttg atgagcaggc attgagatgg tggataagtc acaagaaacg acgcactttg
    15721 gttttcagac cgtcgcgaag gaacaaaaag cggatatggt cgcccacgtt ttccattccg
    15781 tggcatcaaa atacgatgtc atgaatgatt tgatgtcatt tggtattcat cgtttgtgga
    15841 agcgattcac gattgattgc agcggcgtac gccgtgggca gaccgtgctg gatctggctg
    15901 gtggcaccgg cgacctgaca gcgaaattct cccgcctggt cggagaaact ggcaaagtgg
    15961 tccttgctga tatcaatgaa tccatgctca aaatgggacg cgagaagctg cgtaatatcg
    16021 gtgtgattgg caacgttgag tatgttcagg cgaacgctga ggcgctgccg ttcccggata
    16081 acacctttga ttgcatcacc atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag
    16141 cactgcgttc aatgtatcgc gtgctgaaac ccggcggccg cctgctggtg cttgagttct
    16201 cgaagccaat tatcgagccg ctgagcaaag cctatgatgc atactccttc catgtgctgc
    16261 cgcgtattgg ctcactggtc gcgaacgacg ccgacagcta ccgttatctg gcagaatcca
    16321 tccgtatgca tcccgatcag gataccctga aagccatgat gcaggatgcc ggattcgaaa
    16381 gtgtcgacta ctacaatctg acggcagggg ttgtggcgct gcatcgtggt tataagttct
    16441 gacaggagac cggaaatgcc ttttaaacct ttagtgacgg caggaattga aagtctgctc
    16501 aacaccttcc tgtatcgctc acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa
    16561 gtattgcgcg tggaggtaaa aggcttttcg acgtcattga ttctggtgtt cagcgaacgc
    16621 caggttgatg tactgggcga atgggcaggc gatgctgact gcaccgttat cgcctacgcc
    16681 agtgtgttgc cgaaacttcg cgatcgccag cagcttaccg cactgattcg cagtggtgag
    16741 ctggaagtgc agggcgatat tcaggtggtg caaaacttcg ttgcgctggc agatctggca
    16801 gagttcgacc ctgcggaact gctggcccct tataccggtg atatcgccgc tgaaggaatc
    16861 agcaaagcca tgcgcggagg cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt
    16921 tatgtggcgg aagccattac tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc
    16981 tggtttgcgg aagagacggc tgccgtcgag cgtgctgttg atgccctgac caaacggctg
    17041 gaaaaactgg aggctaaatg acgccaggtg aagtacggcg cctatatttc atcattcgca
    17101 cttttttaag ctacggactt gatgaactga tccccaaaat gcgtatcacc ctgccgctac
    17161 ggctatggcg atactcatta ttctggatgc caaatcggca taaagacaaa cttttaggtg
    17221 agcgactacg actggccctg caagaactgg ggccggtttg gatcaagttc gggcaaatgt
    17281 tatcaacccg ccgcgatctt tttccaccgc atattgccga tcagctggcg ttattgcagg
    17341 acaaagttgc tccgtttgat ggcaagctgg cgaagcagca gattgaagct gcaatgggcg
    17401 gcttgccggt agaagcgtgg tttgacgatt ttgaaatcaa gccgctggct tctgcttcta
    17461 tcgcccaggt tcataccgcg cgattgaaat cgaatggtaa agaggtggtg attaaagtca
    17521 tccgcccgga tattttgccg gtcattaaag cggatctgaa acttatctac cgtctggctc
    17581 gctgggtgcc gcgtttgctg ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg
    17641 agtacgaaaa gaccttgatt gatgaactga atttgctgcg ggaatctgcc aacgccattc
    17701 agcttcggcg caattttgaa gacagcccga tgctctacat cccggaagtt taccctgact
    17761 attgtagtga agggatgatg gtgatggagc gcatttacgg cattccggtg tctgatgttg
    17821 cggcgctgga gaaaaacggc actaacatga aattgctggc ggaacgcggc gtgcaggtgt
    17881 tcttcactca ggtctttcgc gacagctttt tccatgccga tatgcaccct ggcaacatct
    17941 tcgtaagcta tgaacacccg gaaaacccga aatatatcgg cattgattgc gggattgttg
    18001 gctcgctaaa caaagaagat aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc
    18061 gcgactatcg caaagtggca gagctacacg tcgattctgg ctgggtgcca ccagatacca
    18121 acgttgaaga gttcgaattt gccattcgta cggtctgtga acctatcttt gagaaaccgc
    18181 tggccgaaat ttcgtttgga catgtactgt taaatctgtt taatacggcg cgtcgcttca
    18241 atatggaagt gcagccgcaa ctggtgttac tccagaaaac cctgctctat gtcgaagggg
    18301 taggacgcca gctttatccg cagctcgatt tatggaaaac agcgaagcct ttcctggagt
    18361 cgtggattaa agatcaggtc ggtattcctg ctctggtgag agcatttaaa gaaaaagcgc
    18421 cgttctgggt cgaaaaaatg ccagaactgc ctgaactggt ttacgacagt ttgcgccagg
    18481 gcaagtattt acagcacagt gttgataaga ttgcccgcga gcttcagtca aatcatgtac
    18541 gtcagggaca atcgcgttat tttctcggaa ttggcgctac gttagtatta agtggcacat
    18601 tcttgttggt cagccgacct gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc
    18661 tgatcgcctg gtttgtcggt tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg
    18721 ccgtgtaacg tataatgcgg ctttgtttaa tcatcatcta ccacagagga acatgtatgg
    18781 gtggtatcag tatttggcag ttattgatta ttgccgtcat cgttgtactg ctttttggca
    18841 ccaaaaagct cggctccatc ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag
    18901 caatgagcga tgatgaacca aagcaggata aaaccagtca ggatgctgat tttactgcga
    18961 aaactatcgc cgataagcag gcggatacga atcaggaaca ggctaaaaca gaagacgcga
    19021 agcgccacga taaagagcag gtgtaatccg tgtttgatat cggttttagc gaactgctat
    19081 tggtgttcat catcggcctc gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa
    19141 cggtagcggg ctggattcgc gcgttgcgtt cactggcgac aacggtgcag aacgaactga
    19201 cccaggagtt aaaactccag gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc
    19261 tcactaacct gacgcccgaa ctgaaagcgt cgatggatga actacgccag gccgcggagt
    19321 cgatgaagcg ttcctacgtt gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca
    19381 tccataaccc ggtggtgaaa gataatgaag ctgcgcatga gggcgtaacg cctgccgctg
    19441 cacaaacgca ggccagttcg ccggaacaga agccagaaac cacgccagag ccggtggtaa
    19501 aacctgctgc ggacgctgaa ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac
    19561 cgtaaacatg tctgtagaag atactcaacc gcttatcacg catctgattg agctgcgtaa
    19621 gcgtctgctg aactgcatta tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc
    19681 caatgacatc tatcacttgg tatccgcgcc attgatcaag cagttgccgc aaggttcaac
    19741 gatgatcgcc accgacgtgg cctcgccgtt ctttacgccg atcaagctga cctttatggt
    19801 gtcgctgatt ctgtcagcgc cggtgattct ctatcaggtg tgggcattta tcgccccagc
    19861 gctgtataag catgaacgtc gcctggtggt gccgctgctg gtttccagct ctctgctgtt
    19921 ttatatcggc atggcattcg cctactttgt ggtctttccg ctggcatttg gcttccttgc
    19981 caataccgcg ccggaagggg tgcaggtatc caccgacatc gccagctatt taagcttcgt
    20041 tatggcgctg tttatggcgt ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct
    20101 gtgctggatg gggattacct cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt
    20161 tggtgcattc gttgtcggga tgttgctgac gccgccggat gtcttctcgc aaacgctatt
    20221 ggcgatcccg atgtactgtc tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg
    20281 taaagggcga aatcgggaag aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga
    20341 ataaattcaa ccgcccgtca gggcggttgt catatggagt acaggatgtt tgatatcggc
    20401 gttaatttga ccagttcgca atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt
    20461 gacgcgggag ttaatgggct actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg
    20521 caaaagctgg cgcgtcagta ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac
    20581 agcagccagt ggcaagctgc gactgaagaa gcgattattg agctggccgc gcagccagaa
    20641 gtggtggcga ttggtgaatg tggtctcgac tttaaccgta acttttcgac gccggaagag
    20701 caggaacgcg cttttgttgc tcagctacgc attgccgcag aattaaacat gccggtattt
    20761 atgcactgtc gcgatgccca cgagcggttt atgacattgc tggagccgtg gctggataaa
    20821 ctgcctggtg cggttcttca ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg
    20881 gcgcgtggaa tttatatcgg cattaccggt tgggtttgcg atgaacgacg cgggctggag
    20941 ctgcgggaat tgttgccgtt gattccggcg gagaaattgc tgatcgaaac agatgcgccg
    21001 tatctgctcc cgcgcgatct cacgccaaag ccatcatccc ggcgcaacga gccagcccat
    21061 ctgccccata ttttgcaacg tattgcgcac tggcgtggag aagatgccgc atggctggct
    21121 gccaccacgg atgccaatgt caaaacactg tttgggattg cgttttagag tttgcggaac
    21181 tcggtgttct tcacactgtg cttaatctct ttattaataa gattaagcaa tagcatggag
    21241 cgagcctcac catcgggttc ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta
    21301 ataatcacct tatcacccgg ataagggggg gccggatcga caatgtcttt cggtttatat
    21361 accgatagct gatgaattac cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca
    21421 aagtggctga cgccacgcgt agcgctgatg gtcgtggtat gaatcacttc tggatcaaat
    21481 tccacaaaca ggtagttagg gaataacggt tcactgactg cggtacgttt accccgtacg
    21541 attttttcca gggtgatcat cggagcgagg caattaaccg cctgtctctc aaggtgttcc
    21601 tgggcacgtt gaagttgccc gcgcttgcag tacagtaaat accaggattg cataatgact
    21661 cttatccgtt taatcggggc gcaaggatag caaaagcttt acgctaagtt aattatattc
    21721 cccggtttgc gttataccgt cagagttcac gctaatttaa caaatttaca gcattgcaaa
    21781 gatgaacgcc gtataatggg cgcagattaa gaggctacaa tggacgccat gaaatataac
    21841 gatttacgcg acttcctgac gctgcttgaa cagcagggtg agctaaaacg tatcacgctc
    21901 ccggtggatc cgcatctgga aatcactgaa attgctgacc gcactctgcg tgctggtggg
    21961 cctgcgctgt tgttcgaaaa ccctaaaggc tactcaatgc cggtgctgtg caacctgttc
    22021 ggtacgccaa agcgcgtggc gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa
    22081 gttggtaaat tattggcgtt tctgaaagag ccggagccgc caaaaggttt ccgcgacctg
    22141 tttgataaac tgccgcagtt taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt
    22201 gcgccctgcc aacaaaaaat cgtctctggc gatgacgtcg atctcaatcg cattcccatt
    22261 atgacctgct ggccggaaga tgccgcgccg ctgattacct gggggctgac cgtaacgcgc
    22321 ggcccgcata aagagcggca gaatctgggc atttatcgcc agcagctgat tggtaaaaac
    22381 aaactgatta tgcgctggct gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt
    22441 gcggcgcatc cgggcgaacg tttcccggtt tctgtggcgc tgggtgccga tcctgccacg
    22501 attctcggtg cagtcacccc cgttccggat acgctttcag agtatgcgtt tgccggattg
    22561 ctacgcggca ccaaaaccga agtagtaaag tgtatttcca atgacctcga agtgccagcc
    22621 agtgccgaaa tcgtcctgga agggtatatc gatcctggtg agatggcgcc ggaagggccg
    22681 tatggtgacc acacaggtta ctataatgaa gtcgataatt tcccggtgtt taccgtgacg
    22741 catattaccc agcgtgaaga tgcgatttac cattccacct ataccgggcg tccgccagat
    22801 gagcccgcgg tactgggcgt ggcgttgaac gaagtgtttg taccgattct gcaaaaacag
    22861 ttcccggaaa ttgtcgattt ttacctgccg ccggaaggct gttcttatcg tctggcggta
    22921 gtgacgatca aaaaacagta cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg
    22981 ttcttacgcc agtttatgta cactaaattt gtgatcgttt gcgatgatga cgtcaacgca
    23041 cgcgactgga acgatgtgat ttgggcgatt accacccgta tggacccggc gcgggatact
    23101 gttctggtag aaaatacgcc tattgattat ctggattttg cctcgcctgt ctctgggctg
    23161 ggttcaaaaa tggggctgga tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg
    23221 ggacgtccca tcaaaaaaga tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa
    23281 ctggctattt ttaacaacgg taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg
    23341 atccgacaga gaaagcgcat gacaacctta agctgtaaag tgacctcggt agcagctatc
    23401 acggataccg tatatcgtgt ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt
    23461 cagtatttga tggtagtgat ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg
    23521 ccggatgaaa aagggtttat cgagctgcat attggcgctt ctgaaatcaa cctttacgcg
    23581 aaagcagtca tggaccgcat cctcaaagat catcaaatcg tggtcgacct tccccacgga
    23641 gaagcgtggc tgcgcgatga tgaagagcgt ccgatgattt tgattgcggg cggcaccggg
    23701 ttctcttatg cccgctcgat tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc
    23761 accatttact ggggcgggcg tgaagagcag catctgtatg atctctgcga gcttgaggcg
    23821 ctttcgttaa agcaccttgg tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc
    23881 tggcgtgggc gtactggtac cgtgttaacg gcggtattgc aggatcatgg tacgctggca
    23941 gagcatgata tctatattgc cggacgtttt gagatggcga aaattgcccg tgacctgttt
    24001 tgcagtgagc gtaatgcgcg ggaagatcgc ctgtttggcg atgcgtttgc atttatctga
    24061 gatataaaaa aacccgcccc tgacaggcgg gaagaacggc aactaaaact ttattcagtg
    24121 gcatttagat ctatgtcgca tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc
    24181 gtcttatcag gcctacattg gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat
    24241 ccggcaagtg gttaaacccg ctcaaacacc gtcgcaatac cctgacccag accgatacac
    24301 atcgtcgcca gaccaaactg aacgtctttg cgttccatca gattcagcag cgtggtgctg
    24361 atacgcgcac cggaacaacc cagcggatga cccagcgcga tcgcaccacc attgaggttg
    24421 atcttctcgt caatctgctc cattagcccg agatctttaa tgcacggcag gatctgcgcg
    24481 gcaaaggctt cgttcatctc aaacacgccg atatcgctgg cagaaagccc cgcttttttc
    24541 agcgccagct tcgaggccgg aaccgggccg taacccataa tcgatgggtc acaaccaacg
    24601 accgccatcg aacgcacacg agcgcgcggc ttaagaccta attcatgggc gcggctttca
    24661 ctcatcacca gcatggcagc tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc
    24721 gtaccgttta ctggatcaaa cgccggacgc agcgtggcga gggcttccac ggtggtttcc
    24781 gggcgaatca cttcgtcgta attaaactgc ttcaggacgc cgtcggcatc gtgaccacca
    24841 gttgggatga tttcattttt aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt
    24901 gaccgcgcgg caaaggcatc ctgcatttca cggctgatac cgtgcatacg cgccagcatt
    24961 tctgccgtta agcccatcat gcccgccgct ttggcgacat tgcggctcag gccggggtga
    25021 aaatcgacgc cgtgactcat cggcacatgg cctatatgct ccacgccgcc aaccagacat
    25081 gcctgcgtat cgccagtcat gatcattcgt gctgcgtcat gcagtgcctg catggatgaa
    25141 ccacacaagc gattgacggt aaccgccggg acagagtgcg gcacttctgc cagcagcgcc
    25201 gcgttacggg cgatgttaaa accctgctcc agcgtctgtt gcacacaacc ccagtaaata
    25261 tcgtcgaggg ccgccgcttc cagcgccggg ttacgcgcca gcaggctacg cattaaatga
    25321 gcggagagat cttccgcacg cacgttacga aaagcaccgc ccttcgaacg gcccatcggg
    25381 gtgcgaattg catcgacaat gacaacctgt tccattgtga ctccttaagc cgttttcagg
    25441 tcgccaaccg gacgggctgg ctcaaccgga ggatagtacg gttcgttatg acgcgcttta
    25501 ttacgcagac cttccggcac ttcatacagc gggccgaggt gctgatattg ctgtgccata
    25561 tcgaggtatt ttgcgctacc gagggtgtcc agccagcgga acgcgccgcc gtggaacgga
    25621 gggaagccca ggccgtagac cagcgccata tccgcttccg ccggagtggc gataatgccc
    25681 tcttccagac agcgcaccac ttcgttgacc atcgggatca tcatgcgggc gataatctct
    25741 tcttcgctga aatcgcgctt cggttggctc acttctgcca gcaggtcttc aacggcggcg
    25801 tcttcttctt tcttcggctt acctttgctg tcttctttat aacgccagaa accgaggccg
    25861 ttcttctgac caaagcggtt ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc
    25921 tgcatccgct gcgggaagcc tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg
    25981 cccacaacgt ccagcagata tgccgggccc atcggccagc caaactgttt ttccatcact
    26041 ttgtcgatct tgcggaaatc cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac
    26101 gggaacagca cgcggttaac aaagaagccg gggcagtcgt taaccacaat cggcgtcttg
    26161 cccatcttgc tcgcccaggc gacaactttc gcgatggttt cgtcggagct tttctcgccg
    26221 cgaataattt ctaccaacgg cattcggtgg accgggttaa agaagtgcat cccgcagaag
    26281 ttttccgggc gttccagcgc gttggccagt tcgctgatag gaatggttga agtgttagac
    26341 gccagcacgg tatcctggcg tactttttgt tcggtttctg ccagtacggc ttttttcact
    26401 ttcgggtttt caacaaccgc ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg
    26461 agcgttgggt ggatcgtgga gatcacgcca gccagtttca gaccatcgat tttgccgcgc
    26521 tcaagctgct tgttcagcag tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg
    26581 ttgatatctt tcatgacaac cggcacgcct ttccacgcgg actggtaagc gataccgcca
    26641 cccatgatgc ctgcgcccag cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt
    26701 ttcttcgctt tgccttttac atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct
    26761 tcgttggtat gcgccagcgg gacaaaactt ttgttttcca gatttaaggc ttcttcacga
    26821 ccaaaacggg ccgcagcttc aatggttttt actgcggtga tgggggccgg ataatgtttc
    26881 cccgccgttt gcgcgaccat tcctttagcg atggtgaagc tcatggtggc ttcaatcttg
    26941 ctcagtttta gcggttcctg cttcggctga cgtttcgctt tccagtcgag gtcgccgtta
    27001 atggcctggc gtaaaatcgc catcgcccct tcaatcagtt tttctgcttt gactacgcca
    27061 tccaccagac cgattttcag cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt
    27121 tccagtgcac tgtcagcgcc cagcatacgt ggcatacgta cagaaccgcc aaagcccggc
    27181 atgatgccca gtttggtttc cggcagaccg atgcgcagat ccggcgtcgc cagacgataa
    27241 tcggtcgcca gcacgcattc gcagccacca cccagcgcat agccgttgac ggcagcaatg
    27301 gtcggcaccg gcagatcttc caggcgatta aacacgctat tggcaaagtg cagccactga
    27361 cttaactgtt cttcaggaac gaggaacagg gacaaaaatt cggtgatatc agcaccgacg
    27421 ataaaggctg ctttgttcga acgcagcagc agccctttta gatctgattg ctgttccagc
    27481 acgccgatgg cctcgccgag gctggcgacg gtcgcagtgt cgagtttatt aactgaacct
    27541 ggggcatcaa ataccagttc ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg
    27601 cctttgtaaa gcatgtcagt ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat
    27661 cggagtgtgt gttttgtgtt aaaaatatgc aaataaaaga ttaaagaaat gccgatctga
    27721 tcacgctcgg cagaaatcac gctctggatg aacgatgtgc taagatgcgg agacttaagg
    27781 tcaaaaaaac agaagggtaa aaaatggaat cactggcctc gctctataaa aatcatatag
    27841 ctaccttaca agaacggact cgcgatgcgc tggcgcgctt caagctggat gcattactta
    27901 ttcactccgg cgagctgttc aacgtttttc tcgacgatca tccctatccg tttaaagtga
    27961 acccgcaatt caaagcgtgg gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg
    28021 atggcgtgaa caagccgaaa ctgtggttct atctgccggt tgattactgg cacaacgtcg
    28081 aaccgctgcc gacctccttc tggactgaag atgtggaagt gatcgcgctg ccgaaagccg
    28141 atggcattgg tagcctgctg cctgctgcgc gcggcaatat cggttatatc ggtccggtgc
    28201 cggaacgtgc gctgcaactg ggtattgagg ccagcaacat caacccgaaa ggggtgatcg
    28261 actacctgca ttattaccgc tccttcaaaa ccgagtacga actggcctgt atgcgtgaag
    28321 cgcagaaaat ggcggtcaat ggtcatcgtg cggcagaaga agcgttccgt tctggcatga
    28381 gcgagttcga tatcaatatt gcctatctga ccgcgaccgg tcatcgtgat accgacgtac
    28441 cttacagcaa cattgtggcg cttaacgaac acgctgcggt gttgcattac accaaactgg
    28501 accaccaggc accggaagag atgcgcagct tcctgctgga tgccggggcc gaatataacg
    28561 gctatgcggc tgacctgacc cgtacctggt cggcaaaaag cgacaacgac tacgcacagc
    28621 tggtgaaaga cgtaaatgat gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca
    28681 gctatgtgga ttaccacatc cagttccatc agcgcatcgc caaactgctg cgtaaacatc
    28741 aaatcatcac cgatatgagt gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta
    28801 tgccgcacgg tattggccat ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc
    28861 aggatgatag cggtacacac ctcgcggcac cggcaaaata tccgtacctg cgctacaccc
    28921 gtattctcca gccgggcatg gtgttaacca tcgaaccggg tatctacttc attgaatcgc
    28981 ttctggcacc gtggcgtgaa gggcagttca gcaagcactt caactggcag aaaattgaag
    29041 cactgaaacc gttcggcggc attcgtatcg aagacaacgt ggtgatccac gaaaacaacg
    29101 tggaaaacat gacccgggat ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc
    29161 accggtcacg gtcgttgaag agatcaaaaa gagccgcttc ataacgatgt tggcgcatac
    29221 cgatggcgtt gaggcggcga aagcgtttgt tgaatcggtg cgggcagaac accccgatgc
    29281 ccgccaccat tgcgtggcgt gggtggcggg tgcgccggat gattctcaac agctgggttt
    29341 ctctgacgac ggggagccgg cgggaacggc aggtaaaccg atgctcgccc agctaatggg
    29401 cagcggcgtc ggggaaatta ccgctgtggt agtgcgctac tacggcggca tattgcttgg
    29461 caccggtggg ttagtgaaag cgtatggcgg cggcgtgaat caggcgctgc gccagctaac
    29521 gacccaacgc aagacgccat taaccgaata tactttgcaa tgtgaatata gtcagttaac
    29581 cggcattgaa gcgttgctgg ggcagtgtga cggcaaaatt atcaacagtg attatcaggc
    29641 attcgttctg ctgcgggtgg cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct
    29701 ggcggatttt agccgtggtt cattgcaatt gttagcgatt gaagaataat ccccacttcg
    29761 ttttgcagaa ctaaggaagc ggcagagatg cattttcgcg ccattacccg aatcgttgga
    29821 ctactggtca tcttattttc agggaccatg attatccctg ggctggtagc actcatctac
    29881 cgggatggag cgggccgcgc ttttacccag accttttttg tcgccctcgc cattggctct
    29941 atgctgtggt ggccgaaccg caaagagaag ggcgaactga aatcccgtga ggggtttctg
    30001 atagtggtgc tgttttggac cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg
    30061 gaaagcccga acctcacgat taccgatgcg ttttttgaat ctttctctgg cctgaccacc
    30121 accggggcca ctacgctggt ggggctggat tcgctccctc atgccatcct cttttatcgc
    30181 cagatgctgc aatggtttgg cgggatgggg atcatcgtgt tagcggttgc gatactgcct
    30241 atcctcggcg tgggtgggat gcagctctat cgcgcagaaa tgcccggccc gctgaaagat
    30301 aacaaaatgc gcccgcgaat tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg
    30361 ctgaccgtcg cctgtgcgct ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc
    30421 ggccatagct ttgcgactat cgctattggc ggcttctcga cacatgatgc cagtatcggt
    30481 tatttcgaca gtccgactat taacactatc attgctatct tcctgctgat ctccggctgt
    30541 aactacggtc tgcacttttc actgttaagt gggcgtagtc tgaaggttta ttggcgcgat
    30601 ccggaatttc gcatgtttat cggcgtacag tttacgctgg tggttatttg tacactcgta
    30661 ctgtggtttc ataatgtcta cagttcggcg ctgatgacaa ttaaccaggc gtttttccag
    30721 gtggtatcga tggcgacaac cgccgggttt acgactgaca gcattgcccg ctggccgctc
    30781 tttttgccgg tactgctttt atgttcagct tttatcggcg gttgcgccgg gtcaacgggc
    30841 ggtggcctga aagtgatccg catcctgctg ctgtttaagc aggggaaccg tgagctgaaa
    30901 cgactggtgc atccgaacgc cgtctatagc attaagctgg ggaatcgcgc actgccggaa
    30961 cgtatcctcg aagccgtttg gggatttttc tccgcctatg cattggtgtt tattgtcagt
    31021 atgctggcga ttatcgccac gggcgtggat gatttttctg cctttgcctc agttgttgcg
    31081 acattgaata acctggggcc agggcttggc gtggttgctg ataactttac cagtatgaac
    31141 ccggtggcta aatggatcct gattgccaac atgctgtttg gtcgtctcga ggtctttaca
    31201 ttactggtgc tctttacccc gactttctgg cgtgaatgat ggagtaatac gtgaaaacat
    31261 taattctttt ctcaacaagg gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg
    31321 aactgaaaga actggggatc cagacggatg tcgccaatgt gcaccgcatt gaagaaccac
    31381 aatgggaaaa ctatgaccgt gtggtcattg gtgcttctat tcgctatggt cactaccact
    31441 ctgcgttcca ggaatttgtc aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct
    31501 tttattccgt aaacctggtg gcgcgcaaac cggagaagcg tactccacag accaacagct
    31561 acgcgcggaa gtttctgatg aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg
    31621 gggcgctgcg ttacccacgt tatcgctggt acgaccgttt tatgctcaag ctgattatga
    31681 agatgtcagg cggtgaaacg gatacgcgca aagaagttgt ctataccgat tgggagcagg
    31741 tggcgaattt cgcccgagaa atcgcccatt taaccgacaa accgacgctg aaataagcat
    31801 aaagaataaa aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat
    31861 aactccctat aatgcgccac cactgacacg gaacaacggc aaacacgcag ccgggtcagc
    31921 ggggttctcc tgagaattcc gacagagaaa gcaaaaataa atgcttgact ttgtagcggg
    31981 aaagcgtatt atgaacaccc cgcgc
//
LOCUS       DAZPDE010000029        31187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPDE010000029 DAZPDE010000000
VERSION     DAZPDE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.029"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHU0J_001260"
     CDS             389..2047
                     /locus_tag="ACHU0J_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1216020.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHU0J_001261"
     CDS             complement(2044..3000)
                     /locus_tag="ACHU0J_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1216021.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHU0J_001262"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHU0J_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1216022.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHU0J_001263"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHU0J_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1216023.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHU0J_001264"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHU0J_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1216024.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHU0J_001265"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHU0J_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1216025.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHU0J_001266"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHU0J_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1216026.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHU0J_001267"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHU0J_001267"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1216027.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHU0J_001268"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHU0J_001268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHU0J_001269"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHU0J_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1216028.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHU0J_001270"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHU0J_001270"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1216029.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHU0J_001271"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHU0J_001271"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1216030.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHU0J_001272"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHU0J_001272"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1216031.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHU0J_001273"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHU0J_001273"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1216032.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHU0J_001274"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHU0J_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1216033.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHU0J_001275"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHU0J_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1216034.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHU0J_001276"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHU0J_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1216035.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHU0J_001277"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHU0J_001277"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1216036.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHU0J_001278"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHU0J_001278"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1216037.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHU0J_001279"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHU0J_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHU0J_001280"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHU0J_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1216038.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHU0J_001281"
     CDS             28042..28833
                     /locus_tag="ACHU0J_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR1216039.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHU0J_001282"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHU0J_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1216040.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHU0J_001283"
     CDS             29810..30958
                     /locus_tag="ACHU0J_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216041.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31187
                     /locus_tag="ACHU0J_001284"
     CDS             30976..>31187
                     /locus_tag="ACHU0J_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1216042.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7690 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagga
//
LOCUS       DAZPDE010000030        30666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPDE010000030 DAZPDE010000000
VERSION     DAZPDE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.030"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..588
                     /locus_tag="ACHU0J_001285"
     CDS             <1..588
                     /locus_tag="ACHU0J_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR1216043.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACHU0J_001286"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACHU0J_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1216044.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACHU0J_001287"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACHU0J_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1216045.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACHU0J_001288"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACHU0J_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1216046.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACHU0J_001289"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACHU0J_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1216047.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHU0J_001290"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHU0J_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1216048.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACHU0J_001291"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACHU0J_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1216049.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHU0J_001292"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHU0J_001292"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1216050.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHU0J_001293"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHU0J_001293"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1216051.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHU0J_001294"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHU0J_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1216052.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHU0J_001295"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHU0J_001295"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1216053.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHU0J_001296"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHU0J_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1216054.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHU0J_001297"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHU0J_001297"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1216055.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHU0J_001298"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHU0J_001298"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1216056.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHU0J_001299"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHU0J_001299"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1216057.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHU0J_001300"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHU0J_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR1216058.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACHU0J_001301"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACHU0J_001301"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1216059.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACHU0J_001302"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACHU0J_001302"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1216060.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACHU0J_001303"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACHU0J_001303"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1216061.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACHU0J_001304"
     CDS             22192..22782
                     /locus_tag="ACHU0J_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1216062.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACHU0J_001305"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACHU0J_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1216063.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACHU0J_001306"
     CDS             23254..23949
                     /locus_tag="ACHU0J_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1216064.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHU0J_001307"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHU0J_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1216065.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACHU0J_001308"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACHU0J_001308"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1216066.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACHU0J_001309"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACHU0J_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1216067.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHU0J_001310"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHU0J_001310"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1216068.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHU0J_001311"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHU0J_001311"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1216069.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7190 a         7982 c         8440 g         7054 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgattg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAZPDE010000031        30132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPDE010000031 DAZPDE010000000
VERSION     DAZPDE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.031"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHU0J_001312"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHU0J_001312"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1216070.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHU0J_001313"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHU0J_001313"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1216071.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHU0J_001314"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHU0J_001314"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1216072.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHU0J_001315"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHU0J_001315"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1216073.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHU0J_001316"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHU0J_001316"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1216074.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHU0J_001317"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHU0J_001317"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1216075.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHU0J_001318"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="ACHU0J_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1216076.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHU0J_001319"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="ACHU0J_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1216077.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHU0J_001320"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="ACHU0J_001320"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1216078.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHU0J_001321"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="ACHU0J_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1216079.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHU0J_001322"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="ACHU0J_001322"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1216080.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="ACHU0J_001323"
     CDS             complement(15666..16703)
                     /locus_tag="ACHU0J_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1216081.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHU0J_001324"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="ACHU0J_001324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1216082.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHU0J_001325"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="ACHU0J_001325"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1216083.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="ACHU0J_001326"
     CDS             19445..19885
                     /locus_tag="ACHU0J_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1216084.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHU0J_001327"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="ACHU0J_001327"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1216085.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21545)
                     /locus_tag="ACHU0J_001328"
                     /pseudo
     CDS             complement(20612..21545)
                     /locus_tag="ACHU0J_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHU0J_001329"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="ACHU0J_001329"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1216086.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHU0J_001330"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="ACHU0J_001330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1216087.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHU0J_001331"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="ACHU0J_001331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR1216088.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHU0J_001332"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="ACHU0J_001332"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1216089.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHU0J_001333"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="ACHU0J_001333"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1216090.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHU0J_001334"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="ACHU0J_001334"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1216091.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHU0J_001335"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="ACHU0J_001335"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1216092.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHU0J_001336"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="ACHU0J_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1216093.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7272 a         8411 c         7608 g         6841 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcaacgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt tt
//
LOCUS       DAZPDE010000032        29261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPDE010000032 DAZPDE010000000
VERSION     DAZPDE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.032"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHU0J_001337"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHU0J_001337"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1216094.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHU0J_001338"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHU0J_001338"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1216095.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHU0J_001339"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHU0J_001339"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1216096.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHU0J_001340"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHU0J_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1216097.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHU0J_001341"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHU0J_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1216098.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHU0J_001342"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHU0J_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1216099.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHU0J_001343"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHU0J_001343"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1216100.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHU0J_001344"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHU0J_001344"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1216101.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHU0J_001345"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHU0J_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1216102.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHU0J_001346"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHU0J_001346"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1216103.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHU0J_001347"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHU0J_001347"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1216104.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHU0J_001348"
     CDS             8212..9075
                     /locus_tag="ACHU0J_001348"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1216105.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHU0J_001349"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHU0J_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1216106.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHU0J_001350"
     CDS             10411..11028
                     /locus_tag="ACHU0J_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1216107.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHU0J_001351"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHU0J_001351"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1216108.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHU0J_001352"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHU0J_001352"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1216109.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHU0J_001353"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHU0J_001353"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1216110.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHU0J_001354"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHU0J_001354"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1216111.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHU0J_001355"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHU0J_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1216112.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHU0J_001356"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHU0J_001356"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1216113.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHU0J_001357"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHU0J_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1216114.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHU0J_001358"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHU0J_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1216115.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHU0J_001359"
     CDS             21859..23568
                     /locus_tag="ACHU0J_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1216116.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHU0J_001360"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHU0J_001360"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1216117.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHU0J_001361"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHU0J_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHU0J_001362"
     tRNA            27625..27719
                     /locus_tag="ACHU0J_001362"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHU0J_001363"
     CDS             27991..29202
                     /locus_tag="ACHU0J_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1216118.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7231 a         7840 c         7342 g         6848 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac a
//
LOCUS       DAZPDE010000033        29084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPDE010000033 DAZPDE010000000
VERSION     DAZPDE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.033"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHU0J_001364"
     CDS             <1..155
                     /locus_tag="ACHU0J_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1216119.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHU0J_001365"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHU0J_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1216120.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHU0J_001366"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHU0J_001366"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1216121.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHU0J_001367"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHU0J_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHU0J_001368"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHU0J_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1216122.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHU0J_001369"
     CDS             complement(5626..7134)
                     /locus_tag="ACHU0J_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1216123.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHU0J_001370"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHU0J_001370"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1216124.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHU0J_001371"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHU0J_001371"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1216125.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHU0J_001372"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHU0J_001372"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1216126.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHU0J_001373"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHU0J_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1216127.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHU0J_001374"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHU0J_001374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1216128.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHU0J_001375"
     CDS             complement(14283..15602)
                     /locus_tag="ACHU0J_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR1216129.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHU0J_001376"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHU0J_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1216130.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHU0J_001377"
     CDS             16699..17034
                     /locus_tag="ACHU0J_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1216131.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHU0J_001378"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHU0J_001378"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1216132.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHU0J_001379"
     CDS             complement(17790..19172)
                     /locus_tag="ACHU0J_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1216133.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHU0J_001380"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHU0J_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1216134.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHU0J_001381"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHU0J_001381"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1216135.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHU0J_001382"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHU0J_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1216136.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHU0J_001383"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHU0J_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1216137.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHU0J_001384"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHU0J_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1216138.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHU0J_001385"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHU0J_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1216139.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHU0J_001386"
     CDS             complement(25947..26768)
                     /locus_tag="ACHU0J_001386"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1216140.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHU0J_001387"
     CDS             complement(26868..26951)
                     /locus_tag="ACHU0J_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216141.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHU0J_001388"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHU0J_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1216142.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHU0J_001389"
     CDS             27623..27727
                     /locus_tag="ACHU0J_001389"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1216143.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHU0J_001390"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHU0J_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         7467 a         6926 c         7421 g         7270 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaa
//
LOCUS       DAZPDE010000034        28484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPDE010000034 DAZPDE010000000
VERSION     DAZPDE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.034"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1170
                     /locus_tag="ACHU0J_001391"
     CDS             <1..1170
                     /locus_tag="ACHU0J_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1216144.1"
                     /translation="SYKPIWNWEISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTS
                     TRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQ
                     LDTNVNTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIID
                     LLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYK
                     IMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTS
                     RAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGL
                     NARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1360..1473
                     /gene="yhiY"
                     /locus_tag="ACHU0J_001392"
     CDS             1360..1473
                     /gene="yhiY"
                     /locus_tag="ACHU0J_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1216145.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1536..2603
                     /locus_tag="ACHU0J_001393"
     CDS             1536..2603
                     /locus_tag="ACHU0J_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1216146.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2600..5335
                     /gene="rbbA"
                     /locus_tag="ACHU0J_001394"
     CDS             2600..5335
                     /gene="rbbA"
                     /locus_tag="ACHU0J_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1216147.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5335..6459
                     /locus_tag="ACHU0J_001395"
     CDS             5335..6459
                     /locus_tag="ACHU0J_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1216148.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6518..6793
                     /locus_tag="ACHU0J_001396"
     CDS             6518..6793
                     /locus_tag="ACHU0J_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1216149.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            6790..7149
                     /locus_tag="ACHU0J_001397"
     CDS             6790..7149
                     /locus_tag="ACHU0J_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1216150.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7269..7670)
                     /gene="nikR"
                     /locus_tag="ACHU0J_001398"
     CDS             complement(7269..7670)
                     /gene="nikR"
                     /locus_tag="ACHU0J_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1216151.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7676..8482)
                     /gene="nikE"
                     /locus_tag="ACHU0J_001399"
     CDS             complement(7676..8482)
                     /gene="nikE"
                     /locus_tag="ACHU0J_001399"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1216152.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8479..9243)
                     /gene="nikD"
                     /locus_tag="ACHU0J_001400"
     CDS             complement(8479..9243)
                     /gene="nikD"
                     /locus_tag="ACHU0J_001400"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1216153.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9243..10076)
                     /gene="nikC"
                     /locus_tag="ACHU0J_001401"
     CDS             complement(9243..10076)
                     /gene="nikC"
                     /locus_tag="ACHU0J_001401"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1216154.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10073..11017)
                     /gene="nikB"
                     /locus_tag="ACHU0J_001402"
     CDS             complement(10073..11017)
                     /gene="nikB"
                     /locus_tag="ACHU0J_001402"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1216155.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11017..12591)
                     /gene="nikA"
                     /locus_tag="ACHU0J_001403"
     CDS             complement(11017..12591)
                     /gene="nikA"
                     /locus_tag="ACHU0J_001403"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1216156.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12702..13289)
                     /gene="acpT"
                     /locus_tag="ACHU0J_001404"
     CDS             complement(12702..13289)
                     /gene="acpT"
                     /locus_tag="ACHU0J_001404"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1216157.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13344..14393)
                     /locus_tag="ACHU0J_001405"
     CDS             complement(13344..14393)
                     /locus_tag="ACHU0J_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1216158.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14527..15744
                     /locus_tag="ACHU0J_001406"
     CDS             14527..15744
                     /locus_tag="ACHU0J_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216159.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15748..16305)
                     /gene="dcrB"
                     /locus_tag="ACHU0J_001407"
     CDS             complement(15748..16305)
                     /gene="dcrB"
                     /locus_tag="ACHU0J_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1216160.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16378..17043)
                     /locus_tag="ACHU0J_001408"
     CDS             complement(16378..17043)
                     /locus_tag="ACHU0J_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1216161.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17264..17509
                     /gene="tusA"
                     /locus_tag="ACHU0J_001409"
     CDS             17264..17509
                     /gene="tusA"
                     /locus_tag="ACHU0J_001409"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1216162.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17611..19809)
                     /gene="zntA"
                     /locus_tag="ACHU0J_001410"
     CDS             complement(17611..19809)
                     /gene="zntA"
                     /locus_tag="ACHU0J_001410"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1216163.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19883..20509)
                     /locus_tag="ACHU0J_001411"
     CDS             complement(19883..20509)
                     /locus_tag="ACHU0J_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1216164.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20650..21009
                     /locus_tag="ACHU0J_001412"
     CDS             20650..21009
                     /locus_tag="ACHU0J_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1216165.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21012..21281)
                     /locus_tag="ACHU0J_001413"
     CDS             complement(21012..21281)
                     /locus_tag="ACHU0J_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1216166.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21271..21867)
                     /gene="rsmD"
                     /locus_tag="ACHU0J_001414"
     CDS             complement(21271..21867)
                     /gene="rsmD"
                     /locus_tag="ACHU0J_001414"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1216167.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            22017..23513
                     /gene="ftsY"
                     /locus_tag="ACHU0J_001415"
     CDS             22017..23513
                     /gene="ftsY"
                     /locus_tag="ACHU0J_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1216168.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23516..24184
                     /gene="ftsE"
                     /locus_tag="ACHU0J_001416"
     CDS             23516..24184
                     /gene="ftsE"
                     /locus_tag="ACHU0J_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1216169.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24177..25235
                     /gene="ftsX"
                     /locus_tag="ACHU0J_001417"
     CDS             24177..25235
                     /gene="ftsX"
                     /locus_tag="ACHU0J_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1216170.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25480..26334
                     /gene="rpoH"
                     /locus_tag="ACHU0J_001418"
     CDS             25480..26334
                     /gene="rpoH"
                     /locus_tag="ACHU0J_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1216171.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26605..27708
                     /gene="livJ"
                     /locus_tag="ACHU0J_001419"
     CDS             26605..27708
                     /gene="livJ"
                     /locus_tag="ACHU0J_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1216172.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27907..28290)
                     /gene="panM"
                     /locus_tag="ACHU0J_001420"
     CDS             complement(27907..28290)
                     /gene="panM"
                     /locus_tag="ACHU0J_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1216173.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6811 a         7341 c         7844 g         6488 t
ORIGIN      
        1 tcgtacaaac ccatctggaa ctgggaaata tcgcaataca atgtatttga tattaaattc
       61 gaaacaatta taaaacccca gttcgctgac agaacctatt tctccaattg ttcacctgtt
      121 gatcccacaa gtaccaggcc gacctatttt ggcgataccg atggctcagt cggtgcagtt
      181 ttgtacgcgc tttttgcaac aggacatttg ggaatcatgg ctgaaggaga aaatttcctt
      241 agccagttgc ttaatataga agatgaagtt ctcaacgtat tgctaaggga gaactttaat
      301 gagcaacttg acactaatgt taatacaata atcagtatac tgaacagaag agataatgtt
      361 ttagaaagtc ttcagcctta tttggttatt aataaagacg cagtcacgcc atgtacgttt
      421 cttggcgacc aaaccggcga tcgatttagt aatatctgtg gcgatcaatt tatcatcgat
      481 ttgttaaaac gcataatgag tatcaatgaa aatgttcatg tattagctgg aaatcatgaa
      541 acgaattgca atggtaacta tatgcaaaac ttcacgcgca tgaaaccact tgatgaggat
      601 acctacgacg ggataaaaga ttatcctgta tgtttctatg accccaaata caaaataatg
      661 gcaaatcatc atgggataac ttttgatgat cagcgaaaac gttacattat aggaccgata
      721 actgtctcta ttgatgaaat gaccaatgct ctcgatccgg tcgaattagc ggaaattatt
      781 aataaaaagc atcatgctat aattaatggc aaaaagttta aaactagccg ggctatctct
      841 tgtcgatcct ttaatcgtta tttttctgtt tcgacagatt acagacctaa acttgaagca
      901 ctattagcat gttcacaaat gttaggtata aatcaagttg ttgcacataa cggaaatggt
      961 gggagagaac gcataggtga aacggggaca gttttaggtc ttaacgcccg tgacagtaaa
     1021 catgctggaa gaatgttcag catgcataat tgccaaatta accctggtgc agggccagag
     1081 ataaccactc cctggaagtc ttaccagcat gaaaaaaaca gaaatggact tatgcctttg
     1141 attagaaggc gcacaatgtt acagctctga accatagaca acatgaatat taatgttact
     1201 atctggttga gactgttgat aaaacgtaaa aaggaatgct ctgttcctgc aactgaacag
     1261 gcgtgaaatg cgatccaaca ggatggatac cgttttgtca gccagtttta tagatcacac
     1321 ttatgagtat gcgtgtttca tttacggcca ggaatgctga tgatgacaac attactaccc
     1381 gttttcacta agccttcccc gttggcgctc aatgctctgc gcgctggtcg tatttgtcgt
     1441 ttccttctta tcccggatgg aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg
     1501 cgtcgtttgc ttacagggat tatggcgggt cgattatgga taagagtaag cgccatctgg
     1561 cgtggtgggt tgtcggggca ctggcggtgg ctgccgtcgt ggcgtggtgg ctgttgcgcc
     1621 cggcgggtgt gccggaaggc tttgctgtca gcaatgggcg cattgaagcg acggaagtgg
     1681 atatcgccag caaaattgcc gggcgtatcg acaccattct ggtgaaagaa ggccagtttg
     1741 ttcgcgaagg cgaagtgctg gcgaagatgg atactcgcgt gttgcaggaa cagcgactgg
     1801 aagccatcgc gcaaatcaaa gaggcacaaa gcgccgttgc cgccgcgcag gctttgctgg
     1861 agcaacgaca aagtgaaact cgtgccgcac agtcgctggt caatcaacgc caggccgaac
     1921 tggactctgt tgccaaacgt catacgcgtt cccgctcgct cgcccaacga ggggctattt
     1981 ctgcgcaaca gctggatgac gaccgcgccg ccgctgagag cgcccgagct gcgctggaat
     2041 cagcgaaagc ccaggtttcg gcttctaaag cggctataga agcagcacgc accaatatca
     2101 ttcaggcgca aactcgggtc gaagcagcgc aagccactga acggcgcatt gccgcagata
     2161 tcgatgacag tgaactgaaa gccccgcgtg acggacgcgt gcagtatcgg gttgccgagc
     2221 caggggaagt gctggcggca ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct
     2281 atatgacctt cttcctgcca accgaacagg cgggcacgct gaaactgggc ggtgaagccc
     2341 ggctgatcct cgatgccgcg ccagatctgc gtattcctgc aaccatcagt tttgtcgcca
     2401 gtgtcgccca gttcacgcca aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt
     2461 tccgcgtcaa agcgcgtatc ccaccggaat tactccagca gcatctggaa tatgtcaaaa
     2521 ccggtttacc gggcgtagcg tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc
     2581 tcgtggtgag gttgccgcaa tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca
     2641 actggcgggc gtgagccagc attatggaaa aaccgttgcg ctgaacaata tcactctcga
     2701 tattcaggcc cgttgcatgg tcgggctgat tggtccggat ggcgtcggga agtcgagttt
     2761 attgtcactg atttccggtg cccgcgtcat tgagcagggc aatgtggtgg tgctgggtgg
     2821 cgatatgcac gacccgaagc atcgccgaga cgtctgcccg cgcatcgcct ggatgccgca
     2881 ggggctgggc aaaaacctct accacacctt gtcggtgtat gaaaatgtcg attttttcgc
     2941 tcgcctgttc ggtcacgata aagcggagcg ggaagcgcga attaatgagc tgctgaccag
     3001 caccgggtta gcaccgtttc gcgatcgtcc ggcaggtaaa ctctccggcg ggatgaagca
     3061 aaaacttggg ctgtgctgcg cgttaatcca cgacccggaa ctgttgatcc ttgatgagcc
     3121 aacaacgggg gttgacccgc tctcccgcgc ccagttctgg gatctgatcg acagtattcg
     3181 ccagcggcag agcaatatga gcgtgctggt cgccaccgcc tatatggaag aggccgaacg
     3241 cttcgactgg ctggtagcga tgaatgccgg agaagtgctg gcaaccggca gcgccgaaga
     3301 gctgcggcag caaacgcaaa gcgccacgct ggaagaagca tttattaatc tgttaccgca
     3361 agcgcaacgc caggcgcatc aggcggtagt gatcccaccg tatcaacctg aaaacgcaga
     3421 gattgccatc gaagcgcgcg atctgaccat gcgttttggt tccttcgttg ccgttgatca
     3481 cgtcaatttc cgcattccac gcggggagat ttttggattc cttggttcga acggctgcgg
     3541 taaatccacc accatgaaaa tgcttaccgg actgctgccc gccagcgaag gtgaggcgtg
     3601 gctatttggg caaccggttg atccaaaaga tatcgatacc cgccgtcgtg tgggctatat
     3661 gtcgcaggcg ttttcgctct ataacgaact caccgtgcgg caaaacctgg agttacatgc
     3721 ccgtttgttt cacatcccgg aagcggaaat tcccgcgcga gtggctgaaa tgagcgagcg
     3781 ttttaagctc aacgacgttg aagatgttct gccggagtca ttgccgctcg gcattcgcca
     3841 gcggctttcg ctggcggtgg cggtgattca tcgcccggag atgttaatcc tcgatgagcc
     3901 tacttctggt gtcgatccgg tggcgaggga tatgttctgg cagttgatgg tcgatctctc
     3961 gcgccaggac aaagtgacta tcttcatctc cacccacttt atgaacgaag cggaacgttg
     4021 cgaccgcatc tcactgatgc acgccggaaa agtgcttgcc agcggtacac cgcaggaact
     4081 ggttgagaaa cgcggagccg ccagtctgga agaggcattt atcgcctatt tgcaggaagc
     4141 ggcagggcag agcaacgaag ccgaagcgcc gcccgtggta cacgacacca cccacgcgcc
     4201 gcgtcaggga tttagcctgc gccgtctgtt tagctacagc cgccgcgaag cgctggaact
     4261 gcgacgcgat ccagtacgtt cgacgctggc gctgatggga acggtgatcc tgatgctgat
     4321 aatgggttac ggcatcagta tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga
     4381 ccagaccgtc agtagccagg cgtggacact caacctctcc ggttcccgtt actttatcga
     4441 acagccgccg ctcaccagtt atgacgagct tgatcgtcgg atgcgtgcgg gcgatatcac
     4501 ggtggccatt gagatcccgc ccaatttcgg gcgcgatatc gcgcgtggta cgcctgtgga
     4561 actcggcgtc tggatcgacg gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt
     4621 gcaggcaatg caccagagtt ggcttcagga tgtggcgagc cgacaatcga cacccgccag
     4681 ccaaagcggg ctgatgaaca ttgagacgcg ttatcgctat aacccggacg taaaaagcct
     4741 gccagcgatt gttccggcgg tgatcccgct tctgctgatg atgatcccgt caatgctaag
     4801 cgcccttagc gtggtgcggg aaaaagagct agggtcgatt atcaaccttt acgtgacccc
     4861 caccacccgt agcgaatttt tgcttggcaa acagctgccg tacatcgcgc tggggatgct
     4921 gaactttttc ctgctctgcg gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag
     4981 tttcctgacg ctcaccttgg cggcgctgct gtatatcatc attgccactg gaatggggct
     5041 gctgatctcc acctttatga aaagccagat tgccgccatt ttcggaacgg cgattatcac
     5101 attgatcccg gcgacacagt tttccgggat gatcgatccg gtagcttcgc tggaagggcc
     5161 tggacgttgg atcggcgagg tttacccgac cagtcatttt ctgactatcg cccgcggaac
     5221 gttctcgaaa gcgctggatc tgactgattt gtggcaactt tttatcccgt tgctgatagc
     5281 catcccgctg gtgatgggct taagcatcct gctgctgaaa aaacaggagg gatgatgcgc
     5341 catttacgca atatttttaa tctgggtatc aaagagttgc gcagtctgct cggtgataaa
     5401 gcgatgctga cgctgattgt cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt
     5461 acgccaggat cgttgaacct cgcgccgatc gccattgccg atatggatca atcgcagtta
     5521 tcgaaccgga tcgttaacag cttctatcgt ccgtggtttt tgccaccgga gatgatcacc
     5581 gccgatgaga tggatgccgg actggacgcc ggacgctata ccttcgcgat aaatattccg
     5641 cctaattttc agcgtgatgt cctcgccgga cgccagccgg atattcaggt gaacgtcgat
     5701 gccacgcgca tgagccaggc atttaccggc aatgggtata tccagaatat tatcaacggt
     5761 gaagtgaaca gctttgtcgc gcgctaccgt gataacagcg aaccgttggt atcgctggaa
     5821 acccggatgc gctttaaccc gaaccttgat cccgcgtggt ttggcggggt gatggcgatc
     5881 atcaacaaca ttaccatgct ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt
     5941 gaacacggca cggtggaaca tttactggtg atgccgataa cgccgtttga gatcatgatg
     6001 gcgaagatct ggtcgatggg gctggtggtg ctggtggtat cgggattatc gctggtgctg
     6061 atggtgaaag gtgtactggg cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc
     6121 gtggcgctca gtctgtttgc caccacgtca atcggcattt ttatggggac gatagcgcgt
     6181 tcaatgccgc aactggggct gctggtgatt ctggtgctgc tgccgctgca aatgctttcc
     6241 ggtggatcca cgccgcgcga aagtatgccg cagatggtgc aggacattat gctgaccatg
     6301 ccgacgacac actttgttag cctcgcgcag gccatcctct accggggtgc cggattcgaa
     6361 atcgtctggc cgcagttcct gacgctgatg gcaattggcg gcgcgttttt caccatcgca
     6421 ctgctgcgat tcaggaagac gattgggaca atggcgtaag gaagggaaac ttgacttcat
     6481 actgtgatgt cataaagtga agtcataagg acatgccatg agaaccaagg tacaggcttt
     6541 acggaagaaa caaaaaaata cattggatca aatatttaaa actcctgttc ctcaagggat
     6601 caagtggtct gatatagagt cactggttaa agcattaggc ggagaaatta aggaaggaag
     6661 aggttcgcgt tgcaagttca tactaaatat gagcgttgcg tgtttccatc ggcctcatcc
     6721 gtcgccagat accgataaag gcgctgtaga aagcgtgtgt gactggttac taagtatagg
     6781 ggtaaaacca tgatcaaact gaagacgcca aactctatgg agattgccgg gcaaccagct
     6841 gttattacct acgtgccaga actgaatgca tttcgtggca aattcctggg cttgtcaggt
     6901 tattgtgatt ttgtctctga cagtattcag gggctgcaaa aagaaggaga gctttccctg
     6961 cgagagtatc ttgaggattg taaagctgcg ggtattgagc cgtatgcgcg tacagaaaag
     7021 attaagacct ttacgttacg ctatccagaa tcgctgagtg aacgccttaa taatgcggct
     7081 gcgcagcaac aagtctctgt taatacctat attattgaaa cactgaatga gcgtttgaat
     7141 catttataaa ttttagcgac gtacattacg cagaatatct gcctttacag gatctatcta
     7201 tgcaactgaa cttaatttta taaattaagt tcagttaata tcttttcttt ccttgtttta
     7261 gaaataactc aatcttcctt cggcaggcac tgcaaatgcc cgtgccgcac gccgcgctgg
     7321 gcgataacgt catcggcaaa atgctgcacg tcacccatgt cacctttcaa cacggcgatt
     7381 tccagacagt cgtcgtggtt gatgtgcaca tgtagcgtgg cgacggagag gtcgtggtga
     7441 tgatgctggg tggaaacaat acggctggct aagtcgcgtt tttcgtgttc atacacatac
     7501 gacagcaccg cgaaaccttg tgtgccgtgc tgctgggtag cttcctgcgc cagggcacta
     7561 cgcagaatat cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg
     7621 tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat tggcgttaaa
     7681 ccttttctga ggtgcgacgg cgcacgggga atgcgggtaa taccgcgttt tgtagcacac
     7741 gtccggcgtc agaggaaaag gttaatttat ctcccaccgc ctgggtttcg gcgatttgtc
     7801 cgttgtccat caccatcact cgctggcaaa agcgttccac caggcgtaag tcatgagtga
     7861 tgaacaggca ggcagtgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg
     7921 cctgtaacac gagatcaagg ttagaaacgg cttcatccag aatcagcaat ttcggttcga
     7981 ccgccagcgc gcgtgccagg cagacgagct ggagctggcc gccgcttaac tgcggtgggc
     8041 gtttgtcgag aacgctgtca tcgagatcga ccgccttcag catttcgctg gcgcgcgcca
     8101 gttgttcgga ttttttcagt gatagcaggt ggcgcatcgg ttcacgcagg atctcgcgca
     8161 cggttttgcg cggattcacg gcactgatgg agtcctgaaa caccatctga atatcacggc
     8221 ggaaggcttt acgttgggcg cgattaagtt tcgccagcga ttcgccgtgc cagctaatat
     8281 tcccttgtga gggagactct aagcctacca gcagccgcgc gagggtgctt tttccgcagc
     8341 cgctgcgtcc cagcagggcg acggtttcgc cgcttttcag ggtcagggaa acgttattca
     8401 gcaccgcctg atgttgatgt tttccgctaa atccaccgtg ggcatagtga tgggaaaggc
     8461 cgcagacgtt aagtaaggtc atgatgccag ctccataccg tagagggcga gatgagcgga
     8521 aaccaggctg cgcgtcaccg tatgtttggg ggcgttaaac agcgtttcta catcgccctg
     8581 ttcgacaatt ttaccttgtg acatcaccgc cacgtcatcc gccagacgcg ccacaacgcc
     8641 catatcatgg gtgaccagca gcattcccgg cgcttgtttt tgcataatgc tttccagcag
     8701 atcgaggatg cgcgcctgtg ctaccacgtc gaggtcggtg gtcggttcat cggcgatgat
     8761 aaacggtgat tcacacagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct
     8821 catctcgaac gggtacagct tcagcacgcg cgcggcgttt tccagcccca ccgcttctat
     8881 ggcagcggta agcgtagcgt catcggcggg tttccctaac gccaggcagg tttcccgcgc
     8941 gtgggtgtgc atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc
     9001 aattttgatg ccgcgcaggg cgtaaggcga aaccggtttg ccatcggcta aaatttcccc
     9061 cgccgtctgg cgaacgccag cgggcaaaat gcccagcgtc gcggcgcagg ttaatgattt
     9121 cccgctgccg ctaccgccga ctaacgccag cacgcgcccg cgttgcaggg ttaacgatac
     9181 accgtgtacc agcggctgcg cggcctgtag cgtgatatta cgtagttcaa tctgttgcgg
     9241 cattagtgtg cgtgctccgt caccagatga ggatccagat gatcgcgcag tgcgtcaccc
     9301 accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc
     9361 ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc
     9421 gcggtcacac cgaggccaag gaaagacatc cccgcgacgt gcagcatcat atggccgata
     9481 tccagcgttg ccagcaccag cagcgaaggg atcaccgcgc ctgccagatg atcgacaaac
     9541 acccgcacat ggcccgcacc ggaaagccgt gacgccagca caaactcgcg ttggcgtagt
     9601 gaaatcacca agctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg
     9661 ataattacgt tggtcagccc ggtgccgagc acgccaacca taaagaacga cagaatcgag
     9721 gtcgggaagg tcataaacat atcggcgacg cgcatggtgg cctgatcaac gtgcccgcca
     9781 atcaacccgg cgctgccgcc aataaccagc cctaatgtca gcaccagcag caggcaggcc
     9841 attaccgaac cgagcgacac gcgggtcgct gccatcagcc gcgagaaaat atcgcgacct
     9901 aagtgatcgg tgcccagcca gtgctgcgca tccggcgaaa gcaggcacga cggcaaatca
     9961 attgcctgtg gatcgtacgg ctgccaccac tggctggtga gcgcaatcag cgccagcagg
    10021 gcgataatga tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt
    10081 gcgctccttc atgacgacga atgcgcgggt ccagcgcggc gttgagcaaa tcgacaatca
    10141 aattacagac cacaaaaacc accaccatca tcagcgtaaa gcactgaatc accggatagt
    10201 cacggttaaa aatcgccgac accgcatagc gcccgacgcc aggccaggca aagatgtttt
    10261 cgataatcat cgtcccgcca atcaattcgc caatgtgcat ccccacggcg gtgatcatcg
    10321 gcagcgaggc attgcgcaga atatggcggc gttcggtctg tttgtcattc aggccgcgta
    10381 gccgcgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagcagtc
    10441 gcgcgttaat cgccagcgac ataaaggcga tggaaaccgc aggtaaaatg atgtgctgcc
    10501 agctgccgta gcccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcactagca
    10561 aaaatgccag ccagaagttg ggcatcgaca cgccaagaaa cgcgataaag cgcacggcga
    10621 aatccggcaa acgatcgcgg tggcgcgccg cccagatacc gagcggtacg gaagtgagca
    10681 gaattaatac cagcgccgca cctgcaagtt ccagcgtggc gggcaggaag ttcagcatat
    10741 cgtccagtac cgggcgttgg ctggcgaatg agataccaaa gtcaagatgc agcgccttcc
    10801 acaaccaggt accgtactgg acgtacagcg gctgatccag cccgagcatg gtgcgggtag
    10861 aggccagcat ctccggcgtc ggcggcaggt tagataaacg cagataatcg agcgccgggt
    10921 cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga
    10981 tcagcagcag aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg
    11041 ttcgaacgga atttcggtgg cgatcggcgc gtaggggata ttacccagct ccggttttga
    11101 taccaccatc atcgagatgt agctgatagg cagataaacc gcctcgtcat gcagacgggt
    11161 cagaatgtcg cgatacagcg cctgacgttg cgtttcgtca tgggtcgcca gcacttcgcc
    11221 gatctcttta tcaatcagcg gcttgtcggc taatccttgc tgtgcctgga agtcagcgtg
    11281 cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg
    11341 gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca tagatactgc tctcttcttc
    11401 gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat
    11461 cgatttgctt aacgcatcgg tgccgatgaa cgacagttca atgcgcagcg gctgaccatt
    11521 tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaactcttt
    11581 cgctttctgc ggatcgtact ggcgcggttt caggccgaga ttagcgtaag gcacagaagg
    11641 ggcaaacagg gtgtcggcga cctgctgggt gccatataac gcgttatcaa tcagcgattt
    11701 tttgtttacc gcgtaattaa gagcttcacg tactgccagc tcgttggtgg gggctttggc
    11761 ggtattgagc gccagcatca cggtttcgat cggctgtgac agttgggtgt ggtaagccgg
    11821 attctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag
    11881 gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtccggga tgacgttaaa
    11941 ggtgatcttt ttaatcgctg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc
    12001 gtactgattt agtttcgatt cctgcaaaat ccacggtccg gtgccaatcg gcgctttaat
    12061 tccgttcatg gtttcatggt ttttaaactg cgagggagcg ataaaacgga aaggacgggg
    12121 cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtaattt gcagctctgt
    12181 tttattgagt gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg
    12241 acggttatcg agcactgcgc ggaagttttc tgccgccgcc tcggcatcga acggttcacc
    12301 gttggagaat ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg
    12361 agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg
    12421 ttcataaacc atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg
    12481 cccgacattc accggccagg cggtggtgat ttcatctggt gcagcggcat ggacgataaa
    12541 agacgcacaa gccagcagcg caaatagagt gcggcggagt gtggagagca tggtaacccc
    12601 aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat
    12661 tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc
    12721 cattgcacac tgtcggcggt gagcgtaaag ggggtggggg tgcagatcgc aaggcttaaa
    12781 ttttctagct gacaatggct gactgacagc gaggagtgat aggtgctatc tacgctgacg
    12841 atttgccagg cgctgccgcc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc
    12901 cagaacattt ccagttgctg atcaggatgc acggcgtcca tttcagcgtg ttccccgagg
    12961 ctgaacacgg cgttcgccag ccagcgccag ttgacgcgcg ggcgaatcac ttcgatatcg
    13021 cagccgactt cgccttcatc gctcaacagc agagcgatat cgtcaccgct atggcttaag
    13081 ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc cttgttcgcc atagatgatc
    13141 tccggtagcg gggaaagcgt gtgcgaaagc aatgtacgcc ccgccagcca gcgttcgcgt
    13201 cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc
    13261 gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatgg aaaatgaaag
    13321 tgcgtatcgt atcacttgtc gcctcatccc ggtaaccgac ttttcggtct gcccggcccc
    13381 agtaaaatcg ccagtttgct gccacctttc gtcgtttcca tccagatttt acacacgctg
    13441 gttaatggca ccgaaagtag catccctacc gggccgagca gccatcccca gattaacaat
    13501 gaaagaaata ccaccatggt ggacatcccc aggcgatggc ccatcatccg tggttctaaa
    13561 atattgccga tgaccatatg gaccactaaa aacaatgcgc cgaccagaat acattcgtaa
    13621 acaccattaa acagcagcac ctgaatcatt ggcggtacgg cggaaattac cgcgccgata
    13681 ttgggcacgt agttgagcaa aaacgccagt actgcccaca tcagcgcaaa ctgtacgccc
    13741 attagcgcca gccccagcca gacgatgaca cctgtccata aactgagtag cgtctttaac
    13801 gccagataat gcgaaacgcc tttaagtgcg cggtgtaatc ctgcgatgtg aatctgtggg
    13861 ttattcagcg caaaacgcat tttgtaaggg acgtggcgca cttcaaacag cataaaaact
    13921 acggtcatca ccagcaaaag cacgctcgcc attgccccgg aaagcccggt cattagcgct
    13981 gtggtgaagg taaccacttt ttccgagtcc atccgctgca gcattcgctc cggcgacata
    14041 tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa gtttgcgcgt cagctcctta
    14101 ttaaacttcg gcagcataga gataaattcg ttaaacgatg ccgccagtac gccgaccagc
    14161 gcggttagtg cgatcagcat caccaccact acaatcgtaa tggcaacggg gcgttgtact
    14221 ccccgacgaa taaaccaggt aacgagcggg ttgaggacga tggcaaaaaa cagcgccagt
    14281 aacagctgca caatgatatc tgccgctgcg tgaatgcccg cgaggatcac taccagcgag
    14341 gccaacttga gcagaatgtg catgcccgtt ttatcgggtt gaggggtttc cattggggct
    14401 tccttgtgac tttttgtgtt aagtgtagcg cgggagtcac gccagcaata ttctgattct
    14461 tactgctgat tttcacgtca gcacatggta aaaatgaaac actgttgtaa aaatgtggtg
    14521 atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc
    14581 attgtctcca ttgtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct
    14641 gtattaccgg gctatgtcca tgatgtgatg ggctttagcg ccttctgggc aggattggtt
    14701 atcagcctgc aatatttcgc caccttgctg agccgccctc atgccggacg ttacgccgat
    14761 ttgctgggac ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg
    14821 gggtatctga cggcaggatt aaccgccagt ctgcctgtca tcagcctgtt attactttgc
    14881 ctggggcgcg tcatccttgg gattgggcaa agttttgccg gaacgggatc gaccctatgg
    14941 ggcgttggag tggttggctc gctgcatatc gggcgggtga tttcgtggaa cggcattgtc
    15001 acttacgggg cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc
    15061 ttgcaggcgt tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc
    15121 ccgcgtccga cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg
    15181 cgcgtctggc tgtacggtat ggcgctggca ctggcttccg ccggatttgg cgtcatcgcc
    15241 acctttatca cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg
    15301 ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taaccgtatc
    15361 ggtggcttaa acgtagcgat gatttgcttt agcgttgaga taatcggcct gctactggtt
    15421 ggcgtggcga ctatgccgtg gatggcgaaa atcggcgtct tactggcggg ggccgggttt
    15481 tcgctggtgt tcccggcatt gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag
    15541 ggggcggcgc tggcaactta caccgtattt atggatttat cgcttggcgt gactggacca
    15601 ctggctgggc tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga
    15661 ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc
    15721 cctgaggccg cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc
    15781 tgcggtggtt tgcgcttttt gctgatcgtc agctggcagc gtaatttgca tggtcagcag
    15841 ttgattaccc acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat
    15901 aatactgtct aactgctgca ttttgtgacc tttcagctca atggctttat tggttaccac
    15961 ttgcagctgc ggatcgcggc tacgttgctg atcttccaga cgcttcgcca acaccgccag
    16021 atcttctttc ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga
    16081 ccagacatgc atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc
    16141 tggcagcgag aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact
    16201 ttccgcaacc gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc
    16261 cataaccagc aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc
    16321 gataaacggc cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta
    16381 actcgcctgc aaagcgttga tttcggattt atctgccagt cttttcaaca gcatattgag
    16441 taatacgcca tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc
    16501 gaccagcgcg atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc
    16561 aatgaagaaa aaggccagcg tgtcgctgac gttaccgaac agtgtggacg ctgtcggtgc
    16621 cagccaccag cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga ggatttgccc
    16681 cagcgcgtag gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc
    16741 gagtgcgccg aatccctgcc aggaacccat atagaatagc gacgagatga cgtaggagat
    16801 taataacgca gggatcatta ccgcgaagat aatgcgtcgg gccagcggtg cgccaaaaat
    16861 acgcacggtc aggtcggtag caagaaaaat aaacggaaag ctaaacgcgc cccaggtggt
    16921 atggaaacca aaaatggaga cgggaagctg aaccagatag ttactggagg tgatcaccag
    16981 aagatgaaat aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt
    17041 catattgtac ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct
    17101 gttaccttaa cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta
    17161 cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca
    17221 cagggcgtaa actagcgccg tttttttaag tgatgagaag aaaatgaccg atctcttttc
    17281 cagccctgac cacacactcg acgcgcttgg cctgcgctgc ccggaaccgg tgatgatggt
    17341 gcgcaaaacc gtgcgcaata tgcagcctgg cgaaacgttg ctgattatcg ccgacgatcc
    17401 ggccactacc cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgcgaa
    17461 agagacggat ggactgcctt atcgttattt gattcgtaaa ggcggttgat aggggctgat
    17521 tggcttcgat gccgcctttt cctcaccccc taaccctctc cccagagggg cgaggggacc
    17581 gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaacc ttaacgcatt
    17641 cgccgtcacc agcaccgtcg cacccgtatc tgccagcact gccagccaca acccggtcat
    17701 ccctaaaagc gtggtgacga ggaagatccc tttcagtccc agcgcaatgg tgatgttctg
    17761 gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg
    17821 gttatgggtt aatgctgcgt cggcggtttc cagcgccacg tctgtgccgc tacccatagc
    17881 aatcccgatg gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac cgaccatcgc
    17941 caggggcgca tgttgattca gcttggtcac cgctttgact ttatcttccg gcaataggcc
    18001 cgctttaaac tccagcccca gctccccggc aattgccgcc gctgcgcgtg gattatcgcc
    18061 ggtgaggatc actcctttaa cccccagcgc gttcagttca ctgatggcag ttgtagcgtc
    18121 ggcgcgcagg gtatcctgca atgcaatgat acccagcacg tcatcattac gtactaccag
    18181 cacaaccgtt tgcccggcgc tttccagttc attaatcaaa ccagcaaatg catcagcggg
    18241 atgtttcccg gcagcgcata tcaacacgcg ctcaccatta acctgcgctt caatgccaga
    18301 cccgaccagt gcccgctgtg attcggcggt gggaatggcg agttcagcaa cctgtgcttc
    18361 gcgtacgatg gcttgcgcca gtggatgcgt cgcgccttgc tcgaccgccg ccgccagtgt
    18421 cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc
    18481 gacggtcagc gtaccggttt tatcaaacgc cacttgggta acacgaccta gttgttccag
    18541 cgccgcaccg cctttaatta acgccccacg acgcgctgct gctgccagcc cggaggttat
    18601 cgccgcaggc gttgagataa ctaacgcaca cgggcaacca atcagcagca gcgtcagacc
    18661 tttataaatc cactcctgcc agctggcggc aaacagcagc ggcggcacta gcgttaccag
    18721 cagggcgacg gccataatcg ctggcgtata aatacggctg aaacggtcga taaaccgctc
    18781 aatgggggcg cggcgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct
    18841 ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg tggctcctgc
    18901 aggaacttta tcgcccgtcg cgcgctccac cggaatggat tctccggtta gggcgctttc
    18961 atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgcc cacctgcggc
    19021 gacttcaatc acatcgccag gccgcaggct gttaatcgcc acctcttccc gctcaccgtt
    19081 acgcaggcgc gtggcggttt ctggtttcag cgccattaac gcgctaacgc cctgacgcgc
    19141 gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca acaccatcgc
    19201 agcttcagcc gttgcgccaa taaacagtgc accaatagcg gctacgctca ttaaggtttc
    19261 aatggcgaag tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa tcgggtacag
    19321 cccaactagc gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag
    19381 accccagctg attgccatca tcacgataag cgtaatcagc ggcagattct ctttcaggcg
    19441 cgatgcttgt ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg ctttttgcac
    19501 cgcagattca acctgggcac ggatgtcatt gtcggcatca accaccagtt tttcggtggc
    19561 gaacaacacc tgcacctgat tcacgcctgc aagctggcgc acggcatttt ctaccttgcg
    19621 cgcacaggcg gcgcagtcca tgccgctgac tttccagcta tagcgggtgc cggagacgtt
    19681 ttcagagagc gttggcgagc tggagcatgc gccgtcgcag caacagtcgt tggcgttctg
    19741 taccgtggtt agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg
    19801 tgtcgacatg gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag
    19861 tcgactccag agtcaagttt tatcagagat acagtgagcg gacgatgagg aagtgaccgg
    19921 caaagtagca ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtggctcc
    19981 ccagccagac aaagttactg ataaacagca acgaagcgcc aacaaacgca gagagcgccg
    20041 gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc
    20101 cgataaacgt gcagataggc caacggtact cttccagccg cgtccagata atcgccagta
    20161 gcagcgcacc cagaaccagc aacaccagtg gcagaggcca gaagaaagag agcgtcatct
    20221 gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca
    20281 tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagtcccg
    20341 ccagcaccag atagctaatg gcgtcgaaca tcggtgcttg ccaggccagt aacagcagga
    20401 gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga tacgatgcat
    20461 ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt
    20521 gaattcgtta ttttttcgta agcagaatgc ctacggtctt gcttccagtt tagtggcgag
    20581 ggtggacgga tgacaacacc ataactggca gggtatgctt aattccgcat tttccgatga
    20641 gggataaaga tgagtaaacc acctcttttc tttattgtta tcattggctt aattgtcgtc
    20701 gccgcatcgt ttcgttttat gcaacagcga cgggaaaaag ctgataatga tatggctccg
    20761 ctccagcaaa agctggtggt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt
    20821 tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctatgaggc aagcttcaaa
    20881 ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg cccagcagta ccacgccctg
    20941 acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgttag ctttgttggc
    21001 gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg
    21061 ccgaaaagga aaatccgcac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa
    21121 gtggatttca atagcgccag ctgcatacca tgcatcagca cggtaaaaat cagcgccacg
    21181 ttaacgaaaa tatttagcgg gcgcgggaag ggatgcacca gattgaggat taaaaatccc
    21241 caaacgcaga gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc
    21301 gtgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt
    21361 tgccggaaca gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc
    21421 agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa
    21481 cggtggatcg acaaacacga tattatgcgg tgtgcctttt tgcgccagga atgacattgc
    21541 gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg
    21601 ctgagaaacc acgcgatcca tctcaatcaa cgttgccccc gcagcgtagc gcgataacgc
    21661 ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt caacaatgac
    21721 cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg
    21781 gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc
    21841 gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac
    21901 ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc
    21961 tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg
    22021 cgaaagaaaa aagacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc
    22081 cggaaaaaga gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc
    22141 aagagcctgc gaaggcttct gaacaagccg ttgaagagca gccgcaggcg catactgaag
    22201 ccgaggcgga aacttttgct gccgacgttg tggaagtcac tgaacaggtt gttgaaagtg
    22261 aaaaagcgca gcctgaagcg gaagttgttg cacagccgga accggtcgta gaagaaacgc
    22321 cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc ggaagacgtc aacgccgaag
    22381 aggtttcgtc agaagagtgg caggctgaag cggaaaccgt agagattgtc gaagcggcag
    22441 aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg
    22501 ccgaagcggc agaagaggcg gtgatggtag ttcctccggc agaagaagag cagccggttg
    22561 cagaaattgc ccaggagcaa gaaaaaccga ccaaagaagg cttcttcgcg cgcctgaaac
    22621 gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta
    22681 aaaaaatcga cgatgatctg tttgaagagc tggaagaaca gctgttgatc gccgatgtgg
    22741 gcgtggaaac cacacgtaaa attatcacca atctgacgga aggcgcatcc cgcaagcagc
    22801 ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggcgag attctggcga
    22861 aagtcgatga gccgctgaat gttgaagata aaacgccgtt cgtgatcctg atggtgggcg
    22921 tcaacggtgt gggtaaaacc acgacgattg gtaagctggc gcgtcagttt gagcagcagg
    22981 gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc agctgcggtt gaacagcttc
    23041 aggtctgggg tcagcgcaac aatattccgg tgattgccca gcataccggt gcggattccg
    23101 cctctgttat cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg
    23161 ccgataccgc cggacgtctg cagaacaaat cgcatctgat ggaagagttg aagaaaatcg
    23221 tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg actattgatg
    23281 ccagcaccgg gcagaacgcg gtaagccagg ccaaactgtt ccacgaagct gttggcttaa
    23341 ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt ttctcggtgg
    23401 ctgaccagtt tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc
    23461 gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat
    23521 tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac
    23581 gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag
    23641 taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag
    23701 cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg
    23761 catgattttc caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat
    23821 cccgctgatt atcgctggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcact
    23881 ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt ccggcggtga
    23941 acaacagcgt gttggcattg cccgcgcggt ggtgaataag cccgcggtac tgctggcgga
    24001 cgaaccgacc ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc tgtttgaaga
    24061 gtttaaccgc gttggggtaa ccgtattgat ggcaacgcac gacattaatc tgatttcacg
    24121 gcgttcctat cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga
    24181 ataagcgcga tgcaatcaat catattcggc agtttggtgg gcgtctggat cgcttccgta
    24241 aatcggtcgg cggctcaggc gacggcggtc gtaacgcacc gaaacgcgcg aaatcctcgc
    24301 caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg
    24361 gcgcattgca ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg
    24421 ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aatcaggcgg
    24481 cgacgcagta ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg ctggacgatg
    24541 acgccgctgc gggcgtggtg gcacagttgc aggccgagca aggcgtggag aaagtgaact
    24601 atctttctcg tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc
    24661 tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt
    24721 ttcaggggac ggaatcactc aatacgctgc gtgatcgtat cacgcagatt aacggtattg
    24781 acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttgacc gggctggttg
    24841 ggcgcgtttc ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc gtcatcggca
    24901 acagcgtacg tctgagcatc tttgctcgcc gtgactccat caacgtgcag aaactgattg
    24961 gtgcgacgga tggtttcatc cttcgcccgt tcctgtacgg cggtgcgctg ctgggatttt
    25021 ctggcgcatt gttgtcatta attttgtcag aaattctggt actgcgtttg tcgtcagcgg
    25081 tggcggaagt ggcacaggtt ttcggaacga agtttgatat caatggttta tcgttcgatg
    25141 aatgcctgct attgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca
    25201 cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata ctctttccct
    25261 gcaatgggtt ccgtagcagg gaaagagacc ccgttgtctc ttcccggtat ttcatctcta
    25321 tgtcacattt tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcacg
    25381 gtctgataaa acagtgaatg ataacctcgt tgctcttaag ctttggcaca gttgttgcta
    25441 ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt
    25501 ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg
    25561 atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg
    25621 gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat
    25681 tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg
    25741 aaagcagtgc gccgttttaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgtccac
    25801 tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg
    25861 accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaaccaagca gcgtctgggc
    25921 tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac
    25981 gtacgtgaga tggaatcacg tatggcggca caggacatga cctttgacct gtcttccgac
    26041 gacgattccg acagccagcc gatggcaccg gtgctctatc tgcaggataa atcatctaac
    26101 tttgccgacg gcatcgaaga tgataactgg gaagagcagg cggcaaaccg tctgaccgac
    26161 gcgatgcatg gtctggacga acgcagccag gacatcatcc gtgcgcgctg gctggacgaa
    26221 gacaacaagt ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgaacgtgtg
    26281 cgccaactgg aaaagaacgc gatgaaaaaa cttcgcgccg ctattgaagc gtaatttccg
    26341 ctattaagca gagaaccctg gatgagagtc cggggttttt gttttttggg cctctacaat
    26401 aatcaattcc ccctccggca aaacgccaat ccccacgcag attgttaata aactgtcaaa
    26461 atagctattc caatatcata aaaatcgggt atgttttagc agagtatgct gctaaagcac
    26521 gggtagtcat gcataaaacg aaataaagtg ctgaacaaca acatcacaac acacgtaata
    26581 accagaagaa tggggattct caggatgaac ataaagggta aagcgttact ggcaggatgt
    26641 atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc ggtcgtgggt
    26701 gcaatgtccg gtccggttgc gcagtacggt gaccaggagt ttaccggcgc agagcaggcg
    26761 gttgcggata tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat cgtaaaatat
    26821 gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt taacgacggc
    26881 attaaatatg tgattggtca cctctgttct tcatcaacgc agcctgcgtc tgacatctac
    26941 gaagacgaag gcattttaat gatcacccca gcggcaaccg cgccggagct gaccgcccgt
    27001 ggctatcagc tgatcctgcg caccaccggc ctggactccg accaggggcc gacggcggcg
    27061 aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga caaacagcaa
    27121 tacggcgaag gtctggcacg agcggtgcag gacggcctga agaaaggcaa tgctaacgtg
    27181 gtgttctttg atggtatcac cgccggggaa aaagatttct caacgctggt ggcgcgtctg
    27241 aaaaaagaga atatcgactt cgtttactac ggcggttatc acccggaaat ggggcaaatc
    27301 ctgcgtcagg cacgcgcggc agggctgaaa actcagttta tggggccgga aggtgtggct
    27361 aacgtttcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg
    27421 aagaactacg atcaggttcc ggcgaacaaa cccattgttg atgcgatcaa agcgaaaaaa
    27481 caggacccaa gcggcgcatt cgtctggacc acctacgccg cgctgcaatc tttgcaggcg
    27541 ggcctgaatc agtctgacga tccggctgaa atcgccaaat acctaaaagc gaactccgtg
    27601 gataccgtga tggggccact gacctgggat gagaaaggcg atctaaaggg ctttgagttc
    27661 ggcgtatttg actggcacgc caacggcacg gcgaccgatg cgaagtaatt gttaatcggc
    27721 aactttgggt tgccgccaaa ttgctaatat cgaatacgtt gcttcatgcc ggatgcggcg
    27781 taaacgcctt atccggccta caagaaccaa agaaatcagt aaattgcagc acacattata
    27841 ggcctgataa gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact
    27901 tgacgattaa cgcttctccc agccgccctg ctgtgccgta aatcccagtg cctgcataaa
    27961 cgccgtcatc acaccgcgat cttccacgcc tgcatccgcc atccaccagc atgaaacgcc
    28021 aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac ggcgggtgac
    28081 ttcccgcacg cgtagagaat ccagtgctcc ctcggtgccg cttaaggtta cccgcacggc
    28141 cgcaagcaga cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt caacctgtaa
    28201 agaggaaggg gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa
    28261 gttttctaat cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac aaaagtggaa
    28321 ccagtgtagc gaaataattt aatcggaggc tttctctttt ttatttcttt tggcaggtga
    28381 ttaatttttt aacagcaata attacaaagt taaaacatta gagaatgaaa aatgtccagc
    28441 ataatcccct gaatgatagt gaattattcc gccctttgtg ccgt
//
LOCUS       DAZPDE010000035        28191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPDE010000035 DAZPDE010000000
VERSION     DAZPDE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.035"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHU0J_001421"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHU0J_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1216174.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHU0J_001422"
     CDS             complement(2138..2584)
                     /locus_tag="ACHU0J_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1216175.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHU0J_001423"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHU0J_001423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1216176.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHU0J_001424"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHU0J_001424"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1216177.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHU0J_001425"
     CDS             complement(5347..6009)
                     /locus_tag="ACHU0J_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1216178.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHU0J_001426"
     CDS             6182..6595
                     /locus_tag="ACHU0J_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1216179.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHU0J_001427"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHU0J_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1216180.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHU0J_001428"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHU0J_001428"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR1216181.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHU0J_001429"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHU0J_001429"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1216182.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHU0J_001430"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHU0J_001430"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1216183.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHU0J_001431"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHU0J_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1216184.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHU0J_001432"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHU0J_001432"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHU0J_001433"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHU0J_001433"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1216185.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHU0J_001434"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHU0J_001434"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1216186.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHU0J_001435"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHU0J_001435"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR1216187.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHU0J_001436"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHU0J_001436"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1216188.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHU0J_001437"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHU0J_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1216189.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHU0J_001438"
     CDS             14984..15841
                     /locus_tag="ACHU0J_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1216190.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHU0J_001439"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHU0J_001439"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1216191.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHU0J_001440"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHU0J_001440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1216192.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHU0J_001441"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHU0J_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1216193.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHU0J_001442"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHU0J_001442"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1216194.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHU0J_001443"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHU0J_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHU0J_001444"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHU0J_001444"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1216195.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHU0J_001445"
     CDS             complement(22884..24023)
                     /locus_tag="ACHU0J_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1216196.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHU0J_001446"
     CDS             complement(24069..26165)
                     /locus_tag="ACHU0J_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1216197.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHU0J_001447"
     CDS             complement(26165..26911)
                     /locus_tag="ACHU0J_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1216198.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHU0J_001448"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHU0J_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1216199.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHU0J_001449"
     CDS             complement(27659..27964)
                     /locus_tag="ACHU0J_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216200.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         6779 a         7188 c         7452 g         6772 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa g
//
LOCUS       DAZPDE010000036        28078 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPDE010000036 DAZPDE010000000
VERSION     DAZPDE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.036"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHU0J_001450"
     CDS             complement(<1..209)
                     /locus_tag="ACHU0J_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1216201.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHU0J_001451"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHU0J_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1216202.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHU0J_001452"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHU0J_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1216203.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHU0J_001453"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHU0J_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1216204.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHU0J_001454"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHU0J_001454"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1216205.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHU0J_001455"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHU0J_001455"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1216206.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHU0J_001456"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHU0J_001456"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1216207.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHU0J_001457"
     CDS             complement(4598..5320)
                     /locus_tag="ACHU0J_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1216208.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHU0J_001458"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHU0J_001458"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1216209.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHU0J_001459"
     CDS             6520..6738
                     /locus_tag="ACHU0J_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1216210.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHU0J_001460"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHU0J_001460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1216211.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHU0J_001461"
     CDS             complement(7472..7672)
                     /locus_tag="ACHU0J_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1216212.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHU0J_001462"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHU0J_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHU0J_001463"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHU0J_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1216213.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHU0J_001464"
     CDS             10101..12014
                     /locus_tag="ACHU0J_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1216214.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHU0J_001465"
     CDS             12014..13036
                     /locus_tag="ACHU0J_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1216215.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHU0J_001466"
     CDS             13030..13248
                     /locus_tag="ACHU0J_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1216216.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHU0J_001467"
     CDS             13302..14171
                     /locus_tag="ACHU0J_001467"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1216217.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHU0J_001468"
     CDS             complement(14226..14630)
                     /locus_tag="ACHU0J_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1216218.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHU0J_001469"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHU0J_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1216219.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHU0J_001470"
     CDS             15615..17705
                     /locus_tag="ACHU0J_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1216220.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHU0J_001471"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHU0J_001471"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1216221.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHU0J_001472"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHU0J_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1216222.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHU0J_001473"
     CDS             complement(19673..20275)
                     /locus_tag="ACHU0J_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1216223.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHU0J_001474"
     CDS             complement(20265..20432)
                     /locus_tag="ACHU0J_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1216224.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHU0J_001475"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHU0J_001475"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1216225.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHU0J_001476"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHU0J_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHU0J_001477"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHU0J_001477"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1216226.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHU0J_001478"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHU0J_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1216227.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHU0J_001479"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHU0J_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1216228.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHU0J_001480"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHU0J_001480"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1216229.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6805 a         7513 c         7087 g         6673 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtaa cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagca
//
LOCUS       DAZPDE010000037        27601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPDE010000037 DAZPDE010000000
VERSION     DAZPDE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.037"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHU0J_001481"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHU0J_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1216230.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHU0J_001482"
     CDS             complement(1614..1937)
                     /locus_tag="ACHU0J_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1216231.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHU0J_001483"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHU0J_001483"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1216232.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHU0J_001484"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHU0J_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1216233.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHU0J_001485"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHU0J_001485"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1216234.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHU0J_001486"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHU0J_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1216235.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHU0J_001487"
     CDS             7537..8574
                     /locus_tag="ACHU0J_001487"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1216236.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHU0J_001488"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHU0J_001488"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1216237.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHU0J_001489"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHU0J_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1216238.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHU0J_001490"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHU0J_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1216239.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHU0J_001491"
     CDS             10745..11626
                     /locus_tag="ACHU0J_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1216240.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHU0J_001492"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHU0J_001492"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1216241.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHU0J_001493"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHU0J_001493"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1216242.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHU0J_001494"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHU0J_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHU0J_001495"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHU0J_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1216243.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHU0J_001496"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHU0J_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1216244.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHU0J_001497"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHU0J_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1216245.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHU0J_001498"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHU0J_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1216246.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHU0J_001499"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHU0J_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1216247.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHU0J_001500"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHU0J_001500"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1216248.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHU0J_001501"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHU0J_001501"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1216249.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHU0J_001502"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHU0J_001502"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1216250.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHU0J_001503"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHU0J_001503"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1216251.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHU0J_001504"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHU0J_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1216252.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHU0J_001505"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHU0J_001505"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1216253.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHU0J_001506"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHU0J_001506"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1216254.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHU0J_001507"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHU0J_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1216255.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHU0J_001508"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHU0J_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1216256.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHU0J_001509"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHU0J_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6806 a         6866 c         7237 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZPDE010000038        27373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPDE010000038 DAZPDE010000000
VERSION     DAZPDE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.038"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..576
                     /locus_tag="ACHU0J_001510"
     CDS             <1..576
                     /locus_tag="ACHU0J_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1216257.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACHU0J_001511"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACHU0J_001511"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR1216258.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHU0J_001512"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACHU0J_001512"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1216259.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHU0J_001513"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACHU0J_001513"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1216260.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHU0J_001514"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACHU0J_001514"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1216261.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHU0J_001515"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACHU0J_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1216262.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACHU0J_001516"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACHU0J_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1216263.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACHU0J_001517"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACHU0J_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1216264.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACHU0J_001518"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACHU0J_001518"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1216265.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACHU0J_001519"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACHU0J_001519"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1216266.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACHU0J_001520"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACHU0J_001520"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1216267.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACHU0J_001521"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACHU0J_001521"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1216268.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHU0J_001522"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACHU0J_001522"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1216269.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHU0J_001523"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACHU0J_001523"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1216270.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACHU0J_001524"
     CDS             13936..14316
                     /locus_tag="ACHU0J_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1216271.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACHU0J_001525"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACHU0J_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1216272.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHU0J_001526"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACHU0J_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1216273.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHU0J_001527"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACHU0J_001527"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1216274.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACHU0J_001528"
     tRNA            complement(17417..17492)
                     /locus_tag="ACHU0J_001528"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACHU0J_001529"
     tRNA            complement(17496..17571)
                     /locus_tag="ACHU0J_001529"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACHU0J_001530"
     tRNA            complement(17623..17698)
                     /locus_tag="ACHU0J_001530"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACHU0J_001531"
     tRNA            complement(17701..17776)
                     /locus_tag="ACHU0J_001531"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACHU0J_001532"
     tRNA            complement(17912..17987)
                     /locus_tag="ACHU0J_001532"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHU0J_001533"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACHU0J_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1216275.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHU0J_001534"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACHU0J_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1216276.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHU0J_001535"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACHU0J_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1216277.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHU0J_001536"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACHU0J_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1216278.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEIAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHU0J_001537"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACHU0J_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1216279.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHU0J_001538"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACHU0J_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1216280.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHU0J_001539"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACHU0J_001539"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1216281.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHU0J_001540"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACHU0J_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1216282.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHU0J_001541"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACHU0J_001541"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1216283.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHU0J_001542"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACHU0J_001542"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1216284.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHU0J_001543"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACHU0J_001543"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1216285.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6889 a         7255 c         6704 g         6525 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaatgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctatct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaaa gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgc
//
LOCUS       DAZPDE010000039        27124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPDE010000039 DAZPDE010000000
VERSION     DAZPDE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.039"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="ACHU0J_001544"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="ACHU0J_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR1216286.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="ACHU0J_001545"
     CDS             712..819
                     /locus_tag="ACHU0J_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1216287.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1195..1302
                     /locus_tag="ACHU0J_001546"
     CDS             1195..1302
                     /locus_tag="ACHU0J_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1216288.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(1389..3068)
                     /gene="bcsG"
                     /locus_tag="ACHU0J_001547"
     CDS             complement(1389..3068)
                     /gene="bcsG"
                     /locus_tag="ACHU0J_001547"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1216289.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3065..3256)
                     /gene="bcsF"
                     /locus_tag="ACHU0J_001548"
     CDS             complement(3065..3256)
                     /gene="bcsF"
                     /locus_tag="ACHU0J_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1216290.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(3253..4824)
                     /gene="bcsE"
                     /locus_tag="ACHU0J_001549"
     CDS             complement(3253..4824)
                     /gene="bcsE"
                     /locus_tag="ACHU0J_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1216291.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            5097..5285
                     /gene="bcsR"
                     /locus_tag="ACHU0J_001550"
     CDS             5097..5285
                     /gene="bcsR"
                     /locus_tag="ACHU0J_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1216292.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            5297..6049
                     /gene="bcsQ"
                     /locus_tag="ACHU0J_001551"
     CDS             5297..6049
                     /gene="bcsQ"
                     /locus_tag="ACHU0J_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1216293.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            6046..8663
                     /gene="bcsA"
                     /locus_tag="ACHU0J_001552"
                     /pseudo
     CDS             6046..8663
                     /gene="bcsA"
                     /locus_tag="ACHU0J_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8674..11013
                     /gene="bcsB"
                     /locus_tag="ACHU0J_001553"
     CDS             8674..11013
                     /gene="bcsB"
                     /locus_tag="ACHU0J_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1216294.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            11020..12126
                     /gene="bcsZ"
                     /locus_tag="ACHU0J_001554"
     CDS             11020..12126
                     /gene="bcsZ"
                     /locus_tag="ACHU0J_001554"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1216295.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            12321..12710
                     /locus_tag="ACHU0J_001555"
     CDS             12321..12710
                     /locus_tag="ACHU0J_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR1216296.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12792..14780
                     /gene="hmsP"
                     /locus_tag="ACHU0J_001556"
     CDS             12792..14780
                     /gene="hmsP"
                     /locus_tag="ACHU0J_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1216297.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14970..16256
                     /gene="dctA"
                     /locus_tag="ACHU0J_001557"
     CDS             14970..16256
                     /gene="dctA"
                     /locus_tag="ACHU0J_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1216298.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            16477..17973
                     /locus_tag="ACHU0J_001558"
     CDS             16477..17973
                     /locus_tag="ACHU0J_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1216299.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18069..18998)
                     /gene="kdgK"
                     /locus_tag="ACHU0J_001559"
     CDS             complement(18069..18998)
                     /gene="kdgK"
                     /locus_tag="ACHU0J_001559"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1216300.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19230..19997
                     /gene="pdeH"
                     /locus_tag="ACHU0J_001560"
     CDS             19230..19997
                     /gene="pdeH"
                     /locus_tag="ACHU0J_001560"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1216301.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20067..22127
                     /locus_tag="ACHU0J_001561"
     CDS             20067..22127
                     /locus_tag="ACHU0J_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1216302.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22309..23631)
                     /locus_tag="ACHU0J_001562"
     CDS             complement(22309..23631)
                     /locus_tag="ACHU0J_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216303.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24042..25055)
                     /gene="yhjD"
                     /locus_tag="ACHU0J_001563"
     CDS             complement(24042..25055)
                     /gene="yhjD"
                     /locus_tag="ACHU0J_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1216304.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25104..26003)
                     /locus_tag="ACHU0J_001564"
     CDS             complement(25104..26003)
                     /locus_tag="ACHU0J_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1216305.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26523..>27124
                     /locus_tag="ACHU0J_001565"
     CDS             26523..>27124
                     /locus_tag="ACHU0J_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1216306.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6488 a         6799 c         7413 g         6424 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcgtcaagc cggaagggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggccatt cttgaacctg
      661 aacaactcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaacaag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggaccag gccgcagggg tgaaactctg cggccttttt
      901 cgtaagaaaa atgcaacgat tgcacgcgga taatattctg cggaatgcgc agcacagagt
      961 gaagacgtga cggattccgg aacagaaaaa cagtcgtaga atcagcggca agccggaaag
     1021 gttccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt tgctggagag
     1081 agaaaacccc cgcacgttgc aggtatgcac ctgacaacat cacgggggct aatcttgaac
     1141 ccaaacaact caagaatagc cgcgaaacgt tggcattaca acacaggcgg ctatatgacg
     1201 ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggcatt
     1261 cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat gcgggcgtca
     1321 ggctgccgta atggcaattt gcgcccggat caggccgcag aggttaaact ctgcggcctt
     1381 tttcgtgctt actgcgggta aggcacccag tcgccgccgt tcaggcgaac gtacggttta
     1441 tcttggtatt gaataactac tgcatttgag ttctcggaga ccggtgctgt ttgtggcaac
     1501 ccactggtga gttttttcca gtcaacattg tcttcggtga aaatcttgcc atcaagaacg
     1561 cgaaccacca gatcggagat agccaggaag ctgctcggtt ggtcaatgac aatcggtgcc
     1621 ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa cggggacgtc tgtgatagac
     1681 gggctaggga tatcacgtag gccagatacc tgcattctgt cgcccttcag cgcgccgccg
     1741 tgttccggca ccacgaccac catcacttta cgacccgatt tctccagttc agtaaagaag
     1801 gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt aatccgctgt tttgctgacc
     1861 cccggataat ggttgccgtc atgcagtgga agcgtgttgt agaacgtggc actacggctg
     1921 tttttatctt tttcggtaac gtccagccag cggttaagca cggcggtatc gtcataaacc
     1981 ggcgaaccat caaagcccag caaaataacc ggcagatttg tttgatccat caattcagtc
     2041 tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc cgaactggcc gttatgtccc
     2101 atcatcaggt gctgggtaaa gcccagtttc gaaaggttat caaacagata gcagtcgtta
     2161 tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc agctggcgcg cagtaaacgg
     2221 atcgccgccg ggccactgta ggaggtggcg gagttaaagt tcttgaaatc aatatcgaaa
     2281 tgcgaccaca gtggatgcga catcaacccg gcggcttcta tatccgacca ggaaagcgaa
     2341 cagatgttaa tcaccagtag ttcaaatggc tgagcatcag cgggcagcga agacgggaag
     2401 gtcgatttac gtttcgcctc cgcgttatag aaattattca gccaggcgtt aaggttcgcc
     2461 gttgttggcg gtgcagtttg tgcgggcata tcacccacta ccggtgcgcc acccgtcgcc
     2521 gcaacggttg ccgctgcgtt accacccgtc gttgttacag tggtcgtcgg ttgtccggct
     2581 ggccacaagg agaaacttgg tcccgccagg gtaagtacgt tcagccatag cagtatggca
     2641 accacaaaaa cggtaatgcg aatccattgt gacaggaata accaggccac taataaaaca
     2701 aaaatggccc caatcatctg ccagttaata aagcgtgtga caaggtcgat taaataatcg
     2761 gtactgaacc ccgccacctg cgaaccctgg ctcattatgc tttccgggcc aggcaaccag
     2821 atgtcatgcc agaacaaagc aaagccgatc ggcagggcaa tccagtggcg caagcgatgc
     2881 aggctgtaac gcggaatggg catcagcaga aacgcggcaa acaccaaatt gaggagcggg
     2941 tggaagttaa gatatcccgc ccacaacagg ccaaacttaa ccagaaaata gaagttccag
     3001 ccggaaaggc cgcgccagta ttgccagagg gaagaaggca tggcggtatt ttgcgtaaat
     3061 tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg taacgtcccg gccggtttaa
     3121 cataacgagg tttagcaaag aacaaacgta aggtgtcgcg aatgcgtcgc aaagagtgcc
     3181 gcgccagata gcccagcggg aaaaatatca gtgcgcaaac gataataatt tcaatgatat
     3241 cgctgatggt catcatgatg agcgctccac agcatcatct aacagtcgca tgggttctgg
     3301 tattcgtcgc cagtggcgac catcgtgctc ggcattgatg accggtttag aactttgcgt
     3361 taaaggcagc ggcattcccc attgttctgg ggcaagcagg cgcatctgca ccagctcggc
     3421 actgatttga tcatcttcaa accagaccat acggtttgag aaaatgtcgc cagtaggcaa
     3481 tgggaaaata tgattcaacg cggtatccag atcgttaatc cgacagaatg agagaaacag
     3541 caccagccga ttaccgccaa tggtcatgat atcgccggta cggttagggc gacatagcgt
     3601 cagggcttgc tcaacgcgga tacccggtac cggacgtagg gcaaccagaa cgcctttacc
     3661 gtgggcaggc aagagagggt tattcatcat gttgttgacg gcattacaga acacatccca
     3721 cttctggaaa ccacgcagtt tgagcggctg ggtcattgac agcaaggtag tgatatcttc
     3781 cggtacatag cgactaaact tctgcccttg cacgctttcg atcatcgtca gacaacggga
     3841 gagtggcgca ttccacggaa taaccatatt tgcaccgcag gccaataaca aacgttcatc
     3901 ggtggcgcgc aggctagcgg tattttcccg cacgaggatt ttcatcgcac taccgcgctg
     3961 gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt tgcgcatttt gctgtaaaga
     4021 aaagaccacc gtcgccgcct gagcggtacg ggcttcattg aacaggactt cgttattgtt
     4081 gaacagttgc cagtgttccg atagcggcgg cgcaccttcc agtacagcaa cattactcag
     4141 aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct tcgctttgaa ctaatgtcca
     4201 gataccattt tgttgctgaa cgctaagctg ctgacgggcg ctgacccctt tttcgttgca
     4261 ccagaaggca atatccagca aatgttggtc accctgaaaa cgcaaactgg caagaccaaa
     4321 aagtgaacgg tattcctcaa gcaacaatga aaattgttta tcgttattat ttccgggatt
     4381 aattaccaaa agcgaacaat ggtttaacct gctccattta ttcattttat ccaaccatga
     4441 gcgaagccgc tcggcaggaa tattttgcca tgcgttattt gcgcaaacaa gaataaaaag
     4501 gtaattatgg ggatcaatag aacactgcaa atcacgggcc aaatagtata gacctttttc
     4561 atgatttagc attgaaaata attttatttt ttccggccct tgagaatcat ctaattgaaa
     4621 gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg tgtgcggttt cagcctggga
     4681 tgcaattgtt tgattcgcca gactgatagc atcttcatgg cgatcgacgt taaaccacca
     4741 gacgccgcct gctggcatat gtcgcagctc atcccataat gatgagatac cgatagagaa
     4801 tacagggtcc acaatgtccc tcatgtcacc attttgtctg attatcagtt tactagcgaa
     4861 agctaagaaa taaacctacc attgaaatta aaacttatca gatttagcat gtgaatcaat
     4921 ttagaaggtc acaagacgtg tgattttagt cttttcgttt ttaacttttt ctgttgcgta
     4981 acggaaagtc aaaaagtgag caaattcccg tctcgccgta aacaataaac cggcgaaatg
     5041 ctcacggtta gaattgtctc atgaatggta cggttatttc atagggatca agcaaaatga
     5101 ataacaatga accagatact ctgcctgatc ccgcgatagg ctatatcttc cagaatgata
     5161 ttgtggcgtt aaagcaggca ttttcactgc ctgatattga ttatgccgat atttcccaac
     5221 gcgaacagtt ggccgcggca ttaaaacgct ggccgttgct ggcagagttt gcgcaacaaa
     5281 agtaggggat tggtgaatgg ccgtactggg attgcagggg gtgcggggag gcgtggggac
     5341 aacaaccatc accgccgcat tagcctggtc attacaaatg ttgggagaaa atgtcctggt
     5401 ggtcgatgcc tgcccggaca acttgttgcg cctgtcattt aatgttgatt ttacccaccg
     5461 tcagggctgg gccagagcga tgctggatgg ccaggactgg cgtgacgctg ggttgcgcta
     5521 cacctcgcag ctcgatttgc tgccttttgg tcagttatcc attgaagaac aagaaaatcc
     5581 acagcactgg caaacccggc tgagcgatat ttgctccggc ttacagcaac taaaagccag
     5641 cgggcgttac cagtggattt taatcgactt accgcgtgat gcctcgcaga taacccacca
     5701 gctgctgagt ttgtgcgagc actcgctggc aatcgtcaat gtggatgcca actgccatat
     5761 ccgactgcat cagcaagcgc tgccggatgg cgcacatatt ttgattaatg acttccgtat
     5821 tggcagtcag gttcaggacg atatttacca gctttggttg caaagccagc gccgattact
     5881 gccgatgctc attcatcgtg atgaagcgat ggctgaatgc ctggcggcta agcaaccagt
     5941 aggtgaatat cgcagtgatg cgctggcggc tgaagagata ctgacgctgg cgaactggtg
     6001 cctgttgaac tactccgggc tgaaaacgcc agtcgggagt gcatcatgag tatcctgacc
     6061 cggtggttgc ttatcccgcc ggtcaacgcg cggcttatcg ggcgttatcg cgattatcgt
     6121 cgtcacggtg cgtcggcttt cagcgcgacg ctcggctgtt tctggatgat cctggcctgg
     6181 atttttattc cgctggagca cccgcgctgg cagcgtattc gcgcagaaca taaaaacctg
     6241 tatacgcata tcaacgcctc gcgtccgcgt ccgctggacc cggtccgtta tctcattcaa
     6301 acatgctggt tattgatcgg tgcatcgcgc aaagaaacgc cgaaaccgcg cagcgggcat
     6361 tttcaggtct gcaaaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc
     6421 gcgttagcca taaaacacag catctggatg aaaaaaaaga gctcggtcat ttgagtgccg
     6481 gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct
     6541 gcgttactca gccgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtgggggg
     6601 tagcgctgat cgtacggcgg atgccggggc gcttctcggc gctaatgttg attgtgctgt
     6661 cgctgaccgt ttcttgccgt tatatctggt ggcgttacac ctctacgctg aactgggacg
     6721 atccggtcag cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg
     6781 tgctggtgct cggctacttc caggtagtat ggccgctgaa tcgtcagccg gtgccattgc
     6841 cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt cccgacttac aacgaagatc
     6901 tcaatgtggt gaaaaatacc atttacgcct cgctgggtat cgactggccg aaagacaaac
     6961 tgaacatctg gatcctcgat gacggcggca gggaagagtt tcgccagttt gcgcaaaacg
     7021 tgggggtgaa atatatcgcc cgcaccactc atgaacatgc gaaagcgggc aacatcaaca
     7081 atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt cgactgcgac cacgtaccaa
     7141 cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga
     7201 tgcagactcc acaccacttc ttctcgccgg acccgtttga acgcaacctg ggacgttttc
     7261 gtaaaacgcc gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt
     7321 gggacgccac tttcttctgc ggttcctgtg cggtgattcg tcgtaagccg ctggatgaaa
     7381 ttggcggcat tgctgtcgaa accgtgactg aagatgcgca tacttctctg cgtttgcacc
     7441 gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa
     7501 gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg cgggatggta taaatcttcc
     7561 gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc tcagcggctg tgctacgtca
     7621 acgccatgtt ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg
     7681 cgttcctgct gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctattcg
     7741 tgctgccgca tatgatccat gccagcctga ccaactcgaa gatccagggc aaatatcgcc
     7801 actctttctg gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc
     7861 tggtggcgct gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg
     7921 tggaagaaga gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctactca
     7981 acctggtggg cgttgcggta ggcatctggc gctacttcta tggcccgcca accgagatgc
     8041 tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct gattgttctt ggcggcgcag
     8101 ttgcggtatc ggtagaaagc aaacaggtac gccgatcgaa ccgcgtggag atgacgatgc
     8161 ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg
     8221 acggtggttt ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga
     8281 atctgttgct taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga
     8341 tgggtaatga agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg
     8401 tgcagtgtac gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag
     8461 ataagccgct ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc
     8521 tggcggagtt tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt
     8581 cctgggttgt atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg
     8641 atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt
     8701 gcagtggcta tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg
     8761 caaccactga tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg
     8821 gggcaagtga tgtcaggcga gcagggagct gatgccccca tcgtggcgca gaacggtcct
     8881 tcgcgtgatg tgaagctgac ctttgcgcaa atcgcgccgc cgccgggcag catggtgcta
     8941 cgtggtatta acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg
     9001 aaggcgatgc tcaacttcga atacacgcca tcgccatcgt tactgcctgt ccagtcgcag
     9061 ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc cagtgaccaa agaacagttg
     9121 ggtaaaaaaa cgctggcgca aatgcccatt aatccactgt ttattaccga cttcaaccgt
     9181 gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg
     9241 ctttggctgg atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg
     9301 aagaatgacc tgtcacactt cccggtgcca ttctttgacc cgcgcgataa ccgtactaac
     9361 accttgccga tggtctttgc gggtacgccg gatgttgggc tgcaacaagc atctgccatt
     9421 gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat
     9481 aaccaattgc cggatcgcaa tgccattgtc tttgcaacca acgacaaacg gccggacttc
     9541 ctgcgcgatc atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat
     9601 ccttacgtca aactgctggt ggtgtttggt cgtgacgaca aagacctgtt gcaggcagcg
     9661 aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa gcgtggtagt gaatgaagtg
     9721 aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact gggtacgtac cgatcgtccg
     9781 gtcacatttg gcgaactgaa aacctatgaa gaacagttac aatccagcgg ccttgagcca
     9841 gcagcgatta acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc
     9901 attgatatgg atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg
     9961 gatatcagcc tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg
    10021 aaccgtctgc tgctgcgtat tccggtatta caaggtttgc tggatggcaa aacagatgtc
    10081 tctattccgg cgctgaaact gggcgcgacc aaccagctgc gcttcgactt tgagtatatg
    10141 aacccgatgc caggcggttc ggtagataac tgtattacct tccagccggt gcagaatcat
    10201 gtggtgattg gtgacgattc caccatcgac ttctcgaagt attaccactt catcccgatg
    10261 ccggatctac gcgcctttgc taacgcgggc ttcccattca gccggatggc ggatctgtcg
    10321 caaaccatca ccgtgatgcc gaaagcgcct aacgaagcac agatggaaac gttgctgaat
    10381 actgttggtt ttatcggcgc acagacgggc ttcccggcga ttaatctgac ggtgaccgat
    10441 gatggcagca ccattcaggg caaagatgcc gacatcatga tcatcggtgg tatcccggac
    10501 aaactgaaag acgataagca gatcgaccta ttggtgcagg cgaccgaaag ctgggtgaaa
    10561 acaccgatgc gccagacccc gttccccggc attgtgccgg acgagagcga tcgcgcggca
    10621 gaaacccagt caacgctgac ctcttccggt gcgatggcgg cggtgattgg cttccagtcg
    10681 ccgtataacg accagcgcag cgtgattgcg ctgttggcag atagcccacg cggttatgaa
    10741 atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg
    10801 gtgatccgcg agtccggtat caacagccta cgtgttggcg acgtttatta cgtaggtcat
    10861 ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg
    10921 ctggcgacta tcagtgtgat attgctggca tgggtactgt ggcgtctgct gcgaattatt
    10981 agtcgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga
    11041 atcgtgacga tgctgttgtt ggctgccttt agtgttcagg cagcctgtac ctggcctgcc
    11101 tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac
    11161 gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta tgttctttgc cctggcggct
    11221 aacgaccgtg cagctttcga taatattctc gactggacgc agaacaatct cgctcagggt
    11281 tctttaaaag aacgtttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa
    11341 gtgctggaca gcaattcggc ctccgatggt gatgtctgga tggcctggtc gttgctggag
    11401 gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt
    11461 atcgcgcggg aggaagtggt gacggtgcct gggctgggtt ccatgttgtt accgggcaaa
    11521 gtgggttttg ctgaggataa cagctggcgt tttaacccca gctacatgcc gccgacgctg
    11581 gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac caatcaacat
    11641 ttattgctgg aaaccgcccc gaaaggcttt tcgccagact gggtgcgcta tgagaaagac
    11701 aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt
    11761 tacatgtggg taggcatgat gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg
    11821 tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg
    11881 gctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcctttt
    11941 ttacaaaacc gcgatgcgca ggcagttcag cgccagcgcg tggccgataa ctttcccggc
    12001 agcgatacct attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt
    12061 ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca
    12121 cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac
    12181 caaccatcag cagaccctgc ggatggacag cggcaacact aatgccgtgc gcgggctggc
    12241 gaatatttac cgccagcagt cgccagaaaa agctgaagcg tttatcgcct cgctctctgc
    12301 cagccagcgg cgcagcattg atgatatcga acgcagcctg caaaacgacc gtctggcaca
    12361 gcaggcagag gtactggaaa accagggcaa atgggcgcag gcggcagcac ttcagcggca
    12421 acgactggcg ctgggccccg gcagcgtatg gattacttac cgactggcag gaagaagctg
    12481 cgcggcaatc caacgatggc ggcagcagtc agggcttcgg ctacacggcg cgggcattac
    12541 ttgaacgacg tgttacttcc aactggtttg ttggcacggc aattgatatc cagcaggtga
    12601 aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc ggatggcagg
    12661 gtgacatgga tttaccgccg cagccgctga taccttacgc cgactggtaa gttttcagat
    12721 agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg ggatttccgg
    12781 ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca atggtggcag
    12841 ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat ctggtccagc
    12901 agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc cgcgaaccct
    12961 tatcttcagc tattttgaaa ggcgatattc ccgaagcgga agctattctt gccagcatta
    13021 aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc taaccagttc caggcgctgc
    13081 gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg tttgagctac
    13141 cggttcaaat ctcgctgggc gtctactcgc tcgaacgtcc ggcaaatccg cagccaatag
    13201 cctatctggt gctacaggcg gattccttcc gtatgtataa gttcgtgatg agcactctct
    13261 caacgttagt gaccatttac ttacttttgt cgcttatcct gaccgtcgcc atcagctggt
    13321 gcattaaccg cctgattttg catccgttac gcaatattgc tcgcgaactt aacgccatcc
    13381 cagcccagga gcttgttggt caccaactgg cattaccgcg tctgcatcag gacgatgaaa
    13441 tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc cattatgaag
    13501 aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa gccttgctga
    13561 tggagatgct ggagcaggtt gtcgcgcgta aacaaaccac cgcgctgatg atcatcacct
    13621 gtgaaaccct gcgtgatact gcgggcgtgc tgaaagaggc gcaacgagaa attctgctgc
    13681 tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg tatgatcctc gcgcagatta
    13741 gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac gcaatcactt
    13801 tgggtcagca agtactcact atcatgagcg agcgcctgcc gattgaacgt attcaactcc
    13861 gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc gaacagcttt
    13921 acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaagaat cagattcagt
    13981 tctttgatcc gcagcagatg gaagcagccc agaagcggtt gacggaagag agcgatatcc
    14041 ttaatgcact ggaaaatcat cagtttgcaa tttggttaca gccacaggtc gagatgacca
    14101 gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat ggcagttggg
    14161 acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt accgtcggtc
    14221 actgggtgct ggaagagtcc tgtcgattgc ttgcagcctg gcaagagcgc ggcattatgc
    14281 tgcccttgtc ggtaaacctc tctgcgctgc aactgatgca cccgaatatg gtggcggata
    14341 tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg gaagtgacag
    14401 aaagccgacg tattgacgac cctcatgctg cggtggcaat cctccgtccg ctgcgcaatg
    14461 ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg cgtcagctgc
    14521 agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt gttgaaggct
    14581 tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc ctgaacttac
    14641 aaatgattgc cgaaggcgtg gagactgaag cacaacgaga ctggctggca aaagcgggcg
    14701 ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc ttcgaagaga
    14761 gttacctgga agaaaagtag ctaccccaaa ctgattacaa aactttaaaa agtgctggtt
    14821 tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca ggtacatttc
    14881 tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc ccccccccca taaccttaca
    14941 agacctgtgg ttttactaaa ggacacccta tgaaaacctc tctgtttaaa agcctttact
    15001 ttcaggtcct gacagcgata gccattggta ttctccttgg ccatttctat cctgaaatag
    15061 gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct cattaagatg atcatcgctc
    15121 ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat ggaaagcatg aaggcggtcg
    15181 gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt cagtaccatc gcgctgatta
    15241 ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg aatgaacgtc gatccggcaa
    15301 cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc gaaagaccag ggcattgtcg
    15361 ccttcattat ggatgtcatc ccggcgagcg tcattggcgc atttgccagc ggtaacattc
    15421 tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct ccaccgtctg ggcagcaaag
    15481 gccaactgat ttttaacgtg atcgaaagtt tctcgcaggt catcttcggc atcatcaata
    15541 tgatcatgcg tctggcacct attggtgcgc tcggggcaat ggcgtttacc atcggtaaat
    15601 acggcgtcgg cacactggtg caactggggc agctgattat ctgtttctac attacctgta
    15661 tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc gactggtttc agtatcttca
    15721 aatttatccg ctacatccgt gaagaactgc tgattgtact ggggacttca tcttccgagt
    15781 cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg ctgccgtaaa tcggtggtgg
    15841 ggctggtcat cccgacaggc tactcgttta accttgatgg cacatcgata tacctgacaa
    15901 tggcggcggt gtttatcgcc caggccacta acagtcagat ggatatcgtc caccaaatca
    15961 cgctgttaat cgtgttgctg ctttcttcta aaggggcggc aggggtaacg ggtagtggct
    16021 ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt gccggtagcg ggtctggcgc
    16081 tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc gctaactaac ctggtcggta
    16141 acggcgtagc gaccattgtc gttgctaagt gggtgaaaga actggaccac aaaaaactgg
    16201 acgatgtgct gaataatcgt gcgccggatg gcaaaacgca cgaattatcc tcttaatctc
    16261 accacttatg cccgcactcc cttccccgag tgcgggtgtt tgcgcataat tgacccctgt
    16321 gcctgaactt accacctggg tgagttcagg tttatttcac ttcttccgtg acattttcat
    16381 gttcttgcgg tctaacacga agtgttttta acgtcattta gactgaccga caacgggcag
    16441 tttttttatt accaggatag ttgatcaggg gttcacatgc agggcataaa aattcgactt
    16501 ttagcgggcg gtttgctgat gatggccact gctggctatg tgcaggcaga tgcgctccag
    16561 cctgatccag catggcaaca ggggacgctt tccaacggtt tacagtggca agtgctgacc
    16621 accccccagc gtcccagcga tcgtgttgaa attcgcctgc tggttaatac cggttcgctc
    16681 gccgaaagta cacaacagag cggttacagt cacgccatcc ctcgtattgc gctaacgcaa
    16741 agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc agcaggggat cgaccctaaa
    16801 cgcccgatgc cgccggtaat tgtctcttat gacaccacgc tgtttaatct gagtttgccc
    16861 aataaccgta acgatttgct gaaagaagcg ctctcttatc tggcaaatgc cactggcaaa
    16921 ttgaccatca caccagaaac catcaaccac gcgctgcaaa gtcaggacat ggtggcaacc
    16981 tggcctgccg atactaaaga gggctggtgg cgctatcgtc tgaaagggtc aaccttgtta
    17041 ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag cggaaaaaat taaagatttc
    17101 tatcagaaat ggtacacccc ggatgcaatg acgctactgg tggtgggaaa cgtggatgcg
    17161 cgctcggttg tcgaccaaat caacaaaacg tttggcgaac tgaaaggcaa acgtgaaacg
    17221 ccagctccgg tgccgacgct ttctccgctg cgtgcggaag cggtgagtat tatgactgac
    17281 gcggtgcgtc aggaccggtt atctatcatg tgggatacgc cgtggcagcc gattcgtgaa
    17341 tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc gcgaggcgct gttctggcat
    17401 gttcagcaag cgttaagcgc cagtgacagc aaagacatcg gtcttggatt tgactgccgt
    17461 gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat caccaaacga caagctgaac
    17521 agcaacctta atctggtggc gcgtgaactg gcgaaggttc gcgataaagg tctgccggaa
    17581 gaagagttca atgcgttagt ggcgcaaaag aaactggagc tgcagaaact gtttgccgcc
    17641 tatgcacggg ctgataccga tattctgatg ggccagcgga tgcgttcgtt gcaaaatcag
    17701 gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc aggatttcct taacagcctg
    17761 actgtggaga tgttaaatca ggatctgcgt cagcagttgt cgaatgatat ggcgttaata
    17821 ctgctgcagc cgaaaggcga gccggaattt aacatgaaag cgttgcaggc ggcctgggat
    17881 caaatcatgg ccccatctac cgccgctgcg accacctctg tcgccacgga tgacgtacat
    17941 cctgaagtga cggatattcc acctgcacag taatgcctga tggtgatgag ttagcacaac
    18001 catcaggtat ttatggatga gctggataga ggggtaacac tttatccagc cttttgcata
    18061 tgctgcgttt acgctggcat cgcctcacgc gggataatcg cgccgcgata ctgaataacg
    18121 gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg cactgccgcc cgtcaaacgt
    18181 accgccagat aaccggcgct gaaagagtcg ccagctgcgg tggtatcgat cactttttct
    18241 ttcggcagtt tcaccgccgg aacatcaact aacgcttcac cagcaatgga caccaggcaa
    18301 gaatccgccc cgcgtttcac caccacttct ttcacgcccg cgttatgggt gcgcgcaatg
    18361 acgtcttcca ccggctgttg accccacagc gcgtcttcgt cgtccagcgt caggaaggcg
    18421 atatccgtgc attccagcat ttgctggtac acctgctgtg tctcttcttt gctggcccac
    18481 aggcgcggac gatagttatt gtcgaaaatc acttttccgc ctttggcgcg gcattcgcgc
    18541 agcagggaaa gcagcttttc gcggctggtc gggcttaaga tcgccaggct aatcccgctc
    18601 aggtagagat aatcgaaatt cgccagctct tcgcaaatcg ccgcagactg ctcgctcgcc
    18661 agccagaatt tggcggcggc ttcgttacgc cagtagtaga acgtacgttc gccggtgctg
    18721 tcggtttcaa tgtagtaaag gcccggaaga cggttttcca tccgttgggt cagggaagta
    18781 tcaacgttct cgccgtgcca ggcgtccagc atctgctggc taaaactgtc cgttcccagc
    18841 gccgttacgt aatgaacggt taatgccgca ggatcgacct gacgggcgat atagacggaa
    18901 gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg cgcctttctc ggaaagctca
    18961 atcatgcatt cgccaatcac ggcaatcttt ttggacatag tcgtgaacct gatctgtaaa
    19021 aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa ccatattaaa acagtgttcc
    19081 attatttttt tgagccagaa cgtattcctg aaagattccg ttgtggagtg aggaaattta
    19141 ccccggccag gcggtaaagt tctgccctta cgcgccgata atctttgtcg agtccgggca
    19201 gcatcacttt taaacacagg acatctttga tgataaggca ggttatccag cgaataagca
    19261 accctgaagc aagcatcgag agcttgcagg aacggcgttt ttggttgcag tgtgagcgtg
    19321 cttacacctg gcagccgatc tatcaaacat gcgggcggtt aatggccgtg gagctattaa
    19381 cggtggtcac gcatcccttg aacccttcgc aacgcctgcc gccggatcgc tattttactg
    19441 aaatcaccgt cagccatcgg atggaggttg tgaaagagca gattgatttg ctggcgcaaa
    19501 aagccgactt ctttgtagag cacggcctgc tggcatcggt caatattgat ggccctacgc
    19561 tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat tgagcgtctt ccctggctgc
    19621 gtttcgaact ggtggagcat atccgtctgc cgaaagattc aacctttgcc tcgatgtgtg
    19681 aatttggccc gctgtggctg gatgattttg gtaccgggat ggcaaatttc tctgcactaa
    19741 gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact gtttgtgatg ctgcgtcagt
    19801 cgccggaagg ccgcacactc ttttctcagc ttttacatct aatgaatcgc tattgtcgcg
    19861 gggtgattgt cgagggcgta gaaacaccgg aagagtggcg tgatgttcag aactcgcccg
    19921 cattcgccgc acaaggctgg tttctttcac gcccggcacc gatggaaacg ctgaatacgg
    19981 cggttctggc gctataagct gcctcatttt ccgccgggct ggactatctt taggactggc
    20041 acaggaagaa tatgaggaag cgaacgatga gcaaggcagg caaaataacc gctgcgattt
    20101 caggggcttt cttgttgttg attgtcgtgg cgatcatttt gattgcaact tttgactgga
    20161 atcgactcaa accgaccatc aaccagaaag tctctgcgga gttgaatcgt ccgttcgcta
    20221 tccgtggcaa tctgggcgtg gtgtgggagc ggcaaaaaca agaaactggc tggcgcagct
    20281 gggtgccgtg gcctcatgta catgcagaag acatcattct tggtaatcca ccggatattc
    20341 ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac gctggcaccg ctggcgctgc
    20401 tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa gcccgacgcg cgcctgattc
    20461 gcctctctga aaagaacaat aactggacgt ttaatcttgc caacgatgac aacaaagacg
    20521 cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa tattcttttc gatcaagggc
    20581 ggatcgccat tgatgacaaa gtaagcaaag cggatctgga aatttttgtt gatccgttag
    20641 gcaagccgct gccattcagc gaagttactg gatcgaaagg taaagcggat aaagaaaagg
    20701 tgggcgatta catttttggc ctgaaggcgc agggacgtta taacggtgaa ccgctcacgg
    20761 gtacgggaaa aataggcggt atgctggcgc tgcgtggcga agggacgccg tttccggtac
    20821 aggctgattt ccgttcaggt aatacccgtg ttgcttttga tggcgtcgtg aatgacccaa
    20881 tgaagatggg cggtgtcgat ttacggctta aattttctgg cgattcactg ggtgatctct
    20941 atgaactgac gggcgttctg ctgcccgata ccccgccgtt tgaaacggat ggtcggctgg
    21001 tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta tcgcggtttt aatgggcgaa
    21061 ttggcgatag cgatatccac ggttctctgg tctacaccac cggaaagcca cgaccaaaac
    21121 tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga cctgggaccg ttgattggcg
    21181 ttgattccgg gaaaggggca gaaaagtcga aacggtctga acagaagaag ggcgaaaaaa
    21241 gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt cgaaaccgat aaatgggacg
    21301 ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga gcatggcagt agcctgccga
    21361 ttagcgatct ttctactcat atcatcctca aaaatgctga cctgcgcctg caaccgctga
    21421 aatttggcat ggcgggcggc agcattgcgg cgaatattca tctggaaggc gataaaaagc
    21481 cgatgcaggg gcgggcagat attcaggctc gtcgactgaa actgaaagaa ctgatgcccg
    21541 atgtggaact gatgcagaag acgctggggg aaatgaacgg tgacgcggaa ctacgcggta
    21601 gcggtaactc ggtggcggcg cttttaggca acagtaacgg caacctgaaa ctgttgatga
    21661 atgacgggct ggtgagccgc aacctgatgg agattgttgg gctgaatgtc ggcaactaca
    21721 ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa ctgcgcggcg gcgaatctga
    21781 atattgccaa cggcgtggcg cgcccgcaga tttttgcttt cgatactgag aacgcgttga
    21841 ttaatgttac cggcacggca agttttgctt cggaacagct ggatttgact attgatccgg
    21901 agagtaaagg gattcggatt atcacactgc gttcgccgct gtatgtgcgt gggacgttta
    21961 aaaatccgca ggctggggtg aaagccggac cgctgattgc ccgtggtgct gttgctgcgg
    22021 cactggcaac gctggtaaca ccggcggcgg cgttactggc actgatctca ccttccgaag
    22081 gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa gaagtgatga taattgccgg
    22141 atgtctgaca tccggcaatt aaacagattt agatttttgg caaagtgtgc tttgttcatg
    22201 ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct tgtgaattca atatattgta
    22261 tggaaattca gtcctgataa gcgtagcgca tcaggctttt cacacttatt acaacgactg
    22321 atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc aagccagcca tcgccgccag
    22381 atataacccc accgcaccta acccgtagtt agcctgcaac caggctgcga tatacggcgc
    22441 aacggaagcc ccgagaatcg acgatacgtt gtaagagaac gatgctccgg tgtaacgcac
    22501 ttctgtcgga aacagctctg gtaacagcgc acccatcggc ccgaaggtca gccccatcag
    22561 acttaacccc agcagcagga aggcaaaaac cagaatcggg ttgccagaac cgagcagcgg
    22621 gttaaaggcg aacagcgcga acaggatgat cagcgtggtg atgatcacca tgcttttacg
    22681 gcgaccaaag gcatcagcca gtaatccggc gactggcacc atcacgccaa aaccaataac
    22741 tgccatcatc agcatccaca acacttcgtt acgcggcagg ccaagcccaa ctggcgcggc
    22801 ggcggtactg aaggtcatag agtagaccgt catgatgtaa aacagcgtat aggttgccag
    22861 cataatgaac gtacccagta cggttacgcg aacatgtttg gtcagcagcg tacccagcgg
    22921 gatcttcacc tgttttttcg ctttagcgac tttctcaaac accggcgact catgcagcga
    22981 cacgcgaaca tacaggccga taatgaccag caccgccgag aagataaatg gcacacgcca
    23041 gccccagctc ataaactgct catcggtcag tagccaggaa agcagcaaga aagtgccgtt
    23101 ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag gagccataca gtgcacgttt
    23161 gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg ccccattcac cgcctaagcc
    23221 cagaccctga ccaaatcgag ccagcgccag cagcagtggg gcgaaaatac caatcgtggc
    23281 atagcccggc agcagaccaa tcaccacggt cgaaatcccc atcgttagca acgaggcgac
    23341 cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca aaaacggcag agccaatggg
    23401 gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt agcgttgctg ccgttggatc
    23461 gccctgcggg aagaagatat gcggaaacac aataacggcc gcagtggcgt aaatgtaaaa
    23521 gtcgaagaac tcaatggccg tgccaatgag agaggcgaca aggactttat tacgcgagtt
    23581 gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct gttgcttgca taatcttttc
    23641 ttattttttg gctaacgaat agccatatta cgcacagcaa aagtgatatt tcaatgtgta
    23701 acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga taatgtttag gccagtgatt
    23761 tttcttctat gaaatgcttc acataatttt ataaatagtg gataaaactg gtttcaggtt
    23821 aaataaaagt taccaactgg atttatatgc tggggttatg agttggagtg aaattgtttg
    23881 ctcccaaata gatggctgaa gaggctctgg ggaagagttt caagaggtta tgtgcgcatt
    23941 atagccggat gcgatgctgg cgcatcttat ccggcctacg gggagccagg ccgaataaaa
    24001 tcgtatgccg gataaggcgt ttacgccgca tccggcctat tttaaggctg cgttttccct
    24061 ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca aaacagcgtc
    24121 aaacgggcga agaagtagaa aaatgccatc agccccagca cggaaccaaa tgctgcgcca
    24181 gagggggatt tcatcagcga tggcagggtg taggtcatca cgattttaat cacttcaaaa
    24241 ccaatagcag ccagaaatgt tccgcgaatc agggcttttt tgcgcggacg gtggcgcggc
    24301 aaccgccaga agatccagaa gaaaagcaga tagttggcga agatggaaat cgccaggcca
    24361 atcaatcgcc acgtcggttt cagccactca atgctgttca ggtgcagggc gctaataatc
    24421 atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg tcacaatcag cgcaatcaac
    24481 aaaccaatca gtgaaataaa atcacgcaga tattttaccc agaacttctc ctgatcttgc
    24541 ggcgagcgtt cccagacatc gcgcgactgg gcacgaatcg cttcacgcag gttacccatc
    24601 cagttgatgc cggaataaag tgccaccgcc aggccgacaa gccctacagt cgtacgctgc
    24661 tgaacggcgg tgttgatggt gtttttcaac gtggcggcta gtgtcggatc gctgatgttt
    24721 tgcagaattt tgtcgaagat atcctgtagc aacatcggat gggaggccag cacaaagccc
    24781 cctgcggcaa acgacaccat caaaatcggg atcatcgaca aaaatgagaa ataggtgata
    24841 gccgcaccaa actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg aataaggtgc
    24901 gcaataaccg gttggcgttg gactttttcg gcagtggtgg tgacggtttc cagcgcctgg
    24961 ctaactttgc cgcctttctc gctattatcc aactgcttaa tcggctcgtg ctccagatcc
    25021 tgggtgggac gtttgatctc gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg
    25081 tcattcttaa aaagtatagt cggtcagtcc aggtgctctt tcattacgcc agccagccac
    25141 tgcataaaca ggtttacacg ccgggaaagc tcccgacgct gcgggtaaac cagcgaaagg
    25201 gagagcggct cggcacggta gcctggcaac acttcaataa gccgcccggc acgcagggct
    25261 tcgcgcacgg caatgcgcgg gatctgaata atccccagac cggcaagacc ggcggcgaga
    25321 tagttttcgc tactgtttac cgtcaacatg ccgccagact taaaccactg gacgccatta
    25381 acgctggcaa cctcaaaacc taacggatgt acacccaggt gcggtgtata acgcactatt
    25441 gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa agcgcgtcag atagtgcgga
    25501 ctggcacagt tgaccatggt tagtttgccg aggggacggg cgataacgcc atcttccggt
    25561 aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa gaatatctac cggacggtta
    25621 tggctactca gttccagctc aattcccgga tgctgataga gaaattccga cagacgcggc
    25681 agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc gtaatttacc gctgatactg
    25741 gtggcatcct gttgaaacag accgtccagt tcgctgagat tactcaacac atcttttgcc
    25801 cgttgataat aggtcatgcc ttctggcgtg agtttgaccc gtcgcgtagt gcgctggaga
    25861 agctgggtgc caagttgatg ttccagtgcc tgtatctggc gcgaaacact tccctttggc
    25921 aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac
    25981 tgcattgcgt gaattttatc catttctttg accgattgtt gttcacagcg aaacagtaac
    26041 gcgcatatcg cattgtttat tgttttttct aacatctaat gccgggtttc acaatgttgt
    26101 catgacgaat gtggcgattt ctcagcagga aaagggcgga atagctttat gagaaaggtg
    26161 aatcccctta aacagagcgt atttttcagt gattcccttt aaagtgaata gaggacgtat
    26221 tggctatttg tgcttatttt tgtctcattg ccatttgtta ttttggcttg tagtcgcgga
    26281 atttcatttt taattgcatg cgtactaacc accaatataa ggaaatcctc aaaattgttt
    26341 taaatagtgc cctcgacatg taatttcagg cgtttagctg taactattta tatcggtaac
    26401 tattttctac gcgtcatctc tgtagaatgg ccaccgccaa atggcgaatt cttatttttt
    26461 ctcgcgggta atacccttcc actacttaag tctttttaac gtattcaggt ggagattcag
    26521 ttatgcaaat agtcatgttt gacaggcagt caatatttat tcatggaata aaaatcagtt
    26581 tacagcagcg tattccagga gtgagtattc agggggtcag tcaggcagac gagttatggc
    26641 aaaagctgga aagttaccct gaagccttag ttatgctcga tggcgatcag gatggtgagt
    26701 tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc tgaggttaag gtgttaatca
    26761 cggcgacgga ttgcaataaa cggtggttac aggaagttat ccattttaat gtgctggcca
    26821 ttgtgcctcg cgattcaacc gtcgagacat ttgcgttggc ggtaaatagt gcggcgatgg
    26881 ggatgatgtt tctgcctgga gactggcgga cgacgccgga aaaggacatt aaagatctca
    26941 aatcgttaag cgcccgccag cgggagattt taaccatgtt agcggcgggc gaatcaaata
    27001 aggagatcgg cagagcgctg aatatcagta ccggaacggt aaaagcgcat ctggaatccc
    27061 tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc aatgatgtta aatatctcct
    27121 cctg
//
LOCUS       DAZPDE010000040        27120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPDE010000040 DAZPDE010000000
VERSION     DAZPDE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.040"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHU0J_001566"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHU0J_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR1216307.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHU0J_001567"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHU0J_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1216308.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHU0J_001568"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHU0J_001568"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1216309.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHU0J_001569"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHU0J_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHU0J_001570"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHU0J_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1216310.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHU0J_001571"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHU0J_001571"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1216311.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHU0J_001572"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHU0J_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1216312.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="ACHU0J_001573"
     CDS             7490..7639
                     /locus_tag="ACHU0J_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216313.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="ACHU0J_001574"
     CDS             7840..9423
                     /locus_tag="ACHU0J_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1216314.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHU0J_001575"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="ACHU0J_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1216315.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHU0J_001576"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="ACHU0J_001576"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1216316.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHU0J_001577"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="ACHU0J_001577"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1216317.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="ACHU0J_001578"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="ACHU0J_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHU0J_001579"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="ACHU0J_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHU0J_001580"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="ACHU0J_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHU0J_001581"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="ACHU0J_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1216318.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="ACHU0J_001582"
     CDS             20753..22168
                     /locus_tag="ACHU0J_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216319.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="ACHU0J_001583"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="ACHU0J_001583"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1216320.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="ACHU0J_001584"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="ACHU0J_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1216321.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="ACHU0J_001585"
     CDS             24478..24810
                     /locus_tag="ACHU0J_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1216322.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHU0J_001586"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="ACHU0J_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1216323.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHU0J_001587"
     CDS             complement(26660..26977)
                     /gene="yegR"
                     /locus_tag="ACHU0J_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1216324.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6186 a         7248 c         7119 g         6567 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    27061 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
//
LOCUS       DAZPDE010000041        27053 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPDE010000041 DAZPDE010000000
VERSION     DAZPDE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.041"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..176)
                     /locus_tag="ACHU0J_001588"
     CDS             complement(<1..176)
                     /locus_tag="ACHU0J_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1216325.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHU0J_001589"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHU0J_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1216326.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHU0J_001590"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHU0J_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1216327.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHU0J_001591"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHU0J_001591"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1216328.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHU0J_001592"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHU0J_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1216329.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHU0J_001593"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHU0J_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1216330.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACHU0J_001594"
     CDS             complement(5180..5422)
                     /locus_tag="ACHU0J_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1216331.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHU0J_001595"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHU0J_001595"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1216332.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHU0J_001596"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHU0J_001596"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1216333.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACHU0J_001597"
     CDS             complement(8339..9313)
                     /locus_tag="ACHU0J_001597"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1216334.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACHU0J_001598"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACHU0J_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1216335.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACHU0J_001599"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACHU0J_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1216336.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACHU0J_001600"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACHU0J_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1216337.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACHU0J_001601"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACHU0J_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1216338.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHU0J_001602"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHU0J_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1216339.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACHU0J_001603"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACHU0J_001603"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1216340.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHU0J_001604"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHU0J_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1216341.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNVGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACHU0J_001605"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACHU0J_001605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1216342.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHU0J_001606"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHU0J_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1216343.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHU0J_001607"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHU0J_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1216344.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHU0J_001608"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHU0J_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1216345.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHU0J_001609"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHU0J_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1216346.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACHU0J_001610"
     CDS             26388..26660
                     /locus_tag="ACHU0J_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1216347.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHU0J_001611"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHU0J_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1216348.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6448 a         6789 c         7229 g         6587 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattt agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatgtt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgcattt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca cta
//
LOCUS       DAZPDE010000042        25591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPDE010000042 DAZPDE010000000
VERSION     DAZPDE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.042"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHU0J_001612"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHU0J_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHU0J_001613"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHU0J_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1216349.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHU0J_001614"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHU0J_001614"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1216350.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHU0J_001615"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHU0J_001615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1216351.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHU0J_001616"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHU0J_001616"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1216352.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHU0J_001617"
     tRNA            7783..7858
                     /locus_tag="ACHU0J_001617"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACHU0J_001618"
     tRNA            8016..8091
                     /locus_tag="ACHU0J_001618"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="ACHU0J_001619"
     tRNA            8127..8202
                     /locus_tag="ACHU0J_001619"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8436..8489
                     /locus_tag="ACHU0J_001620"
     CDS             8436..8489
                     /locus_tag="ACHU0J_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216353.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHU0J_001621"
     CDS             complement(8472..9611)
                     /gene="queG"
                     /locus_tag="ACHU0J_001621"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1216354.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9610..11157
                     /gene="nnr"
                     /locus_tag="ACHU0J_001622"
     CDS             9610..11157
                     /gene="nnr"
                     /locus_tag="ACHU0J_001622"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1216355.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHU0J_001623"
     CDS             11129..11590
                     /gene="tsaE"
                     /locus_tag="ACHU0J_001623"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1216356.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11609..12946
                     /gene="amiB"
                     /locus_tag="ACHU0J_001624"
     CDS             11609..12946
                     /gene="amiB"
                     /locus_tag="ACHU0J_001624"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1216357.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12956..14803
                     /gene="mutL"
                     /locus_tag="ACHU0J_001625"
     CDS             12956..14803
                     /gene="mutL"
                     /locus_tag="ACHU0J_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1216358.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14796..15746
                     /gene="miaA"
                     /locus_tag="ACHU0J_001626"
     CDS             14796..15746
                     /gene="miaA"
                     /locus_tag="ACHU0J_001626"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1216359.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15832..16140
                     /gene="hfq"
                     /locus_tag="ACHU0J_001627"
     CDS             15832..16140
                     /gene="hfq"
                     /locus_tag="ACHU0J_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1216360.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16216..17496
                     /gene="hflX"
                     /locus_tag="ACHU0J_001628"
     CDS             16216..17496
                     /gene="hflX"
                     /locus_tag="ACHU0J_001628"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1216361.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17582..18841
                     /gene="hflK"
                     /locus_tag="ACHU0J_001629"
     CDS             17582..18841
                     /gene="hflK"
                     /locus_tag="ACHU0J_001629"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1216362.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18844..19848
                     /gene="hflC"
                     /locus_tag="ACHU0J_001630"
     CDS             18844..19848
                     /gene="hflC"
                     /locus_tag="ACHU0J_001630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1216363.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19930..20127
                     /locus_tag="ACHU0J_001631"
     CDS             19930..20127
                     /locus_tag="ACHU0J_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1216364.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20231..21529
                     /gene="purA"
                     /locus_tag="ACHU0J_001632"
     CDS             20231..21529
                     /gene="purA"
                     /locus_tag="ACHU0J_001632"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1216365.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHU0J_001633"
     CDS             21734..22159
                     /gene="nsrR"
                     /locus_tag="ACHU0J_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1216366.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22198..24639
                     /gene="rnr"
                     /locus_tag="ACHU0J_001634"
     CDS             22198..24639
                     /gene="rnr"
                     /locus_tag="ACHU0J_001634"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1216367.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHU0J_001635"
     CDS             24819..25550
                     /gene="rlmB"
                     /locus_tag="ACHU0J_001635"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1216368.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5992 a         6486 c         7185 g         5928 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaattcttc tctcaataaa
     8221 atatctacag cgacgcgatg cgttattgct ggtttttgtt gtctctgaca aactcttgta
     8281 aacagagtta tccacagcct caggctgtaa tcttaatttc aaagaaactt cgcacggtga
     8341 atggtatttt tttaacctat tgataaataa gttaaaaatc aagattccgt tttgtcgagt
     8401 cgatcacttg acgattttat tcgtcttgaa ttgcaatgcg tttttatttt tattcacaag
     8461 ctgtggatga atcaggcgtc acgcggtaac cctttttcaa tcacccgaac cagacgctgt
     8521 tttttcggca attgcacttc gactatgcac gcatttcgtc tctctatttg ctgcgcaatc
     8581 gcccacgcta tgtgctcatc gagaagtggg tgctcacctt tacgactttc cagcgctgtc
     8641 aaaatcgttt catcccaagg ggcattgcct aatgccacgg cgatattacg cagccagcgc
     8701 aggtgaccta tacggcgaat cgccgatcct tccgtgactt ttaaaaactt ctcttcgctc
     8761 caggcaaata actcaatgag ttccggtgcg tgtagcggct tacgcgggct gaaatcgtct
     8821 tctgtagtga gttgcgaata gcgattccac gggcagataa gctggcagtc atcgcaaccg
     8881 taaatacggt ttcccattaa cggtcgcaac tcttccggga gcgccccttc aagttcgatg
     8941 gtgagataag agatacagcg gcgagcatcg acggtatatg gctcgacgat ggcaccggtc
     9001 gggcaaatcg tcatacaggc cacgcatttg ccgcatcctt cctcgactgg ttgatccacg
     9061 ggcagcggaa tatcgaccag caattcgcct aaaaagaaga acgaaccggc ctcgcgattg
     9121 aggataagtg agtgcttacc tgtccagccg agcccagctt tttcagctaa cgggcgctcg
     9181 agaataggcg cagaatcgac aaacggtcta aaattcagcg aaacacaatg ttgctgaatc
     9241 atctcgccca gctttttgag tcggttgcgc agaagtttgt gatagtcacg gcccagcgca
     9301 taacggctaa catagccgag tttggggttt ttcagcgtgc tggcaaatgc ggcgttagca
     9361 ggaaggtaat tcatccgcac gctgattaca cgcagcgtac cgggtaataa ctcatggggg
     9421 cgagcgcgca gcataccgtg acgtgccatc cagtccattt cgccgtggta ttgtttgtcc
     9481 agccatgctt gcagtttggg ctcggactcg ctgagatcgg tatcggtaat acctacctgc
     9541 tgaaagccca gttccagccc ccactgttta attttttgcg ctaactgatt gagatcgagg
     9601 ggctctgaca tgacggacca tacaatgaag aaaaaccccg taagtatacc acacaccgtc
     9661 tggtacgccg acgatatccg ccgcggagaa cgcgaggcgg cagatgtgct ggggctcaca
     9721 ctctatgagc tgatgcttcg cgctggcgag gccgcattcc aggtgtgtcg ttcggcgtat
     9781 cctgacgccc gccactggct ggtgctgtgc ggtcatggta ataacggcgg cgatggctac
     9841 gtggtcgcgc gactggccaa agcggtcggc attgaggtca cgttgttggc ccaggagagc
     9901 gacaaaccgt tgccggaaga ggccgcgctg gcacgcgaag catggttaaa cgcgggtggc
     9961 gagatccatg cttcgaatat tgtctggccc gaatcggtag atctgattgt tgatgcgctg
    10021 ctcggtaccg gcttgcagca agcgccccgc gaatccatta gccagttaat cgaccacgct
    10081 aattcccatc ctgcgccgat tgtggcggtt gatatccctt ccggcctgct ggctgaaacc
    10141 ggcgctacgc caggcgcagt gatcaacgcc gatcacacca tcacttttat tgcgctgaaa
    10201 ctaggcttgc tcactggaaa agcgcgggat gttacaggac aactgcattt tgactcactg
    10261 gggctggata gctggctggc aggtcaggag acgaaaattc agcggttttc ggcagaacaa
    10321 ctttctcact ggctgaaacc gcgtcgcccg acttcgcata aaggcgatca cgggcggctg
    10381 gtgattatcg gtggcgatca cggcacggcg ggggctattc gtatgacggg ggaagcggcg
    10441 ctacgtgctg gtgctggttt agtccgagta ctgacccgca gtgagaacat tgcgccgctg
    10501 ctgactgcac gaccagaatt gatggtgcat gaactgacta tggactctct taccgaaagc
    10561 ctggaatggg ccgatgtggt ggtgattggt cccggtctgg gccagcaaga gtgggggaaa
    10621 aaagccctgc aaaaagttga gaattttcgc aaaccgatgt tgtgggatgc cgatgcattg
    10681 aacctgctgg caatcaatcc cgataagcgt cacaatcgcg tgatcacgcc gcatcctagc
    10741 gaggccgcac ggttgttagg ctgttccgtc gctgaaattg aaagtgaccg cttacattgc
    10801 gccaaacgtc tggtacaacg ttatggcggc gtagcggtgc tgaaaggtgc cggaaccgtg
    10861 gtcgccgccc atcctgacgc tttaggcatt attgatgccg gaaatgcagg catggcgagc
    10921 ggcggcatgg gcgatgtgct ctctggtatt attggcgcat tgcttgggca aaaactgtcg
    10981 ccgtatgatg cagcctgtgc aggctgtgtc gcgcacggtg cggcagctga cgtactggcg
    11041 gcgcgttttg gaacgcgcgg gatgctggca accgatctct tttccacgct acagcgtatt
    11101 gttaacccgg aagtgactga taaaaaccat gatgaatcga gtaattccgc tccctgatga
    11161 gcaggcaaca ttagacctgg gcgagcgggt agcgaaagcc tgcgatggcg caaccgtaat
    11221 ctatctgtat ggcgatttag gcgcaggtaa aaccaccttt agccggggct ttttacaggc
    11281 tctgggtcat cagggtaatg tcaaaagccc cacttatacg ctggtcgaac cctatacgct
    11341 cgacaactta atggtctatc actttgattt gtaccgcctt gccgatcccg aggagctgga
    11401 gtttatgggg atccgcgatt attttgccaa cgatgccatc tgcctggtgg agtggccaca
    11461 acaaggtaca ggtgttcttc ctgacccgga tgtcgaaata cacattgatt atcaggcaca
    11521 aggccgtgag gcgcgcgtga gtgcggtttc ctctgcgggt gaattgttgc tggcgcgttt
    11581 agccggttaa cctttgaaag gtggcgggat gatgtatcgc atcagaaatt ggttggtagc
    11641 gacgctgctg ctgctgtgca cgccggtggg tgccgcgacg ctctctgata ttcaggtttc
    11701 taacggtaat caacaggcgc ggataacgtt gagttttatt ggcgatcctg attatgcgtt
    11761 tagccatcaa agcaaacgca ccgtggcgct cgatatcaaa caaacgggca tgattcaggg
    11821 actgccgttg ttgttcagcg gcaataatct ggtgaaggcg attcgctctg gaacgcctaa
    11881 agatgcacaa acgctacggc tggtggtcga tcttaccgaa aacggtaaaa ccgaagcggt
    11941 gaagcggcag aatggcagca attacactgt cgtctttacg attaacgccg atgtgccgcc
    12001 accgcctcct ccgccgcccg tggttgcgaa acgcgttgaa acgcctgcgg ttgtcgcacc
    12061 gcgcgtcagc gaaccggcgc gcaatccgtt taaaacggaa agtaaccgca ctacgggtgt
    12121 tatcagcagt aatacggtaa cgcgtccggc agcgcgcgcg acggctaaca ctggcgataa
    12181 aattatcatc gctattgatg ccggacacgg cggtcaggac cctggcgcta tcggccccgg
    12241 tggtacgcgg gagaaaaatg tcaccatcgc catcgcgcgt aaattgcgta ctttgctcaa
    12301 tgacgatccg atgtttaaag gcgttttaac ccgtgacggg gattacttta tctcggtgat
    12361 ggggcgcagt gatgtggcac ataagcaaaa cgccaatttc ctcgtgtcga ttcacgctga
    12421 tgccgcaccg aaccgtagtg cgactggcgc ttccgtatgg gtgctctcta accgtcgcgc
    12481 caacagtgaa atggccagct ggctggagca gcacgagaaa ctgtcggagc tgctgggtgg
    12541 ggcgggtgat gtgctggcga acagtcagtc tgacccctat ttaagccagg cggtgctgga
    12601 tttacagttc ggtcattccc agcgggtagg gtatgatgta gcgaccagta tgatcagtca
    12661 gttgcaacgc attggcgaaa tacataaacg tcgaccagaa cacgccagcc ttggcgttct
    12721 gcgctcgccg gatatcccat cagtactggt cgaaaccggt tttatcagca acaacagcga
    12781 agaacgtttg ctggcgagcg acgattacca acaacagctg gcagaagcca tttacaaagg
    12841 cctgcgcaat tacttccttg cgcatccgat gcaatctgcg ccgcagggtg caacggcaca
    12901 aactgccagt acggtgacga cgccagatcg cacgctgcca aactaaggac gattgatgcc
    12961 aattcaggtc ttaccgccac aactggcgaa ccagattgcc gcaggtgagg tggtcgagcg
    13021 acctgcgtcg gtagtcaaag aactagtgga aaacagcctc gatgcaggtg cgacgcgtat
    13081 cgatattgat atcgaacgcg gtggggcgaa acttatccgc attcgtgata acggctgcgg
    13141 tatcaaaaaa gatgagctgg cgctggcgct ggctcgtcat gccaccagta aaatcgcctc
    13201 tctggacgat ctcgaagcca ttatcagcct gggctttcgc ggtgaggcgc tggcgagtat
    13261 cagttcggtt tcccgcctga cgctcacttc acgcaccgca gaacagcagg aagcctggca
    13321 ggcctatgcc gaaggacgcg acatggacgt gacggttaaa ccggcagcgc atccggtggg
    13381 aacgacgctg gaggtgctgg atctgttcta caacaccccg gcgcggcgca aattcctgcg
    13441 caccgagaaa accgaattta gccatattga tgaaatcatc cgccgcattg cgctggcgcg
    13501 tttcgacgtc acgatcaacc tgtcgcataa cggtaaaatt gtgcgtcagt accgcgcagt
    13561 gccggaaggc gggcaaaaag aacggcgctt aggcgcgatt tgcggcaccg cttttctcga
    13621 acaagcgctg gcgattgaat ggcaacacgg cgatctcacc ctgcgcggct gggtggccga
    13681 tccaaatcac actacgcccg cactggcgga aattcagtat tgctacgtga atggtcgcat
    13741 gatgcgcgat cgcctgatca atcatgcgat ccgccaggcc tgcgaagaca aactgggggc
    13801 cgatcagcaa ccggcatttg tgttgtatct ggagatcgac ccgcatcagg tggacgtcaa
    13861 cgtacacccc gccaaacacg aagtgcgttt ccatcagtcg cgtctggtgc atgattttat
    13921 ctatcagggc gtgctgagcg tgctacaaca gcaactggaa acgccgctac cgctggacga
    13981 tgaaccacaa cccgcaccgc gtgccatccc ggaaaaccgc gtggcggcgg ggcgcaatca
    14041 ctttgcagaa ccggcagctc gtgagccggt agctccgcgc tactctcctg cgcccgcctc
    14101 aggcagtcgt ccggctgccc cctggccgaa tgcgcagcca ggctaccaga aacagcaagg
    14161 tgaagtgtat cgccagcttt tgcaaacgcc cgcgccgatg caaaaaccaa aagcgccgga
    14221 accgcaggaa cctgcacttg cggcgaacag tcagagtttt ggtcgggtac tgactatcgt
    14281 ccattccgac tgtgcgttgc tggagcgcga tggcaacatt tcacttttat ccttgccagt
    14341 ggcagaacgt tggctgcgtc aggcacaatt gacgccgggt gaagcgcccg tttgcgccca
    14401 gccgctgctg attccgttgc ggctaaaagt ttctggcgaa gaaaaatcgg cattagaaaa
    14461 agcgcagtct gccctagcgg aattgggtat tgatttccag tcagatgcac agcatgtgac
    14521 catcagggcc gtgcctttac ccttacgcca acaaaattta caaatcttga ttcctgaact
    14581 gataggctac ctggcgaagc agtccgtatt cgaacctggc aatattgcgc agtggattgc
    14641 acgaaatctg atgagcgaac atgcgcagtg gtcaatggca caggccataa ccctgctggc
    14701 ggacgtggaa cggttatgtc cgcaacttgt gaaaacgccg ccgggtggtc tgttacaatc
    14761 tgttgattta catccggcga taaaagccct gaaagatgag tgatatcagt aaggcgagcc
    14821 tgcctaaggc gatttttttg atggggccga cggcctccgg taaaacggcg ttagccattg
    14881 agctgcgtaa aattttacca gtagagttga taagcgttga ttctgtcctt atttacaaag
    14941 ggatggatat cgggacggcg aagccgaacg ctgaagagtt actcgccgcg ccgcaccgat
    15001 tgctggatat tcgcgatccg tcgcaggctt actcggctgc tgattttcgc cgcgatgcgc
    15061 tggcggaaat ggccgatatc accgcggcgg ggcggatccc actgttagtg ggcggtacga
    15121 tgttgtattt caaggcattg ctggaagggt tgtcgccgct accgtcggca gacccggaag
    15181 tgcgggccag aattgagcaa caggcggcag agcaaggttg ggagtcattg catcgtcaac
    15241 ttcaggaggt agatccggtt gcggcagcaa ggattcatcc aaatgatcca caaaggcttt
    15301 cccgggcact ggaagttttt ttcatttcgg gtaaaacttt aacggaactg acgcaaacgt
    15361 caggagacgc tctaccgtat caggtgcatc agttcgccat cgccccggcg agccgtgaac
    15421 tgctccatca acgcattgag cagcgttttc atcagatgtt ggcttcaggt tttgaagcag
    15481 aagtccgggc gctttttgcc cgaggagatt tgcatacgga cttgccttcc attcgttgcg
    15541 tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat ctcatacgat gaaatggttt
    15601 atcgaggtgt ttgcgccacg agacagttgg cgaagcggca gataacctgg ctgcgtggtt
    15661 gggaaggggt tcactggctt gacagtgaaa aaccagaaca ggcgcgtgac gaagtattac
    15721 aggttgttgg tgctatcgca ggctgaatgt gtacaattga gacgtatcgt gcgcaatttt
    15781 ttcagaatcg aaaggttcaa agtacaaata agcatataag gaaaagagag aatggctaag
    15841 gggcaatctt tacaagatcc gttcctgaac gcactgcgtc gggaacgtgt tccagtttct
    15901 atttatttgg tgaatggtat taagctgcaa gggcaaatcg agtcttttga tcagttcgtg
    15961 atcctgttga aaaacacggt cagccagatg gtttacaagc acgcgatttc tactgttgtc
    16021 ccatctcgcc cggtttctca tcacagtaac aacgccggtg gcggtaccag cagtaactac
    16081 catcatggta gcagcgcgca gaatacttcc gcgcaacagg acagcgaaga aaccgaataa
    16141 ggtttcgggc tgttttttta cacggggagc cagcgatcct gcgttccccg ctgatctatt
    16201 tagagggtta tacgcttgtt tgaccgttat gatgctggtg agcaggcggt actggtacac
    16261 atctatttta cgcaagacaa agatatggaa gacctccagg agtttgaatc tctggtctct
    16321 tccgccggtg tcgaagcatt gcaggtgatt accggtagcc gtaaagcgcc gcacccaaag
    16381 tattttgtag gtgaaggtaa agcagttgaa attgcggaag ctgtcaaagc gatgggtgct
    16441 tcggtcgttc tttttgacca tgccctgagc ccggcgcaag agcgtaacct ggagcgtttg
    16501 tgcgagtgtc gtgttatcga ccgcaccggc cttattttag atattttcgc ccaacgtgcg
    16561 cgtacccatg agggtaagtt gcaggttgag ctggcgcagc tgcgccatct ggctacgcgc
    16621 ctggtgcgtg gctggaccca ccttgaaaga cagaaaggcg ggataggttt gcgtggtccg
    16681 ggtgaaaccc agctcgaaac cgaccgtcgt ttgttgcgta atcgcatcgt gcagatacag
    16741 tcgcgcctgg aaagagttga aaagcagcgt gagcaggggc ggcaatcgcg tatcaaagcc
    16801 gacgttccta ctgtttcgct ggtgggatat accaacgccg gtaaatctac ccttttcaat
    16861 cgcatcaccg aagcgcgggt ctacgcggca gaccagttgt ttgccaccct cgacccgacg
    16921 ttgcggcgta ttgacgttgc agatgtcggt gaaaccgtac ttgcagatac cgtagggttt
    16981 attcgccacc tgccgcacga tctggtggcg gcatttaaag ccacgttaca agagacgcgg
    17041 caagccacat tactgctgca cgtcattgat gcggcggatg tgcgtgtaca agaaaacatc
    17101 gaagcggtga atacggttct tgaagagatc gacgctcacg agatcccaac cctgctggtg
    17161 atgaacaaga tcgatatgct ggaagatttc gaaccgcgta ttgatcggga cgaagagaac
    17221 aaaccgatcc gtgtctggct ttccgcacag accggagcgg ggataccaca gctttttcag
    17281 gctttgacgg agcggctttc cggcgaggtg gcgcagcata cattgcgtct gccaccgcag
    17341 gaagggcgtc tgaggagtcg tttttatcag cttcaggcaa tagaaaaaga gtggatggag
    17401 gaggacggca gcgtaagtct gcaagttcgt atgccgatcg ttgactggcg tcgcctctgt
    17461 aaacaagaac cggcgttgat cgattacctg atctaacggc gtagcgtctg aagcgtggag
    17521 tcatatcctc tggcgtcgaa agacaacagg gatcaccgca taacaaatat ggagcacaaa
    17581 catggcgtgg aatcagcccg gtaataacgg acaagaccgc gacccgtggg gaagcagcaa
    17641 acctggcggc aactctgagg gaaatggaaa caaaggcggt cgcgatcaag ggccacctga
    17701 tttagatgat atcttccgca aactgagcaa aaagctcggt ggtctgggcg gcggtaaagg
    17761 caccggatct ggcggtggca gttcatcgca aggcccgcgc ccgcagcttg gcggtcgtgt
    17821 cgttaccatc gcagcggcag cgattgtcat tatctgggcg gccagtggtt tctataccat
    17881 taaagaagcc gaacgcggcg tggtaacacg ctttggtaaa ttcagccatc tggttgagcc
    17941 gggtctgaac tggaaaccga cgtttatcga cgaagtcaaa ccggtgaacg tggaagccgt
    18001 gcgtgaactg gccgcttctg gtgtgatgct gacgtcggac gagaacgtag tgcgcgttga
    18061 gatgaacgtg cagtaccgcg tcaccaatcc ggaaaaatat ctgtatagcg tgaccagccc
    18121 ggatgacagc ctgcgtcagg ctaccgacag cgccctgcgt ggagttatcg gtaaatacac
    18181 catggaccgc attctgacgg aaggtcgtac cgtgattcgt agcgatactc agcgcgaact
    18241 ggaagagacg attcgtccgt atgacatggg tatcacgctg ctggacgtca acttccaggc
    18301 tgctcgtccg ccggaagaag taaaagcggc gtttgacgat gcgattgccg cgcgtgaaaa
    18361 cgaacagcaa tacattcgtg aagcagaagc gtattccaac gaagttcagc cgcgtgcgaa
    18421 cggtcaggcg caacgtatcc tcgaagaggc gcgtgcgtac aaggcccaga ccatcctgga
    18481 agctcagggt gaagtggcgc gctttgctaa acttctgccg gaatataaag ccgcgccgga
    18541 aattactcgc gagcgtctgt atatcgagac gatggaaaaa gtgttgggta acacccgcaa
    18601 agtgctggtt aacgataaag gtggcaacct gatggttctg ccgttagacc agatgctgaa
    18661 aggtggtaac gcccctgcgg cgaagagcga taacggtgcc agcaatctgc tgcgtctgcc
    18721 gccagcctct tcctccacaa ccagtggagc aagcaacacg tcgtccacca gtcagggcga
    18781 tattatggac caacgccgcg ccaacgcgca gcgtaacgac taccagcgtc agggggaata
    18841 acgatgcgta agtcagttat cgcgattatc atcatcgtgc tggtagtgct ttacatgtct
    18901 gtctttgtcg tcaaagaagg tgagcgcggt attacgctgc gttttggtaa ggtactgcgt
    18961 gacgatgaca acaaaccttt ggtttatgag cctggtctgc atttcaagat accgttcatt
    19021 gaaacggtga aaatgctcga cgcacgtatt cagaccatgg acaaccaggc cgaccgcttt
    19081 gtgaccaaag agaagaaaga cctgatcgtc gactcttaca tcaaatggcg catcagcgat
    19141 ttcagccgtt actacctggc aacgggtggt ggcgacattt cgcaagcgga agtgctgttg
    19201 aaacgtaagt tctctgaccg tctgcgttct gaaattggtc gcctggacgt gaaagatatc
    19261 gtcaccgatt cccgtggtcg tctgaccctc gaagtacgtg acgcgctgaa ctccggttct
    19321 gcgggtacag aagatgaagt tactaccccg gcagcagata acgccattgc cgaagcggca
    19381 gagcgcgtaa cggctgagac gaagggcaaa gttccggtca tcaacccgaa cagtatggcg
    19441 gcgctgggta ttgaagttgt cgatgtgcgt atcaagcaga tcaacctgcc gaccgaagtg
    19501 tctgaagcga tctacaaccg tatgcgcgcc gagcgtgaag cggtagcgcg tcgtcaccgt
    19561 tcacaaggtc aggaagaagc ggaaaaactg cgcgcgactg ccgactatga agtgaccaga
    19621 acgctggcag aagctgagcg tcagggccgc attatgcgtg gtgaaggcga tgccgaagca
    19681 gccaaactgt ttgctgatgc attcagtaaa gatccggact tctacgcatt catccgtagc
    19741 ctgcgtgctt atgagaacag cttctctggc aatcaggacg tgatggtcat gagcccggat
    19801 agcgatttct tccgctacat gaagacgccg acttccgcaa cgcgttaata taacgactgc
    19861 ggtacaggtc aataaagcca ccgcatcctc agggatgtcg gtggttttct ttttctataa
    19921 ggataatgaa tgaattcgac aatctggctg gcgcttgccc tggttttggt actggaaggt
    19981 ttagggccga tgctttaccc gaaggcatgg aagaagatga tctctgcgat gaccaatttg
    20041 cccgataata ttttacgtcg ttttggcggt ggacttgtgg ttgcgggcgt tgtggtctac
    20101 tacatgttga ggaaaacgat tggctgaaca aaaaacagac tgatcgaggt catttttgag
    20161 tgcaaaaagt gctgtaactc tgaaaaagcg atggtagaat ccatttttaa gcaaacggtg
    20221 attttgaaaa atgggtaaca acgtcgtcgt actgggcacc caatggggtg acgaaggtaa
    20281 aggtaagatc gtcgatcttc tgactgaacg ggctaaatat gttgtacgct accagggcgg
    20341 tcacaacgca ggccatactc tcgtaatcaa cggtgaaaaa accgttctcc atcttattcc
    20401 atcaggtatt ctccgcgaga atgtaaccag catcatcggt aacggtgttg tgctgtctcc
    20461 ggccgcgctg atgaaagaga tgaaagaact ggaagaccgt ggcatccccg ttcgtgagcg
    20521 tctgctgctg tctgaagcat gtccgctgat ccttgattat cacgttgctc tggataacgc
    20581 gcgtgagaaa gcgcgtggcg cgaaagcgat cggcaccacc ggtcgtggta tcgggcctgc
    20641 ttatgaagat aaagtggcac gtcgcggtct gcgtgttggc gaccttttcg acaaagaaac
    20701 cttcgctgaa aaactgaaag aagtgatgga atatcacaac ttccagttgg ttaactacta
    20761 caaagctgaa gcggttgatt accagaaagt tctggatgat acgatggctg ttgccgacat
    20821 cctgacttct atggtggttg acgtttctga cctgctcgac caggcgcgtc agcgtggcga
    20881 tttcgtcatg ttcgaaggtg cgcagggtac gctgctggat atcgaccacg gtacttatcc
    20941 gtacgtaact tcttccaaca ccactgctgg tggcgtggcg accggttccg gcctgggccc
    21001 gcgttatgtt gattacgttc tgggtatcct caaagcttac tcaactcgtg taggtgcagg
    21061 tcctttcccg gccgaactgt ttgatgaaac tggcgagttc ctctgcaagc agggtaacga
    21121 attcggtgca actacgggtc gtcgtcgtcg taccggctgg ctggacaccg ttgccgttcg
    21181 tcgtgcggta cagctgaact ccctgtctgg cttctgcctg actaaactgg acgttctgga
    21241 tggcctgaaa gaggttaaac tctgcgtggc ttaccgtatg ccggatggtc gcgaagtgac
    21301 taccactccg ctggcagctg acgactggaa aggtgtagag ccgatttacg aaaccatgcc
    21361 gggctggtct gaatccactt tcggcgtgaa agatcgtagc ggcctgccgc aggcggcgct
    21421 gaactacatc aagcgtattg aagagctgac cggtgtgccg atcgatatca tctctaccgg
    21481 tccggatcgt actgaaacca tgattctgcg cgacccgttc gacgcgtaat tctggtacgc
    21541 ctggcagata ttttgcctgc cgggctaaca gtgtgataca ttgctgtgtc ggttaagcca
    21601 ttacgctatc cgacacagtg ttaaatcctc gcttttttcc ttccccgaac tgaaataaat
    21661 tagcgacaca gcttgtggct ggtttatcat caatataaat gtattttttc ccgatttccc
    21721 ttttgaggtt gatgtgcagt taacgagttt cactgattac ggattacgtg cgctgatcta
    21781 catggcgtca ttgccagaag ggcggatgac cagtatttct gaagtgactg acgtctacgg
    21841 cgtctcccgt aatcatatgg tcaaaataat caatcaactt agtcgtgccg gctacgtgac
    21901 tgctgttcgt ggaaaaaatg gcggcattcg cctgggtaaa ccggcgagtg cgatacgtat
    21961 tggtgatgtg gtgcgcgagc tggagccctt atcgctggtg aattgcagca gtgagttttg
    22021 ccacattaca cctgcctgtc ggttgaaaca ggcgctttct aaggccgtgc aaagttttct
    22081 tacggaactg gataactaca cgcttgccga tttggttgaa gagaatcaac cgctttataa
    22141 attattgctg gtggagtgac gaaaatcttc atcagagatg acaacggagg aaccgagatg
    22201 tcacaagatc ctttccagga acgcgaagct gaaaaatacg cgaatcccat ccctagtcgg
    22261 gaatttatcc tcgaacattt aaccaaacgt gaaaaaccgg ccagccgtga tgagctggcg
    22321 gtagaactgc acattgaagg cgaagagcag cttgaaggcc tgcgtcgccg cctgcgcgcg
    22381 atggagcgcg atggtcaact ggtcttcact cgtcgtcagt gctatgcgct gccggaacgc
    22441 ctcgacctgg tgaaaggtac cgttattggt caccgtgatg gctacggctt tctgcgggtt
    22501 gaagggcgta aagatgattt gtatctctcc agcgagcaga tgaaaacctg cattcatggc
    22561 gatcaggtgc tggcgcagcc gctgggtgct gaccgtaaag gtcgtcgtga agcgcgtatt
    22621 gtccgcgtac tggtgccaaa aaccagccag attgttggtc gctactttac cgaagcgggc
    22681 gtcggctttg tggttcctga cgatagccgt ctgagcttcg atatcttaat cccgcccgat
    22741 cagatcatgg gcgcgcggat gggctttgtg gtcgtagtcg aactgactca gcgtccgact
    22801 cgccgcacca aagcggtggg taaaatcgtc gaagtgctgg gcgacaatat gggcaccggc
    22861 atggcggttg atatcgctct gcgtacccat gaaattccgt atatctggcc gcaggctgtt
    22921 gagcaacagg ttgcggggct gaaagaagaa gtgccggaag aagcaaaagc gggccgtgtc
    22981 gatttgcgcg atttaccgct ggtcaccatt gatggcgaag acgcccgtga ctttgacgat
    23041 gcagtttact gcgagaaaaa acgcggcggc ggctggcgtt tatgggtcgc gattgccgac
    23101 gtcagctact atgtgcgtcc gccaacgccg ctggacagag aagcgcgtaa ccgtggcacg
    23161 tcggtgtact tcccttcgca ggttatcccg atgctgccgg aagtgctctc taacggcctg
    23221 tgttcgctca acccgcaggt agaccgcctg tgtatggtgt gcgagatgac ggtttcgtcg
    23281 aaaggccgcc tgacgggcta caaattctac gaagcggtga tgagctctca cgcgcgtctg
    23341 acctacacca aagtctggca tattctgcag ggcgatcagg atctgcgcga gcagtacgcc
    23401 ccgctggtta agcatctcga agagttgcat aacctctata aagtgctgga taaagcccgt
    23461 gaagaacgcg gtgggatctc atttgagagc gaagaagcga agttcatttt caacgctgaa
    23521 cgccgtattg aacgtatcga acagacccag cgtaacgacg cgcacaaatt aattgaagag
    23581 tgcatgattc tggcgaatat ctcggcggcg cgtttcgttg agaaagcgaa agaaccggca
    23641 ctgttccgta ttcacgacaa gccgagcacc gaagcgatta cctctttccg ttcagtgctg
    23701 gcggagctgg ggctggagct gccgggtggt aacaagccgg aaccgcgtga ctacgcggaa
    23761 ctgctggagt cggttgccga ccgtcctgat gcagaaatgc tgcaaaccat gctgctacgc
    23821 tcgatgaaac aggcgattta cgatccagaa aaccgtggtc acttcggtct ggcattgcag
    23881 tcctatgcgc actttacttc gccgattcgt cgttatcctg acctgacgct gcaccgcgcc
    23941 attaaatatc tgctggcgaa agagcagggg catcagggca acaccactga aaccggcggc
    24001 taccattatt cgatggaaga gatgttgcaa ctgggtcagc actgttcgat ggcggaacgt
    24061 cgtgccgacg aagcaacgcg cgatgtcgct gactggctga agtgtgactt catgctcgac
    24121 caggtaggta acgtctttaa aggcgtaatt tccagcgtca ctggctttgg cttcttcgtc
    24181 cgtctggacg acttgttcat tgatggtctg gtccatgtct cttcgctgga caatgactac
    24241 tatcgctttg accaggtagg gcaacgcctg atgggggaat ccagcggcca gacttatcgc
    24301 ctgggcgatc gcgtggaagt tcgcgtcgaa gcggttaata tggacgagcg caaaatcgac
    24361 tttagtctga tctccagtga acgcgcaccg cgcaacgtcg gtaaaacggc gcgcgagaaa
    24421 gcgaaaaaag gcgatgcagg caaaaaaggc ggcaagcgtt gtcaggtcgg taaaaaggta
    24481 aactttgagc cagacagcgc cttccgcggt gagaaaaaaa cgaagccgaa agcggcgaag
    24541 aaagacgcga gaaaagcgaa aaagccatcg gcgaaaacgc agaaaatagc cgcagcgacc
    24601 aaagcgaagc gtgcggcgaa gaaaaaagtg gcagagtgat caataccctc tttaaaagaa
    24661 gagggttaga ttgctgacaa aatgcgcttt gttcatgccg gatgcggcgt gaacgcctta
    24721 tccggcctac ataatcacgc aaattcaata tattgcagag atcatgtagg ccggataaac
    24781 gtagtgcatc aggcaaaacg taaacaacga gtacattaat gagcgaaatg atttacggca
    24841 tccacgcagt gcaggccctg ctggagcgcg cccctgaacg ttttcaggaa gtctttattt
    24901 taaaaggccg tgaagataaa cgtctgttac cgctgattca cgcccttgag tctcagggcg
    24961 tggttatcca gttggcaaac cgccaatatc tcgacgagaa aagcgacggt gccgtgcatc
    25021 agggcattat cgcccgcgtg aagccaggac gtcagtatca ggaaaacgat ctgccggatc
    25081 tgatcgcttc gctcgatcaa ccgttcctgc tgatcctcga cggcgtaacc gatccgcaca
    25141 acctcggcgc gtgcctgcgt agtgcggacg ccgcaggcgt tcatgcggtg attgtgccga
    25201 aagatcgctc cgcacagctc aacgctacgg cgaaaaaagt agcctgcggc gcggcagaaa
    25261 gcgttccgct gattcgggtg actaaccttg cgcgcaccat gcgtatgttg caggaagaga
    25321 atatctggat cgtcggtacg gcaggcgagg cggatcatac actctatcag agcaaaatga
    25381 ccggacgcct ggcgctggtg atgggcgcgg aaggtgaagg tatgcgtcgc ctgacccgtg
    25441 aacattgcga tgagttgatc agcatcccaa tggcaggaag tgtttcttcc ctgaacgttt
    25501 cggttgcaac cggaatttgc ttatttgaag cggttcgtca gcgcagctag ttcctctgaa
    25561 ttatgaaaag ccatccagat ttggatggtt t
//
LOCUS       DAZPDE010000043        25081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPDE010000043 DAZPDE010000000
VERSION     DAZPDE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.043"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            144..2372
                     /gene="crfC"
                     /locus_tag="ACHU0J_001636"
     CDS             144..2372
                     /gene="crfC"
                     /locus_tag="ACHU0J_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1216369.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2369..3247
                     /locus_tag="ACHU0J_001637"
     CDS             2369..3247
                     /locus_tag="ACHU0J_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR1216370.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3511..5013
                     /gene="proP"
                     /locus_tag="ACHU0J_001638"
     CDS             3511..5013
                     /gene="proP"
                     /locus_tag="ACHU0J_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1216371.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5125..5214
                     /gene="pmrR"
                     /locus_tag="ACHU0J_001639"
     CDS             5125..5214
                     /gene="pmrR"
                     /locus_tag="ACHU0J_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1216372.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5190..6290)
                     /gene="pmrB"
                     /locus_tag="ACHU0J_001640"
     CDS             complement(5190..6290)
                     /gene="pmrB"
                     /locus_tag="ACHU0J_001640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1216373.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6291..6959)
                     /gene="basR"
                     /locus_tag="ACHU0J_001641"
     CDS             complement(6291..6959)
                     /gene="basR"
                     /locus_tag="ACHU0J_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1216374.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6956..8599)
                     /gene="eptA"
                     /locus_tag="ACHU0J_001642"
     CDS             complement(6956..8599)
                     /gene="eptA"
                     /locus_tag="ACHU0J_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1216375.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8703..10040)
                     /gene="adiC"
                     /locus_tag="ACHU0J_001643"
     CDS             complement(8703..10040)
                     /gene="adiC"
                     /locus_tag="ACHU0J_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1216376.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10177..10938)
                     /gene="adiY"
                     /locus_tag="ACHU0J_001644"
     CDS             complement(10177..10938)
                     /gene="adiY"
                     /locus_tag="ACHU0J_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1216377.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11263..13530)
                     /gene="adiA"
                     /locus_tag="ACHU0J_001645"
     CDS             complement(11263..13530)
                     /gene="adiA"
                     /locus_tag="ACHU0J_001645"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1216378.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13729..14637)
                     /gene="melR"
                     /locus_tag="ACHU0J_001646"
     CDS             complement(13729..14637)
                     /gene="melR"
                     /locus_tag="ACHU0J_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1216379.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14920..16275
                     /gene="melA"
                     /locus_tag="ACHU0J_001647"
     CDS             14920..16275
                     /gene="melA"
                     /locus_tag="ACHU0J_001647"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1216380.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            16378..17799
                     /gene="melB"
                     /locus_tag="ACHU0J_001648"
     CDS             16378..17799
                     /gene="melB"
                     /locus_tag="ACHU0J_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1216381.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17938..18567)
                     /locus_tag="ACHU0J_001649"
     CDS             complement(17938..18567)
                     /locus_tag="ACHU0J_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1216382.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(18690..20336)
                     /gene="fumB"
                     /locus_tag="ACHU0J_001650"
     CDS             complement(18690..20336)
                     /gene="fumB"
                     /locus_tag="ACHU0J_001650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1216383.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(20414..21754)
                     /gene="dcuB"
                     /locus_tag="ACHU0J_001651"
     CDS             complement(20414..21754)
                     /gene="dcuB"
                     /locus_tag="ACHU0J_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1216384.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22325..23044)
                     /gene="dcuR"
                     /locus_tag="ACHU0J_001652"
     CDS             complement(22325..23044)
                     /gene="dcuR"
                     /locus_tag="ACHU0J_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1216385.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(23041..24672)
                     /locus_tag="ACHU0J_001653"
     CDS             complement(23041..24672)
                     /locus_tag="ACHU0J_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1216386.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24853..24990
                     /gene="yjdI"
                     /locus_tag="ACHU0J_001654"
                     /pseudo
     CDS             24853..24990
                     /gene="yjdI"
                     /locus_tag="ACHU0J_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
BASE COUNT         6056 a         6089 c         6496 g         6440 t
ORIGIN      
        1 agaagacgtt atttattctg ataaaaaaac aacacactaa atataaaatt atttcttgca
       61 gtaattataa aagccgaaaa cagaactcaa aaacaatctg gctaaataaa agtaacaaaa
      121 tttgctttaa ggaagaattt tctatgtaca cacagaccct gtatgagtta agtcaggagg
      181 ctgaacgcct gttacagctt tctcgccaac agttgcagtt actggaaaaa atgcctctct
      241 ctgtacccgg agacgacgcg ccacaactgg ctttaccctg gagtcagcct aatatcgccg
      301 aacgtcacgc gatgctgaat aatgagttgt gtaaaatttc ccgactggaa atggtgctgg
      361 caattgtcgg taccatgaaa gcagggaaat caaccaccat taatgccatt gttggtacgg
      421 aggttctgcc taatcgtaat cgcccaatga ctgcgctgcc gacgcttatt cgccatacgc
      481 ccgggcaaaa ggaaccggta ctgcattttt cacatgtcgc gccaatcgat tgtttaattc
      541 aacaattaca acagcgcctg cgtgattgcg atattaagca tctgaccgat gtgctggaaa
      601 tagataaaga tatgcgtgcg cttatgcagc ggatcgaaaa tggcgtcgct ttcgaaaaat
      661 attatctggg tgcccagcct atttttcatt gtctgaaaag tttgaatgat ttagtgcgac
      721 tggcgaaggc gctggacgtc gattttcctt tttctgctta cgccgccatt gagcatattc
      781 ccgtgattga agtggagttt gtccatctgg cggggctgga aagttatccc ggtcagttga
      841 cgttactgga tacccccggg ccaaatgaag ccgggcaacc gcatctgcaa aaaatgctta
      901 accagcagct ggcacgcgcc tcggcggtac tggcggtgct ggattatacg caactgaaat
      961 cgatctccga tgaagaggtc cgtgaggcga ttttggcggt ggggcaatcg gtgccgctgt
     1021 atgtgctggt caataagttc gatcaacagg atcgtaacag tgacgacgcc gaccaggtgc
     1081 gggcactgat ttccgggacg ctgatgaaag gctgtattac gccacagcag atattcccgg
     1141 tgtcgtcgat gtggggctac ctggcgaatc gggcgcgcta tgagttagcc aacaacggta
     1201 agttaccagc gccagagcaa caacgctggg tggaagattt tgcccatgcc gcgctcggca
     1261 ggcgctggcg tcatgccgat ctggcggacc tcgaacatat tcgtcatgct gccgatcagt
     1321 tgtgggaaga ttcgctgttc gcccagccaa ttcaggcgtt gcttcatgcc gcttacgcta
     1381 acgcctcatt gtatgctctg cgatctgctg cgcataaact gttgaattac gcgcagcagg
     1441 cgcgggaata cctggatttt cgtgcgcacg ggttaaacgt cgcttgtgaa caattgcggc
     1501 aaaatatcca tcaggtcgaa gaaagtttgc agctattgca actcaatcag gcgcaggtga
     1561 gcggcgagat taaacatgaa atcgagctgg ccctgacctc cgccaaccac tttctgcgtc
     1621 aacagcaaga tgcggtgaat gcgcagttag ccgccttgtt tcaggatgat tcggagccgt
     1681 taagcgagat tcgtacccgc tgtgagacac tgttacagac ggcgcagaac accatcagtc
     1741 gcgactttac gctgcgtttt gccgagatgg aatccaccct ttgccgggtg ttaaccgatg
     1801 ttattcgccc cattgagcaa caagtcaaaa tggaattgag cgagtcaggg tttcgtcctg
     1861 ggtttcattt tcctgttttt cacggcgtag ttccccactt caacactcgc cagctgttca
     1921 gtgaagtcat ttcgcgccag gaagcaacgg acgagcagag cacgcgttta ggcgttgtgc
     1981 gtgagacttt ttcgcgctgg ttgaatcagc ccgactgggg acggggaaat gagaaatccc
     2041 cgacagaaac ggttgattac agtgtgttgc aacgagcatt aagcgcagaa gtcgatctct
     2101 attgccaaca aatggctaaa gttctggcag agcaggtcga tgaatctgtt acggcaggca
     2161 tgaatacttt tttcgctgag ttcgcttcat gtttgacaga attacagacg cgtttacgcg
     2221 aaagtctggc tctgcgtcaa caaaatgaat cggtggtcag gctgatgcag cagcaattgc
     2281 agcagactgt gatgactcac agctggattt acaccgacgc tcagctgtta cgcgatgata
     2341 ttcaaacact tttcacggca gaacgatatt gaccaagacg ttactggacg gccccggtcg
     2401 cgtgctggag tcggttcatc cgcgcttttt agtggatctg gcgcagggcg atgatgcccg
     2461 ccatcctcaa gcccatcagc agcagtttcg tgaacgactg atgcaggaac ttctttcgcg
     2521 tgtgcagctt cagacatgga cgaacggcgg catgttaaat gcgccgctta gcctgcgact
     2581 gacaatggtg gaaaaactgg cgtcgatgct ggatcccggt catctggcgc tgacgcagat
     2641 cgcgcagcat ctggcgctgc tgcaaaaaat ggatcaccgc cagcactctg ctttcccgga
     2701 gctcccccag caaattgccg ccttgtatga gtggttttca gcccgttgtc gctggaagga
     2761 aaaggcgtta acgcaacgag gcctactggt gcaggcaggt gatcagagcg agcaaatttt
     2821 tacccgctgg cgtgctgggg cgtataacgc ctggtcgttg cctgggcgct gttttatcgt
     2881 tctggaggag ttgcgctggg gtgcatttgg cgatgcctgc cgtctgggaa gcccgcaagc
     2941 ggtggtgttg ttgctgggtg atttgcgcga gaaagcgaca caacatctgg cagagagtat
     3001 caatgcggca ccgactacgc gtcactatta ccatcagtgg tttgcctctt cgaccgttcc
     3061 gacgggcggg gatcatgctg attttttaag ttggctggga aagtggacca cggcagataa
     3121 acaacccgtt tgctggtcag tgacccaacg ctggcaaact gtcgcgctgg ggatgccacg
     3181 actctgttca gcgcagcgtt tggtgggggc aatggtcgag gaaatcttct ctgtaaattt
     3241 ggcgtaaata atcagttaca tcaatgagtc ctaaacgaaa tccatgtgtg aagttgatca
     3301 caaatttaaa cactggtagg gtaaaaaggt cattaactgc ccaattcagg cgtcaactgg
     3361 tttgattgta cattccttaa ccggagggtg taagcaaacc cgctacgctt gttacagaga
     3421 ttgcatcctg caattcccgc tccccttttg cggccgtcgc gctgattttt ctggcgtttg
     3481 cggaaatggg ccaactctgc gaggaaagct atgctgaaaa ggaaaaaagt aaaaccgatt
     3541 acccttcgtg atgtcaccat tattgatgac ggtaaactgc gtaaagccat taccgcagca
     3601 tcactgggta atgcaatgga atggtttgat tttggtgttt atggttttgt tgcttacgca
     3661 ttaggtaaag tttttttccc gggggctgac cccagcgtgc agatggttgc tgcacttgcc
     3721 actttctccg ttccctttct gattcgaccg cttggcggac tcttctttgg tatgttgggc
     3781 gataaatatg gtcgccagaa gatcctcgct atcactattg tgattatgtc gatcagtacg
     3841 ttctgtattg gcttaatacc gtcctacgac acgattggta tttgggcacc gattctgctg
     3901 ttgatctgta agatggcaca aggtttctcg gtcggtggtg aatataccgg ggcgtcgata
     3961 tttgttgcgg aatactcccc tgaccgtaaa cgtggcttta tgggcagctg gctggacttc
     4021 ggttctattg ccgggtttgt gctgggtgcg ggcgtggtgg tgttaatttc gaccattgtc
     4081 ggcgaagcga acttcctcga ctggggctgg cgtattccgt tctttattgc tctgccgtta
     4141 gggattatcg ggctttacct gcgccatgcg ctggaagaaa ctccggcgtt ccagcagcat
     4201 gttgataaac tggaacaggg cgaccgcgaa ggtttgcagg atggcccgaa agtctcgttt
     4261 aaagagattg ccactaaata ctggcgcagc ctgttgacat gtattggtct ggtaattgcc
     4321 accaacgtga cttactacat gttgctgacc tatatgccga gttatttgtc gcataacctg
     4381 cattactccg aagaccacgg ggtgctgatt attatcgcca ttatgatcgg tatgctgttt
     4441 gtccagccgg tgatgggctt gctgagtgac cgttttggcc gtcgtccgtt tgtgctactt
     4501 ggtagtgttg cactgtttgt gttggcgatc ccggcgttta ttctgattaa cagtaacgtc
     4561 atcggcctga tttttgccgg gttactgatg ctggcggtga tccttaactg ctttatgggc
     4621 gttatggctt ctaccttgcc agcgatgttc ccgacgcata tccgttacag cgcgctggcg
     4681 gcggcattta atatttcggt gctggttgcc ggtctgacgc caacactggc ggcctggctg
     4741 gtcgaaagct cgcagaatct gatgatgcct gcctattacc tgatggtagt ggcggtgatt
     4801 ggtttaatca ccggcgtaac catgaaagag acggcaaatc gtccgttgaa aggtgcgaca
     4861 ccggcggcgt cagatataca ggaagcgaag gaaattctcg tcgagcatta cgataatatc
     4921 gagcagaaaa tcgataatat tgaccacgag attgccgatt tgcaggcgaa acgtacccgc
     4981 ctggtgcagc aacatccgcg aattgatgaa taagctgaaa cggatggcct gatgtgacgc
     5041 tgtcttatca ggccaattga actcttaagg ttcacttaat ctctgacgcg catactctcc
     5101 tccaggttaa cggaggagag tgcaatgaaa aaccgtgttt atgaaagttt aactaccgtg
     5161 ttcagcgtgc tggtggtcag cagctttctt tatatctggt ttgccacgtt ctgatctttc
     5221 ttcagccgta cccaggcccg cgtgccggaa gtctcttgcc ggttttgcag gaaaaactgc
     5281 ccgtgatgca actgtgtaat gcggctgaca atacttaacc ccagaccaat cccgccataa
     5341 cggctgtcca tacgtacaaa cgctttactc aactccccgc atttactctc atcaatacct
     5401 ggtccttcat cttcaactgc catgaccgct ccgccatctt cttgcagctt aatcataatg
     5461 ttgctgcctt gcgggctgta acgatgggcg ttttctacca ggtttcgcaa taacatccgc
     5521 agcagggttg catcaccctg aacggtgatg tcggcggcgc tctctggcaa tagcagggtt
     5581 tgctgtcgct ggtcgagcat ggtactgagt tcgtcatacg aggggagaat gacatcttcc
     5641 agcagtttta catgttgata attaccggaa gaaaatgact gtccggcacg cgccagttgc
     5701 agcagctggg agacgctctc catcatctga tcaagccgtg ccactaacgg tgctacatca
     5761 atgtgatgcg ttttcgccag cagttccaga tgcaaacgca cccccgccag tggcgttcgc
     5821 agttcgtgcg cgacgtcagc ggtaaacaac ctttcgttat ccagcgtgct ggtcaggcga
     5881 ctgaccagat cgttcaacgc cgaaaccacc gcttcgattt cgagggtggc gctgtgaatg
     5941 gcaatgggcg ttaagttgtc ggcggtgcgc gcttccagct ctttttgcag ctccgccagc
     6001 gggcgggtga tgcggcgtac cgcctgatag cagataaata gcgtcaggct gaccataaag
     6061 acgccgggga caatcaggct ggcgaccgcc tcgcggatct cacgcatgat gtggcgatcg
     6121 ttgttgcgat tgtcgcgtag cgcctgctca aacagctgaa tctgctcggt actttcatgc
     6181 catagccaga agacgctgat cagctcaaac accaacaaaa tggccccgat ggtcaatatc
     6241 agccgttggc gcagcgatat aggtcggcgc agaaaacgca tcagattcaa ttagttttcc
     6301 tcattcgcga ccagcatata gccaaagccg cgcacggtgc ggatacgggc tttgcccact
     6361 ttgtcgcgca gattgtggat atgcacttcc agggtgttgg tcgagggttc attgtcccag
     6421 ttatagatgt cgttgtagag aatttcccga tgtaccggac tgcctgcttt gagcattaac
     6481 cgtgacagca gagcatattc tttgggcgtc agaatcaact cttcaccgcc catccatacc
     6541 tgacggcgac ccatattcag cgtcagattg ccaacaatca gctcactttc gccctgatta
     6601 ttatggcgtc gtagcagggc gcggatacgg gcgtgtaact cttccagcgc aaaaggcttc
     6661 accagatagt catcggcacc gacatccagc ccggcgattt tgtcggtcag cgtatcgcga
     6721 gcggtgagga tcagtaccgg cagggtgtat tttttctgcc ggatacgggc gagaaaatgc
     6781 agtccatctt cgtcgggtaa ccctaaatcc agtaccacca ggctgtaatg accgtcctca
     6841 aggctttgtt ccgccatccg cgcggttgtc acgccatcgc acgcgtagcc ttcggtttgc
     6901 gccgccagaa tcagtccctg caataacagc gtatcgtctt caacaatcag aattttcatt
     6961 tactcactct cctgcaagtt tgcagaatat catccgcggc ctggtaatac ttcgtctcaa
     7021 cgccagttaa tcccaatagc gtggagaata aattgtcttg tgaatagtgt tgcgtttgcg
     7081 cctgtttttg caggcagttc tggtcaacct gataccgctt ttgataatcc tccgacagcc
     7141 acagcagcat tggcacctgt ttttggctat ccggggcgat ggcataaggc agaccgtgca
     7201 gatagatgcc attttcacct aacgattcac cgtggtcaga aagataaacc aggctggtgg
     7261 taaatttatc ctgatgttct ttaagcagat taatcgcttt atcaacaata tagtcgacgt
     7321 aaaccagcgt gttgtcgtaa gtgttcacca gttgctcttt ggtacaggtc tggatctcat
     7381 tggtgtcgca ggttggggta aatttcctga actgcggcgg atagcggttg taataggtcg
     7441 gaccgtggct gccgatggtg tgtaagacaa tcacgccatc accctgcagg ttattgatgt
     7501 actcttccag cccgtggaac agcacttcgt catagcattc gccgttgatg cactgatcag
     7561 gcagattcag cgcggtgacg ttctggtgag gcacgcggtc gcaggcacct ttacagccgc
     7621 catcgttgtc attccacagc acgttgatgc ccgctcgctg aatgatatcc agcacgcctt
     7681 cctggtgctg tgccagctct tctttgtagt gctcacgcgg catatccgag aacatgcacg
     7741 gtactgaaac tgccgttgcc gtgccgcaag atgcggtatt agggaaatag accacgttat
     7801 ctttcgccag ccgcgggtta gtttcacgcg ggtagccgtt gagggagaag ttctccgccc
     7861 gcgaggtttc gccgacaatc aggatggtca aatttttacg tttttcgttc tgcattaacg
     7921 ggttgcggtg cgcgtcttca ccaattcgca ccagcggcag atttgccagt cgctgatggg
     7981 agtaccatga ccagctggca acaatgctgt tagaggggct taaggatttc accagctctt
     8041 tgttattgcg gaacaacgag gcgtagtctt tataaaacag tgcggcgacc agcaaaatca
     8101 gcagtacaga aaccagaata ttggctccac ggaaaagaac actgcgcaga cgcgaggtgg
     8161 caggtttgat ttttagccag caggcaatca gctcagcaag cacgccgctg aatcccagcg
     8221 ttaataacat ttgcggtgtc atcagcgcat aactttctgc tggagtggta tcaataatat
     8281 tggcaatcat cgagcggtcg atgacgatgc cgtaagtcat tatgaaatat tgtgcagccg
     8341 cgccaatcag aataaacagg caggccagtg gtcgattaag ccataagaaa gagcttagtg
     8401 tcaggacaat attaatcacg ctgaaagcga caaccggcat cgacaagaaa accagtacgt
     8461 tatgcagcga atccagcggc agcgcctgca acacctgttt aaaaaaggca atattcaggc
     8521 agatagagat ataaaaagcg gccaacaata gccaggcgag taaattcaaa gagggtcttt
     8581 ttagtaggcg cttcaacatc acggtgtttc catcgaacaa agtgcgcata tgctcgcaaa
     8641 gcaaaattaa gccaacctta agttcttaag gttggctttt atgtttgctg gattaagggg
     8701 aattaatctt tgcttattgg tgcatctaag ggatacgggt ttttatgtag ccggttgtaa
     8761 ttcagcgcat acatggcggt gatgaccatc agggtgacaa atgaccacat aacctctttc
     8821 gccccggacc ccaccacggc ccaaatgcag tagaggaagg caatggtagt aacagccaga
     8881 tatgccgggc gtgctttacc aaagtgaccg tggccgagca gcagtaacgc cgcacaggtg
     8941 taaagatatg gcaccagtgt aaagatgacc gagacggaag aaaccagacc gaactctttg
     9001 gtcgcgtttg gtgaaatgct gctgagctgg aagatggtca tcaaaatacc gacgataatc
     9061 agccccgcca ctggcgtacc cgctttattt acacgggcaa aaatcggtgg gaacagtccg
     9121 tcatcggcag cggctttcgc cgtttgaccc gccagcaacg tccagccgcc cagtgaacct
     9181 aagcaacccg cagctgcgca gaaggaaaca atggccccag cggtgtcacc caacgccatc
     9241 cgtgcggcat caccgaatgg cgaggcagaa acgcgcagtg cggcattagg gatcatcccc
     9301 ataatcgcgg tggtggagag cacatagcaa acggcggcaa tcaatacccc accaatggtg
     9361 gcgataggga cattgcgttt cgggtttttc accacacctg cggcaacgga ggcactttcc
     9421 acaccgatga acgaccacag cgtaacgtta agggtacttt gaatcgcacc gaaggtgccc
     9481 aggccgctga cgttccatgc cgccatatag gtttcaccac ggaaccagaa ccagccaaat
     9541 acggcaatcc cgacgatggg gatcagcgcc agcacagtgg caacggcctg cacgcgggtg
     9601 atcattttcg gaccgacaat gttcagcagg acgaagatcc acagcaccac gacgcaggtg
     9661 atggttaata ccaatggatc tttcagaatc gggaagaagt aacttaaata tcctacgcca
     9721 atgaccacca tggcgatatt gccgatccag caggccagcc agtagaggac gttggtttga
     9781 taaccgagaa acgggccaaa gcagcggcgg gcgtaagcgt aagaaccacc aggacttggg
     9841 tcgaggaacg acattttggc gtataccatc gagagcccca gcgcaccgat aatcgtcacc
     9901 aaccatccat aaatggcaat cccgccagtg gaggccaggt ttgcaggtaa cagaaaaaca
     9961 cctgacccca taatattccc cgacaccatc agggtgacgg ggattaagcc cactttgtga
    10021 gcatcagcat ccgaagacat aattaaactc ctgcgaaggc gagcttcgtg acaataaatt
    10081 acgtcatatc atacgcctgc attcgatagt tgaattatta tcgaccgggt tatcgctgat
    10141 gccgttatta atatagctct tcgttaaggt taaaaatcag gcgacagtgc gttctctgtg
    10201 ctgactaaca taatgcagcg gcgtcatacc gtagaagtct ttaaagacag aaataaagta
    10261 cgacgtactg ttgtagccgc aggactgtga tacctgagag atatttttac cgtccatcat
    10321 taattcattt acggcataac gcatgcggca ggtggtgatt atttggctat aactggtgtt
    10381 ttcgcttttc aactttttct ttaacagact tgggctaaga cataaacaac tggctaccat
    10441 actaagattc cagtctttgt gaatatcgct ttcaataatt tgataaacgc tgtcacttac
    10501 acaattacgt aacatataca tcattaatga aaggaatttt ttactgtcga gaaaacggga
    10561 tagcaccgtg aataataatg cgcgtgttcg ttccttttct gactcagttt ctgcgggcat
    10621 catgctgtgt tgcgcggcaa cgcgaaagac gtctggcgtc aggcatggca gggtgagtat
    10681 gggcgtagcg ctacgttgcc atactggtat ttgcgagaga tctttattca gaaaccggag
    10741 gtaatctttg atgatgtcgt gactaatatg agcaaccaaa gtgttgttaa gagaggagac
    10801 gtcgataata ttattttcac aatttaataa cgccatatgg ttagctttaa ggctaatagg
    10861 ttctttccca ttcaccctta tccagacatc tttttcagtc aataaaacaa tacaaggttg
    10921 gtcgctgcaa atcctcatac atgtactcct gagtgcgaat aaaagaccag ttctcgctaa
    10981 agcaaagcga taccgggaaa tggttatata attgcatgat gaatataaac atgcataaaa
    11041 ataacgagtt ttactaatat tacaaatttt tatagtaatt tctcatgtac atattttttt
    11101 acaggataaa taacaggcaa aaaaaagcgt ggggtggggc cgcgcaaaaa gtatgacatt
    11161 tttgtttgca gcaattttta tgaaaacgtt gccatactca aaaggcctaa ggccaggcgg
    11221 cgtaatgtta tttaaacaat tacgccttca gcggaatagt ggttacgctt tcacgcacat
    11281 aacgtggtaa ataccgtcaa taatttcagt cccttcagtt tcgtgttcaa atctagggaa
    11341 atggtggtcc caggattgca gcgagcgtaa ataacttact tgcggactgt ttttatcgcc
    11401 gaagttttca ccagacagca gcatcgggat tcctggcgga tacgggataa ctgagtttgc
    11461 cgcgatgcgt cctggcagat tttcaatgga taccagttcg acattgttgt cgacaatcgc
    11521 gttgtacgct tcacgcgggg tgatttccgc caccggcagg ccggaatagg cttcgttcaa
    11581 ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat
    11641 ccccatgttc gcgtaagtgt caggatattg ttcaacaagt tccggcatca cctgcgccag
    11701 cggtgtgttg gcgtcatagt ggtgtttgaa ggagcaaagg gtgttaacca gagtccccca
    11761 tttcccacgg gttacgccca tagagaacag gaacataatt tggaagtcag tggtgcgggt
    11821 aggcacaatg ccgtggcgac caagccaggc agtgaccagc gccgccggaa caccggtttc
    11881 ttccagttca ccatcttcac ccattcccgg agcaaggatg ctgactttaa ttgggtcgag
    11941 catactccag ttatccggaa tatctttgaa gccgtgccag ctttcgcccg gatgcattac
    12001 ccagcagtcc tgaacggtgg tcagcagttt ggttggtgcg tcagcaaagt cataggtttt
    12061 gccggtttgt gggtcggtga cgacttcttt gttccacggt ttgaagaacc agctaccgtc
    12121 agcggtgaac tctttatata gccgcgccat cgcctgacgg aaatcaaccg cttcgtcaat
    12181 cacttcctgt gtcagtgaca ggccgctgtt gccgtccatc atcgacaccg ccacgtcgtt
    12241 ggatgcgcag atggcataca gcggggaggt ggtggcatgc atcatgtagg cctggttgaa
    12301 gcgggagaag ttaatcgccc cacgaccttc acgtacatga atataagaag cctgtgacag
    12361 cgcattcagc agtttgtgga tggagtgggt ggcgaaaacg gtaggaccgt tgtgatcgcc
    12421 aggttcgccg cgcatggcat agtgatcggc atagatcggg ttgaaacgtg catagccgta
    12481 ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc agcagatcct gcgcttcttt
    12541 agcgttataa cacacgccgt cataggtgca gttggttacc acgcagtaag acggtttttg
    12601 cccggctttg tctttggtca gcgggctttc actgattttc ttttgcaagg tttcaggttg
    12661 catttcctgc ggatagattg gcccgataat gccgtagcgg ttgcggcttg gcaccatata
    12721 gaccggtttc gcgcctgtca gcatcaaacc ttgttcgatg gatttatggc agttacggtc
    12781 aacgaccacg acatcgttat cggtcatgca agcctgcatg atggtgcggt tagagccgga
    12841 agtaccgacg actaccgacc aggagcgatc ggcaccaaat acgcgtgcgg catatttttc
    12901 gctttcgcca aatgcgccag tatggtcaag caaagaaccg agggaagttc gttcgatgcc
    12961 catgtcggta cggaacagat tttcaccata gtagtcatgg tagaaacgtc cggcgggtgt
    13021 tttggtaaaa ccaacgccgc cctggtggcc tggcgctgcc caggaatatt catggatgtc
    13081 actatatttc atcagcgcgc tgaacagtgg cggcaacagc tgctggcggt agcgggtcat
    13141 cgcggcaacg gcgcgtccgg cgataaagtc ggcggtatct tccagaatcc aggcgaattc
    13201 atcgacaagc tccagcagat cccggtccat tgcggcgagg gctttttccc gatcgcccaa
    13261 caggaagacc ggcacgtttt gttggcgctc atgaagctta ccgatcaatt gtctgacgtt
    13321 ttgatgttcg tccggatgct ccatttgata gctgaacatc aggcagtcaa tggcttcgtt
    13381 tgaagagaga atggcaaaac catcatcaaa ggaggtggat ttaatcacgg taacattttg
    13441 ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg acccaggtgt cttgatggag
    13501 aaactcgctt tcaacaatta atactttcat cattgcttac ccggttatga aggaatcttc
    13561 gttgcgggca agtatcttcc ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt
    13621 ttgaaatatt tagttttata aacaatacgt tatgtgatta ttttaaatta ttttcactgc
    13681 ttattatcga cggctaaact atttttttgg ctgatactga tatcgtcttt agccgggaaa
    13741 cgtctggcgg cgctgttggc taagtttgcg gtattgttgc ggcgacatgc cgacatattt
    13801 gccgaacgtg ctgtaaaaac gactacttga acgaaagcct gccgtcaggg caatatcgag
    13861 aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat
    13921 gtactgtttc atcgtcaatt gcatgacccg ctggaatatc cccattgcat agttggcgtt
    13981 aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt tttcggcaat
    14041 aaagcccagc atctggctaa cataaaattg cgcatggcgc gagacgctgt ttttgtgtgt
    14101 gcgcgaggtt ttattgacca gaatcggttc ccagctagag aggctaaatc gcttgagcat
    14161 caggccaatt tcatcaatgg cgagctggcg aatttgctca ttcggactgt ttaattcctg
    14221 ctgccagcgg cgtacttcaa acgggctaag ttgctgtgtg gcaagtgatt tgatcaccat
    14281 gccgtgagtg acgtggttaa tcaggtcttt atccagcggc caggagagaa acagatgcat
    14341 cggcagatta aaaatcgcca tgctctgaca ggttccggta tctgttagtt ggtgcggtgt
    14401 acaggcccag aacagcgtga tatgaccctg attgatattc actttttcat tgttgatcag
    14461 gtattccaca tcgccatcga aaggcacatt cacttcgacc tgaccatgcc agtggctggt
    14521 gggcatgata tgcggtgcgc gaaactcaat ctccatccgc tggtattccg aatacagcga
    14581 cagcgggctg cgggtctgtt tttcgtcgct gctgcacata aacgtatctg tattcatgga
    14641 tggctctctt tcctagaata tcagaattat ggcaggagtg agggaggatg actgcgagtg
    14701 ggagcacggt tttcaccctc ttcccagagg ggcgagggga ctgtccgagc atcatgaggc
    14761 cgaaaactct acttttcagg taatttattc ccataaactc agatttactg ctgcttcacg
    14821 caggatctga gtttatggga atgctcaacc tggaagccgg aggttttctg cagattcgcc
    14881 tgccatgatg aagttattca agcaagccag gagatctgca tgatgtctgc acccaaaatt
    14941 acatttatcg gcgctggttc gacgattttc gttaaaaata ttcttggtga tgtgttccat
    15001 cgcgaggcgc tgaaaacggc gcatattgcc ctgatggaca ttgaccccac ccgcctggaa
    15061 gagtcgcata ttgtggtgcg taagctgatg gattcagcag gggccagcgg caaaatcacc
    15121 tgccacaccc aacagaaaga agccttagag gatgccgatt ttgtcgtggt ggcatttcag
    15181 attggcggtt atgaaccttg cacggtgact gatttcgagg tctgtaagcg gcatggtctg
    15241 gaacaaacca ttgccgatac gttggggccg ggcggtatta tgcgcgcgct acgtaccatt
    15301 ccgcatctgt ggcaaatttg cgagaacatg acggaagtct gccccgatgc caccatgctc
    15361 aactatgtta acccaatggc gatgaatacc tgggcgatgt atgcccgcta tccgtatatc
    15421 aaacaggtcg ggctgtgcca ttcggtgcag ggaacggcgg aagagctggc gcgtgacctc
    15481 aatatcgacc cggctacgct gcgttaccgt tgcgcaggta tcaaccatat ggcgttttac
    15541 ctggagctgg agcgcaaaac cgccgacggc agttacgtga atctctaccc ggaactgctg
    15601 gcggcttatg aagcagggca ggcaccgaag ccaaatattc atggtaatac tcgctgccag
    15661 aatattgtgc gctacgaaat gttcaaaaag ctgggctact tcgtcacgga atcatcagaa
    15721 cattttgccg aatatacgcc gtggtttatt aagctaggtc gtgaggattt gattgagcgt
    15781 tataaagtac cgctggatga gtacccgaaa cgctgcgtcg agcagctggc gaactggcat
    15841 aaagagctgg aggagtataa aaacgcctcc cggattgata ttaaaccgtc acgggaatat
    15901 gccagcacaa tcatgaacgc tatctggact ggcgagccga gtgtgattta cggcaacgtc
    15961 cgtaacgatg gtttgattga taacctgcca caaggatgtt gcgtggaagt agcctgtctg
    16021 gttgatgcta atggcattca gccgaccaaa gtcggtacgc taccttcgca tctggccgcc
    16081 ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg aagctattct tacggaaaat
    16141 cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata ctgccgccgt gctgggcatt
    16201 gacgaaatat atgctcttgt tgacgacctg attgccgccc acggcgactg gctgccaagc
    16261 tggttgcacc gttaaaacgc gactaaacgc tactgcgccg ggggatttat tccggcgcac
    16321 acctctgacg ataccaataa cagaaggcgg gcgttggtaa cagcgacccg ataccctatg
    16381 agcatttcaa tgactacaaa actcagttat ggatttggag cgttcgggaa ggattttgcg
    16441 atcggcattg tgtatatgta cctcatgtat tactacaccg atgtcgtcgg gctgtctgtg
    16501 ggtttggtcg gtactttgtt tctggtggcg aggatctggg atgctattaa cgatccgatt
    16561 atgggatgga ttgtaaatgc tacgcgatcg cgatggggta agttcaaacc ctggatcctg
    16621 atcggtacgt tggcaaactc tgtaatctta tttctcctct ttagtgcgca tctgtttgaa
    16681 ggtactactc agattgtctt tgtttgcgtg acctacatcc tctggggcat gacttacacc
    16741 attatggata ttcccttctg gtcgctggtt ccaaccatca cgctcgataa acgtgaacgc
    16801 gaacaactgg ttccttatcc gcgttttttt gccagtctgg caggctttgt tacggcaggt
    16861 gtgacgctac catttgttaa ttatgtcggc ggtggcgatc ggggatttgg ctttcagatg
    16921 ttcactctgg tattgatcgc cttttttatt gtttcaacca tcatcactct gcgcaatgtg
    16981 catgaagtct tttcgtcaga caatcaatcg tctgctgaag gaagccatct gacacttaaa
    17041 gccatcgttg cgcttattta taaaaacgat cagctttcat gcctcttggg tatggctctt
    17101 gcttataatg tagccagcaa cattattacc ggatttgcta tctattattt ctcatatgtt
    17161 atcggtgatg cggatttgtt cccctattat ctgtcgtatg cgggagctgc taacctggtg
    17221 acgttagtat tcttcccacg cttagttaaa tcattatccc gacgcatttt atgggccgga
    17281 gcatctattc ttccggtgtt aagctgtggt gttctcctgt taatggcatt aatgagctat
    17341 cacaacgtcg tcctcattgt gattgcgggt attttgctga atgtgggaac ggcgcttttc
    17401 tgggtattac aggtcatcat ggtggcagat accgttgatt acggtgaata taaactgcac
    17461 gtacgctgtg aaagcatcgc ttactccgtg cagactatgg tggtgaaggg cggttcagcc
    17521 tttgcggctt ttttcattgc ggttgtgtta gggatgattg gctatgtacc gaatgttgaa
    17581 cagtctacgc aagccctatt aggtatgcag tttattatga ttgctctacc gactctgttt
    17641 ttcatggtaa cgctgattct ctacttccgt ttctatcgcc tcaatggcga cacgctgcgc
    17701 aggatccaga ttcatctgct ggataaatat cgcaaagtac cgcccgagcc tgttcatgct
    17761 gatattccgg tcggtgcagt gagtgatgtg aaagcctgac gtggacaata gctaacgaaa
    17821 atggcctgat gcgatatgtt tatcaggcca cgtcttaatg taatgctttg gatttgcaaa
    17881 ttttgtagac cggataagga attcacgccg catccggcat caacaaagtg cacgttgtta
    17941 tccagttatc aagccatagc gccgtagctg gcggcaatga aagcgagcga gcaatatcac
    18001 tgtcgttaat gcgccaagca gcgcgcagaa catatcggat tgcgtgtccc actggtcgcc
    18061 ctgggtaccg agaaaatcat ccgctccctg gcccatcgcc agtgccgccc accactcgat
    18121 taattcatac atcgcgctta tcgccagcgc cacgcaacag accagaaacg ccaccatttt
    18181 acgtccgcgc acgtacatcc cgcgaacgag aatttctcgt gccaccagtg caggcaccag
    18241 cccctggaaa aagtgcccca gcttgtcata cggattacga ctcaacccca gccattcctg
    18301 cacctcaaaa ccaacgggga ctttcgcgta ggtgtactgc ccgcctacca tcaggatgat
    18361 ggcgtgaaag aagatgagcg tatagagcag cagcgttaac gggtaacgtc tggcggtggc
    18421 aagtagtagc ggcacgacga taatcaccgg tgtcacttcc agcagccagg tgagtttgtc
    18481 atgggccgaa atgccggtat aaatcaggat taacgttagc gccagtgcgc cagtgttaag
    18541 aattaacggc ttgagtgtgc gggtcatggt gagttcgcaa gtcagggaaa atactgacta
    18601 ttcaccagca agcatgaaat tacaatcgca aaagaggtgg cccaggggga tcacctggca
    18661 gcatgctgcc aggcgctggg ccgaagaggt tacttagtgc agttcgcgca ctgtttgttg
    18721 acgatttgct ggaagaagtc gttacctttg tcatcgacca ggataaacgc cgggaaatct
    18781 tctacttcga ttttccagat agcttccata cccagctccg gataagcgac gcattccaga
    18841 tgcttgatgc tctgctgcgc cagtaccgcc gccggaccgc cgatgctacc gaggtagaag
    18901 ccgccgtgtt tatgacacgc gtcggtaacc tgctggctgc ggttgccttt cgccagcatg
    18961 atcatgctgc cgccgtggga ttgcagcaga tccacatagg agtccatacg acctgcggtg
    19021 gttgggccaa gtgaacctga tggataaccg gcaggggttt tcgccggacc cgcgtagtag
    19081 atcgggtgat ctttgatgta ctgcggaagt tctttaccgg cgtcaatcag ctctttcagc
    19141 ttggcgtgtg caatatctcg acccacaata atggtgccag tgagcgacaa acgagtggaa
    19201 accggatatt gcgaaagctg ggcgaggatc tctttcatcg ggcggttaag gtcgaccttc
    19261 accgcttcgc cttcaccggc ctgacgcagt tcttgtggaa tgtactgacc tagattgtgt
    19321 tccagttttt cgatccagat accttcgcgg ttgattttcg ctttaatgtt acggtcagcg
    19381 gagcaggaga cgcccatgcc taccgggcag gatgcgccgt gacgtggcag acggataacg
    19441 cgaatgtcgt gcgcgaagta tttaccgcca aactgcgcgc caagaccgag tttctgggcc
    19501 tcttccagca gttcctgttc cagctggaca tcgcggaacg cctgaccatg ttcgttccct
    19561 tccgtcggca gttcatcgta atagtgagcg cttgctaatt tgacggtttt caggttgttt
    19621 tccgcagacg taccgccaat cacaaacgcg atatggtacg gcgggcaggc tgcagtaccg
    19681 agggtacgca ttttctcgac gaggaagttt ttcagtttgc cgggagtcag cagggctttg
    19741 gtttcctggt agagatacgt tttgttggca gagccgccgc ctttcgcaac gcaaaggaat
    19801 ttgtactcat cgccatctac cgcgtacagg tcgatttgcg caggcaggtt agtgccggtg
    19861 ttgacctctt tgtacatgtc cagcgccgcg ttttgcgagt agcgcaggtt atcttcgata
    19921 taggtgttat agacgccttt cgacagcgct tcttcatcac cgccaccggt ccacacgcgc
    19981 tggccttttt taccgacgat gatcgcggtg ccggtatcct ggcaggtcgg cagcacgcct
    20041 ttggcggcga tttcggagtt tcttaagaat tgcagtgcca cgtacttgtc gttttcgctg
    20101 gcttctggat cgtgaagaat agccgcaacc tgtttctggt gtgccggacg tagcatgaaa
    20161 gaagcgtcgt gaaaggcttg ctgcgccagc agggtcaggg cttctggttc cactttcagg
    20221 atggtttcgc cgtcgaagtc ggcaacgcta acgtaatcgg aagtgagtag atagtattcg
    20281 gtattgtctt tccccatcgg gaaaggtgcc tggtagataa agggtttgtt tgacatagct
    20341 tccagcctgt aactctgtat ttgttattcg aaaatggcgt gccgcgtagt cacgggcggc
    20401 acgcaaagtg catttataag aacccgtaca tcgcggcgaa gatccagccg aagacgcacg
    20461 atacgctcac accaatcaac cccggcagaa taaaactgtg gttgatgacg aagcgaccga
    20521 tgtgggtggt gccggaacgg tcaaactgaa tcgctgccag atcgctcgga taagtcggca
    20581 ggatgtaata accgtagcaa gccggtgctg aagccacgat gtatgccgga tcaacgccga
    20641 tcgccagcgc gaccggaaca atcgccgcca gcgccgcagc ctgagagttt acaaacttgg
    20701 aaaccagcag cagaacaatg gcatacgccc acggatactc tttcaccatt tcacccagta
    20761 cgccctgaat ttcagacata tgcgcaccga acatggtttc tgccatccat gcgataccgt
    20821 acaccgccac gatggcgatc ataccggaac ggaagacttc gttttttgag atagacgcgg
    20881 gattggtttt ggtcaggata ataatcagcg ccccggtcag cagcataaac atctgaataa
    20941 ccagtaccat cgacagcggt ttgccgccga aggatggacg caggtccgaa tcagcaccaa
    21001 gaagggcgac tacagcgatt gccccgagga aaatccacat tgccagccag ttgcttttcg
    21061 gcagtttttt atccagcagc gtcgcggtat caccgtaaac atactcacgg ttttccggta
    21121 cggagatgaa tttctggaac tcttcgtctt tatccagatc tttaccgcgg aaccagctga
    21181 agataccgat cgccaggata ccgattaacg tcgatggaat ggtgattgcc agcagatcga
    21241 ggaactcaag atggcgacca tcaaaggtga cattacccag catcgcaacc agagacacga
    21301 ccgcaaccga caccggactg gcgataatcc ccatctgtgc accgatagaa cttgccgcca
    21361 tcggacgttc cggacggatg ttgttcttaa tggcgacgtc gtagatgatc ggcagaatgg
    21421 tgtaaaccac atgacccgta ccgcaaagaa tggtcagtgt acaggtcaca aacggcgcga
    21481 caattgagac atatttcggg ttgcggcgca gcagcttctc ggcaatttgc agcatgacat
    21541 caagaccgcc cgaagcttgc aaggtcgccg atgccgccac caccgcaatg ataaccagca
    21601 tgacatcaac tggtggttta cctggctgaa ggtggaagac gaagaccaga atgaccagac
    21661 cgataccgcc taataaaccc agcgcgatac caccctttct ggcaccataa aacagacata
    21721 ttagtattat gataagttgg atagtaaata acatgtgtga accctcgcga taatcctatt
    21781 taaatttttg ctgaatagat cacagtcacg ttctgttttg tatgaactgt tttcagagcc
    21841 tgactaaata tccgtttggt cgttaccggc tttagcaaat acctcacagt gaatattggc
    21901 tgattaaagc agcggcaaat ttatgcactg tgataagcgg ctttttcaga gagaaaaact
    21961 ctctggcgat gggtttatag tttgtactgc gcagatgtta atgttttgtt tgctgttatc
    22021 ttattgatat ttatcggtct attttttgtt ctcagggcag atatcattag gtttatagat
    22081 taatctgatc tacccatttg tgggtaaaaa tacacataat gcgggtgaca taatagttaa
    22141 ttaacttttg ttatcgtttt gaaattaaaa acaccgttca tctgaagaga tattaatttt
    22201 taacgatgat ggagggataa ttatatttga tctggcacaa gttttactga ttaacgatgt
    22261 aacttgtgcc aggggtattt tgcattacgg taataattat tacttacaga taaccagcga
    22321 tccgttattg gcaatattgt ttcagcagtg agtagtgttc tgcctgaata cggtaacggt
    22381 aaaccggacg ccccgtgacg ccataatgga tactggtgaa caagatgtgg cagttgacca
    22441 gccagatgag gtattttcgg caggaaacac gcgagatgtt aacctcgtta gccagctcgt
    22501 cggttgaaaa ctcatagtcc tgatgcgcgt caatccactg gcacagcgtg cgtaacgtct
    22561 gcggcgttaa gccttttggc aagcgacgag gatcctgttc gttggagctg ctgccgtgga
    22621 ttagctgatc aagctcggcc tggtcataat actgatgttt ttccagcgcc attttctttt
    22681 gccgccagcc ggtgagcgcc tcttcaaagc gggaagcctg gaagggtttg atcaggtaat
    22741 ccacgacacc gtaatgcagc gaatctttaa tggttgccgc atcggctgcg gaggagatga
    22801 caatcacatc acttttgcaa cgcgcgttat gcaggacagg cagtaaatcg agcccgttct
    22861 ctttttgcat atagatatcg agcaatatca ggtcgatagg cgcatcgcta ttgaagataa
    22921 tctctttggc tttctccagc gtcgaggctg ttccacagca ttgaaagcct gggatttgtg
    22981 ctacgtatcg gcgattcagc tccgcgacca ttgcgtcgtc atcgataatt aatacattga
    23041 tcatctgttc gacctctccc cgtcccaggg tatctggaca aaaaattgtg tgaaaatccc
    23101 cggttccgat tccacggcga tgctgccgcc gagattttct acctgttgtt tgacaagtgc
    23161 taaaccgacg cctcgctcgc ttccttttgt cgagacacct ttgtcaaaaa tgtgatcgat
    23221 tttgtcgggt gcgatccccg gtccatcatc gttaacttcg cagtgcagcc agccgtgacg
    23281 gtagtgcaat gttacgctaa tttcgccacc gggttccggc cctaatgctt ccagcgcgtt
    23341 ttctatcaga tttcccaatg tggtaatcag cgtcgcgacc tggtcctcac tgccgctgtc
    23401 tggcagctgg ctttcactgt ttaaaatcag cgtatggcct aaatcggtcg cgcggttaat
    23461 cttgctgatt aaaaaaccag cgataaccgg agatttgatc ttacccagca gagagccaat
    23521 ctcttcctga tagttattgg ctgttttgag aatgtaatct tccaactgct tataactctt
    23581 cagatgcaat aatccgagaa tcacatgcaa tttattcata aattcgtggg atcgttcacg
    23641 aagtgcgtca gcatagttga ccagaccgtc gagtcgctgc atcagcttac gaacttcagt
    23701 tttgtccctg aaggttgaaa tggcaccgat gataacgcca ttactgcgca ccggaacggt
    23761 gttgatcagt aatagccggt ctttaatcgt aatctcttcg tcgcggcgcg gcgtaccgtc
    23821 gcgtaacact tccgagacat ctaccacctg tgaccatgag tggctgagcg tcgacagttt
    23881 ctcatcgtcc tgcgacttac ggtaattcag caattcttgt gcggcatcgt tgatcagcgt
    23941 gacctcgccg cgatcgtcca cggcaacgac gccttctttg atagactgca acatggcctg
    24001 gcgttgctca aacagcgtgg agatttcgta gggttccagg ccgaaaagga tttttttcag
    24061 taccttaacc agaatgcagg tgccaatcag tccgaccagc atgccaaata ataccgacca
    24121 gataatgctc cagcgactgt cattgatctg ttgggtcacg cggcttaact caaggccgat
    24181 cgccaccacg ccaatttgtt tatgattttc atcgtagatg ggggtaaata cgcgtaaagc
    24241 ctgcgccaga aaaccgcgat tgatagcgac attttcttcg ccattcagcg cattaaggat
    24301 gtcatcacct ttaaatggct gaccaatacg ctgggcttca ggatgtgagt agcgaagact
    24361 ttgcatatcg gtaacgacaa taaacagcag atcgttgcgt ttgcgcacgg cttccgcgat
    24421 ggcctggatg ccactctcct gcggtttttt ctgcaagccc tgacggattt ccggcgagtc
    24481 ggcgagggta cgcgccactg ccagtgcctt gttggccagc ccgtctcgcg tcatatcact
    24541 gatttgcgag aagtaaatca gatgcaccac caatagcacc gagaacagta ccgcgctgac
    24601 cattaagatc acagtggtac tcaatttcat cggacgtttg cgtaatatgt ggtagggcaa
    24661 tgaatgtctc atcagcttcc ttgtgtgaca aatttcttaa gcattatctc tgatgaggcg
    24721 ggtaattcaa agggagtaaa aatgattggc tatatagggg aagagactct ggcaacggaa
    24781 actgccagtg ctgtatgaag attccggggc tatgcttata gcgataatca tactgatgag
    24841 agagggaagg tcatggatca ggcgctactg gacgggggtt atcgctgtta taccggcgaa
    24901 aagatcgatg tctatttcaa cactgcgata tgtcagcatt ctggcaattg cgtacgtggc
    24961 aacggcaagt tatttaatct caaacgaaag tcgtgtagac tggccccctg aatctccaga
    25021 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact agtcattgga
    25081 g
//
LOCUS       DAZPDE010000044        24654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPDE010000044 DAZPDE010000000
VERSION     DAZPDE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.044"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHU0J_001655"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHU0J_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHU0J_001656"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHU0J_001656"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1216387.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHU0J_001657"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHU0J_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1216388.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHU0J_001658"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHU0J_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1216389.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHU0J_001659"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHU0J_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1216390.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHU0J_001660"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHU0J_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1216391.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHU0J_001661"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHU0J_001661"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1216392.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHU0J_001662"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHU0J_001662"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1216393.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHU0J_001663"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHU0J_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1216394.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHU0J_001664"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHU0J_001664"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1216395.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHU0J_001665"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHU0J_001665"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1216396.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHU0J_001666"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHU0J_001666"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1216397.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHU0J_001667"
     CDS             10748..11212
                     /locus_tag="ACHU0J_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1216398.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHU0J_001668"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHU0J_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1216399.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHU0J_001669"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHU0J_001669"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1216400.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHU0J_001670"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHU0J_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1216401.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHU0J_001671"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHU0J_001671"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1216402.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHU0J_001672"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHU0J_001672"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1216403.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHU0J_001673"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHU0J_001673"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1216404.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHU0J_001674"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHU0J_001674"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR1216405.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHU0J_001675"
     CDS             complement(20505..20798)
                     /locus_tag="ACHU0J_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1216406.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHU0J_001676"
     CDS             complement(20795..22084)
                     /locus_tag="ACHU0J_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1216407.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHU0J_001677"
     CDS             complement(22140..23078)
                     /locus_tag="ACHU0J_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1216408.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHU0J_001678"
     CDS             complement(23098..23862)
                     /locus_tag="ACHU0J_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1216409.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24182..>24654
                     /locus_tag="ACHU0J_001679"
     CDS             24182..>24654
                     /locus_tag="ACHU0J_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR1216410.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5950 c         6485 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggggcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    24001 gcgattgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    24061 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    24121 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    24181 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24241 acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca
    24301 tgacaatgaa accgagctga tttacgtcaa gaaaggggtt gcaaggttta ccatcgattc
    24361 ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca ggctgcatgc
    24421 ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca
    24481 gtttcagggg gctgaggaaa atcagttact gcaaccgcat tcttgtccgg taattgccgc
    24541 ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga ttttgccgca
    24601 aagtaaaaat agccaaacat cttcgttatg ggatgcatta gcctatacat tagc
//
LOCUS       DAZPDE010000045        24646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPDE010000045 DAZPDE010000000
VERSION     DAZPDE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.045"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHU0J_001680"
     CDS             complement(<1..784)
                     /locus_tag="ACHU0J_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1216411.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIRLIILFLLAA
                     LSFIGLFFMINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNT
                     EEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGR
                     VIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKI
                     HVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLI"
     gene            complement(884..1795)
                     /gene="gsiD"
                     /locus_tag="ACHU0J_001681"
     CDS             complement(884..1795)
                     /gene="gsiD"
                     /locus_tag="ACHU0J_001681"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1216412.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1798..2718)
                     /gene="gsiC"
                     /locus_tag="ACHU0J_001682"
     CDS             complement(1798..2718)
                     /gene="gsiC"
                     /locus_tag="ACHU0J_001682"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1216413.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2736..4274)
                     /gene="gsiB"
                     /locus_tag="ACHU0J_001683"
     CDS             complement(2736..4274)
                     /gene="gsiB"
                     /locus_tag="ACHU0J_001683"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1216414.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4294..6165)
                     /gene="gsiA"
                     /locus_tag="ACHU0J_001684"
     CDS             complement(4294..6165)
                     /gene="gsiA"
                     /locus_tag="ACHU0J_001684"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1216415.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6152..7117)
                     /gene="iaaA"
                     /locus_tag="ACHU0J_001685"
     CDS             complement(6152..7117)
                     /gene="iaaA"
                     /locus_tag="ACHU0J_001685"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1216416.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7321..8556
                     /gene="moeA"
                     /locus_tag="ACHU0J_001686"
     CDS             7321..8556
                     /gene="moeA"
                     /locus_tag="ACHU0J_001686"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1216417.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            8556..9305
                     /gene="moeB"
                     /locus_tag="ACHU0J_001687"
     CDS             8556..9305
                     /gene="moeB"
                     /locus_tag="ACHU0J_001687"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1216418.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9484..10146)
                     /gene="fsa"
                     /locus_tag="ACHU0J_001688"
     CDS             complement(9484..10146)
                     /gene="fsa"
                     /locus_tag="ACHU0J_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1216419.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            10277..11176
                     /locus_tag="ACHU0J_001689"
     CDS             10277..11176
                     /locus_tag="ACHU0J_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1216420.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11182..13614
                     /locus_tag="ACHU0J_001690"
     CDS             11182..13614
                     /locus_tag="ACHU0J_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1216421.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13760..14575
                     /gene="ybiV"
                     /locus_tag="ACHU0J_001691"
     CDS             13760..14575
                     /gene="ybiV"
                     /locus_tag="ACHU0J_001691"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1216422.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14727..15992
                     /locus_tag="ACHU0J_001692"
     CDS             14727..15992
                     /locus_tag="ACHU0J_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1216423.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16233..17825)
                     /locus_tag="ACHU0J_001693"
     CDS             complement(16233..17825)
                     /locus_tag="ACHU0J_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1216424.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18044..18964
                     /gene="ldtB"
                     /locus_tag="ACHU0J_001694"
     CDS             18044..18964
                     /gene="ldtB"
                     /locus_tag="ACHU0J_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1216425.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19022..20140)
                     /locus_tag="ACHU0J_001695"
     CDS             complement(19022..20140)
                     /locus_tag="ACHU0J_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1216426.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20137..20604)
                     /gene="mntR"
                     /locus_tag="ACHU0J_001696"
     CDS             complement(20137..20604)
                     /gene="mntR"
                     /locus_tag="ACHU0J_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1216427.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20790..20918
                     /gene="mntS"
                     /locus_tag="ACHU0J_001697"
     CDS             20790..20918
                     /gene="mntS"
                     /locus_tag="ACHU0J_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1216428.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21190..22773
                     /gene="opgE"
                     /locus_tag="ACHU0J_001698"
     CDS             21190..22773
                     /gene="opgE"
                     /locus_tag="ACHU0J_001698"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1216429.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22821..23336)
                     /gene="ompX"
                     /locus_tag="ACHU0J_001699"
     CDS             complement(22821..23336)
                     /gene="ompX"
                     /locus_tag="ACHU0J_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1216430.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23389..23454)
                     /locus_tag="ACHU0J_001700"
     CDS             complement(23389..23454)
                     /locus_tag="ACHU0J_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216431.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23689..24576
                     /gene="rhtA"
                     /locus_tag="ACHU0J_001701"
     CDS             23689..24576
                     /gene="rhtA"
                     /locus_tag="ACHU0J_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1216432.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         6042 a         6676 c         6095 g         5833 t
ORIGIN      
        1 taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct tgtttcaaaa
       61 atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac cgccagttta
      121 aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg atacgtagcg
      181 taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga ttccagctgt
      241 tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa tcggcaaaaa
      301 taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga taaacaacga
      361 ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt accagtccca
      421 ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga atctcctctg
      481 tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca atatctttaa
      541 agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt tttaccgcgc
      601 gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac agtgctgcca
      661 ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta atacatttaa
      721 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
      781 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
      841 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
      901 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
      961 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
     1021 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
     1081 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagaagaga
     1141 cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
     1201 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa
     1261 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
     1321 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
     1381 gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc
     1441 ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
     1501 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
     1561 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
     1621 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
     1681 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt
     1741 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
     1801 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
     1861 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
     1921 tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
     1981 agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
     2041 gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
     2101 acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc gcgccatcac
     2161 ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
     2221 tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa acatccccag
     2281 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
     2341 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
     2401 catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct catcggcaac
     2461 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
     2521 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
     2581 ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatgtggac
     2641 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt
     2701 gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
     2761 ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
     2821 agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc ctgcgccgcc
     2881 ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc caggaagtca
     2941 tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca gttttgcgag
     3001 gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca gccagtgtag
     3061 aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
     3121 gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
     3181 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
     3241 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
     3301 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg agttgcatag
     3361 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
     3421 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
     3481 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
     3541 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
     3601 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
     3661 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
     3721 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt cggtgaaatc
     3781 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta
     3841 atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag
     3901 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg
     3961 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt gtaagtaatg
     4021 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
     4081 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcattcgcg
     4141 tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg
     4201 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
     4261 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
     4321 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
     4381 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
     4441 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
     4501 cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga gatacatcac
     4561 cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa acagatacgc
     4621 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
     4681 atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
     4741 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
     4801 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc
     4861 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
     4921 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
     4981 tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc cgccctgcga
     5041 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
     5101 cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc
     5161 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
     5221 ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt cctgtttcgc
     5281 tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt tcatcgcacc
     5341 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
     5401 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
     5461 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
     5521 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
     5581 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
     5641 ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt gtgcctccgg
     5701 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
     5761 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg ggttcagcga
     5821 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc gcatttgtgc
     5881 agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca gcattttatc
     5941 gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg cagtcactga
     6001 cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca gactaaaaga
     6061 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat taagattttc
     6121 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
     6181 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
     6241 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
     6301 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
     6361 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
     6421 ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
     6481 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
     6541 ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
     6601 catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc gctgccagta
     6661 gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc atgccatgag
     6721 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
     6781 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
     6841 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
     6901 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
     6961 ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca acaatggcag
     7021 acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
     7081 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
     7141 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat
     7201 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
     7261 tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
     7321 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
     7381 gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg tcgtattctg
     7441 gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc tatggacggc
     7501 tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc
     7561 tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc
     7621 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
     7681 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
     7741 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag
     7801 ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
     7861 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
     7921 atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta
     7981 atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc
     8041 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
     8101 accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
     8161 ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
     8221 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
     8281 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
     8341 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
     8401 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
     8461 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga atgggtggaa
     8521 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga
     8581 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
     8641 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
     8701 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct
     8761 cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat
     8821 ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
     8881 tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta
     8941 cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc
     9001 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
     9061 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag
     9121 aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca atggaagcga
     9181 tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga
     9241 tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc
     9301 agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg catttgctcc
     9361 agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca gatttccaag
     9421 cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc
     9481 cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact tcgtcacagc
     9541 ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca gagtaattga
     9601 ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgctgccag
     9661 cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat
     9721 gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc
     9781 cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg tcggaatccc
     9841 ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa ctttcaccac
     9901 gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt cagcagtggt
     9961 agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa gttgcggaag
    10021 cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg tcacacccgc
    10081 cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat ccagatacag
    10141 ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc
    10201 tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa tgtttgaacg
    10261 aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg atggccccgg
    10321 tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc
    10381 ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc tggaaggctg
    10441 cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg gtttgcttat
    10501 tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct gtccgacaca
    10561 ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga ccgttctgcg
    10621 cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg gtgaaccctt
    10681 catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag gcattcatac
    10741 tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgccctatat
    10801 cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga
    10861 cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat
    10921 cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg taaaagccat
    10981 taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac
    11041 gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct
    11101 tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag ggttaaccgc
    11161 gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt
    11221 aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac cgagcgcgcg
    11281 cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg
    11341 ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga tgagttgatc
    11401 attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg
    11461 tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc
    11521 gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca gacggtacag
    11581 gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac cggcatcatt
    11641 aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctgctg
    11701 ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat
    11761 ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga tatcatgctg
    11821 gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat ggccgcgacc
    11881 gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg cgatcttatc
    11941 gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccagttg
    12001 attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga tcagtatctc
    12061 tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga acacgccatc
    12121 gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg ctccggctcc
    12181 cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg cggacaaaat
    12241 ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat cctcgaatcc
    12301 tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt
    12361 aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat gccggcattc
    12421 aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct
    12481 tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg gggctatcgc
    12541 tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg
    12601 cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggtaac
    12661 ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta ctacacgcgt
    12721 aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt gcacgatatt
    12781 ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc
    12841 gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg caacagcctg
    12901 gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc
    12961 gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac
    13021 ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc tcgcgcttat
    13081 cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg tggtccggtt
    13141 ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt tggcgcgcaa
    13201 actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg
    13261 gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg taaactgcct
    13321 acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc aacgctggag
    13381 aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga agtgcataaa
    13441 ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat
    13501 ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt cttcaccgcg
    13561 ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatctctt
    13621 cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt
    13681 cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt
    13741 tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg acggtacttt
    13801 tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc aggaactgaa
    13861 aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt
    13921 ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga
    13981 acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg
    14041 cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt
    14101 cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa
    14161 acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc
    14221 ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg gcattatgaa
    14281 acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa
    14341 cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg
    14401 cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa
    14461 tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg
    14521 cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca gctgacctct
    14581 ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat cacatcaaat
    14641 cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt agcatagttt
    14701 cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac acattgcctg
    14761 ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag cttggcgatg
    14821 tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct gaaatcaacg
    14881 cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat atcaaagcag
    14941 gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag
    15001 gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac tatctgaacc
    15061 gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg acgctcagcg
    15121 cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag gcccgccaga
    15181 gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg
    15241 gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc cgccgccttc
    15301 cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg ctggaacgct
    15361 ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat ctggcgaaat
    15421 acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg gacaacacca
    15481 ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat atgctgcctg
    15541 gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc
    15601 cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc
    15661 cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg
    15721 aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa
    15781 atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc
    15841 ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc
    15901 cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac ctcaacattc
    15961 acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat tgatgtggga
    16021 ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct taatgcctga
    16081 tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata ttacaggtac
    16141 tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc agccgtcgtc
    16201 accccgacag tatcggggtg acggataatc acttactcga tccctttact acgcaggtaa
    16261 tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag aatgcgggtc
    16321 gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt
    16381 tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt cggttcgtcc
    16441 atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg ccctttttcc
    16501 ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag caaacgaccg
    16561 agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact catccattcg
    16621 aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta accaatgcgc
    16681 gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac cagcgttttc
    16741 agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc accgacctcc
    16801 agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt cagaccttcc
    16861 acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa cgggttctga
    16921 cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt
    16981 gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg caactcagca
    17041 atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg ggtcgccgcc
    17101 gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc cagatccgcc
    17161 atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat catggtgctg
    17221 tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag gttgttggtc
    17281 ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag aagcacacgc
    17341 agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac tggaattccc
    17401 acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat
    17461 ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg caaagcatag
    17521 atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat caccgtatcc
    17581 agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat gcgctcgttg
    17641 ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat cttcataaag
    17701 gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg
    17761 acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt actggaaact
    17821 aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat aagtctgaaa
    17881 gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg
    17941 tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca atactctaat
    18001 attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata tgaaattgaa
    18061 aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac
    18121 ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga tcaccattcc
    18181 tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg ggctttccaa
    18241 tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta ctgtactgaa
    18301 cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc
    18361 agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg
    18421 cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg agcataagaa
    18481 agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga
    18541 accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt atgcactcta
    18601 catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg gcctgcgtgt
    18661 aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc
    18721 ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg agccagacgg
    18781 cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt ttgaaggtca
    18841 ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc agccagatgt
    18901 tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg ttcgcctgaa
    18961 ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta tttgccagac
    19021 cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc cacaacagca
    19081 tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc atacgtagcg
    19141 caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt accgcccatg
    19201 ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc acgttactga
    19261 tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa tgactcacgt
    19321 tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg ataaacatcg
    19381 ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc gccagcagca
    19441 caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc agcgccgtca
    19501 gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac tccggtgttt
    19561 gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac aacagcagga
    19621 gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg gcaaacgaaa
    19681 gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc agtagcgaac
    19741 cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc tcacacagtc
    19801 tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg gtcagaaagg
    19861 tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc gtagcaaagc
    19921 ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca cctttggtca
    19981 gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca ggccaggatt
    20041 tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat aactgaaaaa
    20101 aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt gtttctgggt
    20161 aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc cttccgcgtc
    20221 gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga ctatctgatg
    20281 tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca cgccacgcca
    20341 ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag ccaccgtcgg
    20401 ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca cttccctgat
    20461 caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg cctctctgac
    20521 ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt tttttgctat
    20581 tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat acatagcgtt
    20641 ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt aacgttttag
    20701 caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat ccctcaagga
    20761 tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt atgcgcgtgt
    20821 ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc gatatggtca
    20881 acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt acgccgcttc
    20941 actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact ttttgtcgga
    21001 tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg gcggctcgct
    21061 ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct ttcccctctt
    21121 cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg tttgttacga
    21181 tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg ggtatttagc
    21241 ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt aggttacccc
    21301 ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg gcgaacattg
    21361 cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg ttatttccct
    21421 tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca ctccaccaat
    21481 atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct ggtcggctta
    21541 tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga aaaagcgcgc
    21601 tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt tgttgctccc
    21661 gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta tccggtattt
    21721 cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca agaacggatg
    21781 gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc gaagtatcag
    21841 acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc ctttggcggt
    21901 cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc tgactacatt
    21961 gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt agtcgatggc
    22021 aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt ccagacctgg
    22081 tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag catcgccaaa
    22141 cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa aaacacgaaa
    22201 gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc gcaaccgcag
    22261 ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac acaaggcaaa
    22321 tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat gacgcaaacg
    22381 gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag cttctctctg
    22441 gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt gcaatacctt
    22501 gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat ttccagcggc
    22561 gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt aggctttttc
    22621 tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt tatatctgag
    22681 aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga ttacaaccat
    22741 ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc gagaggcgga
    22801 tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca ggtgcctacg
    22861 tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg
    22921 ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc
    22981 gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta gatgcttgcc
    23041 cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt tttgttgtag
    23101 tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat cacacccagc
    23161 gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt gttcatttgg
    23221 ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc tacggaagta
    23281 cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc
    23341 acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac
    23401 tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg
    23461 ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc
    23521 cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc
    23581 attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat tgggtatatc
    23641 cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat gcctggttca
    23701 ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat ggcgtctatt
    23761 cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc gggtgtcact
    23821 gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc atggcgactg
    23881 cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct gggtgggatg
    23941 aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt ggcgctggag
    24001 ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg
    24061 gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat
    24121 gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc tatttacatt
    24181 ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat tggttcgttg
    24241 attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc actctggcac
    24301 tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct gccttattcg
    24361 ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct gatgagcatg
    24421 gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata
    24481 cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc
    24541 aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt atggttatgc
    24601 ataaccatgc agaatttctc gctacttttc ctctacaccg tattta
//
LOCUS       DAZPDE010000046        24342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPDE010000046 DAZPDE010000000
VERSION     DAZPDE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.046"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..90)
                     /locus_tag="ACHU0J_001702"
     CDS             complement(<1..90)
                     /locus_tag="ACHU0J_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216433.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLG"
     gene            complement(183..755)
                     /locus_tag="ACHU0J_001703"
     CDS             complement(183..755)
                     /locus_tag="ACHU0J_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1216434.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            973..1833
                     /gene="tesB"
                     /locus_tag="ACHU0J_001704"
     CDS             973..1833
                     /gene="tesB"
                     /locus_tag="ACHU0J_001704"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1216435.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1882..3168)
                     /gene="amtB"
                     /locus_tag="ACHU0J_001705"
     CDS             complement(1882..3168)
                     /gene="amtB"
                     /locus_tag="ACHU0J_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1216436.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3198..3536)
                     /gene="glnK"
                     /locus_tag="ACHU0J_001706"
     CDS             complement(3198..3536)
                     /gene="glnK"
                     /locus_tag="ACHU0J_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1216437.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3717..5498)
                     /locus_tag="ACHU0J_001707"
     CDS             complement(3717..5498)
                     /locus_tag="ACHU0J_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1216438.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5491..7263)
                     /locus_tag="ACHU0J_001708"
     CDS             complement(5491..7263)
                     /locus_tag="ACHU0J_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1216439.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7293..7751)
                     /gene="decR"
                     /locus_tag="ACHU0J_001709"
     CDS             complement(7293..7751)
                     /gene="decR"
                     /locus_tag="ACHU0J_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR1216440.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7905..8723)
                     /gene="cof"
                     /locus_tag="ACHU0J_001710"
     CDS             complement(7905..8723)
                     /gene="cof"
                     /locus_tag="ACHU0J_001710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1216441.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8823..10515
                     /locus_tag="ACHU0J_001711"
                     /pseudo
     CDS             8823..10515
                     /locus_tag="ACHU0J_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10580..11275
                     /gene="queC"
                     /locus_tag="ACHU0J_001712"
     CDS             10580..11275
                     /gene="queC"
                     /locus_tag="ACHU0J_001712"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1216442.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11327..11725)
                     /gene="fadM"
                     /locus_tag="ACHU0J_001713"
     CDS             complement(11327..11725)
                     /gene="fadM"
                     /locus_tag="ACHU0J_001713"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1216443.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11819..12190)
                     /locus_tag="ACHU0J_001714"
     CDS             complement(11819..12190)
                     /locus_tag="ACHU0J_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1216444.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12341..14212)
                     /gene="ppiD"
                     /locus_tag="ACHU0J_001715"
     CDS             complement(12341..14212)
                     /gene="ppiD"
                     /locus_tag="ACHU0J_001715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1216445.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14404..14694)
                     /gene="hupB"
                     /locus_tag="ACHU0J_001716"
     CDS             complement(14404..14694)
                     /gene="hupB"
                     /locus_tag="ACHU0J_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1216446.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14885..17239)
                     /gene="lon"
                     /locus_tag="ACHU0J_001717"
     CDS             complement(14885..17239)
                     /gene="lon"
                     /locus_tag="ACHU0J_001717"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1216447.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17427..18701)
                     /gene="clpX"
                     /locus_tag="ACHU0J_001718"
     CDS             complement(17427..18701)
                     /gene="clpX"
                     /locus_tag="ACHU0J_001718"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1216448.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18827..19450)
                     /gene="clpP"
                     /locus_tag="ACHU0J_001719"
     CDS             complement(18827..19450)
                     /gene="clpP"
                     /locus_tag="ACHU0J_001719"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1216449.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19697..20995)
                     /gene="tig"
                     /locus_tag="ACHU0J_001720"
     CDS             complement(19697..20995)
                     /gene="tig"
                     /locus_tag="ACHU0J_001720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1216450.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21339..21656)
                     /gene="bolA"
                     /locus_tag="ACHU0J_001721"
     CDS             complement(21339..21656)
                     /gene="bolA"
                     /locus_tag="ACHU0J_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1216451.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21961..22539
                     /locus_tag="ACHU0J_001722"
     CDS             21961..22539
                     /locus_tag="ACHU0J_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1216452.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22583..24058
                     /gene="ampG"
                     /locus_tag="ACHU0J_001723"
     CDS             22583..24058
                     /gene="ampG"
                     /locus_tag="ACHU0J_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1216453.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5716 a         6705 c         5864 g         6057 t
ORIGIN      
        1 ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg
       61 tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac
      121 tggatcggtc aactgatcct taactgatcg gcattactcc gggcggagag ggttaacgtc
      181 gcttaatatt gcgtaggagc gggaactgcc gaagacggat tcacctgagt tggtgaagtc
      241 gaaggtacgg tagtcgttgc gccaccgctg gcctgaaccg gcacggcggt ttgctgtacc
      301 ggcaccaatg tcaggtcggc tttagttccg ccctggttga tcaccggctg aacggtatcg
      361 gtgataaata ccagtttgtc attcacggta atcgccgcac tcaacagaat acgcgcgttc
      421 ggctgaacat ctgccgggtt aaatggcaga acaaagctga atggtgactg tttaccttca
      481 gtacgcaccg ctttctgcgc cagcactttt gacggtgcat cggctaacga cgcgtcagaa
      541 agtgtcacgg tcagcacagc atcaggcggc agtgcgactt tctgacggat ccagacagta
      601 ccggagacat tcggttgctg gatagcaggt tgttgtgttg ctgaaataga cgtatttgca
      661 gccggggctg gcgtctgaat atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca
      721 accgctaaac cactggccat gtgcacgagt ttcattcatt tctccttatt atcaatgcac
      781 cagcgggcta actttcctcg ccggaagagt ggttaacaaa atagtaacgt caacaagtgt
      841 ggcacacatc acgcatttct gcctgtaatt agcccgtaat tcagactatt tcacccatcg
      901 gaccagtggc aaatttgcgt tatactcaac tcactttggc ttgctgcggc agctttgtta
      961 ctggagagtt atatgagtca ggcgctaaaa aatttactga cattgttaaa tctggaaaaa
     1021 attgaggaag gactctttcg cggccagagt gaagatttag gtttacgcca ggtgtttggc
     1081 ggccaggtcg tgggtcaggc cttgtatgct gcaaaagaga ccgtcccgga agagcggctg
     1141 gtacattcgt ttcacagcta ctttcttcgc ccaggcgata gtaagaaacc gattatttat
     1201 gatgtcgaaa cgctgcgtga cggtaacagc ttcagcgccc gccgggttgc tgctattcaa
     1261 aacggcaaac cgatttttta tatgacggcc tctttccagg caccagaagc gggtttcgaa
     1321 catcaaaaaa caatgccgtc cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc
     1381 gcccaatcgc tggcgcacct gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt
     1441 ccgctggaag tccgtccggt ggagtttcat aacccactga aaggtcacgt cgcagaacca
     1501 catcgtcagg tgtggatccg cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag
     1561 tatctgctcg gttacgcttc tgatcttaac ttcctgccgg tagctctaca gccgcactgc
     1621 atcggttttc tcgaaccggg gattcagatt gccaccattg accattccat gtggttccat
     1681 cgcccgttta atttgaatga atggctgctg tatagcgtgg agagcacctc ggcgtccagc
     1741 gcacgcggct ttgtgcgcgg tgagttttat acccaagacg gcgtgctggt tgcctcgacc
     1801 gttcaggaag gggtaatgcg taatcacaat taaaaaaata gccggaggtg aaaaccgtcc
     1861 ggctattttt tgcagtgctt gttacgcgtt ataggcattc tcgccgtggc tgttgacgtc
     1921 cagcccttct cgctcttgtt cttccggcac acgtaggcca accgtcagat ccgccagttt
     1981 gtagccaata aatgccacca caccggacca gacgatcgta atggcgatgc tttccaactg
     2041 taccagcaac tgatggccca tcgtcacacc ttcagcgaag cccacgccgc ccagagaact
     2101 ggcggcaaaa atcccggtca tgatacagcc gacaatgcca caaacgccgt gcacaccgaa
     2161 gacatcgcag ggatcatcca cccgcagcaa gcgtttgagc atggtaacgc cccacaaacc
     2221 cgccagacca gctaccacgc cgataatcaa cgcgccgcca accccaatgt agccgcacgc
     2281 tggcgtcacg ccgaccagac cggcaatcgc gccagaacac gctcccagca gagaaggctt
     2341 accacgcagc gcccattcac cgaagatcca gccaagaatt gccgccgccg ttgcgaccac
     2401 agtattcaca aatgccagtg ccgcgatttc attcgccgtg cccactgacc cggcgttaaa
     2461 gccaaaccaa ccgatataga gaatggcagt cccggtgaag accatcggca ggttgtgcgg
     2521 tttaaacgcc tctttaccga agcctacgcg tttgcctatc agatacgcgc ccaccagacc
     2581 ggcgattgct gcgttaatgt gcaccaccgt gccacccgcg aaatccagcg caccgtgaga
     2641 agccagcaaa ccaccgcccc acaccatatg cgcaatcgga atgtaagaga gcgtcagcca
     2701 taccaccacg aaaatcaaca cagctgagaa gcggattcgt tccgccagcg ccccaactat
     2761 caagcctacg gtaatgcagg caaacgatcc ctgaaacgct acgtggatat actgataaat
     2821 gctgcccatc accgccgtca gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa
     2881 gaagttgttg ccctcaccaa acgccagcga ataaccgtaa accacccaga gaatacagac
     2941 cagtgcaaac gtcaccgtca cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa
     3001 cccaccgtaa aacagggcaa tccccggaat agtcataaac agcaccagcg cagtacaaat
     3061 catcataaac gcattgtcgg ctttatcggc caccgcaggt gcagccatta ccagtcccgg
     3121 cagcatcgcc agtgaagcaa gcccagtttt tatcgtcgct atcttcattt ttcgttcctg
     3181 ttgctgtgtg ccagagatta cagcgccgct tcgtcggctt cgccggtacg aatacgaatg
     3241 acgcgttgca attcagcgac gaagattttg ccgtcgccaa tttttccggt gtaagccgcc
     3301 ttactgacga tatcgatcac ttcatcgagt tggtcatcag caatcgccac atcaattttt
     3361 acttttggca ggaagttgac gctgtattcc gccccccggt acagctcggc atgccctttc
     3421 tgacgcccga aacctttcac ttcggtgacg gtcaggccct gaataccaat ggaagataac
     3481 gcttcacgaa cgtcttccag cttgaatggt ttgattatca cggtcaccag cttcatagat
     3541 cccctccggt cagaattcgg taatggttct gctacacgaa gaaggtattg caagcggtgt
     3601 gccagaaatg aaaaacgaga aagagagcag gaagttaaga agattgaaaa tgaaaacgca
     3661 ctatgacagt gcacagtgcg ttacattttt gcaccaacga tgaaatattg cgctattcag
     3721 gcgctcaatg attcctcttc acgcacgctg gctgccagct cttcgcccgc aagttgcagt
     3781 tgatacatct gccagtagcg tccctgggcc gccagcagtt gctggtgagt gccctgctcc
     3841 acggcttgcc cacgatgaag caccagaatg gtgtcggcat caataatagt tgataagcgg
     3901 tgagcaatca ccaccagcgt ggtatgttca cgcaccgccg ccagagcatg ttgaatcgcc
     3961 tgttcagtac cggagtcaat gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc
     4021 tccaccagca cgcgcgccag tgccagcagt tgcttttgcc cgactgagag attatttccc
     4081 tgctcgccca gcggcgtata aatacaatca ctcatgctgc gcgccagctc cgccagttgc
     4141 acggtttcca gcgcctgcca gacgcgttct tcggagatat cccgccccag cgtcacatta
     4201 gcgaggaagg tatccgccag caccaccgga tcttgctgca ccattgccac gccctggcgc
     4261 agcgcgctat gacttagcga acttaatgga cgaccatcaa ggtgaatctc accttccgtt
     4321 agcgggtaat agcccatcaa taaactggcg agggtacttt taccactgcc ggtatgcccg
     4381 accagcgcca caaagttacg cgaaggcaca gagagattaa tgttctttag caccagattg
     4441 tcatcgcgat aagcaaatga cacgttatcg acttcgatgg tgccactctg taacgggtaa
     4501 tcatcattgc catattgctg gcgcggtccg tccatcagtt caaacacgcg ctcaccagca
     4561 acaacagcct gttgcagcat cgcctgttgc gtggtcagtt cgattaaggg ttcgttaagt
     4621 cgcccaagat agctgataaa cgcatacaac acgcccactt caatggtgcc gctggcggag
     4681 aagccaaaca gcatcaataa gccacaaaga atgagcgatg aaaacagact cagcagcgga
     4741 cgcagcagaa aaccgtcgag gcgcagggtt tgcatcctag ccatatagtg tgaacgactg
     4801 gcctccccca tacgttcgcc aaatcgcgcc tgctgacgaa actgctggat aacgctcatg
     4861 ccattgatga tttcgttaaa gccgtcgttg atatccgcca gataggcgcg cacacggcgg
     4921 acaatcggcg tgctgtaacg ctggtatatc accattacca ccagcaccac cgggaaaatc
     4981 attatcgcca ccagcgccat tcgccagtcg aggctgaaca tcgccaccag catcgcgccc
     5041 accagtgcgg cactgcgcag gacagttgcc actacggtaa cgtagagatc gcggatcact
     5101 tcagtgtcat tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt
     5161 aatggctggc gtaacgcagc atccatcacg tcggtacgca actgttgcac tgcgcctact
     5221 gccgcccgat taaacagcag cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc
     5281 ccaacatacg ccgcagccag ccctgcaacc actttcaacg gcaggttatt tttcgctacc
     5341 atattgtcga taaaatagct gataagcagc ggcccactga cttctgccgc cgccgcaacc
     5401 cacatcatca ggaccgcaat ccccagcggt ttacgccacg gcgaaccata cgctaacagg
     5461 cgcttgagag tcggccacag ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc
     5521 cggaacgtcg tcgagcgccg cctccagttg ttgatagcga tacatatcgc gataccaacc
     5581 gctttgttgc gccagctcat catgattgcc acgctgggcg atatgtccgt gctgcatcac
     5641 aataatttca ctggcttccg tcagtgccga aaggcgatgg gcactgatga ttaccgttct
     5701 tccctgtccc cactgacgca ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc
     5761 cgaaagcgca tcatcaagga tgaggatttc cgcgttgact aataacgctc gagcaatgga
     5821 gatacgctgt ttttgcccac cggaaagcat cacgccgcgc tcgcccactt ctgtatcgta
     5881 accttgcggt agacgcaaaa tatcgtcatg tacgctggct aaccgcgcga catgctcaat
     5941 ctcttgctgg gtggcattcg ggcaacccag cgcgatgtta ttcgccacgg tgtcagaaaa
     6001 aaggaatggc gtctggctga ccaccgccag gcggctacgc cagctatcga gttgtaactt
     6061 cgtcagagga atatcatgaa agcgaatatc cccctcgctg acgtcgaaat gacgctgaat
     6121 gagcgacaac agggtacttt tgccggaacc agtcggcccg caaataccca gcatctgacc
     6181 gggtttcagg gcgaaattga cgttttccag cgcaggatgg tcagtctgcg gatacgtgaa
     6241 ctggtgaata tttacatcca gttcgccacg cccttccggc acgggttcgc taccatcgtt
     6301 caccaccggc gcttccgcca gcatggcgcg aatacggctg tatgcagcac taccacgttc
     6361 cacaatgtta aacatccatg ccagcgccag cattggccaa atcatcagac ctaaatacat
     6421 cataaaactg gtgagctggc ccagcgttaa actgccctgc accaccatcc aactaccgcc
     6481 gccaatcgcc agcaagttcg ccataccaat cgcgatatag atggtcgggt cgaagcgagc
     6541 atcaatacgc gccacccgca tgtttttttt gccggtatct tcggcatccg cggcaaataa
     6601 cgccgactgg cgatcttcca gaccaaaggc tttgatcatg cggatactgg tgaggctttc
     6661 ctgggtgcgg tcattaagac tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa
     6721 ggcatcgccg ttgcgcttaa tcatgatcgc catcactggc atcggtaaca gggcaaataa
     6781 ggtcaactgc cagctaattt gcgtagacat cataatcaac acagcgcagc ccattaccag
     6841 tgaatccacc agcgtcagca ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt
     6901 ggtcgcacga gccatgagat cacccgtgcg atgacgcagg taaaactccg ggtgctgccg
     6961 gcttagctga cggtaataat cttcacgcag ttcaacagcc agttggtaag acgcgccaaa
     7021 cagcaatacg cgccagacat aacgcaggag ataaaccaca acggcaatca gcaccatggt
     7081 ggcgatccac atcaggctca gcccggtagt aaagtgttgt tctgtcacgc catcgacaac
     7141 aataccaacc acttttggcg gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac
     7201 agccccaaga tagcgacgcc attcccgacg gaaataccag cttaattgag caaataatcg
     7261 cacgcgttgt gtcctgacct gattctggat atttattcga tgggtaaaga agtggtgtat
     7321 ttaatctgtt ccatcgcgaa gctggaagtg acgtccgaca gccccggcac gctgtttacc
     7381 agacgcttat aaaactcgtc gtagcgtttc atgtcggcaa cctggacgcg catcagataa
     7441 tcgtattcac cagccatgcg ccagaccccc agcacttctg gcatttcggt aaccaccgtg
     7501 acaaagcggc aataccattc gctgctgtga tgttgcgttt ttatcagcac aaaggcggtc
     7561 aggccgaggc ctattttttc cggatccagc agggcgactt tgccgataag gataccgtcg
     7621 tcctccagcc gtttcaggcg cttccagcag ggggtggttg tcagattaac ggcttcagcc
     7681 agtgcctgca aagagagggt gcaatcctgc tgcagtaagg ccagcagctt acggtcaatt
     7741 ttatctaaca tagcccttcc acggagaatt tttttctctt ttaattctat tttaaaggtg
     7801 aaatcgcgac aatttattct gtgcggttta aacaccacgg cacaaaatga caaattcaac
     7861 gcaaaaaaaa accgggcaat tgcccggtgc tggaagggat ctcgttattc gggggaataa
     7921 ggtagatgtg gatagtccag ccagtgcgtc aaatagtgag agacagcctg atttcggcaa
     7981 tgtccaatca ccggtaaatg cgggagctcc gcgcgcagtt gcggcatcgc attgcccata
     8041 ataaacccgc taccgacgct gcctaacatt tcgcgatcgt tcatcgcatc accaaaggcc
     8101 atgcaatcgc gcaacgataa acctaaatgt tgggtccgca ccgtcaatgc agcgccttta
     8161 ttgcagccca ccggcagcac ttcgaggcaa tccgtggcgg aaaaacacaa atgtgcacgc
     8221 tcgcctaatg cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg atcgccacag
     8281 aagcagatct tggtgacgct gccgagtggc atttttttga catcgattat ctgataacga
     8341 aaaccgctat agacaaatac ctgcaacaac gcagggctct ctttcccggt aaaccaaccg
     8401 tcgtcattga agatatgcat gctggctcgg gtatcccatt gctgatacag caccagctcc
     8461 gcgacatccg caggtaaatc atcacgatgt aaaagttcac cttccagaga atgcacgcgc
     8521 gttccgttgc cggtaatcaa atatgcatcc agcgatagcg ccccgagaat atgctgcatc
     8581 tccagcgcat gacgccccgt ggcaaaagtg agggtaatgt cgcgttcacg cagtcgcgcc
     8641 aaagtagaga gggttttctc acctaaatga tggtcaggca tcaataaagt gccatccata
     8701 tcaaatgcta ccagacgagc catttctttc tccactctgt gacacggtta atggtgattg
     8761 agtatcacct gatatatacg gaagtaatag tgaatagtta aataatattg ttccgggttt
     8821 atatgcgatt gctcaaccgt cttaaccagt atcaacgtct gtggcaacct tcagccgcaa
     8881 accgtcaccg tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg
     8941 ctgttgcgtc aggcacagga ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga
     9001 aagcgcggac aattacgctt tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa
     9061 caggcactgg aaaccggaaa gcagcaagat gtgctggagc tggcgcaact ggccccaggt
     9121 gagctgcgca ctctgttaca gccatttatg ggcggacaat ggcaaaacga tacacccacg
     9181 ttgcgtattc cctactatcg cccgctcgaa ccgctacaac caggcttttt gcccggccgt
     9241 gccgagcagc atctcgccgg gcagatattt tccggcctga cccgcttcga taataatact
     9301 cagcgcccga ttggcgattt agcgcatcac tgggaaacct ctactgacag gttacgctgg
     9361 gacttttatc ttcgttcaac cctacactgg cataacggcg atgcagtaaa agcctcacac
     9421 ttacaccagc gattattgat gctgttacaa ctgccagcac tggatcaatt atttattagc
     9481 gtgaagcgta ttgaagtcac ccatccgcag tgtctgacct tctttttaca tcgccccgat
     9541 tactggcttg cgcaccggct ggcgagctat tgcagccatc tggcgcatcc gcaattccca
     9601 ctgatcggca ctggtccttt tcgcttaaca caattcacag cggagctggt gcgcctggag
     9661 agccatgatt attaccattt gcgtcatccg ctgcttaaag cggttgagta ctggataact
     9721 ccgccgcttt tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca aatcaccatc
     9781 ggcaaaccgg aggagctgca acgggtcagc caagtcagta gtggcatcag tttaggtttt
     9841 tgctatttaa cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg aaaagtgatc
     9901 tccattattc atcaatccgg tttattacaa acgttagaag tcggagaaaa cctgatcacc
     9961 gccagtcatg cattactgcc aggctggact attccgcact ggcaagtacc ggatgaagtc
    10021 aaactaccga aaaccttgac gctggtttat cacctaccga tagaacttca taccatggca
    10081 gaacgcctac aggcgacact ggcagcggaa ggctgtgaac tcacaattat ttttcataac
    10141 gcaaaaaact gggacgacac gaccctactg gcacacgcag acctcatgat gggcgacaga
    10201 ttaattggcg aagcaccgga atatactctg gagcaatggc tacgttgcga tccactatgg
    10261 ccacatgttt tcgacgctcc agcatatgca catctgcaat cgacgctgga cgcggtgcaa
    10321 gtaatgcctg atgaggaaaa ccgatttaat gccctgaaag cggtttttag ccagttaatg
    10381 gcagacgcga cgctgacgcc gctgttcaac tatcactatc gcattagtgc ccctcccggc
    10441 gtgaacggtg tgcgactgac accgcgcggc tggtttgaat ttaccgaagc ctggcttccc
    10501 gcgccgtcgc aatgaatagc tggtccgggt taatcgcagg cgttaccata acgcttttat
    10561 tgttcaagca ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag gccaggattc
    10621 caccacctgt ctggtgcagg cattacaaca atatgatgaa gtccattgcg tgacgttcga
    10681 ttacggtcag cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc tgaaactggg
    10741 agcacgcgcg cataaggttc ttgatgtaac gctgctcaac gagctggcgg tcagcagcct
    10801 gacacgtgac agcattccgg tgcctgatta tgaacctgaa gccgatggca tcccgaatac
    10861 gtttgtccca gggcgtaata ttttgttcct gacgctggcg gcaatatatg cgtatcaggt
    10921 aaaagcagaa gccgtaatta ccggcgtctg tgaaacggat ttctccggct acccggattg
    10981 ccgcgatgag tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg cgaaagatat
    11041 tcgttttgaa acgccgctga tgtggattga taaagcggaa acctgggcgc tggcagatta
    11101 ttacggcaaa ctggatttag tccgtaacga aacgttgacc tgctataacg gcattaaagg
    11161 cgacggttgc ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga atcattatct
    11221 ggccgataaa ccgacggtga tggcagcgat gaagcagaaa accgggttga ggtaattatt
    11281 ccgggtgtcg ccggatgcgg cttgagcatc cggcaccaca aaacgtttac ttaaccatct
    11341 gctccagctt ctcgcgcaat tccccttcca gagctaatgc tttctgcgtt ttaagatcaa
    11401 tacaaacaaa cgtaataagc gcatccgcta ccacctgccc ttccggctcc agtgtaatga
    11461 cctggtttaa gatgccgctt ttaccgttta attgctgcaa ctgactggta atagttaaca
    11521 ggtcgcttaa taccgctgga cgacgatagt taatattgat attgaccacg acgaaggcga
    11581 tgttatgggc cgtcatccac tgaaaactgt cgctattttc caccccatcc cagcgggctt
    11641 cttcgagaaa ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg agatgatatc
    11701 cacgaacttt gatttgtgtt tgcatagcgc aataacctta cgttgttgtt ataagtacag
    11761 aggtcataac tggtatgacc tcttcagtct ggcaaaaaat tgccactatg caaattaatt
    11821 acagggttaa taccgccaga ttacgttcca ccagcgaatt gcccatcccc ggcacctgct
    11881 ttagatcctc caccgtttta aacggaccgt actcttcgcg ataactgaca atcgcctgcg
    11941 ctttcttcag gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg gcattattaa
    12001 tgctgacccg ggtgccttct tcgtcactgg ctttcgccgg tactgctgct ttactttgtg
    12061 ctgcaggagc ttccgctttg gtttctaccg ccgtcggttt cgccacagaa gctgccgcca
    12121 gcgcgctatg agacattccg gcacaggcca gggacagtgt aatgagcagt gctttaattc
    12181 cgtgtttcat actgttttct ccttgtttgt taacagtgga atcaccttaa cgggagtgaa
    12241 aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga ccttttcttg
    12301 ttacagctgc aacgcatttt tttgcgaggc ggcttcagga ttattgctgt tccagcgcat
    12361 cgccaatttt gattttcgcc tctttacgca ggttactcat cagagcttca aagacgattt
    12421 gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt ctgatcttcc ggcattgaac
    12481 cttgtttcac ttcatccagc gccagcagaa ccacattacc ttgcatatcg gtcgccatac
    12541 cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg ctaatcgggt
    12601 cacgaccgga acggcttaag gttttcggct cgccaaattt cagaccggca gcctgcatag
    12661 cttccgcacc tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc actttcgcct
    12721 gttgttcagc tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca tctgccaacg
    12781 gtttcaccgc ttccggtttg tgctcgctga cgcgcagcac gaatgcgcgg tcgccgtcta
    12841 cggtgatgat gtcagagttg atgcccgacg cgccgttttc acctaccaga ccgccgttaa
    12901 agatagcgtc ggcaaccggc ttgaagttca actcttccgg caggttatct ttgctgaacc
    12961 aacccgtctg agtggcttta acgccggcag cttgctctgc accggccaga gactcggtgt
    13021 cgttgcttgc cacatcgctc actttctgct gcagcgcata gtacgcatcg agggcttttt
    13081 cgtgtttcac tttcgccgca atgtcgtcac gtacttcgtc taacgatttc actttcgctg
    13141 gctgaatgtc gtccagacgt acaatcagga aaccgaccga agatttgatg acaccagaca
    13201 gttggccttt ttctttcaga ccagcatttt tcagttcgtc ggggatagtg gcatcttcta
    13261 accaacccat atcgccgccg ttacgggcag agataatatc ggcagatttt tctttggcta
    13321 atgcagcaaa atcaccgcct ttattcagct catcaagtac cgctttcgct tcatcttcag
    13381 ttttggtctg gatgatgctg tagcgggtac gctgcggctg ggtgaattga tcctgatgct
    13441 gatcgtagta gctctggata tccgcatcgc taaccggttg ctgcatcgtt gcggcaacca
    13501 gcttgatgta actcacgcgg aattgttccg gcgtcatgaa attgtttttg ttttgttcgt
    13561 agtagctggc aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg ttaacatcga
    13621 tagtcgcctc acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg tcggtttcac
    13681 ctttcagcat aaaatcggta ccggcaacgc cgttaatcag ctgttgggta gtgagctggt
    13741 tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat ctggttgagg ataccgttat
    13801 agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa atcgcctgtt
    13861 taacctgctc atcactgata cccagtttca gctcacgagc gtactgatcc aacagcgcct
    13921 cgtcgatcag acgattcagc acctgttgac gcagggtttt catatagcct tcgttcgctg
    13981 ccagctcgga gtattgatcg cccagctgtt gctgcatacg attacgctcg ctgttaaagg
    14041 cgttctcgaa ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg tagttattgc
    14101 ctccgccaat caggtaacca ctcacgccgg tcaatatgaa cgacacgata atgataccga
    14161 aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc atggtgtaac
    14221 aacactccgc tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg caaaggccta
    14281 ttgtgacaag aaacgggggc aattgtcagc ccacaagaca cagaaagtca ccgaatacaa
    14341 ataaaaaagg cacatcagta gatgcgccct tgaacttcgt cacatcccca ctggggacaa
    14401 cgcttagttt accgcgtctt tcagtgcttt acctgcacgg aagctcggta ctttagcggc
    14461 agcgatggtg atctctttac cggtctgcgg gttgcggcca gtacgggcag cacgctcttt
    14521 aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc ccttctttca gagattcagt
    14581 tacggaagca ataatagcat ctaacgcacg gccagccgca gctttagaga tatcagcccc
    14641 tgcagcaatc ttgtcgatca attgagattt attcactctt ctcttcctct ttataattta
    14701 tatcgcactt gaatccttca aggtacgaac gcgcagcagt tatatcaggc cagccatccc
    14761 cttacaacac cccttaataa gggcaagccc gatccgctat ctaacttagc gagacaaaaa
    14821 aaggctggca agcccgaatt agcctgccag ccctgttttt attagtgcat tttgcgcgag
    14881 gtcactattt tgcagtcaca acctgcatgc cagacggttc attttgcagc gccagagtca
    14941 gaacttcctc aatgcgcttc acaggatgaa tgtccagatc ggcaattacg ttgtcaggaa
    15001 tctcttccag atcgcgttta ttttcgaacg gaattagcac tgttttaatc ccgccgcgat
    15061 gcgctgccag gagtttttct ttcaaaccac cgatcggcag tacctgacca cgcagagtga
    15121 tctcaccggt cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa gaaaccagcg
    15181 cggtgcacat agcaataccg gcactcggac catctttcgg cgtcgcacct tccggtacgt
    15241 ggacgtggat gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt tccgcacgcg
    15301 cacgaaccac cgttaacgcc gcctgaatag actcctgcat cacttcgccg agcgaaccgg
    15361 tataggtcag tttgccttta cccggaacac atgcggtttc aatggtcagc aagtcaccgc
    15421 ccacttccgt ccacgccaga ccgattacct gaccgacacg gttttcgtta tcagcgcgac
    15481 catagtcgaa acgctgaaca ccgaggtagt catgcaggtt atcgccgtta atttcgatat
    15541 gttttaatga cttatcgagc agtaactgct taaccgcttt gcggcacagt ttggagattt
    15601 cacgctccag accacgcacg cccgcctcac gggtgtagta acgaataatg ccgataatgg
    15661 cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt acgttcaatc tgcttcggca
    15721 gcaggtgacg tttggcgatg ttcagttttt catcttcggt ataaccggag aggcgaatca
    15781 cttccatacg atccagcagc ggtgccggaa tgttcatgga gttcgacgtc gcgacaaaca
    15841 tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc gctgaacgct acgttctgct
    15901 ctggatccag cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca gaagacattt
    15961 tgtcgatctc atcgagcagg aacagcggat ttttcacgcc cactttcgcc attttctgga
    16021 tcagtttacc cggcatagaa ccgatgtaag tacggcggtg accacggatt tccgcttcat
    16081 cacgcacgcc gcccagcgcc atacggacat atttacgccc ggtggctttg gcaatggact
    16141 gaccaagaga ggttttacct acccccggcg gccctaccag gcagaggatc ggtcccttga
    16201 ttttgttgac acggctttga accgcaaggt actcaaggat gcgatctttc acgcgctcca
    16261 gaccataatg gtcggtatca aggatttcct gcgcctgacg caggtctttt ttgaccttgc
    16321 tgcgcgcatt ccacggtacc tgtaccatcc agtcgatata accacgcact acggtcgctt
    16381 ctgccgacat cggagacatc attttcagct tctgcaactc tgcttccgct ttctcttttg
    16441 cctctttcgg catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt tcgtccggca
    16501 cgtcgtccat ttcaccgagt tctttctgaa tagctttcat ttgctcgttc agatagtact
    16561 cacgctggga tttctccatc tgctttttaa cgcggttgcg aatgcgtttc tcaacctgca
    16621 gcagatcgat ttccgattcc atcattgcca tcagatattc cagacgttcg ttaacgtcgg
    16681 acatctccag aacggactgt ttgtcagcca gtttcagcgg catatgtgca gcaatggtat
    16741 ccgccagacg cgccggatcg tcgatgctat tcagcgacgt cagcacttct ggtgggattt
    16801 ttttgttcag cttgatgtag ccttcgaact ggctgattgc agtacgcacc agcacttcct
    16861 gttcccgctc atcaatggtc ggcgactcca gatactccgc cttcgcagaa aagtgttcgc
    16921 cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc actttgacgg
    16981 tgccgtcagg cagtttcagc atctgcaata tagaggccac ggtcccgacg gtgaaaagat
    17041 cgtttacacc cggctcatcc gttgaagctt ctttctgcgc gaccagcata atttttttat
    17101 catggtccat cgccgcttcc agacaacgga tagatttttc ccgcccgaca aataagggga
    17161 tgaccatgtg cggataaacc accacatcgc gcagcggcaa tacggggatt tcaatgcgtt
    17221 cagaacgctc aggattcata gagctctctc ttagtttaat ttccgccagg taatcagatg
    17281 acacgactgt gcttcacgcc atctattaac atgtacgtca gtatatgggg atgtttcccc
    17341 cacattcaac gccgagaata gaggaaaaat taaaggggag ataaaatccc ccctttttgg
    17401 ttaactaatt gtatgggaat ggttaattat tcaccagatg cctgttgcgc ttccggctta
    17461 ccataaatca gcaacggttt gctttgacca tcaattaccg actcgtcgat aaccactttt
    17521 tcgacgtctt ccatggacgg cagatcgtac atggtatcga gcagtgcggc ttctacgatg
    17581 gaacgcaggc cacgggcacc ggttttacgc gccatcgctt tcttagcgat agcatccagc
    17641 gcctcgtcac ggaattccag atccacgcct tccagattaa acagcgcctg atactgcttg
    17701 gtcagggcgt ttttcggctc tttgaggatc tgaatcagag cttcttcgct cagttcattc
    17761 aacgttgcta caaccggcag acgaccaatg aactcaggga taagaccaaa cttgatcaga
    17821 tcttccggtt caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga cttcgctttt
    17881 accgtcgcgc caaaaccaat gccggagccg gtttctacac ggtgggaaat cactttatcc
    17941 agaccggcaa acgcaccgcc acaaataaac aggatcttag aggtatcaac ctgcaagaat
    18001 tcctgctgcg gatgtttacg cccaccttgc ggtggaacag cagctaccgt accttcgatc
    18061 agtttcaaca gtgcctgctg tactccttca ccggaaacgt ctcgggtaat ggacgggttg
    18121 tctgacttac gagaaatctt gtcgatttca tcgatgtaga caatcccgcg ctgcgctttc
    18181 tgtacgtcgt aatcgcactt ttgcaacagc ttctgaatga tgttttcaac gtcttcgccc
    18241 acataaccgg cttcggtcag cgtggttgcg tcggccatgg tgaacgggac gtccagcagg
    18301 cgcgccagcg tttcagccag cagcgtttta ccggaaccgg tcggaccgat cagcagaatg
    18361 ttacttttgc ccaactcgac accattgctg gtatcgccgt tgcgcagacg tttgtaatgg
    18421 ttgtataccg cgaccgccag cacttttttc gcctgttcct ggccgataac gtaatcgtcc
    18481 aggtggttgc gaatttcatg cggcgtcggt agcgcactgc gttcacgatg cggtgcaact
    18541 tctttaatct cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc gcagatatac
    18601 acggatggac cggcaatcag cttgcgcact tcatgctggc ttttgccgca aaaagagcaa
    18661 tacagcaatt tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa cctcttcttt
    18721 gttctttgtg ccgcacacga cgacgcaaat gccattctca ggcgcaagcc gcttacagcg
    18781 ttgtgccgcc ctagataagt atagcggcac agttgcgcct ctggcatcaa ttacgatggg
    18841 tcagaatcga atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg aagcgatcgc
    18901 gctcggtatc acgttcaatc tgttctaatg attgacccgt atgaagcgcc ataagttcat
    18961 tcatgcgccc tttaactttc agaatttcac gggcatgaat ttcgatatcg gtcgcctggc
    19021 cctggtagcc gcccaacggt tggtgaatca tcacgcgcga attcggcagg caaaaacgtt
    19081 taccttttgc ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg cccatacaga
    19141 tggtgctgac atcaggcttg ataaactgca tggtgtcata gatagacatc ccggcagtga
    19201 tcaccccgcc tggggagtta atgtacagat agatatcttt ttctgggttt tccgcttcca
    19261 ggaacagcat ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg ccagtcagaa
    19321 aaatgacgcg ttccttaagt agacgagaat agatatcaaa agagcgctca ccgcgtgagg
    19381 tctgttcaat gaccatcggc accagcgcca tatggggtgc aaagttatct cgttcgccgc
    19441 tgtatgacat ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc gattgtaacc
    19501 ttatggacgg attatcagct agtccctgtg ttatgggtac ggcatcacca tatttcaagc
    19561 ataacaatct tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ctccatgcaa
    19621 aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc gaatttagcg
    19681 cgttatgctg cgtaaattac gcctgctggt tcatcagctc gttgaaagtg gtttcttttt
    19741 cagtcacttt cgctttcgcc agtacagctt caacagcctg ttcttccaga gcaacattgc
    19801 gcatgttgtc catcagttct ttgtttttgc tgtagaactc gataacttct ttcggatctt
    19861 cgtacgcaga agccatctct tcgatcaggc ctttcacgcg ctcttcgtca gctttcagct
    19921 cgttggtgcg gataacttcg cccagcagca ggccaacaac tacgcggcgt ttagcctgtt
    19981 cttcgaacag ttcgcgcggc agttccagag cttgtttttc gttgccaccg aaacgctgtg
    20041 cagcctggcg acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt acgtcgatgt
    20101 cgttagcttt taccagacct tcgatcgcct gagacttaac gcggttacgg atggcgctct
    20161 tcagctcgcg ctccatgttt ttacgcactt cagcgcgcag accttctacg gaaccatctt
    20221 caacgccgaa acgtttgatg aattctgcag tcagttccgg cagttcacgc tcttcaactt
    20281 tcttcaggtt gatagcgaat ttcgctgctt tacctttcag gttttctgcg tggtattctt
    20341 ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt gtggcctttg ataccgtctt
    20401 caaagcccgg gatcatacga ccctggccca tcgccagtac gaaatcagac gctttaccgc
    20461 cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg tcttctgctt
    20521 caacagcgcc gtctttttct ttccaggtcg cctgctgttt acgcagagta tccagcatgc
    20581 cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc aacttcgatc gcttccagac
    20641 cctgcagttc aacttccgga taaacttcaa actctacaga gtaagtgaag tcttcaccca
    20701 gcttgtattc gcccggaaca taagtcggtg cgccagccgg attgattttt tctttaatga
    20761 tggcgtcaat gaagttacgg ctcatcaggt cacccagaac gtcctggcgt acagacgcgc
    20821 cataacgctg agcaacgata ttcattggca ctttgccttt gcggaagccg tcaatacgta
    20881 cttttttcgc aacgttgacc agctcgcttt taacagcggt ctcgatgctg tcagcagcga
    20941 tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc aactgaaact tgcatcttgt
    21001 tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg caagcacagg ttgttggctt
    21061 cgcggaaccg ggatgttctt ataatcaatc acattccctg ttaccagaat catcccgaaa
    21121 gcattcaaat aggacgcggc attatagcgg catcactttt atgagtcgag aacggttatt
    21181 gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt tcgtctgaat gctgggcaaa
    21241 aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg tttttcataa aatctttagc
    21301 aacatactgg aaaagcgccg acagttgcaa atgcgttttt acgcgatgct tcctgctcca
    21361 cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt aatagtgtaa
    21421 gtatgcagag ccagcgcatg aacggtagta gagagttcct ccgctaaagt actgtaaatc
    21481 attcgatgac gattcagaaa acgttcaccc gtaaaacgat cgctgaccag cacaacttta
    21541 aaatggcttt cagagccggc tgggacattg tgacgatagc tttcatccac tacttcgagg
    21601 aatacgggtt ggaacgccgc ccttaatttt tcttctatcc gctcacgtat catcatgaaa
    21661 tatctcctcc gacaacgctg agatgtcatc catcccttta aatactagcc gcttttaccg
    21721 tttccattgc agcttaacaa caaatattta ggtttcgtcc tggtttttcc gtcaagcgta
    21781 tgaagtttac tgatttaaag attgaaacac aggcctcgca tcagcgtttc ttttaccagg
    21841 ggtgattatt tcgtcaacat gatgaattta catgccttac ccacttcccc tcgccgttgg
    21901 caatgttatg atggcgggaa tttttaccaa acacgcttaa gagtctctga gagcccgaac
    21961 atgttcaaaa aaatcctctt cccgttagtt gctctgttta tgcttgcagg atgcgcaaaa
    22021 ccgccaacaa ctattgaagt ttccccgacg attacactgc cacagcagga tccaagcctg
    22081 atgggcgtca ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc gctggcaaaa
    22141 gtcacccgcg ataatcaaat cgttaccctg accgcctccc gcgctctgcg tttcctgctg
    22201 caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc gaatggcccg
    22261 gttaatctgc aaatcatcgt tagccaactg tatgctgacg tgtcccaggg caacgtgcgc
    22321 tacaacatcg ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa tggcaacaaa
    22381 atgaccaaaa actatcgtgc cagctacaac gttgaaggtg cgttccaggc ctccaacaaa
    22441 aatatcgccg atgcggttaa cagtgtgctg accgatacca tcgctgatat gtctcaggac
    22501 accagcattc acgaattcat caagcagaac gcgcgttaat ttctgccctc tggcccggtg
    22561 caagccgggc ctgtagacgc ccatgtccag tcaatattta cgtatttttc aacagccgcg
    22621 ttcagccata ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc tgacatccgg
    22681 caccttacag gcctggatga cggtcgaaaa tatcgatctc aaaaccattg gtttcttctc
    22741 tctggtaggc caggcttacg tttttaaatt cctctggtca ccgctgatgg accgctacac
    22801 gcccccattt tttgggcggc ggcgcggttg gctgctcgcc acgcaaatcc tgttattagt
    22861 cgccattgcg gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga tggcggcgct
    22921 ggcggtggtg atcgcttttt gctctgcctc ccaggatatt gtcttcgatg cgtggaaaac
    22981 cgatgtgctc ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc tgggttaccg
    23041 tttagggatg ctggtttccg gcggcctagc cctgtggctg gcagataaat ggttgggctg
    23101 gcagggcatg tactggttga tggcggcgct gttgatcccc tgtattatcg cgacgttgct
    23161 tgcaccagaa ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag cggttgttgc
    23221 acctctgcga gatttctttg gtcgcaataa tgcctggctt attttgcttc ttatcgtgct
    23281 gtataagctg ggcgacgcat tcgccatgag cctgacaacc acgtttttga ttcgcggcgt
    23341 cgggtttgat gcgggtgaag taggcgtggt aaacaaaacg cttggcttac tggcgaccat
    23401 tgttggcgca ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc gggcgctgct
    23461 gattttcggc attttacaag gtgcgtctaa cgctggttac tggctgctgt cgattactga
    23521 taagcatctc tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg gcgggatggg
    23581 cacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt ccgctactca
    23641 atttgccctg ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc ccgtggcggg
    23701 ttggtttgtt gaagcacacg gctggtcgac attctatcta ttctccgtcg ccgctgccgt
    23761 accggggctt attttgctgc tggtttgccg ccagacgctt gaatatacac gagtaaatga
    23821 caactttatc tcccgtaccg aatatccggc aggttatgcc tttgccatgt ggacactggc
    23881 ggcgggcgtc agcctgttgg ccgtgtggtt acttctgttg acgatggacg cgctggattt
    23941 gacgcacttc tctttcctgc ctgctctgct ggaagtcggg gttttagtcg ccctttctgg
    24001 cgtcgtgctt ggtggtttgc tggattatct ggcgctacga aaaacgcatc tgacgtaatc
    24061 tgtaaatatt atttagctgt agtaatcact cgccgtaatt attacggcta aataaacatc
    24121 agcatcgctt attaatattt gttcttttgt gcggcttagc gtttggaaat tattgacgcc
    24181 atttataaat tctatttttc ttacacgatt cagctaatga gtctttattt tctcaccacc
    24241 cagttgtcac tttaatgata attatttgtt aaataattgt tttatttcgc attggttata
    24301 ccaattgccc accaagactc cttctgcact ccccttttgt ta
//
LOCUS       DAZPDE010000047        24089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPDE010000047 DAZPDE010000000
VERSION     DAZPDE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.047"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHU0J_001724"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHU0J_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1216454.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHU0J_001725"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHU0J_001725"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1216455.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHU0J_001726"
     CDS             1531..2478
                     /locus_tag="ACHU0J_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR1216456.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHU0J_001727"
     CDS             2548..3354
                     /locus_tag="ACHU0J_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1216457.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHU0J_001728"
     CDS             3364..3771
                     /locus_tag="ACHU0J_001728"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1216458.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACHU0J_001729"
     CDS             3771..4265
                     /locus_tag="ACHU0J_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR1216459.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACHU0J_001730"
     CDS             4331..5128
                     /locus_tag="ACHU0J_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1216460.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACHU0J_001731"
     CDS             5139..5927
                     /locus_tag="ACHU0J_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR1216461.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACHU0J_001732"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACHU0J_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR1216462.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACHU0J_001733"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACHU0J_001733"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1216463.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACHU0J_001734"
     CDS             complement(8370..10001)
                     /locus_tag="ACHU0J_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1216464.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHU0J_001735"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHU0J_001735"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1216465.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACHU0J_001736"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACHU0J_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1216466.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="ACHU0J_001737"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="ACHU0J_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="ACHU0J_001738"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="ACHU0J_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="ACHU0J_001739"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="ACHU0J_001739"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR1216467.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="ACHU0J_001740"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="ACHU0J_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="ACHU0J_001741"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="ACHU0J_001741"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1216468.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="ACHU0J_001742"
     CDS             23609..24052
                     /locus_tag="ACHU0J_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1216469.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6146 c         6108 g         5891 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAZPDE010000048        23339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPDE010000048 DAZPDE010000000
VERSION     DAZPDE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.048"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHU0J_001743"
     CDS             <1..246
                     /locus_tag="ACHU0J_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1216470.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHU0J_001744"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHU0J_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1216471.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHU0J_001745"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHU0J_001745"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1216472.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHU0J_001746"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHU0J_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR1216473.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHU0J_001747"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHU0J_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1216474.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHU0J_001748"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHU0J_001748"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1216475.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHU0J_001749"
     CDS             complement(4442..5650)
                     /locus_tag="ACHU0J_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216476.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHU0J_001750"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHU0J_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1216477.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHU0J_001751"
     CDS             complement(6445..8130)
                     /locus_tag="ACHU0J_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1216478.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHU0J_001752"
     CDS             8400..8777
                     /locus_tag="ACHU0J_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1216479.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHU0J_001753"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHU0J_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1216480.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHU0J_001754"
     CDS             9224..9511
                     /locus_tag="ACHU0J_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1216481.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHU0J_001755"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHU0J_001755"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1216482.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHU0J_001756"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHU0J_001756"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1216483.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHU0J_001757"
     CDS             11268..11744
                     /locus_tag="ACHU0J_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1216484.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHU0J_001758"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHU0J_001758"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1216485.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14469
                     /gene="potG"
                     /locus_tag="ACHU0J_001759"
     CDS             13303..14469
                     /gene="potG"
                     /locus_tag="ACHU0J_001759"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1216486.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14479..15432
                     /gene="potH"
                     /locus_tag="ACHU0J_001760"
     CDS             14479..15432
                     /gene="potH"
                     /locus_tag="ACHU0J_001760"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1216487.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15429..16274
                     /gene="potI"
                     /locus_tag="ACHU0J_001761"
     CDS             15429..16274
                     /gene="potI"
                     /locus_tag="ACHU0J_001761"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1216488.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16334..16822
                     /locus_tag="ACHU0J_001762"
     CDS             16334..16822
                     /locus_tag="ACHU0J_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1216489.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHU0J_001763"
     CDS             16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHU0J_001763"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1216490.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHU0J_001764"
     CDS             complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHU0J_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1216491.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHU0J_001765"
     CDS             complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHU0J_001765"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1216492.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHU0J_001766"
     CDS             complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHU0J_001766"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1216493.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHU0J_001767"
     CDS             complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHU0J_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1216494.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHU0J_001768"
     CDS             complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHU0J_001768"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1216495.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22274..22789)
                     /locus_tag="ACHU0J_001769"
     CDS             complement(22274..22789)
                     /locus_tag="ACHU0J_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1216496.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5735 a         5756 c         6029 g         5819 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccctagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctggg cgcatccggc
    13501 tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc tgccggacag
    13561 ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc catcaatatg
    13621 atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat cgcttttggc
    13681 ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga gatgctcggc
    13741 ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg tggtcagcga
    13801 cagcgtgtgg ccctggcccg aagccttgcc aagcgcccga aactattact gctcgacgag
    13861 ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt ggtggatatt
    13921 ctggagcgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga ggcgatgacc
    13981 atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg cgaaccggaa
    14041 gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc ggtaaacgtc
    14101 tttgaaggcg tactcaaaga gcgtcaggaa gatggcttgg tgcttgattc gccggggctg
    14161 gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc ggtacatgtg
    14221 gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg ttgtaacttc
    14281 gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta tcacgttcgt
    14341 ctgaaaagtg ggcagatgat tagtgcccag ctacaaaacg cccatcgtca tcgtaaaggg
    14401 ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg tgtggtgctg
    14461 acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa accgccgggc
    14521 ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact ggtcattgcg
    14581 ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt ctttaaaata
    14641 agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga gtgggctgac
    14701 gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga cgatccgctc
    14761 tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt ttgctgttta
    14821 ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac ccgtaatatt
    14881 ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt ttatgcttgg
    14941 atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct gggggttatc
    15001 gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat tgtttacgcc
    15061 tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga ttattcgctg
    15121 gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg
    15181 ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc ggctgtgggc
    15241 gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg gcgcgtgcta
    15301 tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc gatcatcatg
    15361 ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa aagcgtggga
    15421 gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg attttgctgc
    15481 tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg tttaacagct
    15541 cgaagctggt gacggtgtgg ggcggctggt caacgcgctg gtatggtgag ttattgcgcg
    15601 atgacgcgat gatgagtgcg gttggttcaa gcctgacaat tgctgcctgt gcggcaacgg
    15661 cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc aggtttcgcg
    15721 gatcaaatgg ctttgccttt atgatcaccg cgccgctagt aatgccagat gtcatcacgg
    15781 gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg cctgcggacc
    15841 gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat gtggcggtcg
    15901 ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg atggatctcg
    15961 gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg cccgcgatca
    16021 tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc gccagctttg
    16081 tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg cggatggggg
    16141 tgaatccgga aatcaacgcc ctggcaacat tgatcctcgg cgcggtcgga attgtcggat
    16201 ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac atccaacgtg
    16261 caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc gcgcatgtcg
    16321 cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat cttcatctca
    16381 tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta ttatgatccg
    16441 tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat
    16501 tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc tggtgctggt
    16561 tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct
    16621 atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg
    16681 ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat
    16741 gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct cgaccagtcg
    16801 gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat
    16861 gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc
    16921 cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc gccgattttc
    16981 cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt aataaagcca
    17041 aaatggtggt gagtggtagc gttgaaaaaa cactgctcgg tatgctgcat cgcgatggca
    17101 cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc gtttttgcgg
    17161 cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg cgtaagcgtg
    17221 gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg atgctgcgct
    17281 ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg tggttacacg
    17341 aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat atggcgatta
    17401 tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag cgttttaatg
    17461 acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg gtcgccagcc
    17521 agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat atgtgggatc
    17581 tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg cagttaaccg
    17641 ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct gaactgggct
    17701 taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct cagggggagg
    17761 tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg ctgtgtgatt
    17821 atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc caaaccatgg
    17881 cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt tttgatatgt
    17941 tcccgcacac cgcgcactat gaagtgttga cgctgctggt gaagcaataa aaaagccaca
    18001 ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg gcgtgaacgc
    18061 cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg tgggccggat
    18121 aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg cggaaaccac
    18181 tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc gttattcagt
    18241 ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt gccgaagtat
    18301 tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt cagccattcg
    18361 tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag atcgataaag
    18421 gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc ctgaatatat
    18481 ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc ggcaaacgtt
    18541 ttgtaggtat cttttttgga aatcacgacg gctgagtttt catagtaggg tgtggtaaac
    18601 gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc gtcatatttt
    18661 ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt acattctgcc
    18721 tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat ctcattatta
    18781 gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat tttctctgcg
    18841 gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt tttcatgatg
    18901 gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg ataacaatgc
    18961 catcaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa taaaaaaaga
    19021 cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat gtaggccgga
    19081 taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc gacgctggcg
    19141 cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta attccgccgt
    19201 tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg
    19261 accaagtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg tccgtacaac
    19321 aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga ggtacttttg
    19381 aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc atacggcagc
    19441 aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg ccactgacct
    19501 tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc actgttcagc
    19561 gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag tgccggatac
    19621 tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg caccagcagc
    19681 ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac cggcgttttc
    19741 agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg
    19801 gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc aaacattagc
    19861 tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga
    19921 gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat ggttcctggg
    19981 tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc aaactgacca
    20041 gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc gcatgacgcc
    20101 acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc agcgcctgac
    20161 cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt tgcgaggcat
    20221 aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc acgtcgaagt
    20281 tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg aagccatccg
    20341 aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc agaatttctg
    20401 gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga cgccatttcg
    20461 ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat gcacaaacgg
    20521 caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt
    20581 acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc actttttcca
    20641 gcgcagtgtt gagtttctgc tgcagttcag tgtttccctg acgtaccgcg atgccgaggc
    20701 cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt tttgggttat
    20761 ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata cgcccgtttt
    20821 gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt tccgggtgct
    20881 tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca
    20941 gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag ttgtcatagt
    21001 acggggtagt aaacagtacc tgcttttcac gctccggagt gatatccatg cccgccatca
    21061 ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga
    21121 aagtacaggt tgcatcaatc tctttacaca gtgcctgcgc caggtcgacg tcaaaaccaa
    21181 cgatctggtt gtttgcatca atcgattcaa acggaggata gggggcttcg gtagcaaaac
    21241 gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg gcaatcagaa
    21301 cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa atgcttcggt
    21361 ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat tttccatata
    21421 caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga tcacctgggt
    21481 aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa tttccgggtc
    21541 cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca acgcacgggc
    21601 aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat cgctataagg
    21661 tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat ctttactcaa
    21721 ccccagtaca cggcagggcg cttcaatcag gttttgctgc acggtcagat gcggccacag
    21781 gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga
    21841 gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat
    21901 ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa
    21961 caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc
    22021 gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac tggcagtctg
    22081 atagctattg aggtcgaaga tagtaccttt aacagaataa ttatgcaata tttatgcttt
    22141 aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg ggggcggtgg
    22201 tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg gggtttattg
    22261 gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc gtggcatgaa
    22321 cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc tgaatcatta
    22381 acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat ggaatgttac
    22441 gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct ggcaaggtgg
    22501 ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg cggctggcat
    22561 cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta atgtcattgc
    22621 tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc cctccctgga
    22681 cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg gtacaggcac
    22741 tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata atcatttcct
    22801 tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt taatacattg
    22861 tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc attgctggcg
    22921 acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata gacagtgtaa
    22981 accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt aaatatgata
    23041 gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca agaagccagt
    23101 ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta caaaattttt
    23161 cacatgcgat aagagatcct gcgattaaga tgattgccaa aattacgttg tatttttgaa
    23221 aatgagcgaa atttgatagt cccagctttg actctcgata atattattta tgctacaatt
    23281 gtgggtaaag aatttaagct aatgaatttg agtgaagaat aaattaaatt gcttcagta
//
LOCUS       DAZPDE010000049        22852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPDE010000049 DAZPDE010000000
VERSION     DAZPDE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.049"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            139..1161
                     /locus_tag="ACHU0J_001770"
     CDS             139..1161
                     /locus_tag="ACHU0J_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR1216497.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1161..2141
                     /locus_tag="ACHU0J_001771"
     CDS             1161..2141
                     /locus_tag="ACHU0J_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR1216498.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2138..2896
                     /locus_tag="ACHU0J_001772"
     CDS             2138..2896
                     /locus_tag="ACHU0J_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1216499.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            2906..3715
                     /locus_tag="ACHU0J_001773"
     CDS             2906..3715
                     /locus_tag="ACHU0J_001773"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1216500.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3715..4569
                     /gene="modD"
                     /locus_tag="ACHU0J_001774"
     CDS             3715..4569
                     /gene="modD"
                     /locus_tag="ACHU0J_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR1216501.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4595..6549
                     /locus_tag="ACHU0J_001775"
                     /pseudo
     CDS             4595..6549
                     /locus_tag="ACHU0J_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(6599..6853)
                     /locus_tag="ACHU0J_001776"
     CDS             complement(6599..6853)
                     /locus_tag="ACHU0J_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1216502.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7054..7788
                     /locus_tag="ACHU0J_001777"
     CDS             7054..7788
                     /locus_tag="ACHU0J_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1216503.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7790..8401)
                     /gene="emtA"
                     /locus_tag="ACHU0J_001778"
     CDS             complement(7790..8401)
                     /gene="emtA"
                     /locus_tag="ACHU0J_001778"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1216504.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8501..9415
                     /gene="ldcA"
                     /locus_tag="ACHU0J_001779"
     CDS             8501..9415
                     /gene="ldcA"
                     /locus_tag="ACHU0J_001779"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1216505.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9510..11246
                     /locus_tag="ACHU0J_001780"
     CDS             9510..11246
                     /locus_tag="ACHU0J_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1216506.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11857..13271
                     /locus_tag="ACHU0J_001781"
                     /pseudo
     CDS             11857..13271
                     /locus_tag="ACHU0J_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13439..14509)
                     /gene="dadX"
                     /locus_tag="ACHU0J_001782"
     CDS             complement(13439..14509)
                     /gene="dadX"
                     /locus_tag="ACHU0J_001782"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1216507.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14519..15817)
                     /gene="dadA"
                     /locus_tag="ACHU0J_001783"
     CDS             complement(14519..15817)
                     /gene="dadA"
                     /locus_tag="ACHU0J_001783"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1216508.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16147..17679
                     /locus_tag="ACHU0J_001784"
     CDS             16147..17679
                     /locus_tag="ACHU0J_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1216509.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(17731..18450)
                     /gene="fadR"
                     /locus_tag="ACHU0J_001785"
     CDS             complement(17731..18450)
                     /gene="fadR"
                     /locus_tag="ACHU0J_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1216510.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18671..20212
                     /gene="nhaB"
                     /locus_tag="ACHU0J_001786"
     CDS             18671..20212
                     /gene="nhaB"
                     /locus_tag="ACHU0J_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1216511.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20358..20888
                     /gene="dsbB"
                     /locus_tag="ACHU0J_001787"
     CDS             20358..20888
                     /gene="dsbB"
                     /locus_tag="ACHU0J_001787"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1216512.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20934..22202)
                     /gene="umuC"
                     /locus_tag="ACHU0J_001788"
     CDS             complement(20934..22202)
                     /gene="umuC"
                     /locus_tag="ACHU0J_001788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1216513.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22202..22621)
                     /locus_tag="ACHU0J_001789"
     CDS             complement(22202..22621)
                     /locus_tag="ACHU0J_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1216514.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5468 a         6004 c         5719 g         5661 t
ORIGIN      
        1 ttaatgatag agagggtcaa caggcttgcg gaagtcgtca catcttcatg ttttcaatag
       61 ctaaactttg atattgaaca tgatttttat cgatatatat gaaagtatat atcgatttta
      121 cttttcagga tgttcaccat gcccataacc agacggacgt ttgcgcaggc acttgcatct
      181 accctgctcc tgcagtctct accgtccttt tcacaaacag ttaatcgttt tgcttcgcaa
      241 tctcttcccg aagcccaaaa cattacacgc atcgtcagcg caggcgcgcc cgccgatttg
      301 ctcttgctgg ctgtcgcgcc agaaaaaatg gtcggttttt cctcttttga ttttgcccgt
      361 caggcattaa ttccattgcc agagcacatt cgccagttac ccaggctggg acgactcgcc
      421 gggcgcgcca gcacactatc gctggaaggg cttatggcgt tacatcccga tttggtcgtt
      481 gattgcggca atacggatga aaccttgatc tcccaggcac ggcaggttag cgaacagaca
      541 caaatcccct ggttattgct taacggaaaa ctggcgcaat cagcagaaca gttaacaacg
      601 cttggcaaaa cgttaggcga agagcaccgc gccgcagaac aagccaatct cgccagccac
      661 ttcgttggtg aagctcaggc attcgccacc tcacccgccg ctaacctccg cttttatgct
      721 gcgcgcggtc cccgagggct ggaaacgggc ttacagggtt cgttgcatac cgaggcggcg
      781 gaattattag gtttgcacaa cgtcgcgcaa atagccgatc gccacggcct gacacaggtt
      841 tccatggaaa atctcctgcg ctggcagccg gatattattc tggttcagga ggccgttact
      901 gcagatttta ttcgtcgtga tcctctctgg cagggcgtga aagccgttgc ggaacaacgc
      961 atcttatttt taagtggctt gccctttggc tggctcgatg ccccgccggg aatcaaccgt
     1021 cttctgggat tacgcagact tcacgcctgg ctggatcccg ccatcaatcg ccagtttaaa
     1081 agtgacatgc agcattacgc ccaactgttc tggcattgtt cactcagtga tgccgactat
     1141 caaaaattgg tggcgagcta atgagaatcg taaacggatg tattttgctg ctggcagcta
     1201 tcagtatcac ttttgccgcc gtaagtgggg cttatcatct cgatatgcag caactgctgg
     1261 cgctaattct tcgtcaagaa aatgagtctg tgcaggaaca aattgtcttc tggcaaatcc
     1321 gcgtgccgcg tattcttgct gcgctgttac tgggcgcagc gctggcagga gccggaacca
     1381 cctatcaggg aatgttgcgt aatcccttag tttcaccgga tattttaggc gtctccgccg
     1441 gtgccggact tggtgcctgc gcggcaatcc tgtggggatt acccattgtt tacatccagc
     1501 tgtacgcatt ttgcggcggg ctgatggtgg tcgcgggcgt ctggttgatt acgcgtcggg
     1561 tgacccgcca cgatccgatc ttaaccctgg tgctggtagg cattgcgtta ggaacgttgt
     1621 gcggagcggg gatatcgcta attaaaacgc tggccgatcc ttacacccag ttaccgtcaa
     1681 ttaccttctg gttgctgggg ggactgtcca ccgtgacctt acgcgatctc tgctatgctg
     1741 caccgatcat tctcataggc tccttgcccc tgttttttct ccgctggcgc atgaatttac
     1801 tcacgctttc cgacgacgaa gcccgttcgc tgggactgaa cgtgacgcgt ctgcgctttg
     1861 gattgattgt ctgcgccaca ctaatcaccg ccagcacggt agcaatagcg ggcataattg
     1921 gttggatcgg tctggtcgtt ccgcacattg cccgcttgtt gaccggtcat aaccatcagc
     1981 agctgttgcc tatggcaatg tgtactggcg ctattctgct cctgcttacc gatacgctgg
     2041 cgcgcagcat tggaacaaca gaaatcccac ttggcattct gaccgctttt gttggcgcac
     2101 cgtttttcct gcttttgttg ttacgcggag gtcgccagtg actttgcttg ccgttcgtca
     2161 tgcgtctctg ggttactccc gccatccagt gctacgcgac gtgtccttca ccctgtcgca
     2221 aggaacaatt tgttgtctgc ttggcgcgaa tggttgcggc aaaaccacac tcatgcgctc
     2281 cattcttggc gttataccgt tattaaaagg cgagatccta ctggatagcc tctctgtgca
     2341 gacactcagc catcgccagc gcgcacaggc tattgcctgg gtgccgcagg cgcacgacgg
     2401 tatttttgct ttcagtgtgc tggatatggt gttgatgggc ctggccccca ccataggcgc
     2461 tttttccgtt cccggtaagc aagaacgcct gaaagccacg gagcaactgg aaaagctggg
     2521 cattttgcat ctggcgacac gacgctggaa tacgcttagc ggcggtgaac gtcagctggc
     2581 tttaatcgcc cgtgcactcg tccaacaacc gcgcttgttg ttactggatg agcccgcctc
     2641 cagcctcgat ttcggtcatc agatccaact tctggacacg ctggcgcagc tcaaaaataa
     2701 cggcatgaca atgttaatgt cgactcacca tacgctccat gccaacgcca ttgccgacag
     2761 cattatccaa gtagagccag atggacgagt aacacaaggc ttaccgaccg aacagttaac
     2821 aacgaacaaa ctggcggccc tgtatcgggt ttctgccgac caaattcatc atcacctctc
     2881 tgcaattagc cgctaaggaa caatcatgct cattgatgat attgattttg cagaccttta
     2941 tctgcagcaa ctcaaactgg ctcaccgcac agaaaaaacg cccgatcact gggatcaacg
     3001 ggcggagaaa atggcggaaa attgcgcctc gccaacggac agctacctgc aacaactgat
     3061 cgctaaaatt gacctgcaag gcgctcagac cttattcgat atgggctgcg gaccaggcac
     3121 cgtatcgctg gcgctggctg acaaactcac tacggtttat ggcgttgatt acagtcaggg
     3181 gatgctgaac gttgccgccc gccgcgccgc cgcactgaag gctgacaatg tccactggat
     3241 ccagcgcgcc tgggaagagg actggagtga cctgccacgc tgcgatattg ccgtggcttc
     3301 ccgctccact ctggtggctg atatgcgcca tgcaatgagc aaactgaata atcaggcacg
     3361 gcttcgcgtc tatacaactc atctggtcag cacctcgttc gtctctccag ccattcagcg
     3421 ggcggctggt cgggaagtga tcgaattacc caactatatc tttgcgctca acgtcctgta
     3481 tcagatggga atttatgccc acgttgattt tatccgtgga caaaattgcc aacaggataa
     3541 cagcacctgg gaacgttttg agcagaacgt cagctggagt cttggcgcgc ttaacgacga
     3601 tgagcgtgag cggttatatc actggtatca acaacaagat gctcgtgccc tcgcccctgc
     3661 cagccgcgac tgggcgctca tctggtggga tagcgtaccg caggaggcac tgtgatgatt
     3721 ttcctttctc aggcacaaat cgatgcgtta ctgctggaag atatccaggg aggtgacctg
     3781 accacgcgag cattaaatat tggacaccag cctggctata tagcgttttt tcaccgtcag
     3841 ggcggctgcg tcagcggcgt ttctgtcgcg tgtaagatgt taactacgct gggattaact
     3901 attgatgacg cggtcagcga cggttcacaa gcgaacgcgg gtcaacggct aatccgtgcg
     3961 cagggtaatg ccgcagcact tcatcaggga tggaaggcag tccagaatgt gctggagtgg
     4021 agttgcggtg tttctgatta tctcgctcaa atgctggcat tgcttcgtga acgttatcct
     4081 gacggcaata tcgcctgcac ccgaaaagca attccgggca cccgcttact ggcttcgcag
     4141 gcaattctgg ctgccggagg actaattcat cgtgccggat gtgcggaaac catattattg
     4201 tttgccaacc accgccattt tcttcatgac aatcagggct ggtcaggcgc aatcgctcag
     4261 ttacgtcgcc acgcacccga gaagaaaatt gttgttgaag ccgacacgcc gaaagaggct
     4321 atcgccgcgt tacgcgcaca accagacgta cttcagctcg acaaatttag tccgcaacag
     4381 gcaactgaaa ttgcgcaaat agcgccgtcg ctggctcctc actgcacgct ggctctgacc
     4441 ggcggaatta atctgacgac actcaaaaat tacctcgact gcggcattcg cctttttatt
     4501 acctccgcgc cttattacgc ggcaccagct gacattaaag tcagtctgca acccacagcc
     4561 tctatttaat tctttgttaa acggaattta ttctatgaga ctcaaaaaac gttatctctg
     4621 cacagcatta tcacttgcct ttacccagca ggccgtagcg gctcaggaga gcgacacgct
     4681 gaccgtatgg tccagtccgg tatcatcgac gacgaccacc gttctcgatc aacccaccat
     4741 gaaggccttg gataaacaga atgtcgctca ggcattaagt gtcgtccccg gcgtggtgct
     4801 gcaaaagtca ggcagccgca acgaagaaca ggtaaaagtt cgtggctttg atagtcggca
     4861 ggtgccggtc tatttcgacg gtgtgcccat ttatgtcccc tatgacggca acctcgatct
     4921 ggcgcggatt ctgaccaaca atctgggggc agtcgaagtt tccaaagggt attcgtcgct
     4981 gcttcatggg cctaatcaga tgggcggagc cattaatatc accaccctga agaagcaagt
     5041 ctgggatatc gccagggatg gagccgtagt caggacaatg cctacgatat gcatgcttca
     5101 tttgccgcca gcagcgatct tgggtatttg caagtcagcg gtagccagct aaagcaggat
     5161 tttctcggcc tgccgcatgg tgtaaataat gatattgcag gcaaacacgg caagatgatt
     5221 aattcatcgg ctgatgataa acgcggcatt gtgaagctcg gttttacacc gcgcgaaaac
     5281 gatgaataca cgctgactta cattaagcag gatggtgaaa aagataaccc gccatacagc
     5341 ggaaattgtg gtcaaaaatc acgctactgg cagtggccag agtatgacaa agaaagtttt
     5401 tattatcagg gagcgaccca actaaacgac cgttttaccc tgaaaagtcg gctgtatcgc
     5461 gacacctttg aaaatacgct gatgatgtac aactcgctgg ctgatttgaa aaataaaaaa
     5521 ggcagctaca gccattattc cgattacagc gacggtgccg gattacaact ggcagccgat
     5581 gtgcgtgaaa acgatctgct gtcgtttgcc gttaactgga aagatgacgt acaccgggaa
     5641 aaaggtgcgc cgcacgccgc ttacgatcgc tatgaagatc gtacctggtc gctcgccagt
     5701 gaatatcaat gggctgctgc tgataatgtc gatgtcgtgg cgggaatcag ctatgactgg
     5761 cgcgatagcg tagaagcgaa aaaacacgag aaagatggca gcatcaccca ttatgacgac
     5821 aacaatcagt cagcttttaa ctggcaggtg atggggaaat accactttgc caatgaagac
     5881 actctggcgc tttcgtacta tgaccgcaca cgctttccga cgctgaaaga acgctatacc
     5941 acatccaaac ctgcgtataa ccagatagcg attgttaacc cgcagctcaa gccggaacgc
     6001 gctcgcgggg tggatttaac ctggaatggt gccttcacgc gcgactgggg atttgaggtc
     6061 agcgtttact ataaccgggt gagtgatgcc atcctctcgc acaatatcaa tgccgatacc
     6121 attcaaaatc agaacagcgg cacggtggat tacagcggtc tggatgccgg tattaagggg
     6181 aaaatcagca atatactgga tgtaggattg agctacgctc tgatccacgc tgacgccaaa
     6241 cgcaaagaca tcggcaagat aaccgatctt ccaacgcaga caatgaccgc atggatgaca
     6301 ctcaaaccat gggagccgtt aagcgtaacg ctgtcggaag aggcgcgttc ctccagctac
     6361 agcaacagtg acggttcaca aaaagccgcc ggttttgcgg tgacccacat tcgagccgat
     6421 tacaccttag gtcatggctt cagcgtcaat gcgtcggtca ataacctgtt tgatacccaa
     6481 tatgcctaca gtgaagggtt tattgaagaa ggtcgcaatt tctgggcagg tgtcgagtat
     6541 acgttctgat gtgaaagcgg atagcggcat ctgtatcgct atccgcctta tgaaaatctt
     6601 attctcttcg caggaggcgg aatacgccca gcaccaggat agcgcccacc accgccacca
     6661 ggaagctgtg caaattaaaa ccgctgatgg agccgtcaat gccaaacatg gtcgccagcc
     6721 agccgccgac caccgcaccg actatcccga gaatacaggt caggaaaaat ccaccaccat
     6781 cacgccccgg catgattagc ttgccgataa tgccggctat caggccaaaa ataatccagg
     6841 caataattcc catgttcagg ccctcgtttc gatgaatcgg tcgcaagaaa tgcttacgtc
     6901 aacgctctaa gtataggcaa gggcgggatc gatgctgggc gtttgtcggc tgatttaaga
     6961 acaacatgta aaaaatattc acgcatttgt attaagtttt gttaactatg accgataaac
     7021 caaagacagt ttgtcagtca ggagtttttc cgcgtgagtc attaccatga gcagttcctg
     7081 aaacaaaatc cgttagccgt cctgggcgtg ttacgcgatt tgcacaaagc cgcaattcct
     7141 ttgcgtctca gttggaatgg cgggcagctg atcagcaaaa tactggcaat aaccccggat
     7201 aaactggtgc tggatttcgg cagtcaagcc gaagacaaca tcgccgtgct aaaggcacag
     7261 cacattacca ttaccgccga aactcagggt gcgaaagtcg agtttactgt tgaacaacta
     7321 cagcagagtg aatacttgca gcttccggca tttattaccg taccgcctcc caccttatgg
     7381 tttgtacaac gacgccgata tttccgcatc tccgccccac tccatccgcc ttatttttgc
     7441 cagaccaaac tggcggataa cagtacgtta cgtttccgct tgtatgattt gtcgttaggc
     7501 ggcatgggcg cattactgga aacagcaaag cctgccgaat tacaagaagg tatgcgcttc
     7561 gctcaaattg aagtcaacat ggggcaatgg ggtgtttttc actttgacgc ccagttaatc
     7621 tccaccagcg agcgcaaagt gattgatggc aagaatgaaa ccatcaccac tccccgtctg
     7681 agcttccgtt ttcttaacgt cagcccgacg gtggagcggc aattacagcg gattattttc
     7741 tctctcgagc gagaagcccg ggaaaaagcg gacaaagtgc gcgactgatt tacatcgcgt
     7801 ccagtgcctg ctcaagtttg tagatatagc gcggagcctg cggcgcagga tgatttcgcg
     7861 ctacgtgatc gaggaactcg tcagcatcca aatcgttgat tttgctgatc gcctttttcc
     7921 gatctgacga gaaagtccgt agcagcgcac ctgccccgtt agcgtatgac accaccagcg
     7981 catattgcag taccttcgga tcttcaatgc ctgccagcgg gccggtttcc agaatattca
     8041 ggtaagccgc ccccattgaa atattacgct ctggattttt cagctcgctg gtcgtcggct
     8101 caccactcca gcccatacgg cgataaacat cacgtccgga ggttgaagct tttaactgca
     8161 tcaaaccaat ggcattcgat ttactcaccg cgttcggatt accacccgat tcgatagcga
     8221 taatcgccgt gatcaattgt ggatcgacgc cccaggctgc accggctttc tggcttattg
     8281 gcatccactg catcgcacgt tgcaccggaa ctttcgcgtt ccacggcggg ttcgtatagt
     8341 catgctttga tgaacaaccc gctaataaca caatcaaaaa ggcaaaccat ctcaatttca
     8401 cttcttctat ccttatagcc tggaatcgcc gttgaggtga caacggcaca cctaagcggc
     8461 atgatataca tttcgtttct gttgtcagca aggaattgcc atgtctctgt ttcacttaat
     8521 tgccccatcg ggttacagca ttaaacagca cgccgcgctg cgtggtatcc agcgccttac
     8581 cgacgcgggg catcaagtca ataacgtcga ggttattgcc cgtcgttgtg agcgttttgc
     8641 gggtacggaa acggagcgtc tggaagatct caattccctt gccagactga ctacccccaa
     8701 caccatcgtg ctggctgtac gcggcggtta cggtgccagt cgtttactgg cagatattga
     8761 ctggcaggca ctggtggctc gccaacaaca tgacccgtta ctcatttgcg gacatagcga
     8821 ttttaccgcc attcagtgcg gtcttctggc gcaaggcaat gtcatcacct ttagcggccc
     8881 catgctggta gcaaactttg gtgcggatga actgaacgcc tttaccgagc accatttctg
     8941 gctggcgtta cgcaatgaaa ccttcaccat tgaatggcaa ggggaaggcc cgacatgtca
     9001 gaccgaaggc acgttgtggg gaggcaatct tgcgatgctg atttcactga ttggtacacc
     9061 gtggatgcca aaaattgaga acggtattct ggtgctggaa gatattaacg tgcacccttt
     9121 ccgcgtcgaa cgtatgctgt tgcagctcta tcatgcggga attttgccac gtcagaaggc
     9181 gattattctc ggtagcttta gcggcagcac gcccaatgat tatgacgcag gttacaacct
     9241 ggagtcagtg tacgcgtttt tgcgttcccg cctgtcgatt ccactcatta ccggcctcga
     9301 ttttggtcat gaacaacgca cggttacgct gccgttgggc gcacatgcca ttctgaataa
     9361 cacccgggaa ggcgctcagt tgacgattag cggtcatcct gttcttaaaa tgtaaaaaaa
     9421 tcacgcttca gggctaagta aaacgctgtc tctgccgcta atatggtagg gtttatataa
     9481 gagacagcgt aatcaggagt aaccgaccat tggatgccac aacaataatt agccttttca
     9541 tcttaggatc catccttgtc accagcagta tattacttag ttcattttct tcccgtcttg
     9601 gcattcctat tctggttatt tttctggcga tcggcatgct ggcgggagtc gatggcgtcg
     9661 gcggtatccc gtttgataat taccccttcg cctacatggt aagtaacctg gcgctggcga
     9721 ttattttgct cgacggcggg atgcgcactc aggccagttc ctttcgtgtg gcgttaggac
     9781 cggcgctgtc gctggcgacg ctgggcgtac ttatcacctc tggtttaacc ggcatgatgg
     9841 cggcgtggct gtttaatctt gatttgattg aaggcttatt aatcggcgct atcgtcggct
     9901 ccaccgatgc tgcagcggtc ttttctctgc tgggtggtaa ggggcttaac gaacgtgttg
     9961 gctcgacgct ggaaattgaa tccggcagta atgatccaat ggcggtcttt ctgacgatta
    10021 ccctgattgc aatgatccag cagcatgaaa gcagtgtcag ttggatgttc gtggtcgata
    10081 ttctgcagca atttggcctc gggattgtca tcggtcttgg cggtggttat ttactgctgc
    10141 aaatgattaa tcgcatcgcc ctgcctgccg gattatatcc attgctggca ttaagcggcg
    10201 gtattttaat ttttgcttta accacggcgc tggaaggcag cggtattctg gctgtttatc
    10261 tgtgcggttt tctgctgggt aatcggccga ttcgcaaccg ctacggcatc ctgcaaaatt
    10321 tcgacggcct cgcctggctg gcgcaaatcg ccatgttcct ggtgctgggg ctattggtta
    10381 acccaagcga tctgctgcct attgccattc cggcgctcat tttgtcggca tggatgatat
    10441 tcttcgcccg tcccctttcg gtatttgctg gattgctccc tttccgcggt ttcaatctgc
    10501 gtgagcgcgt gtttatcagc tgggtgggat tacgcggcgc ggtgccaatc atcctagccg
    10561 tgttcccgat gatggcgggg ctggagaatg cgcgactgtt ctttaatgtc gccttctttg
    10621 tggttctggt ttcactgctc ttgcagggaa catcactctc gtgggcggcg aaaaaagcca
    10681 aagtggtggt tccgcccgtc ggacgtccgg tgtcacgcgt tggcctggat attcatccgg
    10741 aaaatccgtg ggagcagttt gtttatcaat tgagtgctga taaatggtgt gtaggtgcgg
    10801 cactgcgcga tttgcatatg ccaaaagaga cgcgtattgc agccctgttt cgtgataacc
    10861 agttgcttca tcctaccggc agcacccgac tgcgcgaagg cgatgtattg tgtgtaattg
    10921 gtcgggaacg cgatctcccg gcgctcggta aactgttcag ccagtcgcca ccggtcgcgc
    10981 tggatcaacg cttctttggt gacttcattc tcgaagccag cgccaaatat gctgatgttg
    11041 cgctgatata tggcctggaa gacgggcgag aatatcgtga taaacagcaa acgctgggtg
    11101 aaattgtcca gcagttgcta ggcgcggcac cggttgtcgg tgaccaggta gagtttgccg
    11161 ggatgatctg gacggtggcc gagaaagaag ataatgaagt gttgaagatt ggcgttcggg
    11221 tggctgaaga ggaagctgaa tcttaaattt aatgcagcat ctatcgccgg atgcgatgct
    11281 ttactgaacg cagcctgagc ctgtaataaa cttgtcattt cctgattttg atatgctcat
    11341 cccaacatca gaagaaggtt aaattatgga tgacaaacca ttgcaggctc tggctaatga
    11401 actcaaaaca ccggctcaag aaagtcagcg taagggcttt ttccgactgt aacaggtaat
    11461 aaaaaccagc cccgcattac cggtcaacca tcaggggccc ctgcctgaca tcgcctgaac
    11521 atattccccc acgcaacctg ccgcactgac atagcccaca caataggcgt ttcatccgcc
    11581 tgtagcaccg gatgcatgag cgatttctta tagttattcc tttgcttaat aacgattatt
    11641 tccgggtcgt tcactctcgt attttcttta atacgtcaga gctacctagg ccgtaataca
    11701 gcatgtacca gacccgacac ggcatttctg accaggaaaa aagtcaacga tgagtagtga
    11761 tatcagcgca aatgacgtcc tgtggctcct ttgcggtgac cacattcacg tctccggcgc
    11821 tgccagagga tatttatttc aaggaaacgt agcattatgg ctagctttaa agacatctgc
    11881 ccattcgccc ccgccattcc cccatcttcc gttaacccag gcgtacgacg cccgccaggg
    11941 cggctcttct caagaatcaa attttgcaga acatgaagta tctcagcccg caaaaattca
    12001 gctggggtga tgccccctgg cagatcattg acctcagtat cgcgggcaaa gtgaacatac
    12061 aggtggacaa caataccatc atcacgttgg ggactcgtct gaatcagcaa cataacgagt
    12121 tcatgatggt cgccaaatgg tgcgaatggg ctatacagca ggatggtttg caagaaaacc
    12181 tgcaaaaaaa cctgtacgaa atcctggaag aaaaccagca aaacaaacag tcagaaattc
    12241 cccaagaaga cctgaaagaa agtctggaag aaatcaagga aaatatcctg gaagaaaacc
    12301 tgccagcaag ccggattgaa aaccgggcag aagcattgcg cagaatgaag gaatgtctca
    12361 ttacaagacg gagtatgctc aatcttagca acctgggatt gacttcactc cctgagaatt
    12421 tgcctccaca tctgattgaa ttttactgca gtaaaaacgt gttgaccgcg ttaccgaagg
    12481 tgatgccaaa gtggctgctg gtgcttgact gtacagataa cgtcttgatc ttgttaccga
    12541 aggtgcagcc ctcaaaactg atggtactga agtgctatga aaacagtatt atctggctgc
    12601 ctgagctgtc gactaacctg agagtgatta actgttctga aaacttcttg caatttttac
    12661 cgccgtcgat gccccagtac ctgtataaac tatcctgtgc cggcaacaat attaactcaa
    12721 tacctgatga gatgctggag aacttgactc gcctgaaagt atttgactgt tctagtaacg
    12781 atttgatctc ttcaccacgg ctgccgccca aactaataat ttactactgt ggagaaaaca
    12841 agtttaaaac ggtacaggtg ccgcagcccc agagcctgaa ggtgtttgac tgtaatggta
    12901 acccgtggga caaagacaat ttaccgacgc tgctcaaagc cgtcgagggc ctgaaaaaac
    12961 agcagggcct gaaggacctt ttggactttt tgcacaagga aggctaggtt gacctggaag
    13021 gactcgagga gctggaggac cttatggatc tggagttcct ggatgacccg gaactcctgg
    13081 agcgcgtgaa ggtacaggag gacctggagc tcctggatca acagttgggc ctgttgagtc
    13141 tggaaagaca acaggactcg cagcctgtta atcaacaatc tgaacatgaa cccgaatctg
    13201 catcaagggt gaagcgtgat ttatctgaag tcgactccga gtcaacaatg aagcgtaagc
    13261 gttttatgta gaacgcctgt gcggcgagtt caggttccgg ggaaatagtg tccattcttt
    13321 taatggacac tatcatgggc aatggctctg tcacattaac aactacagtt gctgaccagc
    13381 cggatggatg attttttcgc aaccagaaga cgctgcctcc gatccggctt acaacaagtt
    13441 acactgtcac aaccgggacg cgtaacgcca gtgcgcacat caactcatag cccaccgttc
    13501 cggcagcggc ggcgacatca tcaattttga tctccttgcc ccacagctca accggcgtac
    13561 caatccccgc ctgcgggcaa ggcgttaaat caaccgccag catatccatc gagacggtcc
    13621 ccaccgtcat ggtgcgcacg ccgtccacta aaacaggagc accggtaggc gcatggcgcg
    13681 gataaccgtc ggcgtatcct gcggcgacaa tgccgattcg ctgttcatcg cgcgcagtat
    13741 agcgaccgcc gtagcccaca cgctcgcccg cttttagcgt ctggacacca ataatctcac
    13801 tgcttagcgt catcaccgga cgtaatccgg tattggcgat atcacgccac tgaccggacg
    13861 gcgaagcgcc atacaaaata atgccaggcc gaacccagtc aaaatgcgct tccgggtgcc
    13921 acagagtcgc cgccgaattg gacaacgaac gccgacactc cagcccctcc gccgcctgct
    13981 caatacgcgc catcgcgccg gaaattccat caggatgttc cgcttcagca aaatgcgaca
    14041 tcagggtcat ttcgccaaca ttcgccattg cccgcaactg ctgccagacg gtaagcacgc
    14101 gatcggactg gaagcccaac cgattcatcc cactgttcac tttaagataa atatccaacg
    14161 gtgcttttag ccgcgcattt tgcagtgctt tgagctgcca gttgctgtgt acgcaggtgg
    14221 tcaggcggtg ctggtcataa atctccagat cctgagcatg gaaaaatcct tccagcatca
    14281 ggatcggccc cttccagccg cgctcacgta acgttattgc ctcttccagg ttaagtaatg
    14341 caaagccatc ggtggccccg agcgcgctcc agatacgctc aataccatgc ccgtaagcgt
    14401 tcgcttttac caccgaccag acgcgcgcgt gcggcgcggc ctggcggaca atggacagat
    14461 tctgttttaa tgcctgcaga tcgaggctgg cctgtatcgg acgggtcatc tcgtttcctt
    14521 agctgtgtgc gccatgtaaa tggcccggac gtgatggcgt aaatccacgg ctgtagcgcg
    14581 ctacgcttag atcctcatat gggatcgctg gcgtgcgacc agagagcaga tcgcttaaca
    14641 actgaccgga accgcaagcc atcgtccagc cgagcgtgcc gtgaccggta ttcagccaca
    14701 gatttttaaa gcgtgtactc ccgacaaccg gcgtaccgtc tggcgtcatc gggcgcaaac
    14761 ccgtccagaa agtcgcctgc tcgacatgac cgccgcgtgg atagagatcg cgaaccacca
    14821 tttccagcgt ttcacgacgc ggttgcaaca gctcggtgtt aaaaccaaca atttccgcca
    14881 ttccgccaac acgaatgcgg ttatcgaaac gggtaatggc gattttgtag gtttcatcaa
    14941 gaatggtgga tactggcgca ccatcttcct gcgcaattgg aatagtcagc gagtagcctt
    15001 tcagcggata caccggaata tcaacaatgc ctttgagcat cgccgtcgag taagaaccaa
    15061 acgccatcac atacgcatcg gccttaatca cttcatcgcc aaacttcacg ccgtagattt
    15121 gctcgccgtc gcaaagcagt tggtcaacgg gcgtattaaa gcggaattta acccccgcct
    15181 gctctgccat ccgcgccaga ttctgggtaa acagctggca gtctccggtt tcatcattgg
    15241 gtaactgcag gccgcccgtc agtttgtgcg ctacttctgc cagcgcggga tccacttccg
    15301 ccaggcggct ggattccagc agctgatacg gtacgccggc atcttccagc acggcgatat
    15361 cgcgggtcgc attttcatac tgttgttcgg tacggaacag ttgcagcgtc ccaccctgac
    15421 gcccttcata ctgaatattg gtttcggcgc gcaatgcttt caggcaatca cggctgtatt
    15481 ccgccagacg caccatccgc cctttgtttt ccatgtagtg gctggtgtcg cagttacgta
    15541 acatttgcca catccatttc aactgaaact gcgtaccgtc gagacgaacc gccagcggcg
    15601 catggcgctg gaacatccat ttaatcgctt ttaaaggcac acctggtgct gcccacggtg
    15661 cagcatatcc gggggagatt tgcccggcat tcgcggcact ggtttccagg gcggcccccg
    15721 gctcccgatc aatgacggtg acctcatgtc ctgcctgatt taagtaccag gcgctggcaa
    15781 cgcctaccac accacttccc agtatgacaa ctcgcatagc cactccgata acggtagata
    15841 cagtaaaaga ataataatct aattacatct tgataactca gttgaaaata ttattcaaca
    15901 tacggctttt ttatggtgag ctgactcaca tctccctgac tctgcggcaa tatggcgtct
    15961 ttagtatctc ctgctgaacg ccctttttat ccagcataaa attccgttca gaagcggatt
    16021 agtggcactc tgataataag aaatcgcaaa gaaaaaaatt ctccggcgac gcggttttta
    16081 acaggtgttc tatgcttgaa atgaggtgct ccacacagag agcgccgaca acaatgaggt
    16141 gcgcgtatgg cgacgatcga ttctatgaat aaggacacca cacgtttgag cgatggaccc
    16201 gactggacgt tcgacctgct ggatgtttat ctggcagaga tagaccgggt ggcgaaactc
    16261 taccggctgg atacctaccc gcaccagatt gaagtgataa cctcagaaca gatgatggat
    16321 gcctattcca gcgtcggcat gccaattaac tatccgcact ggtcattcgg taaaaagttt
    16381 atcgagactg aacggctgta taagcacggt cagcaagggc tggcctatga aatcgtcatt
    16441 aactctaacc cgtgtatcgc ttacttgatg gaagagaaca ccattactat gcaggcgctg
    16501 gtgatggcac acgcctgcta tggacataac tctttcttta aaaacaattg cttattccgc
    16561 agttggaccg acgccagttc gattgtcgat tatctgattt ttgcccgtaa atatattacc
    16621 gagtgcgaag aacgttatgg cgttgatgaa gtagaacggc ttctggactc gtgccacgcg
    16681 ctgatgaact acggcgtgga ccggtacaaa cgcccgcaaa aaatctcgct gcaagaagag
    16741 aaagcccggc agaaaagtcg cgaagagtat ctacaaagtc aggtcaatat gctctggcgt
    16801 accctgccga agcgcgagga agagaaaacg gttgctgaag cgcgccgcta tccgtccgaa
    16861 ccacaagaaa acctgctcta ttttatggag aaaaatgcgc cactgctgga atcatggcag
    16921 cgtgaaatcc tgcgtattgt gcgtaaggtg agccagtatt tttatccgca aaaacagact
    16981 caggtgatga acgaaggctg tgcgaccttc tggcactata ccatccttaa ccatctgtat
    17041 gatgaaggga aagtaacgga acgttttatg ctggagtttt tgcacagcca caccaatgtg
    17101 gtcttccagc ccccctataa cagcccgtgg tacagcggca tcaacccgta tgccctcggg
    17161 ttcgccatgt tccaggatat taaacggatt tgtcagtcgc caacggaaga agacaaatac
    17221 tggttcccgg atatcgccgg ttccgactgg ctggaaacgc tgcatttcgc gatgcgtgat
    17281 ttcaaagatg agagttttat cagccagttt ctgtcaccga aagtgatgcg tgatttccgc
    17341 ttcttcaccg tgctggatga tgatcggcat aattatctgg agatttccgc tattcataat
    17401 gaagaaggtt atcgggagat ccgtaaccgg ttatcgtcgc aatataactt aagtaatctg
    17461 gagccgaata ttcagatctg gaacgtggat ttgcgcggcg accgttcgct gacgctgcgt
    17521 tatattccac ataatcgcgc accgctggat cgggggcgca aagaagtcct gaagcatgtg
    17581 catcgcctgt ggggatttga tgtgatgctc gaacagcaaa acgaagacgg cagcatcgag
    17641 ttgctggaac gttgcccgcc aagaatggga aatctgtaaa aacaacaaaa aacccctcgt
    17701 ttgagggggt tgctctttaa acggaaggga ttatcgcccc tgaatggcta aatcacccgg
    17761 cagatttttc tgcatccggt gccaaatctc gccactctca tgcccatagc gacgcactgt
    17821 ttcgtacacc tgatcgtgcg cgccttcact gcacaacgcc gacagtttgt ggtagaagcc
    17881 cagcgccaga ctgcgcgctt ccggattggc gaaatagtga cgaccaatgc gcgtatacag
    17941 ccctttcatc ccgttaagga tcagaccgta aatcgggttg ccggaagcaa acgccaggcc
    18001 gcggaatatg ttgtaatcca gctcggcaaa ggcatcggcg tgatcggcca cttcattagc
    18061 ggtagccagc acttcctgcg ctttatcggg atgctgacga aacgcggtgc gaataaaaat
    18121 agtggaaata ttggtacgca ccgacagcaa attatcaata agctgcggca cactttcgtg
    18181 atccagtcgc gccagtgttt caaggatatt taaaccggaa gtttcccaga aattattcac
    18241 cttcgtcggc ttgccatgtt gaatggtcaa ccagccatct cgtgccagac gctgtaacac
    18301 ttcacgtaac gtagtacgcg ttacgccaat taattctgaa agttcacgtt ctgcgggcaa
    18361 aatagtcccg ggagggaagc ggttattcca gatactttca ataatgtact cttccgcgaa
    18421 acccgccggg ctttgcgcct taatgaccat agtgagattt ccataacaca gcaaaacaaa
    18481 gttggactca tcataccaga gggctaacta cgctgatagc agacaaaaag aagaaaaagg
    18541 gatcaaggct tcattttccg catctctttc cccttgacta tcaagacttg cctcactcct
    18601 gcctgaccgt taagctttgc gtttaaacat aaaaacatca ttgattattt gtaaggtaag
    18661 ggaaaccatc atggagatct cctggggccg cgcgctatgg cgcaattttt tgggccagtc
    18721 ccccgactgg tacaaactcg ccctcatcat tttcttaatc gtaaacccgt taattttcct
    18781 catcagccct ttcgtcgcgg gctggttgct ggtagcggaa tttattttca ctctggcgat
    18841 ggccctgaaa tgctacccgc tactccccgg tggtctgttg gctatcgaag cggtattcat
    18901 cggcatgacc agcgcggaac acgtccgtga agaggtggcg gcaaatcttg aagtcttgct
    18961 gttattgatg tttatggtgg cgggtatcta ttttatgaaa cagctgttgc tgttcatatt
    19021 tacccgtttg ctgttaagca ttcgctccaa aatgctgctg tcgctctctt tttgcgtggc
    19081 ggcagcgttc ctctccgcgt tcctcgatgc cttaaccgtc gtggcggtgg tgatcagcgt
    19141 tgcagtcggt ttttatggta tttatcatcg cgtagcctct tcccgtaccg aagacaccga
    19201 cctgcaagac gatagtcata tcgacaagca ttacaacgtg gttctggaac agttccgtgg
    19261 ctttctgcgt agcctgatga tgcatgccgg tgtcggcacc gcattaggcg gcgtaatgac
    19321 catggtgggc gaaccacaga acctgatcat cgctaaagcg gctggctggc attttggcga
    19381 tttcttcctg cgcatgtcgc cggtgaccgt tccggttctg atttgtggcc tgttaacctg
    19441 cctgctggta gagaagctgc gttggtttgg ctacggtgaa acgctgccgg agaaagtccg
    19501 cgaagtgttg caacagtttg acgatcaaag ccgccaccag cgtacccgtc aggataaaat
    19561 ccgtctgatt gtccaggcga ttattggcgt ctggctggtg actgcgctgg cgttgcatct
    19621 ggcggaagtt ggcttgattg gtttgtcagt cattattctg gcaacatcat tgaccggtgt
    19681 caccgatgaa catgctatcg gtaaagcctt caccgaatct ctgccattca ccgcactgtt
    19741 gacggtgttt ttctcggtag tcgcggtgat tatcgaccaa caactgtttt cgccgattat
    19801 tcagtttgtg ttgcaggcat ctgaacatgc tcagctgtcg ctgttctata ttttcaacgg
    19861 tctgctgtca tccatttcgg ataacgtctt cgtggggacg atttatatca acgaagcgaa
    19921 agcggcaatg gaaagtggcg ctatcacgtt gaagcaatac gagctgctgg cggtcgccat
    19981 taataccggt accaatctgc cctctgtcgc tacgccgaac ggtcaggctg cgttcctgtt
    20041 cctgctgacc tctgcactcg cgccattgat tcgcctctct tatggccgca tggtgtggat
    20101 ggccctgcct tacaccctcg tactgacact cgtcggcttg ctctgcgtcg agtttacgct
    20161 tgcccctgta accgaatggt ttatgcaaat gggctggata gcaacgcttt gataacaact
    20221 taccgggcat ataaatgccc ggtttgcctt ttcgcccgat aattgtccaa ttgcgtccta
    20281 tttttgctgc ctcctggtgg cgcagcgaat gaattggttt aaactgcgca ctctatgcat
    20341 attgcaggga aatgattatg ttgcgatttt tgaaccaatg ttcacaaggc cggggcgcgt
    20401 ggctgttgat ggcgtttact gctctggcac tggaactgac ggcgctgtgg ttccagcatg
    20461 tgatgttact gaaaccttgc gtgctctgta tttatgaacg ctgcgcgtta ttcggcgttc
    20521 tgggtgctgc gctgattggc gcgatcgccc cgaaaactcc gctgcgttat gtagcgatgg
    20581 ttatctggtt gtatagtgcg ttccgcggtg tgcagttaac ttacgagcac accatgcttc
    20641 agctctatcc ttcgccattt gccacctgtg attttatggt tcgtttcccg gaatggctgc
    20701 cgctggataa atgggtgccg caagtgtttg tcgcctctgg cgattgcgcc gagcgtcagt
    20761 gggatttttt aggtatggaa atgccgcagt ggctgctcgg tatttttatc gcttacctga
    20821 ttgtcgcggt gctggtggtg atttcccagc cgtttaaagc gaaaaaacgt gatctgttcg
    20881 gtcgctaatc cattcggcgc tcctgcggga gcgctttttt cctgccgcta tatttatttg
    20941 acccgcagta aatcagaact tcgcgttgta taacgtggtg aaagcatagc tcgcttcatc
    21001 tgccattgtt gttggatccc ctgcccggca aaatagagtg ttcctctgcc ctctttagca
    21061 ttcagagtat ccattaccgc catcaattgc tcactcccgg ggcgcggtgc gttgtcatcg
    21121 aataaattga gctgcgcgac tccctgactg aagaaatccc ccagcatcac gcccgctttt
    21181 tggtagcgat ggcccgcttg ccagatggca tccagagatc gcgtagcagc gttaatgata
    21241 tccctgctgt cctgagtggg cgtcagcagt tttaccgacg cgctattgcc gtaataaggt
    21301 tcattgagcg caaatggtga cgtcttaata aatgtggaga taaaccgaca atattgatgc
    21361 tcactgcgaa gtttttccgc cgcccgggca gcgtaactac aaatggcctg ccgcatcgac
    21421 gtataatccg tgatgcgttc accaaacgag cgggaacaga taatttcctg cttcgtcggt
    21481 gcaaactctt ccagttgcaa acagggttcg ccgcgcagtt cacgcaccgt tctttcgagc
    21541 atgacattaa aatgtttacg gataaaccgg atatctgtat ccgccaaatc gagaacggtt
    21601 ttgatcccca tcgcgtccag ttttttgctg atccgccgtc caatccccca gacgtcatcc
    21661 acggggagag cagacattaa tttacgctgg cgttccagat ttgataaatc caccacccca
    21721 cccgtctgcc gctgccattt ttttgccgca tgattggcaa gcttagccaa cgttttggtc
    21781 tgggcgatcc ccacaccaac agtaagatgg gtacgttgta gcaccgttgc gcgaatttct
    21841 ctgccaaaat cagtcagatc gcgacaatta cgcacacctg tcagatcgca gaatgcttca
    21901 tcaatactgt aaatctcgac gcggggcgat agttcttcca gcgtcgacat cacccgattg
    21961 ctcatgtctg cgtaaagctc ataattgctg ctaaagcaaa ccacaccaca gcgacgaaac
    22021 agatcttttt gtttgaacca gggatcgccc attttaacgc ccagcgcctt tgcctcagcg
    22081 tttcgggcga taacgcaacc gtcattattc gatagcacaa ccaccggttt accccataaa
    22141 tcagggcgaa acaccgtctc acagctggca taaaacgcgt ttacatcaca gagggcaaac
    22201 atcagcgcat cgccttaacg acgtggatca ccacaccaaa gacatccagc gtatcttcgc
    22261 tactgatggt aatgggcgag tacgcactgt tcatgggaat aagctgtacc gtcgggcgta
    22321 gttgcaattt tttcaccgta aactcgccgt caacagcagc gatgacaata tcaccatggc
    22381 tggcggtaat agcactatcg acaatcagta aatcaccgtc actaattcca ccatcaatca
    22441 tagaatcacc acttgctttg acgaagtaag tcgcgctggg atgctggatc aacagttgat
    22501 tcagatcgat gcgctgttca acgtaatctg ctgccggtga aggaaagcca cactgaacaa
    22561 gatcgctaaa tagcggaaaa gtcacaattt cgcggagatc cgcaggcttg ataaacaaca
    22621 taataatctg cctgaagtta tactgttttt atatacagtg gtctgttctt gccagcagat
    22681 caatactgat tcaggctatc aatatttgtc gctgcataga ctgctgattt ttcgttctct
    22741 tatcctgtgc tcacgtggcc ttctggcgac gacgctcatc cagcagaaat gaaaaatatc
    22801 acccggcaaa aaaatagaat agaagcattc gccataatga cattaaacat tg
//
LOCUS       DAZPDE010000050        22433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPDE010000050 DAZPDE010000000
VERSION     DAZPDE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.050"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHU0J_001790"
     CDS             82..2682
                     /locus_tag="ACHU0J_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR1216515.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHU0J_001791"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHU0J_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHU0J_001792"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHU0J_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHU0J_001793"
     CDS             4302..4817
                     /locus_tag="ACHU0J_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1216516.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHU0J_001794"
     CDS             4783..5520
                     /locus_tag="ACHU0J_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1216517.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHU0J_001795"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHU0J_001795"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1216518.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHU0J_001796"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHU0J_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1216519.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHU0J_001797"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHU0J_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1216520.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHU0J_001798"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHU0J_001798"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1216521.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHU0J_001799"
     CDS             10827..12734
                     /locus_tag="ACHU0J_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1216522.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHU0J_001800"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHU0J_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1216523.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHU0J_001801"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHU0J_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1216524.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHU0J_001802"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHU0J_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1216525.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHU0J_001803"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHU0J_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1216526.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHU0J_001804"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHU0J_001804"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1216527.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHU0J_001805"
     CDS             complement(17402..19162)
                     /locus_tag="ACHU0J_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1216528.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHU0J_001806"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHU0J_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1216529.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHU0J_001807"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHU0J_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1216530.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHU0J_001808"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHU0J_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1216531.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..22415
                     /gene="sxy"
                     /locus_tag="ACHU0J_001809"
                     /pseudo
     CDS             22005..22415
                     /gene="sxy"
                     /locus_tag="ACHU0J_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5725 a         5265 c         5879 g         5564 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tgggtagact ggccccctga atc
//
LOCUS       DAZPDE010000051        22149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPDE010000051 DAZPDE010000000
VERSION     DAZPDE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.051"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(498..1589)
                     /gene="ychF"
                     /locus_tag="ACHU0J_001810"
     CDS             complement(498..1589)
                     /gene="ychF"
                     /locus_tag="ACHU0J_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1216532.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1706..2290)
                     /gene="pth"
                     /locus_tag="ACHU0J_001811"
     CDS             complement(1706..2290)
                     /gene="pth"
                     /locus_tag="ACHU0J_001811"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1216533.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2568..2846
                     /gene="ychH"
                     /locus_tag="ACHU0J_001812"
     CDS             2568..2846
                     /gene="ychH"
                     /locus_tag="ACHU0J_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1216534.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2901..4580)
                     /gene="dauA"
                     /locus_tag="ACHU0J_001813"
     CDS             complement(2901..4580)
                     /gene="dauA"
                     /locus_tag="ACHU0J_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1216535.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4705..5652)
                     /gene="prs"
                     /locus_tag="ACHU0J_001814"
     CDS             complement(4705..5652)
                     /gene="prs"
                     /locus_tag="ACHU0J_001814"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1216536.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5803..6654)
                     /gene="ispE"
                     /locus_tag="ACHU0J_001815"
     CDS             complement(5803..6654)
                     /gene="ispE"
                     /locus_tag="ACHU0J_001815"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1216537.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6654..7277)
                     /gene="lolB"
                     /locus_tag="ACHU0J_001816"
     CDS             complement(6654..7277)
                     /gene="lolB"
                     /locus_tag="ACHU0J_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1216538.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7491..8747
                     /gene="hemA"
                     /locus_tag="ACHU0J_001817"
     CDS             7491..8747
                     /gene="hemA"
                     /locus_tag="ACHU0J_001817"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1216539.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8789..9871
                     /gene="prfA"
                     /locus_tag="ACHU0J_001818"
     CDS             8789..9871
                     /gene="prfA"
                     /locus_tag="ACHU0J_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1216540.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9871..10704
                     /gene="prmC"
                     /locus_tag="ACHU0J_001819"
     CDS             9871..10704
                     /gene="prmC"
                     /locus_tag="ACHU0J_001819"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1216541.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10701..11093
                     /gene="sirB2"
                     /locus_tag="ACHU0J_001820"
     CDS             10701..11093
                     /gene="sirB2"
                     /locus_tag="ACHU0J_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1216542.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11097..11906
                     /gene="sirB1"
                     /locus_tag="ACHU0J_001821"
     CDS             11097..11906
                     /gene="sirB1"
                     /locus_tag="ACHU0J_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1216543.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11942..12796
                     /gene="kdsA"
                     /locus_tag="ACHU0J_001822"
     CDS             11942..12796
                     /gene="kdsA"
                     /locus_tag="ACHU0J_001822"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1216544.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12945..13052)
                     /locus_tag="ACHU0J_001823"
     CDS             complement(12945..13052)
                     /locus_tag="ACHU0J_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1216545.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(13457..14557)
                     /gene="chaA"
                     /locus_tag="ACHU0J_001824"
     CDS             complement(13457..14557)
                     /gene="chaA"
                     /locus_tag="ACHU0J_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1216546.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14827..15057
                     /gene="chaB"
                     /locus_tag="ACHU0J_001825"
     CDS             14827..15057
                     /gene="chaB"
                     /locus_tag="ACHU0J_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1216547.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15215..15910
                     /gene="chaC"
                     /locus_tag="ACHU0J_001826"
     CDS             15215..15910
                     /gene="chaC"
                     /locus_tag="ACHU0J_001826"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1216548.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(15954..16307)
                     /gene="ychN"
                     /locus_tag="ACHU0J_001827"
     CDS             complement(15954..16307)
                     /gene="ychN"
                     /locus_tag="ACHU0J_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1216549.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16492..17886
                     /gene="ychO"
                     /locus_tag="ACHU0J_001828"
                     /gene_synonym="iatA"
     CDS             16492..17886
                     /gene="ychO"
                     /locus_tag="ACHU0J_001828"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1216550.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17887..18537)
                     /gene="narL"
                     /locus_tag="ACHU0J_001829"
     CDS             complement(17887..18537)
                     /gene="narL"
                     /locus_tag="ACHU0J_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1216551.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18530..20326)
                     /gene="narX"
                     /locus_tag="ACHU0J_001830"
     CDS             complement(18530..20326)
                     /gene="narX"
                     /locus_tag="ACHU0J_001830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1216552.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20665..22056
                     /gene="narK"
                     /locus_tag="ACHU0J_001831"
     CDS             20665..22056
                     /gene="narK"
                     /locus_tag="ACHU0J_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1216553.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5480 a         5498 c         5715 g         5456 t
ORIGIN      
        1 gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga aaaagcaaaa
       61 aacatcgata ttcatttttg ttcaatggat tttctggcct atcgttatta ttgtgctact
      121 ggttatcatt ttcatcttgc caggatcgca cgtaatcacg ttattcgggg actttatctg
      181 cgggatatta cccttcaggt aagtcctttt ctcgccagca tattggagcg agatcaatat
      241 tgatcagctg tgaagagggc tgatgtgtag tggaacaaaa actgacgtta tatcactgct
      301 aatgagatat cagtcatctg aacacttatg tctttaatta ttctcgtggt tcactgtagg
      361 caataagcac aaaagtgtag gatgttacaa gaatgattag gactcggtga aatgaaaaat
      421 ccacgcaatt gcgtggattt tatatacttt tgcgctcttc atgagattta gcgaaacctc
      481 atgagacaat aaattaatta gacgttgaaa aggaagttca tcacatcgcc atctttcacg
      541 atgtaatctt taccttctgc acgcattttg cctgcttctt tcgcgccttg ttcacctttg
      601 taagtgatga aatcttcaaa cgagatggtt tgtgcacgga taaagccttt ttcaaaatca
      661 gtatggattt tgcccgctgc ctgcggcgcg gttgctccaa ccggaatggt ccatgcacgc
      721 acttctttca ccccagcggt gaagtaagtt tgcaggttca gcagcttata accggcacgg
      781 atcacacggt tcaggcccgg ttcttccagc ccaagctcct gcataaactc gtcacgttct
      841 tcgtcgtcca gttcggcaat gtctgcttca acagcagcac aaaccggaac cacaacagaa
      901 ccttctttcg ccgcgatttc acgcacctgg tcaagatatg ggttgttttc aaaaccatct
      961 tcgttgacgt tggctatgta cattgttggt ttcagcgtca ggaagctcag gtaacgaata
     1021 gccgctttct cttcagcgct taaatccagc gcgcgcagca tacctgcgtt ttccagctgg
     1081 ggcaggcatt tttccaggac cgccagctca gctttcgcgt ctttatcgcc acctttggct
     1141 ttcttctgta cgcgatgaat cgcacgttcg caggtgtcga ggtctgccag cgccagttcg
     1201 gtgttgataa cttcaatatc gtcagccggg ttaactttgc cggaaacgtg aatgatgttg
     1261 tcattttcaa agcagcgaac aacgtgaccg atcgcttcgg tttcacggat gttggtcagg
     1321 aactggttac ccagaccttc gcctttcgat gcgcctttta ccagaccggc gatatcgaca
     1381 aattccatgg tcgtgggaag cgtacgttgc ggttttacga tttcagccag ttgatccagg
     1441 cgagggtcag gcattggtac gacgcctgtg ttcggctcaa tggtgcagaa tggaaagttg
     1501 gccgcttcaa taccggcttt ggtcagcgcg ttgaacaggg tagatttccc gacgttgggc
     1561 aaaccgacga taccgcattt gaatcccatg atttaaatca ccttaatatc ttaataatca
     1621 acctgttatt gctaacagat tgcagaaatg gaaataactt tgcctattat acacggcact
     1681 cggcaaaaat gccgcagaca acgacttatt gcgctttaaa ggcgtgcaat cggttcgttg
     1741 ctttggtcaa gccatctgta aaccacattt cagtacaacg cgccgcttcg tcgatggctt
     1801 catcaattaa cttctgttca ctaacaggcg gtttgcctaa cacaaaaccg acaactttat
     1861 ttttatcgcc cggatgaccg attccgatgc gtaaacggtg aaagttaggg ttattaccca
     1921 atttactgat gatgtctttc agtccattgt gaccaccatg gccaccgccc aacttaaatt
     1981 tggcgacgcc aggaggcaga tccagttcgt cgtgggccac cagaatttcg tccggattaa
     2041 tgcggaaaaa actggccatc gccgcaacgg ctttgccgct gagattcata aatgtagtcg
     2101 ggactaacag gcggacatct tcgcttccaa gggtgactcg cgaagtataa ccaaagaatt
     2161 tagcctcttc gcgcagcgga gcgcgcaaac gctctgccag taagtcaacg aaccaggcac
     2221 cagcattatg tcgcgttgcg gcgtattcag caccggggtt cgccaggccg acaatcaatt
     2281 taatcgtcac gtttttttgt cctgagtgtg tacataactg gcgcgtagtt tactggttgc
     2341 ggccccgctt gacaaaaaac tgcgtatcaa atgcagataa cgtaataatt gcctgagtgg
     2401 actattagaa agtcaaggtg ttcaggcgtt tatttgtaaa gttttgttga aataagggtt
     2461 gtaattgtga ttacgcccgc acataaccca cagggtgttg tctatacttt acacataagg
     2521 aagaggggta ttccctgtta caacccagaa agttccggag gtgacatatg aaacgcaaaa
     2581 acgcttcgat actcggtaac gtgctcatgg ggttgggtct ggtggtaatg gtggtcggcg
     2641 tggggtattc aatcctcaac cagttaccac agtttaatat gccccagtat ttcgcacatg
     2701 gtgcagtgct aagtattttc gtcggtgcca ttctctggct ggcgggtgcc cgtgttggcg
     2761 ggcatgaaca ggtgtgcgac cgttactggt gggttcgtca ctatgacaaa cgttgccgcc
     2821 gtagcgataa tcgccgtcat agctaacaaa taatgcagtt tgctgaccag tcagtttgcg
     2881 ctgactggtc aatctcgtac ttataaatcc gccatcgccg cgcgacgatt cgggaagaac
     2941 gcgaggcgtc ccgggatcgg ttgaatgcct gcgcgagcca tagttcgcag tggctggaat
     3001 tccacgttgc acacgcgcag ttcacatcct tcgggcagac gcttcacaaa acgctggaac
     3061 gcatcaagac caccagcatc aagtaccgga acggcatccc acttcagaat cacaatccgt
     3121 ttgccttcaa gacgtgactc caggtccgtg aataagcctt cagcagcagc aaaaaacagc
     3181 gggccaataa cgcgcagaac caggacatcg tctggaacat ctacgactac cggtgccagg
     3241 cgagtcatac gtgcgatacg acgcataaac agcagcgatg ccagcacgat ccccacgctg
     3301 atggcaataa ccatatcaaa cagcacggtc agtgacatac acagcagcat gacgatgatg
     3361 tcatctttcg gcgcatgacg cagcaagtcg accactttgt gcgcttcact catgttccac
     3421 gccaccatca acagcagggc tgccatggcg gaaagcggca gccaggagag cagtggtgcc
     3481 agtaccagca gggcgagaat aaccagaata gagtggatca ccgccgagat aggggaagtt
     3541 gccccggcac ggacgttagc ggcagaacgc gcgatggcag cagtagcggt aataccacca
     3601 aagaatggag cgatgatatt ccccagcccc tgaccgacca gttcactatt cgccttgtgc
     3661 ttagtcccgg tcataccatc cagtaccaca gcgcagagca gagattcgat cgccccgagc
     3721 attgccattg agaatgccgc aggcagcagg gtgcgaatag aatcccaggt tagcgtgaat
     3781 tctgaattag gcagatccca cggcagcacc agttgcggca gcagttgcgg aataccgtta
     3841 ccctgagaac catcggccag gacgtagtgg aattgcgaac cgatggtagc aacatgtccg
     3901 ccgagcaggt taacaatccc catcaccgcg caaccagcca gcaaggccgg aaggtgaccg
     3961 ggtaaacgaa tgcccagacg cggccagaaa accagaatgc caagcgtcac aatgccaatg
     4021 gcagcatcac ccacattaat ggtcggcagc gccataaata atgcgccgac tttttgtaga
     4081 taatgttccg ggacatgggc catttgcaga ccgagaaaat ctttaatctg catggtaccg
     4141 atggtgatcc cgatacccga ggtgaaacct aaggtgacgg aaaccggaat atactcaatc
     4201 agacgaccaa agcgtgccag ccccatcaga atcaaaaaga ttcccgacaa caaggtcgca
     4261 accagcagtc ctgccagtcc aaactgttgc gaaacgggat agagaattac cacaaatgcc
     4321 gcagtcggac cggaaacgct aaagcgtgac ccacccgtta gagcaatgac aatccccgca
     4381 acagctgcgg tatataaacc gtactggggg gccacaccac taccaatagc caacgccatc
     4441 gccagcggga tagcaataat cccgacggtt atcccggcaa tcaggtcacg ggtaaaccgt
     4501 gcggcagtat atttttcttt ccagcaaacg tcgatcagag cgcggaaagg catcacatgt
     4561 gaggaaaata ttttgttcac aataatgttt catccgtgag cgcatcatct gtcaactaaa
     4621 tggcaggtga aggaggcata ggtcatacaa atggatatta cagacaaaaa aacccgccgc
     4681 agcgggtctt tgagccgggt tcgattagtg ttcgaacatg gcagagatcg attcttcgtt
     4741 gctgatacga cgaatcgctt cggccagcat acctgacagg gtcagagtac gcacgttcgg
     4801 cagtgatttg atttcatcgc tcagcggaat ggtatcgcag acaacgactt catcaattac
     4861 agagttacgc aggttgttcg ccgcgttgcc agagaagatc gggtgagtcg cgtacgcaaa
     4921 tacacgttta gcaccacgtt ctttcagcgc ttcagcagct ttacacagcg taccgccagt
     4981 gtcgatcata tcatcgacca gtacgcagtc acgacctgca acgtcaccga tgatatgcat
     5041 cacctgggaa acgttcgcac gcggacgacg tttgtcgatg attgccatat cggtatcgtt
     5101 cagcagctta gcgatagcgc gggcacgcac aacgccgccg atgtccggag aaaccacaat
     5161 tgggttatcc agattcagct gcagcatgtc ttccagcagg atcgggctac caaatacgtt
     5221 atcaaccgga acgtcgaaga aaccctgaat ctgttcagcg tgcagatcca ctgtcagcac
     5281 acggtcaaca ccgacgctgg agaggaagtc tgcaaccact ttcgcagtga ttggtacacg
     5341 agcggaacgg acgcgacggt cctggcgcgc atagccaaag taggggataa cagcggtgat
     5401 acgacctgcg gaagcacgac gcagggcatc aaccataacg actaattcca tcaggttgtc
     5461 gttagtaggg gcacaagtgg actggatgat gaaaatatca ccaccgcgta cattttcgtt
     5521 aatttgtacg ctgacttcgc cgtcgctaaa gcgacctaca gcggcgtcgc cgagtgaagt
     5581 gtacaggcgg ttggcaatac gttgtgctag taccggggtg gcgttaccag caaaaagctt
     5641 catatcaggc acgagaagaa cctcaggcat gcgtccattg gtggaaagaa tctgccgaaa
     5701 actgtgcggg ccaggcatga tcctttccag gcggtgtatt aaagagcgcg atgcaacgtc
     5761 tggaacaagg tgacgttgtc accgaaactc agcttgcccg gcttaaagca tggctctgtg
     5821 caatggggaa agattaacgc ctttcgccac aaagccattg agccattccg gggcttgctc
     5881 tagcacctgg cgggcttcag actctgtatc aaattcagca aagacacagg cccctgtccc
     5941 agtcaggcgt gacggggcgt attctaacag ccaggaaagc accgcatcaa cctcgcgaaa
     6001 acgttttctt gcgataacct cgcaatcatt gctgaattca cattttagca acgtttctat
     6061 tgaccttttt ggcgtattgc gcgggagttc aggatcttta aaaatcaccg gagtcggaat
     6121 acttacacca gggtgcgcca ccagatacca cttctctggc ggatccaccg gcgttagtat
     6181 ttcaccaacg ccttcggcaa acgcggcatg cccccgaaca aagacaggaa catctgcgcc
     6241 cagcgtcagc cccatttccg ccagctcatc catgcttagc ccgcattgcc agagatgatt
     6301 taatgccacc aggaccgtcg cggcattgga tgaaccaccg ccgagaccgc cgcccatcgg
     6361 caaacgcttg tcaatgctga tattcgcacc gcttcccgtc ggaagacgcc cgctgtctgc
     6421 cgcagttttc atcaacaacc gcgctgcgcg aacgatcagg ttatcttcat gttccacgcc
     6481 ttcaacgggc gttaacagac gaatatcccc atcgtcacga agctcaatgc tgatggtgtc
     6541 gccgtaatca agaaactgaa acagcgtttg cagcgtgtgg taaccatccg cacgctgacc
     6601 ggtaatgtat aaaaacagat taagttttgc cggagagggc cactgtgtcc gcattatttc
     6661 actatccagt tatccatttt taacttgatg cgttgaccac cgtcggtgag ttccatattg
     6721 gctggcatcg caggttgcgt tttggtgtca taaccaccat aaacaacctt ccagtttttg
     6781 ccattctggc tgtaggtaat ttcgctcagg cggtactggt cgtccagttt gtagtcggtt
     6841 gcatcacccg gtaaacctaa aatccactgg cgcaagctgt tgagcggaat tggcattccg
     6901 gtcaatttgc caatcatctc ttcggcgtca tcggcggtat aacgctgacc tttattgtcg
     6961 actaactgca cgttacccgg ttgagcattc agctccagtt ccgtgctgcc caatgggtta
     7021 gtgagcagca gacggtagcg atcctggccg gtttgctgcc agaaaaagcg ggcgtacact
     7081 ttttgttggt cagaaatata agcgaacgcg ccgcgagtct gatactgatt aagattgcgc
     7141 acgtcttgct gatgctgacg ccattgtggc gaatccgggc ttttgccagg acctttgggc
     7201 gtggtaacgg aacaggcagt gagcacaaga gcagccagcg gtagcaggcg gataagacga
     7261 aaatcgggca ggggcatagt gatgacaagt ccttgagata cgttgcagtt ataaccctta
     7321 atgctagcgt taccgtccgc tatcgtctat gttcaagttg tcttaattgc cagaatctaa
     7381 cggctttcgg caattactcc aaaagggggc gctctctttt attgatctta cgcatcctgt
     7441 atgatgcaag cagactaacc ctatcaacgt tggtattatt tcccgcagac atgacccttt
     7501 tagcactcgg tatcaaccat aaaacggcac ctgtatcgct gcgagaacgt gtatcgtttt
     7561 cgccggataa gctcgatcag gcgcttgaca gcctgcttgc gcagccgatg gtgcagggcg
     7621 gcgtggtgct gtcgacgtgc aaccgcacgg aactttatct tagcgttgaa gagcaggata
     7681 acctgcaaga ggcgttaatc cgctggcttt gcgattatca caatcttaat gaagaagatc
     7741 tgcgtaaaag cctctactgg catcaggata acgacgcggt tagccattta atgcgtgttg
     7801 ccagcggcct ggattcactg gttcttgggg agccgcagat cctcggtcag gttaaaaaag
     7861 cgtttgtcga ttcgcaaaaa ggtcatatga aggccagcga actggaacgc atgttccaga
     7921 aatctttctc tgtagcgaaa cgcgttcgca ctgaaacaga tatcggtgcc agtgctgtgt
     7981 ctgtcgcttt tgcggcttgt acgctggcgc ggcagatctt tgaatcgctc tctacggtca
     8041 cagtgttgct ggtaggcgcg ggcgaaacca tcgagctggt agcgcgtcat ctgcgcgaac
     8101 ataaagtaca gaagatgatt atcgccaacc gcactcgcga acgtgcccaa attctggcag
     8161 atgaagttgg cgcggaagtg attgccctga gtgagatcga cgaacgtctg cgcgaagccg
     8221 atatcatcat cagttccacc gccagcccgt taccgattat cgggaaaggc atggtggagc
     8281 gcgcattaaa aagtcgtcgt aaccaaccaa tgctgttggt ggatattgcc gttccgcgcg
     8341 atgttgagcc ggaagttggc aaactggcga atgcttatct ttatagtgtg gacgatctgc
     8401 aaagcatcat ttcgcacaac ctggcgcagc gtaaagccgc agcggttgag gcggaaacta
     8461 ttgtcgctca ggaaaccagc gaatttatgg cgtggctgcg agcacaaagc gccagcgaaa
     8521 ccattcgcga gtatcgcagc caggcagagc acgttcgcga tgagttaacc gccaaagcgt
     8581 tagcggccct tgagcagggc ggcgacgcgc aagccattat gcaggatctg gcatggaaac
     8641 tgactaaccg cttgatccat gcgccaacga aatcacttca acaggccgcc cgtgacgggg
     8701 ataacgaacg cctgaatatt ctgcgcgaca gcctcgggct ggagtagcag tacatcattt
     8761 tcttttttta cagggtgcat ttacgcctat gaagccttct atcgttgcca aactggaagc
     8821 cctgcatgaa cgccatgaag aagttcaggc gttgctgggt gacgcgcaaa ctatcgccga
     8881 ccaggaacgt tttcgcgcat tatcacgcga atatgcgcag ttaagtgatg tttcgcgctg
     8941 ttttaccgac tggcaacagg ttcaggaaga tatcgaaacc gcacagatga tgctcgacga
     9001 tcctgaaatg cgtgagatgg cgcaggatga actgcgcgaa gctaaagaaa aaagcgagca
     9061 actggaacag caattacagg ttctattact accaaaagat cctgatgacg aacgtaacgc
     9121 cttcctcgaa gtccgtgccg gaaccggcgg cgacgaagca gcgctgtttg caggcgatct
     9181 gttccgtatg tacagccgtt acgccgaagc ccgccgctgg cgggtagaaa tcatgagcgc
     9241 cagcgagggt gaacatggtg gttataaaga gatcatcgcc aaaattagcg gtgatggtgt
     9301 gtatggtcgt ctgaaatttg aatccggcgg tcatcgcgtg caacgtgttc ctgctacgga
     9361 atcgcagggt cgtattcata cttctgcttg taccgttgcg gtaatgccag aactgcctga
     9421 cgcagaactg ccggacatca acccagcaga tttacgcatt gatactttcc gctcgtcagg
     9481 ggcgggtggt cagcacgtta acaccaccga ttcggcaatt cgtattactc acttgccgac
     9541 cgggattgtt gttgaatgtc aggacgaacg ttcacaacat aaaaacaaag cgaaggcgct
     9601 ttcagtgctc ggtgctcgca tccacgctgc tgaaatggca aaacgccaac aggccgaagc
     9661 gtctacccgt cgtaacctgc tggggagtgg cgatcgcagc gaccgtaacc gtacttacaa
     9721 tttcccgcag gggcgcgtta ccgatcaccg catcaacctg acgctctacc gcctggatga
     9781 agtgatggaa ggtaagctgg atatgctgat tgaaccgatt atccaggaac atcaggccga
     9841 ccaactggcg gcgttgtccg agcaggaata atggaatatc aacactggtt acgtgaagca
     9901 ataagccaac ttcaggcgag cgaaagcccg cggcgtgatg ctgaaatcct gctggagcat
     9961 gttaccggca gagggcgtac ttttattctc gcctttggtg aaacgcagct gactgacgaa
    10021 caatgtcagc aacttgatgc gctactgaca cgtcgtcgcg atggtgaacc cattgctcat
    10081 ttaaccgggg tgcgagaatt ctggtcgttg ccgttatttg tttcgccagc gaccttaatt
    10141 ccgcgcccgg atacggagtg tctggtggag caggcactgg cgcggttgcc tgaacaacct
    10201 tgccgtattc tcgatctcgg gacgggtacc ggggcgattg cgcttgcgct ggctagcgag
    10261 cgcccggact gcgaaattat cgctgtagat cgtatgcctg atgctgtctc cctggcacaa
    10321 cgtaatgccc agcatctggc gatcaaaaat atccacattc tgcaaagcga ctggtttagc
    10381 gcgctagccg ggcagcagtt tgcgatgatt gtcagcaatc cgccgtatat tgacgagcag
    10441 gacccacatc ttcaacaagg cgatgtccgc tttgagccgc tcactgcgct ggttgcggca
    10501 gacagtggaa tggcagacat cgtgcatatc atcgaacagt cgcgtaacgc gctggtatcc
    10561 ggcggctttc tgcttctgga acatggctgg cagcagggcg aagcggtgcg acaagcattt
    10621 atcctcgcgg ggtatcatga cgtcgaaacc tgccgtgact atggtgataa cgagcgcgta
    10681 acgctcggcc gctattatca atgacaagtt tttctacact gcttagtgtt catcttatta
    10741 gtatcgcgct ttctgttggg ctattaacct tacgtttctg gctacgttat cagaagcatc
    10801 cacaggcatt tgctcgctgg acgcgcattg tgccgccggt tgtcgatacg gtgttattgt
    10861 taagcggcat tgcgttgatg gctaaagcgc acatcctgcc attttccggg caggcacagt
    10921 ggctgactga aaagctgttt ggagttatca tttatatcgt tttgggtttt attgcactcg
    10981 attatcgtcg tatgcacagt cagcaggcgc gcattattgc cttcccgctg gcgttggtgg
    11041 tgctgtacat catcattaaa ctcgccacca caaaagtacc gttactgggg taagtcatga
    11101 gatcgttagc tgatttcgaa tttaataaag cgccattgtg cgaaggcatg atcctggctt
    11161 gcgaagcaat ccgccgcgat tttccctcgc aagatgttta cgacgaactg gagcgtctcg
    11221 ttagtctggc gaaggaagaa atcagccagc ttctgccttt agaagagcag ctggaaaaac
    11281 tgatcgcgct gttttacggc gactggggat ttaaagcctc acgcggcgtt tatcgtcttt
    11341 ccgatgcatt atggctggac caggtgttaa agaatcgaca gggcagtgcg gtatcattag
    11401 gtgcggtttt attatgggtc gcgaatcgtc tcgatttgcc gctgctgccg gtgattttcc
    11461 ctacgcagct gatattgcgc attgaatgtc cggatggcga aatttggctg attaatcctt
    11521 ttaacggtga atcgttaagc gaacatatgc tggacggatg gttaaaggga aatatcagcc
    11581 cgtcggcgga actgttttat gaagaccttg atgaagctga taacattgag gtaatccgca
    11641 aattgctgga tacactcaaa gcctcgttga tggaagaaaa tcagatggag ctggcgttac
    11701 gcaccagcga agctttatta caattcaacc ctgaagatcc ctatgaaatt cgcgatcgtg
    11761 ggttgattta tgcgcaactg gattgcgaac acgttgcgtt gaacgattta agttatttcg
    11821 ttgaacggtg tccggaagac ccgatcagcg aaatgatccg tgcgcaaata aataacatcg
    11881 cgcataaaca tattgtgctg cattaattaa tcgacatttt actcaagatt aaggcgatcc
    11941 tatgaaacaa aaagtggtta gcattggcga catcaacgta gcaaatgacc tgccgttcgt
    12001 actgtttggc ggtatgaacg tgctggaatc tcgcgatctg gcgatgcgca tttgcgagca
    12061 ctacgtaact gtgacccaga aactgggtat cccttacgtg ttcaaagcct cttttgacaa
    12121 agccaaccgc tcctccatcc actcttatcg tggaccgggc ctggaagaag ggatgaaaat
    12181 cttccaggag ttgaagcaga cttttggcgt gaaaattatc accgacgttc acgaaccaag
    12241 tcaggcacag cccgttgctg atgtcgtgga tgtgattcag ttgccggcgt ttcttgctcg
    12301 ccagactgac ctggttgaag ccatggcgaa aaccggtgcg gtaattaacg tcaagaaacc
    12361 acagtttgtc agcccgggac agatgggtaa tatcgttgat aaattcaaag aaggcggcaa
    12421 cgaaaaagtg attctttgcg atcgtggtgc taacttcggc tatgacaacc tggttgtcga
    12481 tatgctgggc ttcagcatta tgaagaaagt gtctggtaac tcgccggtga ttttcgacgt
    12541 gacccacgca ctgcaatgcc gcgatccgtt tggcgcagct tccggtggtc gtcgtgctca
    12601 ggtggctgag ctggcacgag ccggtatggc ggtaggtctg gcggggctgt ttattgaagc
    12661 gcatccggat ccggaacatg cgaaatgtga tggtccatcc gcgctgccgc tggctaaact
    12721 ggaaccgttc ctcaagcaga tgaaagcgat tgatgatctg gtgaaaggtt tcgaagaact
    12781 ggataccagc aagtaatctt ttttgcttga aaaataaagt cttagcgttc tgcgttaaga
    12841 cttttttcat gggtgccaga tacaaaaaag gccgcaggct gttacccctg cggccggttt
    12901 cgggcgcata ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc
    12961 caccagctga caatcgctgc ggcaataatc cccgccagga tcggtgctgc caggtcgtgc
    13021 cagaaagtca tggcaaactg cgcgagcgtc atatagccgc cttgttgtaa tgacaacatt
    13081 ttgcggctat tcttgaagtg tctggtttca agattagccc ccgttttgtt gtcaggtttt
    13141 acctctcaac gtgcgggggt tttctctttc cagcaaccaa tgccaccagg gataaagccc
    13201 ccgcaacatt gcgcctcacc ggataattcc ggcttggtgt ggatactacg tctcaattca
    13261 tcttcacttc atccctgaaa tgtttgcaat aaagagtaca ttccggcttt tcaacagctg
    13321 ttgcagtggt ttcgtgcagg ttgtggtaca ggctcgcaat tctgattaac gagctgtcca
    13381 ggtagtatga agaaatacaa taagcaggaa tttatcttat ctctcccgat ttccgtcatc
    13441 ggaggagata taactgtcag gcaaatatcg tcatcaaata ggcggcaaac agtgccagat
    13501 gcgctgcgcc attgagcacg ttagtacgtc cggtggagaa ggagatatgg cacagcacta
    13561 aagaggccac catcaccacc atttctggcg caccaagtgc aaactgcaat tcgttacccg
    13621 tcataaaggc aattagcgtg acgacaggta cggtaagcga aattgttgct aacactgaac
    13681 caaagaacag attcatcgcg cgctgaacct ggttgttcaa cactgctttt aacgcaccta
    13741 aaccttccgg cgacagaatc aacagtgcca ccaggaagcc agtaaaggca acaggggcat
    13801 tcatgctgtc gagcaatgtc tccagcgagc tggcgttcat tttggtgact gcaataacgg
    13861 caatcagatg gataatcaac cagatagcat gccacaggct gctatgggca gacgttttac
    13921 cgtggtgcgg gtcgtcgtca tcactgtcat cttcgtgctc gtagacaaac aaactttgat
    13981 gcgttttggc ctggatcagc aaaaatacgc catacattgc cgcagaaatt aatgctacca
    14041 gtaacgcctg accggttgaa aaattcgccg caggcagagc cattggaaat accagtacga
    14101 ttatcgccag ggggaacagc gcaattaaat actgcttgat accaaacaga ttcatatatt
    14161 gggtggcaaa cttacgaccg cccaacaata atgaaaagcc aaccagccca ccggtaacaa
    14221 tcataatgat tgaatagagc gtatcacgca ttagcgttgg cgcggcgtcg ccggttgcca
    14281 ttaaagctga aatcaaactg acttcaagaa taaccactga aaggctaaga ataagcgaac
    14341 cgtaaggttc tcccaggcga tgggctaata cgtccgcatg acggacaaca ctaaaggcgc
    14401 tacttaaaat accaataagc gcaagaagat tgatggcaat gaccactggt agtgtctggc
    14461 tacttcccca caggaacagc actaccagca ccagaaccgg gaaaataagc gaagtctcct
    14521 tgtggcgggt ttttaccgcc tcttgagcat ttgacattat ggttatccct ttgcagatga
    14581 atttatcgaa aatgtaaaaa ataggtagga aaaataacag aaagtgtctg gatatcggta
    14641 acattttacg aatttttacc ccgctgtcga ttttttactc attggggcat aaaaataagt
    14701 attacgttta gacaatgttt gttttagcgt ttcttaagaa cagtctgaca tgaaaattct
    14761 tatgttttgg caagagtaga tattgttgac cacacttaat gttcaacttt gtaaaaggag
    14821 tcaacgatgc cgtataaaac gaaaagcgat ctgccggaaa gcgtaaagca cgttctaccg
    14881 tctcatgccc aggatatcta taaagaagcg ttcaacagcg catgggatca atataaagat
    14941 aaagaagatc ggcgtgatga cgccagtcgc gaagaaaccg cgcataaagt ggcctgggct
    15001 gctgtgaagc atgaatatgc caaaggggat gatgataaat ggcataaaaa atcgtaaaac
    15061 cggtagctta gttaaagcta ttcgtgcggt gttgccttgc aagtggtccg tggattgcat
    15121 attgttccgt tagtggtttc aaaatgagcg gtaaaaatgt ccggaagaca ccaaaaagtt
    15181 gtcgcaggga agtatgcagt ggcggaggtg taaggtgata acgcgtgatt tcttgatgaa
    15241 tgccgattgt aaaacggcat ttggtgccat tgaagaatca ctcttatggt cagcagaaca
    15301 acgggcggct tcgctggcgg cgacgctggc ttgtcgacct gatgagggac cggtgtggat
    15361 cttcggttat ggatcgttga tgtggaatcc ggcactggag tttaccgaat catgcaccgg
    15421 tacactggtt ggatggcatc gcgcgttttg cctgcgcctg accgccgggc gcgggactgc
    15481 gcaccagccg ggacggatgc ttgcactgaa agagggcgga cgcaccacag gcgtcgccta
    15541 tcgactacca gaagagacgc tggagcagga actaaccctg ttgtggaagc gcgagatgat
    15601 taccggctgt tatctgccaa cctggtgtca gcttgatctt gatgatggac gcacagtaaa
    15661 cgccattgtg tttattatgg acccgcgaca tccagaatat gaatctgaca ctcgcgctca
    15721 ggtcatcgcg ccgttgattg cggcggcgag cggtccgctg ggaaccaatg cacaatacct
    15781 gttttcactg gaacaggagc ttatcaaact gggaatgcag gacgatgggc tgaatgattt
    15841 gctggtatcg gtaaaaaaac tactggcgga gaattatccg gatggtgtgt taaggccggg
    15901 attcgcctga gtaaacttcc ctcatagtgg ggcgtcagac gcccccttaa acattaaaat
    15961 gtgagcactt tatcggctga cagcgtccat tgcgccagtt ccacaagagt accgatttcc
    16021 accccatcaa tcagaggaag tgtgctaatc ccgcgcccgt cggtacaggt tttgcacaat
    16081 ttcaccggta cattctgagc ggtaaggatc tccagcattt gctgaatgtt gtagccttcc
    16141 cctggttttt gcccgcgcaa cccggctgtg accgcatcag acatcaggaa cagacgcaga
    16201 tccagattgc tctcctgctc tcgtaacgca atggccagcc gcaagctgtt aaacaaggat
    16261 tcgctcccgt aaggtgcgcc attggcaacg atcactattt tttgcattat ttactcctgt
    16321 attcagggaa ttagacactc atcttctatc ttactgcttc tgcagcgtct gaccaatcgg
    16381 tcacattttt aaggattttc ctgaaagcgc gagaaaatac gacaaaagtt gccagtaatc
    16441 gttattcttt aaggctatgg tttttcatta ttaccggaag ttaccgacgt tttgagccgt
    16501 ttcgttcctc gcattatccc gttttattta ctcttgcttg tagcaggcgg tacagctaac
    16561 gcacaatcta ccttcgagca aaaagcggca aatccctttg ataataacaa tgatggtctg
    16621 ccggatttag gcatggctcc cgaaaatcat gatggggaaa aacactttgc tgaaattgtg
    16681 aaagatttcg gcgaaaccag tatgaatgat aacgggctgg atactggcga gcaggcaaaa
    16741 gctttcgcat tgggaaaagt ccgcgacgcg cttagtcaac aggttaatca gcacgtagag
    16801 tcctggctat caccgtgggg aaatgccagt gttgacgtca aagtggataa cgaaggacat
    16861 ttcaccggca gtcgtggaag ctggtttgtg ccgttacaag ataatgatcg ttatctcacc
    16921 tggagccagc ttggtcttac tcagcaggat aatgggttgg tgagcaatgt gggcgttggg
    16981 caacgctggg cgcgcggcaa ctggctggtg ggttataaca ctttttatga caacttgcag
    17041 gacgaaaatc ttcagcgagc gggctttggt gccgaagcgt ggggcgaata tttgcgatta
    17101 tcggcaaact tttatcagcc gtttgctgca tggcatgaac agacagccac gcaggaacaa
    17161 cggatggcgc gcgggtacga cctgacagct cggatgcgca tgccgttcta tcaacacctc
    17221 aataccagtg tcagcctaga acagtatttt ggtgatcgtg ttgatttgtt taactctggt
    17281 acgggttatc acaatcccgt cgcgttgagt ctgggattaa attacacccc tgtgccatta
    17341 gtcactgtga cggcccagca taaacagggg gaaagtggcg aaaatcaaaa taacctcggg
    17401 ctgaatctta attaccgctt tggtgtaccg ctcaaaaaac aactttctgc gggcgaggtt
    17461 gccgaaagtc agtcgttacg tggtagtcgc tatgacaatc cgcagcgaaa taatctaccg
    17521 actcttgagt accgacagcg aaaaacgtta acggtgtttc tggcgacacc gccgtgggat
    17581 ctaaaacctg gcgaaacagt gccgctgaaa ttacaaatcc gcagtcgtta cggtattcgg
    17641 caactgattt ggcagggcga tacgcagata ttaagtttga cgccgggcgc acaagccaac
    17701 agcgcggagg gctggacgct gatcatgcct gactggcaga acggggaagg ggcaagcaat
    17761 cactggcgat tgtctgtggt ggtggaagat aaccaggggc agcgtgtctc ctccaatgag
    17821 atcacgctaa cgcttgtcga accgttcgat gcattgtcaa acgacgaact gcgctgggaa
    17881 ccgtaatcag aaaatgcgct cctgatgcac ccataccgct gcttccacgc gagacttgag
    17941 cttcattttc ttcagcatgt gcttgacgtg cacttttact gtgctttcgg tgatatccag
    18001 gcggcgggca atcatcttgt tcggcaaacc ctgggcaatc agcttgagaa tatcgcgctc
    18061 gcgtggggtt aactggttaa catcgcgctc agtagtggca cggttagcgc gcaagctggc
    18121 ggccagaaca ggcgttaatg cttcgcttaa taccatttcg ccagcagctg cctgatgcaa
    18181 tgctttcagc agatcttccg gttccatatc ttttaacaga tagccatccg cgccgcgttt
    18241 cagtgcggtg accacatctt cttcatggtt agagacgctg aataccacaa tgcgccctga
    18301 gagggacttt tcgcgcagtt tatccagcgt ttccagaccg ttcatgccgg gcatattgag
    18361 atctaacagg atcagatcgg gatcaagaga ttccgccagt tcaataccct gttcgccatt
    18421 actcgcttcg ccaaccacgg tgatatctgg tgccatactg ataagctgtt ttacgccagt
    18481 tcgcagcatc gggtgatcgt caatcagcag gatagtagcc ggttcctgat tactcatggg
    18541 tatctccttg gacgtctgtg aaagtttttt cgggaataaa ggtgaccacc acttcggtgc
    18601 cacctgattc acgacggcgg acgcggcaat cgcctcgtaa actttgcgca cggtcgcgca
    18661 ttattatcat gccgtagtga ttgctgcgga tggcattttc aggcacgcca cagccgttat
    18721 cctggacggt cagtttgacc tgattatcgt tttgcgccac cgtcaccacg acctcactcg
    18781 cttgcgaatg tttgagggcg ttacttaatg cctcacgggc aatttgcaac aagtggattg
    18841 cctgatgtga aggcaccaga cgaggcggca attgataatc cagcttcacc gggaagccaa
    18901 atttggcgct gtactcttcg caactcgctt ccagcgccgg acgtaatcca ggctcggtga
    18961 gctgcaagcg gaatgtggtg agcaattcac gcaactgcgc ccaggatgca ttcagttcgt
    19021 tacggatctg acttaacagt tcgcggctgc tttctggcag cgcatcgccc tgcatctgta
    19081 aacaactcac ctgcatcttc atgcaagaga gagattgggc aatagaatca tgcagttcgc
    19141 gcgcaatggt ggcacgctct tccatcacga tcaactgttg ctgacgttcc tgatggcgat
    19201 ccagcgccag cgtggcggta agttgttcaa ccagggtatc caccagttgt tgttggtcat
    19261 ggctaagatg acgcccctgc ggcagggtcg ccagcaaaat accgtactgc gtatgagagt
    19321 cagccagccg ccacttcagg gtcgtgccgc gatcaccaac gggtaatacg ccgcgcgggc
    19381 agagctggca gcctttatca tcacaagtca tatctggctg gcaggtaaac tcctgatgat
    19441 tctcttcatc atcagtgtca tacacccgca gttcgatatc acgtagcagg gttaaattct
    19501 gtaagccgtt gagtaccggt gacaggcgtt cacacagcgg ggcgcgggaa tgcaaacggc
    19561 ggttagcctg ccataaaaaa gagaggatct gatttttatg ctccagcccg gcggttttct
    19621 cctgaacccg ctgctcaagt acggcataac tttcggccag ttctgcagac atattgttca
    19681 acgcagttcc aagcatcgcc atttcgttgc gcccgctgat gtttgcgcgt tgggtaaaat
    19741 cgcgatggct gacggcactc gccattgcca gcagttgccg ccacggttgt agcagtcgcg
    19801 cccgcaacca gataatagtg aacaccagta aaagtgccat aaataccgcc attacccgat
    19861 ggaccagtac cactgtctca atacgcattt ccgtggtgcg gtcaaaacca gataccagct
    19921 gatcaagccc ggcaacaaac tggctgacat ccgctgacac cgtttctcgg ttttgtgcac
    19981 gcatcagcgc agggatcagt tcattacgcc agtaatcttg taagccctgt aattgcgcca
    20041 gttgtccgtc tcgttctgct gctcgagtca actcggcgct aaatgccgtt tgttccatct
    20101 ctttaattaa gggcttgtct ttctcgctta atggcactgc cgccaacaga cggtaacttt
    20161 gcatgcgcag cgatcccgct ttgttgatcg catgggcgct gccctgaacg ccttgcacca
    20221 gccagccaga aaccgccatc cctgccagtc caatagcagt agaaagcaac acaataagcg
    20281 caacctgatt aaccagggtg agcggagaga gacaacgttt aagcatgtaa accccttcct
    20341 tcaggcttta aatgagcaat aaccttaatg aatgtgacga tatattctgg aatggcagta
    20401 ttctcggcta ttggctgaag tatacccata gccggaaaga gttactcctt atttgccgtg
    20461 tggttagtcg ctttacatcg gtaagggtag ggattctaca gcaccgtgaa aaatctcata
    20521 atttttatga aatcactgta ctcactatgg gtaatgataa atatcaatga tagataaagt
    20581 tatcttatcg tttgatttac atcaaattgc ctttagctcc agacactaag gtggcagaca
    20641 tcgaaacgag tatcagaggt gtctatgagt cactcatctg cctccgaaag ggctactgga
    20701 gctgtcataa cagattggcg accggaagat cctgcgttct ggcaacaacg cggtcaacgt
    20761 attgccagcc gcaacctgtg gatttccgtt ccctgtctgc tgctggcgtt ttgcgtatgg
    20821 atgttgttca gcgctgttgc ggtgaactta ccgaaagtcg gttttaattt tacgaccgat
    20881 cagctattta tgttgactgc gctgccttcg gtttctggcg cgttattacg tgttccatac
    20941 tcctttatgg ttcctatctt cggtggtcgt cgctggacgg cgttcagcac cggtattctg
    21001 attattcctt gcgtctggct gggttttgcc gtgcaggata cctccacgcc ttatagcgtc
    21061 ttcatcatca tctctctgct atgcggcttt gctggcgcga acttcgcatc cagtatggca
    21121 aacatcagct tcttctttcc gaaacagaag cagggtggcg cgctgggtct gaatggtggt
    21181 ctgggaaaca tgggcgtcag cgtcatgcag ttggttgctc cgctggtggt atcactgtcg
    21241 attttcgcag tatttggtag ccagggcgtc aaacagccgg atgggactga gctgtatctg
    21301 gcgaatgcgt cctggatatg ggtgccgttc cttgccatct tcaccattgc ggcgtggttt
    21361 ggcatgaacg atcttgctac ctcgaaagcc tccatcaagg agcagttgcc ggtactcaaa
    21421 cggggtcatc tgtggattat gagcctgctg tatctggcaa ccttcggctc cttcatcggc
    21481 ttctccgcgg gctttgcgat gctgtcaaaa acgcagttcc cggatgttca gattctgcaa
    21541 tacgctttct tcgggccgtt tattggtgcg ctggcgcgtt ctgcaggtgg tgcattatct
    21601 gaccgtctgg gcggaactcg tgtcacgctg gtgaacttta ttctgatggc gattttcagc
    21661 ggcctgctgt tcctgacctt accgactgac gggcagggcg gaagcttcat ggcgttcttc
    21721 gcggtcttcc tggcgctgtt cctgacagct gggctgggta gtggttccac tttccagatg
    21781 atttcagtga tcttccgtaa actgacaatg gatcgcgtga aagcagaagg gggttctgac
    21841 gaacgtgcga tgcgtgaagc ggcaaccggc acggcagcgg cgctgggttt catctctgcg
    21901 attggcgcga ttggtggctt ctttatcccg aaagcgtttg gtagctcgct ggcattaacg
    21961 ggttcgccag taggtgcaat gaaagtattt ttgattttct atatcgcctg cgtagtgatt
    22021 acttgggcgg tatatggtcg gcattctaaa aaataaatct taatatttga ttatcctttg
    22081 cgcggcatga tgccgcgctt tttttatgcg tcatttagtt acaacatatt agtgttatat
    22141 ggcttattt
//
LOCUS       DAZPDE010000052        21913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPDE010000052 DAZPDE010000000
VERSION     DAZPDE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.052"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHU0J_001832"
     CDS             complement(<1..130)
                     /locus_tag="ACHU0J_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1216554.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVIN"
     gene            complement(140..691)
                     /gene="ybcL"
                     /locus_tag="ACHU0J_001833"
     CDS             complement(140..691)
                     /gene="ybcL"
                     /locus_tag="ACHU0J_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1216555.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(860..1192)
                     /gene="emrE"
                     /locus_tag="ACHU0J_001834"
     CDS             complement(860..1192)
                     /gene="emrE"
                     /locus_tag="ACHU0J_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1216556.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1537..1851)
                     /gene="fliE"
                     /locus_tag="ACHU0J_001835"
     CDS             complement(1537..1851)
                     /gene="fliE"
                     /locus_tag="ACHU0J_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1216557.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2065..3724
                     /gene="fliF"
                     /locus_tag="ACHU0J_001836"
                     /pseudo
     CDS             2065..3724
                     /gene="fliF"
                     /locus_tag="ACHU0J_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3717..4712
                     /gene="fliG"
                     /locus_tag="ACHU0J_001837"
     CDS             3717..4712
                     /gene="fliG"
                     /locus_tag="ACHU0J_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1216558.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4705..5391
                     /gene="fliH"
                     /locus_tag="ACHU0J_001838"
     CDS             4705..5391
                     /gene="fliH"
                     /locus_tag="ACHU0J_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1216559.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5391..6764
                     /gene="fliI"
                     /locus_tag="ACHU0J_001839"
     CDS             5391..6764
                     /gene="fliI"
                     /locus_tag="ACHU0J_001839"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1216560.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6786..7127
                     /gene="fliJ"
                     /locus_tag="ACHU0J_001840"
                     /pseudo
     CDS             6786..7127
                     /gene="fliJ"
                     /locus_tag="ACHU0J_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7124..8251
                     /locus_tag="ACHU0J_001841"
     CDS             7124..8251
                     /locus_tag="ACHU0J_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1216561.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8356..8820
                     /gene="fliL"
                     /locus_tag="ACHU0J_001842"
     CDS             8356..8820
                     /gene="fliL"
                     /locus_tag="ACHU0J_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1216562.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8825..9829
                     /gene="fliM"
                     /locus_tag="ACHU0J_001843"
     CDS             8825..9829
                     /gene="fliM"
                     /locus_tag="ACHU0J_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1216563.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9826..10239
                     /gene="fliN"
                     /locus_tag="ACHU0J_001844"
     CDS             9826..10239
                     /gene="fliN"
                     /locus_tag="ACHU0J_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1216564.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10242..10607
                     /gene="fliO"
                     /locus_tag="ACHU0J_001845"
     CDS             10242..10607
                     /gene="fliO"
                     /locus_tag="ACHU0J_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1216565.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10607..11372
                     /gene="fliP"
                     /locus_tag="ACHU0J_001846"
                     /pseudo
     CDS             10607..11372
                     /gene="fliP"
                     /locus_tag="ACHU0J_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11382..11651
                     /gene="fliQ"
                     /locus_tag="ACHU0J_001847"
     CDS             11382..11651
                     /gene="fliQ"
                     /locus_tag="ACHU0J_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1216566.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11660..12445
                     /gene="fliR"
                     /locus_tag="ACHU0J_001848"
     CDS             11660..12445
                     /gene="fliR"
                     /locus_tag="ACHU0J_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1216567.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12730..13354
                     /gene="rcsA"
                     /locus_tag="ACHU0J_001849"
                     /pseudo
     CDS             12730..13354
                     /gene="rcsA"
                     /locus_tag="ACHU0J_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13398..13586)
                     /gene="dsrB"
                     /locus_tag="ACHU0J_001850"
     CDS             complement(13398..13586)
                     /gene="dsrB"
                     /locus_tag="ACHU0J_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1216568.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13749..13927
                     /locus_tag="ACHU0J_001851"
                     /pseudo
     CDS             13749..13927
                     /locus_tag="ACHU0J_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2525 domain-containing protein"
     gene            14225..15040
                     /locus_tag="ACHU0J_001852"
                     /pseudo
     CDS             14225..15040
                     /locus_tag="ACHU0J_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15037..16731)
                     /gene="dgcQ"
                     /locus_tag="ACHU0J_001853"
                     /pseudo
     CDS             complement(15037..16731)
                     /gene="dgcQ"
                     /locus_tag="ACHU0J_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(16902..17084)
                     /locus_tag="ACHU0J_001854"
     CDS             complement(16902..17084)
                     /locus_tag="ACHU0J_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1216569.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(17163..18080)
                     /locus_tag="ACHU0J_001855"
     CDS             complement(17163..18080)
                     /locus_tag="ACHU0J_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1216570.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18253..19173
                     /gene="yedA"
                     /locus_tag="ACHU0J_001856"
     CDS             18253..19173
                     /gene="yedA"
                     /locus_tag="ACHU0J_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR1216571.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19162..19632)
                     /gene="vsr"
                     /locus_tag="ACHU0J_001857"
     CDS             complement(19162..19632)
                     /gene="vsr"
                     /locus_tag="ACHU0J_001857"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1216572.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19613..21031)
                     /gene="dcm"
                     /locus_tag="ACHU0J_001858"
     CDS             complement(19613..21031)
                     /gene="dcm"
                     /locus_tag="ACHU0J_001858"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1216573.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21098..21599)
                     /locus_tag="ACHU0J_001859"
                     /pseudo
     CDS             complement(21098..21599)
                     /locus_tag="ACHU0J_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(21678..>21913)
                     /gene="drpB"
                     /locus_tag="ACHU0J_001860"
     CDS             complement(21678..>21913)
                     /gene="drpB"
                     /locus_tag="ACHU0J_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1216574.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         5367 a         5781 c         5571 g         5194 t
ORIGIN      
        1 aattgataac tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc
       61 tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctc
      121 ttgccagcat actcttaccc tattttatct cataaactgg tgttatctca gcggttgcaa
      181 ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa
      241 tcttatcagt ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg
      301 gaggacatgc tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag
      361 tcggaagttt tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg
      421 gaatattagc aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca
      481 cagttacggc aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg
      541 tattaccacc ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg
      601 tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg
      661 ccaatacact tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta
      721 ttttatttga tgtgccttga tctcattatg cataaaggcg caatgatcgc gcaaatgcaa
      781 tcataattat aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta
      841 tctcaaggag aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca
      901 caccggcaca aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa
      961 atccccagga aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa
     1021 tccctgtagg tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa
     1081 taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg
     1141 taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt
     1201 ctttcctgtt catactggag agaattgtac tacagtttga acacaactca ccgttttcat
     1261 cctggtgtat gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa
     1321 tgatcagaag gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga
     1381 caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc
     1441 cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga
     1501 tataattaaa caagcatagt caacaggtta cacaacctac acctgcatgg acatcacttc
     1561 ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc
     1621 tttttgcata tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt
     1681 ttctgcctgc gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag
     1741 ctgcccggca aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc
     1801 cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc
     1861 ccggataatt tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag
     1921 caacaaaaaa actggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt
     1981 caatttttcg agtgtgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt
     2041 ccataatcag ccacgaagtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag
     2101 tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca
     2161 gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc
     2221 agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt
     2281 ccttaccgct tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa
     2341 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat
     2401 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
     2461 ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg
     2521 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
     2581 gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg
     2641 gtttccagcg cggttgctgg tctgccgcct ggaaacgtca cgctggtgga tcagggcggg
     2701 catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat
     2761 gccagcgatg tcgaaggccg tattcagcag cgtattgaag cgatcctgtc acctattgtt
     2821 ggtaacggta atattcacgc ctaggtcacg gcgcaactgg acttcgccag taaagaacaa
     2881 acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag
     2941 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
     3001 aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac
     3061 cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa
     3121 accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg
     3181 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
     3241 cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt
     3301 tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa
     3361 agcggcggag cactgccgtt ttggcaacag caagtgttta tcgatcagtt gctggctgcc
     3421 ggtcgctggt tgctggttgc tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt
     3481 acgcccgcag ctaacacgtc gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc
     3541 ccgcgaggaa gtggaagatg cggtggaagt ccgtctgagc aaagacgaac aactccaaca
     3601 acggcgcgct aaccaacgac tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc
     3661 tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga
     3721 gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg
     3781 cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg
     3841 cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag
     3901 aagctgaaca gtttgctgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg
     3961 tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga agatattctc gaaacccgcg
     4021 ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga
     4081 ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgagg cgcgcccagg
     4141 ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg
     4201 ccacctttgg cggcgtgcag ccagccgcgc tggccgaact gaccgaagta ctgaatggct
     4261 tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa
     4321 ttatcaacct gatgaaaacg cagcaggaag aagccgttat taccgccgtg cgtgaattcg
     4381 acggcgagct ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg
     4441 tcgatgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg
     4501 cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg
     4561 ccgccgatat tctgcgcgac gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg
     4621 aaaacgaaca gaaagcgatt ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg
     4681 tgattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg
     4741 gacgatctcg cgccaccacc ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt
     4801 attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccac
     4861 gagcagggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcacga acagggctat
     4921 caggaaggac tggcccaggg gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg
     4981 ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt
     5041 gatagtgtga tagcttcgcg cctgatgcag atggcgctgg aggcagcacg tcaggtgatc
     5101 ggtcagacac caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag
     5161 caagaaccgt tattcagcgg gaaaccacag ctgcgcgtac acccggatga tctgcaacgt
     5221 gtggatgata tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg gggcgatccc
     5281 accctccacc ctggcggatg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc
     5341 gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc
     5401 gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg
     5461 cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat
     5521 tacaattgcc gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg
     5581 aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg
     5641 aagtggaagg tgtcctgccc ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc
     5701 tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta
     5761 gcggtaaacc gctcgatggc ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta
     5821 ccccgccatt taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc
     5881 gcccaatcaa tgccctgctt accgtcggac gcgggcagcg tatggggctg tttgccgggt
     5941 cgggcgttgg taaaagtgtg ctgctgggga tgatggcacg ctacacccgc gccgatgtca
     6001 ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa agattttatt gagaatatcc
     6061 tcggtgccga aggacgtgca cgctcagtgg tgattgccgc tccggcggat gtttctccgc
     6121 tcctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg
     6181 gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa
     6241 ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca
     6301 aattaccggc actggtcgag cgtgccggta atggcattag cggcggcggc tcgattaccg
     6361 cgttttatac cgtgctcacc gaaggcgatg accagcagga ccccattgct gactccgcgc
     6421 gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc
     6481 cggctatcga tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac
     6541 attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc
     6601 tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc
     6661 tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag
     6721 cgtctctcca ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc
     6781 agatggcgga agaacagctc aaaatgttga tcgattatca gaatgaatat cgcaataacc
     6841 tcaatagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta
     6901 tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga
     6961 aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga
     7021 cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa
     7081 agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc
     7141 cttgattacc gccgacgttg acaccaccac attgcctggc ggcaaagcca gcgatgctgc
     7201 acaagatttt ctcacgttgt tgagcgaagc attagcaggc gagacaacaa ctgacaaagc
     7261 ggccccccag ttgctggtag caacagataa gcccacgaca aaaggcgagc cgctggtcag
     7321 cgatattctt gccgatgcgc aacaagcgga tttactgatc cctgtggatg aaacacttcc
     7381 tgtcatcaac gacgaacaat ccacatcaac accgttaacc accgctcaga cgatgacgtt
     7441 ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga
     7501 cgtcaccgca agtctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa
     7561 agtgactgat gcaccgtcaa ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact
     7621 gacttctgcg caactcacta cagcacagcc tgatgacgcc cccggcacac cagctcagcc
     7681 attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca cgccttcgcc
     7741 ggtgaccgct gacgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt
     7801 cgccgcgcct gttttgagtg caccgctggg ttctcacgaa tggcaacaat cattgagcca
     7861 gcatatttcg ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcacccaca
     7921 ggatttaggt gaagtgcaaa tttccctcaa agtggatgat aaccaggcgc agatccagat
     7981 gatttcaccg catcagcacg tacgcgccgc cctggaagca gcgctgccgg tactgcgtac
     8041 gcagctggcc gaaagtggca ttcagttagg gcaaagcaac attagtggcg aaagctttag
     8101 tggtcagcag caggccgctt cccagcaaca gcaaagccaa cgcacagcaa accatgaacc
     8161 tctggcgggg gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac
     8221 aggtaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct
     8281 tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca
     8341 ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg
     8401 atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg
     8461 cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc
     8521 ttctatgcgc tggatacctt tacggtcaat ttgggcgatg cggatcgcgt gctttatatc
     8581 ggcataaccc tgcgactgaa agatgaagca acccgctcgc ggctgagtga gtatttgccg
     8641 gaagtccgta gtcgcttgct gttactgttt tcacgtcagg atgctgccgt actggcgaca
     8701 gaagaaggca agaaaaacct gattgccgag attaaaacca cactttccac cccgcttgtt
     8761 gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa
     8821 cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag
     8881 cgaagtcaaa gacgaaccga cagccagtat tagcggcgaa agtgacattc gtccgtacga
     8941 tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga
     9001 gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgccgta gcccggacat
     9061 taccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc
     9121 gaccaacctg aacctaatcc acctgaaacc gctacgcggc acagggctgg tagtgttctc
     9181 gccgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac
     9241 caaagtggaa ggtcgcgagt ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa
     9301 gctggcgctg gaaggctata gcgacgcctg gaaagcgatt aatccgctgg aagttgagta
     9361 cgtgcgttcg gaaatgcagg tggaatttac caatatcacc acctcgccga acgacattgt
     9421 ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct
     9481 gccattcagc atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc
     9541 gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct
     9601 ggaactggtg gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa
     9661 acccggcgac gtcctgccga tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt
     9721 cccggttctg accagtcagt acggtactct caacggtcag tatgcgttac ggatagaaca
     9781 tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat
     9841 aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc gttgagcgag
     9901 caaaaatcaa ccagcgaaaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt
     9961 gatgtcagcg gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc
    10021 gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaagggtca
    10081 gtcgtggcgc tggacggtct ggcgggcgaa ccgctggata ttctgatcaa cggttattta
    10141 atcgcccagg gcgaagtggt ggttgttgcc gataaatatg gcgtgcggat caccgatatc
    10201 attaccccgt ccgagcggat gcgccgcctg agccgttagt gatgaataac catgctactg
    10261 tgcaatcttc cgcgccggtt tctgctgagc cactgctgca ggtgagcggc gcactcatcg
    10321 ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggctggga tttgccccta
    10381 aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac
    10441 gggtcgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa
    10501 tcaatctgct gcataaactt cccccttcag caccaacgga agagataccg cagaccgatt
    10561 ttcagtcggt catgaaaaat ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt
    10621 ttctgtcgca ccggtccttc tctggctgat tacgcccctc gccttcgcgc aactgccggg
    10681 tatcaccagc cagccgctac ctggcgggta tcaccagcca gccgctacct ggcggtggac
    10741 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    10801 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    10861 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    10921 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    10981 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11041 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11101 ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga
    11161 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11221 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11281 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11341 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11401 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    11461 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    11521 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    11581 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    11641 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    11701 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    11761 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    11821 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    11881 cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    11941 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt
    12001 cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata tgctggcatt
    12061 actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt
    12121 tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa
    12181 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgtt
    12241 gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt
    12301 tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc
    12361 gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat
    12421 tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc taaggattat
    12481 ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat atttgtttta
    12541 agctcactca catatcgcaa catttacttt aagacaattc caggcaaatt atacaacact
    12601 ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt
    12661 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
    12721 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
    12781 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
    12841 gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt
    12901 ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat
    12961 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
    13021 agaaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct
    13081 tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc
    13141 attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca
    13201 aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa
    13261 acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac tgacggataa
    13321 tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc
    13381 agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg atgccctgct
    13441 tcattaaaga accagatgcc gagcgggtag tcttccagcg caaccaggta cattgtgcct
    13501 tcactaaact cctcaactgc cagtaccact ccaggacgac gcggaccgcc atccgttttg
    13561 actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa
    13621 caatttcgtt tttctggcag cgccaagcgc gcgcgagtgc tgattttctc gacggtctat
    13681 acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa cggttgcgag
    13741 gaaagatgat gaaaaccgca aaagtgtaca gcgataccgc aaaacgtgag gtcagcgtcg
    13801 atgtcgccgc cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg
    13861 gcgtgaattg gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa
    13921 tcgttgaatg cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa
    13981 gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct
    14041 gcaatttttt tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa
    14101 tagaaatgtg acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa
    14161 aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc
    14221 gaccatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct
    14281 ggacagtcat agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc
    14341 caatattccc gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa
    14401 aatgttgggg ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc
    14461 tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga
    14521 aatctgccag gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga
    14581 tgatgtcgac gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct
    14641 gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc
    14701 acaatttatc gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg
    14761 gcacgtactg gatgcctctg ccggaaaaga tcaggctgcc aactggatta tcgcgaccta
    14821 tcaacaattg tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc
    14881 gcctttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt
    14941 acatctgcat gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg
    15001 ctggcgtgaa gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac
    15061 ccgattgcgc ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg
    15121 ttcaaaatca taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg
    15181 tatcgttgta ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc
    15241 tgcgacctcc gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc
    15301 accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg
    15361 agaaagaaca cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc
    15421 aagatcgacc tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg
    15481 acgggctttt tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc
    15541 ctgccactgc aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata
    15601 ccaggagagg agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat
    15661 actgccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc
    15721 gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt
    15781 gtcatgttcc attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt
    15841 tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc
    15901 accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat
    15961 actcatcccc agcacgccat accagtagtt attactgtct accggaacac tgacggtaac
    16021 ctgcggttca gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg
    16081 acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg
    16141 cgttggtaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga
    16201 gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc
    16261 aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt
    16321 cccctcgctg actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag
    16381 ttcgatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag
    16441 agaggtgaaa tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag
    16501 tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt
    16561 acgctagctg gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt
    16621 ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg
    16681 tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac
    16741 tcccaacctg cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg
    16801 ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca
    16861 ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa
    16921 cgactcccct cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa
    16981 ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg
    17041 ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt
    17101 cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag
    17161 tattaatgtg cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg
    17221 ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca
    17281 ctttgctgcc cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca
    17341 atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc
    17401 caaggcgcga taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc
    17461 gccagccaat accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc
    17521 acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct
    17581 gccacaatcc ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc
    17641 gcccctttaa ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga
    17701 cgctgctggc tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact
    17761 ttctctaccc cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc
    17821 cacgggataa acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc
    17881 gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact
    17941 tgttgcgcat tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt
    18001 ttgcccatca cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa
    18061 ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc
    18121 aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa
    18181 ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg
    18241 ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg
    18301 tatatcattt ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg
    18361 ttaatgatgg cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta
    18421 ctgcgcggac acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg
    18481 ttattgctgg ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc
    18541 ggcatcgccg ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg
    18601 tttggcatta aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc
    18661 atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta
    18721 atcggctcga ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta
    18781 gggatgatgg cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg
    18841 atgattgcgg gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc
    18901 tatctggcgc tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat
    18961 gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg
    19021 ggtacgggac tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt
    19081 gttttcgcgg tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc
    19141 gcgccagtta ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc
    19201 gctggcacct tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag
    19261 cttctcgcgc ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg
    19321 caagcgactg atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa
    19381 ctcagttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc
    19441 gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact
    19501 ggcgtcctga acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc
    19561 aatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg
    19621 aacgtcgacc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct
    19681 caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat
    19741 aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca
    19801 ttaagcgcgc gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat
    19861 cgtcaaagtc tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc
    19921 catctttgta ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa
    19981 ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt
    20041 acttccacag caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg
    20101 ccagcgtcac tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt
    20161 tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga
    20221 aatgtttacc atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt
    20281 catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac
    20341 tcttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc
    20401 gcaccacatc aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga
    20461 gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta
    20521 aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc
    20581 cttcttgatg actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt
    20641 cgcaataatg gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca
    20701 cgcactgtcc accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat
    20761 cgataaagcg aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca
    20821 gatgggcaaa ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt
    20881 ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt
    20941 taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt
    21001 atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg agatggccga
    21061 caggttaaca taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat
    21121 ctatcacctt gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca
    21181 tcaaacagcg ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc
    21241 cccagggctt ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg
    21301 atttgcgcgc tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc
    21361 ggaaattgca cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa
    21421 gaacgctgcc gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg
    21481 gttaaaatca ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag
    21541 acacggcgaa aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac
    21601 cagttctcga actatgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt
    21661 ctcaacctta gcagttttta ttcatagcgt ctgcaacgtg cggaacgagg tcgggtgtac
    21721 catgcaatgc cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct
    21781 cctaaccact tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg
    21841 gggatccggc tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat
    21901 gtatagaaag ccc
//
LOCUS       DAZPDE010000053        21508 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPDE010000053 DAZPDE010000000
VERSION     DAZPDE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.053"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHU0J_001861"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACHU0J_001861"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1216575.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHU0J_001862"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACHU0J_001862"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1216576.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACHU0J_001863"
     CDS             complement(3121..3420)
                     /locus_tag="ACHU0J_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1216577.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACHU0J_001864"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACHU0J_001864"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1216578.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHU0J_001865"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACHU0J_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR1216579.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHU0J_001866"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHU0J_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1216580.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHU0J_001867"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHU0J_001867"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1216581.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHU0J_001868"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHU0J_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACHU0J_001869"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACHU0J_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1216582.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACHU0J_001870"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACHU0J_001870"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR1216583.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACHU0J_001871"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACHU0J_001871"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR1216584.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACHU0J_001872"
                     /pseudo
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACHU0J_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13973..14797)
                     /locus_tag="ACHU0J_001873"
     CDS             complement(13973..14797)
                     /locus_tag="ACHU0J_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR1216585.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACHU0J_001874"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACHU0J_001874"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1216586.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACHU0J_001875"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACHU0J_001875"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR1216587.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="ACHU0J_001876"
     CDS             complement(18729..19202)
                     /locus_tag="ACHU0J_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1216588.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHU0J_001877"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHU0J_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR1216589.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHU0J_001878"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACHU0J_001878"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR1216590.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21508
                     /locus_tag="ACHU0J_001879"
     CDS             21362..>21508
                     /locus_tag="ACHU0J_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR1216591.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQI"
BASE COUNT         5405 a         5489 c         5277 g         5337 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgctgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaaatt
//
LOCUS       DAZPDE010000054        21255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPDE010000054 DAZPDE010000000
VERSION     DAZPDE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.054"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..793
                     /locus_tag="ACHU0J_001880"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHU0J_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHU0J_001881"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHU0J_001881"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1216592.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHU0J_001882"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHU0J_001882"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1216593.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHU0J_001883"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHU0J_001883"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1216594.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHU0J_001884"
     CDS             complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHU0J_001884"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1216595.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHU0J_001885"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHU0J_001885"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1216596.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="ACHU0J_001886"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="ACHU0J_001886"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1216597.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHU0J_001887"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHU0J_001887"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1216598.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="ACHU0J_001888"
     CDS             complement(12980..14320)
                     /locus_tag="ACHU0J_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1216599.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHU0J_001889"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHU0J_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1216600.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="ACHU0J_001890"
     CDS             complement(16108..16557)
                     /locus_tag="ACHU0J_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1216601.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHU0J_001891"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHU0J_001891"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1216602.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="ACHU0J_001892"
     CDS             complement(17357..17686)
                     /locus_tag="ACHU0J_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1216603.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACHU0J_001893"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACHU0J_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1216604.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="ACHU0J_001894"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="ACHU0J_001894"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1216605.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="ACHU0J_001895"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="ACHU0J_001895"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1216606.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5671 c         5068 g         5288 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccatcata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAZPDE010000055        21254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPDE010000055 DAZPDE010000000
VERSION     DAZPDE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.055"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            138..749
                     /gene="ruvA"
                     /locus_tag="ACHU0J_001896"
     CDS             138..749
                     /gene="ruvA"
                     /locus_tag="ACHU0J_001896"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1216607.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            758..1768
                     /gene="ruvB"
                     /locus_tag="ACHU0J_001897"
     CDS             758..1768
                     /gene="ruvB"
                     /locus_tag="ACHU0J_001897"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1216608.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1915..2700)
                     /gene="znuB"
                     /locus_tag="ACHU0J_001898"
     CDS             complement(1915..2700)
                     /gene="znuB"
                     /locus_tag="ACHU0J_001898"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1216609.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2697..3452)
                     /gene="znuC"
                     /locus_tag="ACHU0J_001899"
     CDS             complement(2697..3452)
                     /gene="znuC"
                     /locus_tag="ACHU0J_001899"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1216610.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3531..4463
                     /gene="znuA"
                     /locus_tag="ACHU0J_001900"
     CDS             3531..4463
                     /gene="znuA"
                     /locus_tag="ACHU0J_001900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1216611.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4479..5801
                     /gene="mepM"
                     /locus_tag="ACHU0J_001901"
     CDS             4479..5801
                     /gene="mepM"
                     /locus_tag="ACHU0J_001901"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1216612.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5921..6892
                     /gene="lpxM"
                     /locus_tag="ACHU0J_001902"
                     /gene_synonym="msbB"
     CDS             5921..6892
                     /gene="lpxM"
                     /locus_tag="ACHU0J_001902"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1216613.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7023..8465)
                     /gene="pyk"
                     /locus_tag="ACHU0J_001903"
     CDS             complement(7023..8465)
                     /gene="pyk"
                     /locus_tag="ACHU0J_001903"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1216614.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8593..9462)
                     /gene="hexR"
                     /locus_tag="ACHU0J_001904"
                     /gene_synonym="yebK"
     CDS             complement(8593..9462)
                     /gene="hexR"
                     /locus_tag="ACHU0J_001904"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1216615.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9799..11274
                     /gene="zwf"
                     /locus_tag="ACHU0J_001905"
     CDS             9799..11274
                     /gene="zwf"
                     /locus_tag="ACHU0J_001905"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1216616.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11509..13320
                     /gene="edd"
                     /locus_tag="ACHU0J_001906"
     CDS             11509..13320
                     /gene="edd"
                     /locus_tag="ACHU0J_001906"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1216617.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13357..13998
                     /gene="kdgA"
                     /locus_tag="ACHU0J_001907"
                     /gene_synonym="eda"
     CDS             13357..13998
                     /gene="kdgA"
                     /locus_tag="ACHU0J_001907"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1216618.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14054..15232)
                     /gene="purT"
                     /locus_tag="ACHU0J_001908"
     CDS             complement(14054..15232)
                     /gene="purT"
                     /locus_tag="ACHU0J_001908"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1216619.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            15366..15656
                     /gene="yebG"
                     /locus_tag="ACHU0J_001909"
                     /pseudo
     CDS             15366..15656
                     /gene="yebG"
                     /locus_tag="ACHU0J_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            15723..16079
                     /gene="yebF"
                     /locus_tag="ACHU0J_001910"
     CDS             15723..16079
                     /gene="yebF"
                     /locus_tag="ACHU0J_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1216620.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            16406..17065
                     /locus_tag="ACHU0J_001911"
     CDS             16406..17065
                     /locus_tag="ACHU0J_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1216621.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17275..19335
                     /gene="ptrB"
                     /locus_tag="ACHU0J_001912"
     CDS             17275..19335
                     /gene="ptrB"
                     /locus_tag="ACHU0J_001912"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1216622.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(19332..19994)
                     /gene="exoX"
                     /locus_tag="ACHU0J_001913"
     CDS             complement(19332..19994)
                     /gene="exoX"
                     /locus_tag="ACHU0J_001913"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1216623.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20018..20674)
                     /gene="yobB"
                     /locus_tag="ACHU0J_001914"
                     /pseudo
     CDS             complement(20018..20674)
                     /gene="yobB"
                     /locus_tag="ACHU0J_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(20776..21006)
                     /gene="holE"
                     /locus_tag="ACHU0J_001915"
     CDS             complement(20776..21006)
                     /gene="holE"
                     /locus_tag="ACHU0J_001915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1216624.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21145..>21254
                     /locus_tag="ACHU0J_001916"
     CDS             21145..>21254
                     /locus_tag="ACHU0J_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216625.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         5396 a         5289 c         5493 g         5076 t
ORIGIN      
        1 ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg caagttttct
       61 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
      121 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
      181 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
      241 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
      301 aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg ttcaaagagt
      361 tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc ggaatgtcag
      421 cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg aaactgccgg
      481 gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt
      541 tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct gctagcccgg
      601 cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc tataaaccac
      661 aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt gaaacattaa
      721 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
      781 ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact
      841 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
      901 gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa
      961 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
     1021 tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg aaccgcatga
     1081 cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc
     1141 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     1201 taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac
     1261 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     1321 tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg
     1381 tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     1441 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     1501 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     1561 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     1621 agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt tgattcagca
     1681 aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt
     1741 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacac
     1801 tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga gatagcgaac
     1861 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga tagattagct
     1921 ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca ccaccgacgg
     1981 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     2041 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     2101 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     2161 aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt tcacaccatc
     2221 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     2281 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     2341 cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca tcagactaac
     2401 gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     2461 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     2521 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     2581 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     2641 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     2701 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atgattatga
     2761 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     2821 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     2881 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     2941 tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc atttacatcc
     3001 acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa tgctcgcgct
     3061 aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     3121 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     3181 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     3241 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     3301 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     3361 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     3421 gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     3481 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     3541 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     3601 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     3661 atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat gattattcac
     3721 tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     3781 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag caggtaacga
     3841 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     3901 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     3961 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     4021 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     4081 tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     4141 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     4201 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     4261 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     4321 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     4381 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     4441 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     4501 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     4561 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat gccacgccaa
     4621 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     4681 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     4741 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     4801 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct gctgccgaca
     4861 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     4921 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     4981 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     5041 tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
     5101 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
     5161 aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
     5221 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca atccgcgctg
     5281 aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
     5341 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact aatccggtga
     5401 caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt acgccagtgc
     5461 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
     5521 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
     5581 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
     5641 gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
     5701 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
     5761 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
     5821 ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc cgctacacta
     5881 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
     5941 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
     6001 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
     6061 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
     6121 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
     6181 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
     6241 gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg aagagatgcg
     6301 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
     6361 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
     6421 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
     6481 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
     6541 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
     6601 agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg ttgtaccgct
     6661 gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc caccgatgga
     6721 tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag aagtcgagat
     6781 ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga aaactcgcaa
     6841 accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat aaaaaagcct
     6901 ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa tattcgaaat
     6961 ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat ccggcaacgt
     7021 acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact catcacgtcg
     7081 ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg cagcagatta
     7141 accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac cggcgtaacg
     7201 ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc gaaaattggt
     7261 agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt catggtaatg
     7321 atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc aatagcttct
     7381 tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct cgggattttt
     7441 tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata ctgcccagcg
     7501 gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt tgctacgtcc
     7561 atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat ctgggtcgcc
     7621 gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg aatgccgacc
     7681 agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac cacgtcagag
     7741 gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg ttcaaccttg
     7801 gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata gttcagatct
     7861 tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc cgcagtctta
     7921 atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc aagtttgttg
     7981 atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac tttcatgccc
     8041 tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat gtcaccaggc
     8101 acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc gccttcacct
     8161 ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac tttgccttct
     8221 ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc cacatgacgc
     8281 cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc ttcaggcgag
     8341 ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt ttcaagatta
     8401 ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg aagccttttg
     8461 gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga aatccgaatg
     8521 aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag attaggacag
     8581 tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa aacgcgattc
     8641 cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc gtcgcaaagt
     8701 aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa ccatgggcat
     8761 ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt cccgggcgag
     8821 cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg ccagctgcgc
     8881 cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca cgtctccgtc
     8941 gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca ccaccggaac
     9001 attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag agcctaatcc
     9061 gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga tggcagattt
     9121 atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata ttttccccgt
     9181 gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag tgccattcgc
     9241 cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca tgctgcgaca
     9301 gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag caatactcga
     9361 atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct ctgatttgct
     9421 caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa gagactcacg
     9481 ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga tattacgcct
     9541 gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca taacatgatc
     9601 agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg ccgcgctttg
     9661 cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca agctcgtaaa
     9721 agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta ccgggttagt
     9781 taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga cctggtcatt
     9841 ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa
     9901 aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc tgactgggat
     9961 aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa agaaaccatt
    10021 gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct cgatgtcaat
    10081 gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg tatcactatt
    10141 aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct tggcgaggca
    10201 aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac gtcgctggcg
    10261 acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca ggtttaccgt
    10321 atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac
    10381 tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat taccgtggca
    10441 gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca gatgcgtgac
    10501 atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc gccatctgac
    10561 ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct gcgccgcatc
    10621 gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg cttcgcccag
    10681 ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag caatacagaa
    10741 accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt gccattctac
    10801 ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca
    10861 cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa actgactatc
    10921 cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc tggccttgac
    10981 cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac ctttaatcag
    11041 acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca
    11101 ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc cattactgag
    11161 gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt
    11221 gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga gtaatatcag
    11281 cgcttatcct ttatgggtat tttaccggta acatgatctt gcacagattg tagaacaatt
    11341 tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta cactgactga
    11401 aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc tctgaatgaa
    11461 acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat gaatccacaa
    11521 ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc tgcttatctc
    11581 gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc atgcggtaac
    11641 ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa aagcatgttg
    11701 cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca ccagccttat
    11761 gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt
    11821 gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg aatggaattg
    11881 tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc ccataacatg
    11941 tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct gacgatggca
    12001 gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt
    12061 ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc
    12121 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
    12181 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
    12241 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
    12301 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
    12361 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
    12421 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
    12481 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
    12541 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
    12601 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
    12661 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
    12721 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
    12781 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
    12841 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
    12901 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
    12961 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
    13021 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
    13081 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
    13141 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
    13201 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
    13261 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
    13321 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
    13381 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
    13441 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
    13501 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
    13561 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
    13621 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
    13681 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
    13741 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
    13801 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
    13861 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
    13921 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
    13981 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
    14041 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
    14101 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg ctgccatcaa
    14161 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
    14221 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
    14281 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
    14341 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
    14401 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
    14461 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
    14521 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
    14581 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
    14641 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
    14701 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
    14761 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
    14821 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
    14881 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
    14941 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
    15001 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
    15061 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
    15121 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
    15181 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
    15241 tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
    15301 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
    15361 tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt
    15421 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
    15481 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
    15541 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
    15601 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
    15661 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
    15721 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
    15781 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
    15841 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
    15901 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
    15961 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
    16021 ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
    16081 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
    16141 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
    16201 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacctgatat
    16261 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
    16321 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
    16381 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
    16441 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
    16501 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
    16561 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
    16621 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
    16681 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
    16741 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
    16801 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
    16861 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
    16921 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
    16981 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
    17041 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
    17101 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
    17161 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
    17221 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
    17281 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    17341 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
    17401 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    17461 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    17521 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    17581 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    17641 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    17701 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    17761 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    17821 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    17881 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    17941 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    18001 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    18061 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    18121 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    18181 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    18241 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    18301 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    18361 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    18421 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    18481 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    18541 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    18601 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    18661 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    18721 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    18781 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    18841 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    18901 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    18961 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
    19021 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    19081 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    19141 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    19201 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    19261 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    19321 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    19381 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
    19441 gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga cggacgtccg
    19501 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    19561 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag cggtgtctgt
    19621 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    19681 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    19741 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    19801 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    19861 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    19921 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    19981 ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga atgatatcgc
    20041 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    20101 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    20161 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    20221 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    20281 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    20341 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
    20401 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
    20461 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    20521 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    20581 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    20641 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    20701 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    20761 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    20821 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    20881 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    20941 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    21001 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    21061 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    21121 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    21181 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    21241 cagtatcctg cggc
//
LOCUS       DAZPDE010000056        21032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPDE010000056 DAZPDE010000000
VERSION     DAZPDE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.056"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            121..441
                     /locus_tag="ACHU0J_001917"
     CDS             121..441
                     /locus_tag="ACHU0J_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216626.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(507..739)
                     /locus_tag="ACHU0J_001918"
                     /pseudo
     CDS             complement(507..739)
                     /locus_tag="ACHU0J_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(849..1619)
                     /gene="yafV"
                     /locus_tag="ACHU0J_001919"
     CDS             complement(849..1619)
                     /gene="yafV"
                     /locus_tag="ACHU0J_001919"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1216627.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1800..2246
                     /gene="ivy"
                     /locus_tag="ACHU0J_001920"
     CDS             1800..2246
                     /gene="ivy"
                     /locus_tag="ACHU0J_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1216628.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2289..4733)
                     /gene="fadE"
                     /locus_tag="ACHU0J_001921"
     CDS             complement(2289..4733)
                     /gene="fadE"
                     /locus_tag="ACHU0J_001921"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1216629.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4973..5551
                     /gene="lpcA"
                     /locus_tag="ACHU0J_001922"
     CDS             4973..5551
                     /gene="lpcA"
                     /locus_tag="ACHU0J_001922"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1216630.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5757..6524
                     /locus_tag="ACHU0J_001923"
     CDS             5757..6524
                     /locus_tag="ACHU0J_001923"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1216631.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6495..7235)
                     /gene="dpaA"
                     /locus_tag="ACHU0J_001924"
     CDS             complement(6495..7235)
                     /gene="dpaA"
                     /locus_tag="ACHU0J_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1216632.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7619..8140
                     /locus_tag="ACHU0J_001925"
                     /pseudo
     CDS             7619..8140
                     /locus_tag="ACHU0J_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8316..8813
                     /gene="rayT"
                     /locus_tag="ACHU0J_001926"
     CDS             8316..8813
                     /gene="rayT"
                     /locus_tag="ACHU0J_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1216633.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(9030..10742)
                     /locus_tag="ACHU0J_001927"
                     /pseudo
     CDS             complement(9030..10742)
                     /locus_tag="ACHU0J_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10714..11499
                     /gene="lafU"
                     /locus_tag="ACHU0J_001928"
     CDS             10714..11499
                     /gene="lafU"
                     /locus_tag="ACHU0J_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1216634.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11570..12625
                     /gene="dinB"
                     /locus_tag="ACHU0J_001929"
     CDS             11570..12625
                     /gene="dinB"
                     /locus_tag="ACHU0J_001929"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1216635.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12677..12970
                     /gene="yafN"
                     /locus_tag="ACHU0J_001930"
     CDS             12677..12970
                     /gene="yafN"
                     /locus_tag="ACHU0J_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR1216636.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12973..13371
                     /gene="yafO"
                     /locus_tag="ACHU0J_001931"
     CDS             12973..13371
                     /gene="yafO"
                     /locus_tag="ACHU0J_001931"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR1216637.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            13381..13833
                     /locus_tag="ACHU0J_001932"
     CDS             13381..13833
                     /locus_tag="ACHU0J_001932"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1216638.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            14079..15122
                     /locus_tag="ACHU0J_001933"
     CDS             14079..15122
                     /locus_tag="ACHU0J_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1216639.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            15184..15798
                     /gene="prfH"
                     /locus_tag="ACHU0J_001934"
                     /pseudo
     CDS             15184..15798
                     /gene="prfH"
                     /locus_tag="ACHU0J_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(15855..17312)
                     /gene="pepD"
                     /locus_tag="ACHU0J_001935"
     CDS             complement(15855..17312)
                     /gene="pepD"
                     /locus_tag="ACHU0J_001935"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1216640.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            17574..18032
                     /gene="gpt"
                     /locus_tag="ACHU0J_001936"
     CDS             17574..18032
                     /gene="gpt"
                     /locus_tag="ACHU0J_001936"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1216641.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            18124..19368
                     /gene="frsA"
                     /locus_tag="ACHU0J_001937"
     CDS             18124..19368
                     /gene="frsA"
                     /locus_tag="ACHU0J_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1216642.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            19426..19827
                     /gene="crl"
                     /locus_tag="ACHU0J_001938"
     CDS             19426..19827
                     /gene="crl"
                     /locus_tag="ACHU0J_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1216643.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(19866..20921)
                     /gene="phoE"
                     /locus_tag="ACHU0J_001939"
     CDS             complement(19866..20921)
                     /gene="phoE"
                     /locus_tag="ACHU0J_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1216644.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         5129 a         5337 c         5387 g         5179 t
ORIGIN      
        1 aattagaaaa gaacttggta atattcttca gaatatggct aggaataatg aggtgttagt
       61 tcatgggcaa attcttggaa ataaagttag ccgatgtaat tagttagtgg agaggaagtt
      121 gtggacgaaa ttagaataag aattgctggt aatacggctg cgccttgttt ccatgtgcta
      181 aaaaataagg gatataaagt aacaatggaa actcatgcat tatctgacga ttgtttagat
      241 tgtgtatatg attggtatgc ggaaaaaaat ggacaatttt ttagtgcaac gagtccagaa
      301 gagttacttg gattagttgc aatgtgggaa gttcgtggta atgactggag agctaatttg
      361 aatgagataa atacttatca aaaagccata gaagaatccc cgttttttga taaaaacggc
      421 aatgaaatag agtctgacta aattttattg atatagcaac gataataacc ggaaatttct
      481 tccagatctt tccggctttt tattatttaa ctttcgttca gcacactaaa catcccgtct
      541 cgcatcccct taactggctg tttaacctga taaagttccc tgcgcagttc ctgtacttcg
      601 ttgttatccc aagcaggatc aaacatccct ccgagatctt cacggtaacg cccgtgccga
      661 tctcaaatcc cgcttttctc agccatttgg cgctgatttt gatcaccggc gtgtcgctgc
      721 ggttgggcag ataacccacg gtacagcgac gctgtgtttt ggaaatggtg acttctggcg
      781 taatattgtg tttaagttag gggctttcgt aatctagccc agcccccggc cctaaacaca
      841 acccccactc accacaacct aaactcatcc gcatcccgcc atgccggaaa cttttctcta
      901 tattcccgca atgccaccat cgacagctcc gcatcaatac gcgttgcctt atgcgcgtcg
      961 gcggtagcga taatctcgcc ttgcggatta atcacccggc tgtcaccgcg ataatggcag
     1021 ccgttgccat cgctgccgac gcgattgcat cccgccacat acgcctgatt ctcaatcgcg
     1081 cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag ctggccagtt ggcgacgtac
     1141 agggcgaggt cataatcgtt gagattgcgc gaccacactg gaaaacgtaa gtcgtagcac
     1201 accagcggca aaatacgcca gccgcgccat tccacaatca ctcgcgcatt gcccgcttta
     1261 taatgcagat gctcatctgc catgcggaac agatgacgct tatcataaaa atgtaccgtg
     1321 ccgcccggct caaccagcaa aaagcggtta accgaaccag actccgtttg taatgcaaca
     1381 ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg tcatccagtt cactacgtca
     1441 tcttgtgcaa gcgacgaagc tgccgcttcc atggcaaagc cgctggtaaa catctccggt
     1501 agaacgatca catcgcgccc ggtaatacct tccagttgac gatcaaaatg gcgcaggttg
     1561 gcaggaccat ccacccacac caacggttgc tgcaaaagcg taatcttcaa actaggcacg
     1621 gtgtacaact cctttatgcg aagggtttta taactttaac accttatcag gcagttgcct
     1681 tagcgcagaa taaattgata acaaatgctg atattggaaa tatctgattt gcaaattatc
     1741 gtgttatcgc taggctttag gaggttaata acatgggcag gataagctcg ggaggaatga
     1801 tgtttaaggc aataacgaca gtcgccgcac tggtcatcgc caccagtgca atggcgcagg
     1861 atgatttaac cattagcagc cttgcaaagg gcgaaaccac caaagctgcg tttaatcaga
     1921 tggtgcaagg gcataagcta cctgcatggg tgatgaaagg cggtacttat actcccgcac
     1981 aaaccgtaac gttgggagat gagacgtatc aggtgatgag cgcgtgcaaa ccgcatgact
     2041 gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc taatcagatg acggggctgt
     2101 tctcggctat tgatgagaaa acgtcgcaag agaaactcac ctggctgaat gtgaacgatg
     2161 cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt gaccggcagc ctggaaaacc
     2221 atccggatgg ctttaatttt aaataattag cggataaaga aacggagcct ttcggctccg
     2281 ttattcaatt acgcggcttc aactttccgc actttctccg gcaactttac cggcttcgtc
     2341 gccagctctt ccggatcaaa gtcatcaacg ttaatactgc gcagacggct ttcttccgct
     2401 ttcaccagaa tagcggcttc atctttatca atcagcccct tcgtcagcgc gttgtgcgcc
     2461 agttcatcca gacgggtaaa tggcagattt ttacccagct ctttacagat ccgctgatga
     2521 atcgggtcgg cggcaatcac atccaccaac gcctcttcca gtaaaccaac cgggttatgc
     2581 tcgcttggcg tcaggtactg accgcgacca atacgtgaac gagtggcgtt cggcacttgt
     2641 aaaatcttcg ccattttatg atccagcttg tcagacggtg ccagataatg acgtccggtc
     2701 gggaagatca ccacattcag cagcccggca accacgcggt tcgggaagtt ttgcagtaaa
     2761 ttgtccattg cctgttctgc ctgatacagc gcatcctgta cgccccagtg caccagcggc
     2821 agatcggctt cattacggcc ttcgtcgtca taacgcttca gaacggcaga ggcgaggtaa
     2881 agctggctta aaatatcccc cagacgtgcc gagatacgct cacgacgttt caggctgccg
     2941 cccagcagtg ccatcgagac atcagaaagc agggcgaggt tggcgctcag gcggttcagg
     3001 tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc tgctggttaa accgcgcgtc
     3061 aggcccagcc agaagctgcg aactttgttg ctaccgacgt gaccgatatg tttgaacaac
     3121 agtttatcga acgcgttgac gtcattgttc ttcgccgctt ccatctcttc cagcacgtac
     3181 ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc tgcgggtcag aatgttagcc
     3241 ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac gcgccaggaa gttgctttgc
     3301 ccgagcataa tgcctttacc gccggtaata tccatcgcat caataatcga ctgctgcccg
     3361 cggtgggtac agtgatactt aacgatagcc gacagcacgg caggtttttc gccgagcata
     3421 atgccgtagg taatcagcga tgccgcagca tccattacat aagcgttccc ggcaatacgc
     3481 gccagcggct cttcaatccc ttccatctta ccgatagaga ttttgaactg acggcgaatg
     3541 tgagaatacg cgcccgttgc cagcgctacc gatttcacgc cgccggttga gttggaaggc
     3601 agggtgatgc cgcggcctac cgagaggcac tccaccagca tccgccagcc ttgcccggcc
     3661 attttcggcc cgccgatgat gtaatcgatc ggcacgaaga catctttacc gcgcgtcggt
     3721 ccgttctgga acggtacgtt cagcgggaag tggcgacgac caatttccac gcccggcgtg
     3781 gtggttggga tcagcgcaca ggtaatgcct aaatcttctg caccgccgag taatttttcc
     3841 gggtcggaga gtttaaacgc cagcccaagc acggtcgcaa tcggtgccag cgtaatgtag
     3901 cgtttgttcc aggtcagacg catccccagc acctgctggc cctgccattc gcccatgcag
     3961 acaatcccgg tgtccggaat cgcgcccgca tcggaacccg cttccgggct ggtcagtgca
     4021 aagcagggga tctcctgacc acgcgccaga cgcggcagat agtgatcttt ctgctcgtca
     4081 gtgccgtaat gttgcaacag ttcgcccggg cctaatgagt ttggcacgcc gacggtaatc
     4141 gccaggatcc cgctcacgcc agagagtttt tgcagcacac gagactgggc ataagctgag
     4201 aactccagtc cgccgtactc ttttttgata atcatcgcga agaagcgatg ctctttaagg
     4261 tacgcccaca actccggtgg cagatccgcc agctcatgag taatctggaa atcattcgcc
     4321 atccggcagg cttcttctac cgggccgtcg agaaacgctt gctcttcagc ggtcaggcgc
     4381 ggctgcggat agttatgcag ctttttccag tccggcttgc cctggaacaa gtcgccctcc
     4441 caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc gcgacatcgg cggcatcacc
     4501 ttacggaaac cgcgaaatac cggcgcggaa atcatcgact tacgcatagg cgcaaagtta
     4561 aatggcacga ggataatggt cagaggcacc agtacccacg ccgaccacag accagcaacg
     4621 ccgagggcgg ctgtccaggc gagcaaaatc agactgctga taaataagct cacgcggtga
     4681 tagaacaacg cgccgagcag gacaaccgta gcgagaatac tcaaaatcat cataacgaaa
     4741 agccccttac ttgtaggagg tctgaccact tgtgatgata tggttgtagt ggatgtaaaa
     4801 acatttagca atgtgtttac aatataatta caacaaagct cacattgttg ctgtttttat
     4861 ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc gcttttggcg
     4921 gtatccggta cactgcattt tgtctattac atttatgctg aaggatatcc tcatgtacca
     4981 ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact ttttaaaaga
     5041 tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca gctttaaagc
     5101 cggtggcaaa gtgctttcct gcggcaacgg cggttcccat tgcgacgcta tgcactttgc
     5161 cgaagagttg accggtcgct accgtgaaaa ccgtccgggc tacccggcga ttgctatttc
     5221 tgacgttagt catatttcct gcgtcggtaa tgatttcggt ttcaatgata ttttctcccg
     5281 ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg gggatctcca cctccggtaa
     5341 ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga aagtgatcac
     5401 cctgaccggt aaagacggcg gtaaaatggc tggcacggcg gatatcgaaa ttcgcgttcc
     5461 gcactttggt tatgccgacc gcattcagga gattcacatt aaagtgatcc atatcctgat
     5521 ccagttaatt gaaaaagaga tggttaagta agtctggcgt aggccggata aggcgtttac
     5581 gccgcatccg gcacttgtgc gctgatgcct gatgcgatgc tgacgcgtct tatcatgcct
     5641 acaaatctgt acccgaaccg taggccgaat aattcgttta cgccgcatcc gacctgaaaa
     5701 ttcttaaatc aatcttcgcc ggggtccatg cgctcccgct gttgtggagg ttacccatgt
     5761 gcgaattgct cgggatgagc gccaacgtcc ctaccgatat ctgctttagt ttcaccggac
     5821 ttgtacagcg tggtggtgga accgggccac ataaagatgg ctggggcatt accttttacg
     5881 aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag ctttaattcc cccatcgcca
     5941 aacttgtcca ggactacccg ataaaatcct gttcggtggt ggctcatatt cgccaggcta
     6001 atcggggcga ggtggcgctg gaaaatactc acccatttac ccgcgagtta tgggggcgta
     6061 actggactta tgcccataac ggacaactga cgggctacaa atcactggaa accggcaact
     6121 tccgcccggt cggtgaaacc gacagcgaaa aagccttttg ctggctgctg cataaattaa
     6181 cgcagcgtta cccgcgcacg ccgggcaaca tggcggcagt gtttaaatat atcgcctcac
     6241 tggcggatga actgcgggcg aagggcgttt tcaacatgct gctttcggac gggcgctatg
     6301 taatggcgta ttgctcgact aatttacact ggatcacccg ccgcgcgccg tttggcgtgg
     6361 caacgttgct ggatcaggat gtggaaatcg acttcagctc gcagaccaca ccgaatgatg
     6421 tggtcacggt gattgcgaca cagccgctga cgggcaatga aacctggcaa aaaattatgc
     6481 caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag ttgtggctgc
     6541 accacttcgt ggcttaacgg cttgctgacc acgtaacggc cattggccac agaaacggtc
     6601 ggtggcttac gggtttgctc aaagtagtcg tagcccggct tcagctgctc ccagaagtcc
     6661 ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa tgggtaaata
     6721 ctcacctgca cgctcggctg accaaacacc agcgcaccag taacgaactg gaagatctca
     6781 tcaataccct gattggtcat tgcgtagcag ccgatggata cacaatcgcc gtggatcatc
     6841 aggtatttcc cttcataacc atgcgcacgg tcataagcat tggggaatcc aatattaatc
     6901 gctttgtagt aacggctgtc tggttttaac tgattacgct ggacgctata aaacccttcc
     6961 gggcttttga aatcgccctg acgctgtttt ggccctaagc cgccggaata tttacagatt
     7021 ttataactgt cgagcagttg atattgctcg cccattttga cgtaaagatc gagcgtacgt
     7081 tcttccttga agatctggat gtagacaggg gatcccatca actgctgctt atactctttg
     7141 ctcactggcg tggtcgagct actgctgccc agcaaaccgg caaacgaaac gcacgggatc
     7201 aacaacatcg caagaattaa tgcgatttta cgcatactgc ttattccttg ataaaacggt
     7261 tacacacgcc aggacggcaa aatggatccc aaatcggaat agtctggatt tggaaggctc
     7321 acattatcac caaaagagtt ttacgcaagc ctgtcggcgc tgggtttaca aatttagcac
     7381 aggggcagtt ttcaatagtc cgaaaagcgc atgtatcatt tatttcctaa tattatgaaa
     7441 tctcaatagt aatatgcaaa taagcaatta ataatcccgt ttactcctcc ggtcttttaa
     7501 aattctcacc ttcattcagc cggtttatgt ctttcatgtc ttcgttcttg ctcggcagat
     7561 ttttgcatcc aggcgtgttt tcgttatgcg ttttgctccc tttactcgcc agcgccacca
     7621 caaccgggag cagataaaac agcatccaga atggtttcct gcgccgctca aggcgagtga
     7681 cagacgctgg caggcgctgg cggaaaacaa ccacttttta agcagcgatc acctgcataa
     7741 catcaccgaa gtggcgattc accgcctgga gcagcagctt ggcaagcctt acgtctgggg
     7801 cggcacgcgg cccgatcaag gcttcgattg tagtgggtta gttttttatg cctacaacaa
     7861 gatccttgag gcgaaactcc cgcgcacggc caatgagatg taccactacc gtcgggcaac
     7921 gattgtggcg aataacgacc tgcgccgggg agatttgcta tttttccata tccacagccg
     7981 cgagattgcc gaccatatgg gggtgtatct gggcgacggt cagtttattg agtcactgcg
     8041 caccggggaa accattcggg taagccggtt agccgaacct ttctggcagg accatttttt
     8101 aggcgcgcgc aggattttga cggaagagat gattttgtag gccggataag gcgtttacgc
     8161 cgcatccggc ggttgtgcac ggatgcctga tgcgacgctg acgcgtctta tcatgcctac
     8221 aaatctgtgc gtttttgcga gccgctttcc cgatataaca cagcgtcatt gtcggccttt
     8281 ccttttcctc ttaccgttga gaaaaaggag tcgctatgtc ggaatatcgt cgttattaca
     8341 tcaagggggg aacatggttt ttcacggtaa atttacgcaa tcgtcgaagc catcttctga
     8401 ccacccagtt ccagacgctc cgtaacgcca ttattaacgt taagcgagac agaccttttg
     8461 aaattaacgc ctgggtcgtt ttgccggagc atatgcattg catatggaca ttacccgaaa
     8521 gcgatgatga tttttcctct cgctggcggg agattaaaaa gcagttcacc catgcttgcg
     8581 gattgaaaaa tatctggcaa ccgcgttttt gggagcatgc cattcgcaat acaaaagatt
     8641 atcgccatca tgttgattat atttatataa atccagagaa acatggctgg gtaaagcagg
     8701 tgagtgattg gccattctca acgttccatc gcgatgtcgc gcgagggtta tatcccatcg
     8761 attgggcggg agacataacg gatttaagtg ccggggagcg tatcatttta taactgtgcg
     8821 ctggtgccgg atgcgcttac gcttatccgg cctacaaatc gtgctttaac ggtaggccgg
     8881 ataaggcgtt cacgccgcat ccggcagtta tgcgcagatg cctgatgcga cgctggcgcg
     8941 tcttatcatg cctaaaaact tgtgcctgaa cggtaggccg gataaggcgt ttacgccgca
     9001 tccggcaatt gtgcaccgat gccggattct tacatcaacg cccccataat cttcaactcc
     9061 agctcatccg gcacttcgtt atacgacagc acatgcagcc ccggcgcaaa caaccttgca
     9121 taacgcggca gcaaagggcg tagctgcggc ggcaccagca gcaccggatc tttccccgcc
     9181 gctttcatct gctctttcac ctgtggcatc gtgctctgga actggttgag catattcggg
     9241 tcaaccggca cgctgtcgag catcactttg ccggcctgtt gcgcctgatt cactacgttg
     9301 gtcagcagat tttccagctc attattcagc gtatacaccg tcagctcctg cttgcgaacg
     9361 aacggatgag taatgctgcg ccgcagcgcc aggcgcacat cggccgccag cagaatatga
     9421 tctttagtca ccgtgctact ggcgaccagc acggtggcga tggtgacgat atcgcgcagg
     9481 gaaacgcctt cggtcagcag cgcccggtac actttcagca attggctgta attgagcgcc
     9541 gcgcttaaat cctccgccag gcgcggtgcc atcgacgaca aacggttatg caactgcgtg
     9601 atgtcatcat agttaaacaa atcaggaata tagctgcgca caatcttgtt cacatgggtg
     9661 gcaatcacac tggcgctgtc gatcacctga taccccatat tcagagcctt cgctttctgc
     9721 gcaggctgga tccaggtgac cggtatcccg tacgccgggt cgttccccag cacgccgtca
     9781 atctcgccgt aggtttcgct ggagggcagc gccatcagtt tatccgccgg aatatccgct
     9841 tcatcagcct caatgccgtt gatgaaaata gcgtactggc tgggcttgag gcggaagttt
     9901 tcccgaatgc ggatctccgg cagcagcacg ccgttaccgt cggaaatcac ctgccgaacg
     9961 ccgcgaatcc gctgggtgag cgggttgccc tgcgctttgt ccaccagcgc caccagcttg
    10021 tagccgaggc ttaagctgat gggctcgatc agcggaatag tttcccaact gacctgttgt
    10081 tcgctggttt cagtgatggt gcgggtcagc gtttcgaggc ttttctcttc cgcctccgcc
    10141 gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa gcagggcgct gaacagcaaa
    10201 aacggcagat gcggcatccc cggcaccacc gccagcacga acatgatccc ggtggcggtg
    10261 tagagcaccg acgggcttgc cagcaactgg tggcgcacgt catgggcgat atcgccgttg
    10321 tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga gcagcaggga agggatctgc
    10381 gccaccaggc cgtcgccgat ggtcatcagc acgtattgct ggaaggcggc gtcggcgctc
    10441 aggttgtatt tgaagatgcc gatacagacg ccgccgctca ggttgatcgc cagaatcatc
    10501 atcccggcga tggcgtctcc gcgtacaaac ttcgatgccc cgtccatcgc gccgtagaaa
    10561 tcggcctcgc tggcaacatc tttacgccgg gtttgcgcct gcgcctggtt gatcaatccg
    10621 gcgttaagat cggcgtcaat cgccatctgt ttgccgggca tcgcgtccag ggtaaagcgg
    10681 gcagaaacct cggaaatacg ctcggcctct ttggtgacga cgataaagtt gattgtcaac
    10741 agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc tgcacgggca gtcgattttt
    10801 aacggtggtg ggctgtcgcc gttaaataaa atcagcccgt ctcatccgcc aaaacctgcc
    10861 accgtggcgg tgccggaaga gacggagaaa aaagcgcgtg atgtgaatga gaaaacggcg
    10921 ctgctgaaga agaaatccgc caccgagctt ggcgagttgg caaccagtat caacaccatt
    10981 gcccgtgatg cgcatatgga agcgaatctg gagatggaga ttgttcccca gggattacgc
    11041 gtgctgatta aagacgacca gaatcgcaat atgttcgaac gcggcagcgc gcagattatg
    11101 ccgttcttta aaactttgct ggtggagctc gcgccagtgt tcgactcgct ctataacaaa
    11161 attattatta ccgggcatac cgatgcgatg gcctacaaaa acaatatcta caacaactgg
    11221 aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc tggaagaggc cggaatgccg
    11281 gaagataaag tgatgcaggt aagcgcaatg gcggaccaga tgctgctgga cgcgaagaat
    11341 ccgcaaagcg cgggcaatcg gcgtattgag attatggtgc tgaccaaaag tgcgtccgat
    11401 acgctgtatc aatactttgg tcagcatggg gataaagtgg tgcagccgct ggtgcaaaag
    11461 ctggataagc agcaggtgct ttcgcagcga atgcgctaaa tgctgaatct ttacgcattt
    11521 ctcaaaacct gaaatcactg tatactttac cagtgttgag aggtgagcaa tgcgtaaaat
    11581 cattcatgtg gatatggact gctttttcgc agcggtggag atgcgcgaca atcccgctct
    11641 gcgcgatatc cctattgcta ttggcggcag ccgcgaatgt cggggggtga tcagcaccgc
    11701 caattatccc gcgcgtaaat ttggcgtacg tagcgctatg cctactggga tggcgctcaa
    11761 attatgccca catctcacct tgcttccggg gcgctttgac gcctacaaag aagcctcaaa
    11821 tcatatccgt gaaatcttct cgcgctacac ctcgcgcatt gaaccgttgt cactggatga
    11881 ggcttatctc gatgtcaccg atagcgtcca ttgccacggt tctgcgaccc tcatcgccca
    11941 ggaaatccgc cagacgatct tcaacgagct gcaactgacg gcatctgcgg gcgtggcacc
    12001 agtaaagttt ctcgccaaaa tcgcctccga catgaataaa cccaacggtc agtttgtgat
    12061 tacgccggca gaagttccgg catttttaca aaccttacca ctggaaaaaa tccccggcgt
    12121 cggcaaagtc tcggcggcaa aactggaagc gatggggctg cggacctgcg gtgatgtaca
    12181 aaagtgtgat ctggtgattc tgcttaaacg ctttggcaaa tttggccgca ttttgtggga
    12241 gcgtagtcag gggattgacg agcgcgacgt taacagcgaa cggttacgaa aatccgtcgg
    12301 cgtggaacgc acgatggcgg aagatattca tcactggtct gaatgtgaag cgattatcga
    12361 gcggctgtat ccggaacttg aacgccgtct ggcaaaggta aaacctgatt tactgattgc
    12421 tcgccagggg gtgaaattaa agttcgacga ttttcagcag accacccagg agcacgtctg
    12481 gccgcggctg aataaagctg acttaatcgc caccgcgcgt aaaacctggg atgaacgccg
    12541 cggcgggcgc ggtgtgcgtc tggtggggct gcatgtgacg ttgcttgacc cgcaaatgga
    12601 aagacaactg gtgctgggat tatgatgtat actattatgt atattctggt gtgcattatt
    12661 atgagggtat cactgtatgc atcgaattct cgctgaaaaa tcggtcaata tcactgagtt
    12721 acgtaaaaac ccagctaaat actttattga tcaaccagtt gcggttcttt ctaataatcg
    12781 ccccgcagga tatctcttaa gtgccagcgc attcgaagcg ttaatggaca tgcttgctga
    12841 acaagaggag aaaaagccca taaaggcgcg ctttcgtcca agtgctgcaa gattagagga
    12901 aattacacgc cgcgctaaac aatatcttaa tgatatgacg gatgatgatt tcaatgactt
    12961 taaggaataa ggatgcgggt attcaaaaca aaacttattc gcctgcaact tacagcagag
    13021 gaacttgatg cgttaacggc ggattttatt tcctataagc gtgacggtgt tttgccagat
    13081 atatttggtc gcgatgcact ctacgacgac tcgtttacct ggccattaat caaatttgag
    13141 cgagttgctc atattcatct ggcaaatgtg aataatccat ttccgccaca gttgcgccaa
    13201 ttcagcagaa cgaatgacga agcgcatttg gtatattgtc agggtgcgtt tgatgagcaa
    13261 gcatggttgc tcattgccat tctgaagcct gaacctcata aactggctcg agataacaac
    13321 caaatgcata aaattggaaa aatggcagaa gcgtttcgca tgcgtttttg aatttatatt
    13381 atgaataaca tacaaataag agattatcag cctggcgatt ttcagcaact atgcgctatt
    13441 ttcattagag cggttacgat gaccgtcagt cagcattatt cactacaaca aattgccgcc
    13501 tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg cgaaatcaca agtgcgggtt
    13561 gcagttatta atgcaaaact ggtcggcttt attacctgcg ttgaacacta tatcgatatg
    13621 ttatttgttg accctgaata cacccgccgt ggggttgcca gcgctttgtt aaaacctttt
    13681 attaagtctg aatccgaact tacggtggac gcaagcataa ccgcaaaacc cttttttgaa
    13741 cgttatggtt ttcagacagt taagcagcag cgcgttgaat gccggggagc gtggtttatt
    13801 aatttttata tgcgttataa accgcaacat taaatccagc ttgcaatgaa aataacgccc
    13861 gcctagtatg tgcgcgtttt cttattccac aaactgcaag gaggtaaatc atgacaaatc
    13921 ctttatcaat gactctttgc agacctttcc aggattaatt ctttttttct tgccctggat
    13981 tcgtctgcca tttcctgatt ttttaattgt tactctcaat aattcaagtc gcagcacatg
    14041 caacttgaag tatgacgagt ctatttacgg aatggtatat gggcaattat attcgtccct
    14101 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
    14161 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
    14221 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctccgcaggc cgtttttacc
    14281 cagcactggt cggcaatgat atcggctgtg gcatggcgct atggcaaaca gatattctcg
    14341 ctcgcaaata caacgccgat aagtttgaaa agcgattatc agctttggat gacgttgctg
    14401 aagaaagctg gctggaggaa aacctgccgt cagcatttgc acagcatccg tggcgcagct
    14461 cgcttggctc tatcggtggt ggcaatcatt tcgctgaact gcaacaagtt gatcaaatca
    14521 tcaacgctga actgtttgca ctggcaggtc tggatgcgca gcatctgcaa ctgctggttc
    14581 atagcggctc gagggggcta gggcagtcta ttttacagcg gcatattgcc tcgttttcgc
    14641 atcatggttt gcctgaaggc agtgacgacg cgctggcgtt tgcgcgtttt aatcgccacc
    14701 tgatcgcttt gcgcataatg caacaggtta aggccaccgg aagtccggtt ctggatgtgg
    14761 cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca aggggtctta catcgtaaag
    14821 gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg ttcacgcggt gattactcca
    14881 ggctggttca gcccgtcagg agtgaggaaa cattgcattc gctggcgcat ggggctgggc
    14941 gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc gaaatacaca gcgacgcaac
    15001 tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga taaacaactc atcttcgaag
    15061 aagcgccaca agcctataaa tcggctgaaa gtgtggtgca atgtcagggc aggccggatt
    15121 aattattcct gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa
    15181 aggatgatct tgctataact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg
    15241 aaaaaggcgc tggacaggct aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg
    15301 ctggaaacag aaacaggccg ctactctgac acgctgcgtt cggcgctgat ttctctggat
    15361 ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca ctattcagtg gatttgcccg
    15421 agcccgtatc gcccacatca cgggcgtaaa aactggtttc tgggcattgg gcgttttacc
    15481 gctgatgagc aggagcaatc cgatgcgatc cggtatgaga cgctgcgttc gtcggggccg
    15541 ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt
    15601 attagcgtga aggttcagtc agagcgcagc cagcatgcta acaaacggct ggcgcgattg
    15661 ctgattgcct ggaagctgga tcagcagcaa taggaaaata gcgcggtgct gaaatcacag
    15721 cggcgaatgt tccatcacca gattgaacgt ggcaacccgc gacgaacgtt tacagggatg
    15781 gcttttatcg aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc
    15841 agcaaatcaa ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag
    15901 taatgaccta cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt
    15961 ggcccgatag aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg
    16021 cccgcgtgga taatctggat gttcggcgtc ttgttgaaca ggcgctgata ggtttcacgt
    16081 accagatgca tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct
    16141 tcggttttcg cgccagccag tttacccagc gaatccagca tgctcaccac gtagtcttta
    16201 ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta cgttattgtc agtcatggtc
    16261 accacaccga cgttcaggga ggtttcgacc acaccttttg ccacgtcgga attgcggatc
    16321 acaccgttcg gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg
    16381 gcagctttat cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc
    16441 agctcgtttt tcaggatctc ctgataggta ttcaccagag atttcagtgc gtcgacttta
    16501 tcagctgcga cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg
    16561 ccgttgaaat cgataaggcg caggtccagt tcttctgcat gacccgccag gaagcgcacc
    16621 agcagtttgt tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc
    16681 agacctttta aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa
    16741 tgcaggttgg aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct
    16801 tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca
    16861 ccgtccatac cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg
    16921 ttttcgtcag ccagaaccgc cagtgcagag gccataccaa tgccgttatc cgcacccagc
    16981 gtggtgccgc gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg
    17041 aagtcatgca cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg
    17101 accggtttac gattttccat acctgcggta gcaggttgac gaatcaggat attacctacc
    17161 tgatcgcgtt cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt
    17221 tgctcttcat gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc
    17281 ggctgtggag ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa
    17341 aacagtattg caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga
    17401 gatatgtatt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    17461 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    17521 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    17581 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    17641 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg
    17701 gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
    17761 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
    17821 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgctgttgcg attcgtgaaa
    17881 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
    17941 atgactatgt tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg
    18001 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ttgccgggcg
    18061 ttgttctttt taacttcagg caggttacaa tagtttccag taagtattct ggaggctgca
    18121 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
    18181 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt
    18241 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
    18301 ggtattgacc cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa
    18361 cgtaccgacg atgatttata cgataccgtg attggctacc gtggtggcaa ctggatttat
    18421 gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct gtacggaaga agatccgcaa
    18481 ctcagtggtc gacactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat
    18541 ctgaaaggag atgacctggc cgagcaagcg caggctttat caaaccgcgc ctatgaagag
    18601 gccgcccagc gtctgccggg cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg
    18661 cccatcaccg gctttttgca tatgccgaaa ggcgatggcc cgttcccgac agtattaatg
    18721 tgtggtggtc tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg
    18781 ccgcgcggca ttgcgatgct gactattgat atgccgtcgg tgggcttttc ttcaaaatgg
    18841 aagctcaccc aggactccag cctgttgcat cagcacgtct taaaggcgct gcctaacgta
    18901 ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc
    18961 gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta
    19021 gttcataccc tgttgagtga ttttaagtgc cagcaacagg tgccggaaat gtatcttgac
    19081 gttctggcga gtcgtttggg gatgcatgat gcttccgacg aagcgttacg cgtggagctg
    19141 aatcgctatt cattaaaagt gcaaggattg ctggggcgtc gctgcccaac gccaatgtta
    19201 tcaggctact ggaagaacga tccgttcagc ccggaagagg actcacgctt aatcacctca
    19261 tcatctgctg acggtaaatt attagagatc ccatttaacc cggtgtatcg gaattttgac
    19321 aaaggtcttc aggaaatcac cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa
    19381 ttttgccaat ttggtaaaac agttgcatca caacaggaga tagcaatgac gttaccgagt
    19441 ggacacccga agagcagatt gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa
    19501 ggtaagtgcg aagataatcg attctttttc gattgtctgg ctgtatgcgt caacgtgaaa
    19561 ccggcaccgg aagtgcgtga attctggggc tggtggatgg agctggaagc gcaggaatcc
    19621 cgttttacct acagctacca gtttggtctg ttcgataaag caggcgactg gaagagtgtt
    19681 ccggtaaaag atactgaagt ggttgaacga ctggagcaca ccctgcgtga gtttcacgag
    19741 aagctgcgtg aactgctgac gacgctgaat ctgaagctgg aaccggcgaa tgattttcgt
    19801 gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat
    19861 attcattaaa actgatacgt catcccaacc gcgacagtat catcattatt aatattcagc
    19921 ttattatcgc tatccagttg gttgatttta taatcaacaa acgctgacat atttttgttg
    19981 aaataatacg tagcaccgac gtcgatataa ttgaccagat cttcatcacc gatgccctca
    20041 atatctttcc ctttcgataa gacataaccc agcgatggac gcagaccaaa gtcaaactgg
    20101 tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa agccgccagt tattggcgtc
    20161 attttgcgtg tttcagaata gaaagttgcc agataaatat tattggcatc gtatttcaga
    20221 cctgttgccc atgcttctgc acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg
    20281 gtgcgatctg agttggtata ggccccacta atggcgaaat cgctgccgcc aaagtcatat
    20341 gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc
    20401 ccttgatatt gcagggttaa gttcaggcca tcgataacgc cgaagaagtc ggtgttccga
    20461 tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt cggtctgcgc cgaggaatcg
    20521 ccaccaaatt ccgggaacat atcggtccag gcttccacgt catacaacgc gcccaggtta
    20581 cgaccataat cgaaagaacc caaatcttta tatttcaacc cggcaaaagc gagacgcgtt
    20641 ttttgctgtg cagtatcact ctccgcttta tttccggcaa actccgcttc ccaccgacca
    20701 taaccagtca gttgatcgtt aatttgagtt tcacctttaa aaccaaaacg gatataactc
    20761 tggtcgccat ctttactggc gttatcactc atataatgca tggctttaac tttgccatag
    20821 acatccagtt tattaccgtc tttattatat atttctgcag cctgtacaga tgcagatgcc
    20881 acaatgccca tcaccactaa tgccagagtg ctctttttca ttttcattcc tgattttaat
    20941 taacgcgcga atattcagcg ggagagcccc gttgaaaaca ggaaagtttt taacctgaga
    21001 ttgttaaaga tatattacag attaatgata tt
//
LOCUS       DAZPDE010000057        20877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPDE010000057 DAZPDE010000000
VERSION     DAZPDE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.057"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..100)
                     /locus_tag="ACHU0J_001940"
     CDS             complement(<1..100)
                     /locus_tag="ACHU0J_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216645.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYL"
     gene            complement(245..4705)
                     /gene="mukB"
                     /locus_tag="ACHU0J_001941"
     CDS             complement(245..4705)
                     /gene="mukB"
                     /locus_tag="ACHU0J_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1216646.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4705..5409)
                     /gene="mukE"
                     /locus_tag="ACHU0J_001942"
     CDS             complement(4705..5409)
                     /gene="mukE"
                     /locus_tag="ACHU0J_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1216647.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5390..6712)
                     /gene="mukF"
                     /locus_tag="ACHU0J_001943"
     CDS             complement(5390..6712)
                     /gene="mukF"
                     /locus_tag="ACHU0J_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1216648.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6709..7494)
                     /gene="cmoM"
                     /locus_tag="ACHU0J_001944"
     CDS             complement(6709..7494)
                     /gene="cmoM"
                     /locus_tag="ACHU0J_001944"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1216649.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7630..8409
                     /gene="elyC"
                     /locus_tag="ACHU0J_001945"
     CDS             7630..8409
                     /gene="elyC"
                     /locus_tag="ACHU0J_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1216650.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8386..9279)
                     /locus_tag="ACHU0J_001946"
     CDS             complement(8386..9279)
                     /locus_tag="ACHU0J_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1216651.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9433..10179)
                     /gene="kdsB"
                     /locus_tag="ACHU0J_001947"
     CDS             complement(9433..10179)
                     /gene="kdsB"
                     /locus_tag="ACHU0J_001947"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1216652.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10176..10358)
                     /gene="ycaR"
                     /locus_tag="ACHU0J_001948"
     CDS             complement(10176..10358)
                     /gene="ycaR"
                     /locus_tag="ACHU0J_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1216653.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10410..11642)
                     /gene="ycaQ"
                     /locus_tag="ACHU0J_001949"
     CDS             complement(10410..11642)
                     /gene="ycaQ"
                     /locus_tag="ACHU0J_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1216654.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11679..12665)
                     /gene="lpxK"
                     /locus_tag="ACHU0J_001950"
     CDS             complement(11679..12665)
                     /gene="lpxK"
                     /locus_tag="ACHU0J_001950"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1216655.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12662..14410)
                     /gene="msbA"
                     /locus_tag="ACHU0J_001951"
     CDS             complement(12662..14410)
                     /gene="msbA"
                     /locus_tag="ACHU0J_001951"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1216656.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14447..16706)
                     /locus_tag="ACHU0J_001952"
                     /pseudo
     CDS             complement(14447..16706)
                     /locus_tag="ACHU0J_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16913..17197)
                     /gene="ihfB"
                     /locus_tag="ACHU0J_001953"
     CDS             complement(16913..17197)
                     /gene="ihfB"
                     /locus_tag="ACHU0J_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1216657.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17357..19030)
                     /gene="rpsA"
                     /locus_tag="ACHU0J_001954"
     CDS             complement(17357..19030)
                     /gene="rpsA"
                     /locus_tag="ACHU0J_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1216658.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(19141..19824)
                     /gene="cmk"
                     /locus_tag="ACHU0J_001955"
     CDS             complement(19141..19824)
                     /gene="cmk"
                     /locus_tag="ACHU0J_001955"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1216659.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19997..20761)
                     /gene="ycaL"
                     /locus_tag="ACHU0J_001956"
     CDS             complement(19997..20761)
                     /gene="ycaL"
                     /locus_tag="ACHU0J_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1216660.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         5033 a         5767 c         5070 g         5007 t
ORIGIN      
        1 cgaggtaatc ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat
       61 caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt
      121 acacagccac tggatcggtc aactgatcct taactgatcg gcattacgcc gttatgtgcc
      181 atttttttag tgcagaaacg gagttttcgg aaaaagaaaa ggcggcattg ctgccgcctt
      241 aattttaact cgcctgggaa ggcgcttcgt ctcttcctag aagcgtttca gggagttgcg
      301 gcgcaaatcc tcgcaggccg acgacgtgaa catgttcggt attctggaag actttacgta
      361 ccagtttata ggtggtgcct ttttccggac tgatattttc cggcgctgcg atgatgagct
      421 gcatttgcaa acgttcacac aattcaaaca gcgtggcgat agaacgagca tccagtcgcg
      481 ctgcttcatc gaggaacagc aggcggcaag gagagatatc tttaccgcgc aggcggcgag
      541 attcatcttc ccagctttgt accaccatca ccagaatcga cataccggta ccaatcgcct
      601 caccggtcga caatgcacca gactctgcgc gcaaccaacc atcggaacca cggttaacct
      661 caacttccat ttccagatag ttgcggtaat ccagcagttc ttcgccaatg gtctgcggcg
      721 tgcgctgccc catatcaatc tgcggattaa gacgctgata caatttcgcc aacgcttccg
      781 agaaggtcag acggttgctg ttaaacagat cctgatgctg ctcgtgctgt tcagagagca
      841 catccagcag catggcgtgc gtttcacgca cgttcacgtt gagacgcacg ctgttcacct
      901 gaccaaacga tacgttctgc aacccctggt tgagcatacg gatacggttc tgctcgcgct
      961 gaatggtttt gcgaatgatg ttcgccacgc tgcgggaact gatcgccagt ttctgttcac
     1021 gggaggttaa ttcttcggtc agacggctaa gttcaatctc catctgttcg atagcttcca
     1081 ccggatcatc ggtacgaata atatcctgac gaatacgttc acgcagatgc tgataaaccg
     1141 ccacgaagaa ctgaatttta cgctccggac gtttcggatc ttccgacatg cgcagcacgt
     1201 cgcgcagatg ttcgttatcc gccaccgcca gacgcagcgc acctaacgcc ttatccgaca
     1261 tggaacgcaa atcatcagcg gagagataag ccagctcacg acggtgtaag cggcgctcaa
     1321 cgccgttatc tttcaccatg cgcatcaccg cacaccagcc cgctttggcg gtcactacct
     1381 gctcgcgcat ctcaaagtaa tcccgctcca gcttgcgcag tttgcgggtc aggttgtcca
     1441 tctccgcttc gcagaaggta agcgcttttt ccagttgatt gcggcgtgaa cggttattgc
     1501 tcagttgcgc atgcagctcg tcacggcgaa tacgcgcccg ctcttctgcc ccgctatcag
     1561 cacgcacgcc gatatcctgc aattcacgtt gcagatcgtt gagtagctct tttttggtgt
     1621 cgtaagaact tttcagcgaa gccagcacct ggttgtactg actcaactgc gctgcgtgac
     1681 cgcgcaacgc ttcgcgagcg cgggtacgct ccgcttccgc ctgttccaga cgttcacgca
     1741 gtttttcgtt gagatcgctg ttaccgctaa gcatttctgc cgagtcagaa tagctaaagt
     1801 gcgcacgacg ctgcaccact tccgtcaggg caaacgcttg ctgacgggca tcgcgctgca
     1861 tctgctgaga gtacgcgtaa tcttctttta actgttcgaa ctgttccggg tcgctctgca
     1921 ataccgaaac gatcggttcc agtttcgcca gttgattgcc aaactgctga acaaaacgcg
     1981 cggcttcctg ggcttcatcc agacgttcgc ggatttcatc gacgcgatcc gccaggctgt
     2041 catcagccaa caggttgaga cgcggcagaa tgcggttcag cgccgtaacg ccttctttcg
     2101 cctgctcaaa ctgaatacgc tgctgctggt tatcattttc atgattactc aacgcccgct
     2161 ccagttcgac tcgacggctg ttcagttgac ggatttctgc ttccgggtca gactcaaacg
     2221 ccaccgccag atgactgccg ataaagcggc tgaacgcctg atgcagacgc tgggttttct
     2281 gtacatcaaa ggagagcgtg gcgaagcgtt cggaaagcac ttcacgctcg gcatggaggc
     2341 tttcaatacg gctttcacgc gcagcacgac caaacagcgg cacttccggg aaacgtgaat
     2401 aacgccactg tcgatcggcg attttcacca ccaccgcttt ttccagctca tcaacgctga
     2461 acacgctatc atcgaatgac tgcggatccc cttcgatcaa atagagatct tccgggcaat
     2521 cggtcaagcc ttccaggtgt tcggttacct gtgacagatc tggcaccacg atggcgtggc
     2581 gtgacgggcc atacagagcc gagaagtacg gcgcatcttc caggctaacg tcgtcataaa
     2641 tttctgacag cagcacacca ccaaaacgct ccgccagcgc gttcagacgc tgatcttcag
     2701 agccgccagg ctggcttaaa cgttcgatct cttcatcgac ggcgtttttg cgcgcgccta
     2761 cttcatcgcg ttcaacaatc gcctcgcgct cacgctccag caactgttgc agatattcag
     2821 tgacgtcctg gctggaggag aactcttcgc cgcactgttc actcaactgg ttgagactgt
     2881 tttgcgctgc cagccaaacc ggtgcacgct gcatcaaact ctgaatgcga gactgcagct
     2941 gttcctgttc ctgccgcagt gccatccgct cttcacgggc gttagatacg ctatcggaaa
     3001 gagaggcaat gcgtgcttcc agctcctgat gcagagcttc cagttcatca atatcaaaat
     3061 tcttgccctg acgtttgcag aaatctgcca gcagacgctc agcttcttgc tgctcacgca
     3121 gacgctgttc cagttcgctt aagcgcatcc gcaacggctg aacctgctct gccaggtgac
     3181 gctgatcaac cccttcgcgc aatagttcgc gagcgacatc ccacgcctcg ttacgcgcca
     3241 gcgggccgtt gattgccacc accagctgat aagcctgctc aaactggctg tgcgcggttt
     3301 gcgccatgct cattttctgc tcaagagaga gcattttttc agtcgcttcc agctctttcg
     3361 cctggaaggt ttccagccat tcggcggcgc tgtcggcggt taagtccggc agatggcaca
     3421 gttctttggc acgattaagc gcagcaatcg cctggttata ctggatcgcg cgcgtttgct
     3481 ggacgtccag cgcctgctgg tagtcagcaa gctggctttt cagctcgtcc acttccagtt
     3541 cggcagcttc cgcgcgagcc tcattctctt cctggcgttc gatggcttct gccaccactt
     3601 cattttgctc ttccagacgg atctgcagct catcgagatc cacttcgtag cgttcgatct
     3661 tctcctgctg acgcaatgcg gtttgcacca ggttcaggtg atcgctggcc gcctgataat
     3721 ccgcttccag atcaccttcg gcaccgttgt gctctgccag ctcacgcgcc atatcgacgt
     3781 gtttgtactg ctcagccgcc agttgctgac gcgaagtatg tagctcgcga cgaaactcca
     3841 gggctttgtc gagatggaca cggcgctcgt tggcgtgacg catgtagtcc gccgccacgt
     3901 agttggtagc ttcgctgatc agatgcttaa acaggtcacg gtccgactgg gtgacacgaa
     3961 tcgcttccag cgtcatgcgg ttttcacgca gcgccgcttc catgtcctgg aacgctttac
     4021 gcacgccgct gttttccggc aacaggtagt cgcgcagaga acgggtaatg gcactggaga
     4081 tcccgccata cagcgaagct tcgatcagac gatagaattt gctacggtca gatgcggagc
     4141 gcagacgacg cgcgatgatg cccagatcga acatcagcga gtggtaatcg gtaatggagt
     4201 tgaactgttt aaactgcacg ccttccatcg cctcgagctt gtctttcagc tcgttaagcg
     4261 gcagcacgcg cgcctggcgt tcgttcaggg tttcggtcac cagctgtgtc ggctgcaccg
     4321 acatcggcag tccctgaatg gcaaatggtt tgatatccac tttacgatcg cgtccggcaa
     4381 cctgttgcag acgcacaccg accaccacgc gctggtggcg cgagttaatg gtgtcgagca
     4441 tcgaataaca gacacccgct ttcagcttac cgtgcagacc tttatcgcgc gaaccgctgg
     4501 tggccccggc ttccgtagtg ttacggaaat gcagcagggt caggtcgggg atcagcgccg
     4561 taacgaacgc cgccatcgtg gtggatttac ccgccccgtt accgccggaa agcgtcgtga
     4621 ccagctcgtc aaggtcaaaa gttcgggcaa aaaagccgtt ccagttaatc agcgtcagtg
     4681 agcgaaattt accgcgttca atcattattc ttcctctccg ctatctggct gactctcttc
     4741 ggtttcatcg ttgagttgca gatgattttc aatcggcatt gcttcgccat cacgaatcag
     4801 gcgacgctgt gcttcacggg gatcgtcgcc agcacgcaca tcggctccga agcggaacac
     4861 cgattcggta atgcggaact tgctgctgtc gtggcccata aaccacacca tgcctaaacg
     4921 acgcagacgg ttgagcgaag aacgtacttt ctcctgcaac ttctgacggt caacgtctga
     4981 accggttgaa cggttgttca ccagtttcag cagtttcgct tcatcggcca gggtgagcag
     5041 ttcgtcgtac agttcctgct gggtgaaaat cccctcattc gccagccgtt ccgggctgag
     5101 atagagataa cagaggattt tcccgaccat catatccagt tccgacaaga cggaacgcgg
     5161 gataagagtg gtggaacgtg ggcgtaaata gaagaaccct tccggtgcgc gaataagctc
     5221 aacgttataa cgcgcgtaaa actcttccag atattcctga aaatccatca ggaatgcatg
     5281 attatccagt tcgtcgaggc caatatggcg gcctgaacgt aaggcgctgt ccagcgccgg
     5341 aaataacgga ttcgccagcg cctgcgccag cttaaccggc atcacttgtt caatatttgt
     5401 cgatgacatg cgcctgtacc ttggctccgt aatcattaat cggctgccat ttcgctggca
     5461 gtccggtgaa atctgcttgc gctacgccaa gacgtaccgc ctgatcaata acaatacgcg
     5521 caacgtcaaa gtgacgtgca cgcggatact gtgacagata ttcgcgtacc accagaccaa
     5581 gatccagcgg cacttgtctg gttttgtaca cggcaagttg ttcttcgatg atcgccgcca
     5641 gctgttcgcg gatctcgtta aactcttcgt attccagatc ctcaggaagt tccccagtca
     5701 cttcttcatc gcgcagtgcc atctcttcgt cacgcatatc cagcagacga tcggcattgg
     5761 cataagttag cgcccacggc tcatcaaaat aggtttgtac cgactgacgt aaccgctgag
     5821 caaagacgcg gtttttatcc atatcgatcg cggtacgaat aaatttgtgt acgtggcggt
     5881 cgtagccaat ccacaagtcg atggattgct ggccccaact gataatacga tcgagtttgc
     5941 tctgcagatc gaacaccaga cgatcgacga aatgcagatc gtcatgggtc atcgtcgcat
     6001 cctgaatgcg caacagatta gcctgcaatt tgtcgcctgc cgcttccagc gtatcctgca
     6061 attcacgcag cgttccggaa gtttccgaaa gcaacaattc acagctggaa atcgccgccc
     6121 gccagtcttt gttcagcaac tgggcgatat cgtccttcac ctgctgctgc tgttcgtcca
     6181 tcagacgttg cgtcaggtcg atactgtcga aaatttctgc taccgaatat ttcagtggcg
     6241 catagacatt acggtgccag tgaaattcat caccgccctc ttcggcggca tctgctgcgc
     6301 gtttgagctc acccgccaca atcgacaact gcatagaaag acgcagcgta gaaaactcgc
     6361 gctgacggat gtagtagtca gtaatgccga tgccgagcgg cgtcagacgg taaattgcgt
     6421 tcccttccgc ctgctcgctg gtaaagcggt tcagcagacg ttgacgcacc atgtcgttga
     6481 tcgcgttatt ggcgcgcacg ccgatggttt cgctggtttg ctcaaacgca tcactcacat
     6541 ggcggaatgc atccaccagc tcgccttcac tcatctcacc atccagacgc tcgccgttca
     6601 gcgtggcaac cgccagcaga aaagagagtc ggtctaccgg cagcgagatg gagaagtcat
     6661 tttttctggc ccaggcaacc agttcgggga ctgtctggga aaattcactc atactttatc
     6721 cttgctctgc ggtttgcgcg cggtgacatg aatataacgc cctagggtaa tatacggttc
     6781 ctgacggcaa taacgcgttt ctaattcaag taatgcttca tagcagtcgc gctgctggta
     6841 cttctcgcgc agataatcat gaaacacgcg aacgcctgtc ttacccataa tttgccaacc
     6901 agcttcttcc agccacagat aaacctgcgc cgggtcgcgt ggataatctg gcgaaagcgt
     6961 ccgttttttc tttttcggca ttcccgcctg cacgtaatca aaattcccgg cgaccatgtt
     7021 atgcatcaac aaaccatgcg cattgtagaa cattaacgac aacactccgc ctggacgcaa
     7081 cactgaccac agggtctgca atacactgcg gggatcggcc acccactcga gcaccgcatg
     7141 gaacaatatc agatcaacgg gcgtttccaa atgcgaagca acatcctgag cggcgcaatg
     7201 tataaattgc atgttgtcgc tcacaccttt tgcttctgcc gcctgttttg cgcggtcgat
     7261 catctgcgca gaaagatcgc ataaaataac ctgatgcccg cgctcggcca ttttgattgc
     7321 ggtctgccct tctccgccgc cagcatccag cacacgcagt ttttgcgggc ccatttccgc
     7381 cagcacgcga tcgagatcct gccacagaat agcctgtcga agctgccctt tggtggtgcc
     7441 gtaaatgtta cgggaaaact tttccgcaat atcatcaaaa ttgcgatcct gcattgcttt
     7501 ctccaccggt tggctaagac gttttctgat aaagacgcta ttttgtcaca gccacggcga
     7561 gaatgaaccc gtctggtgta atatcgctag cgatcccttg ctatatggtt aaaaaaggaa
     7621 ccagaaagga tgctttttac actgaaaaaa gtgattggca atatgttgct gccacttccg
     7681 ctgatgttgt taattattgg cgctggcctg gcgctgctgt ggtttagccg ttttcaaaaa
     7741 accgggaaga tttttattag cattgggtgg ctggcactct tgttattgag tttgcaaccg
     7801 gtagccgacc gcctgctgcg tcccatcgaa agcacctatc cgacatggaa taattcgcaa
     7861 aaagtggact atatcgtggt gctcggaggt ggttatacct ggaacccgca gtgggcaccg
     7921 agctctaatt taatcaataa cagtctgcca cgcctgaatg agggtatccg tttatggcgc
     7981 gaaaatccgg gttcaaaact gatcttcacg ggaggcgtag caaaaaccaa tacggtgagt
     8041 acagcggaag taggtgccag agttgcgcaa tcgctgggcg taccgcgcga gcaaattatc
     8101 accctggatt tgccaaaaga taccgaagaa gaagctgcgg cagtgaaaca ggcgattggt
     8161 gacgcccctt tcctgctggt gacctccgca tcacatctgc cgcgggcgat gatctttttt
     8221 cagcaggaag ggttaaatcc gctaccagcc ccggcgaatc agttggccat cgactcgccg
     8281 cttaatccgt gggaacgggc aatcccttct ccggtctggc tgatgcatag cgatcgtgtt
     8341 ggctatgaaa cgcttggccg catctggcag tggctgaaag gtccgtcagg cgagccacgg
     8401 caagagtgat tttgatgcca gatcgaagtt gcgccgacta aatcgtccgg tattaaccag
     8461 ttgcgcaact tcatcccata acacatacaa ccaacgccgc cagatgaacg actccgccac
     8521 tggcgcgcgt tgcaggtaac tccagagcaa atcttccgcc agagaattat ccattagtcg
     8581 gaacagttcg tattctcgcg gtgcccaaag cattagtccc ggcccgacca tcgccagtaa
     8641 ctgatcgctg cgcgaatctt tcagcatgct gcgtaaacag aagttaccgt gaatcaacac
     8701 acaattgtcg ttaaagcctt caaataatgc cgggagacat tcgcgagtgc gaaacaggat
     8761 ccgcttatcc tgcatcgtca gaccggtgtt attgaactga ttgagcgtgg tccatagcac
     8821 ttcaacatgt tgccggtacc atgagggcca gaaattttcc tgagtattgt cgaccgcgcc
     8881 gacgcaaccg cgactgtcct gacggtgcca ggccagtaag gcttcaacga tttggtcttt
     8941 gagctgttcc cagcgttctg gtgttcgggc tggtgcctcc accgaaacac cacgcatccg
     9001 ctccagtagc aggacatcag ggccggggtg ttcttcatgc gtcatcacac cataaatagt
     9061 cggcatacgg acagtcccac ttcttgccag catggtggtt ttccatgcca gttgtcgggc
     9121 ttttccgggc gtactaaagc ttcttgccat taacggcatt gggtttccct ggctgtcata
     9181 caaagcccac aacgccgtat ccgctttttc attgacgcac tcaatacgac tgagtttttc
     9241 gcccagtaaa tggcttaatt cggcacgcag ctgttccatt cagattaccc tcataaaagc
     9301 tactgctttc ataatgagcg ccaatataaa agatgtcacc tgaatgagat caattgttga
     9361 aaagaaaaat tgaggaaaat cgtaacggta cgacactcct cccaaaattg gctgaagtgt
     9421 cgtgaagtga aattagcgca tttcagcgcg aacgcgctca agatcttcag gggtatccac
     9481 acctgtgcca ggaacttcct gagcaacagc aacatggatt ttttcgccgt accacagaac
     9541 acgaagctgc tctaacattt cgatgtgttc taacggactt gcctgccagt tgacgtaacg
     9601 acggataaag cctgcacggt agccataaat accaagatga cgcaggaagt tatcgccaac
     9661 ggtttcaagg ccttctgcaa aacgatcacg atcccaagga atggtggcac gtgagaagta
     9721 cagtgcatac ccttcagcgt cgagaaccac tttcaccgca ttcgggttaa acgcttcttc
     9781 cgcattgtgg attggcaccg ccagagtcgc catacccacc tgacgctgag cgaggttatc
     9841 agcaacctga cgaatgattg tcgcagggat catcggttca tcaccctgca cattaacgat
     9901 caccgtgtcg tcgctgaatg cgcatttttc gacaacttcc gccagacgtt ctgttcctga
     9961 ctgatgatcg gcgcgcgtca tacatacttc accgccagcg gcttcaacgg cgcgggcaac
    10021 atcatcatga tcggttgcca cgatgatgcg ctcggcacct gattcacgcg cgcgttcaag
    10081 aacatgaaca atcatgggtt tgccgttaat atcaaccaat ggtttaccgg gcagacgcgt
    10141 ggacgcgtag cgcgcgggaa taatgaccac aaaactcatg atttactctc atcagcagtc
    10201 agcacgcggg cttccgtttc cagcaacact ggaatgccat cgcggagcgg gaaagccagg
    10261 ttatccagtt tgcaaataag ctcttgtttt tcctggttat accaaagttt tccgttgcaa
    10321 accgggcagg caatgatttc aagcaaacga tgatccatag ttcctccaga tggatcgggt
    10381 tatgaatgca taaatcttat cataatcatt tatgcgacgg ggtctatttc ccagccagtg
    10441 cggcaatcag taaagagacc ttgcgggcag cgtcctaatg tcacccgcgt tgcctgctgc
    10501 cagctcgcga aatcagtaat cgcctgacgt aacccttttt gcagcattgt cgttggttta
    10561 atgccttcct gtaaccacag agagataact tcaaggatgc ctgtctggcg atgcattttg
    10621 gcatccattc gcccaactaa ttgcccacga tgtaataatg gcagaacaaa atagccatac
    10681 tggcgtttcg gcgctggggt atagcactcc agccggtagc taaaatcaaa aagctgctct
    10741 gcgcgtttgc gatcccagac aacaggatca aaaggcgaaa gtaccgcgct gtgcgttgca
    10801 gtgagctttc cggccagcgc acgctcgagt agcggcagca aatcagcatg cagccaaaga
    10861 ttgcccaatt tttcaacatg cacagcaatg atttgctgct gttcagccct cgcttcgcgc
    10921 cacgccgcca gtgccggacg tttcagccga tagtaatctg ccagccactg ttcgcggaat
    10981 attcccaggc tacgcgcact gttatccagc atgatgattt ctgcttctgt ttgcgaaacg
    11041 agatcgcgct catcatccca gtcaggcatg acacggtggg ttaaatcata aacgcgctgg
    11101 aagttgcgcc gttcaatcac catcaccttt ccggcagtaa ataaaccttc cagatgccgt
    11161 ttatgcggct tccattccca ccagccgctt gcacctttac gaggatgctc aaaatcggct
    11221 gaacgtaccg gccctttatc atgaatatgc tgaattaact gtgcaatttc cgcctcatgt
    11281 tcctgcatcc aggcgtcttt gtatttccag cccatttttt caggtgccag catgcggtgg
    11341 cgaataagac gaaagtcgct acgcggcata aagcaggctt catgcgccca gtattccatt
    11401 aattcgccac gcgccagaga ctcatccagc cactgggcag gataatttcc cagacgactg
    11461 aaaagcacca gatatggact acgggcaaca atattgatgg tatcgatttg cagcaaggac
    11521 atgcgggaga tcgttgccgg aatatcctcc aacgacgctc gacggcgggg tttgtttaac
    11581 aggccttgtg cggcaaggtg aagattacgc gcatcagcaa gggagaggtg cggcagcgac
    11641 attcatgact ccatcaatcg aacgctgccg cggcgtaact agttgccaga agccagcgag
    11701 gttagttgcg taagcagttt cgctggttca tcacctgaaa gctgtgcgtc tacaggcaaa
    11761 taccaccaat tttcttctgc aaaggcccgg catttcaccg catctttttc agtcattacc
    11821 agcgtttgcc cggcgcttac caacgcactg acatcctcat ggttcaaaga ctgatgatcg
    11881 gccagcggta cacatttttc cggttgtacg ccacacatct tcagcgtggc aaaaaagcgc
    11941 ggcggatgcc caatccccgc catcgccact acatgttcaa gctgagcaac gtcacaacgc
    12001 gtaccggtac gtaaattcac cgcctgaccc ggcagcagat gcatggggat ttcaccgctg
    12061 cgagggacac cgccgttgac gattaccgca tcaaccgact ttaagcgccc cgctcgctca
    12121 cgcattggcc ccgccggcaa ccaccagcca ttgccaaagc gacgcacacc atcaataacg
    12181 acaatttcca catcacgcgc cagacggtaa tgctgtaaac cgtcgtcggt tacgatgatc
    12241 tgcacatcag ggtgttgcgc cagaatggct tttaccgcat cagaacgaac gggagaaacc
    12301 gcaacaggcg catcagtgcg ttgataaatc aacacaggtt catcacccgc ctgtgctgtg
    12361 gtggtatctg ccgacaataa cagcggataa gattcagcct taccaccata tccccgcgat
    12421 acgaccccca cgcgaatacc gcgctgttgc aactgttcca ccagccagac aacgaccggg
    12481 gtttttccgt tgccgcctgc ggtgagatta ccaaccacga caaccggtac gggggcacgc
    12541 caggcgcgct tcagttttag tttatagcaa agacggatcg cgccactcac caggccatac
    12601 aaccaggaga gtggcagcaa tagccgccac aaaggggatt caccagacca gattttttcg
    12661 atcattggcc aaactgcatt ttgtgaagtt gcgcgtaaac gccgcggtgc tcaagcaaat
    12721 cgttatgcgt accgcgttcc acaatgacac catcctcgac gaccacgatt tcgtctgcct
    12781 tttcaatggt agacaagcgg tgggcaatca ccagagaggt acggtttttc tgcaactcat
    12841 ccagtgccgc ctgaatcgca cgttcggatt cggtatccag agccgaggta gcttcatcca
    12901 gaatcagaat cgggctatca cgcagcaagg ctcgagcgat agcaatacgc tgacgctgac
    12961 cgccagagag cagcacgccg ttttcaccaa tcactgtatc gagaccgtta tccatcttat
    13021 tgatgaagtc catggcgtag gccatccgcg ccgcttcttc aatttgctca cggctgtact
    13081 gttcagtccg tgcgtaagca atgttgttag caaccgtatc gttaaacaga tggacattct
    13141 gcgacaccag agcaacctgg ttacgtaacg acgccagggt atactcgcgc agatcgtgac
    13201 catccatcag gatttcgcct tcatcaatat cgtaaaaacg cgtgatcagg ctggcgatgg
    13261 ttgatttacc tgaaccagag cgtccaacca gagcaaccgt cttccctgcc ggaattttca
    13321 ggttgatgtt acgcaatgcg ggtacgtcac gtcccggata agtaaaggtg acattgcgga
    13381 attccacgtc gccagtcgca cgctcgatca cgcgcttacc ttcgtctttc tcctgctcac
    13441 tgtccagaat ggtaaacagc gtctgacaag ccgccatacc gcgctggaac tgggcgttaa
    13501 cgttggtcag cgatttcagc ggacgcatca gtgcaatcat tgaagagaaa acaacggtaa
    13561 tcgtaccggc agtcaggtta tccataacac ttgggaagct cgccgcatac agaacaaacg
    13621 ccagcgccaa agaggcgatc agctgaatga tcggatcaga gatggaagag gctgaaacca
    13681 ttttcatccc ctgaagacgc attcggttac tgactttatc gaagcgtttc gtttccactt
    13741 cctgaccacc gaaaatcaat acttctttgt ggcccttcag catttgttct gcactggtgg
    13801 tcacttgccc catggtgttc tgcatgtttt tactgatgtt gcgaaaacgc ttcgatacta
    13861 cgcgaatcgc aatcgaaaca atcggtgcca gcacaatcaa aatgatcgac agttgccaac
    13921 tgtaatagaa catcatgatg aacaggccga tgatcgacgc accttcacgc acaacagtaa
    13981 tcagtgcgcc ggaagaagaa gaagcaacct gttcggaatc gtaggtgata cgtgacaaca
    14041 gcgtacccgt tgactgtttg tcaaagaatg aaactggcat tcccatcatg tgaccaaaca
    14101 ggcggcgacg catggtcatt accacctttc ctgataccca ggagatacag tagctggaga
    14161 catagctggt gataccacgt aaaatcatca gtccgatcac caccagcggc atccacacca
    14221 gcacggagcg atctgtttta ccaaagccat catcaagaag tggcttaagg agcgataaca
    14281 tgaaggtatc gctggctgcg ttgaggatta acgctacgcc cgccacgatc agacccgctt
    14341 tgaaaggcgc aatggttggc cacagtcggc ggaatgtctg ccacgtagag agatctttgt
    14401 cgttatgcat tcaaaaaacc agcatttgtt gaaatagccg catattctac ccgttatcca
    14461 ctggcacgcc aaaccactga tgataccaac gaggtaaaat ttgctctctg aggctgctaa
    14521 tccgccagcc ttgtgctgaa aaattgaccg tcacttgccc ctgatgtgga gtatcaagcc
    14581 attgatatcc tttctgttga tagcgatgct taactttgtt agagggcaat cgccatgcgt
    14641 tatagcgcga tgccgatgcg agtgccactt ttccattcac tcgctgaatt aatggcaacg
    14701 atgatgaggt attactgcca tggtgaggta cctgaagcaa tgttgcctgc acttgctgcc
    14761 agtaacggct tagcatcttt tgttcagctg gggcttcaat atcaccggtt agaagaatgc
    14821 tattcgtccc gtcatcaacc ttaaccacac aggaatggtt atttccctta tcgttgctac
    14881 cttgtaaagg ccagtgcgcg ctgaaacgca atccttgcca ctgccacgct tcgccacgca
    14941 cacagggctg atgatgttcc cagtttaacg gacttctgat ccataacatc ggccatatat
    15001 gcaatattga atccagccct ccccggtgat ccagatgttc atggctcaga ataacgcctt
    15061 ccggttcaag attatgccag tggagccagg ggataatcag ttgttgccca ctatcccctt
    15121 caggccagtc ctgtgtcata gagaatcgct ttgccgtttc tggctatcac cattgccagc
    15181 ccttgcccga catcaagcat gtacagctgc cactcgtcag gtcgaggttt ttgccacagc
    15241 ggccagcaca tcagcaagcc tccagccaca cacattgctg gcaacgttcg ccaggcgttt
    15301 aatcgccata ccaccagtaa gaaccatggg gaaaatgata gccattgcca acattcaaca
    15361 atgttgatcc acccttccgg caaactcttt aacccccaga aaagtaaagc caaagaccgg
    15421 tcggcaagaa accataaccc ctgttccagg attaacggcc cgctaaaatg cacaaccatc
    15481 gcggcgagga tcaacggaac cgtgataaat gtcaccaagg gaattgcaaa tagatttgca
    15541 ataaacgagg tcagactaat gccatgaaat atgacgattt gcatgggcat aagcaggagt
    15601 gtgattccca gttgcagatg gatgagggaa acaactgcac gcaataccgg cggcagttgc
    15661 cactcaggac agggaaacca ctgataccaa aaaatcagtg ccgcgaccgc agcggcagag
    15721 agccataaac tttgcgagag aatggcaaca ggatccatca gcaaaattgc cgccagacaa
    15781 catatccata catcccagcc actccactgt cgcccactta acttaagcat tccccacgta
    15841 gcaagcgcca ccacggtacg caatgcagga ggttgcattc ccgtcaacca ggcataaaaa
    15901 gcagcacagc agattccgcc aattaatggt atttgccagt ggatccagcg cccaggcaga
    15961 aaaatttgtc cactgcgaat gagtccggca gccagcaacg ccgcaaaagc gatgtgcaat
    16021 cccgatatcg ccattaaatg cgccgttcca gtatcgcgca tgatattttt gatttctttg
    16081 gggacggata accgttcccc catacctaaa ccaagaataa ccgcattcca cggatagggt
    16141 tgcagcgttg tttgtagtga cgccagatac tgtgcacgca ggctacaatt cggttcaatg
    16201 acacttgcct gcagaaaacg gccggtgagc ggctgatgct gggcaatggc ataacgctgg
    16261 ctatcaaagc cgccatcatt aagttggcca tgaactgcac gaactttgag tttcattgac
    16321 cattgttgtc cagcacaaac cgcttgcgga agttattcgc catacagcac aaggcctggc
    16381 gcagggaata tacgtcgacc ttgtagataa gtaatttgac cgtaatgagt ggtcatgccg
    16441 tcagttgcag tgatctcaac aattgcatcc tgcgtcgcgc cagttaaggt ttctcctgcc
    16501 cacaaaattt gctttgctga taatatgccc caaacgaaaa acagcaacgt cagcgcgata
    16561 taacggacgg ttttaacagg gataaatgcc agtacgcagg cgaagagagt cagaaacgca
    16621 agggttaatg tcctaggcaa ttggggcaaa atcagcaacg gaaaaattcc gcaaattatg
    16681 catacgccga ctgtcgttat tttcatgcac ccctccctgt ttatggaaag tgtgcaactt
    16741 tgttgagcgg ttgtctggtg ggaattttgc gttccccgag gcacattcag aagaatttaa
    16801 cgaaagggtg aaaactgctc aaaactctgc gaggcagctt ccagttcctg attagagaat
    16861 tgaaaaaagc acccgacagg tgcttttctc gcgttcaagt ttgagtaaaa acttaaccgt
    16921 aaatattggc gcgatcgcgc agttctttac caggtttaaa gtgaggaacg tattttcctt
    16981 ccagttctac tttatcgcca gtcttcggat tacgtccggt acgtggtgcg cggtagtgca
    17041 aagagaaact gccgaaaccg cggatttcaa tacgctcgcc ctgcgcaaga gtcgaggcca
    17101 tatgctccag catctctttt actgcatctt caaccgtctt ggcgggaatg tgcgattgct
    17161 gggtggcaag tctttctatc aattctgact tggtcatgat tcctccggtt ccttaaaggc
    17221 aaattaagcg gctgctgttg ctgcattgat taagggcggc cgtagccgcc cttagtgctt
    17281 gattacagga cgaaacctgc aatctgtcaa gtaaactcaa caaacttcgg aataaaaatc
    17341 ccgaagagtc agagaattac tcgcctttag ctgctttgaa agcttcagcc attgcgttgt
    17401 tggagaagtt tgcatcttcc tgtttgttaa cagttgcgat tgcatctttc tcgtcagctt
    17461 cgtctttcgc acgaacagac aggctgattg cgcggttttt acgatcaacg ccggtgaatt
    17521 tagcttcaac ttcgtcgcca acgctcagaa ccagggtagc gtcttcaacg cggtcacggg
    17581 atgcttcaga agcacgcagg taaccttcaa cgccgtcagc cagttctacg gttgcgcctt
    17641 tagcgtcaac tgcagttact ttaccggtta cgatagcgcc tttcttgttc agagcaaccc
    17701 agttgttgaa cggatcttct gcgagctgtt taacgcccag ggagatacgt tcacgttctg
    17761 cgtcaacctg cagaacaact gcagcgattt cgtcgccttt tttgtattca cgaactgctt
    17821 cttcgcctgc aacgttccag gagatgtcag acaggtgaac caggccgtcg atgccgccgt
    17881 ccaggccgat gaagataccg aagtcagtga tagacttgat tttaccttca acacggtcgc
    17941 ccttgttgtg ggtttccgcg aactgctgcc acgggttagc tttgcactgt ttcagaccca
    18001 gggagatacg acgacgttct tcgtcgatat ccagaaccat aacttccact acatcgccaa
    18061 cgttaacaac tttggacggg tggatgtttt tgttggtcca atccatttcg gaaacgtgta
    18121 ccagaccttc aacgccttct tcgatttcaa cgaagcagcc gtagtcagtc aggttggtca
    18181 cgcgaccagt cagtttggta ccttccggat aacgtttagc gatagctacc cacggatctt
    18241 cgcccagctg tttcaggccc agggatacac gggtacgttc gcggtcgaac ttcagcactt
    18301 taacagtgat ttcgtcgccc acgttgacga tttcgctcgg atgcttaacg cgtttccagg
    18361 ccatgtcagt gatgtgcagc aggccgtcaa cgccgcccag atcaacgaat gcaccgtagt
    18421 cagtgaggtt cttaacgata cctttaactt ccatgccttc ctgcaggttt tccagcagct
    18481 gatcgcgctc tgcgctgttt tcggattcga taacggcacg acgagaaaca acaacgttgt
    18541 tgcgcttctg atccagcttg attactttaa attcaagctc tttgccttcc aggtgcagag
    18601 tgtcacgcac cggacgaacg tctaccagag aacctggcag gaacgcacga ataccgttca
    18661 gctcaacagt gaagccgccc ttaactttgc cgttgataac accggtaaca gtttcagcat
    18721 cttcgtaagc tttttccagc gtgatccagg cttcgtgacg tttagctttc tcacgggaca
    18781 gcagagtttc accgaagccg tcttctactg cgtccagagc aacgtcaact tcgtcaccta
    18841 cctggatttc cagctcgccc tgggcgtttt tgaactgctc agccgggatg gcggactcag
    18901 atttcagacc agcgtcaacc agtactacgt ctttgtcgat agcaacaaca acgccacgaa
    18961 cgatagaacc cgggcgggtt tcgatttctt ttaaggactc ttcaaagagt tgagcaaaag
    19021 attcagtcat gtttaatctt caggtttata tttaacgtcc acctggctcc atgccggatg
    19081 gggttgttta acatacccgc tgtcattcca ttgcaacggg ggtactgcaa attcggtcgc
    19141 ttatgcgaga gccaatttct ggcgcgcgta ttgtagcgct ttttcaatca attgctcaat
    19201 gcttaaggtg gtggaatcca acactaaagc atcggctgcc ggaaccagtg gcgctaccgc
    19261 tcggttacga tcgcggtcgt cgcgttcttt gatctcggcc aaaaggcgct cgaagttaac
    19321 actaaagccc ttctcctgca actgtagcat gcggcgatgc gcacgttctt ccgaggaggc
    19381 gtcaaggaaa attttcactg gtgcatcagg gaataccacc gttcccatgt cgcggccatc
    19441 ggcaatcaga cctggtaatt cgcgaaacgc gcgttggcga cgcaataatg cttcacgaac
    19501 gcgtgggaat gccgcgactt gtgaagccgc attcgccact tcctgagtac gaatttcgcc
    19561 gctgacatct tccccttcga ggatcacttc cagattgcca ttggtcgaca caaaacgtac
    19621 atccagatgg gatgccagcg gtaccagcgc atcttccgac gcaacatcaa catggtgatg
    19681 taatgccgcc aatgccagta cgcgataaat tgcacccgag tccagcagat gccattgcaa
    19741 cgcttccgcc atagccttac acaaggtgcc tttccctgca ccgcttgggc catcaatggt
    19801 aataaccggg gcaattgccg tcatctttat ctccttaaaa caggcgtacc gttaacataa
    19861 ccgcgcgcat tatacgcgcc gacgccacaa atcgttaacg ttgtgtgcaa aaaccagttt
    19921 tgtctgtatt cagatgggga tttattgcgc aattatagcg ggctggcaga agaccagccc
    19981 gaaagatgac aatgatttac ttaccagagg cgatacgatc acggatgtgt tgcgcacgct
    20041 ctgttgatgg tgggtgagag tcaaacatgg actgggtgcg accgccatcc aggctagcca
    20101 gtttttcaaa gctgccaacc agcccctggg tgctgatgcc acgtttcttc aacagatcaa
    20161 aagagaaatc atctgcttcg gactctttat cacgggagta tttagcatta atagcgcctt
    20221 ctgcgatatc acccaattgt gagcgggaaa gctgggaagc cacaccgctg gtagctgaaa
    20281 tggcatcgcg tgcggcaacg atcgcataag aagctttcat ttcagccagc gagtgaccca
    20341 acgcgacgtg gcccagttca tggcccagaa caccttcaat ttcgttgtca ttcatcatgt
    20401 ccatcaggcc actgtagaca cgaacacagc cgttcgccat cgcccatgcg ttgacgtcgc
    20461 tggtcatata aaccttatag ttgaccggcg tgccattaat attgttaccc aatgctttgg
    20521 cgatttttgc cagacgttta ccgtacttgc tggatttact tgcgacttga ttgccgctgt
    20581 ccatttcggc acagccctga ttggcaatcg cttttgcatc ggcatcgctt aatgttgctg
    20641 ctttataggc atttaaaccg gagctgatag ccatattggt atcaataccg tgggtatttt
    20701 gacaccctgt cagtaatgct gcagaggtcg caatcgccag cagtaattta gtattcttca
    20761 tgtgaatcat ccgttgatta atcatatata aatattatgt atcaacaact tacattgaca
    20821 agcgacgcta ccattaatca aaatgactca caaggtccga aaaaaaacgc cgacaat
//
LOCUS       DAZPDE010000058        20780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPDE010000058 DAZPDE010000000
VERSION     DAZPDE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.058"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHU0J_001957"
     CDS             <1..579
                     /locus_tag="ACHU0J_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1216661.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHU0J_001958"
     CDS             597..1235
                     /locus_tag="ACHU0J_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1216662.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHU0J_001959"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHU0J_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1216663.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHU0J_001960"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHU0J_001960"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1216664.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHU0J_001961"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHU0J_001961"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1216665.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHU0J_001962"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHU0J_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1216666.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHU0J_001963"
     CDS             complement(5552..6613)
                     /locus_tag="ACHU0J_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1216667.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHU0J_001964"
     CDS             complement(6610..8007)
                     /locus_tag="ACHU0J_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1216668.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHU0J_001965"
     CDS             8163..9161
                     /locus_tag="ACHU0J_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1216669.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHU0J_001966"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHU0J_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHU0J_001967"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHU0J_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHU0J_001968"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHU0J_001968"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1216670.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHU0J_001969"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHU0J_001969"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1216671.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHU0J_001970"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHU0J_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHU0J_001971"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHU0J_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHU0J_001972"
     CDS             complement(16089..17141)
                     /locus_tag="ACHU0J_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1216672.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHU0J_001973"
     CDS             complement(17172..18014)
                     /locus_tag="ACHU0J_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1216673.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHU0J_001974"
     CDS             complement(18001..18882)
                     /locus_tag="ACHU0J_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1216674.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHU0J_001975"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHU0J_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHU0J_001976"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACHU0J_001976"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR1216675.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5669 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttgccctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAZPDE010000059        20691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPDE010000059 DAZPDE010000000
VERSION     DAZPDE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.059"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            81..350
                     /locus_tag="ACHU0J_001977"
     CDS             81..350
                     /locus_tag="ACHU0J_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1216676.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHU0J_001978"
     CDS             344..709
                     /locus_tag="ACHU0J_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1216677.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHU0J_001979"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHU0J_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1216678.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHU0J_001980"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHU0J_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1216679.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHU0J_001981"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHU0J_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1216680.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHU0J_001982"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHU0J_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1216681.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHU0J_001983"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHU0J_001983"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1216682.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHU0J_001984"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHU0J_001984"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1216683.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHU0J_001985"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHU0J_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHU0J_001986"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHU0J_001986"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1216684.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHU0J_001987"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHU0J_001987"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1216685.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHU0J_001988"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHU0J_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR1216686.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHU0J_001989"
     CDS             complement(16429..17187)
                     /locus_tag="ACHU0J_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1216687.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHU0J_001990"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHU0J_001990"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1216688.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHU0J_001991"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHU0J_001991"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1216689.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHU0J_001992"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHU0J_001992"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1216690.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHU0J_001993"
     CDS             complement(20306..20587)
                     /locus_tag="ACHU0J_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216691.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4888 a         5692 c         5217 g         4894 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggtt acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg ccctgatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZPDE010000060        20523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPDE010000060 DAZPDE010000000
VERSION     DAZPDE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.060"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACHU0J_001994"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACHU0J_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1216692.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACHU0J_001995"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACHU0J_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR1216693.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACHU0J_001996"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACHU0J_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1216694.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHU0J_001997"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACHU0J_001997"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1216695.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHU0J_001998"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACHU0J_001998"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1216696.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="ACHU0J_001999"
     CDS             4451..4816
                     /locus_tag="ACHU0J_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1216697.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="ACHU0J_002000"
     CDS             4966..5484
                     /locus_tag="ACHU0J_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1216698.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHU0J_002001"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="ACHU0J_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="ACHU0J_002002"
     CDS             6715..7197
                     /locus_tag="ACHU0J_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1216699.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHU0J_002003"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACHU0J_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1216700.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHU0J_002004"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACHU0J_002004"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1216701.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHU0J_002005"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACHU0J_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1216702.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHU0J_002006"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACHU0J_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1216703.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHU0J_002007"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACHU0J_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1216704.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="ACHU0J_002008"
     CDS             10941..11732
                     /locus_tag="ACHU0J_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1216705.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="ACHU0J_002009"
     CDS             11753..13039
                     /locus_tag="ACHU0J_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1216706.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHU0J_002010"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACHU0J_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1216707.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="ACHU0J_002011"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="ACHU0J_002011"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1216708.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="ACHU0J_002012"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="ACHU0J_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1216709.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="ACHU0J_002013"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="ACHU0J_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1216710.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="ACHU0J_002014"
     CDS             complement(16987..17277)
                     /locus_tag="ACHU0J_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1216711.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHU0J_002015"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACHU0J_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1216712.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="ACHU0J_002016"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="ACHU0J_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1216713.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHU0J_002017"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="ACHU0J_002017"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="ACHU0J_002018"
     CDS             19139..20380
                     /locus_tag="ACHU0J_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1216714.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5359 c         4815 g         5049 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAZPDE010000061        20411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPDE010000061 DAZPDE010000000
VERSION     DAZPDE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.061"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(20..1669)
                     /gene="actP"
                     /locus_tag="ACHU0J_002019"
     CDS             complement(20..1669)
                     /gene="actP"
                     /locus_tag="ACHU0J_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1216715.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1666..1980)
                     /locus_tag="ACHU0J_002020"
     CDS             complement(1666..1980)
                     /locus_tag="ACHU0J_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR1216716.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2180..4138)
                     /gene="acs"
                     /locus_tag="ACHU0J_002021"
                     /pseudo
     CDS             complement(2180..4138)
                     /gene="acs"
                     /locus_tag="ACHU0J_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4531..5967
                     /gene="nrfA"
                     /locus_tag="ACHU0J_002022"
     CDS             4531..5967
                     /gene="nrfA"
                     /locus_tag="ACHU0J_002022"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1216717.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6012..6578
                     /gene="nrfB"
                     /locus_tag="ACHU0J_002023"
     CDS             6012..6578
                     /gene="nrfB"
                     /locus_tag="ACHU0J_002023"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1216718.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6575..7246
                     /gene="nrfC"
                     /locus_tag="ACHU0J_002024"
     CDS             6575..7246
                     /gene="nrfC"
                     /locus_tag="ACHU0J_002024"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1216719.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7243..8199
                     /gene="nrfD"
                     /locus_tag="ACHU0J_002025"
     CDS             7243..8199
                     /gene="nrfD"
                     /locus_tag="ACHU0J_002025"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1216720.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8219..9937
                     /locus_tag="ACHU0J_002026"
     CDS             8219..9937
                     /locus_tag="ACHU0J_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1216721.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9930..10313
                     /gene="nrfF"
                     /locus_tag="ACHU0J_002027"
     CDS             9930..10313
                     /gene="nrfF"
                     /locus_tag="ACHU0J_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1216722.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10310..10906
                     /gene="nrfG"
                     /locus_tag="ACHU0J_002028"
     CDS             10310..10906
                     /gene="nrfG"
                     /locus_tag="ACHU0J_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1216723.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11248..12561
                     /gene="gltP"
                     /locus_tag="ACHU0J_002029"
     CDS             11248..12561
                     /gene="gltP"
                     /locus_tag="ACHU0J_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1216724.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12639..13328)
                     /gene="yjcO"
                     /locus_tag="ACHU0J_002030"
     CDS             complement(12639..13328)
                     /gene="yjcO"
                     /locus_tag="ACHU0J_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1216725.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13422..15569)
                     /gene="fdhF"
                     /locus_tag="ACHU0J_002031"
                     /pseudo
     CDS             complement(13422..15569)
                     /gene="fdhF"
                     /locus_tag="ACHU0J_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15767..17233)
                     /gene="mdtP"
                     /locus_tag="ACHU0J_002032"
     CDS             complement(15767..17233)
                     /gene="mdtP"
                     /locus_tag="ACHU0J_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR1216726.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17230..19272)
                     /gene="mdtO"
                     /locus_tag="ACHU0J_002033"
     CDS             complement(17230..19272)
                     /gene="mdtO"
                     /locus_tag="ACHU0J_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1216727.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19272..20303)
                     /gene="mdtN"
                     /locus_tag="ACHU0J_002034"
     CDS             complement(19272..20303)
                     /gene="mdtN"
                     /locus_tag="ACHU0J_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1216728.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20322..>20411)
                     /locus_tag="ACHU0J_002035"
     CDS             complement(20322..>20411)
                     /locus_tag="ACHU0J_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216729.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4556 a         5422 c         5609 g         4824 t
ORIGIN      
        1 gaccggggaa ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg
       61 agcggataaa ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg
      121 ttgccgagaa gaaccagatg ccgaggaatg ccacgctgat agagaacagc gccgggtatt
      181 catacgggaa gatggctttt tcgtgaccga ggatctgtac ccaaatagtc gggccgagga
      241 tcatcagcac cactgcggta atcagcccca gccagccgcc cagcatcgcg ccacgggtag
      301 tcagtttcga ccagtacatg gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg
      361 caaacgcaag gcctaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa
      421 tcgcaatcac gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg
      481 tcgcgccttt tttgaagacg ttagcgtaca agtcatgcga aaccgccgat gcgcccgcca
      541 gcgtcagatc cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga
      601 ggaataggtt gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa
      661 tcagatggcc cgccgcgtct ttatattccg gattcgcacc aacaagcatg atcgcgccga
      721 agccgataat aaaggtcaga atatagaagt agcccataaa tccggtggcg tagaacacgc
      781 tcttacgtgc ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc
      841 ccgccgtacc aaacatcagt cccagcccca gagagagcgc ggagatcgga tctttcacca
      901 gtccgcccgg tttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcgctgaaca
      961 gattgttgaa gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga
     1021 acagcaacag cacagctttg ataatttgta cccaggtggt cgccagcatg ccgccgaaca
     1081 ggacgtacat catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa
     1141 acagcagctc gatcagttta cctgcgccta ccatctgagc gataaggtaa agcgccacca
     1201 ctaccagaga accacaggcc gaaagaatac gaatcggccc ttgtttcaga cgataagagg
     1261 ccacatcggc agaggtgtag cgtcccaggt tacgcagacg ttcggcaatc agaaacagaa
     1321 tgattggcca gcccaccagg aagcccaacg agtagatcag cccgtcatag ccggaggtaa
     1381 acaccagcgc ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca
     1441 gcccgttctg gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta
     1501 cgcgttttga tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa
     1561 taatcgcctg ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt
     1621 tagctgcgaa agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac
     1681 ctcatgcagg acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat
     1741 accggtgaga acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac
     1801 gctggtgttc ggattcagcg gcgtgcccag ccagccgggc gcgaaggcga tcagtaaaat
     1861 aaagccgata taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt
     1921 ttcgactaac tccctgaaat gcgcattgtc ttctatccgc tgataaatcg taccattcat
     1981 cacagattct ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag
     2041 gttaacgctc gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg
     2101 gcaatcaatg cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgatt
     2161 cgcatccggc aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct
     2221 tctcgaccac gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg
     2281 ccgcaatttt gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt
     2341 cggtccagtg cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc
     2401 ggacttctgc gtacagttct ggtgacggtt cctccccgtg attaagcgtg acataggcgt
     2461 agatcgcctg acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg
     2521 gatgcgccac cagcgccgac tctatctctg ccgtccccag acggtgaccg gagacgttca
     2581 gcacgtcgtc cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt
     2641 cgccgctgaa atacatattt ttgaaggtgg aaaaataggt ctgctcaaaa cgttcgtgat
     2701 cgccaaacag cgtacgcgcc tgaccaggcc aggagtcggt gatcaccagg ctaccttcgg
     2761 tagccccttc cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg
     2821 gacgtgttgc cgaaccggct ttcagctcgg tagcgccagg cagcggggtg atcatgaaac
     2881 cgccggtttc ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt
     2941 tccagtacca ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgta
     3001 gcgacgaacg gtcggtgcct tcaatcgctt tatcgccttc cgccatcagc gcgcggatcg
     3061 ccgtgggtgc ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg
     3121 caggcgtcgg ccagttcggt acgccttcaa acatcagcgt ggttgcgccg caggccagcg
     3181 ggccgtacag caaataactg tgtccggtaa cccagcccac atcggcggtg caccagtaga
     3241 tatcacccgg atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac
     3301 cgccggtagt gtgcagtacg ccttttggtt ttccggtaga accggaggta tagagaataa
     3361 acagcggatc ttcggcgttc atctcttccg cctagtgctg atcgctggct tgctcaacca
     3421 ggtcgtgcca ccacaggtcg cgcccttcct gccagtcgat gtttccgcca gtacgcttca
     3481 gtaccaccac atgctctacg ctggtgacgt tcgggttttt tagtgcgtca tcaacgtttt
     3541 tcttcagcgg aatactacgc ccggcgcgca cgccttcgtc ggaagtgatc accagtcgtg
     3601 agttggaatc aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg
     3661 aatgcatcgc gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg
     3721 gcatataaat cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga
     3781 agcggcagac gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt
     3841 cgccttccca gatgatggcg gtgcgatcgc cgttttcttg cagatggcgg tcaaggcagt
     3901 ttgccgccag attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa
     3961 aggaggtatt tttcaccttc tggtacggtt tgatccagtc gagaattttt ccctgttcgc
     4021 cccagaaggt atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt
     4081 ttatcaggca acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc
     4141 ttttgttctc cttgtaggat gttaataata tgtggcataa gcgttaaatg taggggtatt
     4201 ggcagttttg tttagtattt gggcgacaga tcacgcaaaa gtagaatggt gcaaataaac
     4261 ggcagggtaa tttttgaagg tcaccaacaa aagttgatta attctttgag gaacatgcag
     4321 ttatgcatgc tgttgaaaaa gaggaagata ctgactaact ctaaagtggt atttcacatg
     4381 cacttacaat tgattaaaga caacattcac agtgtggtta tttgttacac ataggggtga
     4441 gcaatgtcat gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg
     4501 caacaatggc gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt
     4561 atcttcagct tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca
     4621 gctcccgcaa aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatcac
     4681 gatcaatatc tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg
     4741 gaagatccac ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag
     4801 cctcgtggac atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg
     4861 aaaaacgctg aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg
     4921 gcgcgtctga tccagaaaga cggcgaagat ggctacttcc acggtaaatg ggcgcgcggc
     4981 ggcccggaaa tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag
     5041 ttcgccaaag gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa
     5101 gccattggta aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt
     5161 cagtgccatg tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat
     5221 gacggcatga aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg
     5281 actaactccc tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg
     5341 acagcgggca ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg
     5401 cagaacgccg aaggcaaact ctacaccgac cataaaattg gtaatccgtt tgataacttc
     5461 gcccagactt gtgcgaactg ccatacccag gataaagctg ccttgcaaaa agtggtcgcg
     5521 gaacgtaagc aatcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct
     5581 cacttcgaag cgaaagcggc gctggatgca ggcgcgacgg aagccgaaat gaagccgatt
     5641 caggacgata tccgtcatgc ccagtggcgt tgggatctgg cgattgcttc ccacggtatt
     5701 catatgcacg caccggaaga aggtctacgg atgctcggta cggcgatgga taaagcggcg
     5761 gatgcgcgca ccaaactggc gcgcctgctg gcgaccaaag gtatcaccca tgaaatccag
     5821 atcccggata tctcgaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa
     5881 atcaaggccg agaagcagga cttcatcaaa acggtgatcc cgcagtggga agagcaggca
     5941 cgtaaaaacg gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca
     6001 atggagtgaa tatgagcgta ttacgttcgt tattaactgc tggggtgctg gcgtcgggcc
     6061 tgttgtggag cctgaacgga attaccgcta cccctgcggt gcaggcatct ggtgatcgct
     6121 acgaagttac ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata
     6181 ccgaaggtat gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ttgccggtca
     6241 cctgcaccaa ctgtcacggc caagcatcac cgcaacaccg cgaaggggtg aaagatgtga
     6301 tgcgctttaa cgagccgatg tataaggtcg gagagcagaa cagcgtctgt atgtcctgtc
     6361 atctgccgga acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg
     6421 cgtgcgccag ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca
     6481 aaggacggat taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta
     6541 acccggcgtc tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct
     6601 caccggcgtc ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac
     6661 gttgaatatc aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg
     6721 taccgcctgt atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct
     6781 gacgattatt cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg
     6841 taagtcttgc cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc
     6901 ttttcgtgat gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca
     6961 gtactgcatc gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc
     7021 ggacaaatgc gatttctgtc gtaagaccaa tttgcaggcc ggtaagcttc ccgcgtgcgt
     7081 tgaagcttgc ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat
     7141 ttcgcaactg ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc
     7201 gaagctgtac cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca
     7261 tttcattttg aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt
     7321 atttctgccg gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc
     7381 ggtgcagaca gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc
     7441 ggtctgttga tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc
     7501 cactacagtt ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg
     7561 gtgctggtgc tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc
     7621 tggttgccga agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc
     7681 ggactggaaa cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg
     7741 ctgtcggcgc tgaaatcgta tccgttcctc aataacccga gcctgccggt gctgctcctc
     7801 ttctccggca tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc
     7861 agtaacccgc attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg
     7921 ggtgaaatct tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa
     7981 gttcgtgcgc tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt
     8041 gtcgccgggc tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc
     8101 ggcattcctg ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc
     8161 tttttcattc tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct
     8221 ggacgtattc cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt
     8281 gttgaccccg ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact
     8341 cacttgcatc ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac
     8401 gtattgtttt ctcatcagcg atttctcggt catttatgtc gcccaacata gctacagcct
     8461 gctgtcgtgg gaactcaaac tggcggcggt gtggggcggt catgaaggtt cgctgctgct
     8521 ttgggtgctg ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaag
     8581 cgatccgcta tttccgctga cgctagccgt tttatctctc atgctcgccg cactgctact
     8641 gtttgtggtg ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg
     8701 cgatctcaat ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct
     8761 tggctatggc ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga
     8821 gtttgatgcg gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt
     8881 aacggcgggg atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg
     8941 gtggttctgg gatccggtgg aaaacgcctc tttattaccc tggctttctg ccactgcgct
     9001 gctgcacagt ttgtccctga cacgccagcg ggggattttc cgccactggt cgctgttgct
     9061 ggcgatagtt actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct
     9121 ggtttcggtt catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt
     9181 tgcactgatt agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc
     9241 ggcggtgcgt ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt
     9301 ttgcgcagtg ctactgatcg tgctggtggg aacgctttat ccgatgattt acggcctgct
     9361 gggctgggga cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg
     9421 tctgttgatg ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgtgcagct
     9481 tccggcgctg gtagctcatg ctggcgtgct gttatttgcc gcggggatcg tggtttccag
     9541 tgtcagtcgt caggagaaca gcctgaattt acagccgggt cagcaggtga cgctggcagg
     9601 atacaccttc cgttttgagc gactcgatct gcaagccaga ggcaattaca ccagcgaaaa
     9661 agcgatagtg gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg
     9721 tttttatgaa gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca
     9781 tgactggtat gcggtcatgg gtgaaaaaac cagagcggat cgttacgctt ttcgtttgta
     9841 tgtacaaagc ggtgtgcgct ggatctgggg gggaggattg ttgatgattg cgggcgcatt
     9901 gttaagcgga tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt
     9961 tatttacctg ttttgcgcgc gctcaggttg tagacacctg gcaattcgcc aatccgcaac
    10021 aacagcaaca ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa
    10081 acttactcga atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg
    10141 tgtcggaggg gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt
    10201 ttgttcgcta taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg
    10261 tgttgttatt gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc
    10321 tcaaataccg gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg
    10381 cagttatctg ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga
    10441 tccgctacat cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga
    10501 taaaatccgc gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct
    10561 gtggcaaaac gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg
    10621 tgagaacgct gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca
    10681 tatgaccgcc cagactcgcg caatgatcga caaagccctc gcgctggact ctaacgaaat
    10741 caccgctctg atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat
    10801 cgaactatgg caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt
    10861 cgagtcgatt aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga
    10921 agattatgtc attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac
    10981 caatcagtat tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat
    11041 taatcctgcc ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa
    11101 gggttgcgca acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac
    11161 caacgcaaca caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa
    11221 cggcgctcca ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag
    11281 attctgtttg ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac
    11341 agccgcgact ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg
    11401 atcaaaatga ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt
    11461 ggtgatgcca aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc
    11521 accaccgtcg ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg
    11581 gtggatatgt cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg
    11641 gtacaaagca gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt
    11701 gtggcgtcga tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt
    11761 ctggggcttt cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc
    11821 atctctgaaa ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg
    11881 tttgcgctga ttgcggtgac ggtggctaac tttggtttct cgtctctgtg gccactggcg
    11941 aaactggtgc tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt
    12001 gtggcacgtc tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg
    12061 attctggcgt actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg
    12121 gaagcctacg gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt
    12181 aaccttgatg gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat
    12241 ggcattgacc tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg
    12301 aaagggattg ctggcgtggc tggcgtgtcg tttgtggtgt tgctggcaac gctgggtagc
    12361 gtaggtattc cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg
    12421 gcgcgtactg cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg
    12481 gaacacaaat ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt
    12541 gataaaactg cggatcaata attgaagatt gccggggata tcccccggca atgtgtggat
    12601 gcctgatgcg acgcttgccg cgtcttatca ggcctacctt aaccgttggt taatttttca
    12661 aactcttcac acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc
    12721 ttttgtttgt tcttctcgat aaaaccttcc tcaccgttta agaacatcat tcccgcccag
    12781 tactcggaat aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg
    12841 tcatcgtcgg ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca
    12901 tcgacggcgg agtcattctc cagatcttcc gaggcgtttt ctaacagcga aatcgccttt
    12961 ggataatccg gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct
    13021 tctttactgc ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga
    13081 ctgaccggat tggtgatttt gatctgcgcc aacagcgcgc aggcatcagc atcaccgctg
    13141 tcggcggctt tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg
    13201 tcggcaagaa aatattgtgc acgtcggtcc ccggcctctg ctgcctttaa atactggctg
    13261 ccaggctcgg agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt
    13321 tttttcattt tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat
    13381 aaaaaaacag cctccgaaag gaggctgtag aaaggactgt attacgccag tgccgcttcg
    13441 cgcaggcgag ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga
    13501 tcggagatcg gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt cgtaatcggg
    13561 cttaagcttt cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa
    13621 atcgcccctt tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc
    13681 gagtgcactc aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg
    13741 atttgtgcgt agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc
    13801 gaacggcaag agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg
    13861 gtgagtttgt cgattggcgc tacccagtcg caggtgaaga actgcgccag accgttcggg
    13921 gtatcaaact tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg
    13981 caaggccact gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc
    14041 gggcacagat gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa
    14101 cccatacggg tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt
    14161 tcaactgctt tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc
    14221 tcgccccacg acgttgacgg caaaatgaca tccgccgccg acgcggtttt ggttataaag
    14281 atgtcctgaa cgatgaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc
    14341 gcatcagttt gcagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct
    14401 gcgcggtgcg gcagctcgct gatgcgatag ccggtatgag caggcaggct ttccacgccc
    14461 caggctttgg cgaatttttc gcggttagcc ggatctttca cgtactggta tcccggatac
    14521 gtatccggca gcgcgcccat atcgcaggca ccctgcacgt tgttctgacc acgaaccggg
    14581 tttacacccg catgcggctt accgaggtta ctggtcagca tcgcgaggct ggtcagagaa
    14641 cgcacggttt ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg
    14701 cttttcgcct gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacaccggtg
    14761 atatcttcaa ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct
    14821 tctgtacggg aagcgacgaa cgctttgtcg tacagatttt cttcaataat gacatggccc
    14881 atcgcattca acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg
    14941 cgcgcggttt caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg
    15001 ttaattacgt gattcgccac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac
    15061 actaaatcgg tattatcaat ttcgttaata gcattgctca ttgcgccatt accgaccgat
    15121 tggtgcagac ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg
    15181 gtaccaataa cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca
    15241 cgcgaggagc cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg
    15301 cgctcggcaa cgtaattcag tgcctcatct caggaaacag gttcgagttt gccgccacgc
    15361 tggcgacgga tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaaa
    15421 tcccagccat aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc
    15481 tccgcccgga cgattttgcc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa
    15541 taggggcaaa ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat
    15601 acgcgctctc gactacagta tgcatctttt atgccacatt ttatttgggg tcattccctg
    15661 atattacggg cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac
    15721 gtgacgaaac gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga
    15781 cgacgggagc cgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca
    15841 cccggcggct gtccagcatc agtaatgaca tctcttcggc aagtactggc aaccgggctt
    15901 cagtggcctg taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg
    15961 tgaagcgcgt tgcttccacg tgttcagcct gcatttctcg ctcgtcgttg agcgtttgca
    16021 gacgcgtgcc gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt
    16081 caatcatcat gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt
    16141 caaacagcgg caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata
    16201 aggtatccag gtggatggca tcaaggccga aaaacgcttt gatatcaaag ctcggataga
    16261 acaacgcccg cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt
    16321 gcagatccgg gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga
    16381 ctcgtggtaa tgccacaggt ttgatctccg gcatatcgct ggctcccgcg ccaatcaatg
    16441 cgcgcagaga ttctctcgtt tcggtgattt gccctttgac ggcagcaatt tgtttatcga
    16501 ccgccagaat ctgtgcccgc gcgccgtgga aaggcacttg tgcttccaga ccgtgcgcca
    16561 ctttactttg gtgcgctttc accgcgtaat caatcacatc gcgagtttgt tctaacagat
    16621 cgagcatctg atagctggcc tgcatactgt aataaagctg cgctacgccc gtggtcagcg
    16681 atagctctac tgctgcggtt tctgccagcg cggcattatg cgcgccaatg gcggcggcaa
    16741 ccgctgagcg atgcacaccc cacaaatcaa gatccagtcc ggcaaacaaa cctactgtgg
    16801 cttccgtata gtacggcccg tccatcccca gtgcgggcgc atccatcgca taagggctta
    16861 aaaagccgtt cgccgagaca cgttggcggt taagcatccc taacgctgcc acctgcaact
    16921 gtgaaccggc atctaacaaa tcggcctgcg attgcgcttt ttcttcccgc agtttcgctt
    16981 cggcgagggt gtgtgaacca cttagcgtcc gttggatcag ggcatccaac tgcgggtcat
    17041 tgagttgttt ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcatcgg
    17101 ccagtttgat ttgttccggt ttgagctgtt gatgaggcgc agaatcctta cgtaccaggg
    17161 cacagccgga aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt
    17221 gacgattgat cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc
    17281 tgtccagtct ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt
    17341 gggcaggatg gcctgcgagg tgggtgtctc ttcaagcgtt atttgaggag aacggctgag
    17401 tgcggttgcc agaccggcag cgtatttctc cagggcgtcg gcaaactgac ctgctctgtc
    17461 cggctgtagt gcgttatcca gcgcctgcgc cgaattccag gtggcatccc aggcgtgaag
    17521 gatatcgcgg ccctgacgaa taaccgtttg cgaacgttca atcagtaagg cgcgttcttc
    17581 gctgtccagt tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc
    17641 cgcatgcaga ccgatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg
    17701 tgggatccgc attactttac ttaacatacc cagcgcgcca gccagttttt gcggcagcgt
    17761 gcgcgcttca ctttcaggcc agacaaaggt gtaaatcatc gcggacacca cggtaccaat
    17821 gatgataccc agggcgcgat cgcgaatttc caccaggtcg tacactgggc caaaaacgtt
    17881 ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagagcgttc
    17941 agagctggtg gcaatccatg cgcccaacag gaaaatcggt gccagcacaa acagcaattc
    18001 gacaatattg tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc
    18061 gcaaaaggcc ccgccaaaac gcagcaccat cttctggtac gacgaaccga catttggatt
    18121 agcgacgatc acgcaggtca gcatacaggt gtgaatgcct tcccagtcca cgccgctgta
    18181 aaaggtgtaa cagatcaaac aggcgagcaa tgtttttacc gcgtagcgta tatagtctgg
    18241 attggtaaaa gcatcggcga ccattgatgg cggtttggcg gcgggcgttg gcggcgtatt
    18301 cgggttcatc tggcccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg
    18361 tgccgccacc gcttcacttt cactgagccg ccagtcgctt tgccagcact gaccttcagc
    18421 aacggcatgc tgcagcttgt tgatttctga ggctaatttt tgccgaaatt caataattgc
    18481 ctgagaatca gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac
    18541 cgttgccacg cagctttgcc accatgcgct ttgagttcgc cagttggcat cgtccgcgag
    18601 acaaaagaca ttcagttttt gcagcgccag cgcctctctt tcaatccgcg tttcgggtag
    18661 cggtgcgagg ctgtccgtca ggtggctaat ggcatcatca agccgatcat taagcgcttg
    18721 atgcatttgc gtaatggcac gattgggaaa ccacagcacg ccgattaacg tcatcagcaa
    18781 ggttggatag aggccaacaa cgatacacca cagcgttaag cgcacgacta cttccggata
    18841 gtcgagcatg gcggggaagg tttgcccgta aatggcgaca gcgaaaaaga ccagtcccag
    18901 ccgatgggtg cgcatcaaaa acatgcagcc catcaggatc ggcccggcga tgatcaatcg
    18961 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19021 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19081 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag
    19141 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19201 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt
    19261 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19321 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19381 ttaatagaac gagacacttt tggcaggccg cccagcacca ggccgccgtc atccggtagc
    19441 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19501 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19561 atcacgtacc agtgacgagt gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat
    19621 tgcccgacgg aggttttgag ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc
    19681 atttccagat gtagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19741 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19801 agatccgctt ctgcggcgcg ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca
    19861 aaaccctctt tcagtaatgg ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg
    19921 gcacgggctt tttctaccgt ggcattaacc gagtcggcac caaactgttg ggcgtcaacg
    19981 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc
    20041 aggttggctt cgtacgggcg cgggtcgatg cggaacagca aatcgccctg tttcaccgcc
    20101 tggttgtcgg tgaccgccag ttctacaatg cgaccgctga cttccggcac cacatcaatg
    20161 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20221 aaaacaaggg caaccagcgc caacgcaacc actaacagag cagggaattt actgcgaggt
    20281 gcttttttcg gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag
    20341 aacagcatgg cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg
    20401 atatttgccc g
//
LOCUS       DAZPDE010000062        20343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPDE010000062 DAZPDE010000000
VERSION     DAZPDE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.062"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACHU0J_002036"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACHU0J_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1216730.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHU0J_002037"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHU0J_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1216731.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHU0J_002038"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHU0J_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1216732.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHU0J_002039"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHU0J_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1216733.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHU0J_002040"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHU0J_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACHU0J_002041"
     CDS             2795..3100
                     /locus_tag="ACHU0J_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1216734.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACHU0J_002042"
     CDS             3103..3507
                     /locus_tag="ACHU0J_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1216735.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACHU0J_002043"
     CDS             3497..3796
                     /locus_tag="ACHU0J_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1216736.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACHU0J_002044"
     CDS             3982..4278
                     /locus_tag="ACHU0J_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1216737.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHU0J_002045"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHU0J_002045"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR1216738.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACHU0J_002046"
     CDS             5627..5995
                     /locus_tag="ACHU0J_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1216739.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACHU0J_002047"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACHU0J_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR1216740.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="ACHU0J_002048"
     CDS             7203..7904
                     /locus_tag="ACHU0J_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1216741.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /gene="yhaL"
                     /locus_tag="ACHU0J_002049"
     CDS             7927..8091
                     /gene="yhaL"
                     /locus_tag="ACHU0J_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1216742.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACHU0J_002050"
     CDS             complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACHU0J_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1216743.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="ACHU0J_002051"
     CDS             complement(9641..10972)
                     /locus_tag="ACHU0J_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1216744.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="ACHU0J_002052"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="ACHU0J_002052"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1216745.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="ACHU0J_002053"
     CDS             complement(12683..13072)
                     /locus_tag="ACHU0J_002053"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1216746.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="ACHU0J_002054"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="ACHU0J_002054"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1216747.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="ACHU0J_002055"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="ACHU0J_002055"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1216748.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="ACHU0J_002056"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="ACHU0J_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1216749.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="ACHU0J_002057"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="ACHU0J_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1216750.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="ACHU0J_002058"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="ACHU0J_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1216751.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5294 a         5304 c         4844 g         4901 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taa
//
LOCUS       DAZPDE010000063        20335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPDE010000063 DAZPDE010000000
VERSION     DAZPDE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.063"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHU0J_002059"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHU0J_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1216752.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHU0J_002060"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHU0J_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR1216753.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHU0J_002061"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHU0J_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1216754.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHU0J_002062"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHU0J_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHU0J_002063"
     CDS             1631..2341
                     /locus_tag="ACHU0J_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1216755.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHU0J_002064"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHU0J_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1216756.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHU0J_002065"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHU0J_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACHU0J_002066"
     CDS             4282..6480
                     /locus_tag="ACHU0J_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR1216757.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACHU0J_002067"
     CDS             complement(6449..6889)
                     /locus_tag="ACHU0J_002067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1216758.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHU0J_002068"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACHU0J_002068"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1216759.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACHU0J_002069"
     CDS             7607..8305
                     /locus_tag="ACHU0J_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1216760.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACHU0J_002070"
     CDS             8534..9742
                     /locus_tag="ACHU0J_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216761.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACHU0J_002071"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACHU0J_002071"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1216762.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACHU0J_002072"
     tRNA            11849..11925
                     /locus_tag="ACHU0J_002072"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACHU0J_002073"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACHU0J_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1216763.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACHU0J_002074"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACHU0J_002074"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1216764.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACHU0J_002075"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACHU0J_002075"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1216765.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACHU0J_002076"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACHU0J_002076"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1216766.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACHU0J_002077"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACHU0J_002077"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1216767.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACHU0J_002078"
     CDS             complement(18540..19811)
                     /locus_tag="ACHU0J_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1216768.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..>20335
                     /locus_tag="ACHU0J_002079"
     CDS             20287..>20335
                     /locus_tag="ACHU0J_002079"
                     /inference="COORDINATES: protein motif:HMM:NF025308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1216769.1"
                     /translation="MTLAELGMAFWHDLAA"
BASE COUNT         4875 a         5035 c         5349 g         5076 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctc
//
LOCUS       DAZPDE010000064        19755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPDE010000064 DAZPDE010000000
VERSION     DAZPDE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.064"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHU0J_002080"
     CDS             <1..122
                     /locus_tag="ACHU0J_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1216770.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHU0J_002081"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHU0J_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1216771.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHU0J_002082"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHU0J_002082"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1216772.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHU0J_002083"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHU0J_002083"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1216773.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHU0J_002084"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHU0J_002084"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1216774.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHU0J_002085"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHU0J_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1216775.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHU0J_002086"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHU0J_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1216776.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHU0J_002087"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHU0J_002087"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1216777.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHU0J_002088"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHU0J_002088"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR1216778.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHU0J_002089"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHU0J_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR1216779.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHU0J_002090"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHU0J_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR1216780.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHU0J_002091"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHU0J_002091"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR1216781.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHU0J_002092"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHU0J_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR1216782.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHU0J_002093"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHU0J_002093"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR1216783.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHU0J_002094"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHU0J_002094"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1216784.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHU0J_002095"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHU0J_002095"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR1216785.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHU0J_002096"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHU0J_002096"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1216786.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..19428
                     /gene="rfbC"
                     /locus_tag="ACHU0J_002097"
     CDS             18883..19428
                     /gene="rfbC"
                     /locus_tag="ACHU0J_002097"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR1216787.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            19444..>19755
                     /locus_tag="ACHU0J_002098"
     CDS             19444..>19755
                     /locus_tag="ACHU0J_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFR1216788.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GI"
BASE COUNT         4929 a         4743 c         5355 g         4728 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atggtcaaaa atttgtagat gctgattatt ttatatgata aagaaatgta
    19441 ataatgagca taataaaaaa tagtgtctgg aacctttttg gctatgcaat accaacttta
    19501 attgctatcc cctcgctagg atttctcgct cgaggtttgg ggcctgaagg tttcggtgtt
    19561 tatacaattg caattgcact tgttgggtat gctggaattt ttgatgtagg cctgactcgc
    19621 tctgttatta gagaaattgc aattcatcgt gataatcatc atgaaagaac caaggtaatt
    19681 tcaacaagta catctttttt ggtgctattt tcatgctttg gtgctttttt attattgatt
    19741 ttctctgatg gaatt
//
LOCUS       DAZPDE010000065        19698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPDE010000065 DAZPDE010000000
VERSION     DAZPDE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.065"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHU0J_002099"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHU0J_002099"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1216789.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHU0J_002100"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHU0J_002100"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1216790.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHU0J_002101"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHU0J_002101"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1216791.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHU0J_002102"
     CDS             6038..6658
                     /locus_tag="ACHU0J_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1216792.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHU0J_002103"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHU0J_002103"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1216793.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHU0J_002104"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHU0J_002104"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1216794.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHU0J_002105"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHU0J_002105"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1216795.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHU0J_002106"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHU0J_002106"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1216796.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHU0J_002107"
     CDS             complement(10068..11069)
                     /locus_tag="ACHU0J_002107"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1216797.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHU0J_002108"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHU0J_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHU0J_002109"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHU0J_002109"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1216798.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHU0J_002110"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHU0J_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1216799.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHU0J_002111"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHU0J_002111"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1216800.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHU0J_002112"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHU0J_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1216801.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHU0J_002113"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHU0J_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1216802.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHU0J_002114"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHU0J_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1216803.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZPDE010000066        19697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPDE010000066 DAZPDE010000000
VERSION     DAZPDE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.066"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            219..1316
                     /gene="mltA"
                     /locus_tag="ACHU0J_002115"
     CDS             219..1316
                     /gene="mltA"
                     /locus_tag="ACHU0J_002115"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1216804.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1554..2360
                     /gene="tcdA"
                     /locus_tag="ACHU0J_002116"
     CDS             1554..2360
                     /gene="tcdA"
                     /locus_tag="ACHU0J_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1216805.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2411..2854)
                     /gene="csdE"
                     /locus_tag="ACHU0J_002117"
     CDS             complement(2411..2854)
                     /gene="csdE"
                     /locus_tag="ACHU0J_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1216806.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2854..4059)
                     /gene="csdA"
                     /locus_tag="ACHU0J_002118"
     CDS             complement(2854..4059)
                     /gene="csdA"
                     /locus_tag="ACHU0J_002118"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1216807.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4251..4478
                     /locus_tag="ACHU0J_002119"
     CDS             4251..4478
                     /locus_tag="ACHU0J_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1216808.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4829..5746
                     /gene="gcvA"
                     /locus_tag="ACHU0J_002120"
     CDS             4829..5746
                     /gene="gcvA"
                     /locus_tag="ACHU0J_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1216809.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5765..6160
                     /locus_tag="ACHU0J_002121"
     CDS             5765..6160
                     /locus_tag="ACHU0J_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1216810.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6153..7253
                     /gene="rlmM"
                     /locus_tag="ACHU0J_002122"
     CDS             6153..7253
                     /gene="rlmM"
                     /locus_tag="ACHU0J_002122"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1216811.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7297..8028)
                     /gene="fucR"
                     /locus_tag="ACHU0J_002123"
     CDS             complement(7297..8028)
                     /gene="fucR"
                     /locus_tag="ACHU0J_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1216812.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8086..8508)
                     /gene="fucU"
                     /locus_tag="ACHU0J_002124"
     CDS             complement(8086..8508)
                     /gene="fucU"
                     /locus_tag="ACHU0J_002124"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1216813.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8510..9929)
                     /gene="fucK"
                     /locus_tag="ACHU0J_002125"
                     /pseudo
     CDS             complement(8510..9929)
                     /gene="fucK"
                     /locus_tag="ACHU0J_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10038..11813)
                     /gene="fucI"
                     /locus_tag="ACHU0J_002126"
     CDS             complement(10038..11813)
                     /gene="fucI"
                     /locus_tag="ACHU0J_002126"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1216814.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11846..13162)
                     /gene="fucP"
                     /locus_tag="ACHU0J_002127"
     CDS             complement(11846..13162)
                     /gene="fucP"
                     /locus_tag="ACHU0J_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1216815.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13709..14356
                     /gene="fucA"
                     /locus_tag="ACHU0J_002128"
     CDS             13709..14356
                     /gene="fucA"
                     /locus_tag="ACHU0J_002128"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1216816.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14384..15532
                     /gene="fucO"
                     /locus_tag="ACHU0J_002129"
     CDS             14384..15532
                     /gene="fucO"
                     /locus_tag="ACHU0J_002129"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1216817.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15585..16340)
                     /gene="xni"
                     /locus_tag="ACHU0J_002130"
     CDS             complement(15585..16340)
                     /gene="xni"
                     /locus_tag="ACHU0J_002130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1216818.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16451..17818)
                     /gene="sdaB"
                     /locus_tag="ACHU0J_002131"
     CDS             complement(16451..17818)
                     /gene="sdaB"
                     /locus_tag="ACHU0J_002131"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1216819.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17876..19165)
                     /gene="sdaC"
                     /locus_tag="ACHU0J_002132"
     CDS             complement(17876..19165)
                     /gene="sdaC"
                     /locus_tag="ACHU0J_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1216820.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4861 a         4824 c         5198 g         4814 t
ORIGIN      
        1 ccaccccgcg tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt
       61 taccccgatc acggatttag catgattttg aacaaaaaca aaccactaaa gcctgttttt
      121 tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc
      181 ttcgttgcgc cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt
      241 accttcttat gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc
      301 gcggacagca atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag
      361 atgccgttgg cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta
      421 attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc
      481 tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg
      541 aaggtgccga caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg
      601 cgcgccatac ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg
      661 gtcgtctgcc gtctcgtgcg gagatctacg ccggggcatt gggcgataaa tacatcctcg
      721 cttacagcaa ctctctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg
      781 actttggtga tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct
      841 atcgcagcat tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga
      901 tgcaggcgat tcgtcattgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg
      961 aacagaaccc gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa
     1021 gtgcggtgcc gctggttggc cgtgcttctg ttgcctccga tcgttcaatt attccgccag
     1081 gtactacctt gctggcagaa gtgccgctgc tggataataa cggcaaattt aacggtcagt
     1141 acgaattgcg tctgatggtg gcgttggatg tcgggggcgc aatcaaaggc caacacttcg
     1201 atatctatca agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg
     1261 gacgggtttg ggtgctgaaa acggctccgg gcgcaggtaa cgtctttagc ggctgatgtg
     1321 atattctgta tgccgttttt acggatatga caggggaggg gtaacctcac atcctgatta
     1381 ttgactaagt gcgcgttgtt cataccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1441 tcatgcaaat ccaaaatatt gcaggagtta tgtaggcctg ataagcgtag cgcatcaggc
     1501 atttatgcgt ttagggtgag ggataacttc gcccttttta attctgaggt gctatgtctg
     1561 tggtaattag tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa
     1621 aagcgttgca actgtttgct gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt
     1681 cctgggcggc ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg
     1741 atgatgtgtg cgtcaccaat accaatcggc aaattcacgc tctgcgcgat aacgtcgggc
     1801 tggcaaaagc ggaagtcatg gcggagcgca ttcgccagat taacccggag tgccgtgtaa
     1861 cggtggtgga tgatttcgtg acgccggata acgtagcgca atatatgagt gccggttatt
     1921 cgtacgtgat tgatgccatt gatagtgtac ggcccaaagc ggcgctgatt gcttattgtc
     1981 ggcgcaataa aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc
     2041 agattcaggt taccgatctg gcgaaaacga ttcaggatcc gctggcggcg aagttgcgcg
     2101 agcgcctgaa aagcgatttt ggcgtagtga aaaacagtaa aggtaagctc ggcgtggatt
     2161 gcgtgttttc tactgaagcg ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga
     2221 aagccacggc agaagggccg aagcggatgg attgtgcatc tggatttggc gcggcaacga
     2281 tggtgaccgc cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga
     2341 aagcggcgcg tcagggttaa acaatcaggt cggatgagac gcgaacgtct tatccgaccc
     2401 ggttctgtgc ttaaacctgc ttcgtcgcag cgataatcgc ctcgcttaac gcatttaacc
     2461 cctggctgcg tgaggcgcta agctgcgcac gtaatcccag ctcatcaaac aatgccagcg
     2521 gcgactgtgc ctgtaattcg gcggcggttt tcccctcaac ggcagtcaac aacaccgcca
     2581 acaggccgcg cacaatgcgc ccttcgctgt tgccaaagaa gtgcattttg ccgttttcag
     2641 ccacggtata tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt
     2701 ttaactcgtc tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat
     2761 cttcccattg cgacaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc
     2821 cgaacggatg tccggcgaat tgcgggtttg tcattaatcc accaataatt ccagcgcgcg
     2881 gtcaacggca ttcaccagcg catccacatc actctttgta ttatatggcg caaatgaggc
     2941 gcgcagtgtg ccggttacgc ctaattctgc cagtagcggc tgagcgcaat gttgcccggc
     3001 ccgcaaggca ataccgtact ccgccagtag cgtcaccata tcgctgtgat gaacgccagc
     3061 aaaatcaaag gccagcaggc tggaatcctg gcagcggaat gaacgaaagc ctggacgttt
     3121 cgccagcgct tcttctgcaa gcgttgctaa gctacggctc cagctttcgg cctggttgat
     3181 atcgtaatct gccagccatt ccagcgccgc gcttaatcct atgacgccag cgacatttgg
     3241 cgttcctgct tccagtttcc acggcgcaga ttgagtggtg aaaccgtcaa aactcacttc
     3301 gtgaatcatt ttgccgccac ccagccaggg cgacatcgct tccagcagtt ctgatttgcc
     3361 atacagcacg ccgatacccg tcgggccata cagtttgtga ccagagaaag catagaaatc
     3421 gatatccagt tgctgaacat ccgcggggaa atgcaccgtc ccctgagcac catcaaccat
     3481 caccaccatc ccggctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc
     3541 agtcacgttc gacatctgac ccaacgccag aatccgacta cggggagtaa tcagctctgg
     3601 caacaaatcg acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctct
     3661 agtttgttgg gcgaccatca gccaggggac gaggttagcg tggtgttctg ccacgctgac
     3721 aataatctca tcgcccggtt gcagacgcgg acgcgcatag cattgtgcca ccatgttgat
     3781 ggattcagtg gtgccgcgcg tccagacgat agttttatca tccggtgcat tcagtaattg
     3841 cgccactttc tctcgtgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg
     3901 gctgcgatgg acgtttccgg cgctcaggct gtaaaactgt cgagtggctt caaccacggc
     3961 ttcaggttta agcgcggtcg cggcgctgtc gagatagacg cccgcatcct gtagtgcggg
     4021 aaactgggcg cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca
     4081 ccgatcgtga cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg
     4141 gatatcctgg cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag
     4201 cctgttaatc aatactatta agatttatta atcgttttaa ggagaagatg atgaaaaaga
     4261 ctgccgcaat tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga
     4321 actatgtgat gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg
     4381 ataacgatat cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc
     4441 gtactgacgt gaaagagatg gtcgaactgg aacagtaatt cgcgatcgca aggtaaaaaa
     4501 aagcaccgca attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag
     4561 gaagtgaaaa aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa
     4621 caacacaaca tcacaaccgt aagccaaaag ttcaccagaa tacgcattcc gataaaactt
     4681 ttcgttccgg ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac
     4741 aatttataat ggctcagatt aaaaaaacta ataggttaca cagtgtgatc taattgttaa
     4801 attcatttaa catcaaagtt taaaagccat gtctaaacga ttaccaccgc taaatgcctt
     4861 acgagttttt gatgccgcag cacgccattt aagttttact cgcgcagcag aagagctttt
     4921 tgtgacccag gccgcagtaa gtcatcaaat caagtctctt gaggattttt tggggctaaa
     4981 actgttccgc cgccgtaatc gttcactcct gctgaccgag gaagggcaaa gctatttcct
     5041 cgatatcaaa gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag
     5101 cgccaagggg gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc
     5161 gcgactttcc agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga
     5221 tcgtcaggaa gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa
     5281 ctggccgggg ctacgggtgg aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc
     5341 gctactgctg acaggcgaaa aacccttgaa gaccccggaa gatctggcta aacatacgtt
     5401 attacatgat gcgtcacgcc gtgactggca gacatatacc cgacagttgg ggtttaatca
     5461 tatcaacgtt cagcaagggc caatttttag tcatagcgcc atggtgctgc aagcggctat
     5521 tcacgggcag ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa tcgaggccgg
     5581 acgtcttgtt tgcccgttta atgatgttct ggtcagtaaa aatgcttttt atctggtttg
     5641 tcatgacagc caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa
     5701 agccgctgct gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac
     5761 gaccatgacc agccgtttta tgctgatttt cgccgccatt agcggcttca tttttgtggc
     5821 actgggcgct tttggtgcgc atgtgttaag taaaaccatg ggggccgttg agatgggctg
     5881 gatccaaacc ggtctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt
     5941 ggcaatgcag cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg
     6001 cacggtgctg ttcagcggca gcctttattg cctggcgctg tcccatctgc gtttgtgggc
     6061 gtttgtcaca ccggttggcg gcgtgagctt ccttgcgggc tgggcgttaa tgttagttgg
     6121 tgctatccgt ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc
     6181 cgggctttga aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt
     6241 tcggttttgc ccgcgtgaaa gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg
     6301 atgatggcga taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt
     6361 ggtttgtggt gggggaactc ctgcagcatt tgccgccaga agatcgtatt acccccattg
     6421 tcggcatgtt acagggtgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata
     6481 ccaacgaaag caaagagttg ctgaaattct gccgtaagtt cactgttccg ctacgcgctg
     6541 ccttgcgcga tgctggggtg ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg
     6601 tattctttat tgcaccagga tgctgctata ccggttactc atacagcaac aataattcgc
     6661 cgttctatat gggcattccg cgcctgaaat ttccggcaga ggcgccgagt cgttccacgc
     6721 tcaaactgga agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg
     6781 cgaacgggat gtgggcggtg gatttaggcg cttgccctgg cggctggacc taccaactgg
     6841 tgaagcgcaa catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg
     6901 ataccgggca ggtgacgtgg ctgcgggaag acggtttcaa attccgtccg acacgcagca
     6961 atatctcctg gatggtatgc gatatggttg aaaaaccggc taaagttgcg gcattgatgg
     7021 cgcagtggct ggttaatggc tggtgccgtg aaaccatttt caatctcaaa ctgccgatga
     7081 aaaaacgcta cgaagaagta tcacacaatc tggcgtatat tcaggcacag cttgatgaac
     7141 atggcataaa tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg
     7201 tgcacgtccg ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa
     7261 ggccggggcg tcgtctccgg cctgctaccc tttccgtcag gctgttacca aagaagttgc
     7321 aacctggcgc tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc
     7381 ccctgaacga ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa
     7441 tagcatcgat ttgtaatcag cgttgatcgc attggagtcc cacagtgcgc cgctgctatc
     7501 gatcccttca caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag
     7561 cgagggattg acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg
     7621 aatgcgttcg cgtttaccga gttcatggca aatcggatgg ctattggtga agacctgaat
     7681 gttgatgtca ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat
     7741 caccatccct tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata
     7801 atggcttttc agcctgatgt gaaagggatc gccactgtct tgattttgac ggtggatata
     7861 tttagcgcgt ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg
     7921 acgaatggtt tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt
     7981 atggttcagc agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc
     8041 atcattcgat aaccgcccgc gtcatgcggg cgtaccggta tgagattacg gtgttacccc
     8101 tttttttaaa agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc
     8161 tttttgcgcc cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc
     8221 ttgtagtgaa agcgcattac ggtaacgtcg ttctacttca ggatcgagag tgtcaccctc
     8281 taccgccgcc atcatcacca gcggcggggc ataactgtcc agctcaaata acgggataat
     8341 cgcctggagc aagtcgctca ccaacaggcc atcagcgcgg atcacctgcg gttccatcga
     8401 atgggcggga aagtgagcat cggaaaaaat aatttcatct ccatgtccca tctctgccag
     8461 cactttcaat agttcgggag aaattaacgg cgaaattgtt ttcagcattt cacacttcct
     8521 ctataaattc aggttcagtt tgcgggtaga aataacggta ctgataatga atctgtgcac
     8581 gggcttcttc cgggctgtta aattccccta cgccatacca accgaacagc gcagctcctg
     8641 cgacggtcgt ttcggcatcg tcgagaactt ttaccggaat atcaagcata ttggctttaa
     8701 tctgattcca caatgtgttg cgacttcctc caccgactaa caataattca taggctttaa
     8761 agtgcccgat tttttccagc atctgtagat tgcgctgtaa ttgcgcagtt aacccttcca
     8821 gcgccgcgcg atagaaatgc ccccgcgtgg tattaagcgt cactccttgc cagccagcgt
     8881 tctgacacga caataaatca cactgcattt tcacaccatc cgcgccaggt gcgatcagac
     8941 gagcttcttc aatcaacatt tgccagggtg tttcagccgt ccagaacagt tttctcaccc
     9001 attccagcac gccggatgcc agccattgca tacctgggtt atacaaccat gcctggctat
     9061 ctagttcgca ggtggaaccg gcgtactgac ttaacagcga agtatcaacc taggcgctgc
     9121 gaaccattaa aatttcccat gtaccggagg agagcacggg ttcattttgt tcggcaccag
     9181 cgccaaaaag ggcgaactgg gtatcgtgac ctgcggaaat caccggtatg ccaacgggta
     9241 agccgagcat tgctgcggcg ttgttctgta gcgtaccaat ctgttcaccc gcttccacca
     9301 gacgagggaa gagtcggcgt ggaataccgg tggcttgtaa aatttgcgga ctgaaatcgc
     9361 gttgctggat atccagcatc tggctggttc cggccatcgt gatatcagta gtgaattcgc
     9421 tggttaaacg gtggttaatc agcgacgaaa tgaagagcca ggcgtgcgcg cgttccagca
     9481 gttgtggatg attttctttc aaccacacca acttatataa cgtattgaaa ctaaaggctc
     9541 cgacgccaga aatagcctgc aaccgctgtg cggagattaa ccgttcaata ttgtccataa
     9601 ccgctgctgt tcgcggacat ttccagctaa tgatcggata gagcagattg ccttgcttat
     9661 ctaccagagc gccatccaca ctaaaggtgg tgacggcgat accgcggatg tggcattcag
     9721 tcagttcact attgatttgc cgacagcaat cagcaaagcg ttgcaaaatg gcagtctaaa
     9781 gaccactggt gccaggtgtt gttttccatc gcgatatcgc tggcattagg cgttgaggcg
     9841 cgggcaacaa tttttccctg ccgattaacc gcgatggccc tgacattggt cgcgccacag
     9901 tcgagtacca ggataacttc ttgtttcata atcgctcctg caatatagcc ggataacatt
     9961 gtttatccgg ctaaccactc ttgcttgttc aggcccggta gccggagcga ccgggcatca
    10021 catcagggtg taatgtatta acgcttgtac aacggaccgt agttctggca agcgcggtaa
    10081 tcctggcctt caatatccat gccgtgcgca gcccaggcag aaggacgata cactttggtc
    10141 tcttcaacgt tgtgcataca taccgggata cgcagcatgg aggcaagggt gataaagtct
    10201 gcgccaacgt ggccgatggt cagaactccg tggttggcac cccagttcgc cattaccgaa
    10261 tacacatccg taaacggccc tttaccagtg aggcgcggtg caaaccaggt ggttggccag
    10321 gttgagttgg tgcgtttgtt gaggatgtca tgcacatcct tcggcaattc cacgctccag
    10381 ccttccgcga tttgcagtac cggtcccagg cctttgatga tgttgacacg agtcatggtg
    10441 aacgggacgc cgccttcggt aaggaagcgg gaagagtaac cgccgccacg gaagtattcg
    10501 tggatcgccg ggcaccattc ggtagcggcg aggcaagcgt cagcctcttg ctgagagatt
    10561 tcccagtgcg gcttcatcgt cgggttacct tcgctgtcgc gttgtttaca ggaaccgtcc
    10621 agcgcagcag aacctgagtt gatcaaatgg atgatgccgt gttctgccag tccgtccagt
    10681 ttatgccccg ttacacgctc aattgcttct ggtgaccagt aggtacgcac atcggcaaat
    10741 acctgagcgg tgccggtgag ctggtgaccc attagcattg ccacgccgtt aagactgtcg
    10801 ttttcggtcg ccacgacaaa gggttcgcgc acgccattcc agtcaaatga actgttgagg
    10861 atcgcttcgg cggtgtcacc attgggatat tgatcggtcc agtgacgttg cccctggaag
    10921 cccgcagcga tggcgttgta gccaagtgat tcttccacgc gaccaatatc ggccagtttg
    10981 ctgttgcctt gcatcatgtc gcggatacac atcgccatca gtaaactttc gcgcagaact
    11041 gcgcggcttt gctcggcatt gcgttgatac tgtttgttat tttcatcttc gccatagcgg
    11101 aagtttttat cagcccaggc cagtgccatt tccagttcgg cttcgtcgta aatcttctga
    11161 tcgatacggc gacgcagttc ggtcatatcc accgcctgga ctttcattcc cagccaggat
    11221 tcaaagaagt tgtgatcaac aatagaaccg gcgataccca tcgaaacgcc gcccagcgac
    11281 agatagcttt tacctttcat gctggcgacg gccaaaccgg cgcgggcaaa gcgcagcagt
    11341 ttttcttcaa catcggcagg aatcgatgtg tcatcggcat cctgaacgtc atgaccgtaa
    11401 atggagaatg ccgggatgcc tttctggctg tgagctgcca gagccgcagc caggtaaaca
    11461 gcgccggggc gttcagtgcc gttaaagccc caaatggctt tcgggcgggt tggatccatg
    11521 tcgatggttt cactgccata gcaccagcaa ggcgttaccg taatggtgag gcctacattc
    11581 tggctgctga atttttcttc gcaagcagcg gcttcagcca tacccgcgat acaggtatcg
    11641 gaaatgacac actcgacggc agctccgcag gcatggcgca gtttctcggt cagcagtgcg
    11701 gccgtagctt tcgccatatt cattgtttgt tcttcaagcg actcacgaac acccatgcga
    11761 cgaccgtcaa taaccgggcg gataccaatt ttcggtaagc tgattttttt cattacagat
    11821 tcctcacttt attcggaaaa tatgttcagt tagttgccgt ttgagaacgg aaacgggcaa
    11881 agataaagat gaccgcgaag cagagtgcgg ggatcagttc agcagtgggg atgttgcccg
    11941 ccgcgtcact gacaaaaccc atgaccggag tgacaatacc gccgccaata atggtcataa
    12001 cgatgaagga cgaaccgtat ttggtgtcct ggccgagatt cttaatgccc agcgagaaga
    12061 ttgttgggta ctgaatcgac ataaaggcgc tgcataaagt cagggctatt aagcccacat
    12121 gaccgccagc gaaggctgag atcaggcaca gtgccatagc gattaatgcg taggcggcca
    12181 ggactttgtg tggtgcgaag cgactgatga gccaggtacc ggtgaaacga ccaataaaga
    12241 agcacaccat ggttccggtt aaatagttag cggcaaagcc tgcagtcata cctagaattt
    12301 cttctacagc gtagcgaatc aaatagctcc agcaggccgt ttgtgcgccg acatagcaga
    12361 attgcgctaa taccgcccag cgccagtgac gaatacgcgc cagacgagaa agcgatgcgg
    12421 agaacgatcc ttgtttggcg tcactgtgat tatcactctg caatgccggg aatttcgtca
    12481 gcatgatcag cagggcgacc agtaacacga tagccacgat gatcatataa ggtgtctgta
    12541 ccgataatac caggctgtgt ttatacgcac tcaattgctc tggagacatt ttatcgagaa
    12601 cgtcttgcga ttgatgtggc acgttagaca aaataagact ttgcccaaag acaaccgcga
    12661 taattgcgcc aaacgagtta aatgtttgcg caagatttaa gcggaagtga ctactgcttt
    12721 ccggccctaa taccgtaaca aaaggggttg cggcagtttc cagacaacct aatccggctg
    12781 caataataaa taggccaact aaaaataagg tgtagttcat tatttctgca gcgggccaga
    12841 ataatgcagc gcccaaggca tataaaaata atccggtaat aatccctgct ttataactga
    12901 gttttttcat caatatccca gcagggattg ggataatgaa ataaccaaag taaaaggccg
    12961 attggatcag gccagcctgg aaatttgtca gcgtaaaagc ctgctggaat tgaggtaata
    13021 aaatgtcgtt aaggttattg gctaccgccc aaagaaaaaa cagtgagcac agcagcgcga
    13081 atggaataat gtaacttctg ctttgccctg catctttatc taccgcacgg taactctgcg
    13141 tttgtattga tgtgtttccc atagcatcct cttaggttca gaagcttaag cacgtaatcg
    13201 gttatgtttt caaaagtgca aaaccagcga gttcgggctt catatcggtg aatatttgcc
    13261 tgtctgaaat gaactcatgg attcattttc aaataaaaaa cgctctgtaa tatgacggcg
    13321 gtcacactta ttagctggaa ggtgacttta tgtgactacc atcactttaa taatcgaaat
    13381 ttcatgcccg tttcagaaaa taaatcataa aaaacacaaa ccagatgacc gtattaattg
    13441 tttgtgccga aaacgggcat ttctggcgag gggaaatgga aaaatgaccg ttttgataaa
    13501 tatttttaag agcggtcatt tttaacgggg ttataaaaag agaacttgca gttatttact
    13561 gtgattagtt gagctaggtc acaaaatata agcttgtgaa tttctttctt gttaatcagg
    13621 aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca acattattgc
    13681 cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc gtcagattat
    13741 tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg ggaacgtcag
    13801 tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg aaaaactgac
    13861 ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa agctcccctc
    13921 aagcgaatgg cgtttccata tggcagccta tcaaagcaga ccggatgcca acgcggttgt
    13981 tcacaatcat gccgttcatt gcacggcagt ttccattctt aaccgatcga tccccgctat
    14041 tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt atgcgacctt
    14101 tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta aggcaacttt
    14161 gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt tatggctggc
    14221 gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta cggacccggt
    14281 gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa cctatgggtt
    14341 acggattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta acagaatgat
    14401 tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg atgaggtgaa
    14461 acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc aatgcggcgt
    14521 ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga tttacgacgg
    14581 cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat tccagaatag
    14641 cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt gtaaagcaat
    14701 tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag ggctttcccc
    14761 gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggta ctgcggcaga
    14821 agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg tttgcgttga
    14881 tccgcatgat atcccgcagg tggcgtttat tgatgctgac atgatggatg gtatgcctcc
    14941 ggcgctgaaa gccgcgacgg gtgttgatgc gctcactcat gctattgagg ggtatattac
    15001 ccgtggcgcg tgggcactaa ccgatgcact gcacattaaa gcgattgaaa tcattgctgg
    15061 ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg cgctcgggca
    15121 gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg gtatggcgca
    15181 tccactgggc gcgttttata acactccaca cggtgttgcg aacgccatcc tgttaccgca
    15241 tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg cgcgcgttat
    15301 gggcgtgaaa gtggaaggta tgagcctgga aggggcgcgt aatgccgctg ttgaagcggt
    15361 gtttgccctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg gtgtacgcaa
    15421 ggaagacatt tcggcactgg ctcaggcggc actggatgat gtttgtaccg gtggcaaccc
    15481 gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt aaatgcgctg
    15541 atgtgataat gccggatacg atttgcggcg tatccggctc gccgttaccg taccaaccgc
    15601 aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac
    15661 agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc
    15721 agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt ggcgcttttt
    15781 ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa
    15841 tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa
    15901 cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta
    15961 tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagtgtggcg
    16021 gctaagtcat cggcttcgtt gccgctggct gaccagcacg ggacgccgcg ttgctcaaag
    16081 gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg tcgatccgct
    16141 ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc atcaaaaacg
    16201 gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg ctggcaggtt
    16261 tcgacacagg gcgaaccctg aacggcatga atgcgacgga taagattcag tgcatcgaca
    16321 ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg
    16381 atgccggaac aggctatggc tggttgagaa aacaggaaga ggcttcgcaa aacgaggcct
    16441 ctgggagaga ttaatcgcag gcaacgatct tcatcgccag gccgccgcga gaggtttcgc
    16501 ggtacttggc gttcatgtct ttacctgttt cgtacatggt ttcgataact ttatcgaggc
    16561 agacgcgcgg ttcgctggta cggcgcagtg ccatacgcgc ggcgttcacc gctttcactg
    16621 ctgcaatggc gttacgctca atgcacggta cctgaacctg ccccgcgacc gggtcacaag
    16681 tcagaccgag gttgtgctcc atcgcgattt ccgctgcgat gcaaacctgc gccgggcttg
    16741 cgcctaacag ttctgccaga ccagctgccg ccatagagca cgccacgcca acttcaccct
    16801 ggcaacccac ttcggcacca gaaatcgacg cgttcatctt ataaagagaa ccaatggcgc
    16861 tggctaccag caggtaacga gccagtgagt tagcgttcac ttcgcggata aacttatcgt
    16921 agtacgccag cacagccggg ataatcccgc acgcaccgtt agtcggcgca gtcaccacgc
    16981 gaccgccagc agcgttctct tcgttcactg ccagtgcaaa catgttgatc cagtcaacaa
    17041 ccgccatcgg gtcagtggtg gttttatcct ggctgaccag catccggcgt agtgccgcag
    17101 cacggcgtgg aacgcgcagt ttgccaggca acacgccttc ggtggaaata ccgcgctcaa
    17161 taccgccacg catgacttcc cagacgttcg ccaggtgctg ttccagctct tctttactgt
    17221 gcagcgccag ctcgtttttc atcatcaggc cagagagtga cagcccggtt tcctgacaat
    17281 gtttttgcag atcggctgct gaactgtacg gataaggaac ttcaaccggt gcgctatcct
    17341 gctggccaaa atgctcttca tcaacgataa agccaccgcc aatagagtag taagtctggc
    17401 tgtaaacgac tttatcgccc gccagcgcgg taatgcgcat accgttttca tgcagagaaa
    17461 ggttgtctgc atggaagttc atgcactgat caaccgggaa ctccacttca tgctgaccgt
    17521 ttgccagcat caggcgacca tgagtattca catcctgaat aaaaccgggg atggaatcga
    17581 tatccacggt atccggcagg ttacccgcca ggcccataat aatggcgata tctgtgtggt
    17641 ggcctttacc agtcagagag agcgagccgt acacgtcaac caccacgcgg gtcacgtctt
    17701 taagcaggtt acgggcaatc agatcgtcgg taaattgttt acccgctttc attggtccaa
    17761 cggtatgaga actggaaggg ccaatgccga ttttgaaaat atcgaatacg ctaatcatag
    17821 gaaatacatc gcgttaaaac ggaggaagcg ccgcccgaaa gcggcgcgaa aggacttagc
    17881 tgaacagaga gtagaagatt gcggagattg caatcagacc catcacgaca acgaatacgt
    17941 tgctgatgtg accgctgtac ttacgcattg ccggtacttt ctgaattgcg tacatcggca
    18001 tcaggaacag gatcatcgcg atgattgggc cgcccagggt ttcaatcata cccaggatgc
    18061 tcgggttcag ggtggcaaca atccaggtcg ttaccagcat gaacagcgca gtgatacggt
    18121 tcagcttgtt gatttcgata gacttacctt taccacgcag agatttaatc accataccgt
    18181 tgaagccttc acgtgcgccc aggtagtggc cgaggaagga tttggtgata gcgataatcg
    18241 cgataatcgg agccatccac gcgataaccg gtgcgttaaa gtggttagcc aggtaagaca
    18301 gaatcgagat gttctgctct ttagccgcag ccaggtctgc cggagtcagg ctcagtacgc
    18361 agctgaagac gaagaacatt acggtcagca ccatcatgat gtgtgcgaat gccaggatct
    18421 tcgagcattt ctgttctgcc atatcgccgt actcttcacg cttcgcaacg gcgaaagaag
    18481 agatgatcgg agagtggttg aacgagaaca ccattaccgg aattgccagc cacagggtca
    18541 tccacagacc gtttccggtt gcagatgcag tgtccagaga cagcgtttcc agtgctgcgc
    18601 cgttccactg cgggatcagg tacagagcca gcagcatcag tacgccaaca aacgggaata
    18661 ccagaatact catcgcttta acgatcatct gctcaccaaa gcgaacgatg gtcatcatac
    18721 ccacgatcag gatcagcgac agaatcgcac gcggcggtgg cgtcataccc agctggtgag
    18781 acatgaagct ttcaacggta ttggtgattg ccacgctata aaccagcagg atcgggtaga
    18841 tagcgaagaa gtagagcagg gtaatcagtt tacctgcgcc aataccaaag tgttcttcta
    18901 caacctcggt gatgtcttcg cccgggtttt taccagacag tacgaagcga gtcaggccgc
    18961 ggtgagcaaa aaacgtcatc gggaacgcaa ggatagccat gatgatcagc gggatcatac
    19021 cgccaacacc ggcgttgatt ggcaggaaca gcacgcccgc gccgattgcc gtgccgtaaa
    19081 ggcccagcat ccacatggtg tctgtcttgc gccaggcact acgagagtct ttcgacgcaa
    19141 tcgtgctggt ttgagtcgtt tccatctatt tctcctggag gaatgcaaat aattcagctt
    19201 ttagccagtt cagcccggga aggtgcctga ccgtaaaaat gaaatctgtc tatcggcgat
    19261 ggcttaatca tttaccgccg taataatttg agggcggaaa gatacacaca agttatgtat
    19321 ctatcagtga tcttgatccc aaatacaata atctgcttgg aagatagatt atcccaacgc
    19381 tttactctgt aaatcaaaag taaaagagaa tttaagggca atatttgccc attaaacgac
    19441 atctgacttc ttccggggcg cgcaatatat gtaggttgaa ctgaaaaaat caatactttt
    19501 agttaatcat ggcgataatt tctttttcat agcgataaaa tacggtttat gaaaataggg
    19561 aaacgtttgt gttgcaacat aaattgtatg acatattaac tgtgatgata tttgtcgcgt
    19621 gagggtggta agaaacattc atataacaaa tgaaatgagt tgatgagtaa gtgaccaaaa
    19681 acgagaaaac ccggcaa
//
LOCUS       DAZPDE010000067        19181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPDE010000067 DAZPDE010000000
VERSION     DAZPDE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.067"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            22..2412
                     /gene="gcd"
                     /locus_tag="ACHU0J_002133"
     CDS             22..2412
                     /gene="gcd"
                     /locus_tag="ACHU0J_002133"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1216821.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2459..4009)
                     /gene="cueO"
                     /locus_tag="ACHU0J_002134"
     CDS             complement(2459..4009)
                     /gene="cueO"
                     /locus_tag="ACHU0J_002134"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1216822.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4175..4522
                     /locus_tag="ACHU0J_002135"
     CDS             4175..4522
                     /locus_tag="ACHU0J_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1216823.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4628..5494
                     /gene="speE"
                     /locus_tag="ACHU0J_002136"
     CDS             4628..5494
                     /gene="speE"
                     /locus_tag="ACHU0J_002136"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1216824.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5510..6304
                     /gene="speD"
                     /locus_tag="ACHU0J_002137"
     CDS             5510..6304
                     /gene="speD"
                     /locus_tag="ACHU0J_002137"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1216825.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6342..6704)
                     /gene="yacL"
                     /locus_tag="ACHU0J_002138"
     CDS             complement(6342..6704)
                     /gene="yacL"
                     /locus_tag="ACHU0J_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1216826.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6879..9476)
                     /gene="acnB"
                     /locus_tag="ACHU0J_002139"
     CDS             complement(6879..9476)
                     /gene="acnB"
                     /locus_tag="ACHU0J_002139"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1216827.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9831..11684
                     /locus_tag="ACHU0J_002140"
                     /pseudo
     CDS             9831..11684
                     /locus_tag="ACHU0J_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(11755..13179)
                     /gene="lpdA"
                     /locus_tag="ACHU0J_002141"
     CDS             complement(11755..13179)
                     /gene="lpdA"
                     /locus_tag="ACHU0J_002141"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1216828.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13504..15384)
                     /gene="aceF"
                     /locus_tag="ACHU0J_002142"
     CDS             complement(13504..15384)
                     /gene="aceF"
                     /locus_tag="ACHU0J_002142"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1216829.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(15399..18062)
                     /gene="aceE"
                     /locus_tag="ACHU0J_002143"
     CDS             complement(15399..18062)
                     /gene="aceE"
                     /locus_tag="ACHU0J_002143"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1216830.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18223..18987)
                     /gene="pdhR"
                     /locus_tag="ACHU0J_002144"
     CDS             complement(18223..18987)
                     /gene="pdhR"
                     /locus_tag="ACHU0J_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1216831.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4411 a         5333 c         4825 g         4612 t
ORIGIN      
        1 gaattgaaat ggtgtctctt tatggcaatt aacaatacag cctcgcgacg attactcgtc
       61 acgctaacag ccctttttgc agcgctttgc gggctgtatc tactcattgg cggaggctgg
      121 ctggtcgcga ttggcggctc ctggtactac cctatcgctg gccttgtgat gctcggcgtc
      181 gcctggatgc tgtggcgcag taaacgcgcc gcgctttggc tatacgcagc cctgctgctc
      241 ggcaccatga tttggggcgt ctgggaagtt ggtttcgact tctgggcgct gactccgcgc
      301 agcgatattc tggtcttctt cggcatctgg ctgatcctgc cgtttgtctg gcgtcgcctg
      361 gtcattcctg ccagcggcgc agttgccgca ctggtggtcg cactgctgat tagcggtggt
      421 atcctgacct gggccggatt taacgatccg caggagatca acggcacctt aagcgccgat
      481 gccacacctg ccgaagctat ctcccccgtt gccgatcagg actggcctgc gtatggccgc
      541 aatcaggaag gtcaacgctt ttcaccactg aagcaaattc acgccgataa cgtccacaag
      601 ctgaaagaag cctgggtgtt ccgtactggc gatgtgaagc agccgaacga tccgggtgaa
      661 atcaccaatg aagtgacgcc gattaaagtg ggcgataccc tttacctgtg taccgctcac
      721 cagcgcctgt ttgcgcttga tgccgccagc ggcaaagaga aatggcatta cgatcctgag
      781 ctgaaaacca acgagtcttt ccagcacgta acctgccgtg gcgtctctta tcatgaagcc
      841 aaagcagaaa cggcttcgcc ggaagtgatg gcggattgcc cgcgtcgtat cattcttcca
      901 gtgaatgatg gtcgcctgat tgcgattaac gctgaaaacg gcaaactgtg cgaaaccttc
      961 gccaataaag gcgtgctcaa tctgcaaagc aatatgccag acaccaaacc gggtctgtat
     1021 gagccgactt ctccgccaat tatcaccgat aaaaccatcg tgatggccgg ttcagtcacc
     1081 gataacttct caacccgcga aacgtctggc gtgatccgtg gttttgatgt caacaccggg
     1141 gagctgctgt gggcttttga tccgggcgcg aaagatccga acgcaatccc gtcagacgaa
     1201 cacaccttta cctttaactc accaaactcg tgggcaccag cggcctatga cgcgaagctg
     1261 gatctggtct atctgccgat gggcgtgacc acgccggata tctggggcgg taaccgcaca
     1321 ccggaacagg aacgttatgc cagctcgatt ctggcgctga atgccactac cgggaaactg
     1381 gcgtggagct accagaccgt tcaccacgac ctgtgggaca tggatcttcc ggcacagccg
     1441 acgctggcgg acatcaccgt taatggtcag aaagtgccga ttatttacgc tccggcgaaa
     1501 accggtaaca tttttgtgct cgatcgtcgt aatggcgaac tggtggttcc ggccccggaa
     1561 aaaccggttc cccaaggtgc tgccaaaggc gattacgtta cccctactca accattctcg
     1621 gaactgagct tccgtccgac gaaagatttg agcggtgcgg atatgtgggg agccaccatg
     1681 tttgaccaac tggtgtgccg cgtgatgttc caccagatgc gctatgaagg cattttcacg
     1741 ccgccatctg aacagggtac gctggtcttc ccgggtaacc tggggatgtt cgaatggggc
     1801 gggatttccg ttgatccaaa tcgtgaagtg gcaattgcca acccaatggc actgccgttt
     1861 gtttcgaaac tgctcccgcg tggtcctggc aacccgatgg agcagccgaa agatgccaaa
     1921 ggcacgggta cggaatccgg cattcagcca cagtacggtg taccgtatgg tgtcacgctc
     1981 aacccgttcc tctcaccatt tggtctgcca tgtaaacagc cagcatgggg ttatatctcg
     2041 gcgctggatc tgaaaactaa tgaagtggtg tggaagaaac gtattggtac gccgcaggac
     2101 agtatgccgt tcccgatgcc ggttccggtg ccgttcaata tgggtatgcc gatgctgggc
     2161 gggccaatct ccacggcggg taacgtgctg tttatcgccg ctacggcaga taactacctg
     2221 cgcgcttaca acatgagcaa cggtgaaaaa ctgtggcagg gtcgtttacc ggcgggtggt
     2281 caggcaacac cgatgaccta tgaagtgaat ggcaagcagt acgtggtgat ctccgcaggc
     2341 ggtcacggtt catttggtac gaagatgggc gactatattg tggcttatgc cctgccggat
     2401 gatgtgaaat aaccataatg cccggagaga tccgggcata tttccgaata cggtcttttt
     2461 ataccgtaaa ccctaacatc atccccgtat cttcatgctc cagcagatgg cagtgcgcca
     2521 tataagcatg ttctttcggt gcgttgtgat taaacttcac cagcacttca ctgacattgc
     2581 cttctacctt aacggtatct ttccagcccg cgcgatgagc cgctggcggt ttgccatttt
     2641 ctgacaagat acggaactgc gtgccgtgaa tatggaacgg atgcagcatc atgtcgccca
     2701 cgccagagat aacccaacgt tcgtactgcc ctttcgccgc cgcaaacatc ggcttattca
     2761 tatcaaacgc ctgaccgttg attttattgg catggtggaa atcgaacttc ccgccgtggt
     2821 tcatatgatt catattgccg tgccccatat ggcccatcat ctggctgtga tccattccgg
     2881 ccatcgcctg atcgccatat ttctccatta gcatctgcat ccccatcata tcgagcatcg
     2941 ggtccataga aagttgcagc ttgcgtaccg tcagcccttc cagcgaaggt aacgcaggca
     3001 ggctacttaa tgtgtcaggc aaagcaccgg aggcactaat agcaatcggc tgaatccgca
     3061 ttaccggatg cggcttgtca aacggtgcaa tcaccatccc catctggctg accggcagcg
     3121 tcaccaggtc aaagggtttg ttgtcgttaa cctccaccag cacttcaaaa cgctcgccca
     3181 tcagcaccgg cagctcgctc accttcaccg gttcaggtag cagaccaccg tcgctggcaa
     3241 tcacatacag cgggcgattg tcgctggtgg cgaaattgag cgagcgggca ttacagccat
     3301 tgagcaaacg caggcgcagc caaccacgcg gggcagcgtg ttgtgggtag attgcaccgt
     3361 tggtcagcag cgtatcgcca aaccagccca cggcggcggt catcacatcc agttgataat
     3421 caatctgccc gtcggcgcta aatttcttat cctgaacgat caccggaaca tcatcgatac
     3481 cccactgttt tggcagcatt aatttcagga tctcgtcatc ttcaatcacc accagaccag
     3541 ccagccccat cgccacctgt cgcccggtct tgccatgttg atgtggatgg aaccagcagg
     3601 tagcggcagg ttgatcaacg ttcaacgtca ccgagcgctt gccacctggc ggaataattc
     3661 cctgtgggcc gccgtcgacg tcacccggta cttccagccc gtgccagtgc aacgtcgtct
     3721 cttccgccag ttggttgtag atatcaaccg ttaccgcttt gccgcgctgt aatttcaccg
     3781 ccggccccag cagattgccg ttatagcccc aggtagttgc agttttctcg ccaaaggtgg
     3841 actgacctgc gccaatagtt aactgaatgc gattacgggc atcggtcgtg agcaaatcag
     3901 ggatcggtaa cgttgggcgt tccgccgcaa agactgcacg gctccacagt ggcaaggctg
     3961 aagccacacc cagtgcgacg gaatatttca agaaatcacg acgttgcata gttatttcct
     4021 tattcttaag caggcgaaac aaaatcaaac gttgagcata gtccttcccc ttacgggaag
     4081 gtcaagccgc agacacaatt taatcgacaa taatggtcgt cgcctcgctc gcagtgtgct
     4141 aacgtttatc ttctttaagc ccctggtaga agcaatgaag acgtttttca gaacagtgtt
     4201 attcggcagc ctgatcgccg tttgcgcaaa cagttacgcg ctcagcgagt ctgaagccga
     4261 agatatggcc gatttaacgg cagtttttgt ctttctgaag aacgattgtg gttaccagaa
     4321 cttatctaac gggcaaattc gtcgcgcact ggtctttttc gcccagcaaa accagtggga
     4381 tctcagtaat tacgacacct tcgacatgaa agctctcggt gaagacagct accgcgatct
     4441 cagcggcatt ggcattcccg tcgctaaaaa atgcaaagcc ctggcccgcg attccttaag
     4501 cctgcttgcc tacgtcaaat aatccctgat acctttttgt agaaatattg accgtgcatc
     4561 cgcggtcaat gttagctatt atgttgcgcc ctttttttac gggtgttaac aaaggaggta
     4621 tcaacccatg gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt ttgggcagta
     4681 ctttgcggta gataacgttc tgtatcatga aaagaccgat caccaggatc tgatcatttt
     4741 tgagaacgct gcatttggtc gcgtaatggc gctggatggc gtagtacaaa ccaccgagcg
     4801 cgacgagttt atctatcatg agatgatgac ccatgttccg ctactggccc acggtcacgc
     4861 gaaacatgtg ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag taacccgaca
     4921 taaaaacgtt gagtcaatca cgatggtgga aatcgatgcg ggtgtcgtgt cgttctgccg
     4981 tcagtatctg cccaaccata acgccggtag ctacgacgat ccgcgctttc aactagtgat
     5041 cgacgatggc gtcaatttcg ttaatcaaac cagccagacc tttgatgtca ttatctccga
     5101 ctgcaccgat cctatcggtc ccggcgaaag cctgttcacc tcagcatttt atgaaggctg
     5161 caaacgttgc ctgaatcctg gcggtatctt cgtcgcacaa aacggcgtct gctttttaca
     5221 gcaggaagaa gccatcgaca gccatcgcaa actcagccat tacttcagcg acgttggctt
     5281 ttatcaggcg gcgatcccga cctattacgg cggtatcatg acttttgcat gggcgacaga
     5341 taacgacgcc ttacgccatc tctcaaccga aattattcag gcgcgttttc tcgcttctgg
     5401 cctgaaatgc cgttattaca atccggcaat ccatacggca gcttttgcct tacctcagta
     5461 tctgcaagac gcactggctt cacagccgtc ctaaggagaa gataagaaat tgaaaaaact
     5521 gaaactgcat ggctttaata atctgaccaa aagtctgagt ttttgtattt acgatatctg
     5581 ctacgccaaa actgccgaag agcgcgacgg ttatattgct tatatcgatg aactctataa
     5641 tgccaaccgt ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg ccaatattct
     5701 taacatcgcc cgccaggatt acgaaccaca gggtgccagc gtcactattc tggtgagtga
     5761 agaaccggtt gacccgaaac tcatcgacaa aacagaacac cccggcccac tgccagaaac
     5821 ggtggttgct catctcgata aaagtcatat ttgcgtacat acctacccgg aaagtcatcc
     5881 tgaaggcggt ttatgtacct tccgcgccga tattgaagtc tctacctgcg gcgtgatttc
     5941 tccgctgaag gcgctgaatt acctgatcca ccagcttgag tccgatatcg taaccattga
     6001 ttatcgcgtg cgcggtttta ctcgcgacat taacggtatg aagcacttta tcgaccatga
     6061 gattaattcg attcagaact ttatgtctga cgatatgaag gcgctgtatg acatggtgga
     6121 tgtaaacgtc tatcaggaaa atatcttcca taccaagatg ttgcttaaag agttcgacct
     6181 taagcactac atgttccaca ccaaaccgga agatttaacc gacagcgagc gccaggaaat
     6241 taccgctgcg ctgtggaaag aaatgcgcga gatttattac gggcgcaata tgccagctgt
     6301 ttaacggctc tggcggagct cccaggctcc gccagattta tttacttctg ctgcacgaaa
     6361 ttgcggtaag ccgccacgac ctgcagaaaa tcctcaacgc cgcatagcga caggctttct
     6421 tcgtcgtagt agttcatccc ctcttccatt tcatcgccag cgaattccag ttgattggcg
     6481 cgaaccatca cctcttcagc gtccatccac agggtgtatt catgccctgc ccgttgccag
     6541 gaccgttcgc tacctttcag tgcgtgcgct gcttgttcca cttcatcaag caaggccagg
     6601 ttttctttca cctcttcatt aaaccagtgc ccgaccactt catgccccat ggacatacgc
     6661 acctttacca ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg ttcctctgct
     6721 cccggcaatg cgccgaaatc gcatcgcgcc attgatgtaa ttatcgcagc cttaccggag
     6781 aaaaacagcg gaacggcgat agtttagaaa taaaaaatgc ccgggaatct cattcaccgg
     6841 gcattgtgtc gtttatgcgc agcgcgtgcg cttacttatt acaccgcagt ctggaaaatc
     6901 accccatcgg ctttttcggt gtactgagaa agctggttga agttcagata acgataagta
     6961 tcaacggcag ttttatctac ttgcgcaacg taggtctggt actcttccgg cgtcggcagt
     7021 ttgccaatca gcgccgcaac agccgccagt tctgcagaag ccaggaagac attcgcgcca
     7081 gtacccaaac ggttcgggaa gttacgggta gaggtggaaa ccaccgtcgc accgtctgct
     7141 acacgcgcct ggttacccat acacagggaa cagcccggga tctcgatacg agccccgctc
     7201 ttaccgaaga cgctgtagta gccttcttcc gtcagttgcg cggcgtccat acgggttggc
     7261 ggtgccaccc acaggcgggt cggcaactga cctttgtgcg catccagcag tttaccagcg
     7321 gcacggaagt gaccgatgtt agtcatgcaa gaaccgataa atacttcgtc gatcttctcg
     7381 ccctgtaccg cagacagcgg acgtgcgtca tccggatcgt tcggagcaca caggattggc
     7441 tctttaatct ccgccagatc gatgtcgatc actgccgcgt attccgcatc tgcatcggct
     7501 tccagcagct caggattcgc cagccatttt tccatgccct gaatacggcg ttccagggta
     7561 cgacgatcgc cgtaaccttc cgcgatcatc cacttcagca ggacgatgtt ggagctcagg
     7621 tattcgatga tcggttcttt gttcagcttg atagtacaac cagcggcaga acgctcggcg
     7681 gacgcatcgg ttagctcaaa ggcctgctca actttcagat ccggcagacc ttcaatttcc
     7741 agaatgcgac cagagaagat gtttttcttg cctttcttct caacggtcag cagaccttgt
     7801 ttgatcgcat acagcggaat ggcgtgtacc agatcgcgca gggtgatgcc cggctgcatt
     7861 ttgcctttga agcgcaccag aacggattcc ggcatatcca gcggcattac gccagttgcg
     7921 gcagcaaacg ccaccagacc agaacccgcc gggaaagaga taccgatcgg gaaacgggta
     7981 tgggagtcac caccggtacc gacggtatcc ggcagcagca tacggttcag ccaggagtga
     8041 atgacgccgt cacccggacg cagcgacaca ccgccacggt tcataatgaa gtccggcagc
     8101 gtgtggtgcg tgttcacgtc aactggcttc ggatacgccg cggtgtggca gaaagactgc
     8161 atcaccaggt cagccgagaa gcccaggcac gccaggtctt tcagttcatc acgggtcatc
     8221 gggccggtgg tgtcctgaga gcctacagaa gtcattttcg gttcgcagta cgcgcccgga
     8281 cgaatgcctt tcacgccaca ggcacggcct accattttct gcgccagcga gaagccgcga
     8341 tcgctctcag cgacatcttt cgcctgacgg aacacatcac tgtgcggcag accaagtgct
     8401 tcacgcgctt tggtggtcag gccacgcccg ataatcagcg gaatacggcc gccagcacgc
     8461 acttcatcaa tcagcacgtc ggttttcagt tcgaaggtcg ccagcagttc gcctgtttcg
     8521 tggttacgca cttcaccttt gtacgggtaa acgtcaatca cgtcgcccat gttcaggtta
     8581 gagacgttga cttcgattgg cagtgcaccc gcgtcttcca tcgtgttaaa gaagatgggt
     8641 gcaattttac cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg tggaatatca
     8701 tcgcccataa accacagaac ggagttcgtg gcggatttac gcgatgaacc cgtacccaca
     8761 acgtcaccga cgtacgccag cgggaaacct ttctgttgca gagcttcaat ctgtttgatc
     8821 gggccaacaa cacctggctg gtctggctca ataccttcac gggcgttttt cagcatcgcc
     8881 agcgcgtgca gtgggatatc cgggcgtgac cacgcatccg gtgccggaga aaggtcatcg
     8941 gtgttggttt cgccagtgac tttaaagacg gtaacggtca gtttttcagc cagcgccggg
     9001 cgattcagga accattcggc atccgcccag gactgcatta cctgcttcgc atattcgttg
     9061 cctgctttcg ctttctcttc tacgtcatag aagttatcga acatcagcag cgtgtgagaa
     9121 agtgctttgg cagcaatagg tgccagtttg gcatcatcca gcgcgtcgat cagcggatga
     9181 atgttgtaac caccctgcat ggtgcccagc agttcgatag ctttttccgg agtcagcaga
     9241 ggggatttag cttcgccttt agcgatagca gccaggaagc ctgcttttac ataggcggct
     9301 tcatcgacgc ctgggggaac acggttggtt aacagatcta acaggaattc ttcttcgccc
     9361 gcgggcgggt ttttcagcag ctctacaagc gcggccattt ggtttgcatc caggggtttg
     9421 ggcgcaatcc cctcagcggc acgctcagct acgtgcttac ggtattcttc tagcacgacg
     9481 gttctcctcg ctctcattgt catagtgcgg caggcgattc tcttcacgct cctgtgagac
     9541 agcagtttgt agggtaaatg cccagtaccg caacgggcag aatagcagga ttttgacgac
     9601 gtgttaatct gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta aggatggtct
     9661 agagattgtt tccctccgta aaaacttcgt taattcgcat ggtgataatc acctttctca
     9721 acgaatccca agattagtca aaatttaaac taccgcctct ttatactcgg attcacagca
     9781 cctgcgggtg gcagttcgcc gaccattgcg atttccttga gatccgaatt atgaaaatga
     9841 ctttgccgtt taaaccccat gtgctggcgc taatttgcag tgccgggctt tgtgctgcct
     9901 ctgccgggct atatataaaa agccgcacag tggaagcgcc tgtagaaccg caatcgacac
     9961 aactggctgt gtctgacgct gccgctgtta cgcttcctgc aacggtttcc gcacctcccg
    10021 taatacccgc agtcgtcaaa tccgcattca gcactgcaca aatagatcaa tgggtcgcac
    10081 ccgtcgcgct gtatcccgac gccctacttt cgcaggtgct gatggcatca acctatccga
    10141 caaacgttgc tcaagcagtg caatggtcgc acgataatcc acttaaacaa ggcgatgctg
    10201 ctattcaggc ggtatctgac cagccgtggg acgccagcgt taaatcactg gtggcctttc
    10261 cacaattgat ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt
    10321 ttctggcgca gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac
    10381 aaaccggctc gctgaagtca tcaaccgaac agaaagttat taccacaacg aagaaagctg
    10441 taccggtaaa acagacagtc acggcacccg tcataccatc caataccgtt ttaactgcca
    10501 accccgtcat tacagagccg gcaacaaccg tcatttccat tgagcccgcc aatcctgatg
    10561 tggtctatat tcccaactac aacccaaccg tggtttacgg gaactgggcc aatactgcgt
    10621 atccgccggt ttatctgcca ccaccagccg gagaaccgtt tgttgacagc tttgtgcgcg
    10681 gattcggcta tagcatgggc gttgctacca cgtacgcact attcagcagc atcgactgag
    10741 atgacgacga tcatgaccat catcatcatg acgatgatga ttatcatcac cacgatggcg
    10801 gtcatcgtga cagtaatggc tggcaacaca acggcgacaa catcaatatc gacgtcaaca
    10861 atttcaaccg tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc
    10921 caaactaccg taatggtgtg ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa
    10981 ccgatgtcaa cggcggaatg agtgccacgc agctacctgc tccaacacgc gacagccagc
    11041 gtcaggcggc agcaagtcag tttcagcaac gaacacacgc cgcccccgtc attacacgag
    11101 atacccaacg tcaggcagcg gcacagcggt ttaatgaagc tgaacactat gggagctatg
    11161 acgacttccg cgacttcagc cgtcgccaac cactgaccca gcagcaaaag gacgccgctc
    11221 gtcagcgtta tcagtcggcc tctcctgagc agcgccaggc agtccgcgag aaaatgcaga
    11281 ctaacccgca gatccagcag cgaagggagg cggcgcgcca gcgtattcag tccgcctcgc
    11341 ctgagcagcg ccaggcggtt cgcgagaaaa tgcagactaa cccgcagaac cagcagcgaa
    11401 gagaggcagc gcgtgagcgt attcagtctg caactcccga acagcgccag gtgtttaagg
    11461 aaaaagtaca gcagcgccca ctgaaccaac agcaacgtga taacgcccgc cagcgtatac
    11521 aatcggcatc acctgaacaa cgtcaggttt ttcgggagaa agttcaggag agccgcccac
    11581 aacgtctaaa cgacagtaac catactgtca ggctgaataa cgagcaacgg tcagcagtac
    11641 gcgaacgtct ctctgaacgt ggagcaagaa gactggaaag gtaaattgca gacgtaaaaa
    11701 aagcggcgtg gttagccgct tttttaattg ccggatgttc cggcaaacga aaaattactt
    11761 cttcttcgct ttcgggttcg gcaggtcggt aatgctacct tcgaacactt ctgccgccag
    11821 gcccacagac tcgtgcagag tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc
    11881 acaacccatt tcgattgcca ggccgatttc acccagcagc tcgccgccgt tggtaccaac
    11941 aatcgcacca ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc
    12001 tgcgcagtca gaagcgatag cacgaccaga agcagcccac gggaaggtgg cggtttcata
    12061 gctgatgcct ttctctttcg cttctttctc agtcagacct acccatgcaa cttctggttc
    12121 ggtataggcg atggacggga taactttcgg atcgaagtag tgtttcttac cggcgataac
    12181 ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc agcatcggct gaccgacgat
    12241 atcgccgata gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa
    12301 accacggtcg tcaacttcca cgccagcttt gcctgcgtcg aggtttttac cgttcggcac
    12361 acgaccaatc gctaccagca cggcgtcgta acgctgcggt tcagctggtg cttttttgcc
    12421 ttccatcgtc acataaatac cgtcttcttt cgcttcaacg gcggtaactt tggtttccag
    12481 catcaggttg aatttcttgc tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc
    12541 tgccgggata acctggtcga acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta
    12601 aacggtgccc atttccagac cgatgatacc gccacccatt accagcaggc gttctggtac
    12661 ttctttcagt tccagcgcgt cagtggagtc ccagatacgc ggatcttcat gcggaataaa
    12721 cggcagttgg atcgggcgag aacccgctgc aatgatcgca ttgtcgaagt tgatcacggt
    12781 tttgccgttc tcaccttcaa cttccagggt gttagccccg gtgaatttac ccagaccgtt
    12841 gaccactttg actttgcggc ctttcgccat accagccaga ccaccggtca gctgattgat
    12901 cactttctct ttccaggtac gaatcttgtc aatatcggtt ttcggttcgc cgaagacgat
    12961 accgtgttca gccagcgctt tggcttcttc gataactttt gctacgtgca gcagtgcttt
    13021 agaagggata cagccgacgt tcaggcaaac accgccaagg gtgttgtaac gttctacgat
    13081 tacggtttcc agacctaaat cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc
    13141 cccaagtacc acgacctgag ttttgatttc agtactcatc atgacctcta tatatttatc
    13201 tccggcggtc atacccgtcg tctttcaggc cgcagcggcg ttagcttcac gccctcaccc
    13261 cagtcactta cttatgtaag ctcctgggga ttctggcgct tgctaccttg ctgcacccag
    13321 aaatccatag ggtatgtgaa gtaataccct aaccaccacc gggtcgttct atccgtcggt
    13381 attttacaaa attgttaaca atttttaaac aacaaacggc aaccgatttg tctattcgct
    13441 aataacctca atacattcat gagattacca gaaaaaagcc ggccgttggg ccggctcttt
    13501 tacttacatc accagacggc gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg
    13561 ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag
    13621 acgcggcacg aactctttac cattccacac cggctccatc gcggacttgg aaacgccgag
    13681 gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat
    13741 gctggagatg gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc
    13801 tttcttagaa atagtcatca gctcgcgaga cagctcgatg atgcctttct tgttgacgtc
    13861 tttgaatacc ggaacaacca gaccgttcgg ggtatccacc gccacaccga tgttgatgta
    13921 tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa ctattgaagc gaggcatctg
    13981 ctcaagagct gcagcaacgg ctttcatgat gaagacaacc ggggtgatct tcacatccag
    14041 cttacgtttc gccgcttctt cgttctgctg tttacggaac gcttccagct cggtgatatc
    14101 ggttttgtcg aagtgggtaa catgcgggat catcacccag ttacggctca ggttagcacc
    14161 agaaattttc tggatacggc ccagttccac ttcttcgatt tcgccaaact tgctgaagtc
    14221 caccttcggc cacggcagca tgccagggat accaccgcca gtcgccgccg gagctgcttc
    14281 tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt
    14341 acggccagtg cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca gacggcggat
    14401 cagcggggtc gcgtgaacgt aagcgtcgtt ttcagcaaat tcagatttgc cttccgcttt
    14461 cgcagccgga gcttcagctt ttgctgccgg agccggcgct gccgcttcct gtttcgcagg
    14521 agctgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgaac cagttttcac
    14581 tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag ccggaacttc
    14641 catagaagct ttatcgcctt ctacggtaat cagtgactgt tcagcggcaa ctttgtcgcc
    14701 cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac
    14761 ttctttcacg ccagctgctg gtgcaggggt cgctgccgga gtcgcttcct gtttagccgc
    14821 cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg agccggtaga
    14881 cactttgtca cccacgttca ctttgatctc tttcacggta cctgcaaacg gagccggaac
    14941 ttccatagaa gccttgtcgc cttctacggt gatcagcgac tgttcagctt caactttatc
    15001 gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt
    15061 aacgtctttt gccgccgcag ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc
    15121 tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc
    15181 gccggtctgg gttttatcgc caacagagac tttgatctct ttaacgatac ccgcctgcgg
    15241 agatggaact tccatagagg ctttgtcgcc ttctacggtg atcagcgact gttcggcttc
    15301 aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag ccccgatgtc
    15361 cggtactttg atttcgatag ccattattct tttacctctt acgccagacg cgggttaact
    15421 ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt atcgatttcg
    15481 ccacgtttag ccagttcgcc cagcgccgca accacgacat aagaagcatc aacttcgaag
    15541 tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc cagtacgcga
    15601 tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat atagtcggta
    15661 gatgccactg ccggcgcgtc gttcatcacc tgagcgatat acggaacgcg cggagtttcc
    15721 agcggatgca gcatgttcca gcgttcacaa tcctgaccat cacgcgccag ctcggtgaag
    15781 gaggtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat ctctgccgct
    15841 tcacggacgt gacgcaggat ggaaccggag cccagcagct gaactttacc tttgctacct
    15901 tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc ttccggcatt
    15961 gccggcatgt ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttt
    16021 tcaccgtaca tacgctccag accgtcatgc ataatgacag caacttcgta agcgtaagcc
    16081 gggtcgtaag agatacagtt cgggatagtc agcgactgaa tgtggctgtg accatcttcg
    16141 tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat caggaagcca
    16201 cgcgcttgct ggtctccagc cgcccagcac agatcgccga tacgctggaa gccgaacatc
    16261 gagtaataga tgtagaatgg gatcatcggc agattgttcg tgctgtaaga ggtcgccgct
    16321 gccagccagg aacaccctgc gcccagctcg ttgatccctt cctgcagaat ctgacctttc
    16381 tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc
    16441 gggctgtaaa taccaatctg acggaacaga ccttccatac cgaaagtacg cgcttcgtcg
    16501 gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcatcac gttcagagca
    16561 cgaacgaaag cgatagtggt agagagctct ttgctctgct cttccagcag cgcgccgaag
    16621 tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg gctgacggct tggcagataa
    16681 ccgtgcagtt tctgacgctg agcgtgcaga taggtatgct cttcagaacc ttccgggaag
    16741 gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa acggtcgcgg
    16801 atgtgacgca cgccgtccat gttcattttc ttaacctggt gcgcgatgtt tttaccttca
    16861 gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa ggattactgt cgctttgcct
    16921 ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg accaccacgg
    16981 ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt ttcaggatat
    17041 ttaccgaaga agtgttcacg aacgtacgca ccatctttcg atttgaaggt ctggtagtcg
    17101 ccgtcaacgg tttcgttcat cagctggatc agtttaccgc tggtatcttt acgcagcagt
    17161 tcatcccaac ggctacccca catcactttg atcacgttcc agccagcacc ttcgaagatg
    17221 ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg
    17281 ttacagttga taacgaaaac caggttatcc agtttttcac gggtagcgat ggtgatcgca
    17341 cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac ggtctgttta
    17401 gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg gtaaatagca
    17461 ccaatcggac ccagacccat agatacggtc gggaactgcc agaattccgg catcagtttc
    17521 gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt atccagctgc
    17581 tcctgagtca gacgaccttc caggaaagca cgggcgtaaa cgcccgggga gatgtggccc
    17641 tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag
    17701 cacacatcat aaatggttgc ggaagactgg aaggacgcca tgtggccgcc cagttcgagg
    17761 tcttttttcg acgcacgcag caccgtcatg atggcgttcc agcggatagc tgaacgaata
    17821 cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg gatggtgttg
    17881 atgtagttgc tgatacctgt gcctgcggct acgtttacac cgcctttgcg ggcttcagca
    17941 agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat gaccgattcg
    18001 atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa acgttctgac
    18061 atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc ccattgaact
    18121 ctcgccagaa agctcaataa gacaggttct acgtttagtt accgcgcttt tatatgcgct
    18181 tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct ttcgttgctc
    18241 cagacgacgc agagaacgct cacggcggct ctcttcacgg cttctgtcga gcaaaatttc
    18301 ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct taccgaccat
    18361 aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct cgcgacgcga
    18421 atagagcaat tcgaagttct ggcggacatt ctgggccaac atcggctcca tacaccttag
    18481 cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat actggagtac
    18541 ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg cgtggtggag
    18601 ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat aagcggcgat
    18661 accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gattcaggat ggtcggagag
    18721 cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa aagtgccgcc
    18781 accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga
    18841 gggacgggag acgtcgaact gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg
    18901 gcggagagtg ccttcgagga tcaaaaactc cagttgctgc tcaatcacat cggagagttt
    18961 tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg ccgaagtcaa
    19021 ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac cttatgtcca
    19081 tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac atatataacg
    19141 tttcaaagtt gtaactatgc acaaatgtag actttacgta g
//
LOCUS       DAZPDE010000068        19032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPDE010000068 DAZPDE010000000
VERSION     DAZPDE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.068"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHU0J_002145"
     CDS             complement(<1..1025)
                     /locus_tag="ACHU0J_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216832.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHU0J_002146"
     CDS             complement(1127..2107)
                     /locus_tag="ACHU0J_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR1216833.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHU0J_002147"
     CDS             complement(2115..2825)
                     /locus_tag="ACHU0J_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1216834.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHU0J_002148"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHU0J_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1216835.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHU0J_002149"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHU0J_002149"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1216836.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHU0J_002150"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHU0J_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1216837.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHU0J_002151"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHU0J_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1216838.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHU0J_002152"
     CDS             9402..9512
                     /locus_tag="ACHU0J_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1216839.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHU0J_002153"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHU0J_002153"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1216840.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHU0J_002154"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHU0J_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1216841.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHU0J_002155"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHU0J_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHU0J_002156"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHU0J_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1216842.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHU0J_002157"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHU0J_002157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1216843.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHU0J_002158"
     CDS             16558..17490
                     /locus_tag="ACHU0J_002158"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1216844.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACHU0J_002159"
     CDS             complement(17531..18961)
                     /locus_tag="ACHU0J_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1216845.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4654 a         4805 c         4561 g         5012 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggccag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag ga
//
LOCUS       DAZPDE010000069        18925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPDE010000069 DAZPDE010000000
VERSION     DAZPDE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.069"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHU0J_002160"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHU0J_002160"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1216846.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHU0J_002161"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHU0J_002161"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1216847.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4117..4359
                     /gene="yjbE"
                     /locus_tag="ACHU0J_002162"
     CDS             4117..4359
                     /gene="yjbE"
                     /locus_tag="ACHU0J_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1216848.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4473..5111
                     /locus_tag="ACHU0J_002163"
     CDS             4473..5111
                     /locus_tag="ACHU0J_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1216849.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5108..5845
                     /locus_tag="ACHU0J_002164"
     CDS             5108..5845
                     /locus_tag="ACHU0J_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1216850.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5845..7941
                     /locus_tag="ACHU0J_002165"
     CDS             5845..7941
                     /locus_tag="ACHU0J_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1216851.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7988..8266)
                     /locus_tag="ACHU0J_002166"
     CDS             complement(7988..8266)
                     /locus_tag="ACHU0J_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216852.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8267..8410
                     /locus_tag="ACHU0J_002167"
     CDS             8267..8410
                     /locus_tag="ACHU0J_002167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216853.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8536..8946
                     /gene="psiE"
                     /locus_tag="ACHU0J_002168"
     CDS             8536..8946
                     /gene="psiE"
                     /locus_tag="ACHU0J_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1216854.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACHU0J_002169"
     CDS             complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACHU0J_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1216855.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACHU0J_002170"
     CDS             complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACHU0J_002170"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1216856.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACHU0J_002171"
     CDS             complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACHU0J_002171"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1216857.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACHU0J_002172"
     CDS             complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACHU0J_002172"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1216858.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14994..16109
                     /gene="malK"
                     /locus_tag="ACHU0J_002173"
     CDS             14994..16109
                     /gene="malK"
                     /locus_tag="ACHU0J_002173"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1216859.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16181..17521
                     /gene="lamB"
                     /locus_tag="ACHU0J_002174"
     CDS             16181..17521
                     /gene="lamB"
                     /locus_tag="ACHU0J_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1216860.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17673..18593
                     /gene="malM"
                     /locus_tag="ACHU0J_002175"
     CDS             17673..18593
                     /gene="malM"
                     /locus_tag="ACHU0J_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1216861.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            18822..>18925
                     /locus_tag="ACHU0J_002176"
     CDS             18822..>18925
                     /locus_tag="ACHU0J_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216862.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNF"
BASE COUNT         4721 a         4881 c         4900 g         4423 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgcagatta attacttttg ttttttattt taagcttatg atttttattg ttatttaaat
     3961 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
     4021 cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
     4081 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
     4141 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
     4201 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
     4261 accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat
     4321 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
     4381 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
     4441 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
     4501 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
     4561 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
     4621 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
     4681 aagacggaca acaaaaatgg gtcactcagg atcaagccac tcttgtcaca caacatggtc
     4741 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     4801 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     4861 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     4921 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     4981 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     5041 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     5101 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     5161 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     5221 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     5281 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     5341 gctgacacgg ctggcagaac agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     5401 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     5461 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     5521 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     5581 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     5641 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     5701 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     5761 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     5821 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     5881 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     5941 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     6001 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     6061 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     6121 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     6181 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     6241 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     6301 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     6361 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     6421 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     6481 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     6541 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     6601 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     6661 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     6721 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     6781 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     6841 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     6901 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     6961 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     7021 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     7081 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     7141 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     7201 gatttgtggc tgacggacca tctactgacc accggcagcc tgtttgcaaa tcttgccaac
     7261 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     7321 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     7381 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     7441 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     7501 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     7561 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     7621 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     7681 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     7741 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     7801 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     7861 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     7921 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     7981 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     8041 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     8101 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     8161 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag
     8221 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     8281 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     8341 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     8401 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     8461 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     8521 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     8581 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     8641 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     8701 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     8761 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     8821 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     8881 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     8941 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     9001 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     9061 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     9121 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     9181 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     9241 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     9301 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     9361 ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagtg
     9421 cgccgataat ttgcagtggc ttacgaccaa atttatccac agtcataatt gccagtacgg
     9481 taaaggtgag gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg
     9541 cccccagcgt tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt
     9601 gctggaagat ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac
     9661 gaccaccggt tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg
     9721 caagcgtgtt gcccataatt ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg
     9781 acatcagcca gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag
     9841 ggatacattc cgaggcaaac atataacgcc tgccgtcagt attcagccag ctggcatcac
     9901 cggaacgggc aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa
     9961 actggttaaa tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca
    10021 ttggtgaaag cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa
    10081 attccgggac ataacctgcc agataaatag gtactgtgtt gtccgggttt atagaggtaa
    10141 aaccaagttc tggccaggca gaacctacac cagaaataaa aaacaggaca gcagaaatct
    10201 taagtgaatc acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga
    10261 tgcaaccaat cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac
    10321 ttaagttttg tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg
    10381 cggtgtcgta gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat
    10441 aactggaatt atactgggta ttcattcaga cctgccttag accattctga tcttttatca
    10501 gtgcattgat gttggaattg tcaggcagtt aaggcaatta tcatttttta caatgcgttt
    10561 acgtaatgtc ttttctgtga tcttaattgt gataattatc aaaaattgat gaaacaatac
    10621 tattgccgtg actcagagca cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa
    10681 aaataataag ttctatgatg ggaatattaa ccggcgaacg attcagattg cagacgaaag
    10741 aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa gttgggataa cgtaagttga
    10801 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
    10861 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
    10921 atcacggcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
    10981 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
    11041 tcagtaatgg cagcgataaa cgacaggatg aataccaccg ccagaatcgg tactgacagc
    11101 ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac catccagcgc agccgcttct
    11161 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
    11221 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
    11281 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
    11341 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
    11401 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc agcgaccttt
    11461 accgagttcc acagccacag cagtaccggg aatggcggtg gcgtaatgcg accatcagcc
    11521 tgttcaacgc taaaacctaa cgccagtttc cagtgatccc aggagatttg ctccgggatc
    11581 aggctaccgg tcgcgaagtt tccctgacgc agcgagatag cgacgaccat cagcagcggg
    11641 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
    11701 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
    11761 tggctttcag gttcactatc gccagcgcac ccaccagcag gaagatcagc gtggcaattg
    11821 ctgccgccag accgaagtcc tgacccccgc cgccttcaaa agcgatacgg taggtgtagt
    11881 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt
    11941 tggttaacag ctgaatcagc acgaagttgt taaagttaaa ggcgaagctg gcgatcatca
    12001 gcggcgtcag cggtttaatc agcagcggca gcgtaatctt aaagaagttc tggaacggac
    12061 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
    12121 tgcagaggat catcatgtac ggataaccca gccaggtatt gacgataatc agcatcgtgc
    12181 gagcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca
    12241 tgttgatttc accgaagctc tggttaaaca atcctttgaa aatcaagatt gaaatgaacg
    12301 atggcaccgc gtagggcaga atcagcagga cgcgatagac cgctttgccg cgcaacgctt
    12361 cccactgcac cagacatgcc agaaccatgc cgaccgccac cgttaaaaag acagtgatca
    12421 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
    12481 taaagacgcg ggtaaagttt ttccagccgg tggtcacggt gtaaccgggg cttagctttt
    12541 catcccccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
    12601 gatatttcac gccactctga ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg
    12661 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tccggcagaa
    12721 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt
    12781 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
    12841 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
    12901 cgcccgccgg gtaaagacca aagttatagg ttttgcctgc ttgccaggag cgatctaaca
    12961 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
    13021 tggcgatggt gcagaccaga gggaagagga cgaataatcc cattccagcc attcccgggt
    13081 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
    13141 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
    13201 aacccaccag caggccgagc agacctagca ctgaccattt cagcgcgtcg ctttgccacc
    13261 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
    13321 cacgccttat ccgacaacaa ctgcctgatg cgacactgat gcgtcttatc aggcctacat
    13381 gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc atccggcatt tcacagcatt
    13441 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
    13501 cggcgttgat caccgcagta cgtacggcat accagaaagc ggacatctgc gggatgttcg
    13561 gcatgatttc acctttctgg gcgttttcca tggtggcggc aatacgtgga tctttcgcca
    13621 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
    13681 ccagaccttc atcagtcagc agatagtttt cgaggaactc tttcgccagc tctttgttcg
    13741 gactggcggc gttaataccg gtgctcagca cgccaacgaa cggtttagat ggttgaccct
    13801 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
    13861 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
    13921 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
    13981 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
    14041 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
    14101 ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct
    14161 cttcccaggt ttttggcggg ttcggcagca ggtctttgtt ataaatcagc gataacgctt
    14221 caacagcgat tgggtaagca atcagcttgc cgttgtaacg tacggcatcc catgtaaacg
    14281 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
    14341 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
    14401 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
    14461 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
    14521 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
    14581 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
    14641 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacggcaac
    14701 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
    14761 attggcggta atgtggggat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
    14821 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
    14881 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
    14941 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
    15001 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
    15061 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
    15121 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
    15181 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
    15241 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
    15301 ctggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac
    15361 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg
    15421 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
    15481 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
    15541 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
    15601 aaatcgtggt gctggacgcc ggtcgcgtgg cacaggttgg gaaaccgctg gaactgtacc
    15661 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
    15721 cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
    15781 gtcaacaagt ctggctgcct gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
    15841 tgggtattcg cccggaacat ctactgccga gtgatatcgc tgacgtcatc cttgagggtg
    15901 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ctatatccag atcccttcca
    15961 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    16021 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    16081 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    16141 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    16201 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    16261 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    16321 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    16381 tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat aagagcttct atttcgacac
    16441 taacgtggcc tattccgtcg cgcaacagaa tgactgggaa gctaccgatc cggccttccg
    16501 tgaagcaaac gtgcagggaa aaaacctgat cgaatggctg ccaggttcca ccatctgggc
    16561 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    16621 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    16681 agcaacccgc tcctctgaag ctggtggttc ttcctctttt gccagcaaca atatttatga
    16741 ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa
    16801 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    16861 tcgtctggtt gatggcgcat cgaaagacgg ctggttattc actgctgaac atactcagag
    16921 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    16981 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    17041 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga
    17101 caactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    17161 caccaagtgg tggaccgttg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    17221 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    17281 gtacaaaatt accctcgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    17341 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttacg actacaacgg
    17401 cgatagcaag gttaacccga actacggcaa agccgttcct gctgatttca acggcggcag
    17461 cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    17521 atagcaaaac ctgggccgga taaggcgttt acgccgcatc cggcataaaa acaggttgtc
    17581 attatctgaa aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    17641 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    17701 tctgtttatc agcagggtta ctggcaagcg cgcctggaat tagccttgcc gatgttaact
    17761 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca
    17821 cctggacacc ggtcgatcaa tctaaaaccc agaccaccca actggcgacc ggcggccaac
    17881 aactgaacgt tcccggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    17941 gcgaactgac cctgacgctg accagcgaag tgaacaaaca aaccagcgtt tttgcgccga
    18001 acgtgctgat tcttgatcag aacatgaccc catcagcctt cttccccagc agttatttca
    18061 cctaccagga actaggcgtg atgagtgcag atcggctgga aggcgttatg cgcctgacac
    18121 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc
    18181 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc
    18241 cggatattcc cgatccggtt gctcgtcata ccagcgatgg cttactgaaa ctgaaagtca
    18301 aaacgaactc cagctccagc gtgttggtag gaccgctatt tggttcttcc gctccagctc
    18361 cggttacggt aggcaacacg gcggcaccag ctgtggctgc acctgctccg gtgccggtga
    18421 agaaaagcga gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg
    18481 ctgtcgcgaa aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg
    18541 gatcgacatc tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc
    18601 ccacagtgct gattttgcaa caactggtgc gtctcctggc gcaccttttt ttatgcttcc
    18661 ttcctgggat atgagcgatt ttttatagta cttcacttct tcttcactaa gaatgtccat
    18721 tatttcaatg cattatcaga gattcttttc ctttcgccgg tagtgtctgg acgctcaggc
    18781 tacttttcca ggttatttta tttctgtatt acagaggttt tatgataggt catatcacaa
    18841 attctggcgg caataactct ttgatgaaac atgatgtggt gcaaggaaat aatatagtag
    18901 atcttgattt actacgtaat ttcaa
//
LOCUS       DAZPDE010000070        18803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPDE010000070 DAZPDE010000000
VERSION     DAZPDE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.070"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHU0J_002177"
     CDS             complement(139..3654)
                     /locus_tag="ACHU0J_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1216863.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="ACHU0J_002178"
     CDS             complement(4107..5369)
                     /locus_tag="ACHU0J_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1216864.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="ACHU0J_002179"
     tRNA            complement(5567..5642)
                     /locus_tag="ACHU0J_002179"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="ACHU0J_002180"
     CDS             complement(5748..6455)
                     /locus_tag="ACHU0J_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1216865.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6608..6691
                     /gene="yqgH"
                     /locus_tag="ACHU0J_002181"
     CDS             6608..6691
                     /gene="yqgH"
                     /locus_tag="ACHU0J_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1216866.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="ACHU0J_002182"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="ACHU0J_002182"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1216867.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACHU0J_002183"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACHU0J_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1216868.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACHU0J_002184"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACHU0J_002184"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1216869.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="ACHU0J_002185"
     CDS             complement(11639..11914)
                     /locus_tag="ACHU0J_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1216870.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACHU0J_002186"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACHU0J_002186"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1216871.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="ACHU0J_002187"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="ACHU0J_002187"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1216872.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="ACHU0J_002188"
     CDS             13874..14200
                     /locus_tag="ACHU0J_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1216873.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="ACHU0J_002189"
     CDS             14384..15103
                     /locus_tag="ACHU0J_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1216874.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="ACHU0J_002190"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="ACHU0J_002190"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1216875.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="ACHU0J_002191"
     CDS             16442..17404
                     /locus_tag="ACHU0J_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1216876.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="ACHU0J_002192"
     CDS             complement(17505..>18803)
                     /locus_tag="ACHU0J_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR1216877.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAZPDE010000071        18410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPDE010000071 DAZPDE010000000
VERSION     DAZPDE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.071"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..618
                     /locus_tag="ACHU0J_002193"
                     /pseudo
     CDS             1..618
                     /locus_tag="ACHU0J_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            721..1044
                     /locus_tag="ACHU0J_002194"
     CDS             721..1044
                     /locus_tag="ACHU0J_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR1216878.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1111..2568)
                     /gene="nuoN"
                     /locus_tag="ACHU0J_002195"
     CDS             complement(1111..2568)
                     /gene="nuoN"
                     /locus_tag="ACHU0J_002195"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1216879.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2575..4104)
                     /gene="nuoM"
                     /locus_tag="ACHU0J_002196"
     CDS             complement(2575..4104)
                     /gene="nuoM"
                     /locus_tag="ACHU0J_002196"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1216880.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4268..6109)
                     /gene="nuoL"
                     /locus_tag="ACHU0J_002197"
     CDS             complement(4268..6109)
                     /gene="nuoL"
                     /locus_tag="ACHU0J_002197"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1216881.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6106..6408)
                     /gene="nuoK"
                     /locus_tag="ACHU0J_002198"
     CDS             complement(6106..6408)
                     /gene="nuoK"
                     /locus_tag="ACHU0J_002198"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1216882.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6405..6959)
                     /gene="nuoJ"
                     /locus_tag="ACHU0J_002199"
     CDS             complement(6405..6959)
                     /gene="nuoJ"
                     /locus_tag="ACHU0J_002199"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1216883.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6971..7513)
                     /gene="nuoI"
                     /locus_tag="ACHU0J_002200"
     CDS             complement(6971..7513)
                     /gene="nuoI"
                     /locus_tag="ACHU0J_002200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1216884.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7528..8505)
                     /gene="nuoH"
                     /locus_tag="ACHU0J_002201"
     CDS             complement(7528..8505)
                     /gene="nuoH"
                     /locus_tag="ACHU0J_002201"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1216885.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8502..11228)
                     /gene="nuoG"
                     /locus_tag="ACHU0J_002202"
     CDS             complement(8502..11228)
                     /gene="nuoG"
                     /locus_tag="ACHU0J_002202"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1216886.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11281..12618)
                     /gene="nuoF"
                     /locus_tag="ACHU0J_002203"
     CDS             complement(11281..12618)
                     /gene="nuoF"
                     /locus_tag="ACHU0J_002203"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1216887.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12615..13115)
                     /gene="nuoE"
                     /locus_tag="ACHU0J_002204"
     CDS             complement(12615..13115)
                     /gene="nuoE"
                     /locus_tag="ACHU0J_002204"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1216888.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13118..14920)
                     /gene="nuoC"
                     /locus_tag="ACHU0J_002205"
     CDS             complement(13118..14920)
                     /gene="nuoC"
                     /locus_tag="ACHU0J_002205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1216889.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15014..15676)
                     /gene="nuoB"
                     /locus_tag="ACHU0J_002206"
     CDS             complement(15014..15676)
                     /gene="nuoB"
                     /locus_tag="ACHU0J_002206"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1216890.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15692..16135)
                     /gene="nuoA"
                     /locus_tag="ACHU0J_002207"
     CDS             complement(15692..16135)
                     /gene="nuoA"
                     /locus_tag="ACHU0J_002207"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1216891.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16766..17704)
                     /gene="lrhA"
                     /locus_tag="ACHU0J_002208"
     CDS             complement(16766..17704)
                     /gene="lrhA"
                     /locus_tag="ACHU0J_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1216892.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4626 a         5108 c         4696 g         3980 t
ORIGIN      
        1 atattatttc tgttttcact ccctctgatt atttttacca accctgggtc actgcattat
       61 cgccaacgcc ccgacacgta agtcctgagc aattagaaaa aacagttcgt tatcttacac
      121 aaactgtgca tccacgtaat gccggcaata ttgataatct gaataggtcc gcagagtata
      181 taaaagagtt ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc
      241 tattacgact ctgcgcctgg aagccagaat tacccttacc cggcaatgtc ctggctttat
      301 cccgatcggg gagactttat tgctgtggtc ggcagaatgc aggatatcaa cgccgttcgt
      361 caggtaaaag cggcattatt gtcatcccgg gatttatctg tttattctat gaatgcccca
      421 gggtttattc ccggtattga tttttctgat cacctcaatt attggcaaca cgatattccc
      481 gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt gcccggtgat
      541 accgcagaca gattgaatta tcagaaaatg gctcaggtgg tagatggtgt tataaccttg
      601 ttatacaaca gtaaataata aaaatttctc gaggcgggtc cagaaacagc gacatgtaca
      661 ttttattttc cctcgtctgt tttaagatgc gaaacataaa attcaatgga ggaaaaataa
      721 atgggaatga ttggctattt tgcagaaatt gactcagaaa agattaacca actgcttgag
      781 tctactgaaa aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg
      841 gaatcactgg atcgaagtga gctgcgtaaa cagttttcca ttaagcgact caatgaaatg
      901 gagatttatc cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcctcgatc
      961 atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat
     1021 gcgctgacgg ttattgttgg gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt
     1081 cggcggcttt ctgacttaca aactaacagt ttacatcagc ggcattgcta accgcacaat
     1141 actaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag aaatcagcac
     1201 cacgataccg cccgcgctgt actgccagtt tgacggtgca tcgcgacccg gttgttccgg
     1261 tgcgtgcagg tacaggctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc
     1321 gacaaccacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta
     1381 gaacttaccg ataaagccca gcgtcatcgg gataccggcc agagacagca tcatcaccgt
     1441 catcactgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga acagggaatc
     1501 agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct
     1561 gctgaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccggtttg
     1621 cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag
     1681 acgtttgatg ttggtctggc tcaacgccat caggttaccg aagatgatgg aggcaaaggc
     1741 gataatcgcc agtactacgc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag
     1801 acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg
     1861 cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt
     1921 gaagccgagg ccaacaatca tcatgccgaa acctgccagc aacagcggct cgttgagcat
     1981 accgtcgccg aggtttttac ccagcgcgac aaacgacagg tcgccagact gcgcatacac
     2041 cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat
     2101 actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa
     2161 agagatcagt tcaataccga ggaacagaga cgccagatgg tttgcattcg ccagcaggat
     2221 cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc
     2281 ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt
     2341 gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg
     2401 gccaacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt
     2461 gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac
     2521 gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg
     2581 ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga
     2641 ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag
     2701 gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt
     2761 cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg caaagaccag
     2821 cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat
     2881 aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag
     2941 cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg
     3001 ggtatggata cgttcataaa gctgaccgca gagaataaac agacccgccg ccgacaagcc
     3061 gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagattgc
     3121 aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt
     3181 ctgagcgaag gtcatccacg caccgtagaa gatgccgata acacccagcc acatagcgat
     3241 tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca acaaaccgta
     3301 agcggcggtt ttcagcaaga tccccgcgag gtccacggaa ccggcggtcg gagcctggga
     3361 gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat
     3421 gaagaagccc agcatcaaca gatattccac accactggac attggcgtat tcagcagctc
     3481 ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat
     3541 ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt
     3601 gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg
     3661 caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc
     3721 gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat
     3781 ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca cgaccatcag
     3841 cagcgacaga ccgtcaatgg cgagatgaat agagatacca aaacgcggga tccacggcat
     3901 gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg
     3961 caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg
     4021 tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa taaagggaat
     4081 taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac
     4141 cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat
     4201 gcgccgcgaa ggcagcttat ccgacctacg ttcttatatg cccaggggca atcccacaat
     4261 ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg
     4321 atgccacata ccagcgcaga taaccgttct cgcttaacag cagaccttta cctgcaaagc
     4381 gggaaagaac cgccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg
     4441 caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaagcgt
     4501 tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag gtcaccagag
     4561 aacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag
     4621 aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc
     4681 cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca
     4741 gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga
     4801 agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg
     4861 ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga
     4921 agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga
     4981 gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat
     5041 ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag
     5101 tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc
     5161 caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg
     5221 caaaaccggc cagcagcagc gtaaccgccc cgacaatacc caccagatgc agaacttccg
     5281 gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg
     5341 ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg
     5401 tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc
     5461 acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc atatcgcgga
     5521 agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac
     5581 ccacacgggt cacgacgaac gctttcattg ctgctgcgcc attcttcgga tcggtgtaat
     5641 agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca
     5701 gcaggttatc ggcgagaacc agaactacca tgctggcgat gaacaggttg gtgtaggcga
     5761 agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tgaataagga
     5821 aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc accaggttaa
     5881 aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg tatgcctgct
     5941 cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc aggcccacag
     6001 agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca
     6061 ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc
     6121 acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag
     6181 gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc
     6241 ggtctgaccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat
     6301 ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag
     6361 aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc
     6421 gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca
     6481 cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc
     6541 gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg
     6601 tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag catgatggcc
     6661 gacaaaattg ccgggccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt
     6721 tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc
     6781 gcgtagacga taatttccag cgcaccggcg aagtaagcgc ctagtgagaa gaacaccccg
     6841 gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg
     6901 atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt
     6961 gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt
     7021 cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt
     7081 atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt
     7141 attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc
     7201 acagaccaca gaaaatgcag cgtgagaagt taatgcggaa aaattccgga taccagcgac
     7261 cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcacaga
     7321 ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac
     7381 gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga
     7441 acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt
     7501 cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt
     7561 taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacccg
     7621 gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc
     7681 ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa
     7741 cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca cgaagaacag
     7801 accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc
     7861 cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa aggtaataaa
     7921 accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga tgtcggtcat
     7981 gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta
     8041 gctcagggtc tgcgcagaag cacgcatcgc acccagcagc gagtatttgt tgttacttga
     8101 ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga agaacaaaat
     8161 cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaattg caaaggccag
     8221 cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt
     8281 cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc
     8341 gccccagcca acacggttag gtccataacg gttctggaac agacccagca ggcgacgttc
     8401 gccaaagctc atgaatgccc cacaggtgac aaccaccagc aggatcacca ccgctttgag
     8461 gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg
     8521 agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc catcggcaag
     8581 cccacctgcc ctgccgtcag tccttcggcg atttcaaccg gcagcgtgac cgtgttgcca
     8641 tcgtaactaa aggagacgcg tgtacctgcg ttcacaccca acttcgcggc atccgctggg
     8701 ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg ctgtgacaat
     8761 tcatcgctgc caaacaggtg gtagtacggc gcgatacgcc atttcccgtc ctgcggctgg
     8821 aagcgtgccg gtacgctggt gaagtaatcc agaccgtttt cgctggtttc aaacagacgc
     8881 acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc
     8941 tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg cgcggtcggc
     9001 tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc
     9061 tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg
     9121 gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacccgcc
     9181 agttctggga ttttcgccac aacagcgtca attacatggt cgagctgcgt ccagtccact
     9241 tcacggctca gcaaggtgct gtgcagcgag tgcagccagc gccagctttc cagcatgaca
     9301 gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc gcgaccttca
     9361 ttattaatta ccgtaccgtc gctttcagca aagctggcag aggaaagcac cagatgggcg
     9421 ttttccatga tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc
     9481 gcagcattca cgcgggtagc agaagcgtaa cgatgcagat cgttttccaa caccaccacc
     9541 gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgaacc gccacccata
     9601 atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg
     9661 cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat gctaccggcg
     9721 ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg
     9781 attttgtttt gcagctcagg ttcgataccg tcaaccgctg gtgcggagtt atccagcgca
     9841 tgggcaatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc
     9901 gcgatatcat ccagacgagt gtcatcaacg ttggtaacaa acagcggatg cttcgcacgt
     9961 tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgcagccatt
    10021 tcgcgcgctt tacctttcac cgcctgacgc actgccagtg cgacgcgcgc gccggtctgg
    10081 gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga
    10141 gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg
    10201 tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt
    10261 tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct
    10321 gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt
    10381 actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg accacggtcg
    10441 cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg
    10501 taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca
    10561 aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg
    10621 gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg
    10681 cgaccgaagt agacgttgtc gtgcgcgcca taaacgccca gatctgtacc gtcagcgtaa
    10741 tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg
    10801 aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg
    10861 tgtccggtca tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga
    10921 cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct
    10981 tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc
    11041 aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca
    11101 ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga
    11161 caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt
    11221 gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat cgaaattcgg
    11281 ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa
    11341 cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg
    11401 ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag
    11461 ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg cacgcagaat
    11521 tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc
    11581 gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat
    11641 cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt ccatcggcag
    11701 atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc aggctttaaa
    11761 tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg cggtggtgcc
    11821 gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca tcagcttggt
    11881 gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc
    11941 cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc atacgccgga
    12001 ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca gggagttgat
    12061 taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa
    12121 gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg cttcggcaat
    12181 ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg
    12241 gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg
    12301 ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacacagcag
    12361 gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc cagtcgaaaa
    12421 gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg
    12481 agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc ggtattcgtc
    12541 cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt
    12601 acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga
    12661 tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag
    12721 caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt gatgttcagc
    12781 tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac cacgctgtcg
    12841 caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta gaacgttgcc
    12901 acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca
    12961 tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc
    13021 gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca
    13081 aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa
    13141 caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg
    13201 ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg
    13261 tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga
    13321 aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg
    13381 tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt
    13441 tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca
    13501 gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga
    13561 tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga
    13621 tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat
    13681 aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc gctttctcgt
    13741 aagacgccag acgtttcggc atccagtcga ggaactcacg cagcagacga tcccagccgc
    13801 gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaacccg
    13861 tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg
    13921 tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg ttgatacgga
    13981 acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca
    14041 gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcaatac
    14101 ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc tccgcaccac
    14161 ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg agttgtaaaa
    14221 cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt
    14281 cctcattttc ggtgccgcgt ttcatccccc actcttccgg tttgaaggtc agggcttcca
    14341 tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgtgccg
    14401 ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt
    14461 tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca taccagttag
    14521 cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct
    14581 tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg
    14641 cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca
    14701 gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac
    14761 gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc
    14821 caaaacggtt gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg gtctgccagg
    14881 cgggttcttg cgcggttaag tcagtcatat tgttcaccat tgcaaatggt ccgtggtgac
    14941 tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc
    15001 cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac
    15061 cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca
    15121 cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca
    15181 tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat
    15241 cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca caggcaccca
    15301 ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca
    15361 ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca
    15421 atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa
    15481 cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa
    15541 ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg
    15601 ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg
    15661 tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg
    15721 tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac
    15781 gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc
    15841 ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt
    15901 cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg
    15961 agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac
    16021 cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa
    16081 agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt
    16141 actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt
    16201 catggggagg aataaaaaaa accttacaat aactgtagaa attcttttat acagctaatt
    16261 gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag cttcttcaca
    16321 ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca gatccttgac
    16381 gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac
    16441 gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga
    16501 tctgtgacac gcttttgtta ttccataaca aaaacgcagc aacaaattta cgtatttttt
    16561 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcattcgg gacgtgaatc
    16621 tctggtgatt gaaaaataaa cagttttgta cgttctgcac tatgcgagga aggattttta
    16681 ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc
    16741 tcaggaatta tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat
    16801 ccccttccgg agcagacatt gggctgtatt gccacggatt atggtagctc tccatcgcct
    16861 gatagatcac ctgggccagc tcattattac tggacgggtc atagcacagc aaatattcgg
    16921 tatcaggtag cggcggtaag ccatctactc cactcagcac acgcaggtcc gggctcatca
    16981 tctcaaccgg ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa
    17041 gcgtcgaggc gacataagcc agtcgccatg gaatatctac tttattaagc gtcgccagca
    17101 ccatatcgcg aaacggactt ggatcatcca gcaatacaag agggatcggc tcgccttttt
    17161 gcaatacgta ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct
    17221 taaacgctga aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt
    17281 ctgccatata ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac
    17341 tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag
    17401 ttaacacgcc ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca
    17461 ggattttcct ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac
    17521 cgtgacgagc gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta
    17581 cggcggactg agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa
    17641 cagcaacaaa tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta
    17701 tcatatatta tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa
    17761 caagcaattc catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt
    17821 aaaaatgcat atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa
    17881 gtagatcaca gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta
    17941 tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca
    18001 gcatcgctgg tcatacgcga acacgtacaa acagcaatgg tgaagcggca tcaagagcag
    18061 ggatccgtca tttatgcgag cattttaccc caaaaacctt ttatttataa gggtcattgc
    18121 gaattatctg atgcaaagtt atgttatgtt aggcaaagta atcttctatt attaataagc
    18181 acatcaaaac ctttttgaat attaaataat aattaattag catcatcctc attcattaat
    18241 tccgcttaac aatagtttca caccttaccc ccattgcgac acctcggaaa tcatcacgct
    18301 gttaaactct ccgtaatgcg gaaatatcat catcgcgttt cattagtgaa ttcttaaatg
    18361 aggcattttc acacaattat cttacagata aaaaaaccag acttgcaatt
//
LOCUS       DAZPDE010000072        18211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPDE010000072 DAZPDE010000000
VERSION     DAZPDE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.072"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHU0J_002209"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="ACHU0J_002209"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1216893.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHU0J_002210"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="ACHU0J_002210"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1216894.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="ACHU0J_002211"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="ACHU0J_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="ACHU0J_002212"
     CDS             complement(3183..4388)
                     /locus_tag="ACHU0J_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1216895.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHU0J_002213"
     CDS             complement(4488..4844)
                     /locus_tag="ACHU0J_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1216896.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHU0J_002214"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHU0J_002214"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHU0J_002215"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHU0J_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1216897.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHU0J_002216"
     CDS             6774..9830
                     /locus_tag="ACHU0J_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1216898.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHU0J_002217"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHU0J_002217"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1216899.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHU0J_002218"
     CDS             10337..10525
                     /locus_tag="ACHU0J_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1216900.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHU0J_002219"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHU0J_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1216901.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHU0J_002220"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHU0J_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1216902.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHU0J_002221"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHU0J_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1216903.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHU0J_002222"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHU0J_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1216904.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHU0J_002223"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHU0J_002223"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1216905.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHU0J_002224"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHU0J_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1216906.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHU0J_002225"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHU0J_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1216907.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHU0J_002226"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHU0J_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1216908.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4788 a         4401 c         4679 g         4343 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt c
//
LOCUS       DAZPDE010000073        18034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPDE010000073 DAZPDE010000000
VERSION     DAZPDE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.073"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..1078
                     /locus_tag="ACHU0J_002227"
                     /pseudo
     CDS             1..1078
                     /locus_tag="ACHU0J_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1224..2261)
                     /gene="iap"
                     /locus_tag="ACHU0J_002228"
     CDS             complement(1224..2261)
                     /gene="iap"
                     /locus_tag="ACHU0J_002228"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1216909.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2513..3421
                     /gene="cysD"
                     /locus_tag="ACHU0J_002229"
     CDS             2513..3421
                     /gene="cysD"
                     /locus_tag="ACHU0J_002229"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1216910.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3423..4850
                     /gene="cysN"
                     /locus_tag="ACHU0J_002230"
     CDS             3423..4850
                     /gene="cysN"
                     /locus_tag="ACHU0J_002230"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1216911.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4850..5455
                     /gene="cysC"
                     /locus_tag="ACHU0J_002231"
     CDS             4850..5455
                     /gene="cysC"
                     /locus_tag="ACHU0J_002231"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1216912.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5505..5828
                     /locus_tag="ACHU0J_002232"
     CDS             5505..5828
                     /locus_tag="ACHU0J_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1216913.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6022..6333
                     /gene="ftsB"
                     /locus_tag="ACHU0J_002233"
     CDS             6022..6333
                     /gene="ftsB"
                     /locus_tag="ACHU0J_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1216914.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6352..7062
                     /gene="ispD"
                     /locus_tag="ACHU0J_002234"
     CDS             6352..7062
                     /gene="ispD"
                     /locus_tag="ACHU0J_002234"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1216915.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7062..7541
                     /gene="ispF"
                     /locus_tag="ACHU0J_002235"
     CDS             7062..7541
                     /gene="ispF"
                     /locus_tag="ACHU0J_002235"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1216916.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7538..8587
                     /gene="truD"
                     /locus_tag="ACHU0J_002236"
     CDS             7538..8587
                     /gene="truD"
                     /locus_tag="ACHU0J_002236"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1216917.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8568..9329
                     /gene="surE"
                     /locus_tag="ACHU0J_002237"
     CDS             8568..9329
                     /gene="surE"
                     /locus_tag="ACHU0J_002237"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1216918.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9323..9949
                     /gene="pcm"
                     /locus_tag="ACHU0J_002238"
     CDS             9323..9949
                     /gene="pcm"
                     /locus_tag="ACHU0J_002238"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1216919.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10134..11228
                     /gene="nlpD"
                     /locus_tag="ACHU0J_002239"
     CDS             10134..11228
                     /gene="nlpD"
                     /locus_tag="ACHU0J_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1216920.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11291..12283
                     /gene="rpoS"
                     /locus_tag="ACHU0J_002240"
     CDS             11291..12283
                     /gene="rpoS"
                     /locus_tag="ACHU0J_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1216921.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12403..12810)
                     /locus_tag="ACHU0J_002241"
     CDS             complement(12403..12810)
                     /locus_tag="ACHU0J_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR1216922.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12957..13550
                     /locus_tag="ACHU0J_002242"
     CDS             12957..13550
                     /locus_tag="ACHU0J_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR1216923.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13550..14497
                     /locus_tag="ACHU0J_002243"
                     /pseudo
     CDS             13550..14497
                     /locus_tag="ACHU0J_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14770..15333)
                     /locus_tag="ACHU0J_002244"
     CDS             complement(14770..15333)
                     /locus_tag="ACHU0J_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216924.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15498..15749
                     /locus_tag="ACHU0J_002245"
     CDS             15498..15749
                     /locus_tag="ACHU0J_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1216925.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15751..17847
                     /locus_tag="ACHU0J_002246"
     CDS             15751..17847
                     /locus_tag="ACHU0J_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1216926.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4449 a         4403 c         4838 g         4344 t
ORIGIN      
        1 cgtaaatatc ctttaagttt actgaaggat aaaaatattg tgactttctt tgatttctgg
       61 ggaaaaaacc gacgtggcga gaaagagggt atcgacgact atcaccttct ttgctggcat
      121 ttgctggatg tggccgcaat gggctattta atggttaaaa gaaattgctt cgggctggct
      181 gattattttc gtcaattagg gatttctgac aaggaacagg cagctcgatt tttcgcctgg
      241 ttgctgtgct ggcacgatat tggaaaattt gcccgctctt ttcaacaact ttacctgccc
      301 cctgaattca agattcagga aggtgccaga aaaaatctct cattcaacgc tgggctactg
      361 gttgtggaat cattatttaa gtgaatgtca ggagttactt ccttcatctt cgctctctcc
      421 gcgcaaactt aggcgagtga tagaaatgtg gatgcccgtg acgacagggc atcatggtcg
      481 gccccctgat cgtatggatg aattggataa ttttctgcct gaagacaaag ctgcagcacg
      541 agattttctc cttgaaatca agccactgtt tccgcgaata gagatcccca cattctggga
      601 tgatgacgag ggcgttgaac ttataaaaca actttcctgg tatatctctg caaccgtcgt
      661 acttgcagac tggacagggt cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga
      721 tattgaagat tactggcaaa aggctttagt tcaggctcaa aacgcattaa ccgtctttcc
      781 gccacaagca gaaaccgcgc ctttcaccgg aattaatacg ctgtttcctt ttattgagaa
      841 tacgacaccg ttacagcaaa aggtgctgga tctggatatc aatcagccag gaccacagtt
      901 gtttattctg gaagatgtga ccggcgcagg taaaacagaa gcggcgctta tcctggcgca
      961 caggttgatg gctgcgggta acgctcaggg gctgtttttt ggcctgccaa caatggcaac
     1021 ggccaatgcc atgtacgatc gactggtcaa aacctggctt gctttctatt cgccagaggt
     1081 gttccccgct ccagcggaga taaaccggcg caatacggtg cggttttccc ggaagtaaac
     1141 cggacgcact ggatgcgatg atgggtatca cttagaattc cccgcccctg cggtagaact
     1201 cccagctccc attttcaaac ccatcaagac gccttcgcca gctccttcac caacggtagc
     1261 attatccgca taacgtcacg gcagcgacgt tctattcttc caggaagtgc cttatcaata
     1321 tgttgttgat tatccagtct tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt
     1381 tttgcgcgtt gctgataacc atccttattt ccaagattcc agttagtcgc ttccaccgaa
     1441 agtacagcaa tgcccgcttt gtcgaatatt tctgcgtcat tacaaccccc agtgcctttc
     1501 ggataatttt tattcaaacc cggattggtc gttgcggcta ttccgtgact gcgtgcaatt
     1561 gccagcgccc tgtcgcgcgt taatttcctt actgcttcag gggtttttac accgctgttg
     1621 aaatacaatt tatcgccaac aattaagtta tcgagattaa tcaccagcag cgtatttttc
     1681 ttttcggtgt cactcatccg cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg
     1741 ccgctggtcg ccacaaatcg aataccatac tcggtaggcg tatttttcag gcgttctgcc
     1801 agttccagca tgacacctaa acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg
     1861 ccgagattgg catctgcgtc agcatcgctc agcggggcgt aagtatccag atgcgccata
     1921 atgatgatct gctgcggcgc tttgccttca tgagcggcaa tcaccgtact tcctgtcacg
     1981 ttatgccagt tcttgcgatt atcgcgggcg gtataaatat accgactatt aaatgtccga
     2041 atatcactgc gataacccat ttgctgaaac tgttggcgaa tataatcggc agataacatt
     2101 tctgctggag ttccggtcat gcgtcccgga aagaaagtag caatatgtcg tgcctgggta
     2161 ttagcaaaat cgccaggttt aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg
     2221 agcgccaggg cagcggtacg gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca
     2281 tgcaaatttt tttaccgcca tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct
     2341 tcctaaacgc ccgaaatccg gtgccttaag cactttttga tattagcttt gccaaatcgt
     2401 tattccgtta aggaactact cattctaatt ggtaatttca ttcgttctct tacgctccct
     2461 atagtcgaaa catctgatgg caagaaaata gcggtattgc aaaggaacgg ttatggctca
     2521 aaaacgactt actcacctgc ggcaactgga ggcggaaagc atccacatta ttcgcgaggt
     2581 ggcggcagaa ttctcaaatc cggtgatgct ctactctatc ggtaaagatt ccagcgtcat
     2641 gctgcatctg gcgcgcaagg cgttttatcc aggtacgctg cctttcccgt tgctgcatgt
     2701 cgataccggc tggaaattcc gcgagatgta tgagttccgc gatcgtacgg cgaaagccta
     2761 cggctgcgag ctgctggtgc ataaaaaccc ggaaggcgtg gcgatgggga ttaatccatt
     2821 cgtgcacggc agcgcgaaac ataccgatat tatgaaaact gaaggcctga aacaggcgct
     2881 gaacaaatac ggttttgatg ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg
     2941 cgctaaagag cgaatctact ctttccgtga ccgcttccat cgctgggatc cgaaaaatca
     3001 gcgcccggag ctgtggcaca actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt
     3061 cttcccgctc tctaactgga ccgagcagga tatctggcaa tacatctggc tggaaaatat
     3121 cgacattgtg ccgctgtatc tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt
     3181 gatgatgatt gatgacaacc gtatcgacct gcaaccgggc gaagtgatca aaaaacggat
     3241 ggtgcgtttc cgtacgctgg gctgctggcc gctgaccggt gcggtggagt caaatgcaca
     3301 aacactgccg gaaatcatcg aagagatgct ggtttccacc accagtgaac gtcagggtcg
     3361 cgtgattgac cgcgaccagg cgggatccat ggagctgaaa aaacgtcagg ggtattttta
     3421 agatgaacac cgtacttgca caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga
     3481 ttgcgcaaca acataaaagc ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca
     3541 aaagtaccct gattggtcgt ctgctgcacg atacccgcca aatctacgaa gatcagctct
     3601 catcgctgca taacgacagt aagcgtcacg gaacccaggg cgaaaagctg gatctggctc
     3661 tgctggtgga cggcctgcaa gctgagcgcg aacagggcat cactattgat gtggcctacc
     3721 gctatttctc taccgagaag cgtaaattta ttatcgccga caccccaggg cacgagcagt
     3781 acactcgcaa tatggcgact ggcgcatcga catgtgaact ggcgatctta ctgattgatg
     3841 cccgtaaagg cgtgctcgat caaacccgtc gtcacagttt tatctccaca ctgttgggga
     3901 tcaaacatct ggtcgtggcg atcaacaaaa tggatctggt ggattacagt gaagagacgt
     3961 tcacccgtat tcgtgaagat tatctgacct ttgccgggca gctgccgggt aatctggata
     4021 tccgctttgt gccactctcc gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta
     4081 tgccgtggta cagcggtctg acactgctcg aagtgctgga aaccgtggag atccagcgag
     4141 tggtggatgc ccagccaatg cgcttcccgg tgcagtacgt taaccgccca aatctcgatt
     4201 ttcgtggcta cgccggaatg ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag
     4261 tgctgccctc tggtgtggaa tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg
     4321 aagaagtctt tgccggagaa gcgatcaccc tggtgctgac ggatgagatc gatatcagcc
     4381 gtggtgatct gctgctggcg gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg
     4441 atgtggtatg gatggcggaa cagccgcttt ccccgggcca gagttatgac atcaaaattg
     4501 ccggtaagaa gacgcgtgct cgtgttgatg gcattcgtta tcaggttgat attaataacc
     4561 ttacccaacg cgaagttgaa aacctgccgc tgaacggcat cggcctggtg gatctcactt
     4621 ttgacgagcc actggtgtta gatcgctatc agcaaaatcc ggttaccggt gggctgattt
     4681 ttatcgatcg cctgagcaat gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc
     4741 aggcaacagc tgcgtcatct gaattcagtg cattcgaact ggaattgaat gccctggttc
     4801 gtcgccactt tccgcactgg ggcgcgcgcg atttgctggg ggataaataa tggcgctgca
     4861 tgacgaaaac gtcgtctggc atagtcatcc ggtcactgtg caacaacgcg aactacacca
     4921 cggtcatcgt ggtgtagtgc tgtggtttac cggcctctcc gggtccggta aatcaacggt
     4981 cgccggggcg ctggaggagg cgttacataa actcagcgtc agtacgtatc tgctggatgg
     5041 cgacaatgtt cgccacggat tatgcagcga tctcggtttt agcgatgccg atcgtaaaga
     5101 gaatatccgt cgcgtcggtg aagtggcgaa tttgatggtt gaagccggac tggtggtgct
     5161 gaccgcattt atctcgccac accgcgccga acgccagatg gttcgcgaac gcgtaggaga
     5221 agggcgcttt attgaagtgt ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc
     5281 aaaaggctta tataagaaag cgcgtgccgg tgaactgcgc aactttacgg gaatagattc
     5341 cgtttacgaa gcgcctgaat cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa
     5401 tttggtacag caattattag atctgttgag acagaacgat attatcagat cctgagacac
     5461 caccgggttt ccatgcccgg ttcgtcaaag taacaggatc aaatatgcgt aatagccata
     5521 acattacact aacaaataac gacagcctta ctgaggatga agaaactaca tggtcactgc
     5581 ctggtgccgt ggtcggtttt atctcctggt tatttgcgct ggcgatgccg atgttgattt
     5641 atggctctaa cacgctgttc ttctttctct acacctggcc tttctttctg gcactgatgc
     5701 ctgtcgcggt agtggtgggg attgcgctgc attcattgat ggacggaaag ctacgctaca
     5761 gtattgtttt caccctggtg acagtaggca ttatgtttgg cgcactgttt atgtggctac
     5821 tgggctaatt tgtactttct tgtctcctct gttcataatt caaaccgtaa ctaataatga
     5881 gattatgttc tgcacgccct gggtatacgt aacaatgggc aaatgtggta catttgcccg
     5941 cgttgtcgcg gtatccccaa cagaggatgt agagtcgtct tcggatgcat gggatgatga
     6001 tgcagttttt cagggggcag gatgggtaaa ctaacgctgc tgttgctggc tattctggtc
     6061 tggctacagt attcgctgtg gttcggtaag aacggtatac atgactatac ccgcgtcaat
     6121 gatgatgtgg cggcactgca agctacaaac gcgaaactta aagcgcgaaa cgatcaactt
     6181 tttgccgaaa ttgacgatct caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat
     6241 gaactcagca tgaccaggcc gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag
     6301 cgcgcacagt ctgcggggca aaacaatcga taaatcagcc cgggaattaa catggcaacc
     6361 actcatttgg atgtttgcgc cgtggttccg gcggccggat ttggccgtcg aatgcaaacg
     6421 gaatgtccta agcaatatct ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat
     6481 gcgctgctgg cgcatccccg ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc
     6541 cgttttgcac aacttcctct ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat
     6601 gagcgtgccg attccgtgct ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg
     6661 gtgcatgacg ccgctcgtcc ttgtttgcat caggatgacc ttgcgcgatt gttggcgttg
     6721 agcgaaacca gccgcacggg agggatcctc gccgcaccag tgcgcgatac tatgaaacgt
     6781 gccgaaccgg gcaaaaatgc cattgctcat accgttgatc gcaacggctt atggcacgcg
     6841 ctgacgccgc aatttttccc tcgtgagctg ttacatgact gtctgacgcg cgctctaaat
     6901 gaaggcgcta ccattaccga cgaagcctcg gcgctggaat attgcggatt tcatccgcag
     6961 ttggtcgaag gccgcgcgga taacattaaa gtcacgcgcc cggaagattt ggcactggcc
     7021 gagttttacc tcacccgaac catccatcag gagaatacat aatgcgaatt ggacacggtt
     7081 ttgacgtaca cgcctttggc ggtgaaggcc caattatcat tggtggcgta cgcattcctt
     7141 acgaaaaagg attgctggcg cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg
     7201 cattgcttgg cgcggcggcg ctgggggata tcggcaaact gttcccggat accgatccgg
     7261 catttaaagg tgccgacagc cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga
     7321 agggttatac ccttggcaac gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc
     7381 cgcacattcc acaaatgcgc gtgtttattg ccgaagatct cggctgccat atggatgatg
     7441 ttaacgtgaa agccactact acggaaaaac ttggatttac cggacgtggg gaagggattg
     7501 cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg attgagtttg ataatctcac
     7561 ttacctccac ggtaaaccgc aaggcaccgg gctgctgaaa gccaatccgg aagactttgt
     7621 ggtggtggaa gatttgggct ttgagcctga tggtgaaggt gagcatattc tggttagaat
     7681 cctcaaaaac ggctgcaata cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat
     7741 tcattcccgt gaagtcagct tcgctgggca aaaagacaaa catgctgtaa cggaacagtg
     7801 gttatgtgct cgcgtgccgg gcaaggaaat gcccgatctg agcgcctttc aactggaagg
     7861 ctgccaggtg ctggagtatg cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg
     7921 taacgccttt accctggttc tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct
     7981 gatcgatatt tgcgtaaaag gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg
     8041 cggtagcaac ttgcagggcg cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg
     8101 caataaacgg agtttttggt tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc
     8161 tgagcgcctc aaaaaagcag acgttaatca agttgttgac ggcgatgcgc tacaattagc
     8221 cggacgtggt agctggtttg tagcaaccac cgaagaactg gcggaattac agcgtcgcgt
     8281 caacgataaa gagctgatga taaccgccgc attgccgggc agtggcgaat ggggaactca
     8341 gcgtgaagcg ctggcattcg aacaagcagc tgtcgccgca gaaactgaat tacaagcttt
     8401 actggtgcgc gaaaaagttg aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt
     8461 aagctggaac tggtgggatg acgtcaccgt agagatccgt ttctggcttc cggcgggtag
     8521 ttttgcaacc agcgttgtca gggaacttat caacacaaca ggtgattatg cgcatattgc
     8581 tgagtaatta tgacggggta catgcacccg gtatacaaac gctggcgaaa gccttgcgtg
     8641 agtttgctga cgttcaggtg gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc
     8701 tgacactgga atcctccctg cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa
     8761 tgggaacccc gaccgattgt gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg
     8821 acattgttgt gtccggaatt aacgccgggc cgaatctggg ggatgatgtt atttattccg
     8881 gtacggtagc cgccgcgatg gaaggccgtc atttaggttt tccggcgctg gccgtctcgc
     8941 ttgacgggca taaacattac gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac
     9001 tgtgtaaaga gccgctgcgc accggacgta ttcttaatat taacgttcag gatttaccct
     9061 tggatcaaat caaaggtatt cgcgtgacgc gctgcggtac acgacatccg gcagatcagg
     9121 tgatcccgca gcaagatccg cgcggcaata cgctgtactg gattggcccg ccgggcggta
     9181 aatgtgatgc cggtccgggg accgattttg ctgcggtaga tgagggctat gtctccatca
     9241 cgccgctgca tgtggattta actgcgcata gcgcgcaaga tgtggtttca gactggttaa
     9301 acagcgtggg agttggcacg caatggtaag cagacgcgta caagcacttc tggatcaatt
     9361 acgtgcgcaa ggtattcagg atgagcaggt gctgaatgca cttgccgccg tgccgcgtga
     9421 aaaattcgtt gatgaagcgt ttgaacaaaa agcctgggac aatatcgcct tgccgatagg
     9481 tcaggggcag acaatttcgc agccgtatat ggtggcgcga atgaccgaat tactcgagct
     9541 gacgccgcag tcgcgggtgc tggaaattgg cactggttcg ggatatcaaa cggcaatcct
     9601 ggcgcatctt gtccagcatg tttgctcggt tgaacggatt aaaggcttgc agtggcaggc
     9661 acgtcgccgc ctgaaaaatc ttgatttaca taatgtttca acccgtcatg gtgatggatg
     9721 gcaaggttgg caggcacgtg cgccgtttga cgctatcatt gttacggcgg caccgccgga
     9781 aattccaact gcgctaatga cgcagctgga cgaaggcggg attctcgtct tacccgtagg
     9841 ggaggagcac cagtatttga aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac
     9901 cgtggaggcc gtgcgctttg tccctttagt gaagggtgag ctggcttaaa acgtgaggaa
     9961 atacctggat ttttcctggt tattttgccg taggtcagcg tatcgtgaac atcttttcca
    10021 gtgttcagta gggtgccttg cacggtaatt atgtcactgg ttattaacca atttttcctg
    10081 ggggataaat gagcgcggga agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac
    10141 tggtttcgct atggctggca ggctgttctg acacttcaaa tccaccggca ccggtcagct
    10201 ccgttaatgg caatgcgcct gcaaatacta attctggtat gttgattacg ccgccgccga
    10261 aaatggggac gacgtctaca gcgcagcaac cgcaaattca gccggtacag cagccacaaa
    10321 ttcaggctac tcaacaaccg caaatccagc cagtgcagcc agtagctcag cagccggtac
    10381 agatggaaaa cggacgcatc gtctataacc gtcagtatgg gaacattccg aaaggcagtt
    10441 atagcggcag tacctatacc gtgaaaaaag gcgacacact tttctatatc gcctggatta
    10501 ctggcaacga tttccgtgac cttgctcagc gcaacaatat tcaggcacca tacgcgctga
    10561 acgttggtca gaccttgcag gtgggtaatg cttccggtac gccaatcact ggcggaaatg
    10621 ccattaccca ggccgacgca gcagagcaag gagttgtgat caagcctgca caaaattcca
    10681 ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt gaacagagtg
    10741 ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg
    10801 taccaacagc aagcacaacc gagccgactg tcagcagtac atcaaccagt acgcctatct
    10861 ccacctggcg ctggccgact gagggcaaag tgatcgaaac ctttggcgct tctgaggggg
    10921 gcaacaaggg gattgatatc gcaggcagca aaggacaggc aattatcgcg accgcagatg
    10981 gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg taatctgatt atcatcaaac
    11041 ataatgatga ttacctgagt gcctacgccc ataacgacac aatgctggtc cgggaacaac
    11101 aagaagtgaa ggcggggcaa aaaatagcaa ccatgggtag caccggaacc agttcaacac
    11161 gcttgcattt tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt tatttgccgc
    11221 agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag
    11281 gagccacctt atgagtcaga atacgctgaa agttcatgat ttaaatgaag atgcggaatt
    11341 tgatgagaac ggagttgagg tttttgacga aaaggcctta gtagaagagg aacccagtga
    11401 taacgatttg gccgaagagg aactgttatc gcagggagcc acacagcgtg tgttggacgc
    11461 gactcagctt taccttggtg agattggtta ttcaccactg ttaacggccg aagaagaagt
    11521 ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag
    11581 taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc tggcgttgct
    11641 ggaccttatc gaagagggca acttggggct gatccgcgcg gtagagaagt ttgacccgga
    11701 acgtggtttc cgcttctcaa catacgcaac ctggtggatt cgccagacga ttgaacgggc
    11761 gattatgaac caaacccgta ctattcgttt gccgattcac atcgtaaagg agctgaacgt
    11821 ttacctgcga accgcacgtg agttgtccca taagctggac catgaaccaa gtgcggaaga
    11881 gatcgcagag caactggata agccagttga tgacgtcagc cgtatgcttc gtcttaacga
    11941 gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt tgctggacat
    12001 cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg atatgaagca
    12061 gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg
    12121 attcggtttg ctggggtatg aagcggcaac actggaagat gtaggtcgtg aaattggcct
    12181 cacccgtgaa cgtgttcgcc agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct
    12241 gcaaacgcag gggctgaata tcgaagcgct gttccgcaag taagtaagca tctgtcagaa
    12301 aggccagtct caagcgaggc tggccttttt cttttgtttg ggaagcttgt gtttttgtcg
    12361 cctggataag acacgtcagc gtcctacccg gcaatcagca ttctatgtgc tcatcatctt
    12421 gcgcaccaac tgcataaaca gctcctgctc ttctccactc aaacgcccca aaaattcctc
    12481 atccacgctg tcaccaatag gtatcgccgc agcaagtagc ttttctcctt cggcagtcag
    12541 ccagacaaag cgtcgccgct tgtcagcgac gtcatgttca cgtctgacta agccacgatt
    12601 ttccattctt gccaacattt ctgctagcgt tgctttggtg ctgactgctg cttctatcag
    12661 tgccacctgt tcaataccag gtttatcagc aatggcgcgc atcaccgcgt actgtggttt
    12721 ggtcaggtca ggtaactcgt gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa
    12781 ctgatggaac gctttatttc gtaacgccat gtaaactccg ggctaattct ctgtcgctat
    12841 catagcggtt ttttagtgac gaagaagatg ttgttacttt tcaatccttg ccgctgtgga
    12901 aatgaaggag tatgttaata ataacgttcg tatacgaata attaagagaa taagcaatga
    12961 aactgatcgt cgggatgaca ggggctaccg gtgcgcctct tggtgtggca ttactgcaag
    13021 cgctgcgtga gatgccgaat gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca
    13081 ccattgaact ggaaacgcct tacagcgctc gcgatgttgc tgccctcgca gacttcagcc
    13141 ataacccggc ggatcaggcg gcgatcatct catccggttc ttttcgtacc gacggcatga
    13201 tcgttattcc gtgcagtatg aaaacgctcg ccggtatccg cgctggttac gccgatggcc
    13261 tggtagggcg cgcggcggac gtcgtgctca aagaaggccg caaactggtg ctggtgccgc
    13321 gtgaaatgcc gcttagcacc atccatctcg aaaatatgct cgcactttca cgcatgggcg
    13381 tggcgatggt gccgccgatg cctgcctttt ataaccatcc cgaaacggta gatgacattg
    13441 tccaccatgt ggtagcccgc gtgctggatc aatttggcct cgaacatccc cacgccaggc
    13501 gctggcaagg attgccgcag gcccggaatt tttctcagga gaatgaataa tggcatttga
    13561 tgatttacgc agctttttac aggcgcttga tgaccacggc cagttactga aaatcagcga
    13621 agaagtgaac gctgagccgg atctggcagc agcagctaac gccaccgggc gtatcggcga
    13681 cggcgcgccc gcgctgtggt ttgataatat tcgcggcttt accgatgccc gcgtggcgat
    13741 gaataccatc ggttcctggc agaaccacgc gatttccctt ggcctgccac caaacacccc
    13801 ggtcaaaaag cagattgatg agtttatccg ccgctgggat aacttcccga ttgccccgga
    13861 gcgccgcgcc aatccagcct gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt
    13921 cgatattctg ccgctgtttc gcctgaatga tggcgatggc ggtttctatc tcgacaaagc
    13981 gtgcgtggtt tcccgcgatc cgctcgaccc ggataacttc ggcaagcaga acgtcggcat
    14041 ctaccgcatg gaagtgaagg gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga
    14101 tatcgccctg catctgcata aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac
    14161 gctcggtaac gatccgatca tcacgctgat gggggccacg ccgctgaaat acgatcagtc
    14221 tgagtatgaa atggcaggcg cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct
    14281 gaccggtttt gatgtgccgt ggggttcaga agtgatcctc gaaggggtta tcgaaagccg
    14341 taaacgcgaa atcgaagggc cgttcggtga gtttaccggg cactactccg gtgggcgcaa
    14401 catgaccgtg gtgcgcatcg ataaagtctc ttatcgcagc aaaccgattt tcgaatcact
    14461 ctatctcggt atgccgtgga ccgaaatcga ctacctatga ctaggtaatt tattcaagat
    14521 aaccgggaag agattaaaaa agacgggaat agattggaat caacaagtta ggaactcaag
    14581 gttttcaaga taaagcgagt ccaaaaatgt taaacggttc gatatcaata aaacattcaa
    14641 gataactcac aggttcttac aatagccttt aaagaccgtt caaaaacgat tcaggtgttt
    14701 attatagcgc tgatgggtgc aagcgccaaa accaatgtaa ttttagcaca gctaggcact
    14761 tgcactgggt tatgcgctgg ctgctttctg aggtagattt aagtgatcgc ccttagcttc
    14821 ggacaacgcg taaatctctc taaccttttc gtctggcaaa ttaccaaggc ttaacgctat
    14881 ggctctacga actccaacta gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag
    14941 taattgctcc gctccttttc tcccaagaac atcagctact tgttgttttt cttgtggttt
    15001 aagaaatgac aagaacatta gaatttgagc atcaacatta tccgcattct cttttttggg
    15061 ttctttggaa tcttttagtt caacatcacc ctctcccgtc atgagccatt ccaaggtaac
    15121 attttcgctt tctgctatct tttttaactg atacgggcgt ggtatcggtg cgtaatcccg
    15181 cctgttgtaa taatttttta aagtgccttc attgatcccc catgcccgag cagccgcatt
    15241 tcttgaaggg tatcgttcta ctaacctaaa gattctttcg ataatcggtt cttttccatc
    15301 ggcaggaaaa gaaccttggg gtgcttttct cattcttggt tcctttctat ctaattcatt
    15361 gataaataat aaaattaaaa aataaaccat aaaaacacca aagaaccctt tgcagggaat
    15421 ctttggtgtc ttaatcttta tccatacgga tatccgaccg gatatccgcg cgggtaattt
    15481 tgtaggttaa aggaaagatg agacaaagag aagatagtga ccgcgattgg ccttcgctga
    15541 agattcagct ggcgctcaaa gataacgggt ggtcttcctt ccgggagctt tcactccatg
    15601 ctggttatgc tcaggattca ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac
    15661 gcgtaatttc tgacgctata ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta
    15721 acgatggatt tatcaggaag gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg
    15781 ctcggcgtac caggaatgcc agccaccaca aaaggcatcc gacaggcgtt acagcgtttt
    15841 tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca caaaagccat tgagtaccac
    15901 attgattgtc tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt ggaacagctg
    15961 gtggctacag agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct
    16021 gacgctgtcc aggcaatcga agcatatcgc ggttctccac aactgatgga agaacgcctg
    16081 aatgcgctga ccgaaaacca gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa
    16141 gtgctgaagc tggaaagcgc cggtaacccc gggcgactga aagccattaa ctttcttgtt
    16201 gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac aggccgcagt taacgccaat
    16261 gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac tttaccagtg ggttctgaaa
    16321 tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac
    16381 gaaataaaac cggaagaaat cagctggctg cctgaatttc tggcgcagta tcgccaggtg
    16441 aacggcagac ccatgtcaga agcctacgag gattttgtcg cagaatggca gcgtcgccat
    16501 gcggacgaac cgtacatgct ggaggtgatg ccttcctatg atgttgtccg gtatgccatg
    16561 aaaaaactgc cggaagtggt gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag
    16621 cttgaaggat tcacccgccg cgactggaca gctatgccgg tgaattatgt ctggattggt
    16681 gacggtcacg gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc attctcaccg
    16741 gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg ttggctggag tcttgacctt
    16801 gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa
    16861 ccgttcctgt attactcgga taacggttcc ggtgaaacgg ctgacatgct ggacaaagag
    16921 gtcgtgggta ttctgccacg cctggggatt aaacacccta ccggtattgc cggtaatcca
    16981 cagggacgcg gcattattga acgcctgaac cggacactgc cgatgcgtat agcgaggagg
    17041 tatcgcacct attttggcaa gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat
    17101 ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg
    17161 gcaatgcgcg agctgccatc gtgggcggag cttattgagg ctattcgtga aggcgtggaa
    17221 tggtacaaca accgcccgca ttctgagctg ccgatgaaac cgaacggacg gcattacagc
    17281 ccgacagagt tcagaaaaaa acgccaggca gaagaagaca ccgaaattga gtggctgagt
    17341 gatcttgagc tgcgcgacat gttccgccca atggtggaac gaccggtaag acgctgcgaa
    17401 attcagtggc tgaacaatat ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa
    17461 gtgcttatca gttatgacat tcatgacgcc gagcgaatta cggtacgtcg caaggacggc
    17521 agctttatct gtgaggcgat atggaacggc aataaacgcg cagcctttgc tgtcagtgct
    17581 gaatatcaca aacaacagca gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt
    17641 cgtgatgctg aagatgaagg tattcagatt ctggaacaca aacaggctga accctggctg
    17701 agtaatgttt atcgcccggt tggtaatgtg gtggcagttc agcaaccgga atatgaagaa
    17761 gagcatgacg aagaatttga acgcgatttc cggctgggga tgcaaaaact ttttgccatg
    17821 caggaagaag atgatccgct ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa
    17881 ataaaggaaa caaagctgat ttgaaataac cgaggaaata ctatgactga tattgatgat
    17941 gtttgaggtg tactggcaat agcggacact accatttgtt ctttttttaa gcagccatct
    18001 gatgatattt ttccctgaag gctgccgggg agat
//
LOCUS       DAZPDE010000074        18003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPDE010000074 DAZPDE010000000
VERSION     DAZPDE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.074"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHU0J_002247"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHU0J_002247"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1216927.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHU0J_002248"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHU0J_002248"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1216928.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHU0J_002249"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHU0J_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1216929.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHU0J_002250"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHU0J_002250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1216930.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHU0J_002251"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHU0J_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHU0J_002252"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHU0J_002252"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1216931.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHU0J_002253"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHU0J_002253"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1216932.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHU0J_002254"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHU0J_002254"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1216933.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHU0J_002255"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHU0J_002255"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1216934.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHU0J_002256"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHU0J_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1216935.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHU0J_002257"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHU0J_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1216936.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHU0J_002258"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHU0J_002258"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1216937.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHU0J_002259"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHU0J_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1216938.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHU0J_002260"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHU0J_002260"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1216939.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHU0J_002261"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHU0J_002261"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1216940.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHU0J_002262"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHU0J_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1216941.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
BASE COUNT         4216 a         4567 c         4831 g         4389 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccggt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tat
//
LOCUS       DAZPDE010000075        17924 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPDE010000075 DAZPDE010000000
VERSION     DAZPDE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.075"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHU0J_002263"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHU0J_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1216942.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHU0J_002264"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHU0J_002264"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1216943.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHU0J_002265"
     CDS             2826..3980
                     /locus_tag="ACHU0J_002265"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1216944.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHU0J_002266"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHU0J_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1216945.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHU0J_002267"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHU0J_002267"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1216946.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHU0J_002268"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHU0J_002268"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1216947.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHU0J_002269"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHU0J_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1216948.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHU0J_002270"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHU0J_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1216949.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHU0J_002271"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHU0J_002271"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1216950.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHU0J_002272"
     CDS             11296..11511
                     /locus_tag="ACHU0J_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1216951.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHU0J_002273"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHU0J_002273"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1216952.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHU0J_002274"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHU0J_002274"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1216953.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHU0J_002275"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHU0J_002275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1216954.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHU0J_002276"
     CDS             complement(16247..16786)
                     /locus_tag="ACHU0J_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1216955.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHU0J_002277"
     CDS             complement(16802..17320)
                     /locus_tag="ACHU0J_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1216956.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="ACHU0J_002278"
     CDS             complement(17631..17822)
                     /locus_tag="ACHU0J_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1216957.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..>17924
                     /locus_tag="ACHU0J_002279"
     CDS             17840..>17924
                     /locus_tag="ACHU0J_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216958.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASV"
BASE COUNT         4213 a         4413 c         4951 g         4347 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtgg
//
LOCUS       DAZPDE010000076        17922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPDE010000076 DAZPDE010000000
VERSION     DAZPDE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.076"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            8..562
                     /locus_tag="ACHU0J_002280"
     CDS             8..562
                     /locus_tag="ACHU0J_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1216959.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(572..1999)
                     /locus_tag="ACHU0J_002281"
     CDS             complement(572..1999)
                     /locus_tag="ACHU0J_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1216960.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1996..2703)
                     /locus_tag="ACHU0J_002282"
     CDS             complement(1996..2703)
                     /locus_tag="ACHU0J_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1216961.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2867..3844
                     /gene="ybeQ"
                     /locus_tag="ACHU0J_002283"
     CDS             2867..3844
                     /gene="ybeQ"
                     /locus_tag="ACHU0J_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1216962.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3914..4396)
                     /locus_tag="ACHU0J_002284"
     CDS             complement(3914..4396)
                     /locus_tag="ACHU0J_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1216963.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4631..7213
                     /gene="leuS"
                     /locus_tag="ACHU0J_002285"
     CDS             4631..7213
                     /gene="leuS"
                     /locus_tag="ACHU0J_002285"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1216964.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7228..7809
                     /gene="lptE"
                     /locus_tag="ACHU0J_002286"
     CDS             7228..7809
                     /gene="lptE"
                     /locus_tag="ACHU0J_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1216965.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7809..8840
                     /gene="holA"
                     /locus_tag="ACHU0J_002287"
     CDS             7809..8840
                     /gene="holA"
                     /locus_tag="ACHU0J_002287"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1216966.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8842..9483
                     /gene="nadD"
                     /locus_tag="ACHU0J_002288"
     CDS             8842..9483
                     /gene="nadD"
                     /locus_tag="ACHU0J_002288"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1216967.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9507..10118
                     /locus_tag="ACHU0J_002289"
     CDS             9507..10118
                     /locus_tag="ACHU0J_002289"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR1216968.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10378..10695
                     /gene="rsfS"
                     /locus_tag="ACHU0J_002290"
     CDS             10378..10695
                     /gene="rsfS"
                     /locus_tag="ACHU0J_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1216969.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10699..11166
                     /gene="rlmH"
                     /locus_tag="ACHU0J_002291"
     CDS             10699..11166
                     /gene="rlmH"
                     /locus_tag="ACHU0J_002291"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1216970.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11197..13098
                     /gene="mrdA"
                     /locus_tag="ACHU0J_002292"
     CDS             11197..13098
                     /gene="mrdA"
                     /locus_tag="ACHU0J_002292"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1216971.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13101..14213
                     /gene="mrdB"
                     /locus_tag="ACHU0J_002293"
                     /gene_synonym="rodA"
     CDS             13101..14213
                     /gene="mrdB"
                     /locus_tag="ACHU0J_002293"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1216972.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14224..15312
                     /gene="rlpA"
                     /locus_tag="ACHU0J_002294"
     CDS             14224..15312
                     /gene="rlpA"
                     /locus_tag="ACHU0J_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1216973.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15451..16662
                     /gene="dacA"
                     /locus_tag="ACHU0J_002295"
     CDS             15451..16662
                     /gene="dacA"
                     /locus_tag="ACHU0J_002295"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1216974.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16773..17036
                     /gene="ybeD"
                     /locus_tag="ACHU0J_002296"
     CDS             16773..17036
                     /gene="ybeD"
                     /locus_tag="ACHU0J_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1216975.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17137..17778
                     /gene="lipB"
                     /locus_tag="ACHU0J_002297"
     CDS             17137..17778
                     /gene="lipB"
                     /locus_tag="ACHU0J_002297"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1216976.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4555 a         4508 c         4554 g         4305 t
ORIGIN      
        1 ttcacacatg aataaaaaat taatgtatat attcgcaatt tttatagttg cagcaattac
       61 ctgtattagc caacccaaga aaacgatgtt gcgtgataaa gccatggtga attatgcctt
      121 tgattattta agatcaccag gcagtctacc gttaaccacg gcagccacgg agctttccgc
      181 gattcatggt cactcaacat cgcaatatcg ccttggcgaa ttttatcttc atggtagcga
      241 cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag cagaacagga
      301 aaatccgcgc gcacaaagca aactggggtg gatctacctg aaaggcctgg gagtcaaacc
      361 cgatacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt atgctcatgc
      421 tcaatatact ttgggtttga tctacagaaa tggcactggt attaatgtta accattatga
      481 atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg aaagattact
      541 tgccggactt cccgcacatt aacgtcgact gtcacccgaa gagatggagt attttcccta
      601 tcaaaccggc aaagataaaa atgtagaaaa taattttgat gacgcccaga cagcccagcc
      661 cgccatcatc aacgggcatt ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg
      721 taaaattcgc cgcctgaatt aataatcgac gaataattaa tgcttccagt ggacaacgat
      781 cttctgcatc aaggcggctc acaatcggca ttaatgattc ttctgtcggt tggctggcct
      841 gcactaattg tttggcaact aaatataatg gccataaggg atgttctgta tcacgcgtga
      901 gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagtgaa cgccccagcg
      961 cagagagttt ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc
     1021 cggggacata aagtagcggt tttccctgat cgttaaccag atcacgcgct tcaatcatca
     1081 tcaccactaa tgggtaataa tagacccgat gctgacggca ccattgcaac attaaagcat
     1141 ccgcctgggt ggagggatac tcgcgccagt agtaacaaag ttctgccagc aggctttcgc
     1201 cttctccaca atagacgcgt tgtgcgtatt cataataacg cgcatcttca ttatcaggtt
     1261 cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg gattcccagg cggcttgcgc
     1321 tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggataatc actgcgcgat
     1381 gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga gcgaacattt
     1441 ggtaaaaatc gatggtgtca ttttgcacgg caactggcag atgcagaatg gtctggaagt
     1501 taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct tcgtctattt
     1561 ccccgacgtt ttcctcctgc aaccaccgca atctttctgc taataacacc acgcactcga
     1621 aggataaatg ggcagtgttc atggcgattg tgcacagcga ccagcgtaat tcatcaatct
     1681 cttccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac
     1741 gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag aatttcatgt tctgctacct
     1801 catattcttc tggttgccaa acagatttag caggcgactg cgcaatccgt agcgcctcct
     1861 catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga aaggacggta
     1921 acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct atatcgagga
     1981 tcttccagca atttttcata agtcgctcat atccttaagc gtttccgggc atgtggggta
     2041 gtcagccagt ggttgccagg acaaggtagg gtaatacgca tcatcactgg caaataattg
     2101 ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt cgcgtaataa
     2161 aggcagatga tcatcttcgg gagactgcca atattgccga ccaaaggtat aaccggcaaa
     2221 gtattgccgc cagttactat aataacgtag agcccgtaga tgaatacgcg actggatcca
     2281 tagactttct tcttcactca accagttatt aagtacgccc attcgactta aaaaacccat
     2341 ccggacataa tcccatgctt taattcctcc ctcaccacag cattcggctg tgctggcgac
     2401 aaattgcgca taagcctgac cttgctcatt taattcagca agatagtcac gccattcaca
     2461 tggagaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat gaccgttctc
     2521 gatcattcgg tgaattatgc gcagcaggtc atcacggtca tcaattcccc atgaggaaga
     2581 aaggtcaaaa aagcatgtac ctaaatacat tttgggggat gaatgcgaag gtaaacagca
     2641 ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attttttgcg accccatgtc
     2701 catataattt atccttaaac attcatttaa tgaatgtaaa ctacatttcg aggctcagtt
     2761 atttatactt ttctgacgca acataatacg cataaaataa ggcttacgca aaaatggaac
     2821 acataaaaat ataatatatt ttatctacaa ccataaggaa atgataatga tttttacgtc
     2881 aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa aaggcgattg
     2941 cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg actctccgga
     3001 cgacgaaaag gccttttact ggctgaagct ggccgctgag caaggtcatt gtgaagcaca
     3061 gtattcctta gggcaaaagt ataccgagga taaaagccgt cataaagata atgagcaagc
     3121 catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca acgcccttgg
     3181 ctggatactg gatcgtggag aagaccccaa ctataaagaa gcggttacct ggtatcagat
     3241 agccgcggag agcggaatgt cttatgcgca aaataatctt ggatggatgt acagaaatgg
     3301 caacggagtc gcacaagact atacgctggc atttttctgg tacaaacaag ctgcattaca
     3361 aggccatagt tacgcacaag acaatctggc cgatctttat aaagacggag aaggcgttgc
     3421 tcaaaacaag acactcgccg cattctggta tttgaaaagc gcacagcagg gtaatcggca
     3481 cgcccagttt caaattgcat gggattataa cgctggcgaa ggggtggacc aggactataa
     3541 gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa
     3601 catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg ctgcctttga
     3661 atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc tggccattat
     3721 gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc tgtatcgtaa
     3781 agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg aagctttact
     3841 gtagaaataa aaacaagggc cggggaattt cattctgccc atattctctt tattgctgtg
     3901 cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcataa ccacatttcg
     3961 ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga
     4021 ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga ttgaggtcct
     4081 ggaaaatttc gcgccattca agctgcgttt tatcggtgat gtctgccagc tcctgccaca
     4141 agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg ctctcttcat
     4201 agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca tcgatctcgg
     4261 ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga
     4321 tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc gaccagttca cggtaatatt
     4381 gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat aatagacgtt
     4441 ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta cgaataatcc
     4501 gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg
     4561 ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga aaacaggacc
     4621 actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca
     4681 ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga agtattactg
     4741 cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac gtaactacac
     4801 catcggtgac gtgatcgccc gttaccagcg catgctgggc aaaaacgttc tgcagccgat
     4861 cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc
     4921 ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt
     4981 tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca
     5041 gaaattcttc accgagctgt ataaaaaagg cctggtatat aaaaagactt ctgcggtcaa
     5101 ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg gctgctgctg
     5161 gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca aaatcactgc
     5221 ttacgctgac gagctgctca acgatctgga taaactggat cactggccag acaccgttaa
     5281 aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa
     5341 cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac
     5401 ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga
     5461 actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg aaatggcgac
     5521 gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa cgggcgaaga
     5581 aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg cagttatggc
     5641 ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc tgaacatcaa
     5701 accggttatc ctggcggctg acggctctga gccagatctt tctcagcaag ccctgactga
     5761 aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt gaccatgaag cggccttcaa
     5821 cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct
     5881 gcgcgactgg ggtgtttctc gccagcgtta ctggggcgcg ccgattccga tggtgacgct
     5941 ggaagacggt accgtaatgc caaccccgga cgaccagctg ccggtgatcc tgccggaaga
     6001 tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg caaaaactac
     6061 cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta tggagtcctc
     6121 ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg attccgaagc
     6181 ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg ccattatgca
     6241 cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga
     6301 cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt
     6361 tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg aacgtgacga
     6421 gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt ataccggcat
     6481 gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg ttgaacgtta
     6541 cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata tgactctcga
     6601 atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt
     6661 gtacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga
     6721 tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct aaagtgaccg atgatatcgg
     6781 ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga acaaactggc
     6841 gaaagcaccg accgatggcg agcaggatcg cgctctgatg caggaagcac tgcaggccgt
     6901 tgtccgtatg ctcaacccgt tcaccccgca catctgcttc acgctgtggc aggaactcaa
     6961 aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag cgatggtgga
     7021 agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgtccc
     7081 ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa
     7141 atatcttgat ggcgttactg tacgtaaagt gatttacgta ccaggtaaac tcctcaatct
     7201 ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacactgtt gttatctctg
     7261 gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg acaccacgca ggttccttcc
     7321 actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag ccgtgcggtg
     7381 cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac
     7441 gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc ggtattccgt
     7501 aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt gttgatcccc
     7561 ggccgtgata tctacccgat tagcgccaaa gtcttccgtt cattcttcga taacccgcaa
     7621 atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta cgaccgtgct
     7681 gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg ttccgacgaa
     7741 gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc caccatgctg
     7801 ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat gaagggctgc
     7861 gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc caggacgctg
     7921 ttcgccaggt agctgcggca caaggattcg aagaacacca cactttttcc attgatccca
     7981 acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt gccagtcgac
     8041 aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc
     8101 tcacactcac cggacttctg catgacgacc tgctattgat cgtccgcggt aataaattaa
     8161 gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga
     8221 cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgtgcgc gcaaaacagc
     8281 tcaacttaga actggatgac gcggcgaatc aggtgctctg ctactgttat gaaggtaacc
     8341 tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac ggcaaactga
     8401 cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct tttcattggg
     8461 ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag caactgcgtc
     8521 tggaaggcag cgagccggtt attttgttgc gcacattaca acgtgaactg ttgttactgg
     8581 ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat aagcatcggg
     8641 tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagt cagccgcagt
     8701 tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa gattacggtc
     8761 agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa cccctggcgg
     8821 acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg cacctttgat
     8881 ccggtgcact atggtcatct aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg
     8941 cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga agcgaacagc
     9001 gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt tactcttgat
     9061 gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa agagtggcgg
     9121 caggaacaag gaccggacgt gccgctggcg tttattattg gtcaggattc attgctgacc
     9181 tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg
     9241 cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct ggaagatcat
     9301 ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta tctggctgaa
     9361 acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg
     9421 tgtgaggatt tattgccgga accggtattg acttacatta accaacaagg cttgtatcgc
     9481 tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat ggcgaaacac
     9541 aagcgaatgt cgatggtctg tacagcggtc atgcgcccac acccctgacc gcacgcggta
     9601 ttgagcaagc gcaaaatctg catacactgc tagatgatgt ttcctttgac ttggttttat
     9661 gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac cgccagctcc
     9721 ccgttcatat catccctgaa ctcaacgaaa tgttttttgg cgactgggag atgcgacatc
     9781 atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat gactggcagc
     9841 atgctatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg gaacgcttta
     9901 tcgcaaggct tagtgaatat cagcactatc agaatatttt aatcgtcagc catcagggcg
     9961 tactgagtct gttaattgct cgtttaattg gcatgcctgc cgagtctatg tggcattttc
    10021 gcgttgacca ggggtgttgg agcgccattg atatcaacca aaaattcgcg acgctacgcg
    10081 tcctcaatag ccgtgccatc ggggtcgaga acgcatgact tttctgtttt tttacgggta
    10141 agccgcaacg accattgaca gccccgggca ggctgatatt ctccgcagcc agactttttc
    10201 cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg
    10261 ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg aaatcgcctc
    10321 tggttcaggt atactgacag accattttta tctatttgat tcacccaggg ggaaaacttg
    10381 cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa aggtcaggac
    10441 atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat catctgtacg
    10501 ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca
    10561 gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat
    10621 ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa
    10681 aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac
    10741 tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat gcccttcgag
    10801 ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg acatcaagcg catactcgac
    10861 aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt
    10921 ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg gaagctggat
    10981 ggtcgcgacg taagtctact gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg
    11041 gcggctgagc agagctggtc gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg
    11101 ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt
    11161 gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa ctcttttcgc
    11221 gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt
    11281 ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt tcgctttacc
    11341 gactaccaga cccgctctaa tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc
    11401 ggcattatct acgaccgtaa cggtatccct ctggccctca accgcactat ctaccagata
    11461 gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg
    11521 gtagatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt
    11581 ttcacctcta ttccggtgaa aactaaccta accgaagtac aagtagctcg ctttgccgtc
    11641 aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta ctatccttac
    11701 ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa
    11761 cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat tggtaagctg
    11821 ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga agaggttgaa
    11881 gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca agccggacac
    11941 gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct gctggcgggt
    12001 agccgcgcag ctgtggttgt taccgaaccg cgtacaggtg gggtgctggc gctggtttcc
    12061 acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga ttattccgcc
    12121 ttgctgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt ttatcctccc
    12181 gcgtctacag ttaaacccta tgtggcggtt tcggcattaa gcgccggggt gatcacgcgc
    12241 aatacgacgc tgtttgaccc aggctggtgg caactaccag ggtcggaaaa acgttatcgt
    12301 gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg
    12361 gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc cgaatggatg
    12421 ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg ttccggcaac
    12481 atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca gggtgacacc
    12541 attccggttg gtatcggcca gggttactgg acagcgaccc caatccagat gagtaaggca
    12601 ctgatgatcc tgattaatga cggtatcgtg aaggttccgc atttgctgat gagcaccgcc
    12661 gaagacggca aacaggtgcc atgggtacaa ccgcatgaac cgcccgtcgg cgatattcat
    12721 tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg ccctaatggt
    12781 acggcgaata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc tggtaccgct
    12841 caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc cgagcgttta
    12901 cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc
    12961 atgattctgg agaacggcgg cgcgggtccg gcggttggta cgctgatgcg ccagatcctc
    13021 gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc agcggttgcc
    13081 gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc tgggataaag
    13141 tccatctcga tcccacaatg ctgctgattt tactggcatt gctggtttac agcgccctgg
    13201 ttatctggag cgccagcggt caggatattg gcatgatgga gcgtaaaatc ggccaaatcg
    13261 cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct
    13321 gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat gctttcggag
    13381 ccatctctaa aggcgctcaa cgctggctgg acctcggtat tgttcgtttt cagccgtcgg
    13441 aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc
    13501 cgccatcgtt gaagaatact ggcatcgcgc tggtgctgat atttatgccc acgctgctgg
    13561 tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac
    13621 tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg gtagcggcgt
    13681 tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc gtaatgatgc
    13741 tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag tctaaaattg
    13801 ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag tcacagcttg
    13861 aatttctccc cgaacgccat actgacttta tcttcgcggt actggcggaa gagctgggat
    13921 tagtgggcat tctgattctg ctcgctctct acattctgct gatcatgcgc gggctgtgga
    13981 tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta atgctgatat
    14041 tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg gttgtagggg
    14101 ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg gctgggttcg
    14161 ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg taagaggtgc
    14221 gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca
    14281 agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg taacggcccc
    14341 atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag
    14401 gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg atttagccag
    14461 gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc ctctggcgaa
    14521 gctttcgatc cgacaaagct gacggcggcc catccaacgc ttccgatccc cagctacgcc
    14581 agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga tcgcggccct
    14641 tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct taacacgtca
    14701 aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc gctttctggt
    14761 cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgcctgc acctcccgat
    14821 ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat tcttccggtc
    14881 agtaattcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag tagcggtttc
    14941 ctcggcgctc caacgacctt agcgcctggt gtactggaag gcagcgaacc gacgcctgcg
    15001 ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa cctcgcctgc aatggtgaca
    15061 ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc
    15121 gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg cgtccccggt
    15181 cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gcccattcgc cagcaaagcc
    15241 gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc atttattact
    15301 accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa
    15361 agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg gcactttttt
    15421 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    15481 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    15541 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    15601 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    15661 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    15721 aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac cggtaacccg
    15781 gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    15841 ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    15901 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgcactgggc
    15961 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    16021 gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa tgaatactcg
    16081 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg
    16141 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    16201 tacaatcttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggt
    16261 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt
    16321 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    16381 tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattccg
    16441 cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    16501 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc
    16561 gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc
    16621 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag
    16681 tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt ctggcgggag
    16741 ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac gaactgcttg
    16801 aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag ctggttgatc
    16861 aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg gtaaaaccaa
    16921 gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat atcgagcagg
    16981 ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt ctgtaactcg
    17041 cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg atatactttt
    17101 cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa aattcttgtc
    17161 cgccagctcg gtcttcagcc ttacgagcca atctcccggg ctatgcatga attcaccgat
    17221 acccgcgata atagtaccct tgatgaaatc tggctggtcg agcactatcc ggtattcacc
    17281 caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc ggtgatccag
    17341 agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat gtatgtgttg
    17401 cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca
    17461 gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga cgcgcctggt
    17521 gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg cggttgttca
    17581 ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg tattaatcct
    17641 tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga agcgacgact
    17701 aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa tccggacttc
    17761 gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg tcattactca
    17821 taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta tcacttgcag
    17881 aaatgcggtg ccacgccttg ctccgtaact caccccattg at
//
LOCUS       DAZPDE010000077        17909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPDE010000077 DAZPDE010000000
VERSION     DAZPDE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.077"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            97..621
                     /locus_tag="ACHU0J_002298"
     CDS             97..621
                     /locus_tag="ACHU0J_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR1216977.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            702..1376
                     /locus_tag="ACHU0J_002299"
     CDS             702..1376
                     /locus_tag="ACHU0J_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1216978.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1392..3854
                     /locus_tag="ACHU0J_002300"
     CDS             1392..3854
                     /locus_tag="ACHU0J_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1216979.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3870..4903
                     /locus_tag="ACHU0J_002301"
                     /pseudo
     CDS             3870..4903
                     /locus_tag="ACHU0J_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4985..5323)
                     /gene="rcnB"
                     /locus_tag="ACHU0J_002302"
     CDS             complement(4985..5323)
                     /gene="rcnB"
                     /locus_tag="ACHU0J_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1216980.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5542..6372)
                     /gene="rcnA"
                     /locus_tag="ACHU0J_002303"
                     /pseudo
     CDS             complement(5542..6372)
                     /gene="rcnA"
                     /locus_tag="ACHU0J_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6494..6766
                     /gene="rcnR"
                     /locus_tag="ACHU0J_002304"
     CDS             6494..6766
                     /gene="rcnR"
                     /locus_tag="ACHU0J_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1216981.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6858..6954
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6989..7777
                     /gene="thiM"
                     /locus_tag="ACHU0J_002305"
     CDS             6989..7777
                     /gene="thiM"
                     /locus_tag="ACHU0J_002305"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1216982.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7774..8574
                     /gene="thiD"
                     /locus_tag="ACHU0J_002306"
     CDS             7774..8574
                     /gene="thiD"
                     /locus_tag="ACHU0J_002306"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1216983.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8639..9456
                     /locus_tag="ACHU0J_002307"
                     /pseudo
     CDS             8639..9456
                     /locus_tag="ACHU0J_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9508..10254
                     /locus_tag="ACHU0J_002308"
     CDS             9508..10254
                     /locus_tag="ACHU0J_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1216984.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10228..11193)
                     /locus_tag="ACHU0J_002309"
     CDS             complement(10228..11193)
                     /locus_tag="ACHU0J_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1216985.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11190..12193)
                     /locus_tag="ACHU0J_002310"
                     /pseudo
     CDS             complement(11190..12193)
                     /locus_tag="ACHU0J_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12233..13285
                     /gene="fbaB"
                     /locus_tag="ACHU0J_002311"
     CDS             12233..13285
                     /gene="fbaB"
                     /locus_tag="ACHU0J_002311"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1216986.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13593..14447
                     /gene="gatY"
                     /locus_tag="ACHU0J_002312"
     CDS             13593..14447
                     /gene="gatY"
                     /locus_tag="ACHU0J_002312"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1216987.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14476..15738
                     /gene="gatZ"
                     /locus_tag="ACHU0J_002313"
     CDS             14476..15738
                     /gene="gatZ"
                     /locus_tag="ACHU0J_002313"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR1216988.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15748..16200
                     /gene="gatA"
                     /locus_tag="ACHU0J_002314"
     CDS             15748..16200
                     /gene="gatA"
                     /locus_tag="ACHU0J_002314"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1216989.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16231..16515
                     /gene="gatB"
                     /locus_tag="ACHU0J_002315"
     CDS             16231..16515
                     /gene="gatB"
                     /locus_tag="ACHU0J_002315"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1216990.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16519..17874
                     /locus_tag="ACHU0J_002316"
     CDS             16519..17874
                     /locus_tag="ACHU0J_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1216991.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4666 a         4098 c         4558 g         4587 t
ORIGIN      
        1 tgtttacatt gcggcagctt ttttgatgtg agaaatttgt ttccaagaga tacagactct
       61 taacaaccaa aaataaattt tattaaggaa catattatga aacgttcaat tattgctgcc
      121 gctgtctttt cttctttttt tatgagcgct ggagtatttg ctgcagacgt tgataccgga
      181 acattaacca ttaaaggaaa tattgcagaa tctccgtgta aattcgaagc gggtggtgat
      241 tcagtaagta ttaatatgcc gactgtacca accactgtct ttgaaggtaa agctaaatat
      301 tctacctatg atgatgcagt cggtgtaacc agcagcatgt taaaaattag ctgcccgaaa
      361 gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca aaataaccgg taacgataag
      421 gcgatagcca gtagcaacga taccgtaggt gataacagcg atgtcctaga tgtttctgca
      481 ccttttaaca ttgagagtta taaaacagcg gaaggtcaat atgctattcc gtttaaagca
      541 aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc ttctctggtt
      601 atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt ctccaggtaa taaacatggc
      661 cgctatcccc atggcggctt tttaatttaa gaggcattaa gatgaaagga ttattatctt
      721 tactcatttt ttctatggtc cttcctgcac atgccggaat tgttatctac gggacgcgca
      781 ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag ggaaatcgtt
      841 cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc gttaccacca gaaaaaattc
      901 aggttccttt catgttaacg ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag
      961 taaaaatcaa aattatgccg aataaactgc ccactaataa agaaagcatt ttttatctga
     1021 atgttctgta tattccacca aatagtccag agcaagaagg caagaatgca ctgaagtttg
     1081 cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg gtaaataaag
     1141 cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata aaaaatgact
     1201 cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc aattatgaaa
     1261 ctattatgat tgccccctta gaaagtcaga gtgttaatat caaaagtaat aatgcaaata
     1321 actggtatct gaccattatc aatgaccacg gcaactatat tagtgacaaa atttaattgc
     1381 aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg ttattgctcg
     1441 gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata ggtggaatga
     1501 aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg gggcagtatg
     1561 acatcgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt gttaaagaca
     1621 acccgcaaga aacatgttta tcaagagaag ttatcaagcg gttaggcatt aatagcgata
     1681 acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt gggagctata
     1741 gctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcttgg gtggaagaac
     1801 tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg ttttatactt
     1861 cttattatgt gagtcagtat tacagcgact ataaagcgtc gggtaacaac aagagtacat
     1921 atgtacgttt taacagcggg ttaaatttac tggagtggca actgcattct gatgccagtt
     1981 tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg
     2041 gatttgccca atttctcggc acgcttcgcg tgggtgatat gtacacatca agcgatattt
     2101 ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact
     2161 cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaacgcg ctggtaacta
     2221 ttgaacagaa tggttttgtg gtttatcaga aagaggttcc tcctggcccg tttgcgatta
     2281 cagatttgca gttggccggt ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg
     2341 gctcggtaac cacctatctg gtgccttatg cagcggtgcc aaatatgctg caacccggcg
     2401 tgtcgaaata tgattttgcg gcgggggcga gcaagcaaag tgattttgtc caggcaggtt
     2461 atcagtatgg ttttaataac ttattgacgc tgtatggcgg cacgatggtt gctaataatt
     2521 actatgcgtt taccctcgga acgggttgga acacgcgcat tggcgcaatt tccgtcgatg
     2581 ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa
     2641 ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt
     2701 attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat aaagataatt
     2761 atcgccgtga tgaaaacgat atctatgaca ttgccgatta ttaccagaac gattttggcc
     2821 gtaaaaatag cttttctgcc aatatgagcc agtcattgcc agaaggctgg ggttctgtgt
     2881 ccttaagtac gttatggcga gattactggg ggcgtagcgg cagcagtaag gattatcagt
     2941 tgagttattc caataactgg cggcggataa gctataccct cgcggcaagc caggcgtatg
     3001 gcgagaatca tcatgaagag aaacgtttta atatttttat atcaattccc tgtgattggg
     3061 gtgatgacgt tacgacgcct cgtcggcaaa tatatatgtc taactcaacg acgtttgatg
     3121 atcagggttt tgcctcaaat aatacgggat tatcaggaac agtagggagt cgggatcagt
     3181 tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca gctggggcga
     3241 atttgacctg gaacgcgccg gttgcgacag tgaatggcag ttatagtcag tcgagtactt
     3301 atcgacagac tggagccagt gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc
     3361 tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt aaagatgctt
     3421 atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta tacgacggaa
     3481 tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag
     3541 aattacgtgg caaccggaaa attgccgccc cttatcgcgg cgcggttgta ctggttaatt
     3601 ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat ggtcaaccat
     3661 taacgtttgg ttatgaagtc aatgatatcc atggtcataa tattggcgtt gtcggccagg
     3721 gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta gcaattgata
     3781 agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa agtagaaatt
     3841 atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta tttatattaa
     3901 tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct gttcagcaca
     3961 atttggttgt tcaggggaat tttgcactta ctcaaacaca aatagcgaca tatgagcata
     4021 attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagc ccatcggata
     4081 ttattgttgg actttataac gataccatta aattaaattt acattttgaa tggaccaata
     4141 aaaacaacat cacgttgtca aataatcaga gcagtttcac cagtggttat tcggtaacgg
     4201 tgacacctgc ggccagtaat gcaaaagtga atatttctgc ggggggcggt ggttcagtga
     4261 tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg agtgccgcag
     4321 tacaatttct actgtgttta ttaggtggca agtcatgggg tgcatgtgta aatagctaca
     4381 gaaatgcatt ggcacaaaat gcaggtgtct attccttcaa tctgacattg tcatacaacc
     4441 cgataaccac aacctgcaaa caggacgatt tattaattac tttagacagt attcccgttt
     4501 tacaattacc agccacaggt aacaaagcaa caataaatag taaaaaaagg gatattattc
     4561 tgcgttgtaa aaatttatta ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt
     4621 tatcaagttc tgacttgtta accaacagca acacaatact gaaaggtgcg gaagataatg
     4681 cgtaggattt attcttgaaa gtaatggttc gccagtcaca cttttaaata tcactaacag
     4741 cagtaaagga tacacaaatt taaaagaaat tgcggcaaag tcaaaactta cagatacaac
     4801 ggtttcaatt ccgataacag ccagttacta cgtctacgat acaaacaaag ttaaatctgg
     4861 cgcactggag gcaaccgcat taatcaacgt gaaatacgac taataacaag caaaacgagc
     4921 aagtggctaa taataaaaaa taacaaggtg tctgcatttc cccctcatga tgagggggct
     4981 ttttttagcg atgataaaaa atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt
     5041 cgctgatcaa cacgtacgcg ccacccatat aggtccagtg cgtcccggca tcaggcgcgg
     5101 gcaggttacg taactgccac tgcttaatgt tgtactcatc ggtgcggtac atctctggga
     5161 cggtatcacc tggtttgaaa tgcttaaagt cagcaatgaa actacttaac tcatacttag
     5221 aaattcccga ggtattggtc gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa
     5281 ccagcaaaag cacacccagc aacatcttat ttttaatggt cataaactct ccactttggg
     5341 gatctgtaat atcagtcgca tatctttcct gtcatacaat ataactgaca agcgatattt
     5401 tgttagtgat cccatgattt cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct
     5461 tagcgagcag agagtcagcg gaaatagctg atttaagaat catcctgggc tgaagataac
     5521 atgcaagcct aaaggattga gttatcgcat tatgcccatg aagccatgta cgcccatata
     5581 cacaccgact aagccaatca acagactgga aaaatagggg gcgcgtttag cgagagtgtt
     5641 aaatccgctc cagcgttttg cgacctgctg aacgctgatt gctgcgccaa cgcctacggt
     5701 gacaagcgtt aacgccaggc caatgctgaa actgacgacc agtgttgcgc ccagtgtcag
     5761 ggctttcaac tgaatgcata tcaacagcac ggtaattgct gccgggcagg ggataaagcc
     5821 accggttaag ccaaataaca aaatttgcca gttgatgacc tctctaccat caaagcgtcg
     5881 tttaatgtca ttggcatggg ctcgtgcatg ggcatcctga tactcgccat gttcgtgatg
     5941 gtgatgatgt ccttggtcgt ggtgatgttc gtgatcgtga tgatggtgat gatgctcata
     6001 atcatgcccg tgcatattct ccagccagtt gcgttcgccg cgctaggtac gccagaacat
     6061 ccagaacgcg gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga
     6121 ttgagcagta aagcgcttac tgatcaccat cccgccaaag gcaatcaacc agaccaccgc
     6181 ggtatgcgaa atggttgctg ccagtccgag catcaccgcc tgtttaatgg tgcctttgat
     6241 ggcgatgata aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc
     6301 accaagtaag atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt
     6361 aaattcggtc atgataataa ttcttagtat taattcggca atctgattct actccccccc
     6421 agtacctgat actacccccc cagtagatta atagtgctat gatttttcat attcttgtta
     6481 accaggtgtt gccatgtctc atacaatccg tgataaacag aaactgaaag cgcgtgccag
     6541 taagattcag ggccaggtcg tggcgctcaa gaaaatgctc gacgagccgc acgaatgcgc
     6601 tgcagtttta caacagattg ctgctatccg tggagcggta aacggtctga tgcgggaagt
     6661 gattaaaggt catctgacgg aacacatcgt tcaccagggg gatgagctaa aacgtgaaga
     6721 agatctggat gtcgttctga aggtgctgga ttcatatatc aaataattta ttaacgcgat
     6781 tgtaaaactg ccgtttttcc tcgtttacaa cgggggcgct ggacattacc atcctcctct
     6841 gcgatttatc atcgcaacca aacgactcgg ggtgcccttc tgcgtgaagg ctgagaaata
     6901 cccgtatcac ctgatctgga taatgccagc gtagggaagt cacggaccac caggtcattg
     6961 cttcttcacg ttatggcagg agcaaactat gcaagtcgac ctgctgagtt cagcgcaatc
     7021 tgcgcacgcg ttacaccttt ttcaccaaca ttcccctctt gtgcactgca tgaccaatga
     7081 tgtggtgcaa acctttaccg ccaatacctt gctggcgctc ggtgcatcgc cagcgatggt
     7141 tatcgaaacc gaagaggcca gtcagtttgc ggctatcgcc agtgccttgt tgattaacgt
     7201 tggcacactg acgcagccac gcgcacaggc gatgcgtgct gccgttgagc aagcaaaaag
     7261 ctctcaaaca cgctggacgc ttgatccagt agcggttggt gcgctcgatt atcgccgcca
     7321 tttttgtcat gaacttttat cttttaaacc ggcagcgata cgtggtaatg cttcggaaat
     7381 catggcatta gctggcattg ctaatggcgg acggggagtg gataccactg acgccgcagc
     7441 taacgcgata cccgctgcac aaacactggc acgggaaact ggcgcaatcg tcgtggtcac
     7501 tggcgaggtg gattatgtta ccgatggaca tcgtatcatt ggtattcacg gtggcgatcc
     7561 gttaatgacc aaagtggtag gaactggctg tgcattatcg gcggttgtcg ctgcctgctg
     7621 tgcgttacca ggcgatacgc tggaaaatgt cgcatctgcc tgtcactgga tgaaacaagc
     7681 cggagaacgc gcagtcgcca gaagcgaggg gccaggcagt tttgttccac atttccttga
     7741 tgcgctctgg caattgacgc aggaggtgca ggcatgaaac gaattaacgc tctgacgatt
     7801 gccggtactg atccgagtgg tggtgcgggg attcaggccg atcttaaaac cttctcggca
     7861 cttggcgctt atggttgctc agttattact gcactggtgg cgcaaaatac ccgtggcgta
     7921 cagtcggtgt atcgcattga gcctgatttt gtcgccgccc agctcgattc ggtgttcagc
     7981 gatgtgcgaa tcgataccac taaaatcggt atgttggcgg aaaccgatat tgttgaagcg
     8041 gtggcagaac ggttgcaacg ttatcagatc caaaacgtgg tactcgacac cgttatgctg
     8101 gcaaaaagcg gcgacccgct gctttcacct tcggcggttg ctacgctgcg cagtcgatta
     8161 ttgccacagg tttcattaat aacgccaaac ttgcccgaag ctgccgcctt gctcgacgcg
     8221 ccacacgcgc gcaccgaaca ggaaatgctg gaacaagggc gatcgctgtt ggcgatgggc
     8281 tgtggcgcag tgctaatgaa aggtggtcat ctggatgatg agcaaagccc ggactggctg
     8341 tttacccgcg agggtgaaca acggtttacc gcaccgcgca ttatgaccaa aaacacccac
     8401 ggcactggtt gtacactctc tgcggcgttg gctgcactac gcccgcgcca tacaaactgg
     8461 gctgacaccg tacaggaggc aaaaatctgg ctttcatcgg cgttagccca ggccgacacg
     8521 ctggaagttg gtcacggtat tggtccggtt caccacttcc acgcctggtg gtgagcaccg
     8581 tactatagct gctttggtta ttgtcagcct ttggcactga cattcaggga tgaaaccaat
     8641 gcagctaaga attaccagtc gaaaaaaact caccgctcta ttatgcgctc ttgggctaat
     8701 ttccatcgtt gcaatttatc cgcgtcagac ggtgaatttt ttctattcga cagcagttca
     8761 gattacagac tacatccact tctacggtta tcgcccggtt aaatctttcg ctattcgcat
     8821 tcctgccagt tacaccattc acggattaga tgtttcgcgc tggcaggagc gtatcgactg
     8881 gcagcgggtg gcaaaaatgc gcgacaacga tatccgctta cagtttgctt ttattaaggc
     8941 tacggaaggc gaaaagctgg tggaccccta tttttcgcgt aactggcaat taagccgcga
     9001 aaatggcctg ctgcgcgggg cgtatcatta tttctccccg tcggtatccg cttcagttca
     9061 ggcgcgatta tttctgcaaa cggtggattt ctcacaaggc gatttccctg ccgtgctgga
     9121 cgtagaagaa cggggaaaat tatcggcaaa agaattacgc aagcgggtaa gtcagtggct
     9181 aaaaatggtc gaaaaacgca cgggaaaaaa gccgattatt tactcaggag ccgtttttta
     9241 tcacaccaat ctggcgggct atttcaatga atatccgtgg tgggtggctc actattatca
     9301 acgtcgtccg gacaatgacg gcatggcctg gcgcttctgg cagcattccg accgtggaca
     9361 ggtagatggc atcaatggtc cggtggattt taatgtgttt aatggcacgg ggatgagttg
     9421 caggcattcg ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca
     9481 ccagactccc tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa
     9541 ctgaacgagc gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg
     9601 gtaaaaaatg ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt
     9661 gatttaagtc agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg
     9721 gaagaagagg gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc
     9781 gaatactcgt tgaaagaagc gagaggtttt tctcagcagg tggtattgcg cgggaaaaag
     9841 cccgatacgc tatgggttaa taagcgtgtc gtgaaatgtc ccgaagaagt tgcgcagcag
     9901 ctggcggccg aagcgggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa
     9961 gaagcggtat cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc
    10021 atcggaattt ctctttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt
    10081 tcccgcgtta gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc
    10141 aaaataccgg tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag
    10201 tacagcatta gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc
    10261 gcgcgttggc gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc
    10321 cagtaatacg gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc
    10381 atgactgtcg cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc
    10441 agaagcgtcg ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc
    10501 ggcaaatttc tcctgccatt gcttgccaag tgttgttatc tctgcggata aagcaaaacg
    10561 ttcggcggca atctcagcct cttgacgatt gagtgacact aaaggtcgac aggccatgat
    10621 ccgcgccagt aatgcgtccg ggatatcgcc aatacgtggg caaaaatcga taaacggcgt
    10681 cacgtcttgc agcttttcca gccattccac taacaattcg ccgcagggcg aggccagttg
    10741 ataaccggaa aaatagagca ggttgccagg cgcaacggtt aatcgtgcca accattggcg
    10801 attccattga ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac
    10861 cagtgccaga caccagccgt tatcgctttc ggcgttatcg atcaggctga ttaacccctc
    10921 tttcgccatc cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt
    10981 acccgcttcg atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac
    11041 gttaacgctc tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc
    11101 ggcgatcacg tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc
    11161 aggcagcagt gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc
    11221 gccagcgctg tggcatagcg gttgaaatca agctgattta ccgcatccag ctccgccttt
    11281 aacgcaggat cgacagcgtt aatgccatgc aacgcgccgc aaatcgccgt cgccatagca
    11341 cctatggtgt ctgtgtcgcc gccaagattg gcgcacagga cggcgcagcg gtttgggtcg
    11401 gtttgtgcca gttcaactag cgcaatggcg cacggaacgg actcaatagt gctggtgcct
    11461 gcgccaacga cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attacgcaca
    11521 attttcagcg caatctccag acgtgccgcc agtgaggcgc tgaacgtggt ggtgcgtttt
    11581 tgctgtgcat gtcgcgcaat tgaaggcaat gaatcaacaa tcgctgacca gcgttctccg
    11641 tcaatggcac gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat
    11701 ttatgggtcg ggctggacgc cagcgccaca tcatcaataa aggaatcaac atcacgcgct
    11761 ggaagcaaac aacctaatgg cgagacgcgc atcgctgcgc cgttggttac gccgttgttt
    11821 tccagttcag caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga
    11881 cctagtacgt ttttgttaaa ggcgtcgaaa cgcaacgccc agtcgagaat attacgccca
    11941 atcagattcg gatcgatctt gccttcacat tccagtaacg catccgccag acacaggcca
    12001 tcgaggtatc gtcggtgaat tcggcgcggt taaaataaca ggccgcgtta ttctcctttg
    12061 gtccaggcag aaaacggtca atccagccaa agtgtgcttt aacgcggctg cgtggccaaa
    12121 gctcggaggg catccccatc gcatccccta acgcctgccc ataaagagca ccgagaatac
    12181 gttctgtttt catttaactt ccccttgaat cggaatttac gggagcatag taatgacaga
    12241 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgactat
    12301 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtga tgattgacaa
    12361 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    12421 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    12481 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    12541 agcgggctgt aactgtgtgg catcaactta cggcgtgttg gtgtcggtat cgcggcgcta
    12601 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    12661 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    12721 ggttggtgcg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    12781 tgcggccttt gaacgcgcgc acgagctggg catggtgaca gtgctgtggg cctatttgcg
    12841 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgactggtca
    12901 ggcgaaccat ctggcggcaa ccataggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    12961 taacggcggc tataaagcga ttaattacgg ttataccgac gatcgcgtgt acagcaagtt
    13021 aaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    13081 ggctgggttg ataaactccg gcggtgctgc aggcggtgaa actgacctca gcgatgcagt
    13141 gcgtactgcg gttatcaaca aacgcgcagg cggaatgggg ctgattcttg gacgtaaggc
    13201 gttcaagaaa tcgatggctg acggcgtgaa actgattaat gccgtgcagg acgtttatct
    13261 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13321 gataagacgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    13381 catgcctaca tagcactgcc acgtatgttt acaccgcatc cggcataaaa acacgcgcac
    13441 tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc gaacaatttt
    13501 catattgttg tttttgcgat cattatctcg acatttaaaa gcaaataatt tcattatatt
    13561 ttgaaatcga aagcaaacga caagatatga aaatgtacgt ggtatcgaca aagcagatgc
    13621 tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac aatctcgaaa
    13681 cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc atcgccggaa
    13741 cgcctggcac atttactcat gctggtacag aaaatctgtt ggcgctggtc agcgcgatgg
    13801 cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa tttgacgata
    13861 tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt
    13921 ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc tttgatgtca
    13981 gcgtcgaggc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag
    14041 tcgatgcgtt gtacaccaac cccgcacagg cgcgtgaatt tgccgaggca accgggattg
    14101 attccctggc ggtcgccatc ggcacggctc acggtatgta tgccagcgca ccggtgcttg
    14161 atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg ctgcatggcg
    14221 cgtcaggatt atcgactaag gatattcagc aaaccatcaa actggggata tgcaaaatca
    14281 acgttgcaac ggagctgaaa aatgccttct tgcagtcgtt aaaaaattac ctgaccgagc
    14341 accccgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca atgcgcgatg
    14401 tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc acctgccatt
    14461 taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga acatatcggc
    14521 atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc atttgatcgc
    14581 aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca atttggcggt
    14641 tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc cgataaagtt
    14701 gggtttgcac gcgaacgcat tattctcggc ggcgatcatc tgggaccaaa ctgctggcag
    14761 caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt
    14821 gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga taccataccg
    14881 ttagcaccag aaacggttgc ggaacgagct gctgtgcttt gctttgctgc ggaaagtgtg
    14941 gcgacagatt gccagcgtga gcaactgagc tatgtcattg gcactgaagt tccggttccg
    15001 ggcggtgagg ccagcgccat tcagtcagta cacatcaccc atgttgaaga tgccgccaat
    15061 actttacgta cgcatcaaaa ggcctttatt gcccgtgggc tgacagaggc gttaacacgt
    15121 gtgattgcca tcgtggtgca gccgggtgtg gaatttgatc acagcaatat tatccattat
    15181 cagccgcagg aagcgcaggc gctggcgcaa tggatagaaa acacccgaat ggtttatgaa
    15241 gcacattcta ccgattacca gacccggacg gcttattggg aattagtccg cgatcacttt
    15301 gcaatattga aagtcggtcc cgcattaacc tttgctttac gcgaggcgat atttgcactg
    15361 gcacaaattg agcaggaact tatcgccccc gaaaatcgca gcggttgcct ggcggtaatt
    15421 gaagaagtga tgctcgacga accgcaatac tggaaaaaat attatcgcac gggttttaac
    15481 gattcattac tggatattcg ttacagcctg tcggatcgta ttcgttatta ctggccgcat
    15541 agtcggatta aaaatagcgt tgaaacgatg atggtgaatc tgcaaggcgt ggacatccca
    15601 ctgggcatga ttagtcagta tcttcccaaa caatttgaac gcattcagtc cggggaatta
    15661 tcagcaatgc cgcatcagct tattatgaat aaaatttatg atgttttgcg cgcctatcgc
    15721 tacggctgtg cggaataagg acggtatatg actaacctgt ttgttcgtag cggaatttct
    15781 tttgtcgatc gtagcgaagt tttaacctat atcggtaatg agatgctcgc gaaaggtgtg
    15841 gttcatgata cctggccaca ggcattaatt gccagagaag cagaattccc taccgggata
    15901 atgcttgagc agcacgccat tgcaataccg cattgtgagg cgattcatgc taagtcgtca
    15961 gccatttatc tgttaaggcc aacaaataaa gttcattttc agcaagcgga tgatgataac
    16021 gacgtggcgg tatcgttggt tattgcgttg attgtggaaa atccgcagca gcaattgaaa
    16081 cttttacgct gtttatttgg caagttacaa cagcccgata tcgtcgagac actaattact
    16141 cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg ttttagattc agacgaataa
    16201 atccctctgt aacaataaaa aggactattt atgaaacgca agattattgt cgcttgcgga
    16261 ggcgcggttg cgacctctac gatggcggcg gaagaaatta aagagttgtg tcagagtcat
    16321 aatattcctg ttgaattaat ccagtgtcgg gttaatgaaa tagaaaccta tatggatggt
    16381 gtgcatttga tatgtaccac tgccagggtg gatcgtagtt ttggcgatat tccgttagtt
    16441 cacggcatgc cttttgtttc tggtgtcggt atcgaagcat tacaaaataa aattctgact
    16501 atcttacagg gatgacctat gttttcagaa gtcatgcgtt atattctcga cctcggccct
    16561 acagtgatgc tgccggttgt catcattatt ttttctaaaa tattaggcat gaaggcaggc
    16621 gattgcttta aagcgggtct gcatatcggg attggctttg ttggcattgg ccttgtgatt
    16681 ggcttaatgc tggattccat tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg
    16741 aatctgcatg tggtcgatgt cggctggccg ggctcttcac caatgacctg ggcgtcgcaa
    16801 attgcgctgg tggcgattcc gattgcgata ttggttaacg tggcgatgtt actgacccgt
    16861 atgacgcggg tggtaaatgt tgatatctgg aatatctggc atatgacctt caccggcgcg
    16921 ttgctgcacc tggcaaccgg ttcatggatg atagggatgg cgggtgtggt aattcacgcg
    16981 gcgtttgttt ataagctcgg cgactggttt gcccgcgata cccgaaattt ctttgagctg
    17041 gaaggtattg ctattccgca cggtacgtcg gcgtatatgg ggccgattgc ggtgctggtc
    17101 gatgctatca tcgagaaaat cccaggcgtt aaccgaatta aatttagcgc cgacgatatt
    17161 cagcgcaaat ttggcccttt tggcgaacct gtcaccgtgg gttttgtgat ggggctgatt
    17221 atcggcatcc tcgcgggtta cgatgtcaaa ggtgtattgc agctggcggt aaaaacggcg
    17281 gcagttatgc tgctgatgcc acggatgatt aaacccatca tggatggttt aacgcccatc
    17341 gctaagcagg cccgtagtcg tttacaggcg aagttcggcg gtcaggagtt cctgattggc
    17401 ctggatcccg cattactgct ggggcatacg gcggtggtat cggcaagcct gatttttatt
    17461 ccgctcacca ttttaattgc tgtttgtgtt ccgggtaatc aggtactgcc gtttggcgat
    17521 cttgccacta tcggcttctt cgtggcgatg gcggtcgcgg tgcatcgtgg aaatctgttc
    17581 cgcaccttaa tctcgggtgt catcattatg agcatcaccc tgtggatcgc gacgcaaact
    17641 attggtttgc acacccaacc ggcggctaat gctggggcgt taaaagccgg gggtatggtg
    17701 gcttcaatgg atcagggcgg ttctcccatt acctggttac tgattcaggt tttctccccg
    17761 caaaatattc ccggtttcat tattatcggc gcaatttatc tgaccggtat tttcatgacc
    17821 tggcgtagag cgcgtggttt tactaaacaa gagaaagccg ttctcgcaga ataattttta
    17881 cctgaggggg aggttatccc ccttaacaa
//
LOCUS       DAZPDE010000078        17758 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPDE010000078 DAZPDE010000000
VERSION     DAZPDE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.078"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(88..1371)
                     /gene="gltA"
                     /locus_tag="ACHU0J_002317"
     CDS             complement(88..1371)
                     /gene="gltA"
                     /locus_tag="ACHU0J_002317"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1216992.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1577..1684)
                     /locus_tag="ACHU0J_002318"
     CDS             complement(1577..1684)
                     /locus_tag="ACHU0J_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1216993.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2065..2469
                     /gene="sdhC"
                     /locus_tag="ACHU0J_002319"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2065..2469
                     /gene="sdhC"
                     /locus_tag="ACHU0J_002319"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1216994.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2463..2810
                     /gene="sdhD"
                     /locus_tag="ACHU0J_002320"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2463..2810
                     /gene="sdhD"
                     /locus_tag="ACHU0J_002320"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1216995.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2810..4576
                     /gene="sdhA"
                     /locus_tag="ACHU0J_002321"
     CDS             2810..4576
                     /gene="sdhA"
                     /locus_tag="ACHU0J_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1216996.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4592..5308
                     /gene="sdhB"
                     /locus_tag="ACHU0J_002322"
     CDS             4592..5308
                     /gene="sdhB"
                     /locus_tag="ACHU0J_002322"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1216997.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5609..8410
                     /gene="sucA"
                     /locus_tag="ACHU0J_002323"
     CDS             5609..8410
                     /gene="sucA"
                     /locus_tag="ACHU0J_002323"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1216998.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8425..9642
                     /gene="odhB"
                     /locus_tag="ACHU0J_002324"
     CDS             8425..9642
                     /gene="odhB"
                     /locus_tag="ACHU0J_002324"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1216999.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            9736..10902
                     /gene="sucC"
                     /locus_tag="ACHU0J_002325"
     CDS             9736..10902
                     /gene="sucC"
                     /locus_tag="ACHU0J_002325"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1217000.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10902..11771
                     /gene="sucD"
                     /locus_tag="ACHU0J_002326"
     CDS             10902..11771
                     /gene="sucD"
                     /locus_tag="ACHU0J_002326"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1217001.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11874..12596)
                     /gene="mngR"
                     /locus_tag="ACHU0J_002327"
     CDS             complement(11874..12596)
                     /gene="mngR"
                     /locus_tag="ACHU0J_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1217002.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12766..14682
                     /gene="mngA"
                     /locus_tag="ACHU0J_002328"
     CDS             12766..14682
                     /gene="mngA"
                     /locus_tag="ACHU0J_002328"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1217003.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14700..17335
                     /gene="mngB"
                     /locus_tag="ACHU0J_002329"
                     /pseudo
     CDS             14700..17335
                     /gene="mngB"
                     /locus_tag="ACHU0J_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
BASE COUNT         4324 a         4417 c         4802 g         4215 t
ORIGIN      
        1 ttttacaatt ttttgtgttt aggcatataa aaattaaccc gccatttgaa cggcgggtta
       61 aaatatttac aacttagcaa tcaaccatta acgcttgata tcgcttttaa agtcgcgttt
      121 ttcatatcct gtatacagct gacgcggacg ggcaatcttc ataccgtaac tgtgcatttc
      181 gctccagtgg gcgatccagc caacggtacg tgccattgcg aaaatgacgg tgaacatgga
      241 agacggaata cccatcgctt tcaggatgat accagagtag aaatcgacgt tcgggtacag
      301 tttcttctcg ataaagtacg ggtcgttcag cgcgatgttt tccagctcca tagccacttc
      361 cagcaggtca tctttggtgc ccagctcttt cagaacttca tggcaggttt cacgcattac
      421 ggtggcgcgc gggtcgtaat ttttgtacac gcggtgaccg aagcccatca ggcggaaaga
      481 atcattttta tctttcgcac gacgaacaaa ttccggaatg tgtttaacgg agctaatttc
      541 ttccagcatt ttcagcgccg cttcgttagc accaccgtgc gcaggtcccc acagtgaagc
      601 aatacctgct gcgatacagg caaacgggtt cgcccccgaa gagccagcgg tacgcacggt
      661 ggaggtagag gcgttctgtt catggtcagc gtgcaggatc agaatacggt ccatagcacg
      721 ttccagaatc ggattaactt catacggttc gcacggcgtg gagaacatca tattcaggaa
      781 gttaccggca taggagagat cgttgcgcgg ataaacaaat ggctgaccaa tggaatactt
      841 gtaacacatc gcggccatgg tcggcatttt cgacagcagg cggaacgcgg caatttcacg
      901 atgacgagga ttgttaacat ccagcgagtc gtgatagaac gccgccagcg cgccggtaat
      961 accacacatg actgccattg gatgtgagtc gcgacggaaa gcgtggaaca gacgggtaat
     1021 ctgctcgtgg atcatggtat gacgggtcac cgtagtttta aattcgtcat actgttcctg
     1081 agtcggtttt tcaccattca gcaggatgta acaaacttct aggtagttag aatcggtcgc
     1141 cagctgatcg atcgggaaac cgcggtgcag caaaatacct tcatcaccat caataaaagt
     1201 aattttagat tcgcaggatg cggttgaagt gaagcctggg tcaaaggtga acacaccttt
     1261 tgaaccgaga gtacggatat caataacatc ttgacccagc gtgcctttca gcacatccag
     1321 ttcaacagct gtgtccccgt tgaggttgag ttttgctttt gtatcagcca tttaaggtct
     1381 ccttagcgcc ttattgcgta agactgccgg aacttaaatt tgccttcgca catcaacctg
     1441 gctttacccg tttgttattt ggctcgccgc tctgtgaaag aggggaaaac ctgggtacag
     1501 agctctgggc gcttgcaggt aaagaatcca ttgatgacga ataaatggcg aatcaagtac
     1561 ttagcaatcc gaattattaa acttgtctac cactaataac tgtcccgaat gaattggtca
     1621 atacttccac actgttacat aagttaatct taggtgaaat accgacttca taacttttac
     1681 gcattatatg cttttcctgg taatgtttgt aacaactttg ttgaatgatt gtcaaattag
     1741 atgattaaaa attaaataaa tgttgttatc gtgacctgga tcactgttca ggataaaacc
     1801 cgacaaacta tatgtaggtt aattgtaatg attttgtgaa cagcctatac tgccgccagg
     1861 tctccggaac accctgcaat cccgagccac ccagcgttgt aacgtgtcgt tttcgcatct
     1921 ggaagcagtg ttttgcatga cgcgcagtta tggaaaggac gctgtctgac ccgcaagcag
     1981 accggaggaa ggaaatcccg acgtctccag gtaacagaaa gttaacctct gtgcccgtag
     2041 tccccaggga ataataagaa cagcatgtgg gcgttattca tgataagaaa tgtgaaaaaa
     2101 caaagacctg ttaatctgga cctacagacc atccggttcc ccatcacggc gatagcgtcc
     2161 attctccatc gcgtttccgg tgtgatcacc tttgttgcag tgggcatcct gcagtggctt
     2221 ctgggtacca gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat tatgggcagc
     2281 ttcttcgtca aatttatcat gtggggcatc cttaccgctc tggcgtatca cgtcgtcgta
     2341 ggtattcgcc acatgatgat ggattttggc tatctggaag aaacattcga agcgggtaaa
     2401 cgctccgcca aaatctcctt tgttattact gtcgtgcttt cacttctcgc aggagtcctc
     2461 gtatggtaag caacgcctcc gcattaggac gcaatggcgt acatgatttc atcctcgttc
     2521 gcgctaccgc tatcgtcctg acgctctaca tcatttatat ggtcggtttt ttcgccacca
     2581 gtggcgaact gacatatgaa gtctggatcg gtttcttcgc ctctgcgttc accaaagtgt
     2641 tcaccctgct ggcgctgttt tctatcttga ttcatgcctg gatcggcatg tggcaggtgt
     2701 tgaccgacta cgttaaaccg ctggccttgc gcctgatgct gcaactggtg attgtcgttg
     2761 cactggtggt ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga tgaaattgcc
     2821 agtcagagaa tttgatgcag ttgtgattgg tgccggtggc gcaggtatgc gcgcggcgct
     2881 gcaaatttcc cagagcggcc agacctgtgc cctgctctct aaagtcttcc cgacccgttc
     2941 ccataccgtt tctgcgcaag gcggcattac cgttgcgctg ggtaataccc atgaagataa
     3001 ctgggaatgg catatgtacg acaccgtaaa agggtcggac tatatcggtg accaggacgc
     3061 gattgaatat atgtgtaaaa ccgggccgga agcgattctg gaactcgaac acatgggcct
     3121 gccgttctcg cgtctcgatg atggtcgtat ctatcaacgt ccgtttggcg gtcagtcgaa
     3181 aaacttcggc ggcgagcagg cggcacgcac tgcggcggct gctgaccgta ccggtcacgc
     3241 actgttgcac acgctttatc agcagaacct gaaaaaccac accaccattt tctccgagtg
     3301 gtatgcgctg gatctggtga aaaaccagga tggcgcggtg gtgggttgta ccgcactgtg
     3361 catcgaaacc ggtgaagtgg tttatttcaa agcccgcgct accgtgctgg cgactggcgg
     3421 agcagggcgt atttatcagt ccaccactaa cgcccacatt aacactggcg acggtgtcgg
     3481 catggctatc cgcgcaggcg taccggtgca ggatatggaa atgtggcaat tccacccgac
     3541 cggcattgcc ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag gcggttatct
     3601 gctgaacaaa catggcgaac gctttatgga gcgttatgcg ccgaacgcca aagacctggc
     3661 gggccgtgat gtggtggcgc gttccatcat gattgaaatc cgtgaaggtc gcggctgtga
     3721 tggtccgtgg gggccacacg cgaaactgaa actcgatcac ctgggtaaag aagttctcga
     3781 atcccgtctg ccgggtatcc tggagctttc ccgtaccttc gctcacgtcg atccggtgaa
     3841 agagccgatt ccggttatcc caacctgtca ctacatgatg ggcggtattc cgaccaaagt
     3901 taccggtcag gcgctgactg tgaatgagaa aggcgaagat gtggttgttc cgggactgtt
     3961 tgccgttggt gaaatcgctt gtgtatcggt acacggcgct aaccgtctgg gcggcaactc
     4021 gctgctggac ctggtggtct ttggtcgcgc ggcaggtctg catctgcaag agtctatcgc
     4081 cgagcagggc gcactgcacg atgccagcga gtctgatgtt gaagcgtctc tggatcgcct
     4141 gaaccggtgg aacaataatc gtaacggtga agatccggtg gcgatccgta aagcgctgca
     4201 agaatgtatg cagcataact tctcggtctt ccgtgaaggt gatgcgatgg cgaaagggct
     4261 tgagcagttg aaagtgatcc gcgagcgtct gaaaaatgcc cgtctggatg acacttccag
     4321 cgagttcaac acccagcgcg ttgagtgcct ggaactggat aacctgatgg aaacggcgta
     4381 tgcaacggct gtttctgcca acttccgtac cgaaagccgt ggcgcacata gccgcttcga
     4441 cttcccggat cgtgatgatg aaaactggct gtgccactcc ctgtatctgc ctgagtcgga
     4501 atccatgacg cgccgaagcg tcaacatgga accgaaactg cgcccggcat tcccgccgaa
     4561 gattcgtact tactaatgcg gagacaggaa aatgagactc gagttttcaa tttatcgcta
     4621 taacccggat gttgatgatg ctccgcgtat gcaggattac accctggaag cggaagaagg
     4681 tcgcgacatg atgctgctgg atgcgcttat ccagctgaaa gagaaagatc ccagcctgtc
     4741 gttccgccgc tcctgccgtg aaggtgtgtg cggttccgac ggtctgaaca tgaacggcaa
     4801 gaatggtctg gcctgtatta ccccgatttc ggcactcaac cagccgggca agaagattgt
     4861 gattcgcccg ctgccaggtt taccggtgat ccgcgatttg gtggtagaca tgggacaatt
     4921 ctatgcgcaa tatgagaaaa ttaagcctta cctgttgaat aatggacaaa atccgccagc
     4981 tcgcgagcat ttacagatgc cagagcagcg cgaaaaactc gacgggctgt atgaatgtat
     5041 tctctgcgca tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg ataagtttat
     5101 cggcccggca ggcttgttag cggcatatcg tttcctgatc gatagccgtg ataccgagac
     5161 tgacagccgc ctcgacggtt tgagcgatgc attcagcgta ttccgctgtc acagcatcat
     5221 gaactgcgtc agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg gccatatcaa
     5281 gtcgatgttg ttgcaacgta atgcgtaaac tgtaggcctg ataagacgcg caagcgttgc
     5341 atcaggcaac cagtgccgga tgcggcgtga acgccttatc cggcctacaa gtcattaccc
     5401 gcaggcctga taagcgcagc gcatcaggcg taacaaagaa atgcaggaaa tctttaaaaa
     5461 ctgcccctga cactaagaca gtttttaaag gttccttcgc gagccactac gtagacaaga
     5521 gctcgcaagt gaaccccggc acgcacatca ctgtgcgtgg tagtatccac ggcgaagtaa
     5581 gcataaaaaa gatgcttaag ggatcacgat gcagaacagc gctttgaaag cctggttgga
     5641 ctcttcttac ctctctggcg caaaccagag ctggatagaa cagctctatg aagacttctt
     5701 aaccgatcct gactcggttg acgctaactg gcgttcgacg ttccagcagt tacctggtac
     5761 gggagtcaaa ccggatcaat tccactctca aacgcgtgaa tatttccgcc gcctggcgaa
     5821 agacgcttca cgttactctt caacgatctc cgaccctgac accaatgtga agcaggttaa
     5881 agtcctgcag ctcattaacg cataccgctt ccgtggtcac cagcatgcga atctagatcc
     5941 gctgggactg tggcagcaag ataaagtggc cgatctggat ccgtctttcc acgatctgac
     6001 cgaagcagac ttccaggaaa ccttcaacgt cggttcattt gccagcggca aagaaaccat
     6061 gaaactcggc gagcttattt ccgccctcaa gcaaacctac tgcggcccga ttggtgccga
     6121 gtatatgcac atcaccagca ccgaagaaaa acgctggatc caacagcgta tcgagtctgg
     6181 tcgcgcgact ttcaatagcg aagagaaaaa acgcttctta agcgaactga ccgccgctga
     6241 aggccttgag cgttacctcg gcgcaaaatt ccctggcgca aaacgcttct cgctggaagg
     6301 cggtgacgcg ttaatcccga tgcttaaaga gatgatccgc cacgctggca acagcggcac
     6361 ccgcgaagtg gttctcggga tggcgcaccg tggtcgtctg aacgtgctgg tgaacgtgct
     6421 gggtaaaaaa ccgcaagact tgttcgacga gttcgccggt aaacataaag aacacctcgg
     6481 cacaggcgac gtgaaatacc acatgggctt ctcgtctgac ttccagaccg atggcggcct
     6541 ggtgcacctg gcgctggcgt ttaacccgtc tcaccttgag attgtaagcc cggtcgttat
     6601 cggctctgtt cgtgcccgtc tggacagact tgatgagccg aacagcaaca aagtgctgcc
     6661 aatcaccatc cacggtgacg ccgcagtgac cgggcagggc gtggttcagg aaactctgaa
     6721 catgtcgaaa gcgcgtggtt atgaagttgg cggtacggta cgtatcgtta tcaacaacca
     6781 ggttggtttc accacctcta acccgctgga tgcccgttct acaccgtact gtactgatat
     6841 cggtaagatg gttcaggcac cgattttcca cgttaacgcg gatgatccgg aagccgttgc
     6901 ctttgtgacc cgtctggcgc tcgatttccg taacaccttt aaacgtgatg tcttcatcga
     6961 cctggtatgc taccgccgtc acggccacaa cgaagccgac gagccgagcg caacccagcc
     7021 actgatgtat cagaaaatca aaaaacatcc gacaccgcgc aaaatctacg ctgacaagct
     7081 ggagcaggaa aaagtggcga cgctggaaga tgccaccgag atggttaacc tgtaccgcga
     7141 tgcgctggat gctggcgatt gcgtagtggc agagtggcgt ccgatgaaca tgcactcttt
     7201 cacctggtcg ccgtacctca accacgaatg ggacgaagag tacccgaaca aagttgagat
     7261 gaagcgcctg caggaactgg ctaaacgtat cagtacggta ccagaagcag ttgaaatgca
     7321 gtctcgcgtt gccaagattt atggcgatcg ccaggcgatg gcagccggtg agaaactgtt
     7381 cgactggggc ggtgcggaaa acctcgctta cgccacgctg gttgatgaag gcattccggt
     7441 tcgcctgtcg ggtgaagact ccggtcgcgg taccttcttc caccgccacg cggtgatcca
     7501 caaccagtct aacggttcca cttacacgcc gctgcaacac atccataacg gtcagggcgc
     7561 gttccgtgtc tgggactccg tactgtcaga agaagccgtg ctggcgtttg aatatggtta
     7621 tgccaccgca gaaccacgca ccctgaccat ctgggaagcg cagttcggtg acttcgccaa
     7681 cggtgcgcag gtggttatcg accagttcat ctcctctggc gagcagaaat ggggccggat
     7741 gtgtggtctg gtgatgttgc tgccgcacgg ttacgaaggg caggggccgg agcactcctc
     7801 cgcgcgtctg gaacgttatc tgcaactttg tgctgagcaa aacatgcagg tttgcgtacc
     7861 gtctaccccg gcacaggttt accacatgct gcgtcgtcag gcgctgcgcg ggatgcgtcg
     7921 tccgctggtc gtgatgtcgc cgaaatccct gctgcgtcat ccgctggcgg tttccagcct
     7981 cgaagaactg gcgaacggca ccttcctgcc agccatcggt gaaatcgacg agcttgatcc
     8041 gaagggcgtg aagcgcgtag tgatgtgttc tggtaaggtt tattacgacc tgctggaaca
     8101 gcgtcgtaag aacaatcaac acgatgtcgc cattgtgcgt atcgagcaac tctacccgtt
     8161 cccgcataaa gcgatgcagg aagtgttgca gcagtttgct cacgtcaagg attttgtctg
     8221 gtgccaggaa gagccgctca accagggcgc atggtactgc agccagcatc atttccgtga
     8281 agtgattccg tttggggctt ctctgcgtta tgcaggccgc ccggcctccg cctctccggc
     8341 ggtagggtat atgtccgttc accagaaaca gcaacaagat ctggttaatg acgcgctgaa
     8401 cgtcgaataa ataaaggata cacaatgagt agcgtagata ttctggtccc tgacctgcct
     8461 gaatccgtag ccgatgccac cgtcgcaacc tggcataaaa aacccggcga cgcagtcgta
     8521 cgtgatgaag tgctggtaga aatcgaaact gacaaagtgg tactggaagt accggcatca
     8581 gcagacggca ttctggatgc ggttctggaa gatgaaggta caacggtaac gtctcgtcag
     8641 atccttggtc gcctgcgtga aggcaacagc gccggtaaag aaaccagcgc caaatctgaa
     8701 gagaaagcgt ccactccggc gcaacgccag caggcgtctc tggaagagca aaacaacgat
     8761 gcgttaagcc cggcgatccg tcgcctgctg gctgaacaca atctcgacgc cagcgccatt
     8821 aaaggcaccg gtgtgggtgg tcgtctgact cgtgaagatg tggaaaaaca tctggcgaaa
     8881 gccccggcga aagagtctgc tccggcagcg gctgctccgg cggcgcaacc ggctctggct
     8941 gcacgtagtg aaaaacgtgt cccgatgact cgcctgcgta agcgtgtggc agagcgtctg
     9001 ctggaagcga aaaactccac cgccatgctg accacgttca acgaagtcaa catgaagccg
     9061 attatggatc tgcgtaagca gtacggtgaa gcgtttgaaa aacgccacgg catccgtctg
     9121 ggctttatgt ccttctacgt gaaagcggtg gttgaagccc tgaaacgtta cccggaagtg
     9181 aacgcgtcta tcgacggcga tgacgtggtt taccacaact atttcgacgt cagcatggcg
     9241 gtttctacgc cgcgcggcct ggtgacgccg gttctgcgtg atgtcgatac cctcggcatg
     9301 gcagacatcg agaagaaaat caaagagctg gcagtcaaag gccgtgatgg caagctgacg
     9361 gttgaagatc tgaccggtgg taacttcacc atcaccaacg gtggtgtgtt cggttccctg
     9421 atgtctacgc cgatcatcaa cccgccgcag agcgcaattc tgggtatgca cgctatcaaa
     9481 gatcgtccga tggcggtgaa tggtcaggtt gagatcctgc cgatgatgta cctggcgctg
     9541 tcctacgatc accgtctgat cgatggtcgc gaatccgtgg gcttcctggt aacgatcaaa
     9601 gagttactgg aagagccgac gcgtctgctg ctggacgtgt agtagtttaa gcttcacctg
     9661 cactgtagac cgacgtggat agggtgtcgg tctacggttt aaaagataac gattactgaa
     9721 ggatggacag aacacatgaa cttacatgaa tatcaggcaa aacaactgtt tgcccgctat
     9781 ggcttaccag caccggtggg ttatgcctgt actactccgc gcgaagcaga agaagccgct
     9841 tcaaaaatcg gtgccggtcc gtgggtagtg aaatgtcagg ttcacgctgg tggccgcggt
     9901 aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt tgcagaaaac
     9961 tggctgggca agcgtctggt aacgtatcaa acagatgcca atggccaacc ggttaaccag
    10021 attctggttg aagcggcgac cgatatcgct aaagagctgt atctcggtgc cgttgtagac
    10081 cgtagttccc gtcgcgtggt ctttatggcc tccaccgaag gcggcgtgga aatcgaaaaa
    10141 gtggcggaag aaactccgca cctgatccat aaagttgcgc ttgatccgct gactggcccg
    10201 atgccgtatc agggacgcga gctggcgttc aaactgggtc tggaaggtaa actggttcag
    10261 cagttcacca aaatcttcat gggcctggcg accattttcc tggagcgcga cctggcgctg
    10321 atcgaaatca acccgctggt catcaccaaa cagggcgatc tgatttgcct cgacggcaaa
    10381 ctgggcgctg atggcaacgc cctgttccgc cagcctgatc tgcgcgaaat gcgtgaccag
    10441 tcgcaggaag atccgcgtga agcacaggct gcacagtggg aactgaacta cgttgcgctg
    10501 gacggtaaca tcggttgtat ggttaacggc gcaggtctgg cgatgggtac gatggacata
    10561 gttaaactgc acggcggcga accggctaac ttcctggacg ttggcggcgg cgcaaccaaa
    10621 gaacgtgtaa ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa agccgttctg
    10681 gttaacatct tcggcggtat cgttcgttgc gacctgatcg ctgacggtat catcggcgcg
    10741 gtagcggaag tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa caacgccgaa
    10801 ctcggcgcga agaaactggc tgacagcggc ctgaatatta ttgcagcaaa aggtctgacg
    10861 gatgcagctc agcaggttgt tgccgcagtg gaggggaaat aatgtccatt ttaattgata
    10921 aaaacaccaa ggttatctgc cagggcttta ccggtagcca ggggactttc cactcagaac
    10981 aggccattgc atacggcact aaaatggttg gtggcgtaac tccaggtaaa ggcggcacta
    11041 cccacctcgg cctgccggtg ttcaacaccg tgcgtgaagc tgttgctgct actggcgctt
    11101 ccgcgtctgt tatttacgta ccagcaccgt tctgcaaaga ctccattctg gaagctatcg
    11161 atgcaggcat caaactgatt atcaccatca ctgaaggcat cccgacgctg gatatgctga
    11221 ccgtgaaagt gaaactggac gaagcaggcg ttcgtatgat cggcccgaac tgcccaggcg
    11281 ttatcacccc gggtgaatgc aaaattggta tccagccggg tcacattcac aaaccgggta
    11341 aagtgggtat cgtttcccgt tccggtacac tgacctatga agcggttaaa cagaccacgg
    11401 attacggttt cggtcagtcg acctgtgtcg gtatcggcgg tgacccgatc cctggctcta
    11461 actttatcga cattctcgaa atgttcgaaa aagatccgca gaccgaagcg atcgtgatga
    11521 tcggtgagat cggcggtagc gctgaagaag aagcggcagc gtacatcaaa gagcatgtta
    11581 ccaagccagt tgtgggttac atcgctggtg tgactgcgcc gaaaggcaaa cgtatgggcc
    11641 acgcgggtgc gatcattgcc ggtgggaaag ggactgcgga tgagaaattc gctgctctgg
    11701 aagccgcagg cgtgaaaacc gttcgcagcc tggcggatat cggtgaagca ctgaaaactg
    11761 ttctgaaata aatatctgta ataagaaata gccctcgccg cttccctcta caggaatggc
    11821 gaagggctgt cggtttcgac atggttggcc atcgtatgat ggcctttttg tgcttatcgc
    11881 gatgattttc gctgcgctat cagggtaaat ttatagtcat cggtattaaa agcgttgcgg
    11941 ctatattcaa acacccgacc atcaactaaa tatccacgcg atactttttc aagaatcggc
    12001 tttgtctggc tgatattaag cagacggctc atctcttcgg ttggcatcag aggaatgatt
    12061 tcctgttcgc tacgatcgat aaccatcttc ttcacttctt caataaagtg atatttcgaa
    12121 ttttccatga cctgccaggt gagatccggg aacaacgcaa gcggcatcca ggtttcttcc
    12181 agcgccattg gcttttgctt gcgatagcgc acgcgcttca catgccaaac acgatcctgc
    12241 acggtgattt gtagctgttg ctgaagaaaa tcgtcagccg gaatcacttc aaatatcaga
    12301 acttcactgt gtgtatcgac gtgacggtcc gacagttttt catcaaaact ggttaactga
    12361 aaaatatcgt aattgacccg ctcttctttg acgtaagtcc cgctgccctg aatgctttcg
    12421 aggatctgtt gctcgactaa ctggcgcaaa gcctgacgca ccgtaacccg gctgacgcca
    12481 aactctgttt gtaatgccga ttcggtaggt aacgcatcgc caggtttaag ctcgccacgc
    12541 gcaatttgct cacgaatgcg atcagcaatc tgtcggtata agggtttgtg tcccattatt
    12601 tagcatctca ttaatacgaa tttagccatt atgcccgata aactcatcct gtaaacaata
    12661 caaataaaat gcaaataatt tcaatcaagt gaaattgatt acgcaatggt attgttttgt
    12721 cgggcacact ggcgcgacta taaaaacgat caagtgagga tcatgatgaa cctgacgact
    12781 ctgacccacc gcgatgcgtt gtgtctgaat gcgcgcttta ccagccgtga agaggccatt
    12841 cacgcgttga ctcaacgtct tgctgctctg gggaaaattt ccagtactga gcaatttctg
    12901 aaagaagtgt atcgccgtga aagccttggc ccgaccgcct taagtgaagg gttggctgtg
    12961 ccgcatggca aaactgctgc ggtaaaagaa gcggcgtttg cagtcgccac actcagcgag
    13021 ccgcttcagt gggaaggcgt ggatggcccg gaagcggttg atttagtggt gttgctggcg
    13081 attcccctca atgaagcggg tactacgcac atgcaactgc tgacagcgct gaccacgcgc
    13141 ctggcggatg atgagattcg ggcgcgtata cagtcggcga cgacgcctga tgaattgctc
    13201 tcggcgctgg atgacaaggg aggcacgcaa ccttctgcct ctttttccaa cgcgccaact
    13261 atcgtttgcg taacggcctg cccggcgggt attgctcaca cctacatggc tgcggagtat
    13321 ctggaaaaag ccggacgcaa actcggcgtg aatgtctacg ttgaaaagca aggtgctaac
    13381 ggtattgaag ggcgtttaac ggcggatcaa ctcaatagtg caactgcctg tatttttgcg
    13441 gctgaagtcg ccatcaagga gagtgagcgt tttaacggca ttcctgcgct ttctgtgcct
    13501 gttgccgagc cgattcgcca tgcagaagcg ttgatccaac aatcgcttac cctcgagcgt
    13561 ggcgatgaga cgcgtacctt acagcaagat acgcaaccgg tgaaaagcgt caaaacggag
    13621 ctgaaacagg cactgttgag cgggatctct tttgccgtac cgttgattgt cgcggggggg
    13681 acggtgctgg cggtcgcggt attactgtcg caaatcttcg ggctacaaga tctgtttaat
    13741 gaagaaaact cctggctgtg gatgtaccgc aaactgggcg gcgggctgct cggaattttg
    13801 atggtaccgg tgctggcagc ctataccgcc tattcgctgg cagataaacc ggcgttagcg
    13861 ccaggctttg cggctggact tgccgccaac atgatcggct ccgggtttct cggcgcggtc
    13921 gttggcggat tgatagccgg ttacttgata cgctggatga aaaatcactt gcgtcttagc
    13981 agtaaattca atggcttcct gactttttat ctctacccgg tgctcggtac attgggagcg
    14041 ggcagtctga tgctgtttgt ggtgggggaa cctgtcgcct ggatcaataa ctcgcttacc
    14101 gcctggctga acggtctgtc aggaagtaac gcgctgttgc tgggtgccat tctcggtttt
    14161 atgtgttcct ttgaccttgg agggccagtg aataaagccg cttatgcatt ctgcctgggc
    14221 gcaatggcga acggcgttta cggcccgtat gccattttcg cctccgtcaa aatggtttcg
    14281 gcatttaccg taaccgcttc cacgatgctc gcaccgcgcc tgtttaaaga gtttgaaatt
    14341 gagaccggga aatccacctg gctgttaggg ctggcaggta ttaccgaagg ggcgatcccg
    14401 atggcgattg aagatccgct gcgggttatt ggttcgtttg tgctgggctc tatggtaacg
    14461 ggcgctattg tcggtgcgat gaatatcggc ctttcgacac ccggtgctgg cattttctcg
    14521 ctctttttac ttcatgataa tggcgcgggc ggtgttatgg cggcaattgg ctggtttggt
    14581 gcggcattgg tgggggctgc aatctcgact gcaattctcc tgatctggcg gcgtcacgcg
    14641 gttaagcatg gcaactatct gactgatggc gtaatgccat aaacaaaaag gaaacgacga
    14701 tgaaagcggt atctcgcgtt cacatcaccc cgcatatgca ctgggatcgg gagtggtatt
    14761 tcaccaccga agagtcacgc attctgctgg tcaataatat ggaagagatc ctgtgccgac
    14821 tggaacagga caacgaatac aaatattacg tactcgacgg gcaaacggcg atcctcgaag
    14881 attatttcgc ggtgaaaccg gaaaacaaag accgcttgaa gaaactggta gaagccggca
    14941 agttgattat cggcccctgg tatacccaga ccgataccac gattgtttct gcggaatcca
    15001 tcgtccgtaa tctgatgtac ggaatgcgtg actgcctcgc gtttggcgag ccgatgaaaa
    15061 taggttattt acccgattcc tttggcatgt ccgggcaact gccgcatatc tacaatggat
    15121 ttggcattac ctgcaccatg ttctggcgtg gatgttctga gcgccacggt acggataaaa
    15181 ccgagttttt gtggcaaagc agtgacggta gcgaagtgac ggcgcaggtg atgccgttgg
    15241 gttatgccat cggtaagtac ttacctgcgg acgaaaacgg attacgcaaa cgcctcgaca
    15301 gttattttga cgtgctggaa aaagcgtctg taaccaaaga gattttgctg ccgaatgggc
    15361 atgatcagat gccattgcag caaaatatct tcgaagtgat ggagaaactg cgcgaaattt
    15421 atcctcaacg aaagtttgtg atgagccgct ttgaagaggt atttgagaag atcgaagcgc
    15481 agcgagataa tctggcaacc ctgaaagggg aatttattga tggcaaatat atgcgcgtgc
    15541 atcgcaccat cggttccacg cgcatggata tcaaagttgc ccacgcacgt attgaaaata
    15601 agattgttaa tctgctggaa ccgctggcaa cgctggccag gacgttgggt tttggatacc
    15661 accacggctt gctggagaaa atgtggaaag agatcttaaa aaatcatgcc cacgacagca
    15721 tcggctgctg ctgtcgtgac aaagttcatc gcgaaatcgt cgcccgcttc gaactggctg
    15781 aagacatggc ggataatctg ctgcgtttct atatgcgtaa aatcgccgac aacatgccgc
    15841 agagcgacgc cgacaaactc gtcctgttta acctgatgcc ctggccgcgg gaagaagtga
    15901 tcaacaccac tgtgcggctg cgcgccagcc agtttaatct acgagacgat cgcggtcagc
    15961 ctgtaccgta ttttattcgc catgcccgtg agatcgatcc aggcctaatc gatcggcaaa
    16021 tagttcatta cggtaattac gatcccttta tggagtttga tatacagatc aaccagattg
    16081 tcccttctat gggctatcgc acgctttata tcgaagcgaa tcagcctggc aacgtgattg
    16141 cggcaaaaag tgacgctgaa gggatactgg aaaatgcttt ctggcaaatt gcgctcaatg
    16201 aggatggaac gctacaactg gtagataaag atagcggtgt gcgctatgac cgggtgttgc
    16261 aaattgaaga aagctctgat gatggtgatg aatatgacta ttcacccgca aaagaagagt
    16321 gggtaattac ttcggcgaat gcgaaaccgc aatgcgagat tattcatgag gcctggcaga
    16381 gcagggctgt tatccgctat gaaatggcgg tgccgcgtaa tctgcaacaa cgcagcgcaa
    16441 gacaatccac tggcagagta gatgtcgagt tggttgtcac tcttagtcat aacaacaggc
    16501 gcattgatgt ggatatcaac cttgataacc aggctgacga tcatcgcctt cgcgtcctga
    16561 tccctacatc ttttaacacc gatagtgttc tggcagatac ccagtttggt tcgctaacgc
    16621 gccccgtgaa cgacagtgca atgaacaact ggcagcaaga aagctggaaa gaagcgccgg
    16681 ttccggtatg gaatatgctc aactatgcag ccttacagga agggcgtaac ggcatggctg
    16741 tctttagcga agggctacgt gaatttgaag tcatcggtga agagaagaaa acctttgcca
    16801 ttacgttgct gcgcggcgtg ggcttactgg ggaaagaaga tctgctttta aggcctaggc
    16861 ggccttcggg aattaaaatg ccagtcccgg actcacaact acgtggtctg ctttcttgtc
    16921 gcctaagttt attgagttat accggtacgc caaccgccgc tggtgtagct cagcaggcgc
    16981 gagcatggct gactccagta cagtgttaca acaaaatccc atgggatgcg atgaagctca
    17041 acaaagccgg attcaacgtg ccggaaagtt atagtttgtt gaaaatgccc ccagtgggat
    17101 gcccctgata agcgcactta agaaagctga agaccgacaa gaagtgattt tacggctgtt
    17161 taatccggct gaatcagcaa cctgtgatgc gactgttgct ttcagtcgcg aggtgatttc
    17221 ttgctcagaa acgatgatgg atgaacacat taccaccgag gaaaatcaag gttcaaatct
    17281 atcggggcct tttttacccg gccagtcacg gacgttcagt taccggcttg cctgaatagc
    17341 aatcaaaccg aagccacata tgcgcggcca gattgttgac aaagggcgct ttgttcatgc
    17401 cggatacggc atgaacgctt tattcggtct acaaaagcag gcaaattcaa tatattgcag
    17461 agatgatgta gacactggca agcgtagcgc atcaggtaat tttgtgttta tcttcactct
    17521 caagctacgt atatgtggct ttatttttaa caaaataata accacgggtg agttaattat
    17581 aatataatta taagttaatt aaatgttaat atcggcggga gggagattta tgcctttatt
    17641 aataatcctg aaactctgcg tcgtattagc cagtgaccaa aaaaagaatt aaggtcaacc
    17701 gtgctgtttt tgcttcgtct ctttttatct ttaattgcca accgaaacta atttcagt
//
LOCUS       DAZPDE010000079        17677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPDE010000079 DAZPDE010000000
VERSION     DAZPDE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.079"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            73..756
                     /locus_tag="ACHU0J_002330"
     CDS             73..756
                     /locus_tag="ACHU0J_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1217004.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            800..5462
                     /locus_tag="ACHU0J_002331"
                     /pseudo
     CDS             800..5462
                     /locus_tag="ACHU0J_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5830..7485
                     /gene="lldP"
                     /locus_tag="ACHU0J_002332"
     CDS             5830..7485
                     /gene="lldP"
                     /locus_tag="ACHU0J_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1217005.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7485..8261
                     /gene="lldR"
                     /locus_tag="ACHU0J_002333"
     CDS             7485..8261
                     /gene="lldR"
                     /locus_tag="ACHU0J_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1217006.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8258..9448
                     /gene="lldD"
                     /locus_tag="ACHU0J_002334"
     CDS             8258..9448
                     /gene="lldD"
                     /locus_tag="ACHU0J_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1217007.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9644..10117
                     /gene="trmL"
                     /locus_tag="ACHU0J_002335"
     CDS             9644..10117
                     /gene="trmL"
                     /locus_tag="ACHU0J_002335"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1217008.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10170..10991)
                     /gene="cysE"
                     /locus_tag="ACHU0J_002336"
     CDS             complement(10170..10991)
                     /gene="cysE"
                     /locus_tag="ACHU0J_002336"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1217009.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11071..12090)
                     /gene="gpsA"
                     /locus_tag="ACHU0J_002337"
     CDS             complement(11071..12090)
                     /gene="gpsA"
                     /locus_tag="ACHU0J_002337"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1217010.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12090..12557)
                     /gene="secB"
                     /locus_tag="ACHU0J_002338"
     CDS             complement(12090..12557)
                     /gene="secB"
                     /locus_tag="ACHU0J_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1217011.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12620..12871)
                     /gene="grxC"
                     /locus_tag="ACHU0J_002339"
     CDS             complement(12620..12871)
                     /gene="grxC"
                     /locus_tag="ACHU0J_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1217012.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13013..13444)
                     /locus_tag="ACHU0J_002340"
     CDS             complement(13013..13444)
                     /locus_tag="ACHU0J_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1217013.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13689..15233
                     /gene="gpmM"
                     /locus_tag="ACHU0J_002341"
                     /gene_synonym="pgmI"
     CDS             13689..15233
                     /gene="gpmM"
                     /locus_tag="ACHU0J_002341"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1217014.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15267..16526
                     /gene="envC"
                     /locus_tag="ACHU0J_002342"
     CDS             15267..16526
                     /gene="envC"
                     /locus_tag="ACHU0J_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1217015.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16530..17489
                     /locus_tag="ACHU0J_002343"
     CDS             16530..17489
                     /locus_tag="ACHU0J_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1217016.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17476..17677)
                     /locus_tag="ACHU0J_002344"
                     /pseudo
     CDS             complement(17476..17677)
                     /locus_tag="ACHU0J_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4410 a         4601 c         4636 g         4030 t
ORIGIN      
        1 atagctcacc aataatacaa tggacaactc taccggcatt gagcgctggt cggaggaaat
       61 aaggattttt acatgaactt gaagaagatt tttttcagtg ctgtcactgt ttcagttctc
      121 tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc cagaaagtag tttactgaaa
      181 caacaagaag aacataataa taaaattgtg ctacttgaaa aacaacaagc acagctaaag
      241 agtcaactcg aaactattca aaaacaacaa actggcataa taaacagtac aaagacttta
      301 actcacgtaa ttaaatccgt taaagaccaa caaaatactt ttatttttac cgagtttaac
      361 ccagcaaaaa cgaagtattt catcctgaac aacggttcgg tggctttagc gggtcgagtg
      421 ttatccattg acgccacaga gaatggtagt gttattcata tctcactggt caacttatta
      481 agtacaccta tctcaaacat tggttttaat gcgacatggg gtggtgaaaa acctgtcgat
      541 gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa atccacattg
      601 aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt atcgcccaat
      661 aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga caatcttccc
      721 tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg cttataacaa
      781 ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg
      841 gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa
      901 gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa
      961 tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg gcgacggctg
     1021 ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac
     1081 tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac
     1141 ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag
     1201 aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg
     1261 ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg
     1321 agcaaacgcc attgccctgg ggcgtagcag tgtagctagc ggtactgaca gcctcgcatt
     1381 tggcagacaa tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg agaccgaagc
     1441 cgctgaaaat gcaactgcta ttggcaataa tgcgaaggca aaagggacta atagcatggc
     1501 aatggggttc ggaagccttg ccgataaagt caatactatc gcattaggaa atggcagcca
     1561 ggctctggca gataatgcaa tcgccatagg ccagggcaac aaagctgatg gcgtggatgc
     1621 catcgctctg ggtaatggta gccagtcgag aggcttaaac accattgcct taggcacagc
     1681 cagtaatgca actggtgata agagtcttgc gcttggtagt aatagcagtg ccaacggtat
     1741 taactctgtc gcgctgggcg cagattccat tgcggattta gacaataccg tctctgtcgg
     1801 caatagttca ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca agtctgacag
     1861 ttacgatgcc attaatggtt cacagcttta tgccattagc gactcggtag caaaaaaggc
     1921 ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt
     1981 taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc
     2041 tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg
     2101 ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta
     2161 acggttccca actgaaagct accaatgacg atgtcgaagc caacaccacc aatatcacca
     2221 acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac gaaactaaaa
     2281 aggcattcag tgcagctcac ggccaggata ccaccagcaa aatcaccaac gttaaagatg
     2341 ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa accaccaacg
     2401 atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt gccactaaca
     2461 ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat gcgctgaaat
     2521 gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc agcaaaataa
     2581 ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac ggctctcagc
     2641 tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact aacaccacca
     2701 acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata
     2761 aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa atcaccaata
     2821 tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc cagctttatg
     2881 acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat actgacggtg
     2941 tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac
     3001 tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt tgggatgcca
     3061 ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg atcactgatg
     3121 tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca caactccacg
     3181 gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca
     3241 ccatcactgc gccgacgtac accattgcta atgctgatta cgataatgtc ggtgatgccc
     3301 tgaatgctat cgataccact cctgacgacg ctctgctctg ggatgcggac gccggtgaaa
     3361 atggtgcatt tagcgccgct cacggaaaag ataaaactgc cagtgtaatc actaacgtcg
     3421 ctaacggtgc aatctctgct gccagcagcg acgcgattaa cggctcacaa ctctatacca
     3481 ccaataagta catcgctgat gcgctgggtg gtgacgcaga agtcaacgct gacggcacca
     3541 tcaccgcacc gacttacacc attgcgaacg ccgagtacaa caacgtcggt gacgccctgg
     3601 atgcgcttga tgataacgcc ctgctgtggg ataagactgc caatggcggt gctggagcct
     3661 acaatgccag ccatgacggt aaagccagca tcatcactaa tgtcgctaat ggcagtatta
     3721 gtgaggacag taccgatgca gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg
     3781 agcagaacac ccaaattatc aatcagctcg ctggtaacac cgacgcaacc tatatcgaag
     3841 aaaatggcgc tggtatcaac tacgttcgta ctaacgacaa cgatttagcc tttaacgatg
     3901 caagcgcctc tggtgtcggc gctacagctg taggttataa cgctgtcgcg tctggtgcca
     3961 gcagcgtagc cattggtcag aacagcagca gcaccgttga taccggtatt gcgctgggta
     4021 gcagctccgt ttccagccgt gtgattgcca aaggttctcg tgacactagc gtaacggaaa
     4081 atggcgtggt gattggttat gacaccactg acggcgaact gctaggtgca ttgtcaattg
     4141 gtgatgacgg taaataccgc caaatcatca acgtagctga tggttcagaa gcccatgacg
     4201 ccgttacggt tcgccagttg cagaacgcta ttggagcggt cgccactacg ccaaccaagt
     4261 actttcacgc caactcaacg gcagaagact cactggccgt tggtgaagac tcgctggcaa
     4321 tgggtgcgaa aactgtcgtt aatggtaatg caggtattgg tatcggtttg aacactctgg
     4381 ttctggctga tgcgatcaac ggcattgcta tcggcagtaa cgcacgcgca aaccatgcaa
     4441 acagtatcgc aatgggtaac ggttctcaga ccacccgtgg tgcacagact ggctacaccg
     4501 cctacaacat ggacgcaccg cagaactctg taggtgagtt ttctgtcggt agtgaagacg
     4561 gtcaacgtca gatcacaaac gtcgcagctg gttcagcgga taccgatgcg gttaacgtgg
     4621 gtcagttgaa agtcactgat gagcgcgtag cgcaaaatac ccagagcatt actaacctga
     4681 acaatcaggt cactaatctg gatactcgcg ttactaatat cgaaaacggt attggcgaca
     4741 ttgtcaccac cggtagcacc aagtacttca agaccaacac cgatggcgta gatgccaacg
     4801 cccagggtaa agatagcgtt gctattggtt ctggttccat tgctgccgct gacaacagcg
     4861 tcgcactggg taccggttcc gttgcagagg aagaaaatac aatctctgta ggttcttcta
     4921 ctaaccaacg ccgtatcacc aacgtagctg ccagtgttaa tgccaccgat gcggttaacg
     4981 tgtcgcagct gaaatcttct gaagcaggcg gagtgcgcta cgacaccaaa gctgatggtt
     5041 ctatcgacta tagcaatatc accctcggtg gtggcaacgg cagtacgact cgtatcagca
     5101 acgtctccgc tggcgtcaac aacaacgacg cggtgaacta cgcgcagttg aagcaaagcg
     5161 cgcaggaaac gaagcaatac accgatcagc ggatggttga gatggataac aaactgtcta
     5221 aaactgaaag caagttgagc ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc
     5281 aggcttatac accgggtgcc agcatggctt ctattggtgg cggtacttac aacggtgaat
     5341 cggcagttgc tttaggtgta tcgatggtga gcgccaatgg tcgttgggtc tacaaattac
     5401 aaggtagtac caatagccag ggtgaatact ccgccgcact cggtgccggt attcagtggt
     5461 aatgatccat taagttagtg tgactaaggg caaacgccaa attgcctgat gcgttacgcg
     5521 catcaggcaa gataaggcgc tccgggtcaa caatctaagg gcaattctct gatgaggatt
     5581 gcccttttct ttaccagaca tctcccccca caagaattgg ccctaccaat tcttcgctta
     5641 tctgacctct ggttcacaat tccccaatta aaactcacat caatattgcc aatacataac
     5701 atttagttaa ccattcattg tcattatccc tacacaacac aattggcagt gccactttta
     5761 cacaacgtgt gacaaggaga tgagcaacag actcattaca cgatgtgcgt ggactctagg
     5821 agacctgcaa tgaatctctg gcaacaaaac tacgatcccg ccgggaatat ctggctttcc
     5881 agtctgatag catcgcttcc catcctgttt ttcttctttg cgctgattaa gctcaaactg
     5941 aaaggatacg tcgccgcctc atggacggta gcaatcgccc ttgccgtggc tttgctgttc
     6001 tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt atggcttctt ctatggtttg
     6061 tggcccatcg cgtggatcat tattgcagca gtgttcgtct ataagatctc ggtgaaaacc
     6121 gggcagtttg acatcattcg ctcgtctatt ctttcgataa cccctgacca gcgtctgcaa
     6181 atgctgatcg tcggtttctg tttcggcgct ttccttgaag gagccgcagg ctttggcgca
     6241 ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt ttaaaccgct gtacgccgcc
     6301 ggactgtgcc tgattgttaa caccgcgcca gtggcatttg gcgcgatggg cattccaatc
     6361 ctggttgccg gacaagtaac aggcatcgac agctttgaga ttggccagat ggtggggcgt
     6421 cagctaccgt ttatgaccat tatcgtgctg ttctggatca tggcgattat ggacggctgg
     6481 cgcggtatca aagagacgtg gcctgcggtc gtggttgcag gcggctcgtt tgccatcgct
     6541 cagtacctta gctctaactt cattgggccg gagctgccgg acattatctc ttcgctggta
     6601 tcactgctct gcctgacgct gttcctcaaa cgctggcaac cagttcgcgt attccgcttc
     6661 ggtgacttgg gggcgtcaca ggttgatatg acgctggccc acaccggtta caccgcgggt
     6721 caggtgttac gtgcctggac accgttcctg ttcctgaccg ctaccgtaac actgtggagc
     6781 atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc tgtatgagtg ggtgatcaac
     6841 attccggtgc cgtacctcaa taaactggtt gcccgtatgc caccagtggt cagcgaggct
     6901 acagcctatg ccgccgtgtt taagtttgac tggttctctg ccaccggcac cgccattctg
     6961 tttgctgcac tgctctcgat tgtctggctg aagatgaaac cgtctgacgc tatcagcacc
     7021 ttcggcagca cgctgaaaga actggctcta cccatctact ccatcggcat ggtgctggcg
     7081 ttcgccttta tctcgaacta ttccggactg tcatcaacac tggcactggc actggcgcac
     7141 accggtcatg cattcacctt cttctcgccg ttcctcggct ggctgggggt attcctgacc
     7201 gggtcggata cctcatctaa cgccctgttc gccgcgctgc aagccaccgc agcacaacaa
     7261 attggcgtct ctgatctgtt gctggttgcc gccaatacca ccggtggcgt caccggtaag
     7321 atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg taggcctggt gggcaaagag
     7381 tctgatttgt tccgctttac tgtcaaacac agcctgatct tcacctgtat ggtgggcgtg
     7441 atcaccacgc ttcaggctta tgtcttaacg tggatgattc cttaatgatt gttttaccca
     7501 gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct gattgatgaa aaaaacctgg
     7561 aagcgggcat gaagttgccc gctgagcgcc aactggcgat gcaactcggc gtatcacgta
     7621 attcactacg cgaggcgctg gcaaaactgg tgagcgaagg cgtgctgctc agtcgacgcg
     7681 gcggcgggac gtttattcgc tggcgtcatg acacatggtc ggagcaaaac atcgtccagc
     7741 cgctaaaaac gctgatggcc gatgatccgg attacagttt cgatattctg gaagcccgct
     7801 acgccattga agccagcacc gcatggcatg cggcaatgcg cgccacacct ggcgacaaag
     7861 aaaagattca gctttgcttt gaagcaacgc taagtgaaga cccggatatc gcctcacaag
     7921 cggacgttcg ttttcatctg gcgattgccg aagcctcaca taacatcgtg ctgctgcaaa
     7981 ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa gcatagccgt cagcggatgt
     8041 atctggtgcc accggttttt tcacaactga ccgaacaaca tcaggctgtc attgacgcca
     8101 tttttgctgg tgatgccgac ggggcgcgta aagcaatgat ggcgcacctt agttttgttc
     8161 acaccaccat gaaacgattc gatgaagatc aggctcgcca cgcacggatt acccgcctgc
     8221 ccggtgagca taatgagcat tcgagggaga aaaacgcatg attatttccg cagccagcga
     8281 ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg ttccactata tggatggtgg
     8341 tgcatattct gaatacacgc tgcgccgcaa cgtggaagat ttgtcagaag tggcactgcg
     8401 ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa acgacgctgt ttaatgagaa
     8461 attgtcgatg ccggtagcac tggcaccggt gggtttgtgt ggcatgtatg cgcgtcgtgg
     8521 cgaagttcag gcagccaaag cggcggacgc gcatggcatt ccgtttactc tctcgacggt
     8581 ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag cgcccaatgt ggttccagct
     8641 ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg gagcgagcaa aagcagcggg
     8701 ttgttcgacg ctggttttca ccgtggatat gccgacaccg ggcgcacgct accgtgatgc
     8761 gcattcaggt atgagcggcc caaatgcggc aatgcgccgc tacttgcagg cggtgacgca
     8821 tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtcca catgatttag gtaatatctc
     8881 agcttatctc ggcaaaccga ccggactgga agattacatc ggctggctgg ggaataactt
     8941 cgatccgtcc atctcatgga aagaccttga gtggatccgc gatttctggg atggcccgat
     9001 ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat gcagtacgtt ttggtgctga
     9061 tggaattgtg gtttctaacc acggtggccg ccagctggac ggtgtactct cttccgctcg
     9121 tgcgttgccc gctattgcag atgcggtgaa aggtgatatc gccattctgg cggatagcgg
     9181 aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc ggtgccgaca ccgtactgct
     9241 gggtcgtgct ttcctgtatg cgctggcaac agcgggccag gcgggtgtag ctaacctgct
     9301 aaatctgatc gaaaaagaga tgaaagtggc gatgacgctg actggcgcga aatcgatcag
     9361 tgaaattacg caagattcgc tggtgcaggg gctgggtaaa gagttgccta cggcactggc
     9421 tccgatggcg aaagggaatg cggcatagtc gtttgcgccc cctcacccta accctctccc
     9481 tcagggagag gggaccgttc ggcgctgtat gtactccctc actctgaaac gacaccgcac
     9541 tctttttttc tccctcgccc ctccggggag ggccggggtg aggggaaaag gccgcacggt
     9601 tcaccaattt tctgctatcc tgcccccgca ctaagggggc agtatgctaa acatcgtact
     9661 ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg ctaataccgg
     9721 ctttcgtcta catatcatcg aaccgatggg atttgcctgg gacgacaagc gcctgcgccg
     9781 cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact atcgcgcgtt
     9841 cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta cgcctgctca
     9901 cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa cacgcggcct
     9961 gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgcattc cgatggtgcc
    10021 ggacagccgc agcatgaatc tgtccaatgc ggtgtcggta gtggtgtatg aagcctggcg
    10081 gcagctggga tatccgggag cgttgttgag agattagtta ctgtatgccg gatagatgat
    10141 tacatcgcat ccggcacgat cacaggacat tagatcccat ccccatactc aaatgtatgg
    10201 ttaataccgt tgaaatgctg gtccatatcc attgatggct tatcgctgtc tggtttaccg
    10261 acaatacgag ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg cagcaccacg
    10321 gaacctgcgc caatcttcgc gccgcaacca acttcaatat tgccgaggat tttcgcgccc
    10381 gcgccaatca tcacaccttc acgaattttc gggtgacggt caccaccaga tttacccgta
    10441 ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caattaccgc cgtttcaccc
    10501 acaacgatgc ccgtggcgtg gtcgagcata atgccacggc caatttttgc tgccgggtga
    10561 atatcgacct ggaacgtcac agaaacctgg ttttgcagaa agattgccaa tgcgcgacgc
    10621 ccctgattcc acaaccagtg accgatgcga taggcctgca aggcatgaaa acccttcagg
    10681 tataacaacg gggttgagta tttatcgact gccgggtcgc gggtacgcac cgcctgaata
    10741 tcacaggccg cagaggcgat catttccggg tcagcggcgt aggcttcttc aaccacttca
    10801 cggatagcaa tagcaggcat aattggcgat gacagcttgt tcgccagcat gtagctcagt
    10861 gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc
    10921 tcacagtccg ccagcgttct ggcttcggct ttaatattgt tccagacaat ttccagttct
    10981 tcacacgaca ttgcttactc cacacgatga gataatgacc ggcccgttct gcgcgggccg
    11041 ggtcatagcg gtaacaaagg ttccctgggg ttagtgactg ctgcgctcgt ccttgcgtgc
    11101 acgacctaat aatgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata
    11161 aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt
    11221 cgtattgcgg tagccttcca ccacctgacc aatcttctcc tgcgcgcttt gtacatccat
    11281 gccctgaccg agcatcatac caaaacggcg gttacgcgac tggttgtcgg tacaggtaag
    11341 caccagatcg cccagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc
    11401 accaagacgc gacatttcag ccagcccacg ggtgatcagc gccgtacgcg cattcgcacc
    11461 aaaaccgata ccgtcagaca tccccgcgcc aatggcaatg acgtttttca ccgcgccgcc
    11521 aagctgcacg ccaatgaaat ccgggttgct gtaaacgcgg aaacttttgc cgcagtgcag
    11581 cagctgctgg agatcatcgg caaaggtctg atcggtcgag gccagcgaaa tagctgtcgg
    11641 taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca gcggaatttg
    11701 atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttccg cttccagccc
    11761 tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct ggcgcagcac
    11821 ttcaccaaag acatggctgg gtacgacgac gagaatatta cgactggctg ccagcgcagt
    11881 ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc
    11941 gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac
    12001 ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc cggcaccgat
    12061 cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg
    12121 ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc
    12181 agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac
    12241 agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg
    12301 atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccaaggag
    12361 gccgttacgg taacacgcag taccacttcg tatacgtcat ctgccagttg ggaagatgcc
    12421 gtatccagat caagtttaac ttctggttgc caatctttct ggaaaacgtg cggcgcgttc
    12481 ggcgcttcga aagagatatc cttggtataa atacgttgga tctggaaagt catttcagtg
    12541 ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg
    12601 taataccaac gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg
    12661 catacaagtc atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg
    12721 tgcaaccgct gcgtttgatc atctcttcac gcttggcggc attgccatcg atcggcaact
    12781 cctggaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg
    12841 tttctttggt atagatttca acattggcca taatttagct cctgtttttt taccctgaaa
    12901 tttcatgttg cagggcggct gcaaaatgga gtgtccccag aagcttataa caataagtcg
    12961 ctgggggcct aattgcagct aacggcctgc atcatgaaag acgacaggta aattatttgc
    13021 cgcgcaccag aggcaagttt tcgccagccc agccagcaac gccctctttc agcacgaata
    13081 cttgcgcaaa accagctttc gtcagcgcgt ttgcaggctc ctggcactgc atgccagaac
    13141 cgtctaccac gataaccgat ttgtctttgt gcttctcaag ctcaccaaca ttgttggctt
    13201 tgatttcgct cggcaacaga ttgatagaac ctgcgatatg gcctttacgg aagtcatcac
    13261 gctgacgtaa atccacaacc acagcgtctt ctttgttgat cagacgcgta gcttcaccac
    13321 gagtaatcac cttcactttc gaggtcaggc tcttaaacgt agtcacaaga accgccacca
    13381 gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc aacaaattgc ataatttctt
    13441 gcatgggggg taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg agtatacctg
    13501 cgcggtgcgg caaatacagc cagcgcgtta actggaatgc aattttgcgg ggcacgacga
    13561 aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt cgtatatttt gatcttcaga
    13621 ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt gagtatgagg
    13681 ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt gattctggat ggctatggct
    13741 atcgtgaaga acagcaggat aacgccattt ttagtgctaa aaccccggta atggatgcac
    13801 tgtgggccaa tcgtccgcat accctgatcg acgcttccgg tctggaagtc ggtctgcctg
    13861 accgtcagat gggtaactcc gaagtaggcc acgttaacct gggtgccggc cgcatcgtgt
    13921 atcaggacct gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt gctaatccag
    13981 tgctggctgg cgcggtacat aaagcgaaaa acgcaggtaa agcagtacac attatgggtc
    14041 tgctctccgc aggcggcgta cacagccacg aagatcacat catggcgatg gtagaactgg
    14101 cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt cgcgacaccc
    14161 cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct gcgctgggta
    14221 aaggccgcgt agcgtccatc attggtcgtt actacgcgat ggaccgcgat aaccgttggg
    14281 atcgcgttga aaaagcctat gacctgctga ctctggcgca gggtgagttc caggccgata
    14341 ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga aaacgacgag ttcgtgaaag
    14401 cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc gatgcgctga
    14461 ttttcatgaa cttccgtgct gaccgcgcgc gtgaaatcac tcgtgcattc gtgaacgctg
    14521 atttcgatgg cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg ctgaccgaat
    14581 acgccgctga catcaaaact gcggttgctt acccacccgc ttccctggtt aacaccttcg
    14641 gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc gaaaaatatg
    14701 cccacgttac tttcttcttc aacggtggcg tagaagagtc gttcaaaggc gaagatcgca
    14761 ttctgatcaa ctcaccgaaa gtggctacct acgatctgca accggaaatg agctccgcag
    14821 agctgaccga aaaactggtt gcggccatca agagcggcaa atacgacacc attatctgta
    14881 actatccgaa cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg gttaaagcgg
    14941 ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc gtgggtggac
    15001 aactgctgat caccgctgac cacggtaacg ctgaacagat gcgcgatccg gcaacgggtc
    15061 aggcacacac ggcacacacc aacctgccag ttccgctgat ttacgttggt gataagaacg
    15121 tgaaagcggt tgcaggcggc aaactttctg acatcgcgcc gaccatgttg tcgctgatgg
    15181 gtatggaaat cccgcaagag atgactggta agccgctgtt catcgtggaa taatccctcc
    15241 ccatgagggg aaaggcgatt aataccatga cacgggccgt gaaaccgcgc aggtttgcaa
    15301 tcaggcccat catctacgcc agcgttctta gcgctggcgt attgttgtgc gccttttccg
    15361 cccacgcgga tgagcgtgac caactcaaat ctattcaggc cgatatcgcc gcaaaagagc
    15421 gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct cgcacaattg aaaaagcagg
    15481 aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg ctcaatcaac
    15541 tcaataaaca gattgatgag atgaacgcgt cgattgccaa actggagcag caaaaagccg
    15601 cccaggagcg cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc gagcataccg
    15661 gtattcagct aattctcagc ggtgaagaaa gccagcgtgg acagcgttta caggcttatt
    15721 tcggctatct caaccaggcg cgacaagaaa ccattgccca gttgaagcaa acgcgtgaag
    15781 aagtcaccat gcagcatgct gaactggaag agaaacaaag cgagcaacaa acgctgttat
    15841 atgaacagcg cgcccaacag gcgaaactga ctcaggctct gaacgagcgt aaaaagacgc
    15901 tggcggggct ggaatcttcc atccagcaag gtcagcagca gctaagtgaa ctgcgtgcca
    15961 acgaatcccg tctgcgtaac agcattgccc gtgctgaagc cgcggcgaaa gcgcgtgctg
    16021 aacgagaagc gcgtgaggcc caggcggttc gcgagcgcca gaaagaagcg acgcgcaaag
    16081 gcaccaccta caaaccgacc gaaagcgaaa aatcgctgat gtcccgaact ggtggcctgg
    16141 gtgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat cgctatggcg
    16201 agcagctaca gggtgaatta cgctggaaag ggatggttat cggtgcctct gaaggtactg
    16261 aagttaaagc aattgccgac ggtcgagtga ttctggctga ctggctgcaa ggttacggtc
    16321 tggtggtggt ggttgagcac ggtaaaggcg acatgagtct ttacggctat aatcagagcg
    16381 cactggtgag cgttggttcg caggttcgcg cgggccagcc aattgcactg gtgggcagca
    16441 gtggcggtca gggtcggcct tcactctatt tcgaaattcg ccgccagggt caggcggtca
    16501 atccacagcc gtggttggga agataagttt tgtttccatt tcgtcgtaac gttcttgcat
    16561 ttgccgctct gttggcgctc tcctcccccg tacttgctgg caaacttgcc atcgtcattg
    16621 atgattttgg gtatcgcccg cacaacgaaa accaggtgct ggcgatgcct tccgctatct
    16681 ccgtcgctgt attacccgat tcaccgcacg ccagagagat ggcgaccaaa gcgcataaca
    16741 gcggtcacga agtgttgatt catctcccga tggcaccgtt gagtaaacag ccgctggaga
    16801 aaaatacgct acgcccggag atgagcagcg acgaaattga gcgcattatt cgtagtgcgg
    16861 tcaataacgt gccctatgcc gtggggatca acaaccacat gggcagcaag atgacctcta
    16921 acctgtttgg tatgcagaaa gtgatgcagg cgctggagcg ttacaatctt tacttcctcg
    16981 acagcgtaac catcggtaat acccaggcga tgcgcgccgc gcaaggtact ggcgtgaagg
    17041 tgatcaaacg gaaggtgttc ctcgacgatt cgcagaacga agcggacatc cgtgtgcaat
    17101 ttaatcgcgc aattgatctg gcgcgtcgca acggttcaac cattgccatt gggcatcctc
    17161 acccttcaac ggtacgcgtg ctacaacaga tggtttataa cctgccgcca gacattacgc
    17221 tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga tacttctaca ccgccgaaaa
    17281 acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc aaaccgaaga
    17341 aaccgataga acctgtttat gctaatcgct tctttgaagt attaagcgaa agcatcagcc
    17401 agagcacgct gatcgtttac ttccagcatc agtggcaagg ctggggcaaa cagcccgaag
    17461 cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat ggtgcgaccg
    17521 cgttttgtcg cgccattgcc ataagcggaa agaccacagt aacgcctggt aaccgacctt
    17581 cacgctgcgc acattggtaa tcaggcgctt atacataccc ggaagtaaat atctcggcaa
    17641 tcatccgttg gcgggtaaga atatccggct ctttgcg
//
LOCUS       DAZPDE010000080        17643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPDE010000080 DAZPDE010000000
VERSION     DAZPDE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.080"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHU0J_002345"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHU0J_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHU0J_002346"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHU0J_002346"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1217017.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHU0J_002347"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACHU0J_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHU0J_002348"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACHU0J_002348"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1217018.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHU0J_002349"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACHU0J_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1217019.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="ACHU0J_002350"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="ACHU0J_002350"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1217020.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="ACHU0J_002351"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="ACHU0J_002351"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1217021.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHU0J_002352"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACHU0J_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1217022.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHU0J_002353"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACHU0J_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1217023.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHU0J_002354"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACHU0J_002354"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1217024.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHU0J_002355"
                     /pseudo
     CDS             complement(8149..9470)
                     /gene="astB"
                     /locus_tag="ACHU0J_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHU0J_002356"
     CDS             complement(9467..10945)
                     /gene="astD"
                     /locus_tag="ACHU0J_002356"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR1217025.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHU0J_002357"
     CDS             complement(10942..11976)
                     /gene="astA"
                     /locus_tag="ACHU0J_002357"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1217026.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHU0J_002358"
                     /pseudo
     CDS             complement(11973..13193)
                     /gene="astC"
                     /locus_tag="ACHU0J_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13639..14445
                     /gene="xthA"
                     /locus_tag="ACHU0J_002359"
     CDS             13639..14445
                     /gene="xthA"
                     /locus_tag="ACHU0J_002359"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1217027.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14612..15322
                     /locus_tag="ACHU0J_002360"
     CDS             14612..15322
                     /locus_tag="ACHU0J_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1217028.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHU0J_002361"
     CDS             15327..16004
                     /gene="ydjY"
                     /locus_tag="ACHU0J_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1217029.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16019..16726
                     /locus_tag="ACHU0J_002362"
     CDS             16019..16726
                     /locus_tag="ACHU0J_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1217030.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16726..17274
                     /locus_tag="ACHU0J_002363"
     CDS             16726..17274
                     /locus_tag="ACHU0J_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1217031.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17284..17610
                     /locus_tag="ACHU0J_002364"
                     /pseudo
     CDS             17284..17610
                     /locus_tag="ACHU0J_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4365 a         4685 c         4308 g         4285 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc ctccctcgcg
     8161 ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg
     8221 cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta
     8281 acgatccacc cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt
     8341 caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc
     8401 gccattcgcc atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc
     8461 ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag
     8521 caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga
     8581 caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac
     8641 ccgcgtgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     8701 cggttactca cggcaatcac gtcattatga aaaacgctct ggtcgataac gtccgggttt
     8761 tgctgggcga aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg
     8821 gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
     8881 ccgtagacaa aaagtagcat acccggttca ccgtaatgac cgccgagacg attgtggttt
     8941 gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc
     9001 tcttcgtcct taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg
     9061 tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca
     9121 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa
     9181 agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat
     9241 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcggaatcac ggcctggggg
     9301 aatcccgcat cggcaagggc tttcattttc agtaagccct gttttgccgc cagtcgcggg
     9361 ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa
     9421 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     9481 catcggaaaa atccagcccg gggttaagcg tggcgggcaa tgttaacgag tccgactcca
     9541 ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     9601 cggaagcacc aatgccgccg aatggggcgg tactggcagc accagtaagc ggtttgttcc
     9661 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     9721 aaaccagacc gcaaaagagt ccgaagcgag tgttgttcgc catccgaatc gcctcatcaa
     9781 aagtatcata acgccagacg cgcaaaaacg gcccgaacac ctcttcatcc ggcacaccgc
     9841 caacgcccgt catttcaatg atccctggtg tcagcaacga tgtccctgct tgtaataagc
     9901 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
     9961 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    10021 tgcccggcgt taatcgctgg ctgacggcaa ccagactagc aagaaacgca tcgccctgcg
    10081 ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg
    10141 ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg
    10201 gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    10261 gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg
    10321 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    10381 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    10441 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    10501 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    10561 tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa
    10621 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    10681 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
    10741 caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg
    10801 ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc
    10861 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc
    10921 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    10981 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    11041 ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc
    11101 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    11161 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa
    11221 gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    11281 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata
    11341 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    11401 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    11461 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    11521 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    11581 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    11641 aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt
    11701 gccgacgcga tagttgtacc agggatcgtt cagcccaacc gccacctcaa tagcgcaaat
    11761 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt
    11821 gggcagttca ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    11881 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    11941 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
    12001 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    12061 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    12121 gcttccagag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc
    12181 aacaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat agtattaaga
    12241 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg
    12301 agctccagca ctttgcctgc taccgccgag gccagcggat taccgccata ggtggtgcca
    12361 tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc accgaccggg
    12421 aaaccgccgc ccagcgcttt ggcggtggtt aacagatcgg gcgtcacgcc gtagtgcata
    12481 taggcataca gctcaccagt gcgcccgacg ccggtttgta cctcatcaaa aatcagcaag
    12541 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc
    12601 acaccgcctt cgccctagat gggctcgaca atcaccgcac aggtggcgtc gtcaatcagc
    12661 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg cagtggcgca
    12721 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    12781 aacgcatttt tgaacgccac gatgccactc ttatggctgc cgtagcggtc gtgagcgaat
    12841 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
    12901 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
    12961 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
    13021 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    13081 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
    13141 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    13201 tctacagtaa ataattcgtt atttgtatgt taacaataag taatgtttgc gctgtaaatg
    13261 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggctaactgg
    13321 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    13381 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    13441 ctagaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
    13501 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    13561 cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca ctctttatct
    13621 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    13681 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    13741 acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg
    13801 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    13861 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat tattatggcg
    13921 gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    13981 agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa tctgcaaaac
    14041 tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga tatgaatatc
    14101 agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc
    14161 ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg
    14221 gtggtcgata ccttccgcca tgctaatccg caaacagcag atcgtttctc atggtttgat
    14281 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
    14341 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa
    14401 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    14461 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    14521 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    14581 tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaact ttctgtttgc
    14641 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
    14701 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    14761 ctatattctg ttattcatca atgcccccct ctttctgtta ccaggaagca tactggtgat
    14821 cgctggagga atagtttttg gcccgctctt agggacacaa ctctcattaa ttgccgcaac
    14881 gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt tactgctgaa
    14941 atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat
    15001 tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc
    15061 ttacggattc accaccatcg ccttctggcc ttataccctt atttcggcac tcacgaccct
    15121 gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
    15181 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctca tccagctcgc
    15241 aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca gcccacttac
    15301 tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag
    15361 gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga
    15421 atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
    15481 ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    15541 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatggctt
    15601 atgcgccccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa
    15661 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
    15721 tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg
    15781 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    15841 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    15901 atggtgcggt ggaaaaacac ggcgaagtga aattcaaagg caatgcctca gttctcccgg
    15961 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccaa gataaaggat
    16021 gatgaagata caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
    16081 cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    16141 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    16201 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    16261 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
    16321 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    16381 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg acggcttttt
    16441 cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga
    16501 tccaatcagc tatgctgccg gtctgacttc aatacgtttt cgctcgtttt ttatcgccac
    16561 cgggcttggt caattaccgg cgactattgt ttattcctgg gcaggcagca tgttaacagg
    16621 cggtactttc tggtttgtca ccgaactgtt tattctgttt gccctgaccg tggtgatttt
    16681 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gactgatggg tttaccgccg
    16741 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    16801 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tttggtgtca
    16861 atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt
    16921 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat caccagtatg
    16981 aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa
    17041 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc
    17101 acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    17161 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    17221 gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggaggg
    17281 aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    17341 gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg gtttaacgcc
    17401 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    17461 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc
    17521 attcagaccg aagctgctgc cggtcgtaaa acgggcggct cggtggatct gctctgggtg
    17581 aacggcgaaa acttccgcac cttaaaagag taatgccagt cagttaagca actgactggc
    17641 att
//
LOCUS       DAZPDE010000081        17186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPDE010000081 DAZPDE010000000
VERSION     DAZPDE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.081"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            52..614
                     /gene="srlA"
                     /locus_tag="ACHU0J_002365"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             52..614
                     /gene="srlA"
                     /locus_tag="ACHU0J_002365"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            611..1570
                     /gene="srlE"
                     /locus_tag="ACHU0J_002366"
                     /gene_synonym="gutE"
     CDS             611..1570
                     /gene="srlE"
                     /locus_tag="ACHU0J_002366"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1217032.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1581..1952
                     /gene="srlB"
                     /locus_tag="ACHU0J_002367"
                     /gene_synonym="gutB"
     CDS             1581..1952
                     /gene="srlB"
                     /locus_tag="ACHU0J_002367"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1217033.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1956..2735
                     /gene="srlD"
                     /locus_tag="ACHU0J_002368"
     CDS             1956..2735
                     /gene="srlD"
                     /locus_tag="ACHU0J_002368"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR1217034.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2840..3199
                     /gene="gutM"
                     /locus_tag="ACHU0J_002369"
     CDS             2840..3199
                     /gene="gutM"
                     /locus_tag="ACHU0J_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1217035.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3266..4039
                     /gene="srlR"
                     /locus_tag="ACHU0J_002370"
                     /gene_synonym="gutR"
     CDS             3266..4039
                     /gene="srlR"
                     /locus_tag="ACHU0J_002370"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1217036.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4032..4997
                     /gene="gutQ"
                     /locus_tag="ACHU0J_002371"
     CDS             4032..4997
                     /gene="gutQ"
                     /locus_tag="ACHU0J_002371"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1217037.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4994..6508)
                     /gene="norR"
                     /locus_tag="ACHU0J_002372"
     CDS             complement(4994..6508)
                     /gene="norR"
                     /locus_tag="ACHU0J_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1217038.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6695..8134
                     /gene="norV"
                     /locus_tag="ACHU0J_002373"
     CDS             6695..8134
                     /gene="norV"
                     /locus_tag="ACHU0J_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1217039.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8131..9264
                     /gene="norW"
                     /locus_tag="ACHU0J_002374"
     CDS             8131..9264
                     /gene="norW"
                     /locus_tag="ACHU0J_002374"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1217040.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(9392..11644)
                     /gene="hypF"
                     /locus_tag="ACHU0J_002375"
     CDS             complement(9392..11644)
                     /gene="hypF"
                     /locus_tag="ACHU0J_002375"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1217041.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11797..12324)
                     /gene="hydN"
                     /locus_tag="ACHU0J_002376"
     CDS             complement(11797..12324)
                     /gene="hydN"
                     /locus_tag="ACHU0J_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1217042.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12473..13483)
                     /gene="ascG"
                     /locus_tag="ACHU0J_002377"
     CDS             complement(12473..13483)
                     /gene="ascG"
                     /locus_tag="ACHU0J_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1217043.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(13545..13616)
                     /locus_tag="ACHU0J_002378"
                     /pseudo
     CDS             complement(13545..13616)
                     /locus_tag="ACHU0J_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13626..15335)
                     /gene="hycE"
                     /locus_tag="ACHU0J_002379"
     CDS             complement(13626..15335)
                     /gene="hycE"
                     /locus_tag="ACHU0J_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1217044.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15353..16276)
                     /gene="hycD"
                     /locus_tag="ACHU0J_002380"
     CDS             complement(15353..16276)
                     /gene="hycD"
                     /locus_tag="ACHU0J_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1217045.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16279..>17186)
                     /locus_tag="ACHU0J_002381"
     CDS             complement(16279..>17186)
                     /locus_tag="ACHU0J_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR1217046.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         4067 a         4841 c         4538 g         3740 t
ORIGIN      
        1 agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac aatgatagaa
       61 accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg agaggtgttt
      121 accgggatgg tgaccggcat tcttccgctg ttgattagcc tgcaggttat catgaacgca
      181 ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac
      241 cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg caatccgatg
      301 accttaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta cgcggcggcc
      361 tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgc gaactgtttg
      421 tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc ccactggcgg
      481 tgagttatct gctggttggt ctggtcacca atttcttccg cggctgggtg accgatctga
      541 ccaccgccat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt cacctggcag
      601 gagcaacatc atgacgcgta ttcggatcga aaaaggaacg ggtggctggg gcggcccgct
      661 tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc accgccggta cccggcctgc
      721 gattgttgac aaactggcac aacttactgg ctggcaggct attgacggat ttaaagaagg
      781 tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg
      841 cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg gcaagtccgg
      901 tccgctggcg cagtacattg tggaagatat ttatgtctct ggcgtaaaag aagaaaacat
      961 cactgtagtg ggtgatgcga caccacaacc ctcttccgtg ggccgtgact atgacaccag
     1021 taagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg gcatggggtc
     1081 cgccgttgcc gtgttgtttc agtctggtcg tgacaccatc gacactgtat taaaaaccat
     1141 tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt ctggccttgg
     1201 tgactggatt gcccacggtc ttgctccgct agcgagccat ccactgggtc tggtcatgct
     1261 ggcgctcatc tgctccttcc cactgctttc acctttcctc ggcccaggcg cagttatcgc
     1321 acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc cgccgcatct
     1381 ggctttaccg gcactgtttg ccatcaacgc gcaggcggct tgcgacttca tcccggtcgg
     1441 tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt
     1501 gagccgcttt ttaaccggcg cgccgactgt actgatcgcc tggtttgtct ccggttttat
     1561 ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc cgtatcggcg
     1621 cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag
     1681 cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt gcactccatc
     1741 ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg
     1801 cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag
     1861 cggaatttcc gggcactgtc catgtggcag gccctgtacc cgacgatatc gcgccgggat
     1921 cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg
     1981 gtggtgggca aaccttaggc gcgttcctgt gccacggtct ggctgccgag gggtatcgcg
     2041 tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg
     2101 aatatggtga aggtatggcg tacggttttg gtgctgacgc cactagcgag caaagcgttc
     2161 tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg gtctatagcg
     2221 ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt gaccgttcgc
     2281 tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc
     2341 gcgacgggat tcagggtcgc attattcaga tcaactcgaa atccggcaaa gtgggcagca
     2401 aacacaactc tggctacagc gcagcgaaat ttggtggcgt cgggctgact caatcactgg
     2461 cgctggatct ggcggagtac ggcattacgg tgcattcact gatgctcggt aacctgctga
     2521 aatcgcggat gttccagtca ctgttgccac aatacgcgac caagctgggt atcaaaccgg
     2581 atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc gattatcaag
     2641 atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc gtcgtactgc accggacagt
     2701 cgatcaatgt caccggcggt caggtgatgt tctgatcaac agcggagatc cgttaaggat
     2761 ctccgtgaga ctatagaatg cctgatgcgc tacgctcatc aggcatacag gacttccgcc
     2821 actacattaa ggaaaagtta tggtatccgc actcatcacc gtcgccgtta tcgcctggtg
     2881 tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt aaccgtgcct tcgacacact
     2941 atgccagcaa gggcgggttg gcgtggaccg ttccagcggg cgctttaaac cgcgggtcgt
     3001 ggtcgccatc gcgctggacg atcagcagtg catcgtcgac accttgttta tgaaaggact
     3061 gactgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg cgggtgattt
     3121 acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat cattggcgct
     3181 taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt tatcattttg
     3241 aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg ccattctgga
     3301 gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact ttgacaccac
     3361 aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg tcattcgtac
     3421 ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc ataaaacgct
     3481 catcaacacc cacaagaaag agctgattgc agaagctgcc gttagtttta tccatgatgg
     3541 cgattcgatc attcttgatg ctggcagtac cgttttgcag attgttcccc tgctctcgcg
     3601 ctttaataac atcacggtga tgaccaacag cctgcatatc gtcaatgcgc tatccgaact
     3661 ggataacgaa caaactatcc tgatgccagg cggaacattt cgcaaaaaat cggcctcatt
     3721 tcacgggcaa ctggcagaga atgccttcga gcatttcacc ttcgataaat tgtttatggg
     3781 caccgacggc atcgatctca atgcgggcgt aaccacgttc aacgaggttt ataccgtcag
     3841 taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat caaagtttgg
     3901 ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctaa ttaccgacgc
     3961 aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa gggatcgatg tgatcataac
     4021 cggagagagc aatgagtgaa gcactactga acacgggacg tcagacgtta atgctggagt
     4081 tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc gccgccaata
     4141 tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg ggccacattg
     4201 gtaagaaaat cgccgcaaca cttgccagta ccggcacacc ggcttttttt gtccatccgg
     4261 cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg ctgtttatct
     4321 cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa gataaatcta
     4381 tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg gcgaaagcgg
     4441 tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg acctccagca
     4501 ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag gcgcgcggtt
     4561 ttaatgaaga agattttgcc cgctcccacc cagctggggc actgggcgct cgcttgctga
     4621 ataaagtgca tcatctgatg cgtcgtgatg acgctatccc acaggtagcg ttaaccgcca
     4681 gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct ggggctggtg gcggtatgtg
     4741 acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc tggctggtag
     4801 gcggcggcgc actcaccacg ccagttaatg aagcgatgac gacaggcggc accacattac
     4861 aggcacaaag tcgcgccatc gacgccaaag aagttctgat gaaacgcaaa atcactgccg
     4921 caccagtggt agatgaaaac ggcaaactca ccggcgcaat taacctgcag gatttctatc
     4981 aggccgggat tatttaatcc ttcaatccca gacgtttcgc cagccgatgc aggttggcga
     5041 cgtcagtttc cagcattcgc gcacaggctg cccagttgtg atgattttgt gccagtgcct
     5101 ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc ttaacaacgg
     5161 gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa tgttgcgcct
     5221 caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag cactaccgcc cgatgaatag
     5281 catgttccag ttcgcgcacg tttcccggaa aactgtagtg ttgcagtaaa tttcgcgccc
     5341 cggcacttaa taccacgcgg gagagcccct gccgcaaacg acactgctcg cagaaatacc
     5401 ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa agtggaaaca
     5461 cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc cagcacctct tcgcgtaagt
     5521 cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca tcgccaacgc
     5581 gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc aacgacaact
     5641 cgccgatctc atcgagaaac agagtaccgt tatccgccat ttcaaacttc ccgctgcggt
     5701 tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt tccgccacac
     5761 tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt ggcgaggctt
     5821 catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg atcagaacgt
     5881 tgagatcgga cgccgccaca atctcaatct cttttttcag ttgcgtcatg ccaggggaca
     5941 agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt ggcatcgcct ggcagcatat
     6001 tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc gccagcgcgg
     6061 caatcagccg taactcttcg tcgctgaaaa catcgaactg atcgggctgc atcccgtcga
     6121 gcgtcagtgc gccgatcagg ttttgtccga caaacaatgg cagaccaacg caggcgtgaa
     6181 ccttcagact ctcctgccca ggaatcaaac cgtcataggg atcgggcaat tcgctgtctg
     6241 cgggaaagcg caccacatcc ccggcgcggg caatcgcttc cagccgtgga tgcccttcca
     6301 gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc gatggcaagc ggaataaact
     6361 gccgcgaatc gtaacgtagc aacgcagacg catcgcactc cagcacctga cgtagcgtgg
     6421 tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg atggcgatat
     6481 tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga ctatagatat
     6541 tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag cataatttta
     6601 tttatagagt aaaaacaatc agataagaaa ctggcacgca atctgcaatt agcaagacat
     6661 ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga aaaataacat
     6721 tcattgggtg ggtcaacgtg actgggaagt gcgtgatttt cacggcacgg aatataaaac
     6781 gctgcgcggc agcagctaca acagctacct catccgcgaa gaaaaaaacg tgctgatcga
     6841 caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct
     6901 ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgccg gggcgctgac
     6961 cgaactgatg gcgcaaattc ccgatacgcc gatctactgt actgccaacg ctatcgactc
     7021 gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa ctggcgacac
     7081 gctggatatc ggcaacggca aacagctcat ttttgtcgaa acaccaatgc tgcactggcc
     7141 ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg
     7201 tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg agcttttcga
     7261 gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg taacgccgaa
     7321 aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct cccacggcgt
     7381 ggtctggcgc gataacccga cgcaaattgt cgagctgtac ctgaagtggg ccgctgatta
     7441 tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc gcatgatggc
     7501 tgacgctatc gcccagggaa ttgcggaaac cgacccacgc gtggcggtga aaattttcaa
     7561 cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa aaggcgtgct
     7621 ggtcggcact tcgacgatga ataacgtgat gatgccgaaa atcgccgggc tggtggagga
     7681 gatgaccgga ttacgcttcc gtaacaaacg cgccagtgct ttcggatctc acggctggag
     7741 cggcggtgcg atggatcgcc tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct
     7801 cagcctgaaa gccaaatggc gaccagacca ggacgctctg gagttatgcc gtgaacacgg
     7861 tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg tgaatacggt
     7921 agttaaagaa gaaacctctg ccacgacgac ggctgacctc ggcccacgaa tgcagtgcag
     7981 cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg ttgcgccagg
     8041 aacgccgtgg agtgaagtcc cggataactt cctctgcccg gaatgctccc tcggcaaaga
     8101 cgtctttgac gaactagcat cggaggcaaa atgagcaacg gcattgtgat catcggttcg
     8161 ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgccac tattccatta
     8221 accctgattg ccgccgacag catagatgag tacaacaaac ccgacctcag ccatgttatc
     8281 agtcaggggc aacgtgccga cgaccttacc cgccagacgg cgggtgaatt tgccgggcag
     8341 tttaatctgc gcctgtttcc gcacacctgg gtgacggata tcgatgccga agcccatgtg
     8401 gtgaaaagcc agaataatca gtggcaatac gacaagttag tgctggcaac cggtgccagc
     8461 gcctttgtcc cgcctgtgcc tgggcgtgag ttaatgctga cgttaaatag tcagcaagag
     8521 tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg tgttgattgt cggcggtggt
     8581 ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt cacgctaatc
     8641 gacaacgctg ccagtattct ggcgtcatta atgccaccag aagtaagcag ccgcttgcag
     8701 catcggttga cggagatggg cgttcatctg ctgttgaaat ctcagttgca ggggctggaa
     8761 aaaacggatt ctggcattct ggcaacgctg gaccaccagc gtagtatcga agtggatgcg
     8821 gtaattgccg ccaccggact gcgcccggaa acagccctgg cacgacgcgc cgggctgacg
     8881 attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga tatttacgcg
     8941 ctgggcgatt gcgcggaaat taacggtcag gtattgccgt tcctgcagcc gattcaactt
     9001 agcgggatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa actaccggcg
     9061 atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga aacccagcgt
     9121 caggatttac gctggcagat taataccgaa cgccagggaa tggtggcgcg cggcgttgac
     9181 gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga ggcatttgta
     9241 ttgttgaaaa cgttgccggt gtaggtgggc tactgtgcct aaaatgtcgg atgcgacgct
     9301 ggcgcgtctt atccgaccta cggggacgca tgtgtaggcc ggataaggca tttacgccgc
     9361 atccggcaag ggtgtccaaa tgcaacacgc tttatccgtt ctggacttca cccgctaacc
     9421 aacgcgccgc agcaataacc ccctgcccca gagacaaacc gccatcaccc gccggtaaac
     9481 tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc cagacgtgca cgcagcaaac
     9541 ggttatgaat aaccccgccg ctaaatacca gcgtagtgat accacgcatc gtggcctgct
     9601 cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat
     9661 taaccagtgc ctgccagttc agccactgct gccagaaagt tgcgagatcc agttgattgt
     9721 ccacccgcgg catcgtcacc ggatgcgtca ctccgtcgca tgaggctgcg agcgcctcca
     9781 gagcacaagc cgcttcacct tcataactta acgtggctgg cgcacagccc agtgccgccg
     9841 ccactgcatc gaacaaccgc ccacacgatg atgccagcgg cgcgttaatt ccacgctcaa
     9901 tggcccgcgc cagtacgctc cagttttgct gttgcacact tgccgtttca gggtaattct
     9961 gccactccgg cacaaagcgc aggcactgcg ccagcaggtt tcgccacggc tgcttcgccg
    10021 ccaaatcgcc acccggaagc gccactgcag gcaagccgcc caggtgctca cattcgcgat
    10081 agttcacccg caggcactcg ccgccccaca aagcgccatt ctcccccata ccgataccgt
    10141 cgagcgtcaa agcaatgaca tcaccgccat ccagcggcca ctgatgctct gccagacacg
    10201 ccgctgcatg ggcatgatga tgcagtaccg tttgcgtcgg cagattcatt tcgctcgccc
    10261 actggctgga gacatagccc ggatgcacat catgcacaat gtattgcgga gtgaaatcgt
    10321 agatgttttg catcaggcgt aacgcttcgc gccactgcat ctggatgcca tcgtcactta
    10381 aatcgcccag atgctgactc aacaccgctt gttcaccgcg caccaggcag aaggtatttt
    10441 tcagatccgc gccaagacac agcacaggcg gaacattttt aaagcccgga ggcaaaacca
    10501 gcgcatccgg cacatacccc cgcgaacggc gcagcatttc gccgctttcg cgcaccactg
    10561 aatcatccat ccgctgcacg atgtcgcggt tatgtagcaa gaatccgtcg gcaatgccct
    10621 gcaaatccgc cagcgcctgt tcgttgctga tagctggtgg tttaccgctc aggttgccgg
    10681 aggtcatcac cagcgggcat tgcagttcct gtaacagcaa atgctggagc gggttcgcag
    10741 gcaacatcac cccgacttcg ttaaggccag gggcgatatc atcacaaagc tcaggtacgt
    10801 attttttatc caccagcaca atcggcgcag cgggcgtggt aagcaactgg cgcgcagcgt
    10861 ctggtaaact ttctgccact ggcaacatga ctgccagcgg tttcgccggg cgatgtttgc
    10921 gctcccgcag tgtcgccacc gcgttactgt tacgtgcatc gcaggcaaga tgaaatccgc
    10981 caatcccttt gatggcgaca atgttgccca tttttaactg tgcgatagct gcctgtaatg
    11041 ccgcctcttg ttctgcatgt tcaccatgac ttacccattc aagatgcggg ccacactccg
    11101 ggcaggccac cggctgggcg tggaagcgac gatcgagcgg gtcgcggtac tctttgtcac
    11161 aggccggaca tagcggaaac gccgccatca cggtaaacgg gcggtcgtaa ggcatcgcgc
    11221 gaataatggt gaaacgcggg ccgcagtggg tacagttgat aaacggataa cgataacgcc
    11281 gctcgcctgg ggtattcatt tcggcaaggc aagcagggca agtggcggca tccgggacaa
    11341 tttgcgtatt catggtgccg ccggtgctct ggcggatggt gaactcggtg ggcagttgtg
    11401 accagataaa cggctcacgc tcgacgctat caatacgcgc cagtggcgga cagtgctgat
    11461 gcaattgaac aagaaacgtt tccgggtctt ccagcagccg gacttctacg ccatcgccgt
    11521 cattacatac atcgccgtga agatttaatt gctgtgccag ctgccagaca aacggacgaa
    11581 aaccgacgcc ctgcactttg ccacgaatac gcagttggac accgcaagat gtgttttttg
    11641 ccattgagtt attcccgcca tcatgaattg cgtaacccgc cctgccggac acgacagcgt
    11701 cgtatccggc agtctcaggt cggcgatacc gcccgctccg tattctacga atatttccgg
    11761 gaattccttt gatgccagaa cagttctgta agatttttag aacatcagcg ccgtacggcg
    11821 gcgtttttct gcgctcagtt gttcaagttt attacgatcg acacaaatca gcgcatgggt
    11881 cgggcaagcc gccatacacg ccgggccgtc ttcacgatgg ttgcacaggt cgcatttatt
    11941 ggcttcggct ttgtcagccc gtacattcag acccgcgccg ctgttgcgaa tcacaggacg
    12001 taccaccact tccatcgcgc catacgggca agccaccacg caggttttgc aaccaatgca
    12061 acgttcctgc atcacaggaa caaacccttt atcacggctg atagcaccat tcgggcagac
    12121 gttagcgcac ggtgcatctt cacactgacg gcaaactgtc gccgtggaaa tgttcacacc
    12181 tttaatgaca tggatacgcg gtaaaaatgt ttccggggtc agcgatgcac agtcctgatt
    12241 ttcctgatga gaaaccacgc acgccacttc acaggtacgg caaccaatac atttactcgc
    12301 gtcagcaatg atgaaacggt tcatcaaatt ctccagcaat gacagttaat gcgccgatac
    12361 actcacaaat catgccagtt tttaatttgc tgttatttaa ggaaattaat ttctgtaatg
    12421 caggaaaaaa gatgtcatcg acactagtga cgatgacatg tgatgacaat gtttatcgcg
    12481 aaagggcaat gagtgagtcg cggcggatca gttttccgct aaaggttttc ggcggtgaga
    12541 aatccccgcc atcgagcata aaaatcagcc gtccaataat ttcctgaatc atctcagtca
    12601 ccggaatttt tacgctggag agcgccggaa cgatataggg ggcaattgcg atatcatcga
    12661 atccgataac tgacacctgc tctggcaccg ctacgccgcg ctcgtgcaac gctttgatcg
    12721 cacctatcgc catatcgtcg ttactggcaa ctaacgcgct aaatttagcc cctcgttcga
    12781 gcaacgtttc taccccttcg gccccgctgg cgggcgtcca tttaccgtta gcgataagtt
    12841 tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt atacccggca agacgttcaa
    12901 tgctggtggg ggaatccatc gagccggtaa ggaaagcaat ctcctgatgc ccggcgttta
    12961 tcaactctgc cacggcgtta aagctggtct gtttatgatc gcaccagacg ctatggctgc
    13021 tattattgcg caggcggcga ttaagcacca ttatcggctg actgtgcgcg tcaatgatgt
    13081 catcaatctc atccacgctt aaaaaacgcg ggtaaatcat aatggcgtcg cagcgcaaat
    13141 ccagcagata ctgaatcgcc tggcgctctt cttcggcgct gtgtttacca tctgccaata
    13201 gcaactgtcg ccctttctct tccgccattc gcgcggcatg aaacagtaat tcactaaaat
    13261 aaatgccgtg ataaagcgtg ttggtcacta ccagccccag cgtctgagta ctcttcgccg
    13321 acagattgcg ggccagcaaa tttgggcggt aactgctctc ttctaccgcc tgaaacacgc
    13381 gatctttagt ctcctggctg acgtagccat tacctgaaag cacgcgggaa acggtcgctt
    13441 ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt catcattttc atccctttac
    13501 acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc cttgcgcaat cggctccagc
    13561 ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt aaacatggcg
    13621 attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag ttctttgtac
    13681 ggcaccactt tgctcttctt cttacgcaca tcgactacgg tcatgcggtc ggtacaggag
    13741 tagcaagggt ccaggctacc gataatcagc ggcgcatagg aaacggtatt gccacgcagc
    13801 atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca gcggtacagc
    13861 ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc ttcggcaaag
    13921 cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg accgcccggc
    13981 aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt gattcgcact
    14041 ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg cagcaggcca
    14101 taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc gcgaaccatc
    14161 gggccaacgt tactgaaatc gcgggcgatt tccgggtcca gacgaccaat gccgacggtg
    14221 cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac ttcacgacgc
    14281 atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc gcgacgaatc
    14341 ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc tgccattttc
    14401 atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc ggtaaagtga
    14461 caggcgaggc cgaggttgag cagatgcggg tgcaggcgtt ccacctccag cagaatggcg
    14521 cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac cgacgtggtg
    14581 taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag gaaggtcact
    14641 tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac gtagaacaga
    14701 cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg acccggttcg
    14761 tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc gcccagttcg
    14821 ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg ataatccatg
    14881 ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagcac cagacgacgt
    14941 tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcgcg ctcgccccac
    15001 accgccgccg gaacgcgcgg cgtcacggac ggatactccg gtttgttggc gtcaacttcg
    15061 acgcgaaccg ttacccaaca cttggtgccc ttctccatcg acagcacgta ataaacggcg
    15121 taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca gccaccctgc
    15181 ttgtagtaga gaaactccac cacttccggc agatagttca cctttacggt gacagtcagc
    15241 tgatctttgg tctgccaggc gtggtccagc acgacgccta gaaatgcctc attcagcgcg
    15301 gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt aatcacgccg
    15361 ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc agcccaggta atgcgcggag
    15421 taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc aggacgccga
    15481 ccaccagttt gacgatggca atcaccagcg ccagcagcag tccaccgacg gtgaaggttt
    15541 ccatttgtcc ccacggaata aacaccccga cgaacatctg caacaccacc agctgtttca
    15601 ggctgatacc ccatttcatg acgccaaagc cgctaccgct gtattcggag agcggacctt
    15661 cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt tcgataaaag
    15721 tggcgaacgc acaggcgcag agcgccagca ccagcgggat gctttggctc agcggccagt
    15781 gataaacggt gtcggtaatg ttgctgatgt tggtggaacc ggcaacctgt gcggcaaccc
    15841 acagaccaag cagcaacatc ggttcgacca gcacgccaag catcgcttca cggctcgcac
    15901 cgatagcggt aaacgggcta ccggtatcca gaccggaaat ggcaaagaag aaacgcgcga
    15961 tggcgaagag atacagtaag gtgatcaaat cgcccagttg cggcagcgga gaaccgacgg
    16021 tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc caccatcaca tacggcgtca
    16081 ggcggaacat ccagccagag gcatccgggc caacgctctg gcggcccagc agtttgataa
    16141 tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt atgcagacgg gcgcgcgcca
    16201 cgcgggtaat accggagagc agcggcgcaa cggcaaataa caccagcgcc tgaattaacg
    16261 gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca gtaccgccag
    16321 ttcaaccagc gccatccggc ggaacagcaa cgcactacct tcgcactgcc agcccggcac
    16381 tagcgacacc ggattcagcc atttgcgcag tttcagcact ggcgcaaacg cctgtttaac
    16441 cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatggtcgt agccgcacac
    16501 ccaggccgca ccgcgggaac gtgacggcag gcggtcgcct ttgcaaatcg ccataatgat
    16561 gaatggcagc agcgggcagg caatcagcag caatgtgatc attggttgag aaacggtggt
    16621 gttagcaggc tccagaggca gaggtacagc agcagagagc atcggcagca gccacggcgc
    16681 agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc tcatcaggag
    16741 cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga acgtgacgcc
    16801 atagactttc gccatacaca tcaccgccag cgcaccggta attgccagac ccacagcgag
    16861 cagcggcccc agcagacggg caacaaacgc gccgctattg ctcagtttga agaaggattg
    16921 atagataacc cattccccgg caaacccgtt cagtggcggc agcgcagcca ttgccatcaa
    16981 cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac cgagtttttc
    17041 gatatcgcga tgaccggtac ggaaccagac gctccccgcc ccgaggaaca gtacgctttt
    17101 gaacaggcta tggttaagca gatggtacag accaccgacc aggccaagag caatcagcgc
    17161 cggttgttcg agcgcgatac ccgtta
//
LOCUS       DAZPDE010000082        17090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPDE010000082 DAZPDE010000000
VERSION     DAZPDE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.082"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..509
                     /locus_tag="ACHU0J_002382"
     CDS             <1..509
                     /locus_tag="ACHU0J_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1217047.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(510..1670)
                     /gene="ybdL"
                     /locus_tag="ACHU0J_002383"
     CDS             complement(510..1670)
                     /gene="ybdL"
                     /locus_tag="ACHU0J_002383"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1217048.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1779..2867
                     /gene="hcxA"
                     /locus_tag="ACHU0J_002384"
     CDS             1779..2867
                     /gene="hcxA"
                     /locus_tag="ACHU0J_002384"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1217049.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2877..3074)
                     /locus_tag="ACHU0J_002385"
     CDS             complement(2877..3074)
                     /locus_tag="ACHU0J_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1217050.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3256..5361)
                     /gene="cstA"
                     /locus_tag="ACHU0J_002386"
     CDS             complement(3256..5361)
                     /gene="cstA"
                     /locus_tag="ACHU0J_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1217051.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5542..5955)
                     /gene="entH"
                     /locus_tag="ACHU0J_002387"
     CDS             complement(5542..5955)
                     /gene="entH"
                     /locus_tag="ACHU0J_002387"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1217052.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5958..6704)
                     /gene="entA"
                     /locus_tag="ACHU0J_002388"
     CDS             complement(5958..6704)
                     /gene="entA"
                     /locus_tag="ACHU0J_002388"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1217053.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6704..7561)
                     /gene="entB"
                     /locus_tag="ACHU0J_002389"
     CDS             complement(6704..7561)
                     /gene="entB"
                     /locus_tag="ACHU0J_002389"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1217054.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7575..9185)
                     /gene="entE"
                     /locus_tag="ACHU0J_002390"
     CDS             complement(7575..9185)
                     /gene="entE"
                     /locus_tag="ACHU0J_002390"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1217055.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9195..10370)
                     /gene="entC"
                     /locus_tag="ACHU0J_002391"
                     /pseudo
     CDS             complement(9195..10370)
                     /gene="entC"
                     /locus_tag="ACHU0J_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10662..11618
                     /gene="fepB"
                     /locus_tag="ACHU0J_002392"
     CDS             10662..11618
                     /gene="fepB"
                     /locus_tag="ACHU0J_002392"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1217056.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11622..12854)
                     /gene="entS"
                     /locus_tag="ACHU0J_002393"
     CDS             complement(11622..12854)
                     /gene="entS"
                     /locus_tag="ACHU0J_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1217057.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12965..13969
                     /gene="fepD"
                     /locus_tag="ACHU0J_002394"
     CDS             12965..13969
                     /gene="fepD"
                     /locus_tag="ACHU0J_002394"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1217058.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13966..14958
                     /gene="fepG"
                     /locus_tag="ACHU0J_002395"
     CDS             13966..14958
                     /gene="fepG"
                     /locus_tag="ACHU0J_002395"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1217059.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14955..15770
                     /gene="fepC"
                     /locus_tag="ACHU0J_002396"
     CDS             14955..15770
                     /gene="fepC"
                     /locus_tag="ACHU0J_002396"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1217060.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15767..16900)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU0J_002397"
                     /pseudo
     CDS             complement(15767..16900)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU0J_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         3994 a         4863 c         4443 g         3790 t
ORIGIN      
        1 cctttcgtga aaagccagtg gattgcgttc tgtgggttaa aaacagccaa cttatgccta
       61 atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa actgctgcaa aaatccattg
      121 aaatagatgg ttttacccaa cccattgtgg tcacacatac ggataaaaat gccatggaaa
      181 tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta aaattacgct
      241 taaaaggtta tttgccggtg acctgtctgg aaggtacgcg taatcaacgc atcgccgcga
      301 ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg gaaatcgtcc
      361 gtgaacttag ccagttggga tgggatgata ataaaattgg caaagagctg ggaatggaca
      421 gcgacgaagt gctgcgactg aagcaaatta acggcctgca agagttgttt gccgaccgtc
      481 aatattcccg cgcctggacg gttaaatagc taaagctggc gcaggcgttc agctgctgcc
      541 agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgtgg gaagggatcg
      601 gcgcaaaaca ccgacagcgg aatcgccgct acgccgtgct cctgcgtcag ccactggcaa
      661 aactcaacat catccagggt agaaaccgcg ctgtaatcca ccagcaaaaa gtatgtacct
      721 tcacacggta aaatctccag ccggctttca tttaaagcat tcaccagaat atcgcgcttc
      781 tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag catatcagca
      841 agcgccagct gtgccggggt attcaccgaa aaggtcagat actgatgtac cttgcgaatt
      901 tcggcgctga tgggcgctgg cgcaacacaa taacccactt tccagccggt catatgatag
      961 gtcttgccaa atgaagaaac cgccactgcc cgctcacgca gctgcggatg cgccagcaca
     1021 ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct aatgacaaaa
     1081 atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg ccagacagtt
     1141 gcactggggt tatgcggagt gttgaggatc accagtctgg tgcgctcgct taataatgcg
     1201 gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg tttcactatt
     1261 cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa acaaatcact
     1321 tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt cgcccctgcc
     1381 gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc cgttttctga
     1441 gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt tgccccctgg
     1501 gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc aggaaagcct
     1561 tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt gaaaatagtg
     1621 gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat ttttattgta
     1681 cctgacgaag ttgcgtgttg aaggcactat aacacgatgc cagtatttgg caatcaagac
     1741 gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat cctatccgcg
     1801 tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac ctgcacaatt
     1861 ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc gccattgctg
     1921 cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat attttgtttc
     1981 gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt gacgatcgca
     2041 gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc ctcgcccgcc
     2101 gtctcggtct gccatttgtt gccgctccga cgatcgccgc cacctgcgca gcctggacac
     2161 cgctctccgt ctggtataac gatgccggac aggcgctgca ttatgagatt ttcgacgacg
     2221 ccaattttat ggtgctggtg gaaccggaga ttatcctcaa tgcaccgcaa caatatctgc
     2281 tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac
     2341 cagaaacgtt gccgctaacc gtgcggctgg ggatcaataa tgcgcaggcc attcgcgacg
     2401 tcttgttaaa cagtagcgaa caggcacttg ccgatcagca aaatcagcag ttaacgcaat
     2461 cattttgcga tgtggtggat gctattattg ctggtggtgg gatggtcggt ggtctgggcg
     2521 atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa cggtctgact gtcctgccgc
     2581 aaaccgagaa gtttcttcac ggcaccaaag tcgcttacgg aattctggtg caaagcgcct
     2641 tgctgggtca ggatgatgtg ctggcgcaat taaccggggc gtatcagcgt tttcatctgc
     2701 cgactacgct ggcggagctg gaggtggata tcaataatca ggcggagatc gacaaagtga
     2761 ttgcccacac cttgcgcccg gtggagtcca ttcattacct gccagtcacg ctgacaccag
     2821 atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt gcaaatttag
     2881 cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg gaaaaactcc
     2941 tcgtaggtca tcggcgtttg atcgggatga ttaacccgca tatgttcgac atagttgtcg
     3001 taatcaggca taccaatcat cagcttcgcc gcctgaccta aatattttcc ggcttttgcc
     3061 agtgaatcaa acatcttggc ctccgctaac catgttgctg aaagagtgcc ggatgcggcg
     3121 tgaacgcttt atccggccta caaatgctat caacgcactc ggtcagtccc ctcgccccat
     3181 cggggagagg gttagggtga gggggaaccc tcggcactat gccaacattc gccaacatcc
     3241 cccctcactc tgactttagt gcgcgccttt cgcctgcgct acgatctcct cgacattttc
     3301 cggcatcggc tcatacggcg tttctttcgc cgttggcttc ggatctttca atgccgcaag
     3361 tgccgtctta atcgagaaca gtgccaggac caccacgacc accataaaga agatggttaa
     3421 cccggcatcc agacggttgt tgaacaccag ttgcgccagc tgtgactcag tatactgcga
     3481 cggaatattg ccgctgtcga tcattgcctg gaacttgtta gcaatgacca ggaagccgac
     3541 tttcgcatcc gggctaaacg ctttcagcca gtcagcggtc agggtacaaa tcagcagcca
     3601 ggccgttggt accagcgcca cccaggcgta acgttgacgt ttcatcttga acaacaccac
     3661 ggcacagagc atcagcgcca tccctgccag catctggttg gcaataccaa acagcggcca
     3721 cagagtgtta ataccgccca gcggatcgac cacgctctga tggaggaagt agccccacgc
     3781 cagtacgcac agcgctgttg ccagcaggtt agcaggcagt gaatcggtcc gcttcaggcc
     3841 aggagacacc acgcccagca gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc
     3901 taccgccgtc agaataaaca gagcttcaaa cagaatggcg aagtgatacc agaacgccac
     3961 atccatcata ccacccagcg caccgtgcag gatgtaagcc atccccaccg ccaacgtcgg
     4021 cgcaccgccc gcacgggaaa tgatcgactg ttcacccact tcgctggcaa tctggttgag
     4081 cgtatctggt gtaatgctaa agccccagct actcaccacc tgcgcggcag aagcgactac
     4141 atccgccgtc cctgccggag ccagcaccgc cattgggctg ttcatggcaa aatacacgcc
     4201 cggatcgatg atacaggcag aaactagcgc cataatcgcc acgaaggatt ccatcaacat
     4261 cccaccgtag ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg gcgtggtgcc
     4321 ggaagagatc agcgcatgga agccagacac cgcaccacag gcgatggtga taaacaggaa
     4381 cgggaacagg ttaccggtcc ataccgggcc agtgccatcg acaaatttgg tcagcgcagg
     4441 catggtcagc gtcgggcgca taatcaaaat gcctaccgcc agaccgacga tagtcccgat
     4501 tttcaggaag gtcgagagat agtcacgcgg ggccagcagc aaccacaccg gcagcaccgc
     4561 cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc acgccggtaa agtcaaagta
     4621 cggtgcccag gtcggacttt ctgccaccca gccgccagag ataatggcga aaatcaggaa
     4681 caccaggcca atcactgaca cttcgccaat gcggcccgga cgcagatagc gcaggtagat
     4741 tcccataaaa atcgccagcg gaatggtgaa cgcaacggta tacgttcccc acgggctatg
     4801 agtcagggct ttcaccacga tcatcgccag tactgcaagg ataatgacca tgatcataaa
     4861 gcaggctacc agcgcaatca ccccggcagt tggccccatc tcttctttga ccagctcacc
     4921 cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc
     4981 ggcgagaacc accccggcga gcagccagat catccccggc aggtagccca tttgcgctgc
     5041 cagcaccggc cccaccagcg gccctgctcc ggcaatggcc gcaaaatggt gaccgaacag
     5101 cactttcttg tccgtcggca catagtccag cccgtcgtta tggcgcaccg ctggcgtcat
     5161 acgcgtcggg tcaaccgcca gcacattttt ggcgatatac agcccataaa aacggtaagc
     5221 gatcagatag atacagaccg acgccaccac aatccacagc gcgttgatct gttccccacg
     5281 atttaaagca atgtatccca gagcaaatgc tcccattaca gagagcactg tccagacgag
     5341 gtatttccct gatttgttca tagttgttat ccgtgtgcgt gtccatagag atgttacatt
     5401 ttgtttctat aacatctttg tacgatttaa caacacggta accatgtaaa tcggagttga
     5461 aaccaatatt taacttaaca atgttaactc gatcactccg ttaaccgagt tttcctgcaa
     5521 tctcatttaa ttctgtcggg ctcatcccaa aactgccgta cccagccgac aagtgcagca
     5581 acgccgcccc tgttcatcga aaacgacgat ttcccaactc tgattttgcc tcccaagatg
     5641 cagcggctgg cagacgccgc gtaccttgcc ttcagacacc gggcgatgat gggtggcgtt
     5701 aagttccgtg cccaccacac actgcccgtc acgggtcatc ataaatccgg ccatcgatcc
     5761 cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc aggccaaacg gctgatgagt
     5821 acgggtatca accggcattt cggcttccag cacatcatct cccagaaggg tatacacaat
     5881 tcccagatgc gccaccattg tgttatcgct ggtggcgttc agttcgtcga gcgttaaatg
     5941 gcgtttccag atcatgctta tgcccccagc gttgagccgc catcgaccac aatatcctgt
     6001 agggtgatat ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt
     6061 ggacgggcga ttttccccag cggaatgccg agtttaaact gctcgccaaa gccgcgaata
     6121 cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt gtcggtggag
     6181 ccaggcgaaa ccacattaca gcgcacgccg ctacccgcca gttccagccc gacgctcaac
     6241 gccaggcttt tcagcgccgc tttcgatgcg ccataagcac tcataccaat acgcgccgtg
     6301 tgtgcggcgt cggacgccac agtgacaatc gccccgcccc gctgacggcg aaactggttc
     6361 atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt ctgctgccag
     6421 tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc attgatcagc
     6481 acgtccagtc gctccgtttc agctaacagt cgctgacata cctgcgccac ctgcccggcg
     6541 tcggcaacat ccattacttc ggtcgcaaag ggatattgct cctgagtgaa cgcttgatca
     6601 aaacctgtaa ctttcgctcc cgcctcaaca aacgtcagcg ccgtggcgta gccgatacct
     6661 ttacctgcgc cggttaccca gacattttta ccgctgaaat ccattatttc acctcgcggg
     6721 agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat
     6781 caccatgcac tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa tccagaccgt
     6841 agtcgatcag gttgtcgtca tcgaacggtt catcggactc gtccagcaac ggcaggatca
     6901 cctcacgcag cgccgctttg ctggcgggga taggtgctgg cagtaattct tcggtcatca
     6961 ccacccggcc agaacgtccg gccacatatt tcagcgacat caaatgctcg tcacggctga
     7021 aatcagccag cgcatccgcc accataaacg gtttaatatc gcgcataaat gcgtcggttg
     7081 cggtggtcat acagccaatg tgggcatata ctcctgtaat aatcagctgg ttacgtccac
     7141 tctctttgag catttgctcc agcggagaac gatgaaacgc gctgtagcgc cacttcacca
     7201 gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac cttttgctgt tccggcgagc
     7261 gggtcaggcc cggcccccac atatcattca acagcgcccg atcttcatcg ctctgctctt
     7321 tcggctgggc ggtgtaataa accgggatat tgtgctgttt gcagtagtcg cgcagcgcag
     7381 caatattcgc gatcacctgc tccatcatcg ggcagttctc gccccagaag ctgacaaaat
     7441 agtcctgcat atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc cagtcaactt
     7501 tattctgcgg aatatcgtga gactccggca gtgcgtaagc ctgtaatttt ggaatagcca
     7561 tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta
     7621 tcgactttcc cgaccgccgt cagcggaagt gaatccacac actccacgcg atccggtaat
     7681 ttaaattcgg caataccctg ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc
     7741 tcttttacca ccagataagc acagcttttt tcgcccatca gctcattttc catgctcacc
     7801 agtgcggcat agatcactgc cggatggctc agcagcaggt tttcgatatc ttcggcagcg
     7861 atcttctcgc cgccacggtt gatctgatct ttctcgcgcc cctgcacggt gatgtaaccc
     7921 tctggatcaa tagagatcag atcgccggaa cagtaaaaac cgttggcatc aaaggcactg
     7981 gcattgtgct gcggactttt gtaatagcca cggaaggtgt acggcccgcg cgtcatcagg
     8041 cgtccaactt ccccttgcgg cagtggattt ccttcggcat cggcaaccca cacttcatcg
     8101 tccggacaca ttgggtaacc ctgggtatgg ataattttct ccgcgctatc atcaagacgg
     8161 gtgtagttca ccagcccttc cgccatgcca aacacctgct gcaactggca gccaatctca
     8221 gcgggaatac gcgctgcaag cgtggcagaa agacgtgcgc cgccgacctg taacagtttc
     8281 agcgaggcaa gctgcgcccg gctttcgcct tcagccagcg cctgcaacca caggctgact
     8341 gccggcggca ccagcgcggt aacgttaacc tgatgttttg caatcaacgg gaagcaaagc
     8401 gtggcgctgg gatcggcagc cagaacaacc gttcctccgg caagaaagac gcccagcgat
     8461 cctggcgaac tcatagtgta gttatgagcc gccgggatcg agcacaggta gcgcgtctgt
     8521 tgtgtgaact gacaaatctc ggcgctacga cgcacgctgt agtaatagtc gttatgagtg
     8581 cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa gctggaaata ggccacttca
     8641 tcagcaggtg atggcgtggc agtaaaaccc tctgccggat ggttaatcgc atcctgcaag
     8701 ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa tggaagaatg ctctgcaaca
     8761 aatgtgttga ggaaatcatc cccgctaaat agcgcatgct ggcgatcggc aatcagtaac
     8821 gccggttcaa tctggctggc gtaggcgttc agttcactac gctgatggct gaacagcgcc
     8881 agcaccggcg cgacgcccag tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg
     8941 ttacccagtt gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag
     9001 gcgaggttat ccgccgcctg attcagctcc cgataactca actgccgctc gccgtcgata
     9061 accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc
     9121 cagtagcctt tttcccgata gcgacgggca aactcttccg gccagcgggt gaatggaatg
     9181 ctcatcctcg ctccttaatg caatccaaaa acgttcaaca tggtagaaag tttgacgcct
     9241 gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa tccccgctcc ggcaaacaga
     9301 cgcacctgat tttcccgcag cttcgcgcag cggatggtca ccacccattc gccgttacct
     9361 tcgctgtcac accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca
     9421 gcaataacct gggtcgcggc ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc
     9481 agacaggcca gagtcagtgc gttttcttgc gaattcgctt taccttcaaa gggagttgcg
     9541 agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg taactcacta
     9601 ctgcgttcgc gcagtacctc tttcatcgcc tgagtcccca gttcatgttc atggcgatct
     9661 ttttctgacg ccagcagacg attacccgct tcgcgatcga gcacttcatc cggctgacga
     9721 cgcgcggaac cggctaacgg aatggagcta aaacgctcgc cgtctttacg tagcagcagt
     9781 tccgggctgg cccccagcag gacgccacca tcagccagcg ggacatggaa gttgtaacta
     9841 accgggtttt gcgcaatcaa ccgttccagc aatacgccac tatcaatggc ggcgtcagtg
     9901 gtgatatcaa tcaatcgtga caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg
     9961 gcggcgcggg cacccatctg ttcaaacgtg gtttgttcag gaattgccta gcgttccacc
    10021 acgttcagcg actggctgcg ggtaaaacag cgggctgagg tctgtttttc ctgacgggag
    10081 aacgactgct aggattcggg aatatacagc gacgaaggct gacgtggatc gaagggaatc
    10141 gccccgacca tcaccggatt tttgatgccc tgcgctttgg catcggcaaa cagcgcggcg
    10201 agtttttgct ggaagggact gtcgggcgaa tccccgttca cagccggttc atcgaaacgg
    10261 gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc
    10321 gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg acgtatccat atcatcctcc
    10381 acaaaatgat aaaggcttta ataatgatta tcacttatat tttcggccgc taacctaaaa
    10441 gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct gcaactcggg gttgcatctg
    10501 gttgtggtta caatgaaaat gagaagcatt attgatgaat tcgcataagc gcaatgtgat
    10561 gtcctgcgcc gttttgcccc ctctccccaa agggacggat tgtgcgcttt gtcgaatttg
    10621 tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc gccccgctct
    10681 accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta gttcaggccg
    10741 ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa agccagccgc
    10801 agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt gatgctccgg
    10861 tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag ggctttttac
    10921 gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga
    10981 gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg
    11041 attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt
    11101 acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gggcatgaga
    11161 aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa
    11221 tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca
    11281 atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg
    11341 cgaaattgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc
    11401 agcttggtgg ggaaaatctg gctgcggggt taaacggtga atcgctattc ctgttcgctg
    11461 gtgatcagaa agatgccgat gcgatttatg ccaatccact gctcgcgcac ctgcctgcgg
    11521 tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt ccgcctggat tactacagcg
    11581 ccatgcaagt gctggaacgg cttaaggcgc tgttttaagc attaactgtc ggacgctgtc
    11641 acctgcggcg gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg
    11701 ccgataatca acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgcg
    11761 cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg tcacgttctg cgccgtccac
    11821 aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtatattgc
    11881 agcaacgaac taaccgcgct cagccagccg aacagcgcca gacaaaccac gcctaaaatc
    11941 cacatcggca tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc
    12001 atcaataacc ctggtcgcgc actatgtgcc agcttcccgc tggttaacgc accaatagcc
    12061 gcgccgagcg ggatcgccgc gtagagaaaa ccaatctgtg ccgctgacat ctgccagttg
    12121 tcagccagcg ccggatacag taccagcacc gcgctcgcca tcgtcaataa accacccagc
    12181 agcgggctgg caagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcaagc
    12241 ggctgcggtg gcggtggcaa ctccggaagg cttaataacg gtaacaaggt aataaacgtg
    12301 cccgccgccg ccagcccgta gttccaggct acgccgccgg tcgccagcaa taaaccgcca
    12361 atcatgggcg aaatcaccga ccccaggcgc acggtcaaca tggtgatcgc cccggcctgc
    12421 attaagtttt cacgccctac cagtgcagat gttgccgcca gtagcgccgt aacaccaagt
    12481 gatgcgaaaa aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc
    12541 agcagtgcat taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa
    12601 atcacttttt tgcgttcata gcgatccgcc agcacaccgc caaccatcag gccaacaaac
    12661 atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc aggtagaatg ggtcatcatc
    12721 tggatctgca ccggcaccgc gacgccgagc aaacccagag acacaattga gataaaacga
    12781 gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag
    12841 gattgtttat tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg
    12901 cacatgttaa catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt
    12961 tcgtatatct ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt
    13021 actgattatc gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt
    13081 agtgctggag gccttctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc
    13141 gcggctgccg cgtacccttg ccggtttact ggcaggcggc gcgcttggcc ttgccggggc
    13201 gttaatgcaa accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtgaacgc
    13261 cggagccagc tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcacagga
    13321 acaactggcg atggccttcg ccggggcgct ggtggcctca ttgattgttg cctttaccgg
    13381 cagtcagggt ggagggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc
    13441 ggcggtgctg gaaggactga ccagcggcat cgccctgctt aatcctgacg tctacgatca
    13501 actgcgtttc tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt
    13561 gctgatcccg gttctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag
    13621 tttgagcctc ggcagtgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt
    13681 gattggtctg ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat
    13741 tgcctttatc ggcctgatga tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg
    13801 ctggtcgctg cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat
    13861 cgggcgggtg attgtgcccg gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc
    13921 accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct
    13981 cgccgattac tcatcacctg tttgctgctg atttccgcct gtgtggttgc aggtatctgg
    14041 ggattacgca gcggtgccgt cacgctggaa atctcgcagg tattcgccgc gttgatgggc
    14101 gatgcgccgc gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg
    14161 gcgctgttga ttggcgcggc gctgggcgtc agcggcgcga tttttcagtc gctgatgcgt
    14221 aacccgctcg gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg
    14281 gtggcgatgg tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggtgggc
    14341 ggcattatca cttcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt
    14401 cggttgatta ttatcggtat cggcgttcgc gccatgctgg tggcctttaa tacctggctg
    14461 ttgctgaaag cgtctttaga aacggcgcta acagcaggtt tgtggaatgc cggatcgctc
    14521 aacggcctga cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctcatt
    14581 gccgccacct tgctggtgcg gcgaatgcgc ttgctggaga tgggcgatga taccgcctgc
    14641 gcgctgggcg tcagcgtcga gcgttcgcgt ctgttaatga tgctggttgc agtggtgctt
    14701 accgctgcgg caacggcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac
    14761 attgcccgac gcattagcgg caccgctcgc tgggggctga cccaggcggc gctgtgcgga
    14821 gcgctgttac tgctggcggc cgttctctgc gcccaacaac tgtttatgcc gtatcaactt
    14881 ccggttggcg tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag
    14941 gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg
    15001 gatatggcaa atataccgtt gcggaaaatc tgactgtaga aattcctgat ggtcacttca
    15061 cggcaattat cggaccaaat ggctgtggta aatccacgtt actgcgtacc ttaagccgcc
    15121 tgatgacgcc tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca
    15181 gtaaagaagt tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata
    15241 tcaccgtgca ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct
    15301 ggcgcaaaga ggatgaagaa gcggtaacga aagcgatgca ggccacggga ataactcatc
    15361 tggcagatca aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatcgcga
    15421 tggtgctggc ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata
    15481 tcagtcatca gattgatcta ctggaactgt taagcgaact gaaccgcgag aaaggctata
    15541 ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg
    15601 cattgcggga agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac
    15661 tgattgagcg catttatggt ctgcgatgca tgatcattga cgatccggta gccggaatgc
    15721 cgcttgtggt gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt
    15781 ctgccatcat ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac
    15841 aagccaccat cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt
    15901 cttttttcac tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt
    15961 tgacatttgc ttttgttaat tgctcgacaa gatactgccg attacgtaat tcaccgttca
    16021 gttccgcaac gtcagtaacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc
    16081 cgagggaaat agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct
    16141 ttttaattcc tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat
    16201 caagttgatt tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt
    16261 tgatctccag tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat
    16321 aatcgatata cccgctcaaa acggtctgcg cctctttact ggttggcgcg gtaaaactta
    16381 gcgtccagga tgtatacagt gacggttcat cttttttctt actagcattg tcatcaaccg
    16441 ctttcatttt ttcgctcaat gcaacaatag cgcgatgcaa atccagttca tcgattttcg
    16501 cctcttttaa ttagtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa
    16561 cagactgaaa cttcttgata aacaggttaa atgcaccgcc acggtcgata ttaacatcga
    16621 gatcaagaac gcggagtttg gtaaaggttt tctccaggtc ctgccactga atggcttcgg
    16681 cgggagtaat aacggctgag ctggtccatt tttgcggcag gatgaaagag atcagtaaac
    16741 ctgcgcaggc aaatgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt
    16801 cgattagatt aagtaaatca atttcattat tactgggcga cgccagagga tactcagaaa
    16861 aatgagcgtc acttccctgt ttaatattca atgatgacat ggggtataac ctgaaaatga
    16921 gtccaatagg ggaaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag
    16981 ctgaagtttg taaattcctt ccactgaatg caacattcac tctatttaag agaaatattc
    17041 atcaaaccta taaatcaaac aaataaaaaa cccataatta ttttctgtaa
//
LOCUS       DAZPDE010000083        17088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPDE010000083 DAZPDE010000000
VERSION     DAZPDE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.083"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            82..1221
                     /gene="arnB"
                     /locus_tag="ACHU0J_002398"
     CDS             82..1221
                     /gene="arnB"
                     /locus_tag="ACHU0J_002398"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1217061.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1225..2193
                     /gene="arnC"
                     /locus_tag="ACHU0J_002399"
     CDS             1225..2193
                     /gene="arnC"
                     /locus_tag="ACHU0J_002399"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1217062.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2193..4174
                     /gene="arnA"
                     /locus_tag="ACHU0J_002400"
                     /pseudo
     CDS             2193..4174
                     /gene="arnA"
                     /locus_tag="ACHU0J_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            4171..5061
                     /gene="arnD"
                     /locus_tag="ACHU0J_002401"
     CDS             4171..5061
                     /gene="arnD"
                     /locus_tag="ACHU0J_002401"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1217063.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5061..6713
                     /gene="arnT"
                     /locus_tag="ACHU0J_002402"
     CDS             5061..6713
                     /gene="arnT"
                     /locus_tag="ACHU0J_002402"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1217064.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            6710..7045
                     /gene="arnE"
                     /locus_tag="ACHU0J_002403"
     CDS             6710..7045
                     /gene="arnE"
                     /locus_tag="ACHU0J_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1217065.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7045..7431
                     /gene="arnF"
                     /locus_tag="ACHU0J_002404"
     CDS             7045..7431
                     /gene="arnF"
                     /locus_tag="ACHU0J_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1217066.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7425..7697)
                     /gene="pmrD"
                     /locus_tag="ACHU0J_002405"
     CDS             complement(7425..7697)
                     /gene="pmrD"
                     /locus_tag="ACHU0J_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1217067.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7802..9157)
                     /gene="menE"
                     /locus_tag="ACHU0J_002406"
     CDS             complement(7802..9157)
                     /gene="menE"
                     /locus_tag="ACHU0J_002406"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1217068.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9154..10116)
                     /gene="menC"
                     /locus_tag="ACHU0J_002407"
     CDS             complement(9154..10116)
                     /gene="menC"
                     /locus_tag="ACHU0J_002407"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1217069.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(10116..10973)
                     /gene="menB"
                     /locus_tag="ACHU0J_002408"
     CDS             complement(10116..10973)
                     /gene="menB"
                     /locus_tag="ACHU0J_002408"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1217070.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10988..11746)
                     /gene="menH"
                     /locus_tag="ACHU0J_002409"
     CDS             complement(10988..11746)
                     /gene="menH"
                     /locus_tag="ACHU0J_002409"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1217071.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11743..13413)
                     /gene="menD"
                     /locus_tag="ACHU0J_002410"
     CDS             complement(11743..13413)
                     /gene="menD"
                     /locus_tag="ACHU0J_002410"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1217072.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13502..14797)
                     /gene="menF"
                     /locus_tag="ACHU0J_002411"
     CDS             complement(13502..14797)
                     /gene="menF"
                     /locus_tag="ACHU0J_002411"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1217073.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14876..15181)
                     /gene="elaB"
                     /locus_tag="ACHU0J_002412"
     CDS             complement(14876..15181)
                     /gene="elaB"
                     /locus_tag="ACHU0J_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1217074.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15236..15697)
                     /locus_tag="ACHU0J_002413"
     CDS             complement(15236..15697)
                     /locus_tag="ACHU0J_002413"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1217075.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15762..16679
                     /gene="rbn"
                     /locus_tag="ACHU0J_002414"
     CDS             15762..16679
                     /gene="rbn"
                     /locus_tag="ACHU0J_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1217076.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16870..17058
                     /locus_tag="ACHU0J_002415"
                     /pseudo
     CDS             16870..17058
                     /locus_tag="ACHU0J_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         3981 a         4627 c         4344 g         4136 t
ORIGIN      
        1 tcgctccaaa tggcaaaata tacacaacac tctttatagc aaatataagt ggacaggtat
       61 tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt tttcgcgacc agcaatgggc
      121 gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg gttggatcac aaccggtccg
      181 aagaatcagg cgcttgagca agccttttgc cagttgacgg gaaatcagca tgccatcgcg
      241 gtcagttcag ccaccgccgg aatgcatatc acgctaatgg cgttggaaat tggcaagggc
      301 gatgaagtga ttacgccttc cctgacctgg gtttcaaccc tcaatatgat ttccttgctg
      361 ggtgcaacgc cggtaatggt ggatgtcgac cgcgatacgc tgatggtcac gcctgaagcg
      421 atcgaatcag ccattacgcc acgcactaaa gccatcattc cggtgcatta tgccggtgcg
      481 ccagcagata ttgacgccat tcgcgccatt ggcgaacgtt acggcatcgc agttatcgaa
      541 gatgctgccc atgccgtcgg tacgtattac aaagggcgac atattggcgc aaaaggtacc
      601 gctatttttt catttcatgc cattaaaaat attacctgtg ctgaaggtgg cctgattgta
      661 actgataatg aaaaccttgc ccgccagcta cggatgctga aatttcacgg tctgggtgtc
      721 gatgcctatg acagacaaac ctggggccgt gcaccgcagg ctgaagtctt aacaccgggc
      781 tataagtaca atctgaccga tattaacgcc gcgattgccc tgacacagtt agtcaaatta
      841 gagcacctca acacccgtcg gcgcgaaatt gcccagcaat atcagcaagc actggcagct
      901 ctcccctttc agccattaag ccttcccgcc tggccgcacg ttcacgcctg gcatctgttt
      961 attattcgtg tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat ggaagcgtta
     1021 aaagaaagag gcattggtac cgggttacat ttccgcgccg ctcacacaca aaaatattat
     1081 cgcgagcgtt ttcccacgct gtcgttaccg aataccgaat ggaatagcga acgcatctgt
     1141 tcgttgccgc tgttcccgga tatgactacc gccgatgccg accgcgtcat cacagccctt
     1201 cagcaactcg caggacaata agccatgttt gaaatccacc ctgttaagaa agtctcggtg
     1261 gttattcccg tttataacga gcaggaaagc ttaccggaat taatcaggcg caccaccaca
     1321 gcctgtgaat cgttggggaa agagtatgag atcctgctga ttgatgacgg cagtagcgat
     1381 aattccgcgc atatgctggt cgaagcctca caagcggaga acagccatat tgtgtctatt
     1441 ttgattaacc gcaattacgg gcaacattca gcgattatgg cgggattcag tcacgttact
     1501 ggcgacttaa ttattaccct tgatgccgat ctccagaatc cgccagaaga aatcccccgc
     1561 ctggtggcaa aagccgatga aggttacgac gtggtaggga ctgtacgcca gaaccgccag
     1621 gacagctggt ttcgtaaaac agcttcgaag atgattaacc ggcttattca gcgcaccact
     1681 ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca tattgtcgat
     1741 gcgatgttgc actgccatga acgcagcacc tttatcccga ttctggcgaa tatcttcgcc
     1801 cgccgtgcaa ttgaaattcc agtacatcat gccgagcgtg agtttggtga atccaaatac
     1861 agttttatgc gcctgattaa tttgatgtac gacctggtga cctgccttac cacaacgccg
     1921 ctacgtatgc tgagtctgct cggcagcatt attgcgattg gaggttttag cattgcggtg
     1981 ctgctggtga ttttacgcct gaccttcgga ccacaatggg cggcagaagg cgtctttatg
     2041 ctatttgccg tgctgtttac ttttattggc gctcagttta tcggcatggg attactcggt
     2101 gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc cccgctattt tgttcagcaa
     2161 gttatccgtc catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt gcctaccacg
     2221 atatgggatg cctcggtatt gaagctctgc tggctgccgg ttacgaaatt agcgccattt
     2281 ttacccatac cgataatccc ggtgaaaaag ccttttatgg ttcggtggct catctggcgg
     2341 cggaaagaga cattccggtt tatgcgccgg ataacgttaa tcatccgctg tgggtggaac
     2401 gcattgccca actgtcacca gaggtgattt tctcttttta ttatcgccat cttatttgcg
     2461 acgagatttt tcagctcgct cccgcaggtg catttaatct gcacggttcg ctgttaccaa
     2521 aatatcgtgg tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaacg gaaacgggcg
     2581 ttacattgca ccgaatggta aacgtgccga tgctggggcc attgtggcgc aactgcgcgt
     2641 tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg
     2701 ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca
     2761 gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt tccttgaatg
     2821 gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg
     2881 tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca
     2941 tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg
     3001 cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc
     3061 gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg
     3121 caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca
     3181 tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag
     3241 cgatgcgata agccgttttc tgaatcatcc acattttcac tttgtcgaag gcgatatcag
     3301 tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct tgccgctggt
     3361 ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt
     3421 tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa tcatcttccc
     3481 gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa
     3541 tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa aacaattact
     3601 tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc tcttcctccc
     3661 gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg gcagctcccg
     3721 cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc taattgatgg
     3781 cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt tgtaccacat
     3841 tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca atcctgagaa
     3901 cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct
     3961 gcgccatcat ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagct gctactacgg
     4021 caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc accgctgcct
     4081 ggactgggag ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct
     4141 gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag taggcttacg cattgatgtc
     4201 gatgcctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat
     4261 aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg
     4321 cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc atcgctttat
     4381 ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat
     4441 gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gtctgcacgc ctgggatcac
     4501 catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga cgatattgca
     4561 cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc tgccgctgcg
     4621 ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac
     4681 agcgattgtc gtggggccat accgttccgt ccactgcttg aatcaggaaa ccctggcact
     4741 gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca
     4801 gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac gccggtttat
     4861 accattcatg cagaagttga atgctgcgct tatcagcata attttgtgga tctcctcaaa
     4921 cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc agagacgttg
     4981 ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa
     5041 caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcatc ttcgcgttta
     5101 ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc
     5161 gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg ccccatctgt
     5221 tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat
     5281 ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa
     5341 ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt ctggctctac
     5401 tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc
     5461 tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc
     5521 aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca
     5581 tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat
     5641 gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca
     5701 gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag cctgacttct
     5761 ggcactattt tttctgggtt gagcatattc aacgctttgc actagatgat gcccaacata
     5821 gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg tggctgggat
     5881 tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca accgtctatt
     5941 tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc aaattgccca
     6001 cctatattct ttcttgcttt gcacctctgg caatgctgtt ggcgcattac gcgttgctgg
     6061 cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc gcttttggcg
     6121 tcactggcat tattgccaca tttgtgatct ccccctgggg accaatgaac acgccggtgt
     6181 ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg ctatgggcat
     6241 ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtctttcgcc gcgctttgcc
     6301 cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg gaaggaaaac
     6361 atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta
     6421 ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca
     6481 tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg aaagggagat
     6541 ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta
     6601 cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt ccgcccgcag
     6661 atgccatcgt tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa tgatctggct
     6721 aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac
     6781 ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct
     6841 ggcttgtctt ggtcttgcca tgatgctctg gctgctggtc ttgcagaacg taccggtagg
     6901 cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg cagtaaaact
     6961 gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat
     7021 tgtgatcctc gggagtacgg tgtaatgtgc ctgatatggg gattattcag cgtcattatt
     7081 gcctcagttg cgcaattaag cctgggtttt gcggcaagtc atctgccgcc gatgacgcac
     7141 ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat actgttactc
     7201 gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca taaacttgcg
     7261 ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg
     7321 gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt
     7381 atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg agttttccct
     7441 gccgctgtac actgccgttc ccactcatcg gggctataac tgcttgccga gaggactttc
     7501 accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg cgagagtaaa
     7561 tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca
     7621 gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga ttttttgtgc
     7681 cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt acagattaac
     7741 agataaactc tttatcaacg gcaatggaat ggcagaaatg agaagtgaaa cggagggtta
     7801 attattgctg acgttgcacc cactctttta gcgcctgacg tgaaatttta ataccgccgt
     7861 ttttcagctc cggcggtaaa gtcagccagc gcaccggttg ttgaaaacga gccagcttat
     7921 ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc gctaccggcc
     7981 gatgaccaaa ctccttgtcg gcaacggaaa cgataaacac ctgcaaaacc gcaggatgtg
     8041 cagcaataac acgctcgact tcttccggct gaataccctc accgccactg aagaacagat
     8101 tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga tcgcgcgtag
     8161 cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag taaccttctg
     8221 ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc cgacccggca
     8281 gcggcaaacc aacgtctgcc aggccgtcgg cttctttcgc acataccgtg gaggcaaact
     8341 cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt tccgtcaact
     8401 cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa ctgcggttaa
     8461 ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct gccagcattt
     8521 gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac cagcgccaca
     8581 taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca tcgtgatcgc
     8641 caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga taagtatgga
     8701 cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac agacgcgtcg
     8761 gctgccacgc agcggcatgt acgccttcaa ccagctgaat gtgcagcgac gttaatgccg
     8821 gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg ggcagcaatt
     8881 cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc ccgcattgca
     8941 gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac atcacgccgc
     9001 tgccctcaac caccccctgc gccgcaaagc ccgtagctaa ctcatcgacg cgagcacaaa
     9061 gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct ccccgcactt
     9121 gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcct gtacatcagc
     9181 gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct gcatcagatc
     9241 cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc gcgccagttg
     9301 cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc ccagcgcatg
     9361 cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg gtttgataac
     9421 taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca ggctttcatc
     9481 ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc gggttttgca
     9541 cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt tggcaaactg
     9601 ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat
     9661 tgcctccagc aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca atcccacttt
     9721 gaccttcgcc actttctcgc ccggcatatc ggcaagtttg aggatcagat cgtccggatc
     9781 gccattacac agcggtgccg cacggtagtt ggctgcttgc ggcaacgtat ctgtcagttc
     9841 tgccaatgca cagcttacgc caaaggccac ggaaggcatc tgcggtagct cgcaatcgcc
     9901 tgccagccag ttatttaccc aggcaagcag cacgctttgc gcctcttccc agttttcctg
     9961 gctgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct cacgcaggca
    10021 aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc ccgcgtccat
    10081 ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt aaatttgctg
    10141 aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc ttccgtcatg
    10201 tagaacagca tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc gtcacagtcg
    10261 gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag catttcgcgg
    10321 caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt gacaaggccc
    10381 atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc tcgcgctttt
    10441 ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact
    10501 ttcgggccag tctgaccgaa gatggcatta tctgctgcga tagtcaggtc gcacatcatg
    10561 tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac cggtttcgga
    10621 caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc ggaatcatct
    10681 ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa cgctttatcg
    10741 cctgcgccag tcagaatgat cacgccgata ttgtcgtcat aacgcgcatc tgccagtgcc
    10801 tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg acgattaatg
    10861 gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcgaa accttcggag
    10921 cagtcgtgcc attcaaccgg tgcgtaaggc attgcttcat caggataaat catattgtgt
    10981 cctttagtca gaaacgcaag atctgcgcca gacttgcgat tacgccagcg ggattttccc
    11041 gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt tccgccgcca
    11101 gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat gtgcgggcgc
    11161 taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc atggcggcaa
    11221 gggttgcgcc attattgttg ctgcgcagcg ccaccagctc ccggcgttga tcgtcattga
    11281 gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac ggttctgtgc
    11341 gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcacgttgt tcagcatttt
    11401 gcagccccgg atgcccgcct tcgacaataa ccccacaaag ccccgccagc ccctggcaag
    11461 ccgccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag tcaaggatgt
    11521 tgtaactaac caaggtttta cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa
    11581 tcgccgccga accaccatga cctaggagat caacatacaa ccgtgagtag tcggcaaacg
    11641 cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa aacaccagcc
    11701 agggtaaacc tagttttccg tgttttgcct gcgcgtgcag gatcataaat ggcttacctg
    11761 cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca tttcaatcac
    11821 cgtggtggtt ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct gccagttttg
    11881 cggatgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg
    11941 catcagatag aaacgctcac gctcgctttg cggcgttggc aacagcgaga aaatttgccc
    12001 gccgttgttg ttcaccacaa ttaataccag cggcgcagaa acctgacgca ataacgccag
    12061 cgcgttgaga tcgtaaagcg cggagagatc gcccacaatc gccagcgtcg gtttgccgct
    12121 tgcccgctga acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg cccctcggtt
    12181 gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac gtaccaccag
    12241 gctgttacca acaaacaatt gcccctgttc aggcagatag tcgctgatgc gatgcgccag
    12301 ttgcgcttcg ccaaacgcat cacggcgggc aataaccgcc tgcattgcct gttccgccag
    12361 gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct ccagccagtc
    12421 ggcaatattg gcaattaagc gacgtccgcg atggtgtgcc ggatcaagtc gcccttcaat
    12481 gtcatcaaca atccagtact cttctggttc acagcttgcc tgccattgca ggagccgttt
    12541 ccccgtcagg ctacttccca gttgcaccac aatttgcgcc tgctgcagct cgctggtcgc
    12601 tttggcattg cctaaccaaa gatcggcaca cggcagcggc tgcccggttt gtgacagcac
    12661 atcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt tgccctcttc
    12721 cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga accagtcgcg
    12781 ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt cctgccacca
    12841 gtcacccaga cgctgttgcc agctaagccc ggtatcgtcc atttcgccat acagcggttc
    12901 agcaaacggg cagttgatat ggactccccc cgcatgaagc gtaccgagag cgtggtcgat
    12961 ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg aaatactgtg
    13021 cgtggggtga gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc cgcagtcaat
    13081 tagctccggc gggcgatcgg cggttaagag aatcagtttt tctccggtta acccggcttc
    13141 aatcagtgcc ggatagagat ttgccaccgc cgtgccggag gtcacaatca ccgccaccgg
    13201 ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct catcgaaatg
    13261 ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg tagaacgcga
    13321 gcctggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc
    13381 cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca atattgcggc
    13441 taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat gcgactcatt
    13501 actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt tcctgccact
    13561 cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc acgacattgc
    13621 cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgttgtaat gagagatagc
    13681 ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata aactgtcgcg
    13741 ccagatcgcg cggtaagcca gcaactgctg ccgttggcag caactgatgt aaacagatca
    13801 catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc actttacgca
    13861 gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt aatcgttggc
    13921 agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc atcagccact
    13981 ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc tactgttccc gccagcgctt
    14041 cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag
    14101 cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt cgacgactgg
    14161 cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt gcccgagcga
    14221 gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg attaattgcg
    14281 tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt aacccaggca
    14341 agggcttgat actcaccagt gtggcgataa attcttttgc ctgaatcgca tcgtgctgaa
    14401 gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag cgtcgccatt
    14461 ccaggcgggg taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc ccccaaatgc
    14521 gtaagtcagc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa
    14581 aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt tgccagtaga
    14641 attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg tctttgagag
    14701 ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc
    14761 gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc tgttaaaagc
    14821 ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac
    14881 ggcgtgccag caacagtcct agtaccagcc cgacggccgc gcccacacca attccttgcc
    14941 agggtttttc gtggacgtag tcatcagcac gataaacagc ctgcttcgcc cgatagtaat
    15001 aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt tctgcatgcg
    15061 ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc acctcttcca
    15121 gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac tgattagaca
    15181 ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagagtt gttgattacg
    15241 cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca tagacctccg
    15301 tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc gccccgaggt
    15361 aaacaggctt atcaggccag tgatgcgtac atgtttcgag tgttttactc atcagctgct
    15421 gccccacttt ttcaccacgc aacgcttcac tgacaatcac ccgacctata acgaccggct
    15481 caagatcatc atcacttttc agaatcctcg catacgccac cagttcatca tttttccaac
    15541 cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga taagggcaat
    15601 tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg
    15661 ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt tagatgtatg
    15721 ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta atttttttag
    15781 gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg cttaacctgc
    15841 aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc
    15901 tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt caccttcatg
    15961 gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc
    16021 aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc gcgctgcgga
    16081 ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct ggcgaaattc
    16141 tcgatgatgg cctgcgcaaa gtaaccgctt atccgctgga acacccactg gaatgttatg
    16201 gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg
    16261 ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca atcacgctgg
    16321 aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca ggtaaagcgc
    16381 tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct aaaggtgtcg
    16441 atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc aatagtcgcg
    16501 gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc ggcaagctaa
    16561 tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg ttacgtgaat
    16621 gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt aacgtttaac
    16681 tctgcttatt attaaaaaca gggtgaaact tgccctgtta tagcaacccg cgctacatat
    16741 atccacccac acaggcatat tgagcgattt ttgatattta ttcatctcct tacgcactat
    16801 attcagagca ataagttgcc tcttttcatc ttgattcagc acaacctttg caactaaaag
    16861 gagcccatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac ccaactcagt
    16921 cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt aaaaaaaaca
    16981 gcgttatccg gagatgaaga aagtattgag ttactgcata atattgcgtt aggttatgat
    17041 gaatttggaa aaaaagctgg gtaatgctgc caacttactg atttagtg
//
LOCUS       DAZPDE010000084        16604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPDE010000084 DAZPDE010000000
VERSION     DAZPDE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.084"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            180..332
                     /gene="mokC"
                     /locus_tag="ACHU0J_002416"
     CDS             180..332
                     /gene="mokC"
                     /locus_tag="ACHU0J_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1217077.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(436..1566)
                     /gene="dnaJ"
                     /locus_tag="ACHU0J_002417"
     CDS             complement(436..1566)
                     /gene="dnaJ"
                     /locus_tag="ACHU0J_002417"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1217078.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1655..3571)
                     /gene="dnaK"
                     /locus_tag="ACHU0J_002418"
     CDS             complement(1655..3571)
                     /gene="dnaK"
                     /locus_tag="ACHU0J_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1217079.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3948..4352
                     /locus_tag="ACHU0J_002419"
     CDS             3948..4352
                     /locus_tag="ACHU0J_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1217080.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4378..5091
                     /gene="msyB"
                     /locus_tag="ACHU0J_002420"
     CDS             4378..5091
                     /gene="msyB"
                     /locus_tag="ACHU0J_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1217081.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5240..5806
                     /gene="satP"
                     /locus_tag="ACHU0J_002421"
     CDS             5240..5806
                     /gene="satP"
                     /locus_tag="ACHU0J_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1217082.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5841..6428)
                     /gene="mog"
                     /locus_tag="ACHU0J_002422"
     CDS             complement(5841..6428)
                     /gene="mog"
                     /locus_tag="ACHU0J_002422"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1217083.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6543..7496)
                     /gene="tal"
                     /locus_tag="ACHU0J_002423"
     CDS             complement(6543..7496)
                     /gene="tal"
                     /locus_tag="ACHU0J_002423"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1217084.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7775..9205
                     /locus_tag="ACHU0J_002424"
                     /pseudo
     CDS             7775..9205
                     /locus_tag="ACHU0J_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9275..10051
                     /gene="yaaA"
                     /locus_tag="ACHU0J_002425"
     CDS             9275..10051
                     /gene="yaaA"
                     /locus_tag="ACHU0J_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1217085.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10204..10500)
                     /locus_tag="ACHU0J_002426"
     CDS             complement(10204..10500)
                     /locus_tag="ACHU0J_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1217086.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10714..12000)
                     /gene="thrC"
                     /locus_tag="ACHU0J_002427"
     CDS             complement(10714..12000)
                     /gene="thrC"
                     /locus_tag="ACHU0J_002427"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1217087.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(12001..12933)
                     /gene="thrB"
                     /locus_tag="ACHU0J_002428"
     CDS             complement(12001..12933)
                     /gene="thrB"
                     /locus_tag="ACHU0J_002428"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1217088.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12935..15397)
                     /gene="thrA"
                     /locus_tag="ACHU0J_002429"
     CDS             complement(12935..15397)
                     /gene="thrA"
                     /locus_tag="ACHU0J_002429"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1217089.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15423..15542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15478..15543)
                     /gene="thrL"
                     /locus_tag="ACHU0J_002430"
     CDS             complement(15478..15543)
                     /gene="thrL"
                     /locus_tag="ACHU0J_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1217090.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15757..16443)
                     /locus_tag="ACHU0J_002431"
     CDS             complement(15757..16443)
                     /locus_tag="ACHU0J_002431"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1217091.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3842 a         4422 c         4118 g         4222 t
ORIGIN      
        1 tgggttaaag agagcgatat aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta
       61 gctgaacggc agatagagaa aagccccgag tgatatttta ccatcaaccc gaggcctcct
      121 atatgctgaa cacatgtaga gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa
      181 tgaagcagca taaggcgatg attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg
      241 cggcgctggt aacgagaaaa gacctctgtg aggttcacat ccgaactggc cagacggagg
      301 ttgctgtttt cacggcttac gaatccgagt aagagcaacg gcggggagtg atccccgcca
      361 ctttacaggt gctcgcatat cttcaacgca ccctattttt acccaggcct gcccacgggc
      421 aggcttttgg ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa
      481 gctctttgag cgcgggctgt tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc
      541 ttgcagcagc tgtttctgct tttcgttcag gcctaccggt gtttcgacga caacgcggca
      601 cagcaaatca ccctgtgcgc caccgcggac agacttgacg cctttaccgc gcatacggaa
      661 tagcttaccg gtctgggttt cgccaggcac tttcagtttg acgcgaccat caagggtcgg
      721 tacttcgatt tcgccaccca gcgccgccat agcgaagttg atcgggactt cgcaatacag
      781 gttgttgcct tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc
      841 gcctgccggt gcgccatgct cgcccgcttc accttcgccc gcaagacgga tgcggtctcc
      901 agtgtccacc cctgccggga ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg
      961 accatgacat ttgttgcacg gatctttgat cagcgtaccg cggccctgac agtgtggaca
     1021 ggtctgctgt acagcgaaga atccctggcg catctgcacc tgaccagaac catgacaggt
     1081 cggacaggtc tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc
     1141 ttccagagtc ggaatgcgga tctctttggt cacgccacgt acagcttctt cgagggtgag
     1201 ctccatgtta tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc
     1261 aaaaatatcg ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc
     1321 accgccgccc atgcctcctt gctcaaacgc agcatgacca tactgatcgt atgccgcacg
     1381 tttttgcgag tcggtcagaa cttcataagc ttccttgatc tctttaaatt tcgcctcggc
     1441 ctctttgtca ccctggttac ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt
     1501 tctgatttca cgctcttccg ctgttttgga aacgcctaaa atctcgtaat aatcttgctt
     1561 agccatcttt tttaaattgc ccctagatga atgcacgggc ggagaggaaa ttccccttcg
     1621 cccgtgtcag tataattacc cgtttatagg gcgattattt tttgtctttg acttcttcaa
     1681 attcagcgtc gacaacatcg tcatctttcg cgttgtttgc agaagcatca gcaccggcag
     1741 tctgctgctg ggcatgttgc tgctgggcga tttccatcag tttctgggaa acctgtgcca
     1801 gttcctgcat tttcgcttcg atagcggctt tgtcttcacc tttcagagca gtttccagtg
     1861 cagtcagcgc agactcgata gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt
     1921 caacctgctt acgggtgctg tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct
     1981 cttcaaactt acggtcagct tcggcgttag cttctgcgtc gcgtaccatt ttctggattt
     2041 catcttcgtt cagaccagaa gaagccttga tggtgatctt ctgctcttta ccgctgtttt
     2101 tatctttcgc ggaaacgtgc aggataccgt cagcatcgat atcgaaggta acttcgatct
     2161 gcggcatgcc gcgcggtgcc gggttgatac catctaggtt gaactgaccc agagatttgt
     2221 tatcagccgc acgtttacgt tcaccctgca gcacatggat ggttaccgca gactggttgt
     2281 cttcagcggt agagaacacc tggctgtgct tggtcgggat agtggtgttt ttcgcgatca
     2341 gcgtcgtcat cacaccgccc atggtttcga tacccagaga cagcggggta acgtccagca
     2401 gcagtacgtc ttttacgtca ccagtcagaa caccaccctg aacagcagca ccgattgcta
     2461 cagcttcgtc cgggttaacg tctttacgcg gctctttacc aaagaactca gcaactttct
     2521 tctgaaccat tggcatacga gtctgaccac caacgaggat aacgtcgtcg atatcagata
     2581 cggacaggcc agcatcctgc agtgcaactt tcagcggctc aatggaacgg tttaccagat
     2641 cttcaaccag gctttccagt ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg
     2701 tcgcgtctgc agtgatgtat ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga
     2761 ttttcgcttt ttctgccgct tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt
     2821 caatgccctg atctttcttg aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt
     2881 cttcaccacc caggtgggta tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt
     2941 caacttcgtc gatttcgata atagaaatat cgaaagtacc accccccagg tcataaaccg
     3001 cgatagtacg gttgccagtg cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt
     3061 cgttgatgat acgttttact tccagaccag cgatacggcc tgcgtctttg gttgcctgac
     3121 gctgagcatc gttaaagtat gccggtacgg tgataacagc ttcagttacc ggttcaccca
     3181 ggtaatcttc agcggttttc ttcatttttt tcagcacttc agcagaaatc tgcggcggtg
     3241 ccattttctg gcctttaact tcgacccatg cgtcgccgtt atcagcagca ataattttga
     3301 acggcatgat ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc
     3361 gtttaatcgc aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta gccggctgac
     3421 caaccagagt ttcaccatcc tgggtatagg caatgataga aggcgtggtg cgatcgcctt
     3481 cggcgttctc cagcacgcga ggaatggtgc catccataat cgctacacaa gagttggtag
     3541 tacccaggtc gataccaatt attttaccca tctaaacgtc tccactatat attcggtcat
     3601 catgtggttg tgagtctgta ataggggcga aacgtctggt ttcaactgcc caatttcatt
     3661 tttttgcaga ctcactcact gcggttgact actaaatggg gtcgtaaccc acgtcatcaa
     3721 gggggagatg caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa
     3781 gacgtaaaat tgacagcaat tttattcata taaagaatga atcgcagcca aggcgttcat
     3841 caaaaaattg taataaaaag aaaagattac gtgcctgaat cttctcttta tcagcagtaa
     3901 actagtgggt attcatcccc ctacctcttc ccactaagag aatccttatg aaatccgttt
     3961 ttacgatttc cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg
     4021 accataaact cctcggcgtc attgcaatgc cgcgtaacga aaccaacgat ctggcgctga
     4081 aacttcctgt ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat ggcgatttac
     4141 agttaagtgg tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag agcctgaata
     4201 ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg
     4261 acaataaacg ctgcgtctca aaaatcacct tttccggtca tacggtgaac tcatcggata
     4321 tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaagcc acaaaaaatg
     4381 aatgttaatt acctgaatga ttcagatctg gattttcttc agcattgtag tgaggaacag
     4441 ttggcaaatt tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg cctctccagc
     4501 atactgatgc gcaacgaact gtttaaatcg atggaagggc atcccgagca acatcgccgc
     4561 aactggcagc tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa
     4621 ctgcgcggac acggtaaatt gtatcgggcc attttgctcg atgtttcaaa gcgattgaag
     4681 ctgaaagccg acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt
     4741 ctgcgtaata cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc
     4801 gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg
     4861 gcaaaaggtg tgtcgcattt gctttccagc caactgaccc gcattttacg cacccacgca
     4921 gcaatgagcg tacttgggca tggtttgctg cgcggcgcgg ggctgggagg ccctgtgggc
     4981 gcagcattaa atggggttaa agcggtcagc ggcagcacct atcgcgtgac gattccagcc
     5041 gtactgcaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa
     5101 ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta
     5161 ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga
     5221 tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct
     5281 gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt atttcgctct
     5341 ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg
     5401 tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc
     5461 tttctggctg acgctggttg cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc
     5521 aaatgcacag ttccttggtg tctacctggg tctgtggggc gtatttacgc tgtttatgtt
     5581 cttcggcact ctgaaaggcg cgcgcgtcct gcaattcgtt ttctttagcc tgaccgtgct
     5641 gtttgccctg ctggcgatcg gtaacattgc cggtaatgcc gcgatcatcc actttgccgg
     5701 ctggattggt ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa
     5761 cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt cttgagatcc
     5821 ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat
     5881 gcagcaacca cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac
     5941 ggtacgctgg caaaaatacc atgcaccaca acgttaccct cagcgtcctt cacaccttcc
     6001 agcgtctctt taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga
     6061 atcacgccca cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc
     6121 atctgttcac caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt
     6181 acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca
     6241 tccaccagct cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt
     6301 tccagttcaa acggcgtggt tagcgccgat gtcagccatt cttccagcgc tggaatgcct
     6361 ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa
     6421 gtattcatca agatttttcc gtttaaacaa tctcgttaaa cagaatgata cactgcgaag
     6481 ggagtgacag acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat
     6541 gattacagca gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg
     6601 ataccttccg ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg
     6661 gactcagtga tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg
     6721 atagcccctt cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtca
     6781 cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt
     6841 tcataaccgt gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct
     6901 tctgccggag cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca
     6961 acaaacggcg agatcaggaa cacgcccgcc tccgcacaag cacgagcctg agcgaaggag
     7021 aacagcaggg tcaggttaca gttgatacct tctttttcca gctgttctgc agcacggata
     7081 ccctgccagg tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg
     7141 tagagtttga tcaggcgttt tgctttcgca attgacgctt cagtgtcata ggaaagacgc
     7201 gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag accaatattt
     7261 actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc
     7321 gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga
     7381 atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc
     7441 ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata
     7501 gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg
     7561 acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt
     7621 tctgacactg cgttgatatc gcccaccatt tttatacaaa acctcatgta tgctacgcag
     7681 aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg
     7741 aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa
     7801 cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac
     7861 ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa
     7921 tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc
     7981 ggcgcgcgtg ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc
     8041 aggtgccgtc ttttggatgt gggttgctgc gtttatcggc atggcgacct cgtttgccga
     8101 atgttccctt gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc
     8161 ggcatggtat atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt
     8221 tttgctcatc gcctacggca taattttcag cggagttcag gcgaacgccg ttgcccgagc
     8281 cctgagtttt tcttttgatt ttcctccgct ggtgacgggc attattctcg ctgtctttgc
     8341 tctgctggca atcactcgtg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc
     8401 gttgatggcg ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca
     8461 acttccccac gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg
     8521 cggcgcggcg ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt
     8581 ttccaatgag gtgggaatgg gatcgacgcc aaacgcggca gcggcagctg catcctggcc
     8641 tccgcatccg gcagcacaag ggattgtcca gatgattggc atttttatcg acaccctggt
     8701 catctgtacg gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc
     8761 gctggaaggt attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc
     8821 tgaatttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat
     8881 ttatgccgaa aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt
     8941 gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagccttc cgctgatgtg
     9001 gcaactggca gatatcataa tggcctgcat ggctattacc aatttgaccg ccattttact
     9061 gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt
     9121 gcgcccggtg tttgatccgt tgcgttatcc ggatatcggt cgccagcttt ctccggacgc
     9181 gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc
     9241 taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa
     9301 aacgcttgat taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga
     9361 caattcccag cagttgatcc atgaagcgcg gaaactgacg cctccgcaga ttagcacgct
     9421 gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc
     9481 agatttcacg ccggcgaatg cccgccaggc gattctggcg tttaaaggtg atgtctacac
     9541 cggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg
     9601 aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct
     9661 ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga
     9721 tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa
     9781 tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat
     9841 caagccggtg ttcctcgatg agaagaacgg caaatttaag atcatcagct tctacgctaa
     9901 gaaagcacgc ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca
     9961 actgactggt tttaatagcg aaggttactt ctttgatgaa gattcctcca gcaatggcga
    10021 actggtgttt aaacgctacg agcagcgtta agtttgtagt caatgccgga tgcggcgtga
    10081 acgccttatc cggcctacaa aagcatgcag attcaatata ttgcagaatt aacgtaggcc
    10141 tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc cggcatttgt
    10201 catttagcgg tgatgtttgc ctggaccatg accgccgtga tgatcatgag gagctttctt
    10261 atggtggcgc ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc gccattcata
    10321 atgttgtttc caccagccgt ggtcgcgcca gtgacctcca tcccagtaat agccacgatt
    10381 atcacgatcg cctatctgta attttactga cgggactaac gtaatttccg cagcctgtgc
    10441 tgccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac
    10501 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
    10561 cgacggttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gataatttct
    10621 cctttttccc tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag
    10681 caaacccggc ctgattgaga taatgaatag attttactga tgattcatca tcaatttacg
    10741 caacgcagaa aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag cacgttctgc
    10801 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
    10861 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag
    10921 cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt ttaactcacg
    10981 cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca
    11041 gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat
    11101 cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
    11161 tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgaatga
    11221 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag
    11281 ctggttgcgc gcctcctgcg gcagctgcgc aacagcttca aagtagtagc aaatctgcgc
    11341 cagcaaacgg ctgatgttaa tcgagttagc cgagtttaac cccagcgcca ctttcagttc
    11401 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
    11461 aacagtttcg atattgccgc ccaacgtaca gaacagtttt tcttgcagtg gactgatttt
    11521 gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag catgagccac
    11581 tgcagctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat catccgcaat
    11641 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
    11701 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc
    11761 aaacgccgcg cgcacgcgct cttccaggat ttcctgtggg atttcatcac caataaacgc
    11821 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
    11881 caggctgaat tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg
    11941 ggttacggct tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt agagtttcat
    12001 ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc
    12061 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
    12121 gtcacataga gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat
    12181 ttccgcgacc gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc
    12241 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
    12301 aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
    12361 taaaatagcc cgggcttctg ccgtcgagac tttaattccc ggatacgcca gcacccacag
    12421 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
    12481 cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc
    12541 ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc cgcagtgttc
    12601 attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat
    12661 cggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct cctggcaaaa
    12721 acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa
    12781 gcgtccgagt ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
    12841 caatgcacca tcaacgggtg ccaccgccgc cccgagcaca tcaaacccga cgctcatatt
    12901 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
    12961 tacgtagcag atcggcaaag acaccggcag cggtaacgtc attgcccgca ccatatccgc
    13021 gcagcaccaa cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat
    13081 ttttcacttt gaacagcgga tcattcccat ccacttcggc aatcttcacg cggcagacac
    13141 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
    13201 cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac
    13261 cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt
    13321 cacgtcccgt ttcacgagcg agaatcaata gtttacgcgc cacatccata ccagaaagat
    13381 catctcgcgg gtccggttcg gtataaccca tttcccgcgc cagcgtggtc gcctcggaga
    13441 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg
    13501 agaacttcac caattcatca cctgcattga gcagattttg caggttctca ataaccggta
    13561 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcataac
    13621 gcaactgatg atagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt
    13681 ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt
    13741 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat
    13801 taaacggctc tttggcttgt gcaagttctt cctgccagtt ttccagatta aggccatgca
    13861 cactagtgag cagagccttc gagttggcaa cgccgcagac acgtaagtcg atatgtttat
    13921 tcttcagcca gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
    13981 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
    14041 gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc
    14101 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
    14161 agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
    14221 tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
    14281 cccgttcagc tcgcacacag tcgctttgtg gaacgcagaa actgatactg tattcggaag
    14341 atgattgcgt aatcagcacc acggaaatac gggcgcgtga cattgcagca aagacgcgcg
    14401 ccgccatgcc aaccatccct ttcatccccg ggccggaaac gctgaacatt gccatgttat
    14461 tcagattgga aatgcccttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
    14521 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga
    14581 tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
    14641 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
    14701 agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca
    14761 ccgccgcgga gtagtcggaa ccgttacgtc caagcaccac cagttcgcct ttttcattac
    14821 cggcggtgaa acctgccatc aacaccatgt gatcagccgg aatgcggctt gccgcaatac
    14881 ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
    14941 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
    15001 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
    15061 gccccaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc tgatcgacga
    15121 aagttttcaa ttgcgccagc gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt
    15181 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
    15241 tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca
    15301 cctgcccctg cctggcattg ctttccagaa tatcggcaac acgcagaaaa cgttctgcat
    15361 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
    15421 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
    15481 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
    15541 catggatgtt gtgtactctg taatttttat ctgtttgtgc gctatgccta tattggttaa
    15601 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
    15661 aagctgctat cagacactct ttttttaatc cacacagaga catattgccc gttgcagtca
    15721 gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat ttttttcaat
    15781 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
    15841 ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgccat
    15901 gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg
    15961 ttgttgtatt aatgttgcta attgatagca atagaccatc accgcctgcc ccagattgag
    16021 cgaaggataa tccgccacca tcggcacacc agtaagcacg tcagccaacg ctaactcttc
    16081 gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg gcatggctca tccatgaaga
    16141 tttttcctct aacagcggca ccagttctac tggcgtggcg tagtaatgat atttcgcccg
    16201 actacgcgca gtggtggcga cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg
    16261 gaaaacttta atattatcaa taatatcacc agatccatgt gcgacccagc gggtggctgg
    16321 ctccaggtgt gcctgactat cgacaatccg cagttcgcta aaacccatcg ttttcattgc
    16381 ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accaggataa tcgttatacg
    16441 catattgcca ctcttcttga ccaaataacc gcgaacaggg tgatcactgt caacttatta
    16501 cgcggtgcga atttacaaat tcttaacgta agccgcagaa aaagcccttt acttagctta
    16561 aaaaaggcta aactattttc tgactgcact aacggtttaa ttgt
//
LOCUS       DAZPDE010000085        16445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPDE010000085 DAZPDE010000000
VERSION     DAZPDE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.085"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHU0J_002432"
     CDS             <1..228
                     /locus_tag="ACHU0J_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217092.1"
                     /translation="DGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQE
                     TLFSSKSHGKYALFPIFTASWRATRIKNKGI"
     gene            451..948
                     /gene="ubiC"
                     /locus_tag="ACHU0J_002433"
     CDS             451..948
                     /gene="ubiC"
                     /locus_tag="ACHU0J_002433"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1217093.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            961..1833
                     /gene="ubiA"
                     /locus_tag="ACHU0J_002434"
     CDS             961..1833
                     /gene="ubiA"
                     /locus_tag="ACHU0J_002434"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1217094.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1988..4411)
                     /gene="plsB"
                     /locus_tag="ACHU0J_002435"
     CDS             complement(1988..4411)
                     /gene="plsB"
                     /locus_tag="ACHU0J_002435"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1217095.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4582..4950
                     /gene="dgkA"
                     /locus_tag="ACHU0J_002436"
     CDS             4582..4950
                     /gene="dgkA"
                     /locus_tag="ACHU0J_002436"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1217096.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5060..5668
                     /gene="lexA"
                     /locus_tag="ACHU0J_002437"
     CDS             5060..5668
                     /gene="lexA"
                     /locus_tag="ACHU0J_002437"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1217097.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5741..7066
                     /gene="dinF"
                     /locus_tag="ACHU0J_002438"
     CDS             5741..7066
                     /gene="dinF"
                     /locus_tag="ACHU0J_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1217098.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7182..7391
                     /locus_tag="ACHU0J_002439"
     CDS             7182..7391
                     /locus_tag="ACHU0J_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1217099.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7433..7948)
                     /gene="zur"
                     /locus_tag="ACHU0J_002440"
     CDS             complement(7433..7948)
                     /gene="zur"
                     /locus_tag="ACHU0J_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1217100.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8266..8520
                     /gene="yjbL"
                     /locus_tag="ACHU0J_002441"
     CDS             8266..8520
                     /gene="yjbL"
                     /locus_tag="ACHU0J_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1217101.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8544..9251
                     /locus_tag="ACHU0J_002442"
     CDS             8544..9251
                     /locus_tag="ACHU0J_002442"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1217102.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9614..10651
                     /gene="dusA"
                     /locus_tag="ACHU0J_002443"
     CDS             9614..10651
                     /gene="dusA"
                     /locus_tag="ACHU0J_002443"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1217103.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10785..11027
                     /gene="pspG"
                     /locus_tag="ACHU0J_002444"
     CDS             10785..11027
                     /gene="pspG"
                     /locus_tag="ACHU0J_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1217104.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11193..12176)
                     /locus_tag="ACHU0J_002445"
     CDS             complement(11193..12176)
                     /locus_tag="ACHU0J_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1217105.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12259..13674
                     /gene="dnaB"
                     /locus_tag="ACHU0J_002446"
     CDS             12259..13674
                     /gene="dnaB"
                     /locus_tag="ACHU0J_002446"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1217106.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13727..14806
                     /gene="alr"
                     /locus_tag="ACHU0J_002447"
     CDS             13727..14806
                     /gene="alr"
                     /locus_tag="ACHU0J_002447"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1217107.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15059..16252
                     /gene="tyrB"
                     /locus_tag="ACHU0J_002448"
     CDS             15059..16252
                     /gene="tyrB"
                     /locus_tag="ACHU0J_002448"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1217108.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3918 a         3903 c         4358 g         4266 t
ORIGIN      
        1 gatgggcctg ttatggtggt aagctcccag cgcttacatg atatgttgaa tcctacaaaa
       61 gataccaatt ggaattccac gtgtatctat aaatccagac ataagatgtt gcctattaat
      121 cttactcagg aaacactttt cagctccaaa tctcatggta aatatgcgct tttcccaatt
      181 tttactgcga gttggcgagc tactcgtata aagaataaag gtatttaagt aaaggaaaac
      241 atcaccgttc ctagcatcat gggcggtgat gcccctatgg ttgccctcgc cagcacgggc
      301 atcggcaaag cgtaaggttc aacagcgttt taccacttca tgcgattgtt gcgtttttgt
      361 tgcgcattag atcacttaat ttgctttaca tctcccgtaa acacttttct gcgatacaat
      421 gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt
      481 gcgctgcgct attttaaaga gatccctgcc ctggatccgc aactgctcga ctggctgttg
      541 ctggaggatt ccatgacaaa acgttttgaa cagcagggaa aaacggtgag tgtgacgatg
      601 atccgcgagg ggtttgtcga gcagaatgaa atccccgaag aactgccgct gctgccgaaa
      661 gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc
      721 ggtcgtaccg tcgttcctgt atcaacatta agcgggccgg aactagcgtt acaaaaattg
      781 ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cactaacccg ggattttatc
      841 gagataggcc gtgatgccgg actgtggggg cgacgttccc gcctgcgatt aagcggtaaa
      901 ccgctgttgc taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat
      961 atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat
     1021 aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca
     1081 ccgggcgttc ctcagctctg gatcatggcg gtgtttgtcg cgggtgtctg gctgatgcgc
     1141 gctgctggtt gcgtggtgaa tgattatgct gatcgcaagt ttgatggtca tgttaagcgc
     1201 acggcgaacc gaccacttcc cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt
     1261 gtagtgctgg tactgatttc gtttttactg gttctgacgc tgaatacgat gaccattctg
     1321 ttgtcgattg ccgctctggc attggcgtgg gtgtacccgt ttatgaagcg gtatacccat
     1381 ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgccgct
     1441 gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg
     1501 gtggcttacg acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt
     1561 aaatccacgg caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt
     1621 ggcgtactgg cactgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac
     1681 tggtcaattg tggtggctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc
     1741 gagcgtgaag cctgctttaa agcatttatg aataataact atgttggtct ggtactgttt
     1801 ttaggactgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc
     1861 ggctttcttc tgggttgcct gatgcgttgc gcttctcagg cctacccggt gaatcgcaat
     1921 ttattgaatt tgcaggtcat ggtaggccgg ataaggcgtt ttcgccgcat ccggcaattc
     1981 tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa
     2041 tcaactccgc cagcaactga taaaccttca tcgtttctgc tggttcggca tcgccgctat
     2101 cgctgatata cccttcatca cgcagcgtca gcaccagaga actgaacacc gccttgtcga
     2161 agaactccgg cgcgttgatg ccgtgcagca cggagagacg ttgcgcaacg gtgcggctct
     2221 ctttctccag cgtaccgcgg ttgatcgacg ggttggcact caacaaccag aaggtgatgg
     2281 cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagggtg cgagaatgcg
     2341 ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat
     2401 ttgccagcgc atcaataacg tccggcaact cgtcgcgatc ccagcgcagg aacagctccg
     2461 ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc
     2521 gatgctgggt gacaattgcc gccatcagcg aaggcagcac caacatatgc gcaatgttgt
     2581 tgcgatagta ggtcatcagc accgcttgct cgcgtggcag aatgatgatg tcgccgatag
     2641 tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc
     2701 tggctgaagg aacggtagag tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc
     2761 agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgat gccagtagcg
     2821 cagtacagca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat
     2881 cggcagcaat attattgacc gtcggcgtta accaggccgg acgcaccgct tcgatgggat
     2941 cgatagattc acgccagtct ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt
     3001 caccgaagtt gacgtaaccc tgaccgagat tacgcagctt gcttaaaccg cgcagcatct
     3061 gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt
     3121 ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacgc gtgccgccac
     3181 gcagcatcgc ctgaatggtc atcgacagcg taccggtttt cggatccagc aaacgccccg
     3241 tgcgggaacg accgccttcc acgaagtact cgacggaata accacggctg aacagttcgc
     3301 cgagatactc acggaaaacg gtggaataaa gtttattgcc tttaaaagta cggcgaataa
     3361 agaacgcccc cagacggcgg aaaatcggcc cggccggcca gaagttcagg ttgatcccgg
     3421 cggcgatatg cggcggcacc agtccctggt ggtacagcac gtaagaaagt agcaggtagt
     3481 ccatgtgact gcggtggcaa ggcacataca ccagctcatg accgtcgtgg gccagctggc
     3541 gaacgcgctc ggcgttatgg acgttgatgc cctgataaaa tcggttccag gtgaagccca
     3601 gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc tcttccatca
     3661 gcgcaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgc gcttcatctt
     3721 ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt aaacagatcc tgacgagcag
     3781 gcagacgtgg gcctacggca gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg
     3841 ccagtttctg agcgatagtt ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaca
     3901 ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg aaaaatttct
     3961 gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc cccggcgcgc
     4021 gaccaaacat caccgatact ggcaccatct gcacatccag atttgggttg ctacggtgca
     4081 aatcgagata gtcgtggaaa agcttaatag actcttcttt cggcgtgtaa taggtgaata
     4141 cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg atttccagcg
     4201 gctctaacgg gtcaggcaaa tcatgtgcca gacactgggc gcgcaacgtc agcaaatctg
     4261 ctttcgagtt gtacggtaaa acgtacataa ttggacgaga ggtatccagc cccagttccg
     4321 gggcaggatc tgccggaata gacttgcttt ttaccaggat gcttaatggt aaattcagta
     4381 atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg gttaataatg
     4441 caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc gctcatgttt
     4501 cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg tacactgata ttgacgctca
     4561 taatgtaaaa aggttctttc aatggccaat aatacaactg gattcacccg aattatcaaa
     4621 gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc ggcattccgt
     4681 caggaaggcg tagcggtatt gttggcggtg gtcatcgcct gctggctgga tgtggacgca
     4741 attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga aatcctcaat
     4801 agcgccatcg aagcagtggt tgaccgaatt ggctctgaat accatgagct ttccggacgc
     4861 gcaaaagata tgggatccgc cgcggtgctg attgccatta tcgtcgccgt gattacctgg
     4921 tgcatcctgt tatggtcgca ttttggataa cccttccaga tttcgataaa tctctggttt
     4981 attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag tataactgta
     5041 tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct
     5101 catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg
     5161 tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg
     5221 cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga
     5281 agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc aacagcatat
     5341 tgaaggtcat tatcaggtcg atccttcctt gttcaagccg aatgctgatt tcctgctgcg
     5401 cgtcagcggg atgtcgatga aagatatcgg cattatggat ggcgacttgc tggcagtgca
     5461 taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaagttac
     5521 cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt
     5581 taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaagggc tggcggttgg
     5641 ggttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc cgcttggcgt
     5701 cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc tcacttcatc
     5761 tgataaagca ctctggcatc tcgccttacc catgattttc tccaatatca ccgttccgtt
     5821 gctgggactg gtcgatacgg cggtaattgg tcatcttgat agtccggttt atttgggcgg
     5881 cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt ttttacgcat
     5941 gagcaccacc gggctgactg cgcaggctta tggtgccaaa aatcctcagg cattagcccg
     6001 tgcgctggtg caaccgttgc tgttggcgtt gggggccggg gcgttaattg cgctgttgcg
     6061 tacgccgatt atcgatctgg cgctgcatat tgttggcggt agtgaagcgg tcctggaaca
     6121 ggcgcggcgc tttcttgaaa tccgctggtt aagcgctccg gcgtcgctgg cgaatctggt
     6181 attactcggt tggttactcg gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt
     6241 cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc atatgaacgt
     6301 gcagggcgcg gcgctggcga cggttattgc ggaatatgca acattgctga ttggtctgct
     6361 aatggtgcgt aaaatcctca aactacgcgg aatttccggc gaaatgctga aaactgcctg
     6421 gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat atcatgctgc gttcgctgtt
     6481 gttgcaactc tgtttcggcg cgatcaccgt acttggtgcg cgactgggga gtgacattat
     6541 cgctgttaac gcggttctga tgacgctact cacctttacc gcctatgcgc ttgatggttt
     6601 tgcctacgcg gttgaagcgc actccggtca ggcatacggt gcgcgcgacg gtagccagtt
     6661 gctggatgta tggcgggcag cgtgccgcca gtcggggatt gtagcgttac tattttcggt
     6721 ggtttatttg ctggcagggg aacacatcat tgcgttgctg acgtcgttaa cccagattca
     6781 gcagctggct gaccgctatc ttatctggca ggtgattttg ccgttggttg gcgtctggtg
     6841 ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc gccgaaatgc gtaacagtat
     6901 ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc tgggtaatca
     6961 tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc gggctttctc tggcggctat
     7021 ctggcggcgt cactggcgca acggtacctg gtttgccgca acgtgacggt taaaaattct
     7081 gaataaatta tcctaagcca aattgctgac tacacttaat ctcacgttca gaagaaaagt
     7141 gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa gatgaagccg
     7201 gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg
     7261 atgatatgac gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt
     7321 atggttatca gaaagatcag gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat
     7381 atcgctggta attaatccct cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg
     7441 gtttcttttt cacctgcaca gagtgatcat gctggcactg ttcaggatga cgacacgctt
     7501 ccacttccac gcatgccgca cagagcccat gtgcttcaat cacattatgc cgcagggcaa
     7561 accccatttt tgccgccagc gtatgcataa tgtcttccac gccttctgca cactcttctt
     7621 tcaccgcgcc gcagcgatcg caaataaaca tggctgacgt atgcgtgggc tggtcgaaca
     7681 gatgacagag cacataactg ttggtggatt ccaccttatg cacaaaacct tgctcaagca
     7741 gaaaatccag cgcgcgataa accgttggcg gcttggcttg cggttcagct tcgcgcagta
     7801 aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg cgcaacactt
     7861 ccaggcgctg tggggtcagg cgcacattac gctgcgcgca gattttttca gcctgcgcta
     7921 ataactcctg cgttgtggtc ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc
     7981 ttactttacc atgttccagg aaaaacaccg agaaccctta ttgttgccgt aatgttgatt
     8041 ttctgttttg taggtaaggt gttgtgttgc cttatcgtac cattatcaac acgataataa
     8101 ttaataattc atttttaaat aaattgcttc aagttaactc gctatttatt taactgggtt
     8161 taaacgcata cctgtagttc agaaattatg agcgattttt gatagtgacc agatggcaaa
     8221 agaattataa ggaggacacg gtacgacaaa aaaggtagat gaatcatgtt gaagataata
     8281 cccggtgcaa ctggatactt taataaaaca ctcaattcaa atcaattcga aaataaagat
     8341 gcaaccaaag ataaattgga taatagaggt tcaatcaaag gtaaattaaa taatatatat
     8401 ggtaaatcta ttgattatgc agcgctgcgt catcgggata taattattgc aaaaatagat
     8461 ttgtttattc aacgaatcac gcataattta tggcatgcac gtaaaaaaat gtgtttttaa
     8521 ttgagcacat aaatgatctc aaaatgtggg tcaataagta tactgacgat tgcactgatg
     8581 aggatttaaa cgatcgtgac tttattgcat cagttgttga ccgggctatt tttcatttcg
     8641 cgattaatag tatatgtaat cctggggata ataaagatgc aatgcccatt gaacaatgta
     8701 cttttgatgt agaaactaag aatggccttc cctccacggt tcagctattt tatgaggaat
     8761 ctaaggataa tgaaccttta gcgaatatac attttcaagc aataggttct ggttttttaa
     8821 cgtttgttaa tgcctgccag gaacatgatg acaacagctt aaaattattt gcttcgctgt
     8881 taatttcact ttcatattct agtgcctacg cagatttatc agaaacagtg tatattaatg
     8941 aaaataatga gagctacctg aaagctcagt ttgaaaaatt atatcaacgt gatatgaaga
     9001 agtacctggg agagatgaag cgtctggcag atgggggaga aatgaatttt gatggctatc
     9061 tggataagat gtcacatctg gtgaatgaag gaacgctcga tcctgatatt ttaagcaaaa
     9121 tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc gtttgaccca acctcaaagg
     9181 aagagattaa aatacttaca gacacttcta aattaattta tgacttgttc ggggttaaat
     9241 cggagaaata atatgtgaag ttcttcgata gtatggaagg cattatataa aaggacccaa
     9301 tatttattgg gttctttttt ctctatcaat gctattagca gggagatata tcaccagagt
     9361 ttaatgtgtg attttttatt tatcgtcgaa cctggattgt ttatcattgg ccttaacaaa
     9421 gttaacggct aataagatta tttccatcac ttcgtgagag cttcatgcct tgagaggatc
     9481 tcaattttct tttgcaatga gacaggcgct tcctgttgtt atggtatagt accccgctat
     9541 tgagcctcct gaacagtgaa gctgaataac ataaccccat gatatatcga taaaataatc
     9601 tctacatttg aaaatgcacg gtaattctga aatgcaaaaa atcaaccaaa ccagcgcaat
     9661 gcctgaaaaa actgacgttc actggagtgg tcggtttagt gttgcaccaa tgctcgactg
     9721 gacggacaga cattgccgct atttcttgcg tctgctttcc cgcaatacgt tgctttatac
     9781 cgaaatggtg accacagggg cgataattca cggtaaaggt gattatctgg cgtacagtga
     9841 agaagaacat ccggtagcgt tgcaactggg cggtagcgat ccggcggcgc tggcgcagtg
     9901 tgcaaagctg gcagaagcgc gcggatatga tgagatcaac ctgaatgtcg gctgcccgtc
     9961 tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg ggtaatgcgc agctggttgc
    10021 cgactgcgtg aaagcgatgc gcgatgtggt gtcgattccg gtgacggtga aaacgcgtat
    10081 tggcatcgac gaccaggaca gctatgaatt tctctgcgat ttcatcaata ccgtttccgg
    10141 caaaggcgag tgtgagatgt tcatcatcca cgcacgtaaa gcctggcttt cggggttaag
    10201 cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg cgtgtgtatc aactgaagcg
    10261 tgacttcccg catctgacaa tgtcgattaa cggtggtatc aagtcgctgg aagaagctaa
    10321 agcgcatttg caacatatgg atggcgtgat ggtcgggcgc gaggcgtatc agaatccggg
    10381 tattctggcg gcggtagacc gggagatctt tggttcctcg gataccgatg ccgatccggt
    10441 ggcagtagtg cgtgccatgt atccgtacat tgagcatgaa ctcagccagg ggacgtatct
    10501 cggccatatt atccggcata tgctgggttt gttccagggt attcctggcg cgcggcagtg
    10561 gcggcgttat ttaagtgaaa atgcccataa agcgggtgct gacattaacg tgctggaaca
    10621 cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc aaaaagtagt caaattcacc
    10681 acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc aatagattat ttttggcatg
    10741 attcttgtaa tgccagcaag agatttcata tttgggagag catcatgctg gaactacttt
    10801 ttgtgattgg cttttttgtc atgctgatgg tcaccggcgt ttcgttgctg ggcattatcg
    10861 ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg tatgctggca ttgatgatta
    10921 agttgctgcc gtggttacta ctggcgattg cggtggtgtg ggttattaag gcgattaaag
    10981 caccaaaaat gccgaaatat cagcgttatg accgctggcg ttactaaggg attgtgcgga
    11041 tgatcacaac ctaaggtttt atccttagaa caaaatagga attgataatc aaatctgtca
    11101 ctattgcgcc tctaacagat tcatcgtgct gtaccctaca tacagccgaa ctataaaaag
    11161 aaagggcttc ccaggtggaa gccctatttc ttttatggaa tcagcaggct ggaaccttgc
    11221 gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat ccttcagcgg atatttctgc
    11281 tgctcggcga catcgacctt aatcacaccg ctggcaatca aagagaacag ttcattactg
    11341 gcctcggtta attcctcccg cgtggtgata tagccttgca gggaagggcg tgtcacatac
    11401 aacgagcctt tttgattgag aatgcctaag ttcacaccgg taaccgcacc tgatgagttg
    11461 ccaaaactga ccattaagcc gcggcgttgc aggcaatcca gcgaccgttc ccaggtgtct
    11521 ctgcccacgg aatcgtacac tacgcgcact ttcttaccgc cggtgatctc tttcaatcgc
    11581 tcgaccagat tctcttcacg atagttaata acctgccacg cgccggcttt tagcgcgctc
    11641 tgcgcttttt gcgcggttcc tacggtgccg atgagtttcg cgcccagggc ttttgcccac
    11701 tggcaggcaa ttaagccaat gccgccagcc gctgcgtgga acaggaactg ttcatcgggt
    11761 ttaatttcat aggttttgcg cagcagataa taaaccgtta agcctttcag gaaggatgcc
    11821 gcagcttgct caaaagaaat tgccgcaggc agaatcgccg ctttatccgc attaatgtta
    11881 tgcacagagc tgtaagcgcc taacgccgac tgcgcataga ctacatgatc gccagcctta
    11941 atatgcttta caccactgcc gactttactc acgatgcctg ccgcttcggt acctaatccg
    12001 ctgggtagcg atggcggcgg gtaaaggccg ctgcggatat acgtgtcgat aaaattgatg
    12061 ccgatggctt tattttcgac ctggatttca ttctccgccg gatcggcagg agtgaactct
    12121 acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt caattcgtgt tgccatgtgt
    12181 cctccagaac gtatcgtcag ggtctgcttc atatggtaaa gtttcgaccc attctttatt
    12241 tcggtaactc cattcactat ggcaggaaat aaacccttca acaaacagca ggctgaaccc
    12301 cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc cgcactcgat cgaagcggag
    12361 cagtcggtgt tgggcggttt aatgctggat aacgaacgct gggatgatgt agccgagcgt
    12421 gtggtagcag acgattttta cacccgccca caccgtcata tctttactga aatggcgcgt
    12481 ttgcaggaaa gcggtagtcc tatcgatctg attacccttg cggaatcgtt ggaacgccag
    12541 gggcaactcg atagcgtcgg tggttttgct tatctggcag agctgtcaaa aaatacgcca
    12601 agtgcggcga acatcagtgc ttatgcggac atcgtgcgtg aacgtgccgt tgtccgtgag
    12661 atgatctcgg ttgcgaatga gattgctgaa gccggttttg atccgcaggg gcgtaccagc
    12721 gaagatctgc tggaccttgc tgaatcccgc gtctttaaaa ttgccgaaag tcgtgcaaac
    12781 aaagacgaag ggccgaagaa catcgccgat gtgctcgacg caaccgtggc gcgtattgag
    12841 cagttgtttc agcagccaca cgatggcgtt accggggtaa acaccggtta tgacgatctc
    12901 aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca tcgtcgccgc gcgtccgtcg
    12961 atgggtaaaa caacatttgc gatgaacctc gtcgaaaacg cggcaatgtt gcaggataaa
    13021 ccagtactta tcttctcgct ggagatgcca tcagaacaga tcatgatgcg ttctctggcg
    13081 tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc agctcgatga tgaagactgg
    13141 gcgcgcattt ccggcaccat gggtattttg ctcgaaaaac gcaatatcta tatcgatgac
    13201 tcttccggcc tgacgccaac ggaagtgcgt tcccgcgcac gccgtattgc ccgtgaacac
    13261 ggcggcatcg ggcttatcat gatcgactac ctgcaactga tgcgcgtacc ggcgctttcc
    13321 gataaccgta cgctggaaat tgcagaaatc tcccgctcgc tgaaagcact ggcgaaagaa
    13381 ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt ctctggaaca acgtgccgac
    13441 aaacgtccgg tcaactccga cctgcgtgaa tctggctcta tcgagcagga tgcggacttg
    13501 atcatgttta tctatcgtga tgaggtgtat cacgaaaaca gtgatttaaa aggcatcgcg
    13561 gaaattatta tcggtaaaca acgtaacggc ccaatcggga cggtacgcct gacctttaac
    13621 ggtcaatggt cgcgcttcga caactatgcg gggccgcagt acgacgacga ataataatta
    13681 ttttatgaat taggtaatta aagcaaacaa ttatcaagga acacaaatgc aagcggcaac
    13741 tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa cgtcttcgtg aactggcacc
    13801 tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat ggtcacggtc ttcttgagac
    13861 cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc cgtctcgaag aagctctgcg
    13921 actgcgtgcg gggggaatca ccaaacctgt actgttactc gaaggctttt ttgatgccag
    13981 agatctgccg acgatttctg cgcaacattt tcataccgcc gtgcataacg aagaacagct
    14041 ggctgcgctg gaagaggcta gcttggacga gccggttacc gtctggatga aactcgatac
    14101 cggtatgcac cgtctgggcg taaggccgga acaggctgag gcgttttatc atcgcctgac
    14161 ccagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc cattttgcgc gcgcggatga
    14221 accaaaatgt ggcgcaaccg agaaacaact cgctatcttt aatacctttt gcgaaggcaa
    14281 acctggtcaa cgttccattg ccgcgtcggg tggcattctg ctgtggccac agtcgcattt
    14341 tgactgggtg cgcccgggca tcattcttta tggcgtctcg ccgctggaag atcgctccac
    14401 cggtgccgat tttggctgtc agccagtgat gtcactaacc tccagcctga ttgccgtgcg
    14461 tgagcataaa gccggagagc ctgttggtta tggtggaacc tgggtaagcg aacgtgatac
    14521 ccgtcttggc gtagtcgcga tgggctatgg cgatggttat ccgcgcgccg cgccgtccgg
    14581 tacgccagtg ctggtgaacg gtcgcgaagt accgattgtc gggcgcgtgg cgatggatat
    14641 gatctgcgta gacttaggtc cacaggcgca ggacaaagcc ggggatccgg tcattttatg
    14701 gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgacg aaagtaagcg cttacgaact
    14761 tattacgcgc ctgacttcaa gggtcgcgat gaaatacgtg gattaatcgt tctgtaatat
    14821 ttgattgtct gtgccggatg cggcgtgaat gccttatccg gccaataaaa tcctaaaaat
    14881 tcaataagtt gatgttcttt catgctctta taaaggtcgt gcctctggcg gatgtacgtt
    14941 tatcattagt ctcactctgt tgctaattgc cgttcgctcc tgaacatcca ctcgatcttc
    15001 gccttcttcc ggtttattgt gttttaacca cctgcccgta aacctggaga accatcgcgt
    15061 gtttcaaaaa gttgacgcct acgctggcga cccgattctt acgcttatgg agcgttttaa
    15121 agaagaccct cgcagcgaca aagtgaattt aagtatcggt ctgtactaca acgaagacgg
    15181 aattattcca caattgaaag ccgtggcgga ggcggaagcg cgcctgaatg cgcagcccca
    15241 tggcgcttcg ctttatttac cgatggaagg gcttaacagc tatcgccatg ccattgcgcc
    15301 gctgctgttt ggtgcggacc atccggtact gcaacaacag cgcgtagcaa ccattcaaac
    15361 ccttggcggc tcaggggcat tgaaagtggg cgcggatttc ctgaaacgct acttcccgga
    15421 atcaggcgtc tgggtcagcg atcctacttg ggaaaaccac gtagcaatat tcgccggggc
    15481 tggattcgaa gtgagtactt acccctggta tgacgaagtg actaacagcg tgcgctttaa
    15541 tgacctgttg gcgacgctga aaacattacc tgcccgcagt attgtgttgc tgcatccatg
    15601 ttgccacaac ccaacgggtg ccgatctcac taatgatcag tgggatgcgg tgattgaaat
    15661 tctcaaagcc cgcgagctta tcccattcct cgatattgcc tatcaaggat ttagtgccgg
    15721 tatggaagag gatgcctacg ctattcgcgc cattgccagt gctggattac ccgctctggt
    15781 gagcaattcg ttctcgaaaa ttttctccct ttacggcgag cgcgtcggcg gactttctgt
    15841 tctgtgtgaa gatgccgaag ccgctggccg cgtactgggg caattgaaag caacagttcg
    15901 ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg gtggctgcag tgctgaatga
    15961 cgaggcattg aaagccagct ggctggcgga agtagaagag atgcgtactc gcattctggc
    16021 aatgcgtcag gaattggtga aggtattaag cacagagatg ccagaacgca atttcgatta
    16081 tctgcttaat cagcgcggca tgttcagtta taccggttta agtgccgctc aggttgaccg
    16141 actacgtgaa gaatttggtg tctatctcat cgccagcggt cgcatgtgtg tcgccgggtt
    16201 aaatacggca aatgtacaac gtgtggcaaa ggcgtttgct gcggtgatgt aatgcaggaa
    16261 agcaggctgg agttacccag cctgcagtga aattaaactg tcgtcgcttt cactatttct
    16321 ttgtagatga tttttttgat gccgttgttc tacgtgagag ataataaaac gttgtcagtt
    16381 cttttattgt taagtttatc ccaattatct ggaattcctt tccctatttt ttcgtgtgga
    16441 gtaat
//
LOCUS       DAZPDE010000086        16390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPDE010000086 DAZPDE010000000
VERSION     DAZPDE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.086"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHU0J_002449"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHU0J_002449"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1217109.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHU0J_002450"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHU0J_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1217110.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHU0J_002451"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHU0J_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1217111.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHU0J_002452"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHU0J_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1217112.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHU0J_002453"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHU0J_002453"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1217113.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACHU0J_002454"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACHU0J_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1217114.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACHU0J_002455"
     CDS             5920..6417
                     /locus_tag="ACHU0J_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1217115.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACHU0J_002456"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACHU0J_002456"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1217116.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHU0J_002457"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACHU0J_002457"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1217117.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACHU0J_002458"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACHU0J_002458"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1217118.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACHU0J_002459"
     CDS             9102..9665
                     /locus_tag="ACHU0J_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1217119.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHU0J_002460"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACHU0J_002460"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1217120.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACHU0J_002461"
     CDS             complement(10257..11237)
                     /locus_tag="ACHU0J_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1217121.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACHU0J_002462"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACHU0J_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1217122.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACHU0J_002463"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACHU0J_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1217123.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACHU0J_002464"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACHU0J_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1217124.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACHU0J_002465"
     CDS             12838..13431
                     /locus_tag="ACHU0J_002465"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1217125.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACHU0J_002466"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACHU0J_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1217126.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="ACHU0J_002467"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="ACHU0J_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="ACHU0J_002468"
     CDS             15882..16385
                     /locus_tag="ACHU0J_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1217127.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4124 g         3987 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatggat
//
LOCUS       DAZPDE010000087        16387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPDE010000087 DAZPDE010000000
VERSION     DAZPDE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.087"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHU0J_002469"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHU0J_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHU0J_002470"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHU0J_002470"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1217128.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHU0J_002471"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHU0J_002471"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1217129.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHU0J_002472"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHU0J_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHU0J_002473"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHU0J_002473"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1217130.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHU0J_002474"
     CDS             complement(5877..6218)
                     /locus_tag="ACHU0J_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1217131.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHU0J_002475"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHU0J_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1217132.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHU0J_002476"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHU0J_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1217133.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHU0J_002477"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHU0J_002477"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1217134.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHU0J_002478"
     CDS             12897..14240
                     /locus_tag="ACHU0J_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1217135.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHU0J_002479"
     CDS             complement(14302..14508)
                     /locus_tag="ACHU0J_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1217136.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHU0J_002480"
     CDS             14833..15387
                     /locus_tag="ACHU0J_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1217137.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16387)
                     /locus_tag="ACHU0J_002481"
     CDS             complement(15450..>16387)
                     /locus_tag="ACHU0J_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217138.1"
                     /translation="LPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWVS
                     NYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLRG
                     SGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQA
                     FGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGF
                     ALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSN
                     ITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3924 a         4456 c         3991 g         4016 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaaca
//
LOCUS       DAZPDE010000088        16096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPDE010000088 DAZPDE010000000
VERSION     DAZPDE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.088"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..588
                     /locus_tag="ACHU0J_002482"
     CDS             <1..588
                     /locus_tag="ACHU0J_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR1217139.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHU0J_002483"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHU0J_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR1217140.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHU0J_002484"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHU0J_002484"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR1217141.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHU0J_002485"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACHU0J_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHU0J_002486"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHU0J_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1217142.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHU0J_002487"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHU0J_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1217143.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHU0J_002488"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHU0J_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1217144.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHU0J_002489"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHU0J_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1217145.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHU0J_002490"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHU0J_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1217146.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHU0J_002491"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHU0J_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1217147.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHU0J_002492"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHU0J_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1217148.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHU0J_002493"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHU0J_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1217149.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHU0J_002494"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHU0J_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1217150.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHU0J_002495"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHU0J_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1217151.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHU0J_002496"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHU0J_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1217152.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHU0J_002497"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHU0J_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1217153.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHU0J_002498"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHU0J_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1217154.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHU0J_002499"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHU0J_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1217155.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHU0J_002500"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHU0J_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1217156.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHU0J_002501"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHU0J_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1217157.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHU0J_002502"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHU0J_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1217158.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHU0J_002503"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHU0J_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1217159.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHU0J_002504"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHU0J_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1217160.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHU0J_002505"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHU0J_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1217161.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHU0J_002506"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHU0J_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1217162.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHU0J_002507"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHU0J_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1217163.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHU0J_002508"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHU0J_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1217164.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHU0J_002509"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHU0J_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1217165.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHU0J_002510"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHU0J_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1217166.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHU0J_002511"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHU0J_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1217167.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHU0J_002512"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHU0J_002512"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1217168.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHU0J_002513"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHU0J_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1217169.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3783 c         4292 g         3880 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ttggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAZPDE010000089        15935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPDE010000089 DAZPDE010000000
VERSION     DAZPDE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.089"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            96..638
                     /locus_tag="ACHU0J_002514"
     CDS             96..638
                     /locus_tag="ACHU0J_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1217170.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            725..1927
                     /locus_tag="ACHU0J_002515"
     CDS             725..1927
                     /locus_tag="ACHU0J_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1217171.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            1991..2452
                     /locus_tag="ACHU0J_002516"
                     /pseudo
     CDS             1991..2452
                     /locus_tag="ACHU0J_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2493..3443)
                     /locus_tag="ACHU0J_002517"
     CDS             complement(2493..3443)
                     /locus_tag="ACHU0J_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1217172.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3636..4826)
                     /gene="glpC"
                     /locus_tag="ACHU0J_002518"
     CDS             complement(3636..4826)
                     /gene="glpC"
                     /locus_tag="ACHU0J_002518"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1217173.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4823..6082)
                     /gene="glpB"
                     /locus_tag="ACHU0J_002519"
     CDS             complement(4823..6082)
                     /gene="glpB"
                     /locus_tag="ACHU0J_002519"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1217174.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6072..7700)
                     /gene="glpA"
                     /locus_tag="ACHU0J_002520"
     CDS             complement(6072..7700)
                     /gene="glpA"
                     /locus_tag="ACHU0J_002520"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1217175.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7973..9331
                     /gene="glpT"
                     /locus_tag="ACHU0J_002521"
     CDS             7973..9331
                     /gene="glpT"
                     /locus_tag="ACHU0J_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1217176.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9336..10412
                     /gene="glpQ"
                     /locus_tag="ACHU0J_002522"
     CDS             9336..10412
                     /gene="glpQ"
                     /locus_tag="ACHU0J_002522"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1217177.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10454..10696)
                     /locus_tag="ACHU0J_002523"
                     /pseudo
     CDS             complement(10454..10696)
                     /locus_tag="ACHU0J_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10691..10795
                     /locus_tag="ACHU0J_002524"
                     /pseudo
     CDS             10691..10795
                     /locus_tag="ACHU0J_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10875..11525
                     /gene="inaA"
                     /locus_tag="ACHU0J_002525"
     CDS             10875..11525
                     /gene="inaA"
                     /locus_tag="ACHU0J_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR1217178.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11579..11833)
                     /gene="yfaE"
                     /locus_tag="ACHU0J_002526"
     CDS             complement(11579..11833)
                     /gene="yfaE"
                     /locus_tag="ACHU0J_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1217179.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(11833..12963)
                     /gene="nrdB"
                     /locus_tag="ACHU0J_002527"
     CDS             complement(11833..12963)
                     /gene="nrdB"
                     /locus_tag="ACHU0J_002527"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1217180.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13197..15482)
                     /gene="nrdA"
                     /locus_tag="ACHU0J_002528"
     CDS             complement(13197..15482)
                     /gene="nrdA"
                     /locus_tag="ACHU0J_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1217181.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15610..15753
                     /gene="ypaB"
                     /locus_tag="ACHU0J_002529"
     CDS             15610..15753
                     /gene="ypaB"
                     /locus_tag="ACHU0J_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1217182.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3698 a         3932 c         4343 g         3962 t
ORIGIN      
        1 tagatccctt ttttcgctca ttgaatcctc tgtgccaggc agtacaatcc cgcagctttg
       61 ggaattttga ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc
      121 tggccggaat gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg
      181 gtaaagaata caccaatatt ggtgtcggtt ttgggactga atcgacgggc ctggctttaa
      241 gcggtaactg gacacataac gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga
      301 atctgcctct cgggccgtta atggcgaccg ttggcggaaa aggcgtgtac accaacccga
      361 attacggcga tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg aaaattggca
      421 acagcttccg tttgtttggc gagtattact actctccgga ttcgctctcc agcggtattc
      481 aaagttatga ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca gtcagtattg
      541 aggcgggtta tcgctacctg aatctgtcgg gtaaagacgg taaccgcgac aacgctgtgg
      601 ctgacggcct gtatgttggg gttaacgcca gtttctgatc ccttccggcg cggcattcag
      661 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
      721 aaccatgtta aaagtggaaa tgctatccac cggggatgaa gtgttatacg ggcaaatcgt
      781 tgacactaac gctgcctggc tggccgattt tttctttcat caggggctgc cattatctcg
      841 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
      901 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc
      961 actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga
     1021 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     1081 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     1141 cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     1201 gatggtcgag cacgaaatcc tgccgcgcct gcgcgagcgt ttttctttac cacagccgcc
     1261 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     1321 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgatcatcga
     1381 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     1441 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     1501 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     1561 tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtgattcc
     1621 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggccaacca
     1681 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca actttgcgct
     1741 agccacgcca gacggcactt tcgctctgcg tgtgcgtttc agcactacac gctacagcct
     1801 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctacgcc gttggttaaa
     1861 cggccaggac atcgctagtg agcatggctg gattgaggtt gttgagtcca tgactttatc
     1921 cgtctgattt cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     1981 tttgtccaac cctccctacg gtgagcgtgg tgttggggcc agtgtggcac gggctgcgcg
     2041 ctggggacgc attgagaatt acatggcgca agtcaacgat tcgctttgtc tgttagtgca
     2101 ggtggaaagt aaaacggcac tggataacct ggacgagatc ctcgacgtcg aagggattga
     2161 tggcgtgttt attggaccag cggatctttc tgcgtcgttg ggctacccgg ataacgccgg
     2221 gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa
     2281 agcggctggt tttctggctg tggctcctga tatggcgcag caatgcctgg cgtggggagc
     2341 gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg atcaacgact
     2401 ggcgatgttt aaatcaggca aaaatgggcc acgcataaaa ggtagttatt gatatcaaag
     2461 gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc cagatcggct
     2521 tcgcttagct gggtggccgc gagcacttgg tcacgatcaa tgccctgttc cagcatgcgc
     2581 aaagcaattt taatggcttg ttcatgcata ccttcctgca aaccttctaa tttaccttgc
     2641 tgcaaaccta ctaatttacc ttcttgccag ccaatttgat gtccttcctg ccgtagccgt
     2701 tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat ctcctgaata
     2761 aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag tgtttgtagc
     2821 tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac catgccgatt
     2881 aaatcgtggt cgcgaatatg cttttgaatc agttccagca gagctatacg ccgatgttgc
     2941 atgatctcat cgtcaggtac gatggtgata tccaccaacg gaaaagcttc ggtgtacaac
     3001 tgccgggcaa gttgcggatc gtcaaactca tccagccagt taagtgagta cgggtagggc
     3061 gatgtttcgc catgataaaa cagcaatggc accaccagcg gaactctgtc atagccttta
     3121 tctaggtgac tctgcatggc ggcagtggca tagcgcattg gccgaaaagc catattcttt
     3181 tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc gctggtttcc
     3241 acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa actagtgggt
     3301 tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg gttctggtaa atgtatttcg
     3361 agaaaatccc gtgcggtttc gggtgtgaat ataaaggttt taaataccgc atcatgcggg
     3421 gaggaggttg ttgattcggt catggttgtc cgtcacctga tatttatgat gacgcgacat
     3481 tacggttatc ccaggccttt gacatctgcc agtttcagcc tttgcgagac gttttcagag
     3541 gaattttcac tgcgttgcaa cgcatacatt gggcacggaa tcgtgtacgc aaaaatgccc
     3601 ggtaaccaca tgcttaccgg gcatcagaag ggagtttaag ccagcgcctg ggccagtagc
     3661 gtaatcggat gttcgcagcg aagacttgtg gacatctcaa tctgccattt acaggtttcg
     3721 cagtcggtga ccaccagatc tgcgccgctt tcttctatct ggcggaacag tggtgcgccg
     3781 atggcttgtg aggtggggta gttctctttt ttgaaaccgt aagtacccgc aataccgcag
     3841 cactgggaat ccagcaccgt taactcaagc cccgggatgt tacgcaacag ctccagggtg
     3901 tagagcgtcc agcccatttt ttccatatgg cacggagtgt gataaaccac tttcagcggc
     3961 agcggtttca gcggtaacgt tttgccttcg tccagcttgc gccacagcca gcgggttgcc
     4021 agttcgatat gatcgcgcaa gcctttgttg tcgacattca gcacttccgg gtattcgtcg
     4081 cgcagggcaa atgtacaggt tgaggaggtg gcaatcaccg gaatgccttt tactcccaca
     4141 gcttcgcgga tcgactctac attcgtaatt gcctgtttgc gtgctttatc ggtaaagccg
     4201 ttggcgatta gcggtacgcc gcagcatttt tctttgctga gcagttgtac accggtaccc
     4261 attgcgttga gcactttaat taaatcttta cctaactgcg gatggttgta gttaacgaag
     4321 cagccgtgaa agaaagcgac ctggtcttta tattgtgctt gctgagccgc tatgctgcga
     4381 taccagcgac ggaacgtgcc gaaggagtat ttcggtagcg tgcgacgatg atcgattttt
     4441 aacgccgcat caagcagctg ccgcaccggt ttcagcgagg tagcggtgtt gacgattggt
     4501 gcgaacggcg tggaaacgct acccatcagg tcggtatgac tcaacacaaa attacgcagc
     4561 gacgggcgcg tggtgtcata tttcgcccgc gcgcgctgga taatatcgcc aatcttcaca
     4621 tcggacgggc aggcgacttc acaacgtttg cagttgatgc aatatttcag cgcctcgtca
     4681 tacagtgcgc catctttcaa acgcagacgc tcgccatccg gcccggcttg ttttggccct
     4741 ggataaccgg gattcacccg gctcaccggg caggcggtgg tgcagacggt gcacttaatg
     4801 cagttttcga agctggtgtc gttcattgtt ggcctcctgc gcgttgggca atctgttgag
     4861 cggcatgtaa agcactgacg gcacaaacac cgccgccgca tccctgggcg atgggatcaa
     4921 atccgcccag caccgaaccg atggcaaaca ggttctcaat ggtttgccct gcctgcgacg
     4981 ggcgtagcgt ctcatcagtg gttacaccga actgctgcca cggttgcggc gcaaaaaaat
     5041 cgcccttata ccattcaccc cgcgtggcgg tttgtagcac atcaaggccg agaatcggct
     5101 cgcgaatgcc gttacgttcc gctactagtc cgccactaaa gaaactgccg ctggcgagaa
     5161 ccgcgaaacg tggacgtagc ggaatatcgg cgtgattgcg ggtccagatt tcattcacta
     5221 cgccattttt acaggtcact tttttcactt catcgcccgg catccacact ccaccttggc
     5281 gcacaaactg gcgctgtaac tggttttgca gacgaatgcc cagcacggaa ggcggcagcg
     5341 ttggcaaaag catcagtgaa caaggtagtt tttcattcaa ccaacgccac agtttgtcat
     5401 cggccaggcc gaagcaggcg ggcatcagga tcatttcgca ggtattggcg acaggaataa
     5461 gcgcatcaag taacagcggc cagttttctt cattatcaag gaaacgggcg atattcaccg
     5521 cgcgaaattc ggtggcgtta ttgcgcagga catccagttc cggcagctct atttctgcgg
     5581 tttcaaccga aagatccagt tcgcgcaacg aagccgccgc aagatgcgcc tggaaatcca
     5641 tcagcccgct tatccccact acacatatct tcttcgcggg tagcggccag acgggaactt
     5701 ctggcgaact taaccaggtt gcacgcaaag tgccgagcgg cgtaatccgc tggtgagcca
     5761 gttctacgct gccctgcaat tgcgcaccgc tttcagcgat taatgcctgc gcctggcaag
     5821 cgagatcgag cacgcgttgt ggcccgagaa gagagtaagg atgtgcaggt gcctgctgac
     5881 gcaaagattc cagtccactg tgaatgtctg ccacgggttg accatctggc agatggctca
     5941 gcaaatccag cgaaccggat gagaaatgca acgcgctttg accacgcgtg acaatggcac
     6001 agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa tccggcgagg ccgccgccca
     6061 taatgacagt atcaaagcgc atctttctgc tccttctcca gaccacacag tccctgataa
     6121 acccagcggg taaattcgct ttcgcgcagt gcgtctcccc aggcgatggg ttgcacgcct
     6181 ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag attgcgcgga cgtcgtgacg
     6241 ttaaaccgtt gcagcagtcc ggcagcgcgg caggcgcaga gttcgccctg gcaggtgccc
     6301 atccccacac gggtacgacg gcgtaaatcc agcaggctat taacgtttaa attttctacc
     6361 gcgtactgca cttcacccgc agttaccgct tcgcactcac ataccaggct acggtgtaga
     6421 cggccttcgc tcagccaggc aggcgtgcga tcgccatgac gataaaccgc agaaccgcgc
     6481 agcggggcag gcagggagat gactttacgc aaggtaactt cagcgggttc ttgtgaacca
     6541 ggcagtgcca gatcggcagt cgtacagggg cgcgtgttgc ccagtttgcg gcataccgcg
     6601 tcggtagccc attcagccat cagccgatag gtcatcagtt tgccaccggt gatggtgata
     6661 aatccgtcca gaccatcgcg ttcagcatgg tcgagcagca cgatgccacg gctgacgtta
     6721 cgtccgctcg ggtcgtcatc gctggcaacc agcgggcgca cgccagaata ggcccgcaaa
     6781 atgcgcgttt tcgccatcac gggggccagt ttttcccctt cacgcagcag aatatcaacc
     6841 tcttctgccg tcactcgatt atcgtcaatc tcgttgtaat caatacgtaa agaggtggta
     6901 ccaatcagcg aaatggtatc gccaggcacc agaatatcgg cgtcggaagg tttacggcag
     6961 cggttgatca catgctggtt aatgcggtga tccatgatca gcagcgatcc tttcgccggg
     7021 aacatgcgaa tgcgcagatc ggcatattcg gcaatgtgtt gcccccagat cccagcggca
     7081 ttaaccacga caggtgcatg aagggcctga gtttcgccgg tgagatggtt acgtacacga
     7141 acaccgcaca ccgtcgcgcc ttcacgaatc agccccgtga cttcatgagc ggtaaggata
     7201 acggcaccgt gttctttggc atccagcatg tttgctgcgg tcagacgaaa tggatcaacg
     7261 gtgccatccg gaactttcac cgcgccaatc agtgccgggt taacggcagg ttcgataatg
     7321 cgcgcttgct gcgggtctat agcttctgcg ctgatccctg cttcttcgca ggcgcgaata
     7381 aaagtggcct ggaatgagag gtcatcttcc ggcagggtga taaacaggcc gttggttggt
     7441 tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt cactaatgca ttcgcgggcc
     7501 gattccgcat cggttactgc atagcgcgca ccgctgtgca gcaggccgtg gttacgcccg
     7561 gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca cgcgcagccc gcgcagggca
     7621 cagtcgcggg caatcccggc tcccgttgcg ccgccgccaa tgataatcac gtcacttgat
     7681 tgcgagtcgc gagttttcat tgttatccct ctgaagttcg ttttttacca tttagccata
     7741 gtaaaaacat gaattgtttg atttcgcgca tattcgctca tgattcgaaa gtaaaacgtg
     7801 atttcatgcg tcattttgaa cattttgtaa atcttattta ataatgtgtg cggcaattca
     7861 catttaattt atgaatgttt tcttaacatc gcggcaactc aagaaacggc aggttctctc
     7921 actgaatcag gctgttaatc ataaataaga ccacgggcca cggaggctat caatgttgag
     7981 tatttttaaa ccagcgccac acaaagcgcg cttacctgcc gcggagatcg atccgactta
     8041 tcgtcgattg cgctggcaaa ttttcctggg gatattcttt ggctatgcgg cttactattt
     8101 ggttcgtaag aactttgcgc ttgctatgcc ttatctggtt gagcagggat tctcacgcgg
     8161 tgatttaggt tttgcccttt cggggatctc gattgcttat ggattttcga aattcatcat
     8221 gggttcggta tcggatcgct cgaatccgcg cgttttcctg cccgcaggtt tgattctggc
     8281 ggcggcagtg atgttgttta tgggctttgt gccatgggcg acgtcgagca ttgcggtgat
     8341 gtttgtactg ttgttcctct gcggttggtt ccaggggatg gggtggccgc cgtgtggtcg
     8401 tactatggtg cactggtggt cgcagaaaga acgtggcggc attgtgtcag tgtggaactg
     8461 tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc ctgctgggga tggcctggtt
     8521 caatgactgg catgcggcgc tctatatgcc tgctttctgc gccattctgg tggcattatt
     8581 cgcctttgcg atgatgcgcg ataccccgca atcctgtggc ttgccgccga tcgaagagta
     8641 caaaaatgat tatccggacg actataacga aaaagcggaa caggagctga ccgcgaagca
     8701 aatcttcatg cagtacgtgc tgccgaacaa actgctgtgg tacatcgcca tcgccaacgt
     8761 gttcgtttat ctgctgcgtt acggcatcct cgactggtca ccgacttatc tgaaagaggt
     8821 taagcatttc gcgctggata aatcctcctg ggcctacttc ttctatgagt atgcgggtat
     8881 tccgggcacc ctgctgtgcg gctggatgtc ggacaaagtc ttccgtggta accgtggggc
     8941 aaccggcgtc ttctttatga cactggtgac catcgcgact atcgtttact ggatgaaccc
     9001 ggcaggtaac ccaaccgtcg atatgatttg tatgattgtt atcggcttcc tgatctacgg
     9061 tcctgtgatg ctgatcggtc tgcatgcgct ggaactggca ccgaaaaaag cggcaggtac
     9121 ggcagcgggc tttaccgggc tgtttggtta cctgggcggt tcggtggcgg cgagcgcgat
     9181 tgttggctac accgtggact tcttcggctg ggatggcggc tttatggtaa tgattggcgg
     9241 cagcattctg gcggttatct tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatga
     9301 acaattactg caaaaacgca acggaggcta atggcatgaa attgaagctg aaaaacctta
     9361 gcatggcgat catgatgagc actatagtca tgggaagcag tgcaatggcg gcggacagca
     9421 acgaaaaaat agtcatcgcc catcgcggtg ccagtggata tttgccggag catacgctgc
     9481 cagcaaaagc gatggcgtat gcgcagggag cggattatct ggaacaggat ttggtgatga
     9541 ccaaagacga ccatctggtt gttctgcatg accattatct cgatcgtgtt actgatgttg
     9601 ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta ctacgcgata gatttcacgc
     9661 tggatgaaat taagtcgctg aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag
     9721 tacagactta tccggggcgt ttcccaatgg gtaagtccga cttccgggtg cacacctttg
     9781 aagaagagat tgaatttgtt caggggttaa atcactctac cgggaaaaat atcggtatct
     9841 atccagaaat caaagcgccg tggttccatc atcaggaagg gaaggatatt gcggcaaaaa
     9901 cgctggaagt gctgaagaaa tatggttaca ccggtaaaga cgataaagtt tatttgcaat
     9961 gttttgatgc tgatgagctg aagcgtatta agaatgagct ggaacccaaa atgggcatgg
    10021 agctcaatct ggtacagctg attgcctata ccgactggaa tgaaacgcag cagaaacagc
    10081 cggatggaag ctgggttaat tacaactacg actggatgtt taagccggga gccatgaaac
    10141 aggtggcgga atatgcagat ggtattggtc cggattacca tatgttgatt gaggagacat
    10201 cgcagccggg taatatcaaa ctcactggca tggtgcaaga tgcccagcag aataaactgg
    10261 tagtgcatcc ttataccgtg cggtcagata aactgcctga atacactcct gatgtgaatc
    10321 agttatatga tgctctgtat aacaaagcgg gtgtaaatgg gctgtttact gatttccctg
    10381 ataaggcagt aaaatttctt aataaagagt aatgttactg caggatggcg ttcatgcgcc
    10441 atcctgtctc atatcaaatt tttccaatcg ccactaattg ttccatgcac atttgtagta
    10501 aaaacagtgg actgccttct accggctttt ctttagtcag caacgaaatc tgtcgataga
    10561 aggttggttc gtgaggaacg gaacacaatt cacctgcaat gcttttcagc gttaactctg
    10621 gttgtaaggc gatgcctaat ccctgtcgaa taaaattcat cgctgtatcc gactgattaa
    10681 attcacattt tatctgctgg gcgcactatt agtggtagtg gctggtggaa tctatgcgct
    10741 gcaaatgaag ggcaaaagcg gtgtcacacc tgtagcggca aaagaaattc attaatacga
    10801 cacgtttcat taagattttc ctcaggtaac cacctatagt cattgtcgtt aataaaaatg
    10861 acaggcgata gggtatggca gtttcagcaa agtacgacga atttaatcac tggtgggcaa
    10921 cagagggcga ctgggttgaa gagcctaact atcgccgtaa tggtatgagc ggggtgcaat
    10981 gcgtcgagcg caacggcaaa aagctgtatg taaagcgcat gacgcatcat ctgtttcatt
    11041 cagtacgtta tccgttcggc cgaccaacga ttgtccgtga ggttgcggta attaaagaac
    11101 tggaacgggc cggtgtcatc gtgccgaaga tcgtttttgg tgaagcggtg aaaattgagg
    11161 gtgaatggcg cgcgttgctg gtgactgaag atatggcggg gttcatcagc attgctgact
    11221 ggtatgcccg gcatgcagta tcgccttatt ctgacgaagt acggcaagcc atgttgaaag
    11281 cagtggcact ggcgtttaag aaaatgcata gcgttaatcg tcagcatggc tgttgctatg
    11341 ttcgccatat ctatgtgaaa acagaaggta aggcagaagc tggttttctt gatctggaga
    11401 aaagtcgtcg ccgcttgcgt cgcgataaag cgattaatca tgacttccgc cagctggaaa
    11461 aatatctgga gccgatccct aaagcggact gggagcaggt gaaagcgtat tactacgcca
    11521 tgtaatcgtt gtaggccaga taatacgcgt aagcgtcgta tctggcgcta gtacccgatc
    11581 acaactcgat ttcaatatcg ccttttgccc ggcaacaaca gggcaaaatt tcccccggct
    11641 gaataaaggc taacggttcg gcaatccagt ccacctgacc tgcgaccagt cgggtgcgac
    11701 aggagccgca gtaaccttcg cggcactggt actcaaccgc cacattgtgg gattccagcg
    11761 ccgccagaag ggaagggtgt tcatcctggc acagcagttg tgtgccagtg atgcgcaggg
    11821 taacgcgggc catcagagct ggaagttact caaatcgtcg gtgtccactt ccgcgtcaat
    11881 ctgcccgacc agataagaac tgacttccac ttcctgcgga gcaacctgca cgttatcaga
    11941 caccagccag gtgttgatcc acgggatcgg gttggagcgc gtctggaacg gcagatccaa
    12001 accgactgcc tgcatgcgga tattggtgat gtattcaacg tactggcaga gaatatcttt
    12061 attcagacca atcatcgaac cgtcacggaa cagataatcc gcccagtctt tctcttgctg
    12121 tgccgcctgg acaaacaggt catagcactc ctgcttacat tcttcggcaa tttccgccat
    12181 ctccggatca tccgcgccgc tgcgcagcag attcagcata tgctgagtac cggtcaggtg
    12241 cagggcttca tcgcgggcaa tcaggcgaat aatcttggcg ttaccttcca tcagttcgcg
    12301 ttctgcaaag gcaaaggaac aagcaaagct gacgtagaag cggatcgctt ccagcgcgtt
    12361 aacgctcatc aggcagagat acagtttttt cttcagctcg cgcaggctaa cggttacggt
    12421 tttaccgtta acggtgtggg tgccttcgcc cagaaggtgc cagtagctgg tcatttcgat
    12481 cagttcatcg taatagctgg agatcccttc cgcacgtttc tggatctgct cgttggtgac
    12541 gatatcgtca aacacaacag acggatcgtt aacgatatta cgaatgatat gagtatagga
    12601 acgggaatga atcgtttctg agaacgccca ggtttcgacc caggtttcca gttccggaat
    12661 agaaataagc ggcaatagcg ccacgttcgg gctacgaccc tgaatggaat ccagcagcgt
    12721 ctgatatttc aggttgctga taaagatgtg tttttcgtgc tccggcagcg cctggtaatc
    12781 tatacggtcg cgggagacgt caacttcttc cggacgccag aagaaagaga gctgcttttc
    12841 gatcagcttt tcgaagatgt catatttttg ctgatcgtag cgagccacgt tgaccggctg
    12901 accaaagaac atcggttctt tgagctgatc atttttcgtc tgtgaaaagg tggtatatgc
    12961 catgagtgtg tcctgttggg agtttaatgc cggataaggc attttacgcc gcatcctgtg
    13021 cctgatgcga cgctggcgcg tcttatcagg cctacaaatc cgagccgtag gccggataag
    13081 gcgttcacgc tgcatccggc acccggagcc tgatgcgacg ctggcgcgtc ttatcaggcc
    13141 tacaaaccga gccgtaggcc ggataaggcg tttacgccgc atccggcatc tcaatatcag
    13201 atcttacatg cgccgctttc gcagccatcg tcctggattg acggtaccag atcgtcctgc
    13261 gcgtcttcag cgccgtcacg ggtgttctga taatacagcg ttttgacccc gaatttgtag
    13321 gcggtgagca ggtctttcag caactgctgc atcggcactt ttcctgacgg gaaacgtgac
    13381 ggatcgtagt tggtgttagc agagatcgac tgatcgataa atttctgcat gatacctacc
    13441 agttgcaggt agccatcgtt acccggcatt tcccacagca gctcataggc gtcgtgcagg
    13501 tgctcgtagt ccggcaccac ctggcgcaaa ataccgtctt tcgacgcttt gatgctgacg
    13561 taaccgcgcg gcggttcaat accgttagtg gcgttagaga tctgcgaaga ggtctcggac
    13621 ggcatcaggg cagaaagcgt ggagttacgc aggccgtgcg ttttgattga ttcacgcaat
    13681 gcttcccagt cgtaatgcag cggctcatta gcgatggcat ccagatcttt cttataggta
    13741 tcgatcggca ggatcccttt cgcgtaagtg gtttcgttaa accacgggca cgcgccttgc
    13801 tctttagcca gctcattaga cgctttcagc aggtaatact gaatcgcttc gaaggtttta
    13861 tgcgtcaggt tgttggcgct gccgtcggag tagcgtttac cgtgcttcgc cagatagtaa
    13921 gcgaagttaa tcacaccaat acccagcgta cgacgcccca tcgctccacg tttggcggcc
    13981 gggatcgggt aatcctgata atccagtagc gcgtcaagtg cacgaaccgc cagaatcgcc
    14041 agctcttcca gttcatccag gctattaatt gcgcccaggt tgaaagcaga cagcgtacac
    14101 agcgcgattt caccgttctc gtcgttgacg tcgttcagcg gtttggtcgg cagggcaatt
    14161 tctaggcaca ggttagactg gcgaactggc gcgatgttcg gatcaaacgg actatgggta
    14221 ttacagtggt caacgttctg aatatagata cgaccggtag acgcacgttc ctgcatcatc
    14281 agcgagaaca gctcaacggc tttcacacgc tgcttgcgga tgctgtcgtc tttctcatat
    14341 ttggtataca ggcgttcaaa ctcttcctga tcggcgaaga acgcgtcgta cagccccggt
    14401 acgtcggacg ggctgaacag ggtgatatct tcacctttca gcaggcgggt atacatcagt
    14461 ttgttgattt gtaccccgta gtccatatga cgcacgcggt tgccttcaac cccacggttg
    14521 tttttcaaca ccagcaggct ttccacttcc agatgccaca tcgggtagaa cagcgttgcc
    14581 gcaccgccgc gcacaccgcc ctgagagcag gatttcaccg ctgtctggaa atgtttgtag
    14641 aacgggatac agccggtatg gaacgcttcg ccaccgcgaa tcgggctgcc cagcgcacga
    14701 atacgtccgg cgttgatgcc gatcccggca cgctgggaaa catatttcac aatcgcgctg
    14761 gaggtggcgt tgatggaatc caggctgtca ccgcactcga tcagtacgca ggagctgaac
    14821 tgacgagtcg gggtacgcac gccggacatg attggcgtcg gcagcgaaat tttaaatgtg
    14881 gaaaccgcgt cataaaaacg cttcacgtat tgcaggcgcg tttcacgcgg gtagttcgag
    14941 aacaggcacg cggcaacaag aatataaagg aactgggcgc tttcgtagat ttcgccggtc
    15001 acgcggttct gtactagata tttgccttcc agctgcttaa cggcagcata agagaaggtc
    15061 atatcacggt cgtgatcgat aaacgtgtcc atctgcttga actcttcttc cgtgtagtct
    15121 tccagcagat gattatcgta tttgcccatc tcgaccattt tcaccacgtg gtcgtacagc
    15181 gcaggcggct caaactggcc gtaggctttt ttacgcaggt ggaagatcgc caggcgcgcg
    15241 gcgagatact gataatccgg cgcatcacgg gagatcaggt ctgcggcagc cttgataata
    15301 gtttcgtgga tgtcagaggt cttgataccg tcataaaact gaatgtggga gcgcagctcg
    15361 acctgggaaa tcgaaacgtt atgcagtcct tctgccgccc aatccagaac gcgatggatt
    15421 ttgtcgagat tgatgcgctc tgtgctaccg tcgcgctttg tcaccagcag attctgattc
    15481 atgtatgtcg tacctgtttt tggaattctt tccgcaatag tttcatgcag actacctgta
    15541 gtaagggaag ctgcttagaa cactatatat agggggtatg tttgggattc actgcaagat
    15601 agtgtgaaaa tgaccctctt gcaagtgcat aactttgtgg ataactcagg aaggaaaaag
    15661 tggctttcgc gcaccttagg tcagacaagg tgtccgggaa agtcaatggg aagagaaaaa
    15721 tttgttaaaa ataactgttc ggcgatttct tgaacggcag aataggttgg ataaattgat
    15781 atagatggtt tattaatctt tttgctaatg attgcaaaaa ttgatctaag cacgagtcat
    15841 cacataatct gatgtatcag ttgtggtcaa tatataagcg aatgtaagcg attgttgatg
    15901 tcgaaatggt tgttttctat tcggttattg ttaaa
//
LOCUS       DAZPDE010000090        15903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPDE010000090 DAZPDE010000000
VERSION     DAZPDE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.090"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            448..1506
                     /gene="citC"
                     /locus_tag="ACHU0J_002530"
     CDS             448..1506
                     /gene="citC"
                     /locus_tag="ACHU0J_002530"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1217183.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1521..1817
                     /gene="citD"
                     /locus_tag="ACHU0J_002531"
     CDS             1521..1817
                     /gene="citD"
                     /locus_tag="ACHU0J_002531"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1217184.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1814..2722
                     /gene="citE"
                     /locus_tag="ACHU0J_002532"
     CDS             1814..2722
                     /gene="citE"
                     /locus_tag="ACHU0J_002532"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1217185.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2733..4265
                     /gene="citF"
                     /locus_tag="ACHU0J_002533"
     CDS             2733..4265
                     /gene="citF"
                     /locus_tag="ACHU0J_002533"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1217186.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4269..4819
                     /gene="citX"
                     /locus_tag="ACHU0J_002534"
                     /pseudo
     CDS             4269..4819
                     /gene="citX"
                     /locus_tag="ACHU0J_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4794..5672
                     /gene="citG"
                     /locus_tag="ACHU0J_002535"
     CDS             4794..5672
                     /gene="citG"
                     /locus_tag="ACHU0J_002535"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1217187.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5723..7185
                     /gene="citT"
                     /locus_tag="ACHU0J_002536"
                     /pseudo
     CDS             5723..7185
                     /gene="citT"
                     /locus_tag="ACHU0J_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7299..8105
                     /gene="rna"
                     /locus_tag="ACHU0J_002537"
     CDS             7299..8105
                     /gene="rna"
                     /locus_tag="ACHU0J_002537"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1217188.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8334..8744
                     /gene="rnk"
                     /locus_tag="ACHU0J_002538"
     CDS             8334..8744
                     /gene="rnk"
                     /locus_tag="ACHU0J_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1217189.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8882..8971
                     /gene="yldA"
                     /locus_tag="ACHU0J_002539"
     CDS             8882..8971
                     /gene="yldA"
                     /locus_tag="ACHU0J_002539"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1217190.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8975..10184)
                     /locus_tag="ACHU0J_002540"
                     /pseudo
     CDS             complement(8975..10184)
                     /locus_tag="ACHU0J_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10405..10833
                     /gene="uspG"
                     /locus_tag="ACHU0J_002541"
     CDS             10405..10833
                     /gene="uspG"
                     /locus_tag="ACHU0J_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1217191.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10954..12519)
                     /gene="ahpF"
                     /locus_tag="ACHU0J_002542"
     CDS             complement(10954..12519)
                     /gene="ahpF"
                     /locus_tag="ACHU0J_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1217192.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12764..13327)
                     /gene="ahpC"
                     /locus_tag="ACHU0J_002543"
     CDS             complement(12764..13327)
                     /gene="ahpC"
                     /locus_tag="ACHU0J_002543"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1217193.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13699..14445
                     /gene="dsbG"
                     /locus_tag="ACHU0J_002544"
     CDS             13699..14445
                     /gene="dsbG"
                     /locus_tag="ACHU0J_002544"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR1217194.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14655..15557
                     /locus_tag="ACHU0J_002545"
     CDS             14655..15557
                     /locus_tag="ACHU0J_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1217195.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15704..15903
                     /locus_tag="ACHU0J_002546"
                     /pseudo
     CDS             15704..15903
                     /locus_tag="ACHU0J_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3950 a         4104 c         3847 g         4002 t
ORIGIN      
        1 aagatacgca gcggaaatgc cagtctttgg aaaaatgcaa actgtttatt ctcgttaagc
       61 tgcaacattg ttttccttgc gggttgtccc gagcctcttt gacggatgta attaaaatgt
      121 tgaatcaaaa tacatagata ttaattatga tcgtggcaaa ttttattcat taaatcaatt
      181 aaatcactta aacctgcgag tcatttcgta attacattaa ttaaaaaact taaaaccatt
      241 aaataaataa aaaaccacta actctatgtg aaataaatca aaatttcacg ccgaaatact
      301 ccttaggatg tatagcaaaa ggagaaaaag atatacctcg atcacccccc tttctcccaa
      361 gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa atcgataaca
      421 acatgcacct tcaggatact atttattatg ttcggcaatg atattttcac ccgcgtaaaa
      481 cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc
      541 gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct tatcgcgtgc
      601 ggtggaattg ccggaaatat tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa
      661 ggactggcgc tgacattagc cactgaactg ataaacctcg cctatgagcg gcacagcacg
      721 catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg cggtttttcc
      781 acgctgacct gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa
      841 cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg ctgcattgtg
      901 atgaacgcca atccctttac gaatggtcac cgttatctga ttcaacaagc tgcggcacag
      961 tgcgactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc ctatgaagac
     1021 cggctggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt gcatcgcggc
     1081 tcagaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc
     1141 attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct cgctcccgcg
     1201 cttggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac
     1261 aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcagcgcc catcgaactg
     1321 gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa
     1381 ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat
     1441 tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc
     1501 gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt
     1561 ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca
     1621 atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg
     1681 cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca
     1741 ttttacatgc acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat
     1801 gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag
     1861 catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca tctacccggc
     1921 tgatgccctg atgtttgacc tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg
     1981 ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg
     2041 tgtcaacgcg ctggattccg aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg
     2101 tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atattgaaaa
     2161 agagatcctg cgtatagaaa aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc
     2221 ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg cttccgagcg
     2281 tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc
     2341 cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc
     2401 gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca
     2461 agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca
     2521 gattgatctg ctgcacaacc tctacgcacc gacccagaaa gaagtggatc acgcccaccg
     2581 cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg
     2641 caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact
     2701 ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac
     2761 gacaagaacg ggtagcggcc tggaatcgtc gcactgaatg cgatcttgcc gctttccaga
     2821 actcaccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc
     2881 tggaagaagc gattcgtcgc tcaggtttac aggacggcat gacggtttcc ttccatcacg
     2941 ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct
     3001 ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg ctggtagaac
     3061 acattcgcca gggcgtggtt acccgcattt atacctctgg cctacgcggt ccactggcgg
     3121 aagagatctc ccgtggtctg ctggcggaac cggtgcagat ccactctcac ggcggtcgtg
     3181 tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc gtcccgtcct
     3241 gtgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc tccctcggct
     3301 atgcaatggt tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc
     3361 cttatccgca taatccggca agcattgagc aagatcaggt tgatttgatc gtcaaagtta
     3421 atcgcgttgg cgatgctgca aaaatcggcg ctggtactac ccgtatgacc actaacccgc
     3481 gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactctggc tacttcaaag
     3541 aaggtttctc catgcaaacc ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg
     3601 aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt attaccgcga
     3661 cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgtg cagagctttg
     3721 acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc
     3781 agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg gtggtactga
     3841 gcgcgctgga aattgacagg cagttcaacg ttaacgtgct gaccggttct gacggcgtac
     3901 tgcgtggcgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt tccatcatcg
     3961 tcgcgccact ggtacgcggt cgtattccga ctctggtgga taacgtgctg acctgcatca
     4021 ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt aacccggcac
     4081 gtccggaact agcagaacgt ctgcaggaag cgggcattaa agtggtttcc attgagtggc
     4141 tgcgcgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg
     4201 tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg
     4261 aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc
     4321 tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgc ctggctcaag caccatcctg
     4381 ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac
     4441 gccgaatttt taatcatcgc gtgacagcct tgcgtgcctt agccacaaag cagggctggc
     4501 aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttgagcattg
     4561 ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg
     4621 ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc cgccgcgact
     4681 attcactgcg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4741 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4801 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4861 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
     4921 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
     4981 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
     5041 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     5101 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
     5161 gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca accgtttgtt
     5221 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     5281 gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5341 ccgaagcagg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5401 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggctacca
     5461 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5521 aggcgcaaac attattgcaa aaagggggca ttcgaacccc caccgatctc gattatctcc
     5581 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5641 tgatccttac ctgtttttta gcacagattt aattatttaa gcacttgata aatttggaaa
     5701 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5761 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5821 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5881 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5941 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     6001 atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
     6061 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     6121 catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     6181 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     6241 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6301 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6361 ggaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca gattagctgg
     6421 ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat tgcgccgtgg
     6481 ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc aacctgggcg
     6541 ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct gattggcctt
     6601 gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc tactgcggtt
     6661 ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa agacattacc
     6721 cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt gatggctaac
     6781 ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac gcacttggaa
     6841 ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt tgcacactac
     6901 ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct ggccgtcggt
     6961 aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt
     7021 atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta cggctgtggc
     7081 tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat ttacatctct
     7141 atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt tcgccaaacg
     7201 ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt atgatttaat
     7261 agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc tgccgtaacg
     7321 ccgcgttgct cgcggtttct ctgcttccct tatcttctgc caacgccgta gcgttgcagg
     7381 caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa accggatttt
     7441 gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa
     7501 cgaccaacaa aactgatttt ctgaccgtac atggtctgtg gccaggattg cctaaatcgg
     7561 ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct actcgcccaa
     7621 tcccgaatct accagaagca cgcgccagcc gaatgtgttc atcgccggaa accgggttat
     7681 cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga cgttcctgcc
     7741 tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg gacgcatact
     7801 tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga aaattccttg
     7861 cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt gccaaaagct
     7921 ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct gcgtatttga
     7981 ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct gcaaactcat
     8041 tcttgccaca acctcaccca ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt
     8101 attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa
     8161 ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag
     8221 tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt
     8281 cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca
     8341 gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac
     8401 ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa
     8461 tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc
     8521 gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg
     8581 atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg
     8641 ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg
     8701 aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc
     8761 gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt
     8821 tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac
     8881 catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat
     8941 ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat
     9001 tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa
     9061 tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt
     9121 caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg
     9181 gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt
     9241 tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc
     9301 taacaatgcc gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt
     9361 tccgtggtgc tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg
     9421 tgacctgccg tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc
     9481 gggatcgcgc cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca
     9541 aaaatctgtt ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cacaggacca
     9601 gcaccataga ctgcaacgct tgaaccttgt tggatctgca cattttttgc tgcctgccat
     9661 gccgttggca gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact
     9721 ttaaacggcc ccacattccc tttcgggacg cggacatatt ccgcctgccc gccaggaacg
     9781 ccaccataca ggtgactata accaaacaat gccgcgggag caggtatctg ttttttattg
     9841 agcgcagcgc ctttacccgc attggtattt tcgcaggcgg catattgctg taatcgacag
     9901 aaaaaacagt cgccacaagc aatgacgaac ggaattacca cccggtcgcc tttttgcaaa
     9961 ttttttacgt ccttcccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg
    10021 ccatgtttaa cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc
    10081 gttaccgtaa tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt
    10141 tctacctgaa cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca
    10201 gttcaaaaaa gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc
    10261 aggttttggg agaattccta gtgttcagtg agaatatatt aatcacagaa accgttcgta
    10321 aggaaaaaga accagacaaa tatcagggat aaatattata atcatggaga atgatttgtt
    10381 catgattaac agggagaaag ggttatgtat aaggcaatca ttatgccagt tgatgtattt
    10441 gaaatggaat tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga
    10501 gttattcatc tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc
    10561 gctgatgtgc gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg
    10621 atggtcagcc acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc
    10681 gtgcgggatg aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttatcggt
    10741 tctcgcaacc catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc
    10801 cacgccaatc tgcctgtact ggtcgttcgc taacagataa aagtatttat gccgccctgc
    10861 ctggaacatc ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc
    10921 ggctaagcaa ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta
    10981 gtcaaaagca ctcagagagg ctttggcacc ttcgccagtg gcgatgatga tctgcttgta
    11041 cggaaccgtc gtacagtcac ccgctgcgaa cacgcctttg acgttggttt cacatttcgc
    11101 atcaatgata atctcgccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt
    11161 cggcagcaga ccaatctgga caaaaatacc ggccagttcg atgttgtgaa tattgccgct
    11221 gacacgatct cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt
    11281 ttgcgcattc agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagaacctg
    11341 gtcggctttc atttctggcg caaattccag cagcgttacg tgctcaacga tacccgccag
    11401 gtcgattgcc gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt
    11461 aaacagcggg ccgtcgcagt gcgggcagta ggtcacgcct ttagtgcgat actgatcttc
    11521 gcccggaacg ttcatgttgc gccattttgc acccgtcgcc acgataatgc tgcgtgcttt
    11581 cagtaccgcg ccagaagctg tttcaatctg gtgtagacca ccttcaaccg ctgccgggat
    11641 cagtttgctg gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt
    11701 cagtgcgcct gccaacttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc
    11761 aacggtatcg aggatctgac caccaaaacg ttcgcccatc agaccggtac ggatgccttt
    11821 acgtgcggag taaattgctg ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc
    11881 ataagcatca cgcttgttca gctcttctgc cgcacgattt tccgcgccag tatcaatttt
    11941 ggcaacgatt tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac
    12001 tgccggaacg cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat
    12061 tgcagtgtgc ttgatgcgcg ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc
    12121 cgggcagtta tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg
    12181 gcgaatctgc tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccacagcaa
    12241 cgccagtacc agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggtccctg
    12301 gttggaacct gggttggtga tcaggaaaga cggcttacgc actggcaagc tgttatcttc
    12361 tttaaaggtg actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc
    12421 cgatttagcg ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag
    12481 gtaagccttg agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa
    12541 catcatcatg caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga
    12601 agaaaatcgg gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta tttcgcgtca
    12661 gagcaaggcg actagcctgt gaatccccgg gagcttacac aagtaagtga ccggggtgag
    12721 cgggcgacgc caacgccgct atggcgtgaa agacgaagga aatttagatt ttaccaacca
    12781 ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg caaacttcac
    12841 ctgggtgaga agctacgtac tgagctgctt tgattttacg cagcagatca gacgcgtcac
    12901 ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg tcaacaacga
    12961 aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg
    13021 cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca gagctgctgt
    13081 gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct acgcccagtt
    13141 tctgcagttc ttcgtagtgg tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa
    13201 agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt tcggtgattt
    13261 cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg gtgttaatca
    13321 aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat
    13381 tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct tcgtaagaca
    13441 actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc tttccaggtg
    13501 accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg
    13561 cccttacaaa tgcaacaacg acatggatta caacaccctc ataaacaaag ggcaatcacc
    13621 tgatctaagc tcttacctat gacagtgata ggttatgcct tttactcgac ttttgcactg
    13681 actgaaaaga acaaattaat gttaaaaaag atacttttac tggctctgct tcctgcaatc
    13741 gccttcgcag aggaacttcc ttccccagta aaagcgattg aaaaacaggg cattacaatc
    13801 atcaaaacgt tcgatgcccc cggaggaatg aaaggttatc tcggaaagta tcaggatatg
    13861 ggcgtcacca tctacctgac tcccgatggt aagcacgcta tctctggtta catgtacaac
    13921 gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc accagccgga
    13981 cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg
    14041 ccggtcattg tctacgtctt ctccgatccg ttctgcccat attgtaaaca gttctggcag
    14101 caggcgcgcc cgtgggtaga ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt
    14161 atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa
    14221 acctggcaac aatatgaagc ctctggttgc aagcttaagc taaacgtgcc tgcaaacgta
    14281 agtacagagc aaatgaaagt gttaagtgac aatgagaaac tgatggacga tctgggggca
    14341 aatgtcacgc cggctatcta ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg
    14401 ttgcccgatc agaaaacgct taatatcatt atgggaaata aataaaaggt aattcatgga
    14461 tggttgaact atatccctgc ttttcaacca tcctaaaaaa gcatgaattt ctcgctctac
    14521 cctaagtggc tgtgctattt tagaaaaact taatcctgag ttaagcaaaa aaacataatc
    14581 aataaaaata catacgcaaa aaacattgat taagtgaata taccatggaa gaaaaatata
    14641 aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta aatcttcttg
    14701 tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa tcactatata
    14761 tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt aatgaccctt
    14821 tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc
    14881 atcttgagaa aaacctcaaa ggccttgaac aaacaattaa tatagtcaat aagtccgaac
    14941 taaagaaaaa ctttattatc tacggccctc agctcatttc ttgctctaat aatagtatgc
    15001 tgatccgctg cctacggcaa gatgcctctg tcgagattga gtgccatgat atcctcatgt
    15061 cagcagagaa tgctgaagaa ctgttggttc accgcaaagc cgatctggtg atcacccaaa
    15121 tgccagtgat cagccgttcc gtcatttgca tgccattgca tacaattcgc aatactctaa
    15181 tttgtagtaa taagcatccg cgaattactg acaatagcac ttatgaacaa attatggcag
    15241 aagagttcac ccaattaata gccaaatcag caggagttga tgatattcaa atggaaatag
    15301 acgagaagtt tatgaatcgc aaaatttcat ttcgtggttc ctctttgctg acaataatta
    15361 atagcattgc agttacagat ttactgggaa tagtccccta tgaactttat aactaccacc
    15421 gtgactttct taatttaaaa gagataaaac tagaacatcc gttaccttca ataaagcttt
    15481 acatttccta taataaatct tctttaaata atttggtgtt ctcccgattt attgaccgtc
    15541 taaatgagag tttttgatat ataactatag acagctacta taactttcat ctatttattc
    15601 acagcgcgaa tgaaaaataa aacactctgc tttacagtgt ttactttata attaacagtc
    15661 ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat
    15721 tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctaga cactcaacac
    15781 cctaccccgc gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct
    15841 gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcaactggga
    15901 ggc
//
LOCUS       DAZPDE010000091        15759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPDE010000091 DAZPDE010000000
VERSION     DAZPDE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.091"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..611
                     /locus_tag="ACHU0J_002547"
     CDS             <1..611
                     /locus_tag="ACHU0J_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR1217196.1"
                     /translation="DFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFD
                     SVINQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRI
                     TIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYE
                     KLNSKSSFYLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(680..1636)
                     /gene="yjiA"
                     /locus_tag="ACHU0J_002548"
     CDS             complement(680..1636)
                     /gene="yjiA"
                     /locus_tag="ACHU0J_002548"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1217197.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1647..1850)
                     /locus_tag="ACHU0J_002549"
     CDS             complement(1647..1850)
                     /locus_tag="ACHU0J_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1217198.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(1900..4050)
                     /gene="btsT"
                     /locus_tag="ACHU0J_002550"
     CDS             complement(1900..4050)
                     /gene="btsT"
                     /locus_tag="ACHU0J_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1217199.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4443..4955)
                     /locus_tag="ACHU0J_002551"
     CDS             complement(4443..4955)
                     /locus_tag="ACHU0J_002551"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1217200.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(4973..6531)
                     /gene="hpaB"
                     /locus_tag="ACHU0J_002552"
                     /pseudo
     CDS             complement(4973..6531)
                     /gene="hpaB"
                     /locus_tag="ACHU0J_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6758..7695)
                     /locus_tag="ACHU0J_002553"
                     /pseudo
     CDS             complement(6758..7695)
                     /locus_tag="ACHU0J_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7722..8612)
                     /gene="hpaA"
                     /locus_tag="ACHU0J_002554"
     CDS             complement(7722..8612)
                     /gene="hpaA"
                     /locus_tag="ACHU0J_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1217201.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8622..9977)
                     /gene="hpaX"
                     /locus_tag="ACHU0J_002555"
     CDS             complement(8622..9977)
                     /gene="hpaX"
                     /locus_tag="ACHU0J_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1217202.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(9999..10802)
                     /gene="hpaI"
                     /locus_tag="ACHU0J_002556"
     CDS             complement(9999..10802)
                     /gene="hpaI"
                     /locus_tag="ACHU0J_002556"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1217203.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10813..11616)
                     /gene="hpaH"
                     /locus_tag="ACHU0J_002557"
     CDS             complement(10813..11616)
                     /gene="hpaH"
                     /locus_tag="ACHU0J_002557"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1217204.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11684..12064)
                     /locus_tag="ACHU0J_002558"
     CDS             complement(11684..12064)
                     /locus_tag="ACHU0J_002558"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1217205.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12074..12925)
                     /gene="hpaD"
                     /locus_tag="ACHU0J_002559"
     CDS             complement(12074..12925)
                     /gene="hpaD"
                     /locus_tag="ACHU0J_002559"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1217206.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(12927..14393)
                     /gene="hpaE"
                     /locus_tag="ACHU0J_002560"
     CDS             complement(12927..14393)
                     /gene="hpaE"
                     /locus_tag="ACHU0J_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1217207.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14390..15679)
                     /gene="hpaG"
                     /locus_tag="ACHU0J_002561"
     CDS             complement(14390..15679)
                     /gene="hpaG"
                     /locus_tag="ACHU0J_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1217208.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3697 a         4128 c         4132 g         3802 t
ORIGIN      
        1 atgatttttc tcaatttatt accgcctttg agaaggtata tatgcatggg tggttaaaaa
       61 aacaaattaa aagccaaaga gaaatggttt gttattctgc tctggtagca ataaataatg
      121 atatgccttt tgattctgtg ataaatcaga taaaccaaca tgcagataat tcaggtttta
      181 tagctgctct ggatgaagat ttatatgagc ctcgtccgaa tcaagtgaac ttaataaaag
      241 caattttgct tcgcttagat atggagcaac aagatgagag cgtgattaaa acctatacgg
      301 ggagaatcac aattgagcat attcttcctc aagcattagt taatgaatac tggattaata
      361 gattccaacc tcaagagcat gtctactggc tgcataaaat aggtaattta acactcataa
      421 gtggttccaa aaattctgaa acacaacact atgattttat caaaaagaaa tctatttatg
      481 agaaacttaa tagtaaaagc tctttttatt taacaaaaga tgtttgtaat tcttcagaat
      541 ggggattggc tgaactgaaa atgagacatg aaaaaatgaa aacgcaattg aagaagcttt
      601 ggttggtctg acatatctaa agagttccac tcgtaataat gttgcggtgg aactctcaaa
      661 aagttgatct ttcccgaact tacttcctca atcccgcaaa cgccgcccga atctcttcct
      721 ccggcaactg aatcccgata aacaccattg tgctatgcgg tttttcatcg ccccacggcc
      781 tgtcccagtc ggcgctgtag aggcgctgga cgccctggaa cagcaggcgg ttaggttcgc
      841 cgtcaatcca cagcatccct ttgtaacgca gcagtttatc cgccgattcc agcagcaggt
      901 tttccatcac gcgggaaact tcgctgatat ctaccgggta atccagttcc accacaattg
      961 acgaaatatc gttttgttta tccgcgataa agtggaaacg cggtttggtg ctgacgacgt
     1021 tctcttccag cataaaaccg ttggtgttga acagcaggcc caggtcgatg tcgccgtggg
     1081 tgacggtgta gaccggtgcg cgggcgttga tgcgcgccag gcgttcacgc agtttctctg
     1141 cttcgcctgc gacgtcggtt ttagtcagca gaatacggtc ggcgtagccc acctgggact
     1201 gggcgatggt gaactggttc atctgctcat cggcatgtac cgcatccacc agcgcaatca
     1261 cgccgtccag caggtagagc tggcataaaa tctcatggga gaaaaaggtc tgaataatcg
     1321 ggccgggatc ggccatgccg gtgcattcaa tcaccagacg atcgaactga atattgccct
     1381 tgtcgagatt atccagcagg tcgagtagtg cgtcctccag ctcgttggag cgcgaacagc
     1441 agatgcagcc gttggtcagc gttttgatct gcgtggcgcg gtcgccaatc agttggtcat
     1501 caacggagac ttcgccgaat tcgttttcaa tcacggcaat cttgtagccg tgttgttcgt
     1561 taagaatatg gcgcagcagg gtagttttgc ccgcgcctaa aaaaccggtg agtagggtaa
     1621 ctgcaatcgg gttcatgctc tctccattaa caacagcgca taccgccttt accatcgccg
     1681 ccgtagcgcg cgttctggcg ttcgcggaag aattcttcat agctcatgta cggcttgtcg
     1741 ggatggttgg tcttcatatg ctcgacgtag ttgtcgtagt ctggaatacc aatcaacatc
     1801 ttcgccgcct ggccgagata tttttttgcc tgtcctaagt taccaaacat agttcactct
     1861 gataagaaca aagccccgcc gaagcggggc taaacacggt tagtggtgcg aagagatctt
     1921 cacgccgcct tccgggattg gaacgtacgg tgtttctttg tcagtacgtt tgtcgctgtt
     1981 acgcaccgca agccaggttt tgaaaccgta gaagatgatg ctgtacacca caatcaggaa
     2041 cagaatactc aggcctgcgt tggtgtagtt gttcacaacg atgtggttca tgttggcaat
     2101 ctgctgcgcc gtcaggtcag taccgttagc aatcttctct ttgtactggc ttgccatgta
     2161 gaagaagcct tccatctgcg ggttggtgct gaacagtttc agacccagcg cccaggtggt
     2221 gcagataagc agccatacag ccggaacaac agttacccag atgtattggg tgcgcttcat
     2281 cttaatcagc acaacggtgc ccagtaccag cgctacggct gccagcatct ggttggagat
     2341 accgaacagc ggccacaggc tcttaacgcc gcccagcgga tcgaccacgc cctgatacag
     2401 caggtagccc cacagaccca cacagcccgc agtaccgatg ataccggcaa ccagagagtc
     2461 ggtttttttc aggaacggga tgaagttacc cagcaggtct tgcagcataa agcggccaga
     2521 acgggtaccc gcatccagcg cggtcaggat gaacagggct tcgaacagaa taccgaagtg
     2581 ataccagaag cccatgtcag ccatcggcag cactttgtgg aacacgtgag cgatacctac
     2641 cgccagcgtc ggcgcaccac ctgcacggtt caggacagaa ggctcaccaa tgtctttcgc
     2701 ggtttgcagg atctgctctg gcgaaatcac gaagccccag gagctgacgg tcgctgccgc
     2761 gtgtgcggta acgtctttca gctgcgccat gatgatcggc gcgttctcgc cacccatttc
     2821 atgcaggtta ggcatggtga tgccaaggcc agcaggcggg gtgttcatcg cgaagtaaag
     2881 acccggttcg atgatggacg ctgcaaccag cgccataatc gccacgaagg actccatcag
     2941 cattgcgccg tagccaatga aacgcgcgtc ggtttcgtta gccagcagtt tcggcgtcgt
     3001 accggaagag atcagcgcgt ggaagccaga taccgcacca caggcgatgg tgatgaacag
     3061 gaacgggaac agagcgcctt tccacagcgg gccagtaccg tcaatgtact gggtcatcgc
     3121 aggcattttc agttccgggt tcagcaccac gatacccagc gccaggccga cgataacgcc
     3181 gattttcagg aaggttgcca gatagtcgcg cggtgcgagg atcagccaca ccggcagcag
     3241 tgcggaaaca aacgcatagc caatcagcgc gaaggtaatg gtggtgtctt taaaggtcag
     3301 tgccggaccc cagtacggat cgtgagcaat cacgccaccg aagtagatag aggcaaccag
     3361 cagcacgata ccaataacag agacttcacc cacacgcccc ggacggataa agcgcatgta
     3421 gatacccata aacagcgcaa tcggtacggt tgagcaaacg gtgaagacac cccacggact
     3481 ttcggccagg gctttaacca caatcagcgc cagaacggcg aggatgatga tcatgattaa
     3541 gaaacagcca aacagcgcga tagtccccgg tactggcccc atctcttctt tgatcatctc
     3601 accgagagat gcgccgttac ggcgagagga gataaacagc accataaagt cctgtaccgc
     3661 accggccagc actacccccg ccagcagcca cagcgtgcca ggcaggtagc ccatctgcgc
     3721 ggcgagaacc ggtcccacca gcggaccagc accggcgata gcggcgaagt ggtgaccaaa
     3781 caacacgtaa cggttggtcg gaacgtagtt cagaccgtcg ttgttaataa ccgcaggcgt
     3841 cgcgcgcgtg gggtcgagtt tcatcacctt ctgggcgatg tacagactgt agtagcgata
     3901 cgccaccaga tacaccgaca cagaggcgac cacgatccac agggcgctga cgtgctcccc
     3961 ccgacgtaat gcaactaccg cgaggcagaa tgcaccgatg attccgagaa tcacccaggg
     4021 tatgtgcttg aatagttttt tcgtatccat agtaaaacct ggcattttac tcgtcgtctt
     4081 ttggcttaca ggtgcggcag ctgcgttcgt tcacctctga cacttacttt tgtaagctcc
     4141 cggggattca ctcacttgct gccttcctgc aaaccaaaat ccatagagta atttgttaat
     4201 cataggccga agccgttcta ggtttgcgtt gcgtttgagg aggtaaattg accgctatcc
     4261 tgcagtgatg ttgccagaga tacgtgcgcg taaagtaagg taaataactg agtggttatt
     4321 ttagggatgt aagcggtcag gaatggcggt gagcggttgg gcaagaatta ttcatcgggt
     4381 agatatgtga ttgagatcac aataaaaatg ccccttcgac gaatgcgtga aggggctgga
     4441 gcttaaatcg cagcttccat ttccagcatc accggatgga aacggcgttt aaagtaaata
     4501 agtccgtgac cttctgcact gaggatgatg tttttaatct ccaccagata caccagatgt
     4561 gtgccaatcg cctgcacatc gcggatctct ccttcaagac tggccagcga accttttagc
     4621 accggctgcg ccagcggacc tttttgccag catgagaggc taaaacgctc ttccatcgcc
     4681 atgcctgtca tgcccgcgaa gtggcgcgcc atcagttcct gctcatggtt gaggacgttg
     4741 acgcacaact tgccgttgcc ctgaaaaacc gggttcatcg cactgttggc gttaatgcac
     4801 accatcagcg acggtggtgt atccgtgacc gagcagacag ccgttgccgt aatcccgcat
     4861 tgtccggcgt cgccctcggt ggtgataata tttaccgctg ccgacaggct ggccatcgcg
     4921 tcatgaaagc gcaggcgttg ttcatctaat tgcatcttaa cctcctgctg cgttatttca
     4981 gcagcttatc cagcatgttg atatcgtcgt tgttgtgcag gtgcggcaca gtccagccgt
     5041 tctggtcgta ttccgacagg cagcgatcaa ccatcgccat catcttgtcc atattgccgg
     5101 agctttgtgc ctggcgcaga cattgcaggc gaatctcatc ctggctaccg gagtagttga
     5161 tttcatacag ctcgtgacga ccaccaaatt cgctgccaat ggcgtcccac atcagtttga
     5221 ggatcttgat gcgctggacg tgatccatac cgttcgaacc gcgcacatac ttcgccagat
     5281 actggtcgat ctgcggattg ttcaggtcac gggcactgga agggaggtag atcaggccac
     5341 tggtaacgtt gcgttctata atgtttttga tcttcgcgta ggccattggt gccagtacgc
     5401 gataggtttg cagtgcggca tgatccggta aataagcccc gttgacccac ggcgtcgctt
     5461 ctgaacacat cgagtcactc aatgcccaga aggtgttgcg ccacgccacc acttcaccga
     5521 gatcggcctg cacaccacgg aactccaggg tgccggtaca ttcgagtgat tttttcagca
     5581 gtgccgtaat gaagtcgagt ttcactgcca ggcgcacaca ggcttgcagc ggatacatac
     5641 gggcgaaacc gccttccatc gtccagcgac ggcagcgatc aaaatcgcgg tagatcagca
     5701 cgttttccca tgggatcagt acgttatcca tcaccagaat cgcatcgttc tcatcgaagc
     5761 ggctggagag cgggtagtcg tatggcgagc cggtagcacc cgcgaccatc tcataagagg
     5821 cgcgggagat taatttcacg ccatcggcat ccattggcgc aacgaacatc agtgcgaagt
     5881 ccgggttttc gcccatcact tgtgccgagc cgaagccaat catgttgtag tgagtcagcg
     5941 ccgagttggt ggcaaccact ttcgcaccgc tgacgataat cccggcgtca gtctcttttt
     6001 ccagcttgat gtaaacgtct ttcactttat cggtcggcaa atgacgatcg gtgggttaac
     6061 aatcgcgtgg ttaaagtaga ggccagtttc ctgaatacgg gtataccagt tacgggcgtt
     6121 ctgctcgaac tgaccgtaaa agcccggatt cgcgcccagt gcgcaaccga aagcggcttt
     6181 gtagtctggg gtacggccca tccagccata gctcaggcgt gaccattcag cgatggcgtc
     6241 gcgttgctgg cgcaggtcgt cggcactttt cgccacgcgg aagaatttat gggtatagcc
     6301 gccgctgccg gtgtcggtgt tccaacacag agagtcctgc atctccggtt tgtgcagcgc
     6361 gtcgtacagt tgggcaacag acgctgccgc attacgaaat gccggatgag tggtgacgtc
     6421 tttcactcgc tcgccataga tatagatctc gcgaccatcc tgcaggcttt tcagatactc
     6481 ttccccggtg aaaggacgtt gggtactggc gcggaaatct tctggtttca tgtcgacctc
     6541 tacagtatta atcggaatac gattgttaat tttgtgtgtt gtttttgtta tttaattgaa
     6601 gcgttgaacg cggttgtcgg gaagagacgt ttcaggcgaa cgttggtact tttcgacagc
     6661 aaggtggagt taaatagtaa actgcgatcg tgatcaactt acagtaaggc aaatcataat
     6721 attccccgct aacaagggct aacggggacg gaatttttta tggggcgaat ggaatggtaa
     6781 tcgtcatatt ttcatgacca acgacgatag gacctgaata gttttctgtg cttcttgctt
     6841 ccatgtttca tccggaacgg tgattggtac caggtggcta aggatgagtt ttttcacttt
     6901 tgcttcattg gcaattttgc ctacatcctc aatagtcgta tggtgagaag caatggcttt
     6961 tgccagtgtt ttaccattgc caatactgtc tgccagctta gcgacggagg gctcatgtac
     7021 ggcctcatgc acaagtatat cagcgccttc tgagaaagtt gccagtgggg gaaagtacga
     7081 ggtatctcct gagaagacaa ttcgcttgcc ttgtgtgtcg aagcgataag caaaagcctc
     7141 accgtcaggg aaaggagaat gagggacctt taatgcagtc acggtcacgg tatcatcttt
     7201 ataaactacg ccttcaccaa catcttgagc tgtaacgagt ttgcgaggat cgggttttcc
     7261 ttcttcttcc atacgcagtg tgatgtcccg atcgaagacc tgccaactgg cagcggtcat
     7321 ggctcgggtg cctggcggac cgaatacatg aatagttttg tcctgcagac cgctagccca
     7381 tgcattcatt aagactgatg gatagtccag aatatgatca ctgtgtaaat gggtgagtaa
     7441 aatattatca atctggcgca gtggaatatt attttttacc agttgtaagc tggccccgta
     7501 accagcatca attaaccagg ttttattgcc aataataagt gccgttgcca tcggtaaacg
     7561 gtcagctttt aataaagaag ggccaccttt cgttccaagt agtacaattt tcatttattg
     7621 cggttcggag gcgtaggtga atgccactgg cgcaatcagg cacgccacaa atagtgagcg
     7681 taaagatatt ttcatgtcga gtctctcagg ttgataaatg gtcagcgacc acggaattgt
     7741 gttggtgagc aacccatcat tcggttgaaa aaacgggcga aataggcggg atctttgtag
     7801 ccaagatcaa gggctatctg gttgatgctt tgctcgctat agagcagaag ccgtctggct
     7861 tcccggctca ctcgttcgaa aatcaatctc ttcggcgatt gattggcaaa acgtcggcac
     7921 agctcggtaa gccgtgattc actcataccg agcattgttg catattcagg gaccattaaa
     7981 tgttggcgat aattttcgtc aatcagccga ttaaacttct ggaataaaca catctcgccg
     8041 cgcacgctgc atgttgaaat atcatttggt ggtgcttcac gcagtaggat tgtaaaaata
     8101 ctttgcgcca gtgattgcaa tagaatttcc cgtccttgct tattttgttg aaactccagg
     8161 gatattatcg gccaataatg atttaatgcc gccagtgtat gttccattcc ctccagtgac
     8221 agacagatac cttgtagcat tgcggcatct ccactaccag gccagagctt ttctaccagg
     8281 ggccaaatta actcctgacg tacggtcaga acatgcccat cgctatcagg atcagtaaag
     8341 aaagtatgtg gcaccgatgg tggtgttagt acaaaaagcg gtgcgcgcac agaatagaag
     8401 tgctcatcca gttgtaaagt aatgcgtcca gtgaccagat aatggagttg aaaatatcta
     8461 tcgtgccagt gagggcgcat atcgcgtcca aagaaattcg ctaaccgggc aaatgtttca
     8521 taatgcacat cttcaccagc gtagcgggca tcatattccc ggaccaaatc aatattttca
     8581 taaccatgtt caaagatagc ttcactggac atcatgactc cttattgcgg ttttggtgcg
     8641 ataagttcag atgaaagaga gtgttcccga ctccccatag ggattcgggt catgactaac
     8701 gcgcccagta ccagcaatcc tgctacaaac cacaaacctg agctaaaact gccggttgca
     8761 tctttcaaaa taccgataag taatggactg actgctgaac ctacattgcc gacagcattg
     8821 atgacagcca tcgcaacggc acgcgattgc aagcttataa cttgatccgg tgttgtccaa
     8881 aagatagtca tcgcggtaaa tgatcccatt gaggccataa tgatgctgct cagttgaatg
     8941 aggctgtgat ctgtcgcaga agcgagtaac catccggcag cggcaaacag gtagggaaga
     9001 atcgtatgct ttttgcgttc tttaagacgg tcggaacgac gactccacca gatcatgcca
     9061 aaaattgtgc agaattgggg gatggccgcc agcaaaccaa tcatgatgtt gctactgcca
     9121 gtattgaaac tctgtaggat ttgcggcgtc cagatattaa tcgcactcag agtattagtc
     9181 gggcaaaaat aagcaagtgt atatagcatg atggctgggg tcaggacttc acgtaaggtt
     9241 gagcgtggaa cagatgcatg ggcaaatgca ctctcttgtt cacggagaat catcgttttt
     9301 aaagcctgtt tttcgttttc atcaagccag gtcgcctggt ctggtgtgtc gttgagataa
     9361 aaccaggtga cgacaccaag aatgaccgat ggcagacctt ccagcaaaaa gagccattgc
     9421 cagcctttta gattccacag tccatccaag gccagaatgt agcctgaaag gatcaatccc
     9481 agcatcatag tgactggcat cgctatcata aataaggcat tggctctggc ccgatgatat
     9541 gccgggaacc accaggtcag ataaaccaaa ataccaggta aaaaacccgc ctcagcaata
     9601 ccaaccagca tacgcagtag atagagtgtt tcggggcttg ttgcaaacat tgtgcaggtg
     9661 gaagcaattc cccagaccac catgatccca gcgatccatc gacgagcgcc aattttcgct
     9721 aacatcatat tactggggat cccgcacatg acgtacgtga cataaaagag tgttgcggcg
     9781 aggccaaaca ttgtggacgt caggccaaga tccttgccca ttgtcagtcc ggcaaatcct
     9841 atattgatgc gatcaagaaa cgaaaaaaca aacagaataa aaagaaaaat gattaggcga
     9901 cgaaataatt tattaatgac acgatgttca atttcaacag ttttattatc tttatattgc
     9961 agggtagagg tcgtcatgat gcgctccaca tcgtacgttt agtagacaga gttgttgttt
    10021 tgcttcgcca tcgaaataga agaactatta ccaaaccgtg ctgccagcgc ttcggcggcg
    10081 cgggcgagca gggtggtgtc aacgccgacg gcgacaaaca gcgcgcccag ttccagatag
    10141 cgttttgcca gttgctcatt ggcgatcagg atccccggcg ctttgcccgc ttcgcggatc
    10201 tgcacgatcg cctgctcaat ggcggcctgt acttccgggt gctgcggatt accggcataa
    10261 cccatatcgg cgctcagatc cgccgggccg ataaacacgc cgtcgacccc ttccacgtcc
    10321 agaatctgcg gtaagttctt cattgcctca cgcgtttcga tctgcaccag cacgcacatt
    10381 tgatcgttgg ctttttgcag gtaatcagga atgcgattcc agcgcgaggc gcgagccagc
    10441 gcactgccca caccgcgaat accggcgggg ggataacggg tggcgcgtac cgctttacgg
    10501 gcttcgtcgg cgttttgtac catcggcacc agtaaggttt gtgtgcctac gtccagcagt
    10561 tgtttgattt gcaccggatc gttccacgat gggcgtacca ctggctggct ggggtagggc
    10621 gcaatcgcct gtagttgggt gagcacggtt tgcacgttat tcggcgcgtg ctcaccgtcg
    10681 atcaataacc agtcgaatcc tggtccggcc agtaactccg cgctgtagct gctgctcagc
    10741 cccagccata atccaatctg cggacggcct gctttcagcg ccgctttaaa actgttttcc
    10801 atcgttctct ccttaaacaa agcggcagct aatggagccc atgttgccgt aatcgacgtg
    10861 gaaggtgtcg cccttacgcg ccggaaccgg gagggtgaac gaaccaccga gaatgatttg
    10921 cccggtttcc agttgtacgt catacggtgc gagtttgttc gccagccagg ccacgccgtt
    10981 tgccggatga ttcagcacac cagcggcgac gccggtttct tcaatcacgc cattgcgata
    11041 catcagggcg gagatccaac gcagatccag ttcgtctggt ttgatcggac gacctccgag
    11101 gatcactccg gcgttggcgg cgttatcgga aatggtgtcg aatactttgc gcgggcgctg
    11161 ggtttccgga tcgatgttgt ggcagcgagc gtcgatcagc tccagcgccg ggatcacata
    11221 gtccgtggcg ttgtaaacgt cgaacagcgt gcagtttggc ccacgcagcg gttttgccag
    11281 cacaaaagcc agctccactt caatgcgcgg cacgataaag cgatcggtcg ggatatcgct
    11341 gccatcgtgg aagaacatgt cgtccagcag tgcaccgtaa tccggttcgc tgatctgcga
    11401 gcttgcctgc atcgctttcg aggtcagacc gattttgtgg cctttcagcg tgcgactttc
    11461 ggcgattttc aggcgaaccc attcgcgctg cacggcgtag gcgtcttcga tggtgatctc
    11521 cgggtaatcc agcgagatcg cgcggatctg ttcgcgctgt ttttctgcct gatccagacg
    11581 ctgggcgatc agggtgtggg tgtgtttatc gaacatagcg atatcctgtg gcattgccgg
    11641 atgcggcagc gccttatcat ccggcctgga agctggtgca aatttattta aacaacgcgt
    11701 gtacgttgtt ctgtttgaaa ttcaacgtcg ggtgcagctc tgcaatctca aatgaaagcg
    11761 ctaggtaacg gctctccatc agtgaggcga agtgcgtttt aatcagatca aacagcattt
    11821 cgcccacttc ctgacggctt tccagactgc gtccgctgcc gatttttagc gtcatatgca
    11881 caaaggcgta atcgtgcttg ccatcagcca tttgccaggt atcgatccag tgcgcccggc
    11941 tacgtattcc cccaagcgga aaaatccccg ttgccgccag tgcagtgtta acactggcaa
    12001 acagctcagg taagcgggct tcttcacgaa tattttcagt acattccacg ataaagtgcg
    12061 gcataaaacg tccttacgcg ggcagcggga aaacagcgtt aacctgaccg gtgccagagc
    12121 ttgggaacag ctcggtaata aactccacct tgccgtcgta tttatcccag ccgagcatcc
    12181 ccagcagcat caccgtgtcg tgcatattgc cttcgccgta gcagtagtcg gcgtactccg
    12241 gcagcatatt gcaaaactct ttgaactggc cttcgcgcca cagcttcacc acacgctcgt
    12301 ccatctggcg gtcgaactcg cgggtgtagc tgttcatccc ttcttccgca cgctgatcgt
    12361 caataaagcg gtgcgataac gaaccgctgg caaggaccgc cacggtgccg tcgtactgtt
    12421 cgatcgcttt cagaatcgct tcgcccagct tgcggctgtc ggcaaaatcg tgaaccgtgc
    12481 agaaagccga aatggagacc actttgaagt gcttgtcttc attcatgtag cgcatcggta
    12541 ccagcgtgcc gtattccagt ttcaggctgg gaatattgtg cgcttttgcc cgcacgccga
    12601 gctttaaggc ttcctcggca attagctgcc ccaactccgg gttgccctcg tagttgtagg
    12661 tcatgtcgcg aataaaatgc ggcagctcgt tgctggtgta gacgccttca aaatggtctg
    12721 cacagttgat gtgataagca ctgttgacca gccagtgagt atcgaaaacg ataatggtat
    12781 cgacgcccat ttcccggcaa cgcttgctga tctctttatg cccgtcgatc gcaccctggc
    12841 gacaaccgtg gttttttcct ggtagttcag agagatacat cgacggtacg tgcgtgatct
    12901 tggcggctaa cgctaactta cccatatcag actccccatt tcggaattgg atggtcgccc
    12961 atggaaatgc agacgttctt catttccgcg aacacttcga agctgtactc accgccttca
    13021 cgcccggtgc cggaggcttt tacgccgcca aatggctggc gcaggtcgcg cacgttctgg
    13081 gtgttgacga acaccatgcc tgcttcaatg ccacgcgcca ggcgtaacac tttgctgaca
    13141 tcctgtgtcc agatgtacga cgcgaggccg tactccacgt cgtttgccag gcgtaagcct
    13201 tcggcttcgt ctttaaacgg cagcaggcag gcgaccggcc cgaaaatctc ttcctgggca
    13261 acgcgcatac ggttatctac gtccgccagc accgttgggc gcaggaagtt gccgcctttc
    13321 aggtgcgcag gcaggtcgga cggtttatcc gggccgcccg ccaacagggt tgcgccttct
    13381 tcaatgccga gacggatata gccggagact ttttcccagt gttgctgact gataagcgcc
    13441 ctaacctggg tattcggatc gttcggatcg cccacgcgca gacggttggc gcgttcggca
    13501 aagcgtttaa cgaattctgg atagatgctc tgctgaataa agatgcgcga accggcggtg
    13561 cagcgttcgc cgttgattga gaagatggtg aacagggcgg cgtccagcgc acgctcaata
    13621 tcggcatctt caaaaatcag caccggtgat ttaccgccca gctccatgga gtattttttc
    13681 agcccggcgt ttttcatgat gttgcgcccg gtggcggtac cgccggtgaa cgacacggca
    13741 cgcacgtcat gatgacggac cagcgcatcg cctgcggttg cgccgtagcc ctgtaccacg
    13801 tttagcacgc ccgccggaat accagcttcc agcgccagct cacccaggcg gtcggcggtc
    13861 agcggggaga gttccgacat cttcagcacc gcggtattgc ccagcgccag acacggcgcg
    13921 accttccagg tggcggtcat aaacggcacg ttccacggcg acgccagcgc acaaacgcct
    13981 accggttgca ccagcgtgta gttgagcatc ttgtcgtcaa ccggataggt cttgccgttc
    14041 atctgctggc agacttccgc gaaaaattca aagttgtgag aagcgcgtgg gatcaacaca
    14101 tttttggtct gatggatcgg caggcccgtg tccgcggttt ccatcgcggc gatctcaggc
    14161 acgttctggt cgatcagatc gcccagacgg cgcatcaggc gcgcacgctc tttcatcggc
    14221 agatcagccc atttcgggaa cgcctctttc gctgccgcta ccgcctgatt gatctccgct
    14281 tcaccgccag aggccacatc cgccagcact tcacccgttg ccggattggt agtctggaag
    14341 tagtcgttac ctgcaacatt tttgccgttg atccaatgat ttactttttt catttcgctg
    14401 tttcctcact cacaattcgg ttcaccaggc ggcccacgcc ttctacttcc accactactt
    14461 catcgccagg caccacgtca gataagcctt ttggtgtgcc ggtggcgatc atgtcgcccg
    14521 gattcagggt cataaattcg cttaagtagg cgatcaggaa gggcacgctg aagatcagat
    14581 cggcggtggt gccttgctgg cgtaactcgc cgttgacgaa ggtgcgaagg gtcagattat
    14641 gcgggtccgg gatcgcctct ttcggcacga tggttgaaag catcggcgtc agtccgtcgc
    14701 ggcttttgac ccgcaggtta gggcggtagt agttttccag atagtcgcga atggcgtagt
    14761 cgttacacac ggtgtagccc gcgacataat ccatggcatc ggcttcgctg acgttacgcg
    14821 cctgcttgcc aataactacc accagctccg cttcgtagtg catgtattca atattgttcg
    14881 ggcgcacgga ggtctggttg tcgccagtga gggtattcgg cgctttcagg aacaccagcg
    14941 gctcttccgg tggcttaaat tccagttcgc tggcgtggtc ggcgtagttc aggccgaggg
    15001 caaacagcgt accgtgcggg tgtggcagcg ttgggaagct cttgcgcgtg gtcacttcac
    15061 gttcgtccac taccggattt tccagcggcg ggaaaccttc tgcgagaaca cgcacgcgat
    15121 cgcttggctg tatttccacg cgcgcctgtg gcgtgccgag cagaatagca tcgcctggat
    15181 tcagcgtggc aaattcgctc agggcgctca gcagctgtgc ggcgttacgt tgtaaatcgg
    15241 cggtattcca gtgatcggca ggacgcccgt tgatctcggt atagatagtc agattatcga
    15301 cattgctgag agccacggtt tcgccaatgg ggcagaatcc atcacgacat tttgctttga
    15361 ttgccggacg gtaaaagctc tcttccggca ggctgacgtc gttatccagc gcatatccgg
    15421 cgatgtactc tgccgcatct tcttcacgta ctttcgtcgc cgttttcccc acaatcagcg
    15481 caacagtcgc accgctcagt actttttcac cctgtggaaa gggaatcggt tcaccgcaac
    15541 caatcaccgt attgcgcggt ttaataaacc agaccgcagt tttaggcggg gctttgtagg
    15601 gagattgctg gaacgcttcc tgccaggcat caagttggct gcgatggttc aacgctacgg
    15661 cgaagatagt gcctttcatt accactcctc ggattcgatc atcttaatca ttaatatgtt
    15721 aatgattact tttattcccg attgcgtagt ttggcaaaa
//
LOCUS       DAZPDE010000092        15460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPDE010000092 DAZPDE010000000
VERSION     DAZPDE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.092"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(116..868)
                     /gene="tcyN"
                     /locus_tag="ACHU0J_002562"
     CDS             complement(116..868)
                     /gene="tcyN"
                     /locus_tag="ACHU0J_002562"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1217209.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="ACHU0J_002563"
     CDS             complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="ACHU0J_002563"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1217210.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="ACHU0J_002564"
     CDS             complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="ACHU0J_002564"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1217211.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2639..3439)
                     /gene="tcyJ"
                     /locus_tag="ACHU0J_002565"
     CDS             complement(2639..3439)
                     /gene="tcyJ"
                     /locus_tag="ACHU0J_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1217212.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3527..4078)
                     /gene="fliZ"
                     /locus_tag="ACHU0J_002566"
     CDS             complement(3527..4078)
                     /gene="fliZ"
                     /locus_tag="ACHU0J_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1217213.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4124..4843)
                     /gene="fliA"
                     /locus_tag="ACHU0J_002567"
                     /pseudo
     CDS             complement(4124..4843)
                     /gene="fliA"
                     /locus_tag="ACHU0J_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5008..6660)
                     /gene="fliC"
                     /locus_tag="ACHU0J_002568"
     CDS             complement(5008..6660)
                     /gene="fliC"
                     /locus_tag="ACHU0J_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR1217214.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6926..8332
                     /gene="fliD"
                     /locus_tag="ACHU0J_002569"
     CDS             6926..8332
                     /gene="fliD"
                     /locus_tag="ACHU0J_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1217215.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8357..8767
                     /gene="fliS"
                     /locus_tag="ACHU0J_002570"
     CDS             8357..8767
                     /gene="fliS"
                     /locus_tag="ACHU0J_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1217216.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            8767..9132
                     /gene="fliT"
                     /locus_tag="ACHU0J_002571"
     CDS             8767..9132
                     /gene="fliT"
                     /locus_tag="ACHU0J_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1217217.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9210..10697
                     /gene="amyA"
                     /locus_tag="ACHU0J_002572"
     CDS             9210..10697
                     /gene="amyA"
                     /locus_tag="ACHU0J_002572"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1217218.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10731..11144)
                     /gene="yedD"
                     /locus_tag="ACHU0J_002573"
     CDS             complement(10731..11144)
                     /gene="yedD"
                     /locus_tag="ACHU0J_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1217219.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11331..12536
                     /gene="yedE"
                     /locus_tag="ACHU0J_002574"
     CDS             11331..12536
                     /gene="yedE"
                     /locus_tag="ACHU0J_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1217220.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            12533..12766
                     /gene="yedF"
                     /locus_tag="ACHU0J_002575"
     CDS             12533..12766
                     /gene="yedF"
                     /locus_tag="ACHU0J_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1217221.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12873..13543
                     /locus_tag="ACHU0J_002576"
                     /pseudo
     CDS             12873..13543
                     /locus_tag="ACHU0J_002576"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(13583..14791)
                     /locus_tag="ACHU0J_002577"
                     /pseudo
     CDS             complement(13583..14791)
                     /locus_tag="ACHU0J_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            14799..15225
                     /gene="tnpA"
                     /locus_tag="ACHU0J_002578"
                     /pseudo
     CDS             14799..15225
                     /gene="tnpA"
                     /locus_tag="ACHU0J_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3738 a         4020 c         3826 g         3876 t
ORIGIN      
        1 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
       61 gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt
      121 gcagcagaag cttctcgagg aactggcggg tgcgaggctg ctggggatcg gcaaataacg
      181 cttttgctgc cccctgctcg actatccgcc cctggtccat aaagatcgcc cggtccgcaa
      241 catcccgggc aaagctcatt tcgtgtgtca caatcaccat cgtgcgcttt tcctgcgcca
      301 gctggcggat ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct
      361 cgtcaaacaa aatcacctca ggacgcattg ccagtgcacg cgcaatcgca acacgctgct
      421 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
      481 ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
      541 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga
      601 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
      661 cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat
      721 ttatgctacg tagcaatgtg gttttgccgg aaccactcgg accgataatt gccaccactt
      781 cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtgg aattttttca
      841 ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
      901 ctcaaaatgg ttctgcaacg tcgataacac agtcgccatg atccagtaga tgagcgaagc
      961 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
     1021 cagctccggc acctggattg tcgcggccag cgacgtatct tttaccagac tgataaagct
     1081 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt
     1141 ctgccacggc gtcataccaa tactggcagc ggcttcccac tgacctttat caatagaaga
     1201 aatggcggcg cgcagcgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat
     1261 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
     1321 cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     1381 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagta gtaagccaaa
     1441 aaacatgccg ccgatactga gttgcagggt atacccagcc cctttcaaca ggaacggcaa
     1501 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
     1561 tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg cccttcatct
     1621 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
     1681 ggatcaagca gaatgccttc cagacgcgcc agcaatttca ctgcttccat gccttcgtcg
     1741 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg
     1801 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caattgatcg
     1861 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
     1921 ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata
     1981 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg
     2041 ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
     2101 cgcgtcgcca gttcttccag ttgggcattg ggatcggtca gtgcgtcgca catttcaatc
     2161 tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt
     2221 gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcgactgcg
     2281 gcagtctggc gcacatggtt agactggatc gcgccggcag taatcagcgt atcggcacct
     2341 tcgcgcaggg catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc
     2401 atgggggtga cctcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc
     2461 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
     2521 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
     2581 gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt
     2641 atttggtcac atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt
     2701 gcatttccgc aattgcatca ttcaccgctt tcagcaggtc ttcatttcct ttacgcagcg
     2761 ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
     2821 tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata cgccctacgc
     2881 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgaca ccctgaacat
     2941 tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
     3001 gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg
     3061 aaatggtgta aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct
     3121 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg
     3181 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
     3241 caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa gtgccttcca
     3301 gccctaccag cagcgtgccg ctctctttga ctttattaag cagaccttca tctgcaaaac
     3361 ttttaacgct catgcccgca accagcgcca cggccatcac acccatcaat gcctgacgtc
     3421 ccagatgtgc taatttcata ttcaccccga atgttgttat gtctgtttgc agtgtagagc
     3481 cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag
     3541 aagcaggcag gctggaggat ttctgcacaa gtccggtaca ggtttggcgc tgatagtgtt
     3601 gatacttccg taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga
     3661 ggatttcatc aaggaaaccg tcctgcagct gatcgaggga aatattttgc tcatgtagat
     3721 gattacccaa acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag
     3781 tttgctcaaa aagaaatttc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc
     3841 aatgtaaatc accgcaaaat cggcacaatg ccgcccatga ttgttgttcc gtttgccagc
     3901 ctttttcatc aagcagcgtg tccaggcggg aaatctcgat tttattcact attttgccgt
     3961 cgcgaactaa ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt
     4021 gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta
     4081 agaactcctg gtagtcaaag ttaaagtgcg gcatttactg acgttataac ttacccagtt
     4141 tagtgcgtaa ccgtttaatg tcctggctgt gtaactgact gacccgcgat tccccgacct
     4201 ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca
     4261 gtttttcgcg ctccggcaac gtttcgatgg cttccatcac ccgctggcgc agattactgt
     4321 ccagcagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat
     4381 cgccgtgctc ttcacgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca
     4441 gcatttgacg ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg
     4501 cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc
     4561 gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga
     4621 tacgctacac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacag
     4681 cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca
     4741 gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg
     4801 agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt
     4861 tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg
     4921 gtgaaatgag gggttatttg ggggttacgc aaaaagccga atgtaaaaaa ccccgccggt
     4981 ggcggggttt gagaggcatt cagacgatta accctgcagc agagacagaa cctgctgcgg
     5041 tacctggttg gcttttgcca gcacggagtt accggcctgc tggataatct gcgctttcga
     5101 catgttggac acttcggtcg catagtcggc gtcctgaata cgggactgcg cttcagacag
     5161 gttggtagtg gtgttgttca ggttggtgac tgcggaatcc aaacggtttt gcaccgcacc
     5221 cagggaggag cggaatttgt cgatggagct gatggcttca tccagagctt tcaggggatc
     5281 ggctgtcgta gctgcttttg tttcagcatc tgtcgtcaat ttaccatcag catctttata
     5341 aactgcctta ccgctgccgt tagtcactga accatcatcc tgtaggtaaa aatctacagt
     5401 atcagatgtg gtcaacgcct cgttgttatc aacataaacc gaatcaacac cttttgttac
     5461 agcgccagtt gtgccgttga cgctataacc tgcagtagtg aaatcaacat tgtttcccga
     5521 ggttgataca gctttatcga aagctgcctg atcaactttt gcacctgtta ctgaataagt
     5581 aatagtgtcc ggcgtagtac tattaccggt aaaggagata gaggtaccgc caatgtcaat
     5641 ggttgtcgct ttggtattat tggctgcaag tgtggacaga ctagcctcag tcaaagaagc
     5701 accagagccg tttttcgtta agcgcccagt tgtatcaatg tagagtttat ctccattgct
     5761 tgccgtaatt ttaccatcac tgctgatctg aacatcctgt gcagaaccat cgatagtaat
     5821 agtgccctta gcggtatcac caactcccgg ggtcaaatat ttctgaacat cggcagctga
     5881 agccgttgtg gtatcaaaag agtatgactt acttgcactg tcatacttgt aattcgttgc
     5941 actagccgcc gttccgaagc cgttattaat ggttgcctga acagtatcac catcactaac
     6001 tccagccagc aaatcagacg ctttagcagc atcgtaaccc gcactcacag tatatttgtt
     6061 gccgaccact gttgcattag cagctaccaa gtcagcttta gatgcagcag tattagccac
     6121 agccccgcca ccattcacat taaacccatt cagccccagc gtatcagagt caattttctt
     6181 cagatcaata gtgatagtct ggccgtcatt cgcaccaacc tgaattttca tcgaaccgtc
     6241 ttttgccagt acgttcacgc cgttgaactg ggtctgaccg gatacgcggt caatttcgtc
     6301 gagacgggat ttgatttcgt cctgaatgga gtccagatcc gaatcagagt tagtcccggt
     6361 agaagcctga accgtcagct cacggatacg ctgtaagttg ttgttgattt cggacagcgc
     6421 gccttcagtg gtctgtgcaa cagaaatacc gtcgttggcg ttacgtgcag cctgagtcag
     6481 gcctttaatg ttagaagtaa aacggttagc aatcgcctga cccgcggcgt catccttcgc
     6541 gctgttaata cgcaagccag aagacagacg ctcgatagaa ctcgacagcg cagactggtt
     6601 cttgttgata ttattttgag tgatcagcga gaggctgttg gtattaatga cttgtgccat
     6661 gattcgttat cctatattgc aagtcgttga ttacgttttg ggtttccacc cgtcggctca
     6721 atcgccgtca accctgttat cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac
     6781 agccattttt tgttagtcga cgaaatactc ttttctctgc cccttattcc cgctattaaa
     6841 aaaaacaatt aaacgtaaac tttgcgcaat tcaggccgat aaccccggta ttcgttttac
     6901 gtgtcgaaag ataaaaggaa atcgcatggc aagtatttca tcgctgggag tcgggtcagg
     6961 tctggattta agttcaatcc ttgatagcct caccgccgcg caaaaagcga cgctaacccc
     7021 tatttcaaat cagcaatcgt cgtttaccgc taaacttagc gcctacggta cgctgaaaag
     7081 cgcgctgacg actttccaga ccgccaatac tgcattgtct aaagccgatc ttttttccgc
     7141 caccagcacc accagcagca ccaccgcgtt cagtgccacc accgcgggta acgccatcgc
     7201 cgggaaatac accatcagcg tcacccatct ggcgcaggcg caaaccctga ccacgcgcac
     7261 caccagagat gatacgaaaa cggcgatcgc caccagcgac agcaaactca ccattcaaca
     7321 aggcgacgac aaagatccga ttaccattga tatcagcgcg gctaactcat cgttaagcgg
     7381 gatccgtgat gccatcaaca acgcaaaagc gggcgtaagt gcgagcatca ttaacgtggg
     7441 taacggtgaa tatcgtctgt cagtcacatc aaatgacacc ggccttgata atgcgatgac
     7501 actctcggtc agcggtgatg atgcgctaca aagttttatg ggctatgacg ccagtgccag
     7561 cagcaacggt atggaggtct cggtcgccgc ccagaatgcg cagctgacgg tcaacaacgt
     7621 cgccatcgag aacagcagca acactatcag cgacgcgctg gaaaacatca ctctgaacct
     7681 gaacgatgtc accacgggca accagacgct aaccatcact caggacacct ccaaagtgca
     7741 aacggcgatt aaagactggg tgaatgccta taactcgcta atagatacct tcagcagcct
     7801 gaccaaatac accgccgtag atgcgggagc tgatagccag agttctagca atggcgcact
     7861 gctcggcgac tccacgctgc ggacgattca gacgcagttg aagtcgatgc tgagtaatac
     7921 cgtcagttct tccagctata aaacgctggc gcagattggt atcacgaccg atcccagcga
     7981 tggcaaactg gaactggatg ccgacaaact caccgctgca ctgaaaaaag atgccagcgg
     8041 cgtaggtgca ttgattgttg gcgatggtaa aaaaaccggc atcacaacca ccatcggcag
     8101 caacctgacc agttggcttt cgacaacggg cattattaaa gccgctaccg atggcgttag
     8161 taaaaccctg aataagttaa ctaaagacta caacgccgcc agcgatcgca ttgatgcgca
     8221 ggtcgcgcgc tacaaagaac aatttaccca actggacgtt ttaatgacct cgttaaacag
     8281 caccagcagc tacttaacgc agcagttcga aaacaacagt aattccaagt aatcattatt
     8341 catcgggaga caggtcatgt acgcggcaaa aggcacccag gcctatgcac aaattggcgt
     8401 cgaaagcacc gtaatgagcg ccagccaaca gcagttggtc accatgctat ttgatggagt
     8461 gctgagcgca ctggttagag cgcgactgtt tatgcaggac aacaatcagc aaggcaaagg
     8521 cgtctctttg tcaaaagcga tcaacatcat tgagaacgga ctgcgggtga gtcttgatga
     8581 agagagcaaa gacgaattaa cccaaaactt aattgctctt tatagctata tggtcaggcg
     8641 tttgctgcaa gccaatttac gcaacgatgt ctcagcagtc gaagaggtgg aagcattaat
     8701 gcgtaatatt gccgatgcct ggaaagagtc tttactctcc ccttcttcga ttcaggaccc
     8761 agtctgatga acaatgcacc gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc
     8821 cagctcatgt tacgcctggc aacggaagaa caatgggacg aactcatcgc tagcgaaatg
     8881 gcgtatgtga atgcggtgca ggagatcgca cacctgactg aagaggttgc cccgtccact
     8941 acgatgcagg agcagctccg cccgatgctg cacctgattc tcgacaacga aagcaaggta
     9001 aagcagttat tacagattcg gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg
     9061 caaaaatcgg tgttaagtgc ctatggcgat cagggcggct ttgtgctggc tccgcaggat
     9121 aacctctttt gaatcttaat gagccgatgg ctcgcgaata atccgattac ggctacgctt
     9181 ctaatattcc ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc
     9241 actggtatta cccggaaggc ggtaagctct ggcctgaact ggccgagcgc gcagacggtt
     9301 ttaatgatat tggtatcaat atggtctggt tgccgcccgc ctataaaggc gcatcgggcg
     9361 gctattcggt cggctacgac tcctatgatt tatttgatct aggtgagttt gatcagaaag
     9421 gcagcatccc tactaaatat ggtgataaag cacaactgct ggccgccatt gacgccctga
     9481 aacgtaatga cattgcggtg ctgctggacg tggtagtcaa ccacaaaatg ggcgcggatg
     9541 aaaaagaagc tattcgcgtg cagcgtgtaa atgctgatga ccgtacgcaa attgacgaag
     9601 aaatcattga gtgtgaaggc tggacgcgtt acaccttccc cgctcgtgcc gggcaatact
     9661 cgcagtttat ctgggatttc aaatgtttta gcggtatcga ccatatcgaa caccctgatg
     9721 aagatggcat ttttaaaatt gttaacgact ataccggaga aggctggaac gatcaggttg
     9781 atgatgaact cggtaatttc gattatctga tgggtgagaa tatcgatttt cgcaatcatg
     9841 ccgtgacgga agagattaaa tactgggcgc gctgggtgat ggaacaaacg caatgcgacg
     9901 gctttcgtct tgatgcggtc aaacatattc cggcatggtt ttataaagag tggatcgaac
     9961 acgtacagga agttgcgcca aagccgctgt ttattgtggc tgaatgctgg tcgcatgaag
    10021 tcgataaact gcaaacgtat attgatcagg tggaaggcaa aaccatgctg tttgatgcgc
    10081 cgctgcagat gaaattccat gaagcatcgc gcatgggtcg cgactacgac atgacgcaga
    10141 tcttcactgg gacgctggtg gaagccgatc cttttcacgc cgtaaccctc gttgccaacc
    10201 acgacaccca accgttgcaa gccctcgaag caccggtcga accgtggttt aaaccgctgg
    10261 cgtatgcctt aattttgttg cgggaaaatg gcgttccttc ggtgttctat ccggacctct
    10321 acggtgcaca ttacgaagat gtcggtggtg acgggcaaac ctatccgata gatatgccaa
    10381 taatcgaaca gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga
    10441 cattattttt cgaccatccg aactgcattg cctttagccg cagtggcacc gacgaatatc
    10501 ccggctgcgt ggtggtcatg tcgaacggag atgatggcga aaaaaccatt catctgggag
    10561 agaattacgg caataaaacc tggcgtgatt ttctggggaa ccggcaagag agcgtagtga
    10621 cagacgaaaa cggcgaagcg accttctttt gcaacggcgg cagcgtcagc gtgtgggtta
    10681 tcgaagaggt gatttaaatc catccccggc ggcaagccgg ggagatttca ttacggcagt
    10741 ggcgtcggta acggcgcttt atccagcgcg gcggcacact ctgccgtcgg gcgatccaca
    10801 cgctccatcg tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc
    10861 tcacgtttta cggtaacgtt agtgagatca tcagacatca atgtcaactt acccggcacg
    10921 gcaatccccc gctgccactg acggcaatcc aaggtatcgc cctctttagt gacaatcagg
    10981 ctgcctatcg cttccggact gaccagcgca cgttgcggcc ctttggtctg ccagtagccc
    11041 gccagccagt ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag
    11101 cccgccagca acatgagcgc gcctgcaatt gcgagttttt tcatcattcg tcctgcatga
    11161 gaagaaaaga ggattgtggc attaaagctc tgatgtcgcc agcctttcca ggttcagaga
    11221 gaaatttgat ctaactatta tttttgtgaa ttattacccc gaaaataaat ttgcatggtg
    11281 tatgattcgc ggctcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc
    11341 agcaattcaa acacgcctgg ttgattaaat tctgggcacc catccctgcg gtcatcgcgg
    11401 cgggtattct ctccacttac tattttggca ttactggcac cttttgggct gtcacgggtg
    11461 aatttacccg ttggggcggt cagctcctgc agctgtttgg cgtccatgct gaagagtggg
    11521 gttattttaa aattatccat ctggaaggat cgccattaac ccggatcgac ggcatgatga
    11581 tcctcggtat gtttggcggc tgctttgctg ctgcgctgtg ggccaacaat gtcaaactac
    11641 gcatgccacg cagccgtatc cgcattatgc aggccatcat tggcggcatt atcgccggtt
    11701 ttggcgcgcg tctggcaatg ggctgtaacc tggcggcgtt ctttaccggc attcctcagt
    11761 tctcgctgca tgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcgc
    11821 gctttaccct gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct
    11881 caccgctgac gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct
    11941 ttttcggcat gctgggctgg gcgctgctca cggcgatgaa ccaaccaaaa ctggggctgg
    12001 caatgctgtt tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc tgctttactt
    12061 cggcgttccg cgatatgtgg atcaccggac gtacccatat ggcgaaagca atcattatcg
    12121 gtatggcggt gagtgccatc gggatcttca gttacgtaca gttaggcgtt gaacctaaaa
    12181 tcatgtgggc gggaccaaat gcggtaattg gtggtttact ttttggtttt ggcatcgtgc
    12241 tggcaggcgg ctgcgaaacc ggctggatgt accgcgcggt agaaggccag gtgcactact
    12301 ggtgggtcgg tctgggcaac gtgatcggct caacgattct ggcgtattac tgggatgatt
    12361 tcgctccggc gctggccacc gactgggaca aaatcaacct gctgaaaact tttggtccga
    12421 tgggaggcct gctggtgaca tatttgctgc tgtttgctgc gctgatgttg attatcggct
    12481 gggaaaaacg cttcttccgc cgtgaggcac cgcagactgc taaggagatc gcatgaaaaa
    12541 tatcgttcct gattaccgcc ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc
    12601 aacccttgag gcgatgccgc aattaaaaaa aggtgagatc ctggaagtgg tgagcgactg
    12661 tccgcagtcg atcaataata ttccactgga tgcgcgtaat cacggctaca cggtgctgga
    12721 tattcagcaa gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc
    12781 tgtggcccac agcgggaaca tcgttacctc tgacacatag attttacccg ccccctctcc
    12841 tattcttttc agtatgatct caggaggttg ctatgtgtgg acgctttgcc caatcccaaa
    12901 cgcgtgagga ttaccttgcg cttctcgcgg aagatattga acgcgatatt ccctacgatc
    12961 ccgaacccat tggcagatac aatgtcgcgc cgggaaccaa agttcttctg ctcagtgaac
    13021 gtgatgaaca ccttcatctg gatccggttt tctggggata tgctcccgga tggtgggata
    13081 aaccgccgct gattaacgcc cgcgtagaaa ctgcctcaac cagtcgcatg tttaaaccgc
    13141 tctggcaaca tggtcgggca atctgttttg ccgatggctg gtttgagtgg aaaaaagaag
    13201 gcgacaaaaa aagccttatt ttatctatcg cgctgacgga caacctgttt ttatggccgc
    13261 gataggtagc ataccgtttg agcgtggtga cgaagccgaa ggatttttga tcgtcactgc
    13321 ggcggcagat caaggtctgg tagatattca tgaccgccgc ccgctggttc tgtcgccaga
    13381 agctgcacgg gaatggatgc ggcaagacat tggtggtaaa gaagcctcag aaatcgctac
    13441 cagaagctgt gtgccagcaa accagttcac ctggcaccct gtgtcgcgca cggtgggtaa
    13501 tgtcaaaaac cagggcgcgg agttaattca acctgtttgt tgacatcctc cacgccctga
    13561 atgacgagga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13621 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13681 aaatgttacg agctccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13741 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc
    13801 ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg ccagagctgc ttgtactcaa
    13861 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13921 cccggacatt gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga
    13981 ccttcaaatc ctcaatgact atcattacgt ggtttttgct gacggtcgtt gtgactttgt
    14041 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
    14101 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
    14161 gcgtcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
    14221 aggcaagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag
    14281 ttgatacttc actttctgcc tgaatactga tgtaccactt accgcaggac tggctgacag
    14341 tgacattttt cacaacaccc gtgacctgac ggctgttccg gtagcgcatc cagccaagtt
    14401 ttggcagaaa aatacggctg ttttcctgag cgagcttaac gccctgcgga tagcggaatg
    14461 catcattctg tcaccgcttt ttgaatcagg ggaacgccga gcgcttctgg aaaaagtttt
    14521 tgcaggcctg ctcagggtct ttcagtgact gttgcaatgg ctgtgacgga gcatctttaa
    14581 tccattgcgt ttcagtggca tttttccact caaccaatca ggaagccatt ttcgcgtaag
    14641 ggatgtcttt gttaccggcc ttatgattct cattctgacg tgccagagta cgactgaaaa
    14701 cgaaacgaca tgcgcctgcg aagcgcctca tttcacgcgc ctgttgacca ccgggtctta
    14761 accggaattt gaatacctgt agtcgtttca cagggcaaat tatactttgg tctgtgaaaa
    14821 aaactgatat ccgacgcgga aggcactgtg ttttcctgat gcatgctcac cgggtctttg
    14881 ttaccaagta ccgacgccag atttttgacc atgacgcgac agaaaaacta cgcacttact
    14941 tttcaaatgt atgtgcttat tttgaagctg aactggttga aatggatggc gaaccagatc
    15001 acgtccatct gttaattaac tatcccccca aactggcgat atccagtctg gtaaacaaac
    15061 agcctcaaag gcgtatcggg taggttactg cgacgggatc gaccagatat tgctgccagg
    15121 tattaccgca aagatgtttt gtggcgtccc ggctattttg ccagtagctg cggaggtgcg
    15181 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtca gatgaaaaaa
    15241 cgagccttat atccccgccc tgaaggacgg gcttttacgg tgcactggat aagcctggag
    15301 attatctaca cctgcatgga catcattgta ctatgccccg tacatgctcc atcacatcct
    15361 caacatcaaa gtattagagc cttgcaaacc attatgcgtc tcagtttgtc tcatctatct
    15421 tacgcaaaaa aaatagaact aatatcaagc ggcgctatat
//
LOCUS       DAZPDE010000093        15079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPDE010000093 DAZPDE010000000
VERSION     DAZPDE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.093"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            312..733
                     /gene="atpI"
                     /locus_tag="ACHU0J_002579"
                     /pseudo
     CDS             312..733
                     /gene="atpI"
                     /locus_tag="ACHU0J_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            742..1557
                     /gene="atpB"
                     /locus_tag="ACHU0J_002580"
     CDS             742..1557
                     /gene="atpB"
                     /locus_tag="ACHU0J_002580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1217222.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1604..1843
                     /gene="atpE"
                     /locus_tag="ACHU0J_002581"
     CDS             1604..1843
                     /gene="atpE"
                     /locus_tag="ACHU0J_002581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1217223.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1905..2375
                     /gene="atpF"
                     /locus_tag="ACHU0J_002582"
     CDS             1905..2375
                     /gene="atpF"
                     /locus_tag="ACHU0J_002582"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1217224.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2390..2923
                     /gene="atpH"
                     /locus_tag="ACHU0J_002583"
     CDS             2390..2923
                     /gene="atpH"
                     /locus_tag="ACHU0J_002583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1217225.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2936..4477
                     /gene="atpA"
                     /locus_tag="ACHU0J_002584"
     CDS             2936..4477
                     /gene="atpA"
                     /locus_tag="ACHU0J_002584"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1217226.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4528..5391
                     /gene="atpG"
                     /locus_tag="ACHU0J_002585"
     CDS             4528..5391
                     /gene="atpG"
                     /locus_tag="ACHU0J_002585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1217227.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5418..6800
                     /gene="atpD"
                     /locus_tag="ACHU0J_002586"
     CDS             5418..6800
                     /gene="atpD"
                     /locus_tag="ACHU0J_002586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1217228.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6821..7240
                     /locus_tag="ACHU0J_002587"
     CDS             6821..7240
                     /locus_tag="ACHU0J_002587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1217229.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7591..8961
                     /gene="glmU"
                     /locus_tag="ACHU0J_002588"
     CDS             7591..8961
                     /gene="glmU"
                     /locus_tag="ACHU0J_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1217230.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9123..10952
                     /gene="glmS"
                     /locus_tag="ACHU0J_002589"
     CDS             9123..10952
                     /gene="glmS"
                     /locus_tag="ACHU0J_002589"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1217231.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            11114..11935
                     /locus_tag="ACHU0J_002590"
     CDS             11114..11935
                     /locus_tag="ACHU0J_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1217232.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            11922..14030
                     /locus_tag="ACHU0J_002591"
     CDS             11922..14030
                     /locus_tag="ACHU0J_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1217233.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            14027..>15079
                     /locus_tag="ACHU0J_002592"
     CDS             14027..>15079
                     /locus_tag="ACHU0J_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1217234.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLF"
BASE COUNT         3808 a         3493 c         4000 g         3778 t
ORIGIN      
        1 aggcatcatt tgccaagtaa ataaatatgc tgtgcgcgaa catgcgcaat atgtgatctg
       61 aagcacgctt tatcaccagt gtttacgcgt tatttacagt ttttcatgat cgaacagggg
      121 tagcggaaaa gtcgcaattg tatgcactgg aaaaatattt aaacatttat tcaccttttg
      181 gctacttatt gtttgaaatc acgggggcgc accgtataat ttgaccgctt tttgatgctt
      241 gactctaagc cttaaagaaa gttttatacg acacgcggca tacctcgaag ggagcaggag
      301 tgaaaaacgt gatgtctgtg tcgctcgtga gtcgaaacgt tgctcggaag cttctgctcg
      361 ttcagttact ggtggtgata gcaagtggat tgctgttcag cctcaaagac cccttctggg
      421 gcgtctctgc aataagcggg ggcctggcag tctttctgcc taacgttttg tttatgatat
      481 ttgcctggca gtctttctgc ctaacgtttt gtttatgata tttgcctggc gtcaccaggc
      541 gcatacacca gcgaaaggcc gggtggcttg gacattcgca tttggcgaag ctttcaaagt
      601 tctggcgatg ttggtgttac tggtggtggc gttggcggtt ttaaaggcgg tattcttgcc
      661 gctgatcgtt acgtgggttt tggtgctggt ggttcagata ctggcaccgg ctgtaattaa
      721 caacaaaggg taaaaggcat catggcttca gaaaatatga cgccgcagga ttacatagga
      781 caccacctga ataaccttca gctggacctg cgtacattct cgctggtgga tccacaaaac
      841 cccccagcca ccttctggac aatcaatatt gactccatgt tcttctcggt ggtgctgggt
      901 ctgttgttcc tggttttatt ccgtagcgta gccaaaaagg cgaccagcgg tgtgccaggt
      961 aagtttcaga ccgcgattga gctggtgatc ggctttgtta atggtagcgt gaaagacatg
     1021 taccatggca aaagcaagct gattgctccg ctggccctga cgatcttcgt ctgggtattc
     1081 ctgatgaatc tgatggattt actgcctatc gacttgctgc cgtacattgc tgaacatgta
     1141 ctgggtctgc ctgcactgcg tgtggttccg tctgcggacg tgaacgtaac gctgtctatg
     1201 gcactgggcg tatttatcct gattctgttc tacaacatca aaatgaaagg catcggcggc
     1261 ttcacgaaag agttgacgct gcagccgttc aatcactggg cgttcattcc tgtcaactta
     1321 atccttgaag gggtaagcct gctgtccaaa ccagtttcac tcggtttgcg actgttcggt
     1381 aacatgtatg ccggtgagct gattttcatt ctgattgctg gtctgttgcc gtggtggtca
     1441 cagtggatcc tgaatgtgcc gtgggccatt ttccacatcc tgatcattac gctgcaagcc
     1501 ttcatcttca tggttctgac gatcgtctat ctgtcgatgg cgtctgaaga acattaattt
     1561 accaacacta ctacgtttta actgaaacaa actggagact gtcatggaaa acctgaatat
     1621 ggatctgctg tacatggctg ccgctgtgat gatgggtctg gcggcaatcg gtgctgcgat
     1681 cggtatcggc atcctcgggg gtaaattcct ggaaggcgca gcgcgtcaac ctgatctgat
     1741 tcctctgctg cgtactcagt tctttatcgt tatgggtctg gtggatgcta tcccgatgat
     1801 cgctgtaggt ctgggtctgt acgtgatgtt cgctgtcgcg tagtaagcgt tgcttttatt
     1861 taaagagcaa tatcagaacg ttaactaaat agaggcattg tgctgtgaat cttaacgcaa
     1921 caatcctcgg ccaggccatc gcgtttgtcc tgttcgttct gttctgcatg aagtacgtat
     1981 ggccgccatt aatggcagcc atcgaaaaac gtcaaaaaga aattgctgac ggccttgctt
     2041 ccgcagaacg agcacataag gaccttgacc ttgcaaaggc cagcgcgacc gaccagctga
     2101 aaaaagcgaa agcggaagcc caggtaatca tcgagcaggc gaacaaacgc cgctcgcaga
     2161 ttctggacga agcgaaagct gaggcagaac aggaacgtac taaaatcgtg gcccaggcgc
     2221 aggcagaaat tgaagccgag cgtaaacgtg cccgtgaaga gctgcgtaag caagttgcta
     2281 tcctggctgt tgctggcgcc gagaagatca tcgaacgttc cgtggatgaa gctgctaaca
     2341 gcgacatcgt ggataaactt gtcgctgaac tgtaaggagg gaggggctga tgtctgaatt
     2401 tattacggta gctcgcccct acgccaaagc agcttttgac tttgccgtcg aacaccaaag
     2461 tgtagaacgc tggcaggaca tgctgacgtt tgccgccgag gtaaccaaaa acgaacaaat
     2521 ggcagagctt ctctctggcg cgcttgcgcc agaaacgctc gccgagtcgt ttatcgcagt
     2581 ttgtggtgag caactggacg aaaacggtca gaacctgatt cgggtaatgg ctgaaaatgg
     2641 tcgtcttaac gcgctcccgg atgttctgga gcagtttatt caccttcgtg ccgtgagtga
     2701 ggctaccgct gaggtagacg tcatttccgc tgccgcactg agtgaacaac agctcgcgaa
     2761 aatttctgct gcgatggaaa aacgtctgtc acgcaaagtt aagctgaatt gcaaaatcga
     2821 taagtctgta atggctggcg ttatcatccg agcgggtgat atggtcattg atggcagcgt
     2881 acgcggtcgt cttgagcgcc ttgcagacgt cttgcagtct taaggggact ggagcatgca
     2941 actgaattcc accgaaatca gcgaactgat caagcagcgc attgctcagt tcaatgttgt
     3001 gagtgaagct cacaacgaag gtactattgt ttctgtaagt gacggtgtta tccgcattca
     3061 cggcctggcc gattgtatgc agggtgaaat gatctccctg ccgggtaacc gttacgctat
     3121 cgcactgaac ctcgagcgcg actctgtagg tgcggttgtt atgggtccgt acgctgacct
     3181 tgccgaaggc atgaaagtta agtgtactgg ccgtatcctg gaagttccgg ttggccgtgg
     3241 cctgctgggc cgtgtggtta acactctggg tgcaccaatc gacggtaaag gtccgctgga
     3301 tcacgacggc ttctctgctg tagaagcaat cgctccgggc gttatcgaac gtcagtccgt
     3361 agatcagccg gtacagaccg gttataaagc cgttgactcc atgatcccaa tcggtcgtgg
     3421 tcagcgtgaa ttgatcatcg gtgaccgtca gacaggtaaa accgcactgg ctatcgatgc
     3481 catcatcaac cagcgcgatt ccggtatcaa atgtatctat gtcgctatcg gccagaaagc
     3541 gtccaccatt tctaacgtgg tacgtaaact ggaagagcac ggcgcactgg ctaacaccat
     3601 cgttgtggta gcaaccgcgt ctgaatccgc tgcactgcaa tacctggcac cgtatgccgg
     3661 ttgcgcaatg ggcgaatact tccgtgaccg cggtgaagat gcgctgatca tttacgatga
     3721 cctgtctaaa caggctgttg cttaccgtca gatctccctg ctgctccgtc gtccgccagg
     3781 acgtgaagca ttcccgggcg acgttttcta cctccactct cgtctgctgg agcgtgctgc
     3841 acgtgttaac gccgaatacg ttgaagcctt caccaaaggt gaagtgaaag ggaaaaccgg
     3901 ttctctgacc gcactgccga ttatcgaaac tcaggcgggt gacgtttctg cgttcgttcc
     3961 gaccaacgta atctccatta ccgatggtca gatcttcctg gaaaccaacc tgttcaacgc
     4021 cggtattcgt cctgcggtta acccgggtat ttccgtatcc cgtgttggtg gtgcagcaca
     4081 gaccaagatc atgaaaaaac tgtccggtgg tatccgtacc gctctggcac agtatcgtga
     4141 actggcagcg ttctctcagt ttgcatccga ccttgacgat gcaacacgta agcagcttga
     4201 ccacggtcag aaagtgaccg aactgctgaa acagaaacag tatgcgccga tgtccgttgc
     4261 gcagcagtct ctggttctgt tcgcagcaga acgtggttac ctggcggatg ttgaactgtc
     4321 gaaaattggc agcttcgaag ccgctctgct ggcttacgtc gaccgtgatc acgctccgtt
     4381 gatgcaagag atcaaccaga ccggtggcta caacgacgaa atcgaaggca agctgaaagg
     4441 catcctcgat tccttcaaag caacccaatc ctggtaacgt ctggaggctt gccttagggc
     4501 aggccgcaag gcattgagga gaagctcatg gccggcgcaa aagagatacg tagtaagatc
     4561 gcaagcgtcc agaacacgca aaagatcact aaagcgatgg agatggtcgc cgcttccaaa
     4621 atgcgtaaat cgcaggatcg catggcggcc agccgtcctt atgcagaaac catgcgcaaa
     4681 gtgattggtc accttgcaca cggtaatctg gaatataagc acccttacct ggaagaccgc
     4741 gacgttaaac gcgtgggcta cctggtggtg tcgaccgacc gtggtttgtg cggtggtttg
     4801 aacattaacc tgttcaaaaa actgctggcg gaaatgaaga cctggaccga caaaggcgtt
     4861 caatgcgacc tcgcaatgat cggctcgaaa ggcgtgtcgt tcttcaactc cgtgggcggc
     4921 aatgttgttg cccaggtcac cggcatgggg gataaccctt ccctgtccga actgatcggt
     4981 ccggtaaaag tgatgttgca ggcctacgac gaaggccgtc tggacaagct ttacattgtc
     5041 agcaacaaat ttattaacac catgtctcag gttccgacca tcagccagct gctgccgtta
     5101 ccggcatcag atgatgatga tctgaaacat aaatcctggg attacctgta cgaacccgat
     5161 ccgaaggcgt tgctggatac cctgctgcgt cgttatgtcg aatctcaggt ttatcagggc
     5221 gtggttgaaa acctggccag cgagcaggcc gcccgtatgg tggcgatgaa agccgcgacc
     5281 gacaatggcg gcagcctgat taaagagctg cagttggtat acaacaaagc tcgtcaggcc
     5341 agcattactc aggaactcac cgagatcgtc tcgggggccg ccgcggttta aacaggttat
     5401 ttcgtagagg atttaagatg gctactggaa agattgtcca ggtaatcggc gccgtagttg
     5461 acgtcgaatt ccctcaggat gccgtaccgc gcgtgtacga tgctcttgag gtgcaaaatg
     5521 gtaatgagcg tctggtgctg gaagttcagc agcagctcgg cggcggtatc gtgcgtacca
     5581 tcgcaatggg ttcctccgac ggtctgcgtc gcggtctgga tgtaaaagac ctcgaacacc
     5641 cgatcgaagt cccggtaggt aaagcgactc tgggccgtat catgaacgta ctgggtgaac
     5701 cggtcgacat gaaaggcgag atcggtgaag aagagcgttg ggcgattcac cgcgcagcac
     5761 cttcctacga agagctgtca aactctcagg aactgctgga aaccggtatc aaagttatcg
     5821 acctgatgtg tccgttcgct aagggcggta aagttggtct gttcggtggt gcgggtgtag
     5881 gtaaaaccgt aaacatgatg gagctcattc gtaacatcgc gatcgagcac tccggttact
     5941 ctgtgtttgc gggcgtaggt gaacgtactc gtgagggtaa cgacttctac cacgaaatga
     6001 ccgactccaa cgttatcgac aaagtatccc tggtgtatgg ccagatgaac gagccgccgg
     6061 gaaaccgtct gcgcgttgct ctgaccggtc tgaccatggc tgagaaattc cgtgacgaag
     6121 gtcgtgacgt tctgctgttc gttgacaaca tctatcgtta caccctggcc ggtacggaag
     6181 tatccgcact gctgggccgt atgccttcag cggtaggtta tcagccgacc ctggcggaag
     6241 agatgggcgt tctgcaggaa cgtatcacct ccaccaaaac tggttctatc acctccgtac
     6301 aggcggtata cgtacctgcg gatgacttga ctgacccgtc tccggcaacc acctttgcgc
     6361 accttgacgc aaccgtggta ctgagccgtc agatcgcgtc tctgggtatc tacccggccg
     6421 ttgacccgct ggactccacc agccgtcagc tggacccgct ggtggttggt caggaacact
     6481 acgacaccgc gcgtggcgtt cagtccatcc tgcaacgtta tcaggaactg aaagacatca
     6541 tcgccatcct gggtatggat gaactgtctg aagaagacaa actggtggta gcgcgtgctc
     6601 gtaagatcca gcgcttcctg tcccagccgt tcttcgtggc agaagtattc accggttctc
     6661 cgggtaaata cgtctccctg aaagacacca tccgtggctt taaaggcatc atggaaggcg
     6721 aatacgatca cctgccggag caggcgttct acatggtcgg ttccatcgaa gaagctgtgg
     6781 aaaaagccaa aaaactttaa cgccttaatc ggagggtgat atggcaatga cttaccacct
     6841 ggacgtcgtc agcgcagagc aacaaatgtt ctctggtctg gtcgagaaaa tccaggtaac
     6901 gggtagcgaa ggtgaactgg ggatctaccc tggccacgca ccgctgctca ccgccattaa
     6961 gcctggtatg attcgcatcg tgaaacagca cggtcacgaa gagtttatct atctgtctgg
     7021 cggcattctt gaagtgcagc ctggcaacgt gaccgttctg gccgacaccg caattcgcgg
     7081 ccaggatctc gacgaagcgc gagccatgga agcgaaacgt aaggctgaag agcacattag
     7141 cagctctcac ggcgacgtag attacgctca ggcgtctgcg gaactggcca aagcgatcgc
     7201 gcagctgcgc gttatcgagt tgaccaaaaa agcgatgtaa caccggcttg aaaagcacaa
     7261 aagccagtct ggaaacaggc tggctttttt tacgcgttga cccgtcctga agagcgttca
     7321 catagatcct gctgatataa aacacccctg ttttcccgtt tattcattga tcgaaataag
     7381 agcaaaaaca tccacctgac gcttaaatga aggtactgcc ttaattttct gcagataaaa
     7441 ggcgtgacga tggtcgaaaa tggcactttc gtcagcgggg ataatccgtt attgaacaat
     7501 ttatcctctg tccatttcac gatgaaaaaa atgtagtttt ttcaaggtga agcggtttaa
     7561 attcgttctc aaattacagt caggacgcgt atgttgaata atgctatgag cgtagtgatc
     7621 cttgccgcag gcaaaggcac gcgcatgtat tccgatcttc cgaaagtgct gcataccctt
     7681 gccgggaaag cgatggttca gcatgtcatt gatgctgcga atgaattagg cgcagcgcac
     7741 gttcacctgg tgtacggtca cggcggcgat ctgcttaaac aggcgctgaa agacgacaac
     7801 cttaactggg tgcttcaggc agagcagctg ggtacgggtc atgcaatgca gcaggccgca
     7861 cctttctttg ccgatgatga agacatttta atgctctacg gcgacgtgcc gctgatctct
     7921 gtcgaaacac tccagcgcct gcgtgatgct aaaccccagg gtggcattgg tctgctgacg
     7981 gtgaaactgg atgatccgac cggttatgga cgtatcaccc gtgaaaatgg caaagttacc
     8041 ggcattgttg agcacaaaga tgccaccgac gagcagcgtc agattcagga gatcaacacc
     8101 ggcattctga ttgccaacgg cgcagatatg aaacgctggc tggcgaagct gaccaacaat
     8161 aatgctcagg gcgaatacta catcaccgac attattgcgc tggcgtatca ggaagggcgt
     8221 gaaatcgtcg ccgttcatcc gcaacgttta agcgaagtag aaggtgtgaa taaccgcctg
     8281 caactctccc gtctggagcg cgtttaccag tccgaacagg ctgaaaaact gctgttagca
     8341 ggcgttatgc tgcgcgatcc ggcgcgtttt gatctgcgcg gtacgcttac tcacgggcgc
     8401 gatgttgaaa ttgatactaa cgttatcatc gagggcaacg tgactctcgg tcatcgcgtg
     8461 aaaatcggca ccggttgcgt gattaaaaac agcgtgattg gcgatgattg cgaaatcagt
     8521 ccgtataccg ttgtggaaga cgcgaatctg gcagcggcct gtactattgg cccgtttgcc
     8581 cgtctgcgtc ctggtgctga gttgctggaa ggtgcacacg ttggtaactt cgttgagatg
     8641 aaaaaagcgc gtctgggtaa aggcacgaaa gccgggcatc tgacttacct gggcgatgcg
     8701 gaaattggcg ataacgttaa catcggcgcg ggaaccatta cctgcaacta cgatggtgcg
     8761 aataaattta agaccattat cggcgacgat gtgtttgtcg gttccgacac tcagctggtg
     8821 gccccggtaa cagtaggcaa aggcgcgacc attgctgcgg gtacaactgt gacgcgtaat
     8881 gtcggcgaaa atgcattagc tatcagccgt gtgccgcaga ctcagaaaga aggctggcgt
     8941 cgtccggtaa agaaaaagta attctggccg ggtaatccgg tcatctggga tgaggagata
     9001 acataatctc cctcccacaa gcagtaacta taaaaataac cccactctct acaaggctcg
     9061 gggcgcccga aaaaacgggc atacaggttg accgacaacg atataaatcg gaatcaaaaa
     9121 ctatgtgtgg aattgttggc gcgatcgcgc aacgtgatgt agcagaaatc cttcttgaag
     9181 gtttacgtcg tctggaatac cgcggatatg actctgccgg tctggccgtt gttgatgcgg
     9241 aaggtcatat gactcgcctg cgtcgcctcg gtaaagtcca gatgctggct caggcagcgg
     9301 aagaacatcc tctgcatggc ggcaccggta ttgctcacac tcgctgggcg acccacggtg
     9361 aaccttcaga agctaacgcg catccgcatg tttctgaaca cattgtggtg gtgcataatg
     9421 gcatcatcga aaaccatgaa ccgctgcgtg aagagttaaa agcgcgtggc tataccttcg
     9481 tttctgaaac cgacaccgaa gtgattgccc atctggtgaa ctgggagctg aaacaaggcg
     9541 ggactctgcg tgaggctgtt ctgcgtgcta tcccgcagct gcgcggtgcg tacggtacag
     9601 tgatcatgga ctcccgtcac ccggataccc tgctagcggc acgttctggt agtccgctgg
     9661 tgattggcct gggtatgggc gaaaacttta tcgcttctga ccagctggcg ctgttgccgg
     9721 tgacccgtcg ctttatcttc cttgaagagg gcgatattgc ggaaatcact cgccgttcgg
     9781 taaacatctt cgataaaact ggcgcggaag taaaacgtca ggatatcgaa tccaatctgc
     9841 aatatgacgc gggcgataaa ggtatttacc gtcactacat gcagaaagag atctacgaac
     9901 agccgaacgc gataaaaaac acccttaccg ggcgcatcag ccacggtcag gttgatttaa
     9961 gcgagctggg accgaacgcc gacgaactgc tgtcgaaggt tgagcatatt cagatcctcg
    10021 cctgtggtac ttcttataac tccggtatgg tttcccgcta ctggtttgaa tcgctggcag
    10081 gtattccgtg cgacgtcgaa atcgcctctg aattccgcta tcgtaaatct gccgtgcgtc
    10141 gtaacagcct gatgatcacc ttgtcacagt ctggcgaaac cgcggatacc ctggcgggcc
    10201 tgcgtctgtc gaaagagctg ggttaccttg gttcgctggc aatctgtaac gttccgggtt
    10261 cttctctggt gcgcgaatcc gatctggcgc tgatgaccaa cgcgggtacg gaaatcggcg
    10321 tggcgtccac taaagcattc accactcagt taactgtgct gttgatgctg gtggcgaagc
    10381 tgtctcgcct gaaaggtctg gatgcctcca ttgaacatga cattgtgcat ggtctgcagg
    10441 cgctgccgag ccgtattgag cagatgctgt ctcaggataa acgcattgaa gctctggcag
    10501 aagatttctc tgacaaacat cacgcgctgt tcctgggccg tggcgatcag tacccaatcg
    10561 cgctggaagg cgcattgaag ctgaaagaga tctcttacat tcacgctgaa gcctacgctg
    10621 caggcgagct gaaacacggc ccgctggcgc tgattgacgc ggatatgccg gttatcgtcg
    10681 ttgcaccgaa caacgaactg ttggaaaaac tgaaatccaa cattgaagaa gttcgcgcgc
    10741 gtggcggtca attgtatgtc ttcgccgatc aggatgcggg ttttgtaagt agcgataaca
    10801 tgcacatcat cgagatgccg catgtggaag aggtgattgc accgatcttc tacaccgttc
    10861 cgctgcagct gctggcttac catgtcgcgc tgatcaaagg caccgacgtt gaccagccgc
    10921 gtaacctggc aaaatcggtt acggttgagt aatcactatc atagataaaa aagggctgct
    10981 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
    11041 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
    11101 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
    11161 tattaaagag gggcgtggcc aagggcatgg taaagactat attccatggc taacagtaca
    11221 agaagttcct tcttcaggtc gttcccaccg tatttattct cataagacgg gacgagtcca
    11281 tcatttgcta tctgacttag agcttgctgt ttttctcagt cttgagtggg agagcagcgt
    11341 gctagatata cgcgagcagt tccccttatt acctagtgat accaggcaga ttgcaataga
    11401 tagtggtatt aagcatcctg ttattcgtgg tgtagatcag gttatgtcta ctgatttttt
    11461 agtggactgc aaagatggtc cttttgagca gtttgctatt caagtcaaac ctgcagcagc
    11521 cttacaagac gagcgtacct tagaaaaact agaactagag cgtcgctatt ggcagcaaaa
    11581 gcaaattcct tggttcattt ttactgataa agaaataaat cccgtagtaa aagaaaatat
    11641 tgaatggctt tattcagtga aaacagaaga agtttctgcg gagcttttag cacaactatc
    11701 cccattggcc catatcctgc aagaaaaagg agatgaaaac attatcaatg tctgtaagca
    11761 ggttgatatt gcttatgatt tggagttagg caaaacattg agtgagatac gagccttaac
    11821 cgcaaatggt tttattaagt tcaatattta taagtctttc agggcaaata agtgtgcaga
    11881 tctctgtatt agccaagtag tgaatatgga ggagttgcgc tatgtggcaa attaatgagg
    11941 ttgtgctatt tgataatgat ccgtatcgca ttttggctat agaggatggc caagttgtct
    12001 ggatgcaaat aagcgctgat aaaggagttc cacaagctag ggctgagttg ttgctaatgc
    12061 agtatttaga tgaaggccgc ttagttagaa ctgatgaccc ttatgtacat cttgatttag
    12121 aagagccgtc tgtagattct gtcagcttcc agaagcgcga ggaggattat cgaaaaattc
    12181 ttcctattat taatagtaag gatcgtttcg accctaaagt cagaagcgaa ctcgttgagc
    12241 atgtggtcca agaacataag gttactaagg ctacagttta taagttgtta cgccgttact
    12301 ggcagcgtgg tcaaacgcct aatgcattaa ttcctgacta caaaaacagc ggtgcaccag
    12361 gggaaagacg ttcagcgaca ggaacagcaa agattggccg agccagagaa tatggtaagg
    12421 gtgaaggaac caaggtaacg cccgagattg aacgcctttt taggttgacc atagaaaagc
    12481 acctgttaaa tcaaaaaggt acaaagacca ccgttgccta tagacgattt gtggacttgt
    12541 ttgctcagta ttttcctcgc attccccaag aggattaccc aacactacgt cagtttcgtt
    12601 atttttatga tcgagaatac cctaaagctc agcgcttaaa gtctagagtt aaagcagggg
    12661 tatataaaaa agacgtacga cccttaagta gtacagccac ttctcaggcg ttaggccctg
    12721 ggagtcgtta tgagattgat gccacgattg ctgatattta tttagtggat catcatgatc
    12781 gccaaaaaat cataggaaga ccaacgcttt acattgtgat tgatgtgttt agtcggatga
    12841 tcacgggctt ttatatcggc tttgaaaatc cgtcttatgt ggtggcgatg caggcttttg
    12901 taaatgcttg ctctgacaaa acggccattt gtgcccagca tgatattgag attagtagct
    12961 cagactggcc gtgtgtaggt ttgccagatg tgttgctagc ggaccgtggc gaattaatga
    13021 gtcatcaggt cgaagcctta gtttctagtt ttaatgtgcg agtggaaagt gctccaccta
    13081 gacgtggcga tgctaaaggc atagtggaaa gcacttttag aacactacaa gccgagttta
    13141 agtcctttgc acctggcatt gtagagggca gtcggatcaa aagccatggt gaaacagact
    13201 ataggttaga tgcatctctg tcggtatttg agttcacaca aattattttg cgtacgatct
    13261 tattcagaaa taaccatctg gtgatggata aatacgatcg agatgctgat tttcctacag
    13321 atttaccgtc tattcctgtc cagctatggc aatggggtat gcagcatcgt acaggtagtt
    13381 taagggctgt ggagcaagag cagttgcgag tagcgttact gcctcgccga aaggtctcta
    13441 tttcttcatt tggcgttaat ttgtggggtt tgtattactc ggggtcagag attctgcgtg
    13501 agggttggtt gcagcggagc actgatatag ctagacctca acatttagaa gcggcttatg
    13561 acccagtgct ggttgatacg atttatttgt ttccgcaagt tggcagccgt gtattttggc
    13621 gctgtaatct gacggaacgt agtcggcagt ttaaaggtct ctcattttgg gaggtttggg
    13681 atatacaagc acaagaaaaa cacaataaag ccaatgcgaa gcaggatgag ttaactaaac
    13741 gcagggagct tgaggcgttt attcagcaaa ccattcagaa agcgaataag ttaacgccca
    13801 gtactactga gcccaaatca acacgcatta agcagattaa aactaataaa aaagaagccg
    13861 tgacctcgga gcgtaaaaaa cgtgcggagc atttgaagcc aagctcttca ggtgatgagg
    13921 ctaaagttat tcctttcaac gcagtggaag cggatgatca agaagattac agcctaccca
    13981 catacgtgcc tgaattattt caggatccac cagaaaagga tgagtcatga gtgctacccg
    14041 gattcaagca gtttatcgtg atacgggggt agaggcttat cgtgataatc cttttatcga
    14101 ggccttacca ccattacaag agtcagtgaa tagtgctgca tcactgaaat cctctttaca
    14161 gcttacttcc tctgacttgc aaaagtcccg tgttatcaga gctcatacca tttgtcgtat
    14221 tccagatgac tattttcagc cattaggtac gcatttgcta ctaagtgagc gtatttcggt
    14281 catgattcga ggtggctacg taggcagaaa tcctaaaaca ggagatttac aaaagcattt
    14341 acaaaatggt tatgagcgtg ttcaaacggg agagttggag acatttcgct ttgaggaggc
    14401 acgatctacg gcacaaagct tattgttaat tggttgttct ggtagtggga agacgacctc
    14461 tcttcatcgt attctagcca cgtatcctca ggtgatttac catcgtgaac tcaatgtaga
    14521 gcaggtggtg tatttgaaaa tagactgctc gcataatggt tcgctaaaag aaatctgctt
    14581 gaattttttc agagcgttgg atcgagcctt gggctcgaac tatgagcgtc gttatggctt
    14641 aaaacgtcat ggtatagaaa ccatgttggc tttgatgtcg caaatagcca atgcacatgc
    14701 tttagggttg ttggttattg atgaaattca gcatttaagc cgctctcgtt cgggtggatc
    14761 tcaagagatg ctgaactttt ttgtgacgat ggtgaatatt attggcgtac cagtgatgtt
    14821 gattggtacc cctaaagcac gagagatttt tgaggctgat ttgcggtctg cacgtagagg
    14881 ggcagggttt ggagctatat tctgggatcc tatacaacaa acgcaacgtg gaaagcccaa
    14941 tcaagagtgg atcgctttta cggataatct ttggcaatta cagcttttac aacgcaaaga
    15001 tgcgctgtta tcggatgagg tccgtgatgt gtggtatgag ctaagccaag gagtgatgga
    15061 cattgtagta aaacttttt
//
LOCUS       DAZPDE010000094        15061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPDE010000094 DAZPDE010000000
VERSION     DAZPDE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.094"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHU0J_002593"
     CDS             complement(50..2329)
                     /gene="maeB"
                     /locus_tag="ACHU0J_002593"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1217235.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2618..3568
                     /gene="tal"
                     /locus_tag="ACHU0J_002594"
     CDS             2618..3568
                     /gene="tal"
                     /locus_tag="ACHU0J_002594"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1217236.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3588..5591
                     /gene="tkt"
                     /locus_tag="ACHU0J_002595"
     CDS             3588..5591
                     /gene="tkt"
                     /locus_tag="ACHU0J_002595"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1217237.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5694..6737)
                     /locus_tag="ACHU0J_002596"
     CDS             complement(5694..6737)
                     /locus_tag="ACHU0J_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1217238.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHU0J_002597"
     CDS             complement(6863..7438)
                     /gene="nudK"
                     /locus_tag="ACHU0J_002597"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR1217239.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHU0J_002598"
     CDS             complement(7506..9485)
                     /gene="aegA"
                     /locus_tag="ACHU0J_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1217240.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9691..11408
                     /gene="narQ"
                     /locus_tag="ACHU0J_002599"
                     /pseudo
     CDS             9691..11408
                     /gene="narQ"
                     /locus_tag="ACHU0J_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11610..14723
                     /gene="acrD"
                     /locus_tag="ACHU0J_002600"
     CDS             11610..14723
                     /gene="acrD"
                     /locus_tag="ACHU0J_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1217241.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHU0J_002601"
     CDS             complement(14822..14881)
                     /gene="ypfM"
                     /locus_tag="ACHU0J_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1217242.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3535 a         4002 c         3931 g         3593 t
ORIGIN      
        1 aaaccgctta ccctgaatat tcagggtaag cgtgagagtt aagaatgaat tacagcggtt
       61 gggtttgcgc ttcaaccacg gccagcgcca ccatattgac gatacgacgc accgatgcga
      121 tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac
      181 cttccgagct ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca
      241 tcaccagaat attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg
      301 cttccaccag cgctgcatcg ccgtgcattt caccatcaat catcagatct ggcgcacgat
      361 ccctgaccag ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa
      421 agttggagtg cgacaacaga gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg
      481 ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat
      541 cggcaataaa ggtgttacca ctcggcagca gcagcgcatt cattgcacct gcggtgtgaa
      601 cgccatcgcg ataaccaaag acatttttca ccacactaaa atgttcatga taatcgccca
      661 ccgtaccgca gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg
      721 tcgggttact gatcagcgca cgctgcgcct gttcctgagt gacaccgcga cgcttcatga
      781 tctggaagta ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgattt
      841 caaaatcaac gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt
      901 tcggacgacc gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtcgcat
      961 gcagaacgcg cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct
     1021 gggagaaaat cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga
     1081 tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgcttttg
     1141 cgaccgcagg agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt
     1201 ccggaccaaa gctcagatcc tgaacgccat acgctgaagc caccacttca ctctgttctg
     1261 catgggccag ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg
     1321 ttgcgccaac gtccagcgcg ccacggaaga tgaacgggaa gcaaagaacg ttgttcacct
     1381 ggttcgggta gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca
     1441 gcggcggcag aatttccggt tccgggttcg ccagcgccag gatcattggt gcccgagcca
     1501 ttttcttcac catttcctgg gtcagcactt tcgggccgga acagcccagg aaaatatccg
     1561 caccttcaat cacatcatcg agggtacgtt tgccgtcatc caccaccgca tatgcggctt
     1621 tggtttccgc catatttggc tcacggccct gatagataac gccttttgaa tcgcaaacca
     1681 cgatgttatg tttttgcagc cccagcgcta ccagcaggtt catacaggcg attgctgcgg
     1741 cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc
     1801 cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg
     1861 gaatattcat ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt
     1921 cttcgaggtt gatgccgccg aaggtcggtt cgagcgcggc gacgacttca ataaatttgt
     1981 ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat cccagcgaat ttcttaaata
     2041 gaacgccctt gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca
     2101 gcaccgccgt accgttagag atcaccgcaa ccaggttacc gcgcgcggta tatttgtagg
     2161 cttttagcga gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca
     2221 gcgccagatc gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa
     2281 ctggaaattc atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc
     2341 tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc
     2401 acattattac cctgccggag tctacagact ttgagcaagt ccaaactctc accattaata
     2461 taatgttttg gtaataatcc tataacactg atgttacctg cttaatccag caataccatg
     2521 cctgtctgct atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc
     2581 actcatctaa cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa
     2641 acagttcacc actgtcgtgg cagacagctg cgatattgag tccattcgcc attatcatcc
     2701 ccaggatgcc accactaatc cttcgctgtt actcaaggct gccggattat cacaatatga
     2761 gcatttaata gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca
     2821 ggtggtcgca gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt
     2881 acccggttgc gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat
     2941 tgagaaggcg cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat
     3001 tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg gccgaagagc tggaaaaaga
     3061 aggtattaac tgcaacctga cgctgctgtt ttcttttacc caggcgcggg cctgtgcgga
     3121 agcaggcgtt tttctgattt cgccgtttgt agggcgtatt tatgactggt atcaggcacg
     3181 caagccgatg gacccgtatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat
     3241 ctacgactac tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg
     3301 caccgaacaa atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgct
     3361 gaaggagctg caggaaaaag tttctccagt ggtacgtaaa ttaatcccgc cttctcagac
     3421 gttcccacgc ccagcaccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc
     3481 gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc gctgttgatc aacgcaaact
     3541 ggaagatctt cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag
     3601 accttgccaa tgcgattcgc gcactcagta tggatgctgt acaaaaagcc aattctggtc
     3661 atcccggcgc gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta
     3721 aacataatcc taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc
     3781 acgcgtcgat gctgctctac agtttgctac atctgaccgg ttacgacctg ccgctggaag
     3841 aactgaagaa cttccgtcag ttgcattcga aaaccccagg ccacccggag attggctata
     3901 cgccaggcgt tgaaaccacc accggcccgc ttggacaagg tttggcgaac gccgtcgggc
     3961 tggcgatagc agagcgtaca ctggcggcgc agtttaacca gccagaccat gagatcgtcg
     4021 atcacttcac ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag
     4081 tctgttcgct ggcaggcacg ctgggactag gcaagctgat tggtttttac gatcacaacg
     4141 gtatttccat cgacggtgaa acagaaggct ggtttaccga cgatacggca aaacgttttg
     4201 aagcctatca ctggcatgtg atccatgaaa tcgacggtca cgatccgcag gcggtgaagg
     4261 aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg
     4321 tgattggctt tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccactgg
     4381 gggaagaaga agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga
     4441 tccctaaaga gatttatcac gcctgggatg cccgtgaaaa aggcgaaaaa gcgcagcaga
     4501 gctggaatga gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagtttc
     4561 cccgacggat gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca
     4621 atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta
     4681 acgcttacgg gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctcccagca
     4741 acctgaccat ctggaaaggt tctgtttctc tgaaggaaga tccggcgggc aactacattc
     4801 actacggggt gcgtgaattt ggcatgaccg ctatcgccaa cggcatcgcg caccacggcg
     4861 gctttgtgcc gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc
     4921 ggatggcggc actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggcc
     4981 tgggcgaaga tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc
     5041 caaatttcag cacctggcga ccgtgcgatc agatggaagc ggcggtgggc tggaagctgg
     5101 cggttgagcg ccacaacgga ccgacggcac tgatcctctc aaggcagaat ctggcccagg
     5161 tggaacgtac gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca
     5221 gcggcggtaa gccagatatt attctgattg ccaccggttc agagatggaa attaccctgc
     5281 aagcggcaga gaaattagca ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga
     5341 ccgatatttt cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg
     5401 cggctcgcgt ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga
     5461 aaggggcaat tgtcgggatg acgggttacg gggaatctgc tccggcggat aagctgttcc
     5521 cgttctttgg ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga
     5581 aaggtgcctg atggtgatcg ccggatgctg tatgttcccc tcaccctaac cctctcccca
     5641 gaagggcgag gggacggtcc gtgcgctttt tagggccgca tctgcgattt tgtttaacgg
     5701 gtgatccaca acgtgggcct ggcgtctggc ccatgccagt tatcgcaggt aggttctgcg
     5761 gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa
     5821 tcgcttaatc cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt
     5881 gcgttcatca gttccagttc attcgtctcc tggccgttgt tgaacggcaa acgcaaccga
     5941 accgggcgcg aggctagcgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta
     6001 taggcccctg cctcacagct aatcatcatc agtgctttat catcggtcag cgcagtgaca
     6061 ttcacctcac gacgtaacgg atcaagcgag cagcgcagac cattcatccg ccagtttcca
     6121 taatccagca aatcgttacg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc
     6181 gcgacctctt tcagcgcagg cgcgggcggt acgctgagag gcggttcgtc ccctttcttg
     6241 atccacgcgg tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgccgct
     6301 tttaagccgc tcagagaaat ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc
     6361 tgaattatct gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt
     6421 gaaatccgcc acttgtcacc acttaatgcc aacggctcac catctaatag cagccgtggc
     6481 gctatctccc cttcagacgc atccggcgac ttcaacccac cgcgctcaat acgtaaaacg
     6541 gcatcggtat gcgccccagc gctgcggctc agggtcatca ccagtccatt atgatcgccc
     6601 gtattgcgcg ccacacaaaa attttgatta ttacaggtga cctgccagtc ggaaaacgcc
     6661 cgttgcgctg gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg
     6721 aaaatgcgat agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt
     6781 atcttcgttg cgcaggtagc gcggctcaat cggatttgtc ggattgagcc aataataaac
     6841 aacgaaatat tattgctatt tttcagtcca ttaaatgtga catttgcaaa tagttaagca
     6901 ataacaccgt cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga
     6961 acggcagctc gagcacttca atatcttcat cttcaacacc gccaccggcg ttagcgcgct
     7021 gattgtcact gtattcggcg ataaaaaagt ggattagctc agtcacaccg ccaggcgaca
     7081 tatacagttc aaataatttg cgcacttcgc ccacttcata gcccgtctct tcaatcgctt
     7141 ctttacgaat gcacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca
     7201 gctgcccgct ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca
     7261 cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc attaccgcga tcgtaaactt
     7321 cacgtttatg gcggataact tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca
     7381 gggtgaaata gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac
     7441 cgactccaca gcgcgaaatg aacaattata ttacgctgtg aagtcgggtt tgtctgcgca
     7501 ggctatcagt gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca
     7561 tgacgtcctt ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcgccaccg
     7621 gcgaagattt tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg
     7681 cggccccatt tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgtggattg
     7741 aagccaaacg ccataatcac cgcatcggct ggcatgacaa attcactgcc ctcaatcggt
     7801 acaggtcgcc gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatt
     7861 ccgcaaacgt gaccttgctc attcagctca agcgccaccg gctggacgtt aaattcgaag
     7921 ttggccccct cttcgcgggc gttcttcacc tctttcttcg agcctggcat gttagcttca
     7981 tcacgacgat aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag
     8041 tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa gtccggcggt attgataaac
     8101 ggctcttccg gtagctcttc gagccccatc acctgtttag tgttggcaat gaggaacggc
     8161 agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa
     8221 gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa gcaaatccaa agagacatct
     8281 ttacccactt cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg
     8341 cgtgccagca gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg
     8401 atttctggat ggcgatcgta caccgtcacc gccacgccat tgcgggtcag aacatccgca
     8461 caggccagcc ctgccggacc tgccccgata atcgccaccc gcttgtccac tttggtgaca
     8521 tgacttaagt caggacgcca acctttcgcc aacgcctgat ctgaaatata gcgttcaatg
     8581 ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg
     8641 tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg
     8701 acggcggcgt cgatgtttcc ggctttcacc agttcaatcc actgcggaat atggttatgc
     8761 agcgggcagg tccattcaca aacgctgtgc tcgccgaact taaggcagcg cgaggcttcc
     8821 cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct
     8881 tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta
     8941 ctcattaccg gcatttcttg tgcagcggta ctggcatgcc acggttgatg ctcctgacgt
     9001 gcggtgcgca agcggcggga tttcgccatg ccggagagtg cgacgtcagt gaccagttgc
     9061 agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt tttcgcgccc tgcacaaagg
     9121 tcgcatttat gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc
     9181 atcgtaccaa acggacaggc caccacgcag gatttacagc caatacactt ttgctgattg
     9241 acctgaatgc tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc
     9301 gcatcttcac aatggtgaca ggtcactgca ctacgttgct gctgatgttt gataaccgta
     9361 attcggggat gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca
     9421 caggcgattt cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga
     9481 ttcataacga ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt
     9541 acccgactta caggggaacc ggcaatgttc atgtgcccag aataagtaac tatttcgcta
     9601 taaactgtgg cagatcaaat aatcccatca ctgactaaat tgcgtttcag cgaactggaa
     9661 cattaatgat tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt
     9721 ctggcccggg ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg
     9781 ctaactctgg cgagcagttt gcgcgacgct gaggctatca atattgccgg atcgctgcgt
     9841 atgcagagtt accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat
     9901 cgccagttgt ttcagcaggc actgcattca ccggtattaa ccaacctcaa cgtctggtat
     9961 gtgccagaag cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat
    10021 aatcggctca gcaagggcga tttgccgtgg tatcaggcca atattaataa ttatgttaat
    10081 cagatagacc tgttcgtgct ggctttacag cactacgctg aacgcaaaat gctgctggtg
    10141 gtggcgattt ccctggctgg cggtatcggt attttcacgc tggtcttttt tactttgcgc
    10201 cgcatacgcc atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcagcgtatt
    10261 gaacacgggc agttcgactc gccgccgctg gataccagtc tgccgaatga gcttggtctg
    10321 cttgcaaaaa cctttaacca aatgtcgagc gagctgcata aattgtacct ttcgctggag
    10381 gcgtcagtag aagaaaagac ccgcgatctc cacgagacca agcgtcgtct ggaggtgttg
    10441 tatcagtgca gtgttcccag gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc
    10501 atattttgca gattgttcgc gacaatgaag cagctgaata tctggagctg aacgttgggg
    10561 ataactggcg gattagcgaa gggaagccaa accctgagct gccaatgcag attttaccgg
    10621 tgacaatgca agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat
    10681 cagaaccgct gcttaacagt gtttcatcga tgctgggacg cggtttgtac tttaatcagg
    10741 cgcagaagca ttttcagcag ttattgttga tggaagaacg tgcgaccatc gcccgcgaat
    10801 tgcacgactc gctggctcag gtgctttctt acttacgtat ccagttgacg ttactgaagc
    10861 gttcgatacc ggaagataac gccaccgcac aaagtatcat ggccgatttt tcccaggcgt
    10921 tgaatgatgc ttatcggcag ttacgcgagc tgttgactac tttccgcctg acgctgcagc
    10981 aggcggatct cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg
    11041 ctaaactgac cctcgactgc cgtctgccaa ccctggcact ggatgcgcaa atgcaggtgc
    11101 atttgttgca aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg
    11161 aaatcgccgt cagttgcgtc accgcgccgg acggcaatca cacggtttat atccgtgata
    11221 acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca ttatggactg aatatcatgc
    11281 gcgaacgcgc ggaacggctg ggcgggacgc tgactttttc acaaccttcc ggcggcggca
    11341 cgttagtgag tattagcttt cgctccgcag agggtgaggg gaaggggaac acgccggctg
    11401 aggtgtaaat aattaccctc acaccggccc tctcccttta aaggcgagag ggaaaaacgt
    11461 gcccatattg acctgtaagc gagtgtgtca gcaatctcat gataatttac attaactcct
    11521 ttttttctcc acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac
    11581 aatacgcaga aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt
    11641 ttgcctgggt gctgacaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc
    11701 ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg
    11761 gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg
    11821 gcctcgataa tctgatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca
    11881 ctttaagttt taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc
    11941 tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta
    12001 aaaccggcga taccaacatt ctgaccattg ccttcgtctc taccgatggc tcgatggata
    12061 aacaggatat tgctgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg
    12121 gcgtcgggga tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg
    12181 cgaaactcaa cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga
    12241 acgcgcagat tgcggttggg caacttggtg gtacaccttc cgtcgataag caggcgctca
    12301 acgccaccat taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct
    12361 tgcgggtgaa tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg
    12421 gggcggagaa atacgattat cttagccgct tcaatggtaa gccagcctcc gggttggggg
    12481 taaaactggc ctccagcgca aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg
    12541 acgagctggc gcagtatttc ccgcacggac tggaatacaa ggtggcgtat gaaaccacct
    12601 cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg
    12661 ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg
    12721 ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca
    12781 acaccttaac catgttcgcg atggtgctgg cgatcggtct gctggtggat gacgccatcg
    12841 tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca
    12901 cacgtaaatc gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg
    12961 cggtatttgt accaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct
    13021 ctattaccat tgttgcggcg atggtgctgt cagtactggt agcgatgctc ctcactccgg
    13081 ctctgtgtgc cacgctactt aagccactga aaaaaggtga gcatcacggg caaaaaggtt
    13141 tttttgcctg gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg
    13201 cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca
    13261 tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cggggcatgt
    13321 ttactacctc ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg
    13381 agcaaatcga gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca
    13441 ccgttggttc tggccctggg ggtaacgggc aaaacgtggc gagaatgttt atccgcctga
    13501 aagactggag cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa
    13561 cgaaggcgtt taaccaaatt aaagaagctc gcgttatcgc cagcagccca ccagcaatta
    13621 gcggtcttgg tagttctgca ggttttgata tggagttgca ggaccacgct ggagcgggtc
    13681 acgatgcgct gatggcagca cgtaatcagt tgctggcgct ggcggcggaa aacccggagc
    13741 taacccgtgt gcgccataac ggcctcgatg acagtccgca gttgcagatt gatatcgacc
    13801 agcgtaaagc tcaggcgctg ggcgttgata tcgacgatat taacgacaca ctgcaaaccg
    13861 cctggggttc gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg
    13921 tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa
    13981 ataaagatgg cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gaaacaggct
    14041 cgccgcgtct ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac
    14101 cgggggtcag taccggtacg gcgatggata ttatggaatc gttagtgaag cagctgccaa
    14161 acggctttgg tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg
    14221 ctccggcgct gtacgccatt tccttgctgg tggtattcct gtgtctggcg gcattgtatg
    14281 aaagctggtc ggtaccgttc tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc
    14341 tgctggcaac ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa
    14401 cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga
    14461 accaaaaagg ccatgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc
    14521 cgattctgat gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg
    14581 gtgccggttc cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcag
    14641 ctactattct ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc
    14701 cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac atgccaatgt gtcgcctttt
    14761 tcaactttcc gattaagaac ctgctcagcg ggttcttgct gtttatactt tgtctcagga
    14821 attacttacg aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca
    14881 taacaacctc tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa
    14941 tttactcgat tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc
    15001 ctgcggtgat aatttgtatg tgatacacag caacatttcg agatattcat acggcatcta
    15061 a
//
LOCUS       DAZPDE010000095        14481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPDE010000095 DAZPDE010000000
VERSION     DAZPDE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.095"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHU0J_002602"
     CDS             <1..118
                     /locus_tag="ACHU0J_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217243.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHU0J_002603"
     CDS             complement(115..381)
                     /locus_tag="ACHU0J_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1217244.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHU0J_002604"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHU0J_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1217245.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="ACHU0J_002605"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="ACHU0J_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="ACHU0J_002606"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="ACHU0J_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1217246.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHU0J_002607"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHU0J_002607"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1217247.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHU0J_002608"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHU0J_002608"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1217248.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHU0J_002609"
     CDS             complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHU0J_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1217249.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHU0J_002610"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHU0J_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1217250.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHU0J_002611"
     CDS             complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHU0J_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1217251.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHU0J_002612"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHU0J_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHU0J_002613"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHU0J_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="ACHU0J_002614"
     CDS             complement(12643..14097)
                     /locus_tag="ACHU0J_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR1217252.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="ACHU0J_002615"
     CDS             complement(14094..>14481)
                     /locus_tag="ACHU0J_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1217253.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3418 g         3551 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAZPDE010000096        14438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPDE010000096 DAZPDE010000000
VERSION     DAZPDE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.096"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHU0J_002616"
     CDS             <1..187
                     /locus_tag="ACHU0J_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1217254.1"
                     /translation="YEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHR
                     VDIGNLHYIDTGAVFGG"
     gene            complement(212..403)
                     /locus_tag="ACHU0J_002617"
     CDS             complement(212..403)
                     /locus_tag="ACHU0J_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1217255.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(508..744)
                     /locus_tag="ACHU0J_002618"
     CDS             complement(508..744)
                     /locus_tag="ACHU0J_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR1217256.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(862..2301)
                     /gene="rsmF"
                     /locus_tag="ACHU0J_002619"
                     /pseudo
     CDS             complement(862..2301)
                     /gene="rsmF"
                     /locus_tag="ACHU0J_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2381..5001)
                     /gene="yebT"
                     /locus_tag="ACHU0J_002620"
                     /pseudo
     CDS             complement(2381..5001)
                     /gene="yebT"
                     /locus_tag="ACHU0J_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4970..6253)
                     /gene="yebS"
                     /locus_tag="ACHU0J_002621"
     CDS             complement(4970..6253)
                     /gene="yebS"
                     /locus_tag="ACHU0J_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1217257.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6383..6880
                     /gene="msrC"
                     /locus_tag="ACHU0J_002622"
     CDS             6383..6880
                     /gene="msrC"
                     /locus_tag="ACHU0J_002622"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1217258.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6977..7675
                     /gene="proQ"
                     /locus_tag="ACHU0J_002623"
     CDS             6977..7675
                     /gene="proQ"
                     /locus_tag="ACHU0J_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1217259.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7695..9743
                     /gene="prc"
                     /locus_tag="ACHU0J_002624"
     CDS             7695..9743
                     /gene="prc"
                     /locus_tag="ACHU0J_002624"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1217260.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9935..10816
                     /gene="htpX"
                     /locus_tag="ACHU0J_002625"
     CDS             9935..10816
                     /gene="htpX"
                     /locus_tag="ACHU0J_002625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1217261.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10862..12235)
                     /locus_tag="ACHU0J_002626"
     CDS             complement(10862..12235)
                     /locus_tag="ACHU0J_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217262.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12412..13203
                     /gene="kdgR"
                     /locus_tag="ACHU0J_002627"
     CDS             12412..13203
                     /gene="kdgR"
                     /locus_tag="ACHU0J_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1217263.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13346..13585)
                     /locus_tag="ACHU0J_002628"
     CDS             complement(13346..13585)
                     /locus_tag="ACHU0J_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1217264.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13744..13887
                     /gene="mgrB"
                     /locus_tag="ACHU0J_002629"
     CDS             13744..13887
                     /gene="mgrB"
                     /locus_tag="ACHU0J_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1217265.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13962..14249
                     /locus_tag="ACHU0J_002630"
     CDS             13962..14249
                     /locus_tag="ACHU0J_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1217266.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3792 a         3521 c         3698 g         3427 t
ORIGIN      
        1 ttatgaatgg caaaaggacg ttgatttgca tcaggtcttg tggagccgct cgcgattagg
       61 tgaacgccca aaagggcagg gaattacagg tgctgatcat ttctggtttg gtcatacacc
      121 gttgcgacat cgcgtggata ttggcaacct gcattatatt gataccggtg ctgtctttgg
      181 gggctaactg actcttgtgc aactgcaata attaaaaatc accatactcc tgtgctggtt
      241 gccagaaacc atctataaaa tcctcaatcg gaaaacaact gccatggcgg atccgttgat
      301 cgctcataga ataaagacac tgctgttccg tgttgtagac atccacaaca atatcttcac
      361 aaccgccatc caggtagcaa acaaaaagta ccagcgcgaa catttcatcc ccgaagtgtg
      421 gtgccgtacc gttaagttta ggagagattt tacaacgggg gaataaccag gacaaataac
      481 ccgccatgcc gacgggttct ttttggatca ggcaagacgc ataatccagg catcagattg
      541 ttgatcgtaa cgggtacggt aaaggacaga gtgttcgcca tttagcaggg cagggatact
      601 ggtttcagca tcccaggcat ccagttgcat acataaatcg gggtcagatt tacaaggaat
      661 ggttaacgtt cgatcacccg gcgattcacc gtgtaattcg ccgtcgtcga tttcaaaagc
      721 gcctatgcgc acactggttt tcatcttcgt gctctccatt tagctgctga ttgtagtacg
      781 accagtccga tcgcacaatt tttcagccta gttgaggccg gcgaatgtgc cagtcaaaaa
      841 gtgcgctttt tttgtgcgct gtcatgcgtt actggtaaaa agtttcccgt cccgcaccag
      901 ttcacgcggg tagctgtttt tcagtcgcga accaatccgt ttggctaaac caatcggctg
      961 atgctggaaa gtaaccaaca cgtcatccgc cactggcgcg gcttgcgggt aaacatcgcg
     1021 cccgcgatac cactcctacg cttcctgcgg tgtcagttca aaagcgttta cattgtcggg
     1081 ggaggcatgg gcaataaccg cttcatgctg ccagcgataa cctttgttat gcgtttcggc
     1141 aagtttaatc cctaagcgag aaaatcggac tttaccgatc agggcttcaa tgcctaccgg
     1201 gaacaaccac agttctttgt cgcgttgcca gagtcgcagg tttccatccc agtttaagcc
     1261 aacacttgca gccgcctgac gaatttgtcc ggcttcgcga tctttcaccg ggctgaacgg
     1321 gaaattgccc actttgtatt ttggggcggg taaggcgggg atcgcctgag ttttacgcag
     1381 acgagcaacg aagaagcctt cgcagtcgta aatttgtggg aaaacatgca aaaaaccttc
     1441 ttcggtcagc gccttgtttg caccagggaa gagatcgcca agtggtaaaa actctacttc
     1501 gtcggggtaa gtctctttca gccaccggca aacggcttcg ttttcttccc ggtttaaggt
     1561 ataggtcgag taaaccagcg taccgccagg acgtaatgca tgaaaggcgc tgtcaatcag
     1621 ttcccgttgg gtagctgcga tttcctgatt gctttcgggt gaccagtttt ttagcgcatc
     1681 gggatcttta cgcaccacgc cttcgccgga gcagggagcg tccagcaaaa tggcatcaaa
     1741 catttctggc actgccgcac caaacacgcg gccaccaaaa tgtgtgagcg caacattact
     1801 gatgccacag cggctgatat tggcatgtaa cacttttacc caactggcgg aaaactcatt
     1861 ggcaaggatt gccccttcgt tattcatccg cgcggcaatt tgcgtcgttt ttgagcctgg
     1921 cgcagcagcg acatccatca cccgctgtgg tgcattaccg tcagcaaaca aggcggcgac
     1981 gggcaacatt gaactggctt cctgaatata aaacaggcca cttaaatgct cggtggtact
     2041 acccaatggc aatgcatctt cattgtcgcg ttcaatccag aaaccttctt cacaccacgg
     2101 aattggcgta agcgtccagc cataaggagc ggttaattgc aggaaatcag caacggagat
     2161 tttcagcgta ttaacgcgaa tgctgcggcg caacgggcgc tgacaggcgg caagaaaatc
     2221 atcaaatgag agcgtcgaag gcatagcttc gcgcatttgt gtcagaaacg cgtccgggaa
     2281 ataaacggtg tgttgggcca cgagcatgta ccactggcaa aaaaacaggc gcgcagttta
     2341 gcataagcgc tccggcgcag gcacgccgga gcagtgggca ttatttggga agcgcagttc
     2401 cccattcacg ccactctttc ggttcacttt cctgcaacag gaagtgtttg ccttcttggg
     2461 ctttcggtgc aagtggcgta cccggaggcg tggcgaaggc gatgccgcca cggataaatt
     2521 ggttaaaggt gccggttttc actacgccgc ccgtcagacc aaagtccaga ctgtaacccg
     2581 atgccaacca gaagacggaa ttgttacgca ccaggtgttg atagcgttta ctgatgcgca
     2641 tcgcaatcat cacgcgatct gacaatgtcc ccagcgtcat tccggtaacc gtacctactt
     2701 ccagaccacg gaacagcaca ggcgtaccga tgcctaacga accggcttcc ggcgcttcaa
     2761 caataatgct taaaccatct aggtaacgcg aatcagtaat ggtggcctct tctaattcaa
     2821 agtcgcgacg aggattgcca cggcctggtt cgacgttgat atacggctgg aggatagtat
     2881 ctagatgctc aacgccagct gccgagattt gcggtgtgac cactgagaag cgcgtaccac
     2941 cgcgagcaaa ggtctggaca tattccggat agagcaccgc ctttgcctgt acttcattgc
     3001 gtgcggtaat cagatccagc gtctggattt gcccgatatc aataccgaga tagcgaattg
     3061 gcatcccgac cgccagtttt ccggcatcga aagcgtgaag cgtaatctgc ctgccaaccg
     3121 cacgggccgc tgtttcggaa gcatacagaa ttcgcttgtc gcctttacgc tgactggcgc
     3181 tggcaccact gaggttatcg aagctaatgg ccccctttaa tgctctggag agcggggatg
     3241 cctgtacggt caggccacta ccattcagct gaactttcgc cccgccttct gcccagaaca
     3301 cgctgttgct ggtcaaaagg ttgcgatatt ccggtttaat gtgcagatcg atatcgaacg
     3361 cgttagctcg cggacgcacg gtaataactt caccaacttc aaatttacgg tacagcacta
     3421 ccgatcctgc ctgcacatcc ggcagcgtct ctgcactcaa actcatggtt gtggtgggta
     3481 aatcgctaag gctgttctcc agcgcttttt ccagattggc atacagtgga tagctggctt
     3541 tcatctcgcc tttatcgccc ggcagaatac gtatcccgcc gttaatccat tctgaggcgc
     3601 tggcaccgag aaactcaacg ccatccagcc ccaccttcac gtcgacacgg ctgttgacga
     3661 caaatttgct atcgcctttt accagttctc gatgctgagg ctcgatggcg acggtaaagg
     3721 tgacgccttt gctggtgagt ttacgatcga taacctggcc tacctgcacg ccgtgaagaa
     3781 tgagcggctg acccgcatca ataccgtaac tttccggtgc tgtcagggtc agtgtcagaa
     3841 cgtccggctc ttgcagcagg gctttttcgc ccggcacaac aacgaactct ttgcgtggct
     3901 cgccatcgcc gggcaccaac tcaaaggttt ttccggtcag cagggcgctg agattagcat
     3961 cgctaaggga taatttcggg ttgcgtaatt cgatgcgggt attttcacga agcagggatc
     4021 aacggtcatt tccccagtga ctttaccacc aggatttaaa tccagtttag tcagctgtcc
     4081 gacttccagc ccctgataca ttaacggcgt cgagtcggcg gttaatcctg ctccactcgg
     4141 cagttccagt tttattatta ctccacgctg gctgtgggcc agatcttcat acagaccaaa
     4201 ggtatcttcc gcctcggcag gtttcgactc ttcaggtgaa tcgaaggcaa tcgcaccgtt
     4261 aaccagtgcc gccagacttt ccagtttcac cttcgcgcca ctgatactga cgttggcatc
     4321 aacgccggaa acgtttcaga aacggctacc ttttttcacc agatcggtaa aacgccgctc
     4381 gatcaggaca tcaatcacca cgccttgctt gttgggattg atggcatagt cgtagacttt
     4441 tcccaccggg atcttgcgga aatagaccaa tgaaccgctg ttcagcgaac cgagagcggg
     4501 ggcttgcagg tggatcatca gatcgccatt gtccagccga tatttcggtt gggtatcgag
     4561 cgcgacaaag tgatcctgct ctttaccttt acccggcatc atgccgatat agttcccacc
     4621 gacgagggcg tccagcccgg agacacctgc caacgatgct tttggcgtca tcagccagaa
     4681 ctgagtctct tcgcgcagcg catctttcat atcggacttg atgctgacct tgacttcaat
     4741 cttacgaaga tcgtcgctga ggctgatatc ctgcactgtt ccgacttcaa cgccctgata
     4801 acgaacaggc gtacggcccg gaacaatacc atccgccgac ataaagtcga tggtgacggt
     4861 attaccccgg tcctgataac tgtcccaaat cagccaaccg gcaatcatta gcgcgatgaa
     4921 aggcagcagc cagaaaggtg agatacggcg tttattttta atctgcgctt cagtcgtcga
     4981 agcgggcgtt tcctgactca tgtgcatccc aaagtaagcg gctgtccagc cattccacag
     5041 caagaatagt caaaattacg gcagcgccaa aataaaacgc agccggtccc atagtaaaag
     5101 cgaggatctg atcgcgatta atcagcgaca tggttaaaga tatgacaaac aggtcgagta
     5161 tcgaccagcg tccaatccag gtcaccatcc gcagtaacag aatgcgtgtg cgtaaacctt
     5221 gctggcattt aaaatgaatg ctcaacagta aagtgaacat gacgatcact ttagtaaacg
     5281 gtaccagaat actggcgata aacacgattc ccgcaaccgc aatgttgcta cttgccagcg
     5341 acataattcc ggaaagaatc gtatcttcct gccggccacc attcagataa atgatagaaa
     5401 taggcaatag gttggctggt aacaacaaaa cgattgacgc taacagcgcc gcccagcatt
     5461 tttgcaaact atgacgacga cgcaggcgta gcgggatatg gcaacgcggg cagagaccac
     5521 gctgatcagg atagccggta aaatggcacc caagacagac acgaagtttc tcgtccctac
     5581 gcgtagcggg gcgctgcgga taatatcgct cccacagctc ctcaacatta agatgtgaca
     5641 acgtcaccgt cgttaaaatc accaacgcca caaaagagaa caagccgaca cccgcctgga
     5701 tatgggcata atcctgtacc tttatagaag caacgccaat gccgaccagg tagatatcca
     5761 gcattaccca ctctttaagt cgctcaagca tcagcagtac cggacgtaaa ttcattccca
     5821 gtcggttacc aaaccataaa tatgctatgg aggacaccag aatgaggggg gcaccaataa
     5881 cgcagaaaaa gaccattgat cccgttatcg catcgccctg tttggtcatt tgccagatgc
     5941 cttgcatgac gttggcgtca atacgaatgc ctaacagcca gatatgcaac agcggttcgc
     6001 cccaggcaaa cggcataagc aacagcatgg tgaaggccat tgccgccagg cgcgttagcg
     6061 accagtcgcg cccgtcgcga atttttgcct gacagcgcgg acaataggca ctttgatgag
     6121 aatttatctc gggcaggcta aacagcatgt cacattgcgg gcaacgttgg taatcaccac
     6181 gcggcagttc ctcgccgatt gccctcactg ttatcttttt tgtcggcgta atttgtggtg
     6241 tgttaagagc catgacgtgc caaatgtaat aagaattaac attaatctta acccatgatg
     6301 ggaatgatct tgagcattaa cgcatttaat gcttatttta ataggctggc agttccaggt
     6361 aagacaacca agtgattaag taatgaacaa aacagaattt tacgcggatt taaatcgcga
     6421 ctttaacgcg ctgatggcgg gagaaaccag ttttctggca acgcttgcga acaccagtgc
     6481 gttgttatac gagcgtctca ctgacgttaa ctgggcaggt ttttatttgc ttgaggacga
     6541 tacactggta ctcggaccat ttcagggcaa aattgcctgt gtccggatac ccgtcgggcg
     6601 cggggtgtgc ggcacagcgg ttgcccgcaa tcaagtgcag cgtatcgagg atgttcacgc
     6661 gtttgacggg catattgcct gtgatgcggc gagtaattct gaaattgttc tgccgctggt
     6721 ggtgaaaaat cagattattg gtgttctcga catcgatagt actgtcttcg ctcgctttac
     6781 agacgaggac gagcaaggct tacgtcagct tgtggcacag cttgaaaaag tgcttgcaac
     6841 gacggattac aaaaaattct ttgcgagcgt cgcaggataa tcaacggata acgtagcatt
     6901 tactggtagc gtcattataa tgacgcctgt tcatgcctgc gcttgttggc tacgtccgtt
     6961 gtaatcagga aatttcatgg aaaatcaacc taagttgaat agcagtaaag aagtaatcgc
     7021 gtttctggcc gaacgttttc cccactgttt cagtgcggaa ggtgaagcgc gtccgctgaa
     7081 aattggtatt tttcaggatt tggtcgatcg tgttgccggg gaaatgaacc tgagcaaaac
     7141 gcaattacga tccgctttac gtctctacac ttcgagctgg cgttatcttt acggtgttaa
     7201 acccggcgca acgcgtgtcg atcttgacgg caacccatgc ggtgagctgg acgagcaaca
     7261 tgtagagcat gctcgcaagc agcttgaaga agcgaaagcg cgtgttcagg cacagcgtgc
     7321 tgaacagcaa gcgaaaaaac gcgaagctgc cgcagctgct ggtgagaaag aagacgcacc
     7381 gcgccgcgaa cgcaagccac gtccgaccac gccacgccgc aaagaaggcg ctgaacgtaa
     7441 acctcgtgcg caaaagccgg tagagaaagc gccaaaaaca gtaaaagcac ctcgcgaaga
     7501 acagcacacc ccggtttctg acatttcagc tctgactgtc ggacaagccc tgaaggtgaa
     7561 agcgggtcaa aacgcgatgg atgccaccgt attagaaatc accaaagacg gcgtccgcgt
     7621 ccagctgaat tcgggtatgt ctttgattgt gcgcgcagaa cacctggtgt tctgaaacgg
     7681 aggccgggcc aggcatgaac atgtttttta ggcttaccgc gttagctggc ctgcttgcaa
     7741 tagcaggcca gaccttcgct gtagaagata tcacgcgtgc tgatcaaatt ccggtattaa
     7801 aggaagagac gcagcatgcg acggtaagtg agcgcgtaac gtcgcgcttc acccgttctc
     7861 attatcgcca gttcgacctc gatcaggcat tttcggccaa aatctttgac cgctacctga
     7921 atctgctcga ttacagccac aatgtgctgc tggcaagcga tgttgaacag ttcgcgaaaa
     7981 agaaaaccga gttaggcgat gaactgcgtt caggcaaact cgacgttttc tacgatctct
     8041 acaatctggc gcaaaagcgc cgttttgaac gttaccagta cgctttgtcg gtactggaaa
     8101 agccgatgga tttcaccggc aacgacactt ataaccttga ccgcagcaaa gcgccctggc
     8161 cgaaaaacga ggctgagttg aacgcgcagt gggacagtaa agtcaaattc gacgagttaa
     8221 gcctgaagct gacaggaaaa acggataaag aaattcgtga aaccctgaca cgccgctaca
     8281 aatttgccat tcgtcgtctg gcgcaaacca acagcgaaga tgttttctcg ctggcaatga
     8341 cggcgtttgc gcgtgaaatc gatccgcata ccaactatct ttccccgcgt aataccgaac
     8401 agttcaacac tgaaatgagt ttgtcactgg aaggtattgg cgcagtgctg caaatggatg
     8461 atgactacac cgttatcaat tcgatggtgg caggtggtcc ggcagcgaag agtaaagcta
     8521 tcagcgttgg tgacaaaatt gtcggtgttg gtcaaacagg caagccgatg gttgacgtga
     8581 ttggctggcg tcttgatgat gtggttgcct taattaaagg gccgaagggc agtaaagttc
     8641 gtctggaaat tttacctgct ggtaaaggga ccaagacccg tactgtaacg ttgacccgtg
     8701 aacgtattcg tctcgaagac cgcgcggtta aaatgtcggt gaagaccgtc ggtaaagaga
     8761 aagtcggcgt gctggatatt ccgggcttct atgtgggttt gacagacgat gtcaaagttc
     8821 aactgcagaa actggaaaaa cagaatgtca gcagtgtgat catcgacctg cgtagcaatg
     8881 gcggtggggc gttgaccgaa gcggtatcgc tctcaggtct gtttattcct gcgggtccca
     8941 ttgttcaggt ccgcgataac aacggcaagg ttcgtgaaga cagcgatacc gacgggcagg
     9001 tgttctataa aggcccgctg gtggtactgg ttgaccgttt cagtgcttcg gcttcagaaa
     9061 tctttgccgc ggcaatgcag gattacggtc gtgcgctggt tgtgggtgaa ccgacgtttg
     9121 gtaaaggcac cgttcagcaa taccgttcat tgaaccgtat ttacgatcag atgttacgtc
     9181 ctgaatggcc agcgctgggt tctgtgcagt acacgatcca gaaattctat cgcgttaacg
     9241 gcggcagtac gcaacgtaaa ggcgtaacac cagacatcat catgccgacg ggtaatgaag
     9301 aaacggaaac gggtgagaaa ttcgaagata acgcgctgcc gtgggatagc atcgatgccg
     9361 cgacttatgt gaaatcagga gatttaactg cctttgaacc ggagctgctg aaggaacata
     9421 atgcgcgtat cgcgaaagat cctgagttcc agaacatcat gaaggatatc gcgcgcttca
     9481 acgctatgaa ggacaagcgc aatatcgttt ctctgaacta cgctgtgcgt gagaaagaga
     9541 ataatgaaga tgatgcgacg cgtctggcgc gtttgaacga acgctttaaa cgagaaggta
     9601 aaccggagtt gaagaagctg gatgatctat cgaaagatta ccaggagccg gatccttatc
     9661 tggatgagac ggtgaatatc gcactcgatc tggcgaagct tgaaaaagcc agacccgcgg
     9721 aacaacccgc tcccgtcaag taatatcaat caggcacaag aaattgtgcc tgatttttta
     9781 acagcggcaa gatgccgtaa atcagatgct acaaaatgta aagttgtgtc tttctggtga
     9841 cttacgcact atccagactt gaaaatagtc gcgtaaccca tacgatgtgg gtatcgcata
     9901 ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg cgaatcgcgc tcttcctgct
     9961 aacgaacctg gccgtaatgg tcgttttcgg gctggtactg agcctgacag ggatacagtc
    10021 gagcagcgtt caggggctga tgatgatggc cttgctgttc ggttttggtg gttccttcgt
    10081 ttcgcttctg atgtccaaat ggatggcatt acgatctgtt ggcggggaag tgatcgagca
    10141 accgcgtaac gaaagggaac gttggctggt caatactgta gcaacccagg ctcgtcaggc
    10201 ggggatcgct atgccgcaag tggctatcta ccatgcgccg gacatcaacg cttttgcaac
    10261 cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc ggtttgctgc agaacatgag
    10321 cccggatgaa gccgaggcgg taattgctca cgaaatcagc cacatcgcca atggtgatat
    10381 ggtcaccatg acgctgattc agggcgtggt gaacaccttc gttatcttta tttcccgtat
    10441 tctggcgcag cttgccgcgg gttttatggg cggaaatcgt gatgaaggtg aagagagcaa
    10501 cggcaacccg ctgatctact ttgcggttgc aacggttctg gaactggtgt ttggtattct
    10561 ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa ttccatgcag atgccggttc
    10621 ggcaaaactg gttggtcgcg agaaaatgat tgccgcgctg cagcgcctga aaaccagcta
    10681 tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt aacggtaagt cgaaatcgct
    10741 cagtgagttg ttcatgactc acccgccgct ggataaacgt attgaagctc tgcgtacggg
    10801 tgaatacctg aagtaatatg aagtaaatac taaaaagcgc gtcctgatcg acgcgctttt
    10861 tttatgccct ggctcgtggc tgagtgatac gtaaaccact gacacaggca gcaatcactg
    10921 ccagaatagc cgcagccatc agcgagacgt gcgtaccatt atcaccaaac tgatttagca
    10981 tcagcgccac cagcgccgcg ccactactct gacccagtag acgagccgtt cctaacatgc
    11041 cactggcccc accgctacgt tcgcgcggag cggaggtaat aatggtgtgg ttattgggtg
    11101 actggaataa gccaaatcca gcgccacata agatcatcgg ccagataata ttgatatccg
    11161 caggtgacgc gggcagcaga accagggaaa aaagccccgt agccatgatg aacaacccta
    11221 aagcacccag caatcctgca tgtacgcgtt caatcaaata gcctgccaga ggagccatca
    11281 ccatcgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga
    11341 gcacggtttg caggtaaaag ggcagggaaa ccattgccag catttgtgcg cagaaagagc
    11401 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca
    11461 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacaaca accattacca
    11521 ccagttccgc agcaattaac gtcagcgatt gcccctgagc gaaaccactc aacgcagtga
    11581 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
    11641 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccta
    11701 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
    11761 ttgtcggccc ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctcc
    11821 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
    11881 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
    11941 cgcagaacag tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc
    12001 caaacatatc gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg
    12061 cgttcactac ccagatggaa ctggctggtg tggcatgaag gtccgtggca attgttggca
    12121 gggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta
    12181 atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa
    12241 ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg
    12301 atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt
    12361 ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac
    12421 gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg
    12481 caggcgctgg gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg cgtcatgatg
    12541 tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag
    12601 gaaggggagt cggagaaata ttccctgacc ctgaaattgt ttgaactggg cgctcgcgcg
    12661 ttacaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat ctcccgcctg
    12721 accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa
    12781 attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac
    12841 agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gaagcaaatt
    12901 cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta
    12961 ttacccgttc tggaccaggt gcgcgagcag gggtatggag aagataacga agagcaggaa
    13021 gaagggctgc aatgcattgc ggtaccggta tttgatcgct ttggcgtggt cattgccggt
    13081 ttgagcatct ccttcccgac gttgcgtttc tctgaagagc gtttacagga atatgtcgca
    13141 atgttgcata ccgcagcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc
    13201 tgatgagagt aagaacctgt cggaatatca aacagacagg ttctttattt agcatgagaa
    13261 aaataaagtt gaaggtgcgt tatattaaac gcgcttgcta taagaatatt ttactcatga
    13321 gtgagaatct ggttatttat tacccttaac cattatcgac cacgatattg cttttacgta
    13381 acagtgggca atctgttatc tccaagaaac cacttttagt gtgcaagtat tgtaccgtgc
    13441 tggtgcctct ggcagtcaga taggtacatt gcaaacctaa tcctgcggca ttctctttgc
    13501 ttccaatcaa aacgccatat ccgctgagta ataatcctat ccataccagt gctatcagca
    13561 taactgtgcg aatgatgaat cgcattacaa tctcttctct ttttatgttc gcttaatcgt
    13621 agcggcaata tgcgctgaag caagctactc attccgaaaa agcacgaata tcgacatagt
    13681 taggcgctgt ttaactaacg catgctagtt taatgacata aggtaggtga agcggagatt
    13741 ggagtgaaaa agtttcgatg ggtcgttctg gttgtcgtgg tgttggcttg cttgctgctt
    13801 tgggcgcagg tattcaacat gatgtgcgat caggatgtac aatttttcag cggaatttgt
    13861 gccattaacc agtttatccc gtggtgatat cattttttca gcactagatt ttcttcctct
    13921 ccagcagtgg tagaatagcg cctctactca gaggtggtga aatgaacgaa gttgtaaatt
    13981 caggcgtgat gaacattgcg tctttggttg tatcggtggt ggttcttctt atcgggctca
    14041 tcttgtggtt ttttatcaat cgtgccagtt cacggactaa cgaacagatt gaactgcttg
    14101 aggcgttgct ggatcagcaa aaacgtcaaa atgcactgtt acgtcgtttg tgcgaagcaa
    14161 acgaaccaga gaaagcagat aaaaagacca ttgagagtca aaaatcggtt gatgacgaag
    14221 atattattcg cctggtcgcc gaacgataat ccttcctcaa tcttgagtaa ccccgtcgtt
    14281 gaagggttac tcaattcatc ctgattatac gcactgtcat ggtgcaactg cttcaaatta
    14341 aaactctggt ctgacttcaa ataattatca tattatttat ttgcgtaacg ggatatcaga
    14401 aaaagatatt ttcctcatta atttattagg atgtttac
//
LOCUS       DAZPDE010000097        14391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPDE010000097 DAZPDE010000000
VERSION     DAZPDE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.097"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            144..710
                     /locus_tag="ACHU0J_002631"
     CDS             144..710
                     /locus_tag="ACHU0J_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1217267.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            761..3220
                     /locus_tag="ACHU0J_002632"
     CDS             761..3220
                     /locus_tag="ACHU0J_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1217268.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3235..3966
                     /locus_tag="ACHU0J_002633"
     CDS             3235..3966
                     /locus_tag="ACHU0J_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1217269.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3963..5039
                     /locus_tag="ACHU0J_002634"
                     /pseudo
     CDS             3963..5039
                     /locus_tag="ACHU0J_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5191..6237
                     /locus_tag="ACHU0J_002635"
     CDS             5191..6237
                     /locus_tag="ACHU0J_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1217270.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6234..7025)
                     /gene="nei"
                     /locus_tag="ACHU0J_002636"
     CDS             complement(6234..7025)
                     /gene="nei"
                     /locus_tag="ACHU0J_002636"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1217271.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7061..7795)
                     /gene="pxpA"
                     /locus_tag="ACHU0J_002637"
     CDS             complement(7061..7795)
                     /gene="pxpA"
                     /locus_tag="ACHU0J_002637"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1217272.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7785..8717)
                     /gene="pxpC"
                     /locus_tag="ACHU0J_002638"
                     /pseudo
     CDS             complement(7785..8717)
                     /gene="pxpC"
                     /locus_tag="ACHU0J_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8711..9367)
                     /gene="pxpB"
                     /locus_tag="ACHU0J_002639"
     CDS             complement(8711..9367)
                     /gene="pxpB"
                     /locus_tag="ACHU0J_002639"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1217273.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9390..10133)
                     /gene="ybgI"
                     /locus_tag="ACHU0J_002640"
     CDS             complement(9390..10133)
                     /gene="ybgI"
                     /locus_tag="ACHU0J_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1217274.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10404..11885
                     /gene="dtpD"
                     /locus_tag="ACHU0J_002641"
     CDS             10404..11885
                     /gene="dtpD"
                     /locus_tag="ACHU0J_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1217275.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(12035..13453)
                     /gene="phrB"
                     /locus_tag="ACHU0J_002642"
     CDS             complement(12035..13453)
                     /gene="phrB"
                     /locus_tag="ACHU0J_002642"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1217276.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13450..13959)
                     /locus_tag="ACHU0J_002643"
     CDS             complement(13450..13959)
                     /locus_tag="ACHU0J_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1217277.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            14088..14375
                     /locus_tag="ACHU0J_002644"
     CDS             14088..14375
                     /locus_tag="ACHU0J_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR1217278.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
BASE COUNT         3514 a         3848 c         3504 g         3525 t
ORIGIN      
        1 acatttaatt cataccatca attaattact tttacattta ataaaattaa taatattttt
       61 aagaaaaact aaaaacattc gacaatcaaa taagatattc ttgcaccgat atactttttt
      121 cttttctgtt aaaggactca gttatgttta aaggacaaaa aacattggcc gcactggccg
      181 tatctctgct gttcacagca cctgtttatg cggctgatga aggatccggt gaaattcact
      241 ttaaaggtga agttattgaa gcaccgtgtg aaatacatca ggatgatatt gataaagagg
      301 ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc gatgccgttg
      361 ctgtcgacct gctcttagtc aactgtgatc tggaaaactc cagcaacggt tccggtggca
      421 agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc gcagatccga
      481 ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag
      541 atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct ccgacctcca
      601 gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct acacctgtaa
      661 cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa gtattacttt
      721 ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg tgaatattta
      781 tcgactctct tttgtatcct gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt
      841 caatctgaat gttctcgata aatcaatgcg cgaccgcatt gatatttcat tattaaagga
      901 aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata ataaccaaat
      961 cagtaacgag caaaagatta actggcacaa aaatgacgat aaaaccattc cgtgcatcaa
     1021 tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt taccattgat
     1081 aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa
     1141 tcagcaactg aatattagta ttccgcaagc ctggctggcg tggcactcag aaaactggac
     1201 cccaccctcc acatggaaag aaggtgtcgc cggtgtcctg atggattaca acttgtttgc
     1261 cagcagctac cgcccacagg atggcagcag cagcactaat cttaacgcct atggaacagc
     1321 cggaatcaac acgggggcat ggcgcttacg cagtgattat cagttgaatc atactgatag
     1381 cgatgataac catgaacagt caggcgaaat atcacgtacc tatctttttc gcccgttacc
     1441 gcaattaggt tcaaaattaa ccctcggcga aacggatttt agtcccaata ttttcgacgg
     1501 tttttcttat accggcgcgg cactggcaag tgacgatcga atgttgccat gggaactacg
     1561 cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga cgatcagtca
     1621 atcaggccgc gtcatttacc agaaaaaaat cccaccaggc ccatttatca ttgacgacct
     1681 taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg gtcgggtgaa
     1741 caatttccag gtttcagcag catcgacgcc cttcctgact cgtcagggac aggttcgcta
     1801 taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa atgaaacctt
     1861 ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtaca gcggcctgct
     1921 gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata tgctgtggct
     1981 tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc agcaggacga
     2041 gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta acagcacgat
     2101 ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca actatctgga
     2161 tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct tatctgtggg
     2221 ccaaccgatt accccactaa acctcaatct ttacgccaac ctgctacatc aaacctggtg
     2281 gaatgcagac gcctccacga ccgccaacat cacggcaggt tttaatgttg atattggtga
     2341 ctggagagat atctctattt ctacgtcatt caatacaacc cattacgaag ataaagatcg
     2401 cgacaaccag atttacctgt cgatttcgct ccccttcggt aacggtggtc gggttggcta
     2461 tgacatgcaa aacagtagcc acagcaccac acaccgcatg tcgtggaacg atacgctgga
     2521 tgaacgtaat agctggggca tgtctgccgg actgcaatcc gaccgtcctg acaatggagc
     2581 ccaggtgagc ggtaactatc agcacctgag ttcagcgggt gagtgggata tttctggtac
     2641 ctatgccgcc aatgattaca gttccgtcag cagcagctgg agcggttctt tcaccgcaac
     2701 ccaatatggt gcagcgtttc atcgccgcag ctccaccaat gaaccacgcc tgatggtcag
     2761 caccgatggc gtggcagata ttccggttca gggcaatctc gactacacca accattttgg
     2821 cattgcggtg gtgccgttga tttccagtta ccagccttcc accgtggcgg tgaacatgaa
     2881 tgacttaccc gacggcgtaa cagttgcaga aaacgttatc aaagaaacgt ggattgaagg
     2941 cgcgataggt tacaaatcac tggcttcccg ttccggtaaa gacggtaacg tcatcattcg
     3001 caacgccagc ggtcagtttc ctcccctcgg agcggatatc cgccaggatg acagcggcat
     3061 tagcgtgggg atggttggcg aggaaggaca tgcctggtta agcggagtcg ctgaaaatca
     3121 aaagtttacc gtggtctggg gtgatagcca gcattgctcg ctccatcttc ctgaacatat
     3181 ggaagacacc gcaaatcgcc tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca
     3241 tttatgaaag gactacccct tttgttgttg gttgccagtc tgtgcagcca cgctgcacta
     3301 caacccgatc gcactcgtat tgtgtttaac gctaatgata aagctaccag tctgcgggtg
     3361 gataatcgta gcgataaact cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg
     3421 gagaaaagcg atgatcttct ggtcgccctg ccgccgatac aacgtctgga accgaaagca
     3481 acaacacagg tacggatagt aaagcaagcc tcgaccacaa aattaccggg cgatcgcgaa
     3541 acgctatttt tctacaatat gcgtgaaatt cctcccgccc cggaaaaaaa cagcgaccat
     3601 gccgttctgc aagtcgccat tcagagtcgt atcaaggtgt tctggcgacc ggctgcatta
     3661 cgcaagaaag caggagaaaa ggtcgaactg cagttacagg tcagccagca gggcaaccaa
     3721 ctgacactga aaaatcctac cgcgtattat ctgaccattg cctacctggg acgaaatgaa
     3781 aaaggcgttc tccccggctt caaaaccgtg atggttgcgc cttttagtac ggtcaacaca
     3841 aacaccggaa attatagcgg cagccagttt tatctcggtt atatggatga ttacggcgcg
     3901 ttacgcatga cgacgctcaa ctgcagcgga caatgccatt tacaagccgt ggaggcgaag
     3961 aaatgagtgc tggcaaggga gtgttgctcg tcatctgtct gttatttctg tcgttgaagt
     4021 ctgcgcttgc cctgaactgc tactttggtt catccggtgg ctcggtagaa aaatcagaag
     4081 cgattcaacc ttttgctgta ccaggcaatg ccaaactcgg cgataagatc tgggaatctg
     4141 acgatattaa aataagatct gggaatctga cgatattaaa attcccgtct attgtgacaa
     4201 caataccaac ggcaactttg aaagcgaaca tgtctacgta tgggtaaacc cctacccggg
     4261 ggtgcaggat cgttattatc aactcggagt aacttacaat ggcgttgatt atgacgccaa
     4321 ccagggaaaa agccgcatcg acactaacca gtgtatcgac agtaagaaca ttgacattta
     4381 tacccctgat gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt
     4441 cgcgaacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg
     4501 attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta
     4561 acgaagaccc tactgcccaa aacctgaaat atcatattac tggtctggaa aacattcgcg
     4621 tgctggattg cagcgtcaat ttttccattt caccggaaac acagatgatt gattttggta
     4681 aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga
     4741 caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg
     4801 aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt
     4861 tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca
     4921 ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta
     4981 cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat
     5041 ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc
     5101 ccttttcctt gattgctgtt attttttcct agcccggctt tctttatgct gtatcgatac
     5161 accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt
     5221 gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta
     5281 cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc
     5341 cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact
     5401 ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg
     5461 acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
     5521 aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa
     5581 gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc
     5641 gcgggggccg ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac
     5701 cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca
     5761 gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg
     5821 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg
     5881 ctgctggcga tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa
     5941 atcttactgc gggcactacg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg
     6001 gctatttgtg caggtatggc gtggggactg acccggttta tgcatattga ttttatgact
     6061 gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
     6121 aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg
     6181 acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc
     6241 tggcagcctg gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg
     6301 ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
     6361 tgatgcttat tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt
     6421 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
     6481 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
     6541 gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggcga caataaccgt
     6601 tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa
     6661 aacggatgcg tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc
     6721 agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct
     6781 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaaa
     6841 ccgttgggaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
     6901 ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt
     6961 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
     7021 ggcatggtga tatccttttt tcattcttta ttaagcgggg ttatgctgcg acaacaatcc
     7081 cctttttggc aaatgtagct cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc
     7141 catgcaggca gaccgtttgc gccgcgaccg ttgcccattc gccggtgata cttttgactc
     7201 tgccgtgttg caccatctcc agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg
     7261 cgcctggctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca
     7321 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
     7381 gcccaacgag aaccaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
     7441 gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta
     7501 cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
     7561 aaacggtttc cggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac
     7621 tcgggtgagc gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatgg
     7681 tttgcgcatc gcctgcatga aatccacaag caatattggc agaggaaacc agcgttaata
     7741 gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc
     7801 agccgccacg ctaattgttc aaaataacgt tgctgatctt gccgcgcttt cagtgcctct
     7861 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
     7921 atatcagcct caatgataca ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc
     7981 atcaacacaa tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccgtga
     8041 gataacagtt cgcgatcggt gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt
     8101 cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
     8161 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
     8221 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcagtcacca
     8281 tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac
     8341 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
     8401 tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag
     8461 acggcgttat catccaaccg cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca
     8521 gtttcgaact caactgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
     8581 ttacccacca gcaggttagc aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg
     8641 atacccgact ggcaaaaacc gtgacgaccg ccatcctgca cagtggtgta catgcccgca
     8701 cgaataatct tcagcatatt ccctccttct gcggtacaaa tcgcacgctg tctcccggac
     8761 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
     8821 actgccagcc acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga
     8881 cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
     8941 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
     9001 cggaggagtg caattcaaca acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg
     9061 ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag
     9121 gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
     9181 gcgactcagg attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca
     9241 cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg
     9301 tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
     9361 gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc
     9421 acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca
     9481 gtggcatggt gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc
     9541 tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
     9601 aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg
     9661 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccattccc
     9721 ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg
     9781 atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc
     9841 aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
     9901 attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca
     9961 tccgcgccca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca
    10021 attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc
    10081 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    10141 ccttccgtat tctgatagct acaccgttaa catagcgtta catcgcctgt tcgtcgatag
    10201 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    10261 atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata aataatcaga
    10321 tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg
    10381 ttgccgtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact
    10441 atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga
    10501 ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg
    10561 cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc
    10621 tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc
    10681 tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg
    10741 gctatggcct gtttaaatct aacgtaagct gtctgctcgg cgagctgtat gagccaaccg
    10801 atccgcgtcg tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta
    10861 tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc
    10921 tggcggcggt tggaatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca
    10981 ctcatacccg cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact
    11041 ggggatggct gctggttctg ctggtggcaa cgcctgcact gattaccgtg ctgttctgga
    11101 aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa
    11161 aaatttatcg caaagcagaa aaccagaaac agcggaagga gctggggctg attgtgacgc
    11221 tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc
    11281 tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg accgcgatgt
    11341 tccagtcgat taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta
    11401 aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggccttg
    11461 gcctgatgag cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg
    11521 gtcactcttc tctgccactg atggtgttag gcctcgcggt gatgggcttt gcggaactgt
    11581 ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aattccgggc gtgaccggcg
    11641 tattaaccgg catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga
    11701 ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca
    11761 atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg
    11821 tactgatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt
    11881 cataataggc cagcaccgat gcctgatgcg acgcttgacg cgtcttatca ggcctacgta
    11941 tttcctgcaa tttattgaat ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca
    12001 tccggcatct gggctcgatt gcctgacgcg tctgttattt ccccttccgc gccgcctcat
    12061 acgctgccaa cgtttgtact ctcgcctctt tgtgctcgac tatcggttgc ggataatcca
    12121 gcttcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat
    12181 cgcgcagctc cggtaaccat cgacgaataa actcgccctc acggtcaaat ttctcgccct
    12241 gagttatcgg attgaaaata cgaaaatacg gcgcagcatc agtacccgta gaagccgccc
    12301 actgccagcc accgttatta gctgccaaat caccatcaat cagctgcgac atgaaatatc
    12361 gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct gtaatcatcc
    12421 gtagccggtt atgcatccag ccagtgctgt taagctgacg catagcggca tcaataatcg
    12481 ggtatcccgt tttgcctttc tgccaggcct gtaaatgtgc gggattgctt tgccactgca
    12541 cacgatccgt ccaggcaata aatggacgat gtttacacaa cgaggggtaa tacgtcatca
    12601 gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt
    12661 ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc
    12721 ccgttgccaa gctggcggac aaacggctgg tgccttccac tgccggaaaa tctcgttgtt
    12781 gctcatactc tccagcaccg ttctcgcaaa actggcgtaa ttgcgcaatc gccgcttttt
    12841 cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg
    12901 gcgcgggctt tatcgatccg ctactacgaa cttttggtgc agcgacgcac tccggcatcc
    12961 cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt
    13021 gattgccggt catcaccgcc cccggcggca ggatcacgct gtcatcaaat ccttcacaca
    13081 ccacgttacg cagcgctctt tcaacgtcca catcccgcgc tcgctcattc acctcatact
    13141 gatagttata aaaaagatga gtaacgctgt tttccgcgca cacctgttta actatttcga
    13201 cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt tccgccagcg
    13261 cgatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat
    13321 gcgtctccca cttgcgtggt gtagcgatat acagcgccag cacgcgtgca gacgaattgc
    13381 ggcaggcggc agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccaggcca
    13441 gatgggtagt cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa
    13501 ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat
    13561 cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg cagccagctg
    13621 ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt
    13681 ggtgtgatcg cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggcggtactg
    13741 gttgtaaaaa tcatccagat tttgccactg atgaatatca gccacaaacg ggccaagttt
    13801 gcggtactcg ggctgagaat gggccagaaa gacgagttta tagcggctgt gataatcgag
    13861 aagcgccccg cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat
    13921 acggattagg gtgctgtcat caaatcgttg tagattcatt actgctcctg aacggtatac
    13981 aggagtaagt gtaggcgttg gtaaatgatg tttgtgaagt taatccgcta gcagggcaag
    14041 attacgaaag cgcgctcccc gcaagcaact aatcaaggta gttaatcatg gctgaacacg
    14101 atattacgcc agaagtcact atttccaaaa cacagcgccg ctataaagtc ggctatgtca
    14161 gagcccgcca ccaggaccgc aaaaccggta tagcccggta ttattcacaa catcccagtc
    14221 tgcaccttaa gggcgactgg ctgaaagagg ccggatttga taccggacgg ggtgtcacgg
    14281 tgaagatttc ggaggggggc ctgaccatca tcgctgacag tgacgaggtg caggaactgc
    14341 gggatgggat gtttagtgtg ctgaacgaaa gttaaatatt aaaaaagctg g
//
LOCUS       DAZPDE010000098        14191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPDE010000098 DAZPDE010000000
VERSION     DAZPDE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.098"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHU0J_002645"
     CDS             <1..79
                     /locus_tag="ACHU0J_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217279.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHU0J_002646"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHU0J_002646"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1217280.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHU0J_002647"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHU0J_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1217281.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHU0J_002648"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHU0J_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1217282.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHU0J_002649"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHU0J_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1217283.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHU0J_002650"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHU0J_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1217284.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHU0J_002651"
     CDS             5377..5835
                     /locus_tag="ACHU0J_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1217285.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHU0J_002652"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHU0J_002652"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1217286.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHU0J_002653"
     tRNA            6887..6971
                     /locus_tag="ACHU0J_002653"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHU0J_002654"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHU0J_002654"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHU0J_002655"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHU0J_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1217287.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHU0J_002656"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHU0J_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1217288.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHU0J_002657"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHU0J_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1217289.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHU0J_002658"
     CDS             complement(10252..13995)
                     /locus_tag="ACHU0J_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1217290.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3508 a         3750 c         3405 g         3528 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg t
//
LOCUS       DAZPDE010000099        14110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPDE010000099 DAZPDE010000000
VERSION     DAZPDE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.099"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..415
                     /locus_tag="ACHU0J_002659"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHU0J_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHU0J_002660"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHU0J_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR1217291.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHU0J_002661"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHU0J_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHU0J_002662"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHU0J_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHU0J_002663"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHU0J_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHU0J_002664"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHU0J_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHU0J_002665"
     CDS             complement(7513..8094)
                     /locus_tag="ACHU0J_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217292.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHU0J_002666"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHU0J_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1217293.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHU0J_002667"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHU0J_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHU0J_002668"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHU0J_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHU0J_002669"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHU0J_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1217294.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3459 g         3793 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZPDE010000100        13981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPDE010000100 DAZPDE010000000
VERSION     DAZPDE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.100"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHU0J_002670"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHU0J_002670"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1217295.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHU0J_002671"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHU0J_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1217296.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHU0J_002672"
     CDS             2630..3391
                     /locus_tag="ACHU0J_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1217297.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHU0J_002673"
     CDS             complement(3442..4326)
                     /locus_tag="ACHU0J_002673"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1217298.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHU0J_002674"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHU0J_002674"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1217299.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHU0J_002675"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHU0J_002675"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1217300.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHU0J_002676"
     CDS             6202..6474
                     /locus_tag="ACHU0J_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1217301.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHU0J_002677"
     CDS             6844..7920
                     /locus_tag="ACHU0J_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1217302.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHU0J_002678"
     CDS             7947..9326
                     /locus_tag="ACHU0J_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217303.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHU0J_002679"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHU0J_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHU0J_002680"
     CDS             10409..11245
                     /locus_tag="ACHU0J_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR1217304.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHU0J_002681"
     CDS             11250..12197
                     /locus_tag="ACHU0J_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1217305.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHU0J_002682"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHU0J_002682"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR1217306.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..>13981
                     /locus_tag="ACHU0J_002683"
     CDS             13324..>13981
                     /locus_tag="ACHU0J_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1217307.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     Q"
BASE COUNT         3665 a         3103 c         3569 g         3644 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 t
//
LOCUS       DAZPDE010000101        13178 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPDE010000101 DAZPDE010000000
VERSION     DAZPDE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.101"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(160..480)
                     /locus_tag="ACHU0J_002684"
                     /pseudo
     CDS             complement(160..480)
                     /locus_tag="ACHU0J_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(686..1405)
                     /locus_tag="ACHU0J_002685"
                     /pseudo
     CDS             complement(686..1405)
                     /locus_tag="ACHU0J_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1421..1753)
                     /gene="cusF"
                     /locus_tag="ACHU0J_002686"
     CDS             complement(1421..1753)
                     /gene="cusF"
                     /locus_tag="ACHU0J_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1217308.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1777..3159)
                     /gene="cusC"
                     /locus_tag="ACHU0J_002687"
     CDS             complement(1777..3159)
                     /gene="cusC"
                     /locus_tag="ACHU0J_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1217309.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3316..3999
                     /gene="cusR"
                     /locus_tag="ACHU0J_002688"
     CDS             3316..3999
                     /gene="cusR"
                     /locus_tag="ACHU0J_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1217310.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3989..5431
                     /gene="cusS"
                     /locus_tag="ACHU0J_002689"
     CDS             3989..5431
                     /gene="cusS"
                     /locus_tag="ACHU0J_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1217311.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5581..7818
                     /gene="nrfB"
                     /locus_tag="ACHU0J_002690"
     CDS             5581..7818
                     /gene="nrfB"
                     /locus_tag="ACHU0J_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR1217312.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7805..10773
                     /gene="nfrA"
                     /locus_tag="ACHU0J_002691"
                     /pseudo
     CDS             7805..10773
                     /gene="nfrA"
                     /locus_tag="ACHU0J_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10774..11664
                     /locus_tag="ACHU0J_002692"
     CDS             10774..11664
                     /locus_tag="ACHU0J_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1217313.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11847..12608
                     /gene="envY"
                     /locus_tag="ACHU0J_002693"
     CDS             11847..12608
                     /gene="envY"
                     /locus_tag="ACHU0J_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1217314.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12654..12730)
                     /locus_tag="ACHU0J_002694"
     tRNA            complement(12654..12730)
                     /locus_tag="ACHU0J_002694"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12694..12696),aa:Arg,seq:tct)
     gene            12973..>13178
                     /locus_tag="ACHU0J_002695"
     CDS             12973..>13178
                     /locus_tag="ACHU0J_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR1217315.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3234 a         3415 c         3348 g         3181 t
ORIGIN      
        1 acgtgggtgg actggtataa caatcgacga ttgctggaaa ggctgggcca catccctccg
       61 gcagaagcag aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acactctcca ggaaacccgg ggcggttcag ttgtcgaacc agtagtcgag
      181 acactgggcg accgtcatgg agttctccac cttctcttca aaataagtgc gtggatccgt
      241 tcctgagaaa tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag
      301 ggatgggtat cgaccaagcc caagtcgatt aggcttgcca tgccagcgat agcggtactg
      361 gaactggatg acccccttcg gtgaaattcg tacgctgagg ccatcagcat cagccaattc
      421 ttgtgggcca gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat
      481 aaataatatt tggtacgctc agaaataatg gtttggtaca catctggtac acaatatcac
      541 atggacaaca gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa
      601 aaaaggttgt ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac
      661 aatgacagac acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga
      721 tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg
      781 taaagcgagt ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc
      841 ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc
      901 gcaacagtaa atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg
      961 gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg
     1021 cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc gtcgggaaac
     1081 tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg
     1141 tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca
     1201 ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca
     1261 tcgggtcgta ccagaataag attttacgtt ctgcggtcgg cattttttct gcgtgcggtt
     1321 ccgcctggtc aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc
     1381 cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat
     1441 cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt
     1501 taatttcact cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt
     1561 tgacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt
     1621 ttaccacacc ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat
     1681 gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca
     1741 gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa
     1801 ggcggtatac aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg
     1861 tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg
     1921 ctgatataat gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg
     1981 ggcgctgatt tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa
     2041 ggcgttctgg attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat
     2101 atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa
     2161 attccacatc ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat
     2221 tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt
     2281 agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga
     2341 cgacaagccc gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg
     2401 cggtagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc
     2461 cccctgacgt ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag
     2521 cgccagaaca ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg
     2581 ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa
     2641 atagctttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt
     2701 ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag
     2761 atcaaagctg gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag
     2821 attgccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt cggcatcggt
     2881 cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt
     2941 attcaccagc gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca
     3001 gcccacgttc tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg
     3061 ctgcggtacg ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc
     3121 aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat
     3181 tttatgtcgc caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa
     3241 ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca
     3301 acatgcggag gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac
     3361 ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat
     3421 ggctaccatc tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca
     3481 gacgtgaacg gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt
     3541 ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca
     3601 gatgactacc tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc
     3661 ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc
     3721 gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagaa
     3781 tttactctgc tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc
     3841 gcctcgcagg tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg
     3901 aagctgctac gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc
     3961 ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc
     4021 gctggcaacc cgcctgacct tttttatcag ccaggccacc atcgcggcgt ttttcgcctt
     4081 tgcatggatc ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt
     4141 aaaagagatt agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg
     4201 acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc
     4261 agatagccac ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc
     4321 acgtgatgcc atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc
     4381 gacgattatg atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat
     4441 taacttgccg gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct
     4501 gtcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc
     4561 atcggtaatc agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc
     4621 gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg
     4681 ccttgacccg cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat
     4741 gatcgagcgt attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca
     4801 cgaaattcgc acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc
     4861 gcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat
     4921 ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc
     4981 cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc
     5041 gctagcggaa gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg
     5101 cgatccgttg atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac
     5161 gccaaccaga gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc
     5221 cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt
     5281 ctatcgcgtt gacccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat
     5341 agtgaaatcg attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg
     5401 gacaaggttt gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc
     5461 agtgtaaaaa taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt
     5521 cataaaaaac cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt
     5581 gtggactggc ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata
     5641 acgttagcgg tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac
     5701 tgggtacgcc gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac
     5761 cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa
     5821 acgggcgtca tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat
     5881 catatctttg ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg
     5941 tgcgctcgct ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa
     6001 gccgactgcc tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc
     6061 gcttttgctg gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt
     6121 ctgttcaact atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa
     6181 cgcgaatgga cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc
     6241 aaagatgttc cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc
     6301 tgtttcagcc gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac
     6361 gtgcagagtc ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg
     6421 gaaatttttg tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt
     6481 ttacagcacg cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt
     6541 tcgactgcgg ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa
     6601 acccataaat ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg
     6661 gcaatcagta actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg
     6721 ctggcgtatg aaagtttgtg gcccgatgcc tggcatttcc tttctatttt tagcggcagc
     6781 gcatggttaa tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag
     6841 cgagtgattt tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt
     6901 cttttctggg gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt
     6961 cagcacggcg atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc
     7021 actggcgata cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc
     7081 actgaagaac aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt
     7141 tcaatgctga tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa
     7201 aacggcgtgg cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa
     7261 atgccagcct ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag
     7321 ttaattgtcg gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa
     7381 gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc
     7441 cactggtatg cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag
     7501 catcagtggc tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag
     7561 ttcctgttcg ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg
     7621 ttgttattgc gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc
     7681 gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg
     7741 atgcaatcac tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc
     7801 gaaaatgaag gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc
     7861 tttattgagt accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct
     7921 gagcgattat cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa
     7981 aaataacgac gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga
     8041 taatattccg ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg
     8101 ggcgcggctg ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg
     8161 cagtctggcg gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc
     8221 ccagcaaaaa gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa
     8281 tgccctgcgg ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc
     8341 tgcatcgccg gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa
     8401 acaatgatcc caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc
     8461 ggcagaacac cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct
     8521 ggcactgcaa tcacagggga tcttcaccga tcctcagtca tatattactt acgcgaccgc
     8581 gctggcttat cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct
     8641 gtttaccacg ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa
     8701 ccccgttcag gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt
     8761 tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct
     8821 cgccaccctc cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg
     8881 taacaaggct gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct
     8941 tccccgcctg gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc
     9001 cgatttattg ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat
     9061 ggcgcgactg gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc
     9121 gattttatcg aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg
     9181 tattgcagat aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga
     9241 tgctgccgcc tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt
     9301 gtatgcatgg cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc
     9361 ctatcaggcg tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag
     9421 tcttcacgac atgagcaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg
     9481 aaatggcgca gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa
     9541 tgctcaatac tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact
     9601 gaacgatctc acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc
     9661 gacaatttat cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct
     9721 ggaactggaa ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag
     9781 cggtgatatc gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga
     9841 tccggcactg atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac
     9901 gcagcactac gcccggctcg tgattgatga cattgataat caggcataac cccactgacc
     9961 ccagagcaaa atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc
    10021 tggacgttca gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac
    10081 aataatgtcg gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc
    10141 gagtaccgca tcggacgcaa tatgctgctg gaaggcgacc tgctctcagt ttatagccgc
    10201 gtctttgccg ataccggaga aaacggggtg atgatgccgg tgaaaaatcc gatgtccggc
    10261 accggtctgc gctggaagcc gctgcgcgat cagatctttt tcctcgccgt cgaacagcag
    10321 ttgccgctga acgggcaaaa tggcgcatcc gataccatgc tgcgcgccag cgcctcattc
    10381 tttaatggcg gcaaatacag cgacgaatgg cacccgaacg gttcaggctg gtttgcccaa
    10441 aacctgtacc tcgatgcggc gcaatatatc cgccaggata ttcaggcgtg gacggcagat
    10501 tatcgcgtca gctggcatca gaaggtagct aacggacaga ctattgagcc ttacgctcac
    10561 gttcaggaca acggctatcg tgataaaggc actcagggcg cgcagcttgg cggtgtcggg
    10621 gtccgctgga atatttggac cggcgagacg cactacgacg cctggccgca caaagtcagt
    10681 ctcggcgtcg agtatcaaca tacctttaag gcgattaatc aacgtaacgg agagcgcaac
    10741 aacgcgtttc tcaccattgg agtgcactgg taaatgcgta agttcatttt cgtattgctg
    10801 acactgcttt tggtcagccc tttttccttt gcgatgaaag gtattatctg gcaaccacaa
    10861 aaccgagata gtcaggttac cgatacccag tggcaggggc tgatgagtca gttacgtttt
    10921 caaggcttcg ataccctggt tttgcaatgg acccgttacg gcgatgcatt tacccagcca
    10981 gaacagcgca cgttattgtt taagcgtgcc gcagctgcgc aacagactgg cctgaagctg
    11041 attgtcgggc tgaacgccga tccggaattt tttatgcacc agaaacagtc gtctgcagcg
    11101 ctggaaagct atcttaatcg cctgctggct gccgatctcc agcaagccag attatggagc
    11161 gccgcgcctg gcataacgcc ggatggctgg tacatcagcg cggaaattga cgacctgaac
    11221 tggcgcagcg aagccgcccg tcaacctttg ctaacatggt taaacaacgc gcagcggctg
    11281 attagcgatg tttcagcaaa accggtttat atcagtagtt ttttcgccgg aaacatgtcg
    11341 cccgatggct atcgccaact gctggaacaa gttaaagcaa ccggagttaa tgtctgggta
    11401 caggatggca gcggcgtgga taaacttacc gctgaacagc gtgaacgtta tctacaggcc
    11461 agcgccgatt gccaaagctc cgccccagca agcggcattg tttatgaact gtttgtcgcc
    11521 ggcaaaggca aaacctttac agcgaaaccg aaaccggacg cagaaattgc ctcgctgtta
    11581 gcgaaacgtt cctcttgcgg taaagacatg ctctatttct ctctgcgcta tttgcccgtc
    11641 gcgcacggca ttctcgagta ttaaatcccc tccaggtaag tcgggtacga cctggcttac
    11701 ctctttcgct ctcgtcagaa ttccatcaag attaattgct aaaaagcatg ctaaagaaag
    11761 aagatcgtta tcggcgaacg ggccgatcac tcatgcagga agcatagatc ctttgagcgg
    11821 agtttctgat atctgaggag tgcgaaatgc aattgagcag cagtgaacct tgcgtggtga
    11881 tcctgaccga aaaagaggta gaggtaagcg tcaataacca tgctacgttt acccttccga
    11941 aaaactacct ggccgccttc gcgtgcaaca ataacgtcat tgaactctca acgttaaatc
    12001 acgtattaat cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata
    12061 agaaattaac ctgtgtaaag ccctggtcgc ggctagcaac cccggttatc gcttgtcata
    12121 gccgtacacc ggaagtgttc cggctagcca ccaaccacag caagcagcaa tccagcaaac
    12181 cctgcgaagc ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc
    12241 aatcgcggtt tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct
    12301 gccgcattat tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc
    12361 tatgtttaag ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc
    12421 agattgtcac agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa
    12481 atatcactca ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt
    12541 ttaaggcgtt ttacagcctg acaccgttga attatctcgc caaacagcga caaaaagtga
    12601 tgtggtgaag ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg
    12661 ccctgcagga ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag
    12721 ctaagggcgc gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg
    12781 ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg
    12841 atttatttcg ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc
    12901 cgtattttca tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg
    12961 agactggtaa ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga
    13021 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
    13081 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
    13141 atagacttgc ccggaacaga cggttttacc ttcctgaa
//
LOCUS       DAZPDE010000102        13177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPDE010000102 DAZPDE010000000
VERSION     DAZPDE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.102"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHU0J_002696"
     CDS             complement(<1..1177)
                     /locus_tag="ACHU0J_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1217316.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHU0J_002697"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHU0J_002697"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHU0J_002698"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHU0J_002698"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1217317.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHU0J_002699"
     CDS             2804..4306
                     /locus_tag="ACHU0J_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1217318.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHU0J_002700"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHU0J_002700"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1217319.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHU0J_002701"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHU0J_002701"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1217320.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHU0J_002702"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHU0J_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1217321.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHU0J_002703"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHU0J_002703"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1217322.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHU0J_002704"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHU0J_002704"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1217323.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHU0J_002705"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHU0J_002705"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1217324.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHU0J_002706"
     CDS             complement(11871..13067)
                     /locus_tag="ACHU0J_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1217325.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
BASE COUNT         3399 a         3402 c         3415 g         2961 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttat
//
LOCUS       DAZPDE010000103        13098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPDE010000103 DAZPDE010000000
VERSION     DAZPDE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.103"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHU0J_002707"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHU0J_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1217326.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHU0J_002708"
     CDS             1131..1931
                     /locus_tag="ACHU0J_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1217327.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHU0J_002709"
     CDS             2009..2779
                     /locus_tag="ACHU0J_002709"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1217328.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHU0J_002710"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHU0J_002710"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1217329.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHU0J_002711"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACHU0J_002711"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1217330.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACHU0J_002712"
     CDS             5034..5756
                     /locus_tag="ACHU0J_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1217331.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHU0J_002713"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACHU0J_002713"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1217332.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHU0J_002714"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACHU0J_002714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1217333.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACHU0J_002715"
     tRNA            7149..7225
                     /locus_tag="ACHU0J_002715"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACHU0J_002716"
     CDS             complement(7356..8402)
                     /locus_tag="ACHU0J_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR1217334.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHU0J_002717"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACHU0J_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHU0J_002718"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACHU0J_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1217335.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHU0J_002719"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACHU0J_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1217336.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACHU0J_002720"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACHU0J_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHU0J_002721"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACHU0J_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZPDE010000104        13001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPDE010000104 DAZPDE010000000
VERSION     DAZPDE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.104"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            335..916
                     /gene="mdaB"
                     /locus_tag="ACHU0J_002722"
     CDS             335..916
                     /gene="mdaB"
                     /locus_tag="ACHU0J_002722"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1217337.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            947..1261
                     /locus_tag="ACHU0J_002723"
     CDS             947..1261
                     /locus_tag="ACHU0J_002723"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1217338.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1309..3201)
                     /gene="parE"
                     /locus_tag="ACHU0J_002724"
     CDS             complement(1309..3201)
                     /gene="parE"
                     /locus_tag="ACHU0J_002724"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1217339.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3230..3811)
                     /gene="yqiA"
                     /locus_tag="ACHU0J_002725"
     CDS             complement(3230..3811)
                     /gene="yqiA"
                     /locus_tag="ACHU0J_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1217340.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3811..4638)
                     /gene="cpdA"
                     /locus_tag="ACHU0J_002726"
     CDS             complement(3811..4638)
                     /gene="cpdA"
                     /locus_tag="ACHU0J_002726"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1217341.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4663..5085)
                     /locus_tag="ACHU0J_002727"
     CDS             complement(4663..5085)
                     /locus_tag="ACHU0J_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1217342.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5086..5715)
                     /gene="nudF"
                     /locus_tag="ACHU0J_002728"
     CDS             complement(5086..5715)
                     /gene="nudF"
                     /locus_tag="ACHU0J_002728"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1217343.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5920..7401
                     /gene="tolC"
                     /locus_tag="ACHU0J_002729"
     CDS             5920..7401
                     /gene="tolC"
                     /locus_tag="ACHU0J_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1217344.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7549..8220
                     /locus_tag="ACHU0J_002730"
     CDS             7549..8220
                     /locus_tag="ACHU0J_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1217345.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8226..9386
                     /locus_tag="ACHU0J_002731"
     CDS             8226..9386
                     /locus_tag="ACHU0J_002731"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1217346.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9424..10212)
                     /gene="ygiD"
                     /locus_tag="ACHU0J_002732"
     CDS             complement(9424..10212)
                     /gene="ygiD"
                     /locus_tag="ACHU0J_002732"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1217347.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10355..11128
                     /gene="zupT"
                     /locus_tag="ACHU0J_002733"
     CDS             10355..11128
                     /gene="zupT"
                     /locus_tag="ACHU0J_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1217348.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11186..11356)
                     /gene="yqiD"
                     /locus_tag="ACHU0J_002734"
     CDS             complement(11186..11356)
                     /gene="yqiD"
                     /locus_tag="ACHU0J_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1217349.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11617..12270)
                     /gene="ribB"
                     /locus_tag="ACHU0J_002735"
     CDS             complement(11617..12270)
                     /gene="ribB"
                     /locus_tag="ACHU0J_002735"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1217350.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12374..12522)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12644..12934
                     /gene="ubiK"
                     /locus_tag="ACHU0J_002736"
     CDS             12644..12934
                     /gene="ubiK"
                     /locus_tag="ACHU0J_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1217351.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3294 a         3479 c         3159 g         3069 t
ORIGIN      
        1 ttttctgctt taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga
       61 cacgttgata ttttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc
      121 gaaccaaacg tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat
      181 tgtcattttc agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc
      241 taaattcgca aaagactttg cacattttgc taatttcaca gtaccgctct gtgacgtact
      301 atagtcgaca aacgtctcac cttgagatta aaaaatgagc aacatcctga ttatcaacgg
      361 cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga
      421 tggcacactg cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacaa
      481 tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg
      541 ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg
      601 tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg
      661 ttccggcagc ctggtacagg acaaaaaata tatgctttct ctaacctgga acgcaccaat
      721 ggaagccttc accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct
      781 gccgttccat aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa
      841 tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt
      901 ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat
      961 cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc
     1021 taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga
     1081 ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca
     1141 gtgggaaagc atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag
     1201 cgaagccgta aaaggcgacg tgctggagat gaatatccgt attctgcagc cagggattta
     1261 atcctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat
     1321 ctccgccatg tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc
     1381 cagcagcata tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg
     1441 caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc
     1501 ccccagacct ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag
     1561 tacgccctct ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg
     1621 gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt
     1681 tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag
     1741 gatacagatt ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat
     1801 cgctaccgaa atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca
     1861 ggtgttaagg atcttacctt tcagcggcat gatcgcctga tattcacgat cgcgcgcctg
     1921 cttggcagat ccgcctgcgg agtcaccttc cacgaggaac agttcggtac ggttaaggcc
     1981 ctgcgcggta caatcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg
     2041 caccactttt ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag
     2101 caactccgcc gcctgaacgt tctggttcag ccacaggatg aaggcatctt tcaccacgcc
     2161 cgaaacgaat gccgcacact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg
     2221 cggatcctgc attttaaccg acagaacata ggcgcagcga tcccagatat cttccgccga
     2281 cagcttcacg ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa
     2341 caggccctga cgcagaccat taacatgggt accgccctgc atcgttggga taaggttgac
     2401 gtagctttcg gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc
     2461 ttcagtatcg ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt
     2521 taccgcttcc gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt
     2581 gttgatctca tctttaaagg taatctcaac gccggggcac agtactgctt tggccttcag
     2641 cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca
     2701 gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg
     2761 caccttttcg ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac
     2821 ttctacgcgc ttcgacaagg cgttaactac cgaaatcccc acgccatgca ggccgccaga
     2881 gaactggtaa tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc
     2941 aaccgccggt actccttctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat
     3001 cacttctaac gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag
     3061 tgcttcatcg acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc
     3121 ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc
     3181 agcgttataa gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt
     3241 gcagaccaag aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt
     3301 tgccgccttc tatgacagtc tggcggcagg aagcatagta cgccaccgcc tggcggtaat
     3361 ccagcacttc atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt
     3421 caatctgcat gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg
     3481 tgtaggggtt ctcgttctga ccgagatagt ccgtcagcaa ttcaaacggg cgtaccgccg
     3541 ggtttaccac cactgcgggc agcataaaac attgcgacaa ccaggtggcg taatatcccc
     3601 ccagtgacga accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca
     3661 gcagttctgc cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag
     3721 ggtgatgttc cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt
     3781 tgaaaccgtg taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg
     3841 gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc
     3901 gagagtacgc cagccgggcg cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa
     3961 ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc
     4021 ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc
     4081 cagttcgccc gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg
     4141 atgatgatgc agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag
     4201 ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag
     4261 caacaggatt tgccattgct caccaataaa cacccgcttc gccggggaga tacccgcatc
     4321 ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg
     4381 cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg
     4441 cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc
     4501 cagcaccgcc tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc
     4561 aaacaggtga gtatcggtaa tttgtaaaat ccttcctctg gcctcaccag ccagaggaag
     4621 ggttaacagg ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc
     4681 catcgctcca tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg
     4741 cttttcgtcg cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa
     4801 aatctgctgg cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat
     4861 tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt
     4921 gtatcgggtc gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac
     4981 agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc
     5041 gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt
     5101 ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc
     5161 gattttcccc tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat
     5221 atcttcgttt tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc
     5281 caccataatt gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg
     5341 tttggtccgt ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc
     5401 cacactttca ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcgct
     5461 ggtgtcgtac gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc
     5521 aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact
     5581 catttgcccg ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg
     5641 ataaagtgtt tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt
     5701 gtctggctta agcattgtta atgtcctagc actaatagtg aattaaatgt gaatttcagc
     5761 gacgtttgac tgccgtttga gcagtcatat gttaaattga ggcacattaa cgccctatgg
     5821 cacgtaacgc caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt
     5881 ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt
     5941 cttatcggcc tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa
     6001 gtttatcagc aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat
     6061 gctgcctttg aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt
     6121 gcagattaca cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc
     6181 agtgcgtccc tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg
     6241 ctgcaggaaa aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg
     6301 atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat
     6361 acacaggcac aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac
     6421 gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg
     6481 gcgaacgaag tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc
     6541 accggtaact actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa
     6601 ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag
     6661 gcacgcttga gccaggacct ggagcgcgag caaattcgcc aggcgcagga tggtcactta
     6721 ccgactctgg atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa
     6781 acccgtggtg ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc
     6841 ctgagcttct cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca
     6901 cagtacaact ttgttggtgc cagtgagcaa ctggaaagcg cacatcgtag cgtcgtgcaa
     6961 accgtacgtt cctccttcaa caacattaat gcttctatca gtagtattaa cgcctacaaa
     7021 caagccgtag tttccgcgca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt
     7081 acgcgtacca ttgttgatgt gttggatgcg accaccacgc tgtacaacgc caagcaagag
     7141 ctggcgaatg cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt
     7201 acgttgaacg agcaggattt gctggcactg aacaatgcgc tgagcaaacc ggtttccact
     7261 aatccggaaa acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg
     7321 cctgatagcc cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt
     7381 cataaccctt tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc
     7441 aacgtaaaga tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag
     7501 ccatcctcta ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca
     7561 aaatccatac gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc
     7621 gctctcgcgg ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg
     7681 tctctctatc aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt
     7741 accaccgcgt acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc
     7801 cgtgaagact gtgttgctga atttggtgaa ggtcagtgtc agcaagcacc agcccaggct
     7861 ggcatggcac cagaaaacca ggcgcaggct cagcaatcca gcgggagttt ctggatgccg
     7921 ctgatggccg gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag
     7981 ccgctgttct cctcgaaaaa cccggccagt ccggcttacg gtaaatatac cgacgcgacg
     8041 ggtaaaaact atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg
     8101 gcaccaaaac cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc
     8161 aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat gggtggctga
     8221 taccgatgga aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat
     8281 acggtttcaa ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca
     8341 agttgaccct cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt
     8401 gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac
     8461 caaaacacac ctggagtttt gtgcgtcaat catggctgac tcaccagcca tcgctttatt
     8521 cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg
     8581 atacgccaac gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc
     8641 ttaacgccgg taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga
     8701 tcgatcgctt cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct
     8761 gtcgcgacac ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaaccgaag
     8821 ctgaaatcgc caccgagttc ctctacatcg acgagatagg tttaggcgaa aaaggccagt
     8881 tcacggattt acaggatcag gtgatttcta acctgttcaa gctttatccg tgggaattta
     8941 tgctgcgcga aatgttctcc accaagctgg aagatgctgg cgtacgctgg ctggaaccgg
     9001 cgtggaagag cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga
     9061 atcacccgaa cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat
     9121 atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca
     9181 aaaccattga agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc
     9241 acccgttacc gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc
     9301 aacctgccgg aattggcatt cgtgaagacc gtgcgttgat cacccaggat atgtctcggt
     9361 tttatccaca tatttttgtt gaataagcca cgataccgga tggcactcgc catccggtaa
     9421 ttgttagcct atctgcaccg acagcatact taaactgccc atttctatac catcgaccgg
     9481 aatggtaatt ggctcctgcc catcccacac acctaacaca tacaacaacg gcagatagtg
     9541 ctctggcgtt gggttcgata acgcgccacc ttcatggtcg agataattca ccagaggatg
     9601 ctgctctacc ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc
     9661 ccacggatac ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt
     9721 accgctggcg accaacatta ttccttcatc acgcagcgcc gcgagtttgc gccccatttc
     9781 gaaatgccag gcggcaggtt tgctactgtc gatactcaac tgcaccatag ggatatcggc
     9841 gtcaggatac atcttaatca gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt
     9901 atccagcgtc accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga
     9961 acccggagca ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat
    10021 cgtgggcggc gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac
    10081 caccacaatc gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata
    10141 caaattatct tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg
    10201 tgttgaagac atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc
    10261 ttgttcggcg gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat
    10321 ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat
    10381 actggcgggg gcagccacgt ttattggcgc gtttctcggc gttctcggac aaaaaccctc
    10441 gaaccgctta ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt
    10501 aatggaaatg cttcctgccg cactagcagc tgaaggaatg tcgccggtgt tgggttatgg
    10561 aatgtttatc ttcggtctgc ttggctactt tggcctggac cgcatgttgc cgcatgcgca
    10621 tccgcaggat ttaatgcaaa aatcggtgca gctgttgcca aaatcgatca agcgcacagc
    10681 cattctgctc actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt
    10741 cacggcgagc agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa
    10801 tatccctgaa ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac
    10861 cgcgattctg tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg
    10921 gttaatcctc ggtagcatga tttccccggt ggtcatggcg gcaatcatgg cggcggttgc
    10981 aggaattatg gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc
    11041 taataataac cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt
    11101 gctgctacaa acggcgggaa ttggttaaaa acaacatcag aagttgtcca gcacatggac
    11161 aacttctgcc agcttcacga aagccttatt ctaccttttc ggctgtctct tctctcgtac
    11221 tgtttaagta tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac
    11281 aataacgttt caaatgcagg aaataaccca ccacaaccag agcaatcaat acaatccagt
    11341 accaggcgat aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata
    11401 aatttcacat atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata
    11461 tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag
    11521 aagcggaatt aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc
    11581 ggtttcttga ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc
    11641 ctgacggtat gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa
    11701 ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac
    11761 accagccggt ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc
    11821 acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag
    11881 atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc
    11941 agaaacaccg gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata
    12001 ggcgctggtg ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt
    12061 gatgcacagg caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt
    12121 catggtttct gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac
    12181 cattacaccg cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt
    12241 accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta
    12301 ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca
    12361 tgcccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca
    12421 ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga
    12481 tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta
    12541 atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg
    12601 cactacaatg gtactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa
    12661 aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg
    12721 ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct
    12781 tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact
    12841 ggcgctgctt gaacaaagaa tcagcgaact ggaaaatcgc agtactgaga tcaaaaagca
    12901 gccagatcca gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat
    12961 tatgaattaa tcctctaatt tacagtgttt tctattacat t
//
LOCUS       DAZPDE010000105        12908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPDE010000105 DAZPDE010000000
VERSION     DAZPDE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.105"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHU0J_002737"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHU0J_002737"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1217352.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHU0J_002738"
     CDS             complement(1806..3224)
                     /locus_tag="ACHU0J_002738"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1217353.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHU0J_002739"
     CDS             complement(3378..3722)
                     /locus_tag="ACHU0J_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1217354.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHU0J_002740"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHU0J_002740"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1217355.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHU0J_002741"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHU0J_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHU0J_002742"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHU0J_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1217356.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHU0J_002743"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHU0J_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1217357.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHU0J_002744"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHU0J_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1217358.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHU0J_002745"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHU0J_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1217359.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHU0J_002746"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHU0J_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1217360.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHU0J_002747"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHU0J_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1217361.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHU0J_002748"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHU0J_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR1217362.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHU0J_002749"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHU0J_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHU0J_002750"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHU0J_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR1217363.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHU0J_002751"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHU0J_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZPDE010000106        12733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPDE010000106 DAZPDE010000000
VERSION     DAZPDE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.106"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHU0J_002752"
     CDS             <1..1038
                     /locus_tag="ACHU0J_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1217364.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACHU0J_002753"
     CDS             1039..3204
                     /locus_tag="ACHU0J_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR1217365.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKERYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACHU0J_002754"
     CDS             3218..4129
                     /locus_tag="ACHU0J_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR1217366.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACHU0J_002755"
     CDS             4129..5424
                     /locus_tag="ACHU0J_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR1217367.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACHU0J_002756"
     CDS             5469..5699
                     /locus_tag="ACHU0J_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217368.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACHU0J_002757"
     CDS             5677..6177
                     /locus_tag="ACHU0J_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR1217369.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACHU0J_002758"
     CDS             6177..7595
                     /locus_tag="ACHU0J_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR1217370.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACHU0J_002759"
     CDS             7595..8443
                     /locus_tag="ACHU0J_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR1217371.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACHU0J_002760"
     CDS             8443..8898
                     /locus_tag="ACHU0J_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR1217372.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACHU0J_002761"
     CDS             8901..9593
                     /locus_tag="ACHU0J_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR1217373.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACHU0J_002762"
     CDS             9603..10934
                     /locus_tag="ACHU0J_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR1217374.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACHU0J_002763"
     CDS             10935..12665
                     /locus_tag="ACHU0J_002763"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1217375.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3435 a         3145 c         3355 g         2798 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 cgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAZPDE010000107        12700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPDE010000107 DAZPDE010000000
VERSION     DAZPDE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.107"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHU0J_002764"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHU0J_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1217376.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHU0J_002765"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHU0J_002765"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1217377.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHU0J_002766"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHU0J_002766"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1217378.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHU0J_002767"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHU0J_002767"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1217379.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHU0J_002768"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHU0J_002768"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1217380.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHU0J_002769"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHU0J_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHU0J_002770"
     CDS             7849..7902
                     /locus_tag="ACHU0J_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217381.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHU0J_002771"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHU0J_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1217382.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHU0J_002772"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHU0J_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1217383.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHU0J_002773"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHU0J_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1217384.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHU0J_002774"
     CDS             10966..12165
                     /locus_tag="ACHU0J_002774"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1217385.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3122 a         3176 c         3374 g         3028 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    12301 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    12361 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    12421 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    12481 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    12541 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    12601 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    12661 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAZPDE010000108        12435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPDE010000108 DAZPDE010000000
VERSION     DAZPDE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.108"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="ACHU0J_002775"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="ACHU0J_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1217386.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="ACHU0J_002776"
     CDS             complement(2051..2974)
                     /locus_tag="ACHU0J_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1217387.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="ACHU0J_002777"
     CDS             3191..4534
                     /locus_tag="ACHU0J_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1217388.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="ACHU0J_002778"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="ACHU0J_002778"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1217389.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="ACHU0J_002779"
     CDS             6554..6778
                     /locus_tag="ACHU0J_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1217390.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="ACHU0J_002780"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="ACHU0J_002780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1217391.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /gene="ydcK"
                     /locus_tag="ACHU0J_002781"
     CDS             complement(7372..8352)
                     /gene="ydcK"
                     /locus_tag="ACHU0J_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1217392.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="ACHU0J_002782"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="ACHU0J_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1217393.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="ACHU0J_002783"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="ACHU0J_002783"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1217394.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10361..11017
                     /locus_tag="ACHU0J_002784"
     CDS             10361..11017
                     /locus_tag="ACHU0J_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR1217395.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="ACHU0J_002785"
                     /pseudo
     CDS             complement(11035..11541)
                     /gene="tnpA"
                     /locus_tag="ACHU0J_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11549..12435
                     /locus_tag="ACHU0J_002786"
                     /pseudo
     CDS             11549..12435
                     /locus_tag="ACHU0J_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3244 a         2913 c         3120 g         3158 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctgacacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta
    11401 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
    11461 gactaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
    11521 tttcacagac caaagtatca tttgacctgt gaaacgatta caggcattta aattccagtt
    11581 aagaccaggt ggtcaacagg agtgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt
    11641 tttcaatcgt gctctggcac gtcagaatga gaatcataag gccggtaata aatacatccc
    11701 ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa cgcaatggct
    11761 taaagattct ccctcacagc cattgcaaca gtcactgaaa gaccttgagc gggcttacaa
    11821 aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggtgac agaatgatgc
    11881 attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt ttctgccgaa
    11941 actgggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga aaaatgtcac
    12001 tgtcagccag tcctgcggta cgtggtacat cagtattcag acagaaagtg aagtatccac
    12061 tccggctcac ccttcagcat caatggtcgg gctggatgct ggcgtggcta aactcgccac
    12121 gctgtcagat ggcacagtct ttgagcctgt aaacagtttt cagaaaaacc agaagacgct
    12181 ggcgagactt cagcgacagt taagccgcaa ggtcaaattc agcaacaact ggcagaagca
    12241 gaaacgcaaa atacagcgac tgcattcctg tatcgcaaat atccgcaggg actaccttca
    12301 caaagtcaca acggccgtca gcaaaaacca cgcaatgata gtcattgagg atttgaaggt
    12361 cagcaacatg tcaaagtcag cagcgggtac ggtcagccag ccggggcgca atgtccgggc
    12421 aaaatcaggt ttaaa
//
LOCUS       DAZPDE010000109        12221 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPDE010000109 DAZPDE010000000
VERSION     DAZPDE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.109"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHU0J_002787"
     CDS             complement(<1..494)
                     /locus_tag="ACHU0J_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1217396.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHU0J_002788"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="ACHU0J_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1217397.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHU0J_002789"
     CDS             complement(1437..1979)
                     /gene="fimA"
                     /locus_tag="ACHU0J_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1217398.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2460..3326
                     /gene="folD"
                     /locus_tag="ACHU0J_002790"
     CDS             2460..3326
                     /gene="folD"
                     /locus_tag="ACHU0J_002790"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1217399.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3328..3540
                     /gene="ybcJ"
                     /locus_tag="ACHU0J_002791"
     CDS             3328..3540
                     /gene="ybcJ"
                     /locus_tag="ACHU0J_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1217400.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3648..4157
                     /locus_tag="ACHU0J_002792"
     CDS             3648..4157
                     /locus_tag="ACHU0J_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1217401.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4193..5578)
                     /gene="cysS"
                     /locus_tag="ACHU0J_002793"
     CDS             complement(4193..5578)
                     /gene="cysS"
                     /locus_tag="ACHU0J_002793"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1217402.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5752..6246
                     /gene="ppiB"
                     /locus_tag="ACHU0J_002794"
     CDS             5752..6246
                     /gene="ppiB"
                     /locus_tag="ACHU0J_002794"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1217403.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6249..6971
                     /gene="lpxH"
                     /locus_tag="ACHU0J_002795"
     CDS             6249..6971
                     /gene="lpxH"
                     /locus_tag="ACHU0J_002795"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1217404.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7089..7598
                     /gene="purE"
                     /locus_tag="ACHU0J_002796"
     CDS             7089..7598
                     /gene="purE"
                     /locus_tag="ACHU0J_002796"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1217405.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7595..8662
                     /gene="purK"
                     /locus_tag="ACHU0J_002797"
     CDS             7595..8662
                     /gene="purK"
                     /locus_tag="ACHU0J_002797"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1217406.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8799..9692)
                     /locus_tag="ACHU0J_002798"
     CDS             complement(8799..9692)
                     /locus_tag="ACHU0J_002798"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1217407.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9689..10336)
                     /locus_tag="ACHU0J_002799"
                     /pseudo
     CDS             complement(9689..10336)
                     /locus_tag="ACHU0J_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10339..11571
                     /gene="allC"
                     /locus_tag="ACHU0J_002800"
     CDS             10339..11571
                     /gene="allC"
                     /locus_tag="ACHU0J_002800"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR1217408.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11582..>12221
                     /gene="allE"
                     /locus_tag="ACHU0J_002801"
     CDS             11582..>12221
                     /gene="allE"
                     /locus_tag="ACHU0J_002801"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR1217409.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3159 c         3118 g         2978 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataaggagt
     1261 ataaagaaat gattgctttg gcttcttaca acacatcatt cccttttcat ttaataaagc
     1321 ggcatgatac aaagtgaacg tggtttcgat ttacattgcc accataccgc ttcagacaac
     1381 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     1441 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     1501 gtgcctttat aacgtgcaga aaaatgaaga acgttggtcc cggggatcaa gttctggttg
     1561 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     1621 ccgacacccg ttgctgttgt tgtatttgta ctggatacaa tggccaataa attatcattg
     1681 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     1741 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     1801 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     1861 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     1921 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     1981 tatattcctt caatctcatt tatcgactcc acatccgtat ataaccgatt actttattta
     2041 agacactgat agtagtaaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg
     2101 cactctattc taaaaaatag agagccccgt tagatgaata cttccgcgca aaatatattc
     2161 accacaaata tagacctgaa gcggtaaatt accaggctga aaattctttt tatattgtca
     2221 ggtatttctt aaattatctt aatccttaaa caaggaaata aatcagttcc agatttacaa
     2281 cgccatcatg tacgaaaaat gaagctttca atctcagcga cctcagcgac ggtgcgccta
     2341 accttcgcaa gaggtcgctt cacgcgataa atctgaaacg aaacctgaca gcgcgccccg
     2401 cttctgacaa aatagacgca tccccttcga cctacgtaac agatggaatc ctctctctga
     2461 tggcagcaaa gattattgac ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc
     2521 aaaaagttca ggctcgtatt gcagccggac tgcgggcacc aggactggcc gttgtgctgg
     2581 tgggtagtaa ccctgcatcg caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag
     2641 tcgggttcgt ctcccgctct tatgacctcc cggaaaccac cagcgaagcg gagctgctgg
     2701 agcttatcga tgcgctgaat gccgacaaca ccatcgatgg cattctggtt caactgccgt
     2761 taccggcggg tattgataac gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg
     2821 acggtttcca tccttacaac gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct
     2881 gcaccccgcg cggtatcgtc acgctgcttg agcgttacaa cattgatacc ttcggcctca
     2941 acgccgtggt gattggcgca tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc
     3001 tcgcaggttg caccaccacc gtgactcacc gcttcactaa aaatctgcgt catcacgtag
     3061 aaaatgccga tctgttgatc gttgccgttg gcaagccagg ctttattccc ggtgactgga
     3121 tcaaagaagg cgcaattgtg attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg
     3181 tgggcgacgt cgtgtttgaa gacgcggcta aacgcgcctc atacattacg cctgttcccg
     3241 gcggcgttgg cccgatgacg gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg
     3301 aatatcatga tccacagggt gagtaacatg gcgacatttt ctttaggtaa acatccgcac
     3361 gttgagctgt gcgacttgct gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa
     3421 atcgcgattg ccgaaggcca ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc
     3481 aaaatcgtcg ccggtcagac agtgagtttt gcaggacaca gcgtacaggt tgttgcctga
     3541 tccctttccc ggcctgtatc aacaggccgg ttctcttttc agacttttgc cctctacgcc
     3601 ccgttcatcc atgctgatag catcgacatt ttgccgaagc gaccatcatg ccaaccgtta
     3661 ttacgcacgc cgccgttccc ctttgcattg gtttagggtt gggttcgaaa ggtctgttat
     3721 ttgccggaat catcctggcg atgctgcccg atgccgacgt attgtcgttt aaatttggcg
     3781 ttgcttacgg caatgttttt ggtcatcgcg gttttaccca ttcgctggtg cttgcgtttg
     3841 ttgtcccgct cttatgtgtg ctgattggac gtcgatggtt cagggctggg ctgattcgct
     3901 gctggctgtt tttaaccgtc tcgttgctat cgcacagctt actggattcg gtaaccactg
     3961 gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc
     4021 aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacagcgtac gggcatcagg
     4081 tgattatttc cgaattgatg tgggtgtggt tgccggggat gctactgatg ggaatgttgt
     4141 ggtggcgcag acgataaccg gatgcgaaaa ctcgcatccg gcaattgcgc aattacttac
     4201 gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt
     4261 cacgtgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa
     4321 ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg
     4381 cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca
     4441 ttgcattcgc cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa
     4501 acagcacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg
     4561 cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt
     4621 agaggcgctc cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcagctggc
     4681 tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca
     4741 catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca
     4801 ccattccgga gtgcatccag tagttcacat actgaccatc atgggcacag gtggactgcg
     4861 cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt
     4921 ggttacccag ctgcttgcag ttcatggccg aacattcaat gtgccagcca ggacgacccg
     4981 cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt
     5041 ccatccggtt gcgtttgtca tcaaccacgt caacgcgcgc gccagcctgc agctggtcga
     5101 gatcctgacg cgacagcacg ccataagttg gatcggtcgg gacgtcgaac atcacgtcgc
     5161 tgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt
     5221 ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa
     5281 aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt
     5341 cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt
     5401 tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt
     5461 gaccgatatg acagagatcg taaacggtga ttccacacac gtacatgccg acttccccgg
     5521 cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg
     5581 aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg
     5641 cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat
     5701 atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact
     5761 ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca
     5821 gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt
     5881 gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc
     5941 accaaagaac cgatcaaaaa cgaagccaac aacggcctga aaaatacccg tggtacgctg
     6001 gcaatggcac gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt
     6061 gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt
     6121 gctgaagtgg ttgacggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt
     6181 aacggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc
     6241 gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga
     6301 tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata
     6361 ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc
     6421 agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg
     6481 gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc
     6541 cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc
     6601 tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc
     6661 aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgcg
     6721 cgaacagcaa agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg
     6781 ctgtggtcag tgcgatggaa aaacatcagg tgcaatggct aatccacggg catacccatc
     6841 gcccggcggt gcatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactgggtg
     6901 cctggcatac ggaaggttcg atggtgaaag tcacggctga tgacgttgag ctgattcatt
     6961 ttccgtttta aaaaacccac aactttgctg atttcacagc cacgcaaccg ttttccttgc
     7021 tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt
     7081 taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa
     7141 gcgactgggc taccatgcag ttcgccgtcg aaatcttcga aatcctgaat gtcccgcacc
     7201 acgttgaagt ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagcg
     7261 ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgccag
     7321 gcatgattgc cgccaaaacg ctggtgccgg tgctgggcgt gccagtacag agcgccgcac
     7381 tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta
     7441 cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt actggcagca caaattctcg
     7501 cgactcacga taaagagcta caccagcgtc tgaatggctg gcgcaaagcc cagaccgacg
     7561 aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa
     7621 cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc
     7681 ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga
     7741 gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt
     7801 gaaccgcgat gtgttcccga ttattgctga ccgtctgact cagaaacagc ttttcgataa
     7861 gctccacctg ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt
     7921 gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg
     7981 cggtcaatgg agtttacgcg ccaatgaaac cgaacagtta ccggcagagt gttacggtga
     8041 atgtattgtc gagcagggca ttaacttctc tggtgaagta tcgctggttg gcgcgcgcgg
     8101 ctttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt
     8161 gcgcaccagc gtcgcttttc cgcaggccaa cgcacagcag caggcgcaag ccgaagagat
     8221 gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt
     8281 caccccgcaa ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg
     8341 gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct
     8401 gccgttaccg caaccagtag tgaataatcc gtcggtgatg atcaatctga ttggtagcga
     8461 tgtgaattat gactggctga aattgccgct ggtgcatctg cactggtacg acaaagaagt
     8521 ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac
     8581 tgcgacgctg gaagccttaa tcccgctgct gccgccagag tatgccagcg gcgtgatttg
     8641 ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc
     8701 cttatccggc ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc
     8761 atcaggcatg gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc
     8821 gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacta
     8881 cggcttctga cataaccact caccgccgtt accttcggcc ccatcgaacc atcggctttg
     8941 gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg cacgttgctg cggcgttccc
     9001 cagttttcat ataccgcatc agcatcggtg aggatcacca gtccatcggc attaatctgc
     9061 tcggcgagca acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct
     9121 tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac
     9181 aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc
     9241 aaatatttac catcacgttt catctgccag ccgtaagccg cttccagtgc ctcttgttct
     9301 tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc
     9361 gaaacctcaa tgcgcgtcag caccgtcgta acaggcggca tctgcggctg tgcgctcaag
     9421 ctctgcgcca gcatatagcc aatcatcccc tggctttccg ccaccagtac atccagcgga
     9481 tacggttcta cctctttcca cgccagattc tgtaacgcca gcagccccac ctgcggcccg
     9541 ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gtgccagcgc gggtacagca
     9601 ctggcgatat tgcgatattg attttccgcc gtcagcgcct caccgcgctg gagtaaggcg
     9661 ttgcccccaa gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa
     9721 gccagaaccc cagcagcgta tctgcccctg acgtatgccc cagcgccagc agcgaatcaa
     9781 tcgcaacagc ggtacgtttc gggcaactca gagcatggac aaagtgcagg agtggcgagg
     9841 cgaaatatcc ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt
     9901 gaagattgtc ggaacaggca aaaaacgggc gaccggcgcg cgcatctaaa gcgccataat
     9961 accaggccgc cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc
    10021 ctttacccag ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt
    10081 gacgaaactg gcgtaattca gcgggcagcg gatcgcttgc cgccatcgcc agtggcccga
    10141 aaagtccggt ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgaag
    10201 gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc
    10261 gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat
    10321 cgaattgcgt gcggcgaaat tacacattcc cgtcaagcta tagaagaaac gctgccctgg
    10381 ctttcctctt ttggcgctga cccaacgggt gggatgaccc gtttacttta ttcgccggaa
    10441 tggctggaaa cccagcagca atttaaaaaa agaatggcag caagtgggct ggaaacacgt
    10501 ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc acaggaagtg
    10561 gttctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga cgggcaattc
    10621 ggcgcgctgg cggcgtggct ggcaattgac tggttgaaaa cgcaatacgg cgcaccgtta
    10681 cgtacggtcg aagtggtggc gatggcagaa gaagaaggca gccgcttccc gtatgtcttc
    10741 tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa tatctgtgat
    10801 gccaaaggaa atagttttgc cgatgcgatg aaggcttgcg gatttactct gccgaacgcc
    10861 ccactaactc cgcgtcaggc tataaaagcc tttgttgaac tgcatattga acagggctgt
    10921 gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg gcagcgtcgc
    10981 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
    11041 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
    11101 atgggcgatc cgctggttct gacctttggc aaagtagagc cgcgcccgaa tacggtaaat
    11161 gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg
    11221 cgcgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
    11281 ggtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggagctggtc
    11341 gccaccctga cagaattgtg tgaaagagaa aaactgaatt accgggtgat gcacagtggt
    11401 gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc
    11461 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattactga ccttgccgaa
    11521 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcaggaata aggagtcata
    11581 aatgggatat ttaaataacg tcaccggtta ccgcgaagat ttactggcta accgtgcgat
    11641 tgttaaacac ggtaatttcg cactgttaac cccggatggt ctgataaaaa atattattcc
    11701 ggactttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
    11761 tgattatctg gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg
    11821 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
    11881 atttgcctta agcgaaggtg gctatcttta ttgcccacca ggctccttaa tgacgtttgt
    11941 taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg ttccggtaga
    12001 aggccatgca ccgtggctgt tttctggcaa tgccagcgaa ctggaacgca ttcactatga
    12061 aggtatggat gatgtgatcc tgctggattt tctgcccaaa gagttaggtt ttgatatgaa
    12121 catgcatatc ctctcttttg caccaggtgc cagccacggt tatatcgaaa cacacgttca
    12181 ggaacatggt gcctatattc tttccggtca gggggtttat a
//
LOCUS       DAZPDE010000110        12155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPDE010000110 DAZPDE010000000
VERSION     DAZPDE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.110"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHU0J_002802"
     CDS             126..995
                     /locus_tag="ACHU0J_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1217410.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHU0J_002803"
     CDS             complement(1198..1551)
                     /locus_tag="ACHU0J_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1217411.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHU0J_002804"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHU0J_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1217412.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHU0J_002805"
     CDS             complement(3379..3672)
                     /locus_tag="ACHU0J_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1217413.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHU0J_002806"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHU0J_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1217414.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHU0J_002807"
     CDS             complement(5220..5696)
                     /locus_tag="ACHU0J_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1217415.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHU0J_002808"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHU0J_002808"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1217416.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHU0J_002809"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHU0J_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1217417.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHU0J_002810"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHU0J_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1217418.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHU0J_002811"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHU0J_002811"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1217419.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHU0J_002812"
     CDS             11052..11627
                     /locus_tag="ACHU0J_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1217420.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHU0J_002813"
     tRNA            11734..11809
                     /locus_tag="ACHU0J_002813"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3141 c         2980 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caatc
//
LOCUS       DAZPDE010000111        12131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPDE010000111 DAZPDE010000000
VERSION     DAZPDE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.111"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHU0J_002814"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACHU0J_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1217421.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="ACHU0J_002815"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="ACHU0J_002815"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1217422.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="ACHU0J_002816"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="ACHU0J_002816"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1217423.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="ACHU0J_002817"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="ACHU0J_002817"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1217424.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="ACHU0J_002818"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="ACHU0J_002818"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1217425.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="ACHU0J_002819"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="ACHU0J_002819"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1217426.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="ACHU0J_002820"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="ACHU0J_002820"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1217427.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="ACHU0J_002821"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="ACHU0J_002821"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1217428.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHU0J_002822"
     CDS             7140..7751
                     /gene="hisIE"
                     /locus_tag="ACHU0J_002822"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1217429.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHU0J_002823"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACHU0J_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1217430.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHU0J_002824"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACHU0J_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHU0J_002825"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACHU0J_002825"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1217431.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..>12131)
                     /locus_tag="ACHU0J_002826"
     CDS             complement(11945..>12131)
                     /locus_tag="ACHU0J_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217432.1"
                     /translation="WTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
BASE COUNT         3046 a         3081 c         3059 g         2945 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca a
//
LOCUS       DAZPDE010000112        12113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPDE010000112 DAZPDE010000000
VERSION     DAZPDE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.112"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..370
                     /locus_tag="ACHU0J_002827"
     CDS             <1..370
                     /locus_tag="ACHU0J_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217433.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHU0J_002828"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHU0J_002828"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1217434.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHU0J_002829"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHU0J_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1217435.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHU0J_002830"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHU0J_002830"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1217436.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHU0J_002831"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHU0J_002831"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1217437.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHU0J_002832"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHU0J_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1217438.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHU0J_002833"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHU0J_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1217439.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHU0J_002834"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHU0J_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1217440.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHU0J_002835"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHU0J_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1217441.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHU0J_002836"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHU0J_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1217442.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHU0J_002837"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHU0J_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1217443.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHU0J_002838"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHU0J_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1217444.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="ACHU0J_002839"
     CDS             complement(11912..>12113)
                     /locus_tag="ACHU0J_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1217445.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2933 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tatccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAZPDE010000113        12103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPDE010000113 DAZPDE010000000
VERSION     DAZPDE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.113"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            258..1028
                     /locus_tag="ACHU0J_002840"
     CDS             258..1028
                     /locus_tag="ACHU0J_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1217446.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1019..1492
                     /locus_tag="ACHU0J_002841"
     CDS             1019..1492
                     /locus_tag="ACHU0J_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1217447.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1599..2138
                     /gene="dnaT"
                     /locus_tag="ACHU0J_002842"
     CDS             1599..2138
                     /gene="dnaT"
                     /locus_tag="ACHU0J_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1217448.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2141..2878
                     /gene="dnaC"
                     /locus_tag="ACHU0J_002843"
     CDS             2141..2878
                     /gene="dnaC"
                     /locus_tag="ACHU0J_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1217449.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2927..3421
                     /gene="yjjA"
                     /locus_tag="ACHU0J_002844"
     CDS             2927..3421
                     /gene="yjjA"
                     /locus_tag="ACHU0J_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1217450.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3661..5952
                     /gene="opgB"
                     /locus_tag="ACHU0J_002845"
     CDS             3661..5952
                     /gene="opgB"
                     /locus_tag="ACHU0J_002845"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1217451.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHU0J_002846"
                     /pseudo
     CDS             complement(6090..7112)
                     /gene="lgoD"
                     /locus_tag="ACHU0J_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7251..8165
                     /locus_tag="ACHU0J_002847"
     CDS             7251..8165
                     /locus_tag="ACHU0J_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1217452.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8380..9741
                     /locus_tag="ACHU0J_002848"
     CDS             8380..9741
                     /locus_tag="ACHU0J_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217453.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9790..11454)
                     /gene="tsr"
                     /locus_tag="ACHU0J_002849"
     CDS             complement(9790..11454)
                     /gene="tsr"
                     /locus_tag="ACHU0J_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR1217454.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11573..12019)
                     /gene="hpaR"
                     /locus_tag="ACHU0J_002850"
     CDS             complement(11573..12019)
                     /gene="hpaR"
                     /locus_tag="ACHU0J_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1217455.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2956 a         3199 c         2932 g         3016 t
ORIGIN      
        1 actaaaaaac gcgcccagct atctattatc ctgacccgta aaggctaaac aataaattca
       61 ttatagtgat aatattcatg tgatagtttg tttgcggcga gagataattc gctttttatc
      121 accgagcttg tcccaataac caggattaat cagcgcaatt ttgatcttaa cctaccgcaa
      181 aacaaccgtg cttcatgata caaatggatt ctcattatct gaacaatacg cagcacgtct
      241 atgacaaagg gcgagttatg caaactgagc aacagcgagc cgtaacgcgg ctttgtatcc
      301 agtgtggatt atttcttttg caacatggtg cggaaagcgc gttggttgat gagctttcct
      361 cacgactggg tcgggcgctg ggaatggaca gcgtcgaaag ttctatctct tcgaacgcca
      421 tagtgctgac aactattaaa gatgggcaat gcctgacatc gacacgtaaa aatcacgatc
      481 gcggcattaa tatgcatgtg gtgactgaag tccagcacat tgtgattctt gcggagcatc
      541 atctgctgga ttacaaaggc gtagagaaac gatttagcca aattcaacca ttacgttacc
      601 caagatggct ggtagcctta atggttggcc tttcttgcgc ctgtttctgt aaactcaata
      661 acggtggctg ggatggtgcc gtcatcacct tctttgccag tacgaccgcg atgtatatcc
      721 gccagctgct ggcacaacgt catcttcatc cacagatcaa cttttgcctt accgctttcg
      781 ccgccaccac catttccgga ttgcttttgc aactccccac tttcagcaat acccccacca
      841 ttgcgatggc cgccagcgtt ctgctgctag tgccgggctt tccgttgatt aatgccgtcg
      901 ccgatatgtt taaaggccac atcaataccg gactggcacg ctgggcgatc gccagtctgc
      961 tgacactggc tacctgcgtc ggcgtagtga tggcactgac gatttggggg ctacgcggat
     1021 gggtgtgatc gaatttctgt tagcgttggc gcaggatatg atcctcgccg ccattcctgc
     1081 ggtcggcttt gcgatggtgt tcaacgttcc cgtgcgggcg ttacgctggt gtgcgctgct
     1141 tggctcgata ggtcatggtt cccgaatgat cttgatgacc agcgggttga atattgagtg
     1201 gtcaaccttt atggcttcta tgctggtcgg taccattggt attcaatggt cgcgctggta
     1261 tctggcgcat ccgaaagtgt ttaccgtggc ggccgttatc cctatgttcc cgggcatatc
     1321 ggcttatacc gcaatgattt cgtcggtaaa aatcagccag ttaggttaca gcgaaccgtt
     1381 gatgattacc ctgttaacca actttcttac agcttcatcg attgttggtg cgttatccat
     1441 cggtctttcc attcctggat tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct
     1501 cgcttaagca cacggatgag agacagcctc ctctcctccg tgtgttacta taaaagttat
     1561 ctcccttctc gttcatcgtt ccatatttga gaaacagtat gtcttccaga gttttgaccc
     1621 cggacgtcgt tggtattgac gccctggtac acgatcacca aaccgttctg gcaaaagctg
     1681 aaggcggtgt ggttgccgta tttgctaaca atgccccggc gttttatgcc gtcacgcctg
     1741 cacgcctggc tgaactgctg gcgctggaag aaaagctagc gcgtccggga agcgatgtcg
     1801 ctctggacga tcaactctat caggaaccgc aagccgctcc cgttgctgta cccatgggga
     1861 aattcgccat gtatccggac tggcaacccg atgccgattt tatccgcctg gcggcgctat
     1921 ggggcgtggc gctaagagag ccggtgacca ccgaagaact ggcctcattc attgcctact
     1981 ggcaggcgga aggtaaagtc tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc
     2041 tgcaaatcgg tcgtgccagc aacggcggac tgccgaaacg agatgtgaat acggtcagtg
     2101 aacctgacag ccaaattcca ccaggattca gagggtaacg atgaaaaacg ttggcgacct
     2161 gatgcaacgc ctgcaaaaaa tgatgcctgc ccatatcaaa cctgcattca aaacgggtga
     2221 agagctactg gcctggcaga aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga
     2281 aaatcgggcg atgaaaatgc agcgcacctt taaccgctcc ggtattcgtc cactgcatca
     2341 gaactgctcc tttgagaact atcgcgttga gtgtgaaggg cagatgaatg cgttaagcaa
     2401 agcgcgccag tatgtcgaag agtttgacgg caacatcgcc agcttcatct tttctggtaa
     2461 gccaggaacc ggcaaaaacc atctggcggc ggcaatctgc aacgagctgc tgctacgcgg
     2521 taaatccgta ttgatcatca ccgtggccga tattatgtcg gcgatgaaag ataccttcag
     2581 gaatagcggt accagcgaag aacaactgct taacgatctg agcaacgttg atctgctggt
     2641 gatcgatgag atcggcgtgc agaccgaatc gaaatacgaa aaagtgatca tcaaccagat
     2701 cgtcgatcgc cgctcttctt ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga
     2761 agagatgacc aagttgctgg gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt
     2821 atgggtgatc ttcaactggg atagctaccg tagccgggta acgggtaaag agtattaatc
     2881 cccgtttctg gcggtatact gcgacacaat tccaccttga gtaatgatga tgaaaactat
     2941 taaacatctt ctgtgctgtg ccattgccgc cagcgcatta atttccaccg gggtgcatgc
     3001 tgcgtcctgg aaagatgcgc tctccagcgc cgccagcgaa cttggcaacc aaaacagcac
     3061 gacacaggaa ggcggttggt cgctcgcgtc attaactaac ttgcttagca gcggaaacca
     3121 agccttaagc gcagataaca tgaacaatgc cgcaggcatt ctgcaatact gcgcgaagca
     3181 aaagctggct tcggtaaccg atgccgaaaa catcaagaac caggtgctgg aaaagctggg
     3241 cctgaacagt gaagagcaaa aagaagacac caactatctg gacggtattc agggtttgct
     3301 gaaaacaaaa gatggtcagc aactcaatct ggataacatc ggaacgactc cgctggcaga
     3361 aaaggtgaaa accaaagcct gcgatctggt gttaaagcag gggctgaact tcatttcctg
     3421 aaccgcgaaa tgttacccgg ctgacgcggc tctctcctgc ccttttccct gctcctggac
     3481 ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt ataaactaat
     3541 tcctaacaac atcgcatttt tttgacacta gaattgcagc aatatggcga cgccattaca
     3601 ttgtgcttct caaaaagctt aaacgttggc taaacggccc ggtttagtga ggatcgccca
     3661 ttgtcagaac tactctcttt cgcccttttt ctcgcctctg tgctgattta cgcatggaaa
     3721 gcgggacgta acacctggtg gtttgcagcc acgttaacgg tgctggggct atttgtcgtt
     3781 ttaaatatca ccctgtttgc cagcgactat tttactggcg atggtattaa cgacgcggtt
     3841 ctctatacct taaccaacag cctgaccggt gctggcgtca gcaaatacat cctgccgggt
     3901 atcggcattg tgctggggct gacggcggtg ttcggtgcgc tgggctggat cctgcgccgt
     3961 cgtcgtcatc atccgcacca ttttggttac agcctgctgg cgctcttact ggcgctgggt
     4021 tcggtggacg ccagcccggc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc
     4081 gatggcgacc cggactttgc ggcttattat aaagagccgt cgaaaactat ccctgacccg
     4141 aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgaa
     4201 gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc
     4261 cacacgcagc agctgccagg aacggattac acgattgcgg gcatggtggc ttctcagtgc
     4321 ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg cctctgtctc cagcttcttc
     4381 ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg
     4441 cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc tgaaatcgca cggcttcgac
     4501 cacttatacg gctcagaaga gctgaaaagc gtggtagccg acccgcacta tcgcaacgac
     4561 tggggattct acgacgatac cgttctcgat gaagcgtgga aaaagtttga agagctttcc
     4621 cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt
     4681 tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca
     4741 ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt ttatcaacaa aatcaaagcg
     4801 tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg accatttagc gatgaacaac
     4861 acggcgtgga aatacctcaa taagcaggat cgcaataacc tgttttttgt cattcgtggc
     4921 gacaagccgc agcaaaagac gctggcagtg aagcgtaaca cgatggataa cggcgcgacg
     4981 gtgctggaca ttctcggtgg cgataactat ctcggacttg gtcgtagcag tttatccggg
     5041 cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccagat
     5101 atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa
     5161 aacatgattg ccttctcggg tagccatttc cgtttgccgc tgctgttgcg ggtttcagac
     5221 aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac cgctgcgttt ccagctggcc
     5281 gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt gctacaagat ggcacaactc
     5341 tgggctccgg aactggcact ttccaccgac tggtgtgtct cgcaggggca gcttggcggt
     5401 cagcaaattg ttcagcatgt tgacaaaaca acatggaaga gcaagacggc atttaaagat
     5461 acggtcatcg acatggcgcg ttacaaaggc aatgtcgata cgctgaagat tgttgataac
     5521 gatattcgct acaaagccga cagtttcatc tttaacgtcg ccggtgcgcc agaagaggtg
     5581 aaacagttta gcgggatttc ccgtccggag tcgtggggcc gctggtccaa cgcgcagctg
     5641 ggcgatgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt
     5701 accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa
     5761 gaacaaactc ttgtgctggg caatgaagtg accaccacca cactgcattt cgataaccca
     5821 accgatgccg acacactggt aattgtgccg ccggaacctg tctcaaccaa cgaagggaat
     5881 atcctcggac actcgccgcg taagctcggg atcggcatgg tggaaattaa agtggtagaa
     5941 cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg gtgcgaccta cacgccttct
     6001 ccggcttaca acacattatt aagaaacaaa aaaaacagcg cccaacactt ccccggacgc
     6061 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt aatcaactca
     6121 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg
     6181 gttaacatca tgtcggcagt gattttccct tccgccatca gtcgaccgac tttagcgaaa
     6241 tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt cttttttatg gaattccgga
     6301 tcggagaact gcaactcacc tttaaacaga ccgacaaata ccaccgtgcc gccgtgacga
     6361 atcagattca cggtattatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6421 gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgacgggtcc
     6481 agcacgggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6541 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6601 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6661 ctaatagcga aaggttcaat caatgcggcc gcctgcgggt caataccgtc tgcgggcaaa
     6721 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6781 actgaaattt tttcacagca gttggtacgc ccgcttttac acgccgggca ttgctgacag
     6841 gcaacataag ggatcacagc aacttgctga ccatttttaa gatcagcaat atttttaccc
     6901 agcccaacaa tctccccaca tatttcatgg cctaaaaccc gtggataact aaaaaatggt
     6961 tgatttccac cctaggcatg aatatcggta ccgcaaatcc cgacagactt aatttttatt
     7021 aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa ttctttcggc
     7081 tgctggcaaa ttaaaacatt catcgtagac atgattcggc tatccatttt ttgttgcctt
     7141 agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt gaaaacacac
     7201 gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat atgagtcgtt
     7261 cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg cggggtcata
     7321 tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc agccgcacta
     7381 cagtgcgtca tattctcagc caattacgcg aatgcggcgt cttaacgcag gttggaaacg
     7441 actatgttat tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa
     7501 tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgtcagt
     7561 tacgccctag ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg
     7621 tggttgtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc
     7681 gcggccagtg gagcatgaag caattcgatc aatcctacgc cgagcagctg tttgagctac
     7741 gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc
     7801 gctggctgca ggcgaaaacc atgctagaac gtcatcggct actgcgtgac aacatcggca
     7861 atagctttcg catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg
     7921 ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt cattttcact
     7981 atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gaacatatga
     8041 ccattctcag cgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc
     8101 atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc
     8161 attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa
     8221 ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga
     8281 gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg taatgcacaa
     8341 ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc
     8401 ctcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat
     8461 ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta
     8521 ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc aaatttaacg
     8581 attcgtgaag aattgggatt aagtgccacc gaaatcggcg ctttgctctc cgtgttttca
     8641 ctcgcttacg gcattgcgca acttccttgc ggcccactat tggatcgtaa aggcccacgc
     8701 ctgatgctgg gactggggat gttcttctgg tcactgttcc aggccatgtc tggcatggtg
     8761 cacagcttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga agcgccgatg
     8821 aacccatgcg gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg cggacgcccg
     8881 atgggcttct tcaacgcagc ttctaccatt ggcgttgctg taagcccacc gattctggcg
     8941 gcaatgatgc tggtgatggg ctggcgcggg atgtttatta ccattggtgt actggggatt
     9001 tttctcgcca tcggctggta tatgctctat cgcaaccgcg agcacgtaga actgactgcc
     9061 gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc gccgagatcc gctcaatttt
     9121 gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgct cggattcagt
     9181 ggcatcaact acactgcgtg gctgtatctg gcctggcttc ctggttacct gcaaacagcc
     9241 tataacctgg atttaaaaag cacagggttg atggcggcta tccctttcct gtttggggct
     9301 gccgggatgc tggtcaacgg ttacgttact gactggctgg tcaaaggggg aatggctccg
     9361 attaaaagcc gtaagatctg cattattacc gggatgttct gttctgccgc ctttacgctg
     9421 atagtaccac aagcgacaac atccatgatg gcggttctgc tgattggcat ggcactgttc
     9481 tgtattcact ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc
     9541 atgactgcgt cggtgggcag tatccagaac tttgccagct tcatctgcgc ctctttcgcg
     9601 ccgatcatta ctggttttat tgttgataca acccactcat tccgtctggc actaatcatt
     9661 tgcggttgcg tcaccgcagc gggagcactg gcgtacatct tcctggttcg ccagccgatc
     9721 aacgacccgc gtaaagatta aactccctat gcccgataac attttgctta tcgggcattt
     9781 ttatggcgat taaaacgttt cccagttctc cccgctatct gccactgcca ttttacgcgg
     9841 cgtagctggc gtcacggttt ttaccacagc cgatgtttca cgctgctgtt gctgctgttg
     9901 ctgaatccgg aacactgcca cagcttcggt caggcgactg gcctgctctt ccagcgcggc
     9961 ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt tgagttaccc ggtccatctc
    10021 agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagctat
    10081 ttcgcccata atgtccgtca cgcgggtcac agcactgaca atctccgcca ttgtttcccc
    10141 ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct
    10201 tttaatttca cgaaccgcct gggcgctacg ctgggccaga ttacgcactt ctcccgcaac
    10261 caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct tcaaccgccg cgttcaaagc
    10321 cagaatattg gtctggaagg taatgccgtc aattacgctg ataatatcgg cgattttctg
    10381 cgaactggtg gagatatcgc gcatagtctg caccacgtta tctaccactt tgccgccgcg
    10441 ttgcgccgtt tcagaagcac ttaatgccag atggctggcc tggcgcgcat tctcggcgtt
    10501 ctgtttcacc gttgcggtca gttgctccat gctggctgcc gtctcttcca gcgaagcggc
    10561 ctgttgctcg gtgcgcgaag agagatcatt attgccggta gcgatttcgc tggcaccgct
    10621 atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat
    10681 atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt
    10741 caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgctac
    10801 cagcgaggct ttaataccga accagacggc gaagatgacc gccagtacga cgatcatcac
    10861 gcccaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc
    10921 atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata
    10981 tccctgggtc ggctgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag
    11041 ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgccg ctgcggcggt
    11101 gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt
    11161 cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt
    11221 ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc
    11281 gacccagctg ccattcagcg tgggttgctg ctggcgaatg gtttgtaaaa cagtgaaatt
    11341 ttctttgtca ttctttaagg cattaaaaaa cagaccgcct gatgtcagtt gtaaaaggcc
    11401 aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc
    11461 tctttcctgt ggaccgatgc aagattttcg gcctggaaag caaaaacttt atgaaaaggt
    11521 gtaacccgcc atccatggaa aaaccaggcg ctggttatca gatactaaaa agttattcat
    11581 tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc aataaattcc tctaacagat
    11641 gggttaactg ttgcattttc tcggcagtaa actgagcttc aatctgccga taggcttctt
    11701 caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc tttggtcagc gagatataga
    11761 gcttccgctg atcattgatc ggctttaatc gcaacactaa accgtcgcgt tccatccgcg
    11821 tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga tcgtgaaaat
    11881 ccattgatgg gctttccgcc agaatacgca cgatgcgcca ctgttgctca gtcaaattat
    11941 gccgtttaac gattgggcga aaataactca tcgccgcttc tcgcgcctgg agcaacgcaa
    12001 tggttagtga gtcgtgcatt atctttcccc tgaaaaattc aaaatcatta atatagaaac
    12061 aattaatcat cacgttaccg ggactgagct gcccccgcaa ttc
//
LOCUS       DAZPDE010000114        11990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPDE010000114 DAZPDE010000000
VERSION     DAZPDE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.114"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHU0J_002851"
     CDS             complement(<1..2251)
                     /locus_tag="ACHU0J_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR1217456.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHU0J_002852"
     CDS             complement(2275..2898)
                     /locus_tag="ACHU0J_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR1217457.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHU0J_002853"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHU0J_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHU0J_002854"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHU0J_002854"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1217458.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHU0J_002855"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHU0J_002855"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1217459.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11919)
                     /gene="yfaL"
                     /locus_tag="ACHU0J_002856"
                     /pseudo
     CDS             complement(8356..11919)
                     /gene="yfaL"
                     /locus_tag="ACHU0J_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2777 a         3320 c         2955 g         2938 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc attgcccatc ggcgatagac tggccccctg aatctccaga
    11941 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact
//
LOCUS       DAZPDE010000115        11914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPDE010000115 DAZPDE010000000
VERSION     DAZPDE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.115"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(150..890)
                     /gene="cysQ"
                     /locus_tag="ACHU0J_002857"
     CDS             complement(150..890)
                     /gene="cysQ"
                     /locus_tag="ACHU0J_002857"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1217460.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1080..3023
                     /gene="cpdB"
                     /locus_tag="ACHU0J_002858"
     CDS             1080..3023
                     /gene="cpdB"
                     /locus_tag="ACHU0J_002858"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1217461.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3149..3529)
                     /locus_tag="ACHU0J_002859"
     CDS             complement(3149..3529)
                     /locus_tag="ACHU0J_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1217462.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3618..4478
                     /gene="qorB"
                     /locus_tag="ACHU0J_002860"
     CDS             3618..4478
                     /gene="qorB"
                     /locus_tag="ACHU0J_002860"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1217463.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4587..5552
                     /locus_tag="ACHU0J_002861"
     CDS             4587..5552
                     /locus_tag="ACHU0J_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1217464.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5660..6322
                     /gene="ytfE"
                     /locus_tag="ACHU0J_002862"
     CDS             5660..6322
                     /gene="ytfE"
                     /locus_tag="ACHU0J_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1217465.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6367..7779)
                     /gene="cycA"
                     /locus_tag="ACHU0J_002863"
                     /gene_synonym="dagA"
     CDS             complement(6367..7779)
                     /gene="cycA"
                     /locus_tag="ACHU0J_002863"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1217466.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8087..8707)
                     /gene="fklB"
                     /locus_tag="ACHU0J_002864"
     CDS             complement(8087..8707)
                     /gene="fklB"
                     /locus_tag="ACHU0J_002864"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1217467.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8926..9471
                     /locus_tag="ACHU0J_002865"
     CDS             8926..9471
                     /locus_tag="ACHU0J_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR1217468.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9455..10102)
                     /locus_tag="ACHU0J_002866"
     CDS             complement(9455..10102)
                     /locus_tag="ACHU0J_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1217469.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10314..11105
                     /locus_tag="ACHU0J_002867"
     CDS             10314..11105
                     /locus_tag="ACHU0J_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR1217470.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSNRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11115..>11914
                     /locus_tag="ACHU0J_002868"
     CDS             11115..>11914
                     /locus_tag="ACHU0J_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1217471.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         3018 a         3087 c         2992 g         2817 t
ORIGIN      
        1 actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt tttgcacttt
       61 aaatacaatc tctttttatt tataactttt taggttggga taaatcagga tggcagaatc
      121 aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa ccccggattc
      181 aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct
      241 ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt tggtccgaag
      301 cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg
      361 gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg
      421 gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca
      481 ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc
      541 gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat
      601 tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc
      661 tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac cggaatatcc
      721 ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc cgctgccgtt
      781 accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc
      841 ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct
      901 cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc
      961 caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat
     1021 aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga
     1081 tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg aatgcagcga
     1141 cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt
     1201 attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg
     1261 atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga
     1321 gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt
     1381 ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact
     1441 acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca
     1501 acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg
     1561 aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc
     1621 cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata
     1681 ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg
     1741 tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag
     1801 tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg
     1861 ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa ggcacgctga
     1921 atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac
     1981 aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga
     2041 tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac
     2101 tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg
     2161 acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca
     2221 acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc
     2281 cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct
     2341 atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc
     2401 ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct
     2461 ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca ctcatcaact
     2521 gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg
     2581 ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga
     2641 ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctggttgcca
     2701 ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt
     2761 ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac
     2821 gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg atagctgccg
     2881 ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcgtttatta
     2941 aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt
     3001 atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca accgcatccg
     3061 gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc atgaaccgta
     3121 gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca
     3181 cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct
     3241 cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac gcgatacggt
     3301 taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt tcactcaccc
     3361 caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa
     3421 tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg
     3481 caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt tcatacttac
     3541 ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa
     3601 aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata
     3661 ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc
     3721 cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct gactacagcg
     3781 atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca
     3841 gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg
     3901 gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg
     3961 ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc
     4021 tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg
     4081 gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg gcagattatg
     4141 cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg
     4201 cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca
     4261 aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg
     4321 gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg
     4381 gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg acaacgttag
     4441 ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc
     4501 gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc
     4561 ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc ctgttagcag
     4621 ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt
     4681 tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt gcctggctgg
     4741 gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg atgctcacta
     4801 tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt actggcgcgc
     4861 ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc
     4921 tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca ctgatttgta
     4981 ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc cccgattttg
     5041 actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat
     5101 ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt
     5161 gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat ctcgtcgcct
     5221 gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc cgtccgctgg
     5281 tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc gcactctgct
     5341 ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt gttttcgaaa
     5401 cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg ctaatgacgc
     5461 tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa
     5521 aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta aataagcctc
     5581 tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat cttatcttat
     5641 taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac tggcgctctc
     5701 tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct gtggcggtaa
     5761 gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca ttgaagctga
     5821 actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc cgctggcaga
     5881 aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc tgccggagct
     5941 gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg
     6001 gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga tgaaagaaga
     6061 gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg ggccaatcag
     6121 cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca aacacactac
     6181 caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt ataacggcat
     6241 taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg tactgttccc
     6301 acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc cagcatgata
     6361 atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc agcccaaccc
     6421 cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt
     6481 cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca gcttgccgag
     6541 cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt acaccaggta
     6601 agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg ttgtaatcat
     6661 cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc cgagcagaca
     6721 gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc
     6781 tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag agaagacacc
     6841 gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc tggcagccgc
     6901 aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga ctaccgaact
     6961 ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa tgatacggat
     7021 cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt ccgcagctgt
     7081 tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa
     7141 gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga atgacgcttc
     7201 cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga caatcagcga
     7261 cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga acattttcac
     7321 ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga cccagtcgga
     7381 gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg ccataccggt
     7441 tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg gccccagtaa
     7501 atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca attcccccat
     7561 tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa tgatcgacgg
     7621 cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa tggcacctcc
     7681 aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact gttcagccgg
     7741 agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc ctgtacctgt
     7801 ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg
     7861 actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa
     7921 ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac
     7981 acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg
     8041 ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca
     8101 gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct gcgccgcgct
     8161 cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag
     8221 tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac
     8281 gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa
     8341 cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc
     8401 cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacgcctt
     8461 cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac
     8521 gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg
     8581 caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt
     8641 gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg
     8701 tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt
     8761 ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc
     8821 aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc gttagcttat
     8881 tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc cgggcgcttt
     8941 gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg cttcatggac
     9001 ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt ggtggtcgga
     9061 tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga agcgcagtta
     9121 gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac ccaaacgcaa
     9181 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
     9241 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
     9301 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
     9361 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
     9421 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
     9481 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
     9541 ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg atcaaataat
     9601 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct ggcttaaaaa
     9661 tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga gcatggttaa
     9721 tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt tcactatgca
     9781 caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca ataattttct
     9841 caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt tgtcgaatat
     9901 cgttccaggt atattccagc acttcgataa ataaattttc tttattacca aagtggtgat
     9961 aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg gccccttcat
    10021 aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc aggctctgta
    10081 cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg actatcataa
    10141 cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat caggttatca
    10201 aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt ttatcctcca
    10261 gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat tgtatgaaat
    10321 ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag ggctgtgcgc
    10381 aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat gccgacagcg
    10441 tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa gtgatttgct
    10501 tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg gcgcaaaacc
    10561 agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa aaacgcggtg
    10621 acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac acccgcgcgc
    10681 cgttcttcct tgctcaggca ttcagtaatc gcttactggc acagccgaaa ccggaagcgg
    10741 agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg ctggcgatga
    10801 atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt ctgtttgctg
    10861 ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc atcaaaactg
    10921 atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt ctggtgccgt
    10981 ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg gcggaaggta
    11041 aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt atgccgcgct
    11101 tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt atcaataccg
    11161 cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc gcacgcggta
    11221 ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag caaatcaccc
    11281 gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact tactacaccg
    11341 cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg ctggacgacg
    11401 cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca acgggcacta
    11461 aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg ctgccacatt
    11521 cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca gctgccgatc
    11581 gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc gatcccgatg
    11641 gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat ccggatctcg
    11701 ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc gactggttaa
    11761 tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc atcaatattc
    11821 cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa ctggaaatat
    11881 tctcgcctta ctggtggcag aaagatatta acag
//
LOCUS       DAZPDE010000116        11793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPDE010000116 DAZPDE010000000
VERSION     DAZPDE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.116"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHU0J_002869"
     CDS             38..778
                     /locus_tag="ACHU0J_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1217472.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHU0J_002870"
     CDS             837..1223
                     /locus_tag="ACHU0J_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1217473.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHU0J_002871"
     CDS             1232..1561
                     /locus_tag="ACHU0J_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1217474.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHU0J_002872"
     CDS             1533..4574
                     /locus_tag="ACHU0J_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1217475.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHU0J_002873"
     CDS             4574..4903
                     /locus_tag="ACHU0J_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1217476.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHU0J_002874"
     CDS             4903..5601
                     /locus_tag="ACHU0J_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1217477.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHU0J_002875"
     CDS             5606..6349
                     /locus_tag="ACHU0J_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1217478.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHU0J_002876"
     CDS             6313..6888
                     /locus_tag="ACHU0J_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1217479.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHU0J_002877"
     CDS             6949..10428
                     /locus_tag="ACHU0J_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1217480.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHU0J_002878"
     CDS             10496..11095
                     /locus_tag="ACHU0J_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1217481.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11793
                     /locus_tag="ACHU0J_002879"
     CDS             11159..>11793
                     /locus_tag="ACHU0J_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1217482.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2774 a         2795 c         3790 g         2434 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg ctaaccatct gtc
//
LOCUS       DAZPDE010000117        11690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPDE010000117 DAZPDE010000000
VERSION     DAZPDE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.117"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHU0J_002880"
     CDS             <1..851
                     /locus_tag="ACHU0J_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1217483.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHU0J_002881"
     CDS             873..2282
                     /locus_tag="ACHU0J_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217484.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHU0J_002882"
     CDS             2260..3039
                     /locus_tag="ACHU0J_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1217485.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHU0J_002883"
     CDS             3036..3896
                     /locus_tag="ACHU0J_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1217486.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHU0J_002884"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHU0J_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHU0J_002885"
     CDS             complement(4636..4896)
                     /locus_tag="ACHU0J_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1217487.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHU0J_002886"
     CDS             complement(4887..6158)
                     /locus_tag="ACHU0J_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1217488.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHU0J_002887"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHU0J_002887"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1217489.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHU0J_002888"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHU0J_002888"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1217490.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHU0J_002889"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHU0J_002889"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1217491.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHU0J_002890"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHU0J_002890"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1217492.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHU0J_002891"
     CDS             11500..11652
                     /locus_tag="ACHU0J_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1217493.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2809 a         2982 c         3230 g         2669 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg
//
LOCUS       DAZPDE010000118        11682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPDE010000118 DAZPDE010000000
VERSION     DAZPDE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.118"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            47..610
                     /locus_tag="ACHU0J_002892"
                     /pseudo
     CDS             47..610
                     /locus_tag="ACHU0J_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHU0J_002893"
     CDS             complement(681..1130)
                     /gene="rplI"
                     /locus_tag="ACHU0J_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1217494.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHU0J_002894"
     CDS             complement(1172..1399)
                     /gene="rpsR"
                     /locus_tag="ACHU0J_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1217495.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHU0J_002895"
     CDS             complement(1404..1718)
                     /gene="priB"
                     /locus_tag="ACHU0J_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1217496.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHU0J_002896"
     CDS             complement(1725..2120)
                     /gene="rpsF"
                     /locus_tag="ACHU0J_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1217497.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHU0J_002897"
     CDS             2446..2721
                     /gene="yjfY"
                     /locus_tag="ACHU0J_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1217498.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHU0J_002898"
     CDS             complement(2850..3536)
                     /gene="ulaF"
                     /locus_tag="ACHU0J_002898"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1217499.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHU0J_002899"
     CDS             complement(3536..4390)
                     /gene="ulaE"
                     /locus_tag="ACHU0J_002899"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1217500.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHU0J_002900"
     CDS             complement(4400..5050)
                     /gene="ulaD"
                     /locus_tag="ACHU0J_002900"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1217501.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHU0J_002901"
     CDS             complement(5064..5528)
                     /gene="ulaC"
                     /locus_tag="ACHU0J_002901"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1217502.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHU0J_002902"
     CDS             complement(5538..5843)
                     /gene="ulaB"
                     /locus_tag="ACHU0J_002902"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1217503.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHU0J_002903"
     CDS             complement(5859..7256)
                     /gene="ulaA"
                     /locus_tag="ACHU0J_002903"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR1217504.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHU0J_002904"
     CDS             7611..8675
                     /gene="ulaG"
                     /locus_tag="ACHU0J_002904"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1217505.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHU0J_002905"
     CDS             8783..9538
                     /gene="ulaR"
                     /locus_tag="ACHU0J_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1217506.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHU0J_002906"
     CDS             complement(9535..10284)
                     /gene="yjfP"
                     /locus_tag="ACHU0J_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1217507.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHU0J_002907"
     CDS             10466..10795
                     /gene="bsmA"
                     /locus_tag="ACHU0J_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1217508.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHU0J_002908"
     CDS             10944..11219
                     /gene="yjfN"
                     /locus_tag="ACHU0J_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1217509.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11336..>11682)
                     /locus_tag="ACHU0J_002909"
     CDS             complement(11336..>11682)
                     /locus_tag="ACHU0J_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1217510.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
BASE COUNT         2959 a         3087 c         2870 g         2766 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtaa caatgaatgg
       61 tgataattct tttggatgga accatgaagc tacattagat aatattcacg cccagaaata
      121 caataaagct tacgcgggtg cgaggttaac cgcagaactg aaatatttat tgcaagatgt
      181 cgaatccttc gaacccaaca gtaaatacac aatacacgag gttgttttgg gacctggtta
      241 tagtactcca gactataccg gccaaacgat aggatacgtt gtaacccttc ctgcccaaat
      301 gcctaattgc tgctcatctg aactaccaac aatcgatctt tatatcgacc aacttcacac
      361 tgttacaggc gtttctaatg ccctcggatt catcattgct gcattgctaa acgcgtattc
      421 tgatttaccc catgatttga aaattggtct acgttcctta tcaaattcag ccgcaatcta
      481 ctcgggttta ggtttcgagc gagtgccgca ggaccgagac ataaggagtg atcgtatgca
      541 tctaacgcca gcaaaccatc ctgacttatg gacacaagag aatggtgaat ggatctactt
      601 gcgaaattaa tgcctgacga aaaatagaaa gcaaaacgcc gaccaatggt cggcgttttt
      661 acgtctcgtt gaataacgaa ttattcagct actacgttta cgatcacttt cgcgaatact
      721 tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc
      781 agacgaactt cgctcttagc cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg
      841 cgagtaccga tggaaccgaa cagtttacct tcgtcgccag ctttagacgc gatggtaaca
      901 gtttccagtg cattgatttt ctcagcgcga gcattagcag ctgccagaac ttcagccagt
      961 ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt ggtagctgga
     1021 acagctttac cctgcggtac caggaagtta cgagcatagc ccgctttaac gtttacctga
     1081 tcacccaggc tacccaggtt tgctacttta tcaagcagaa taacttgcat taccttatcc
     1141 tctcaaagtc gtattaatgg accgtgaccg attactgatg gcgatcagtg tacggcagca
     1201 gggacaggta gcgagcgcgt ttgatagcgc gagccagctg acgctggtat tttgcacggg
     1261 taccggtgat acggcttggg acaatcttac cgctttcggt gatgtagttt ttcagcgtag
     1321 cgatatcttt atagtcgatc tcttgaacgc cttccgcggt gaaacggcag aacttgcgac
     1381 gacggaaata acgtgccata tggctagtct ccagaatcta tcaattcaat ctgctcggca
     1441 tgcaaaacca ttttgctcag tccgttcttt gccttgtggc atgaaatgaa cccctgaacg
     1501 gttatgcgac tgccgaccgt tatactgtga gtaatggcct ggttttcgtg tccgctaaca
     1561 ataacgggca tttgacacca tgcctgccgg tgaaagccgg cttcctcctg cacagaacga
     1621 tgctcaagca cgaactggca gtgaggaatt cctgatggac tgacctttcg aaggggagtc
     1681 ctgcacacgg tgccggacaa caccagacgg ttggtcatca gaaattactc ttcagaatcc
     1741 ccagcttcag catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc acggcgctcg
     1801 tctttcgctt taaccatcgg agatgcttcg gtaacagcgt gcttggtacg cataaccatg
     1861 ctgcggataa cggcatcgtt gaagcggaag gtagtttcca gctcatcgat cacttcctgc
     1921 ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc
     1981 agctgacggc ggccccagtc ttccagacgg tggatcttgc cttctgcacc agtgatggca
     2041 gcagtgtagc gctcgatcat gcccggaacc tgttcgctct ggtcaggatg gaccataaaa
     2101 acgatttcgt aatgacgcat cgaattgctc cttacggatt attcagcctc ctgtctgggt
     2161 cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat
     2221 agtactggca taccccgcta aactcaaggg cggagcagaa ataaatctcc cctttcgcaa
     2281 ataacctcta aaccgctgat ttgaagttca tcacattccg tcacagtgtt ttttgaacaa
     2341 ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat
     2401 catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgagtt
     2461 ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat
     2521 aaccaccagg ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaatcat
     2581 aatttcgcca ctgaaagcga agcacgtcag gcattaaatg aagaaacaga tgcgcaaggc
     2641 gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc
     2701 agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt
     2761 tgcccccttc gcagggggct ttttttgaaa aacggctgcg gaattagacc agttatctcc
     2821 cgaggaagga aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttaggaccg
     2881 tgcttacgca tgaagtgttt attcatcagg tagctgtcga tatgattgag ttgtggatta
     2941 atactgcgcg caatccacgc cattttcgcc acttcttcca tcactaccgc gttatgcacc
     3001 gcgtcgtggg catctttccc ccaggcgaac ggtccgtgct gatacaccac aattcccggc
     3061 gtatgcagcg gctcggcgtt gcccagcgtt tcgataatca ctttgccagt gttcagttca
     3121 tactcaccct gcacctcttc ttcgcttaat ccacgcgtac acgggatatc accaaagaag
     3181 tagtcagcgt gcgtggtgcc taatgccggg atcgccagcc ccgcctgcgc ccatgcggtg
     3241 gcatgagtgg agtgggtatg gacaatgccg ccaagcgacg ggtaacgacg gtagagttcg
     3301 agatgcgtcg cagtgtcgga agatgggcga tactcccctt ccaccacctt gccgctcata
     3361 tcgaccacca ccatatcgtc tgctttcatg gtttcgtagg caacgccgct gggcttgatc
     3421 accaccagcc cgcgttcgcg gtcgatagcg ctgacgttgc cccaagtaaa ggtcaccagc
     3481 ccatagcgcg gcagatccat gttggcttca aacacctgct gttttagctt ttgcattatg
     3541 ccgcctccac catgcctgct ttggccatgc gcgctttcac ccaatcacac gctttcacca
     3601 cttctgccgc cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat
     3661 agccactctg tttgagcgtt tcgaagcaac gttcgaaatc caccacacct tcgccaaatg
     3721 gcacgttttt gaagacgccc ggtttggtgt ctttcacatg caccgcgacg atatgcccga
     3781 ttccggcctg caactccatc tgcacatcgt tgtcccacgc cgacaggttg ccgatatccg
     3841 ggtagagctg gaaccacggg ttgttgagat agtgcgcgta acccagcgcc ttgctgatgg
     3901 agttcatcaa cggataatcc atgatctcca tcgccagcgt cacctgcgcg cggctcgcca
     3961 tctcaacgct ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct
     4021 gatagtaaac gtcatagccc gccagctgga tcacgcgaat gccaacgtcc tgggcgaact
     4081 ggatggcttt acgcataatc tccagcccct gcgcccgcac cgcgtcatct tcgctgccca
     4141 acgggaaacg acgatgggca gaaaggcaca tggacggcac acgcacgccg gtttcaacaa
     4201 tagcattcac cagcgccaga cgctgttcgc ggctccagtc aaggcgcgac aggcgctcgt
     4261 tagtttcatc taccgacatc tcgacaaaat cgaagcccaa cgtttttgcc agtcgcaggc
     4321 gttccagcca gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt
     4381 tggacaacat atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgcg
     4441 cggcttcaac cggagaagcg gcatcacgga tgctgcgacc ggcgataaac acatgaattg
     4501 gaatgccttt gaacagcggc agatcttcca gcgccaggcc accggtgacg gtgactttga
     4561 agcccatatc ggaaagacgt ttgatcgcag tgatatcggc ttcaccccac gccacgccag
     4621 cggcctgcgc gtcacggctg cggtgataaa ccacctgctg aatacctgcg tcgcgccact
     4681 gttgcgcctg ttcccaggtc cagtaaccgg tcagttcgat ctgcacgtcg ccgttaaact
     4741 cttttgctac gtccagcgca cctttggcgg tgttgatatc cgcacaacaa atcacggtca
     4801 cccagtcagc gttggcttcg aagcacatac gagaaagaat tttgcccgca tcggcaattt
     4861 tggcgtctgc cagcacgatt ttgtgcgggt agagcgcttt cagatcgcga accgcacgca
     4921 cgccttcgcc cacgcacaga atggtaccca cttcgataat gtcgacttct tcggcaatca
     4981 ggcgagtggt ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg
     5041 gtaatgacat cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt
     5101 acttcctgct cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc ttcaaacagg
     5161 ttgacgatct gcatgatgcc cacttcctgg tgcgtgttgg catcgaccgc cgccatggtg
     5221 atgaggatat ccaccgggtc gttatcttcg tggttgaact ccagcggctt tttcagcgtc
     5281 accagcgcaa aaccggtttt cttaacgccc tcttccgggc gaccgtgcgg cattgccagg
     5341 cctggtgcga gcacaaagta cggaccgtgc tgttcaaccg catccagaat cgcctggtag
     5401 taacgcggct cgacaacatc cgccgccacc agcaggtcaa caccgatttt tactgcgtcc
     5461 tgccatgtct cagcttcagc ctgcaggcgg atggatttat tctccgccag cgaatcatgt
     5521 aatttcatgg cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc
     5581 agtttcgggc caaagtcggc aggagagagc atgttgcgca cgccaaccac gtatttgttg
     5641 ccggtcacgg tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc
     5701 aactcgcttt tgtattcgcc aaccgcgcag ctatttaccg tgtggtcaat gtttgattgg
     5761 gttaaaaact ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac
     5821 acagccagaa tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccaat
     5881 tgtttttctg catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt
     5941 gcaattacaa tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc
     6001 ggcggtgcca gaatcgacca gtccgccatc cccatccagg cactcatacc ggtaagtttc
     6061 accacccaga cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc
     6121 gcacgccagc cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc
     6181 gggataaagc ctgggataat caggatcgag gaaccgcagg cgaccaggat gccaaccgca
     6241 atcagctgac cgatggtgcc ccacataaag ccccagacca cggcgttcgg agcgaagcta
     6301 taaatagccg cacagtcaat cgccagaacc gcaccaggga tcaggcgctg ggagataccg
     6361 ttaaacgctt cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc
     6421 gccaccgcga aggaaaaacc ggtttgcagg atatagaccg tccagtgcac tttgcctgcc
     6481 atcgcctgca cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg
     6541 acaatcgccg tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg
     6601 tcttcaacgc tttcctcttt tttgccgagg aacggcgcaa ctttataggc aattaatgat
     6661 gcaaactgtt gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc
     6721 ttgtacatca tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata
     6781 atggttgtcc acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc
     6841 tggaacatga tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc
     6901 acgtaacaga tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca
     6961 tcgcccatgc ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta
     7021 atgccgtaga cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg
     7081 gaccctgcct gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg
     7141 ctgacacttt tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacagg
     7201 gcattggtca tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg
     7261 atctcctgaa gaagaggtaa ccgggtattc cgcacacccg gaatgttgtg cactcactct
     7321 aattttcaaa agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat
     7381 aattccacat ggatagagtg ggattcacac aacgaaacaa ttatttattt aaattcattt
     7441 agttaattta actacacatt ccattaaatc tttccccact caaaatcaac aacaacttcc
     7501 ctggcaaaaa gtcaaaaaat ggtcattacc ttcctgaagg aatagtgtca ggattaatca
     7561 aaaccaatca catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag
     7621 tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc
     7681 tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag
     7741 gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt
     7801 gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc
     7861 gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt gttcttgatc
     7921 cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg
     7981 acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca ttcattggac
     8041 cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg
     8101 tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg
     8161 accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg
     8221 gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca
     8281 gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg
     8341 tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg
     8401 atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca
     8461 tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag atcaaaaaag
     8521 atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc
     8581 cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta
     8641 ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg
     8701 gagaagttcg cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa
     8761 tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat
     8821 gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc
     8881 tgccactgcg cgacgcgata tcaataaact tggcgaaagc ggcaaactga aaaaggtgcg
     8941 caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca
     9001 ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatcc
     9061 gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg
     9121 tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca
     9181 agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt ccatcacttt
     9241 aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggaaa
     9301 agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg cagagcagaa
     9361 gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg
     9421 cgcgggaatg ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc
     9481 taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag
     9541 atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac
     9601 gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg
     9661 caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag
     9721 aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac
     9781 aattttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga
     9841 acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg
     9901 gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag
     9961 acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg
    10021 catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt cgccactaaa
    10081 acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc
    10141 aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa aaattacgca
    10201 cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag
    10261 ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa
    10321 tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct
    10381 ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa
    10441 agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg
    10501 gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc
    10561 acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg
    10621 gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc
    10681 agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga
    10741 cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag
    10801 acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat
    10861 aaattaccgg ctggaagctt gggcgtttat taccgcgatg gaatgccctg caacatgtgg
    10921 ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag
    10981 tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga
    11041 cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg
    11101 tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta
    11161 ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag
    11221 tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa
    11281 ttttcacata tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca
    11341 cacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta
    11401 cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat
    11461 atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca
    11521 gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc
    11581 gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct
    11641 tattgagaac gcgcaacaca tccaggcaca taatattgcc gg
//
LOCUS       DAZPDE010000119        11613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPDE010000119 DAZPDE010000000
VERSION     DAZPDE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.119"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            3..1196
                     /gene="gdhA"
                     /locus_tag="ACHU0J_002910"
     CDS             3..1196
                     /gene="gdhA"
                     /locus_tag="ACHU0J_002910"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1217511.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
     gene            complement(1313..2353)
                     /locus_tag="ACHU0J_002911"
     CDS             complement(1313..2353)
                     /locus_tag="ACHU0J_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1217512.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(2480..4441)
                     /gene="topB"
                     /locus_tag="ACHU0J_002912"
     CDS             complement(2480..4441)
                     /gene="topB"
                     /locus_tag="ACHU0J_002912"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1217513.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(4446..5489)
                     /gene="selD"
                     /locus_tag="ACHU0J_002913"
     CDS             complement(4446..5489)
                     /gene="selD"
                     /locus_tag="ACHU0J_002913"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1217514.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(5606..6157)
                     /locus_tag="ACHU0J_002914"
     CDS             complement(5606..6157)
                     /locus_tag="ACHU0J_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1217515.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6318..8174
                     /gene="sppA"
                     /locus_tag="ACHU0J_002915"
     CDS             6318..8174
                     /gene="sppA"
                     /locus_tag="ACHU0J_002915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1217516.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            8341..9357
                     /gene="ansA"
                     /locus_tag="ACHU0J_002916"
     CDS             8341..9357
                     /gene="ansA"
                     /locus_tag="ACHU0J_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1217517.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            9368..10009
                     /gene="pncA"
                     /locus_tag="ACHU0J_002917"
     CDS             9368..10009
                     /gene="pncA"
                     /locus_tag="ACHU0J_002917"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1217518.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(10102..11460)
                     /locus_tag="ACHU0J_002918"
     CDS             complement(10102..11460)
                     /locus_tag="ACHU0J_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217519.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
BASE COUNT         2883 a         3042 c         2859 g         2829 t
ORIGIN      
        1 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
       61 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
      121 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
      181 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
      241 gcagcgattt cgatccgaaa ggaaaaagcg aaggtaaagt gatgcgtttt tgccagacgc
      301 tgatgactga actgtatcgc cacctgggcg cggataccga cgttccggca ggtgatatcg
      361 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
      421 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
      481 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
      541 ttgaagggat gagcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
      601 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
      661 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
      721 atggtcgagc ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
      781 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
      841 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcatatatgc
      901 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
      961 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     1021 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     1081 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     1141 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     1201 taaatgcctg atggcgctac gcttatcagg cctacagatg ggcacaattc attgcagtta
     1261 cgctctaatg taggccggtc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     1321 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     1381 agtgctccca aaactgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     1441 ttctgcgata gcagcagata tgccagtaaa gaaatcccat tagactattt ttttgataat
     1501 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     1561 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag atcatccatg
     1621 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     1681 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     1741 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     1801 ccattaaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     1861 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     1921 tgccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     1981 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     2041 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     2101 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     2161 tcaaaccgtt ggatgttatc gctgcgaata caaacattac agcgacaaca catgttaaat
     2221 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcacccgt tcaaaattac
     2281 tattaaatat tccccagcca ttaaaagaat acttctcgct cccaggatgg ttttgcaata
     2341 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     2401 caaaaaaata tccaacgaac tttcttgatc cgctggatat ttcagaatat gactcgaata
     2461 gcacgaaaga ttcgctcgct tacgctatcg ccccgcttcc ggcttcatct gctggcggac
     2521 tttttttcgc actacgttta cgcggtgcag cctttttctt atcagcactg ccaccactgc
     2581 ccggagccac aatgccgcga aactgccgca ccggcgtacg tttggcttga tcaataagct
     2641 gatatagcgt ccccaccagc ggctgcataa agtcctgata gcgacactgc ttttcgctga
     2701 tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg tcgcgtcgcc atttctggca
     2761 gcgaatggaa taacgctttt ccggcgtcgg tggagtggat atagcgccct tttttggtca
     2821 gaaaaccacg cttgaacagc agttcaataa tcccggcacg ggttgcttct gtccccagac
     2881 catcggtcgc acgaaggacc tttttcagat ctttgtcctg cacaaagcgc gcgatcccgg
     2941 tcatcgccga aagcagtgtt gcatcggtaa aatggcgcgg cggctgggtt tgccgctcta
     3001 ccacttcacc tttttcacac agcaactcat cgcctttcgc caccacaggc aatggcgtgc
     3061 cgtcgttttc ttcatcgcgc tctttgctgc ctaacagcgt gcgccagcct gcttcagcaa
     3121 gaaaacgggc tttagcgaca aatttgcctt tggcaatgtc cagttcgata acacacttgc
     3181 ggaacaccgc atccgggcag aactgcatca ggtattgacg ggcaatcagg ttatagacct
     3241 tcgcttcgtt ctccgtcagg ttgatcgcag aactccgtgc ggtcgggatg atggcgtggt
     3301 gcgcatcgac ctttttgtca tcccaacagc ggttgcgtat atctggatct accactggct
     3361 gcggcaacag atccggtgca tgaacgctga tggcattcat caccgcgtgg cgtccggcaa
     3421 aatgttcttc tggcaaatag cgacaatcgg aacgcggata agtgattagc ttgtgcgttt
     3481 cgtacagctt ctggcagata tcaagcacgt tctgcgcgct cagaccgaag cgttttgccg
     3541 cttcaatctg caacgccgaa agcgaaaacg gcagcggcgc ggattctgat tcccgtttat
     3601 cgttatagct ggtgacaata gccggttgac cactaatgcg gttaaccaca tgctccgcca
     3661 gtggacgatg taacaagcgc ccttcttcat cctggtacgg ttcacacgct tcgctcggtt
     3721 gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat atgtgctttg acttcaaaga
     3781 agtctttcgc cacgaagttt tcaatctctt catcgcggcg caccaccagc ccgagcacgg
     3841 gtgtctgcac gcgtcccacg gaaagtacgc cctgataacc ggcattgcga ccgagaatgg
     3901 tatacgcacg ggtcatattg atgccgtaca gccagtcggc acgcgctcgc gccagcgcag
     3961 aaacgcacaa tggcacaaac tcactgttgg aacgaagacg gtcgatcgcc cgctcaaccg
     4021 cctgcgggtt caggtcgttt atcaagcaac gctgcacctg ctggcgcttt tccggtgcca
     4081 gttgcagata gtccagcact tcatccacca gcaattgccc ttcacgatcc gggtccccgg
     4141 cgtgaacgat ttcgctggct tcatgcagga agcgtttgat gacgttaagt tgtttggtca
     4201 cggagggtcg tggctgtaat tgccactttt ccgggacaat cggcaaatcc gcaagattcc
     4261 agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg ctcaagcagg tgaccgatac
     4321 accaagtcac cacctgacca ttaccgcact cgataaagcc atcgcctttc cggtgcggtt
     4381 tgggcaggac atcagcaatg gcgcgcgcca gactcggttt ttcggcaata aacaaccgca
     4441 ttaaattaac ggatctcaac catggcacga ccgccgcgcg ctggcaccag ttcgccaatc
     4501 gccgtcagtt caatgccaaa ctcggcggct gtagttttaa cctcattttc tgcttccggc
     4561 atgaccgcca gcagcaaacc gccagaagtt tgcggatcgc acagcagatc gcgcacttca
     4621 cgcggcattt cacccatcag atgaccgtag ctggcaaagt tacgttcagt gccgccaggt
     4681 actgcgccca acttaatgta ctcttcaaca ccggggagtt tcgggatcgc ttcatagtcg
     4741 acgcgtgcct gcacaccagc cccctgacac atttcgctca agtggcccag cagaccaaag
     4801 cccgtaacgt cggtcatcgc ttttacgcct tcgatgttgg caaaggacgc gcctgcgatg
     4861 ttcatccggc acatcacttc cgtcgccagt ccctgatgtt ctggtttcaa cagtgatttt
     4921 ttctcagccg tggtaagaac gccgatcccc agcggtttcg tcaggaacag tttgcatccg
     4981 gcttgtgcgg tactgttttt cttcacccgc tcggtcggta cgatccccgt taccgccaga
     5041 ccaaaaatcg gctccggcgc atcgatggag tgaccgccag ccagcgcaat acccgcctga
     5101 cggcatgcat agcgtccacc ttcggttact tcgcgggcaa tttccgggga aagtttgtta
     5161 atcggccagc cgaggatcgc aatcgccata atcggtttgc cccccatcgc gaagatatcg
     5221 ctgatggcgt tagtcgccgc aatgcggcca aaatcgaaag gattatcaac gatcggcata
     5281 aagaagtcgg tggtactgat aacgctggtg ccattgccca gatcgtacac cgccgcatcg
     5341 tcgcgggttt cattacccac aagcaaattc ggatcaacaa acttcgcctg ctcactgtgc
     5401 aggatggttt ccaacacttt tggggaaatt ttacagccgc aaccagctcc gtggctgtat
     5461 tgggtcaaac gaatcgagtt ctcgctcatg gacatctcct gtcaatgcaa tccgggtatg
     5521 gtaaccctca ttccgtgaag tgataagtga gagtgtctga attcctgcgc ctttgctcac
     5581 aatccagaca gtttcgcgac aattatcaga aataagttac aaacggcgtc gggtccggga
     5641 cgttaatcga cgtagatgct ttcaactgcg gcgtgccgag gtagagaaaa ccgacaattt
     5701 tatcctgctc acggcaaccg aatgcttcac gcactaccgg actttcagtt aatgcgccac
     5761 tgcgccagat gccgccaaat ccctgggcaa cggctgccat ttgcatcgcc atgaccgcgc
     5821 atccggcaga catttcctgt tcccatcgcg ggactttatg attttctttg catttcgcca
     5881 ccaccgtgat gatgagcggt gcgcggaacg gcgcattacg ggctttgtcg atagctttat
     5941 catcactacc ggcagcaatc gccccctgtt ccagtacggc gctgaaacgc tcgcaccctt
     6001 ccccttcaat cacaaaaaaa tgccacggtt gcatggactt atggtccggc gcacgcatac
     6061 ccgcacgcag gatgttttgc agttgttcac ccgttggcgc gggttcagcc aggcgggagg
     6121 cgctacggcg attgatcaat agttcgagtg catccatttg attaactcct gtcgtgatat
     6181 ttattcacaa aattaacacg agagtggatt ttgttacagc acagtccgca attcctgctg
     6241 acaagtgccg gttgggtcat tacgataacc acatctattg cgcctgtgac aggtgtgacc
     6301 ttaagttggg agaatacatg cgaacccttt ggcgatttat tgccggattt tttaaatgga
     6361 cgtggcgtct gctgaatttc gtccgtgaaa tggtacttaa cctgttcttt attttcctcg
     6421 tactggttgg tgtggggatc tggatgcagg tcagtggtgg tgattcgaaa gaaacggcca
     6481 gtcgtggcgc actgctgctg gacatttctg gtgtgatcgt cgataaacca gacagttctc
     6541 agcggtttag taaattaagc cgccagctgc ttggtgccag ttccgatcgt ctgcaggaaa
     6601 actcactgtt tgatatcgtc aacaccattc gccaggcgaa ggacgaccgc aatatcaccg
     6661 gtatcgtgat ggatctgaaa aacttcgcag gcggcgacca gccgtctatg cagtacatcg
     6721 gcaaagccct gaaagagttt cgtgacagcg ggaaaccggt ttatgccgtt ggcgagaact
     6781 acagccaggg gcaatattat ctcgccagtt tcgccaataa aatttggctg tcaccgcaag
     6841 gcgtggttga tctgcacggt tttgctacca acggtctgta ctacaaatcg ttgctggata
     6901 agctgaaagt ttccacccat gtgttccgcg tgggtacgta taaatctgcc gttgaaccgt
     6961 ttattcgtga tgatatgtca ccggcagccc gcgaagctga cagccgctgg attggtgagc
     7021 tgtggcaaaa ctatctgaat actgttgccg ctaaccggca gatccctgct cagcaggtat
     7081 tccctggcgc gcaagggttg cttgagggtt taaccaaaac cggtggcgat accgcgaaat
     7141 atgcactgga aaacaagctg gtcgatgcac tggcatcgag tgccgaaatc gaaaaagctc
     7201 tgaccaaaga gttcggctgg agtaagactg ataaaaatta tcgcgccatc agttattacg
     7261 attacgcatt gaaaacgccg gcagataccg gtgacagcat cggtgtcgtc ttcgctaatg
     7321 gcgcaattat ggatggcgag gaaactcagg ggaatgttgg cggtgatacc actgcggcac
     7381 aaatccgcga cgctcgcctt gacccgaaag tgaaagcgat tgtcctgcgt gttaatagcc
     7441 caggcggcag cgttaccgcg tctgaagtga ttcgcgctga actggcagca gcccgggcag
     7501 cgggtaagtc tgtggttgta tcgatgggcg gcatggcggc atctggtggt tactggattt
     7561 ccacgccagc taattacatt gtggctaacc ccagcaccct gaccggttct atcggtatct
     7621 tcggcgtgat caccaccgta gaaaatagtc tggattcgat tggtgttcat actgatggtg
     7681 tctcaacttc accgctggcg gatgtttcta tcaccagggc actgccgccg gaagcgcagc
     7741 agatgatgca attaagcatt gagaatggct ataaacgctt tatcacgctg gttgctgatg
     7801 cgcgtcattc gacgccggag caaattgata aaatcgccca gggccacgtc tggaccggtc
     7861 aggatgcaaa agctaacggg ctggtcgata gtctcgggga tttcgatgat gcggttgcca
     7921 aagcagcaga gctcgcaaaa gtgaaacagt ggcatctgga atactacgtt gatgaaccga
     7981 ccttcttcga caaagtgatg gacaacatgt ctggttctgt ccgggcaatg ttgccagatg
     8041 cgttccaggc catgttacct gcaccgcttg cctcggtagc ctctaccgtt aaaagtgaaa
     8101 gcgacaagct ggccgcgttt aacgacccac aaaaccgtta tgcgttttgc ctgacctgcg
     8161 ccaacgtgcg ttaagtcttg tactgagtgg ccgacagatc gtcggccaca ttatttttta
     8221 cctcgacgaa tcctcttccc gctgtctcgc cccatatttc cttatccgca tagtatcagg
     8281 tgcgctcctc ctgcctcacg tatatacttt tgctctttcg atatcattca tatcaatatc
     8341 atgcaaaaga aatcaattta cgtcgcctac acgggcggga ccatcgggat gcagcgttcc
     8401 gagcagggtt atattccggt gtcaggtcat ctacaacgcc aactggcgct gatgccggaa
     8461 ttccatcgcc cggagatgcc agatttcacc attcatgaat atacgccgtt gatggattct
     8521 tctgatatga cgccggaaga ctggcagcat attgctgaag atattaaagc gcactatgac
     8581 gactatgatg gttttgtcat tctacacggt accgacacga tggcgtatac cgcctctgcg
     8641 ctgtcgttca tgctcgagaa tctcggtaaa ccggtcattg tgacagggtc acaaatcccg
     8701 ctggctgagt tacgctctga cggacaaatt aatctgctga atgcgttgta cgttgcgccg
     8761 aattatccga tcaacgaagt aacgctcttt ttcaataacc gattgtatcg cggcaaccgc
     8821 actaccaaag cccatgccga tggttttgat gcgtttgcct ctccaaacct tcctccgtta
     8881 ctggaagctg gtatccatat tcgtcgtttg aatacgccac ccgccccgca cggtgaaggg
     8941 gaattaatcg ttcatccaat cacgccacaa ccaattggcg tggtgacgat ttatccaggg
     9001 atttctgctg acgtcgtgcg caattttctg cgccaaccgg tgaaagcatt gattctgcgc
     9061 tcctatggcg tgggtaatgc gccacaaaac aaagccttcc tgcaggaatt acaagaagcc
     9121 agcgatcgcg gtattgtggt ggtcaacctg acacaatgta tgtccggtaa agtgaatatg
     9181 agtggttatg ccaccggtaa cgccctcgcc catgccggcg ttattgacgg tgcagatatg
     9241 actgtagaag ccacgctaac caaactgcat tacctgctga gccaggaact ggatactgaa
     9301 accattcgca aggccatgag ccaaaacctg cgcggcgaac tgacgccgga tgattaagga
     9361 gactgcaatg ccccatcgcg ccctgttact ggtcgattta caaaatgatt tctgtgctgg
     9421 tggcgcgctc gccgtaccgg aaggtgacag tacggtggag gttgctaacc gccttattga
     9481 ctggtgccag tcgcgcggag aagcggttat cgccagccag gactggcatc cggcgaatca
     9541 cggcagcttt gccagccagc acggcgtaga gccttatacg ccaggccaac tcggcggcct
     9601 gcggcaaact ttctggcctg aacactgtgt gcagaacagt gaaggcgcac aattacatcc
     9661 gttactgaat caaaaagaga tcgcagcggt gttccataaa ggcgaaaatc ctttagttga
     9721 cagttacagt gccttttttg ataacggacg tcggcagaaa accgcgcttg atgactggtt
     9781 gcgcgatcat gaaatcgatg aattgatcgt tatgggcctg gctactgact attgcgtgaa
     9841 gtttaccgtg ctggacgcgt tacagttagg ttataaggta aacgtgatta ccgatggctg
     9901 tcgtggcgtg aatatccagc cccaggacag tgcacacgcg tttatggaga tgtcagcagc
     9961 tggggcaacg ctatatacgc tggcagactg ggaagagacg caggggtaat tttacgctgg
    10021 cctacaattc tgtactggca ttgtaggcca aataaaacac gtcagtggta catctggcaa
    10081 ttgatgccat caacgaaaga tttaattcac ctcagaaatc tcttccagcg acaccttccg
    10141 cgtttcgatg ccaaaaatgg agagaaccag cgcacagagc actaacatga caccaagcac
    10201 cataaacacc gtgatcgacc cataatgtgt taataatgcc gcaacgccat agggcgtgaa
    10261 aactgcgacg atccgtccga cggcattaac gaaacccgaa ccgcgcaggc gtaaatgcgt
    10321 tggccaaagc tccgggatat aaaccgccga cgcgaagcaa acatacatgt ataaaaagaa
    10381 gatcatcacc agaccataga ttaaaatcgc ccactctgta gtctgacttg aatagatata
    10441 gcctaacacg gcaataataa taagtaaggc ggagccaaat aatcgacgag gaaaatgatc
    10501 aataattaat gccgcaataa atattcctac cggagcacca atcataataa cagcggtcat
    10561 taatattgat ttatcgacat caatgccgga gttaacaaat atggtcggta tccatacggt
    10621 aatggtataa agcgaaatgt tcattgcaat taaaacagtt atcgcaacta atgtacgtcg
    10681 taacatttcc cctttaaaca ggagcctgaa ggtacccttg attaccttaa ccttgctgtt
    10741 gctctggttc aaagtaagct ggggtaaacg aatactcttc tccctttcaa tttgctgctc
    10801 tacttcacga agttggcttt ctgcaccggc gatttgccct ttcccttcca gccatcgtgg
    10861 cgactcgata aagtatttac ctgagagaaa ccaggctaac agtatgccaa taccacccag
    10921 aagaaacatt attcgccaac taaaaaaagc gataaccacc acgcctatcg ccgcagacag
    10981 catgggcgac cagttaccaa caaatgagag ccgcgcggac cattttccac gcaccgtcgc
    11041 ggggataaac tccgtaaatg aggcatagcc aaccataatc agcgccccca ttcctgttcc
    11101 catcaggaag cgaaagaaaa tgagccagta catatcaggg acaaaagcgg cccctgttgc
    11161 agcaataccg acgatgagaa gatttatgcg aaacgccctg cgccgcccaa agtagtcacc
    11221 aataaaccca ccagtaagtg agccgatgaa ataaccaaac attaatgccg aggtaaaggc
    11281 ggcatagagg aaattatttg accatccatt gcttaccagc tttgctaaga cgacattacc
    11341 tgagtaactc aaaaaccccg ttaataacag actaaagctt attataccaa atatacgata
    11401 atgaaaccgg gccaaaggca agcggtccag tcttgcgcca atttgatcat attgttccat
    11461 tgggtgcctc ggatttgcta taaaaggtag caattgcggc aaattcactt gccgcatctt
    11521 tatcatcatt ttatttccag tcgcaggaac aaataatatt gacaacgtgt gcttaaatta
    11581 ccacaccagc tggatattat tgtctttgca aaa
//
LOCUS       DAZPDE010000120        11417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPDE010000120 DAZPDE010000000
VERSION     DAZPDE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.120"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHU0J_002919"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHU0J_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHU0J_002920"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHU0J_002920"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1217520.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHU0J_002921"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHU0J_002921"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR1217521.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHU0J_002922"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHU0J_002922"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR1217522.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHU0J_002923"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHU0J_002923"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR1217523.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHU0J_002924"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHU0J_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR1217524.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHU0J_002925"
     CDS             complement(8300..11167)
                     /locus_tag="ACHU0J_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1217525.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2747 a         2813 c         2847 g         3010 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttact
//
LOCUS       DAZPDE010000121        11345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPDE010000121 DAZPDE010000000
VERSION     DAZPDE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.121"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            240..539
                     /locus_tag="ACHU0J_002926"
     CDS             240..539
                     /locus_tag="ACHU0J_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1217526.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(659..838)
                     /locus_tag="ACHU0J_002927"
     CDS             complement(659..838)
                     /locus_tag="ACHU0J_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1217527.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            912..2273
                     /gene="pabB"
                     /locus_tag="ACHU0J_002928"
     CDS             912..2273
                     /gene="pabB"
                     /locus_tag="ACHU0J_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1217528.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2277..2855
                     /locus_tag="ACHU0J_002929"
     CDS             2277..2855
                     /locus_tag="ACHU0J_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1217529.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            3039..4403
                     /gene="sdaA"
                     /locus_tag="ACHU0J_002930"
     CDS             3039..4403
                     /gene="sdaA"
                     /locus_tag="ACHU0J_002930"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1217530.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4534..6132
                     /locus_tag="ACHU0J_002931"
     CDS             4534..6132
                     /locus_tag="ACHU0J_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1217531.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6136..7686)
                     /gene="yoaE"
                     /locus_tag="ACHU0J_002932"
     CDS             complement(6136..7686)
                     /gene="yoaE"
                     /locus_tag="ACHU0J_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1217532.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7674..7832)
                     /gene="yoaL"
                     /locus_tag="ACHU0J_002933"
     CDS             complement(7674..7832)
                     /gene="yoaL"
                     /locus_tag="ACHU0J_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1217533.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8149..9120
                     /gene="manX"
                     /locus_tag="ACHU0J_002934"
     CDS             8149..9120
                     /gene="manX"
                     /locus_tag="ACHU0J_002934"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1217534.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9183..9983
                     /gene="manY"
                     /locus_tag="ACHU0J_002935"
     CDS             9183..9983
                     /gene="manY"
                     /locus_tag="ACHU0J_002935"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1217535.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9996..10847
                     /gene="manZ"
                     /locus_tag="ACHU0J_002936"
     CDS             9996..10847
                     /gene="manZ"
                     /locus_tag="ACHU0J_002936"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1217536.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10902..>11345
                     /locus_tag="ACHU0J_002937"
     CDS             10902..>11345
                     /locus_tag="ACHU0J_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1217537.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2785 a         2866 c         2852 g         2842 t
ORIGIN      
        1 cgccctttga ttagcaactc tggttttctt tcttagctga acaatctggt tatagcgtgc
       61 gggctgaccg ttaattttct ccatcttact ggataacaca ccatgagctg ctacctttaa
      121 aacacctgaa tgtcaacagg ttaactcgtg ccatatcgtt ataatccctt tgatattaga
      181 tgcaaattaa ggtcatatat agccttatcg ataacatggt taattttaag gataagaata
      241 tgcctgctgt aatagataaa gccctggatt tcattggtgc catggatgta tcagcgccaa
      301 caccaagttc gatgaatgaa agcacggcga agggaatctt taaatattta aaagaactgg
      361 gagtacccgc gagtgccgct gatattacgg cgcgagctga ccaggaaggc tggaaccctg
      421 ggttcacgga aaaaatggtt ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga
      481 ttaaaaatcc tgaatacttt tcaacatata tgcaggaaga actaaaagca ctggtctgag
      541 ttaaatttat atcagcataa atgggtcagg acgcttttaa tcacatataa aaagcgtcct
      601 ttttctcaca atcaaatgat catcggcttc tataacgaaa tcgatacgga ttagtgtgtt
      661 actcgtcttc atcctcaaaa cgcgccacga ttagctcgcc ggagtggttg gcacgcagct
      721 cttctgccac cagcgcgatt gcctggccgc tactcatccc ttgtgccatc agttcctgga
      781 tccgctcgac agctttttgc tgctgttcat ggatgagtga aggtaaacct gcaaacattg
      841 ttaactcctg ctaaattgtt ggcgctaatt atttcatgct acccggcaca tagccagtag
      901 agtcaggact gatgaagacg ttatctccca ctgtgattac tttaccctgg cgtcaggacg
      961 ccgctgaatt ttatttctcc cgcttaagcc acctgccgtg ggcgatgctt ttacactccg
     1021 gctatgccga tcatccgtat agccgctttg atattgtggt cgccgatccg atttgcactt
     1081 taaccacttt aggtaaagaa accgttgtta gtgaaagcga aaaacgcaca acgaccactg
     1141 atgacccgct acaggtgctc cagcaggtgc tggatcgcgc agacattcgc ccaacgcata
     1201 acgaagattt gccatttcag ggcggcgcac tggggttgtt tggctacgat ctgggccgcc
     1261 gttttgagtc actgccagaa attgcggaac aagatatcgt tctgccggat atggcagtgg
     1321 gtatctacga ttgggcgctc attgtcgacc accagcgtca tacagtttct ttgctgagtc
     1381 ataatgatgt caatgcccgt cgggcctggc tggaaagcca gcaattctcg ccgcaggaag
     1441 atttcacgct cacttccgac tggcaatcca atatgaccca cgagcagtac ggcgaaaaat
     1501 ttcgccaggt acaggaatat ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc
     1561 gttttcatgc gacctattct ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca
     1621 accgcgcgcc atttagcgct tttttacgtc ttgaacaggg tgcaatttta agcctttcgc
     1681 cagagcggtt tattctttgt gataatagtg aaatccagac tcgcccgatt aaaggcacgc
     1741 taccacgcct gcccgatcct caggaagata gcaaacaagc agaaaaactg gcgaactcag
     1801 cgaaagatcg tgccgaaaat ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg
     1861 ttgccgtagc cggttcggta aaagtaccag agctcttcgt ggtggaaccc ttccctgccg
     1921 tgcatcatct ggtcagcact ataacggcgc gactaccaga acagttacac gccagcgatc
     1981 tgctgcgcgc agcttttcct ggtggctcaa taaccggggc tccgaaagta cgggctatgg
     2041 aaattatcga cgaactggaa ccgcagcgac gtaatgcctg gtgcggcagc attggctatt
     2101 tgagcttttg cggcaacatg gataccagca ttactatccg cacgctgact gccattaacg
     2161 gacaaatata ctgctctgcg ggcggtggaa ttgtcgccga tagccaggaa gaagcggaat
     2221 atcaggaaac ttttgataaa gttaataaga tattacgcca actggagaag taagacgtgg
     2281 aataccgtag cctgacgctt gatgattttt tatctcgctt tcaacttttg cgcccgcaaa
     2341 ttaaccggga acccctaaat catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac
     2401 cgcaaccggg gttgttgctg actcagcgtt caattcatct gcgtaaacac gctggacaag
     2461 tggcattccc tggaggtgca gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc
     2521 gcgaagctga agaagaggtc gctataccgc cttccgccgt tgaagttatc ggcgtgctgc
     2581 cgcccgtcga tagcgtaact ggctatcaag taaccccagt ggtcggcatt atcccgcccg
     2641 atctgccgta tcgcgccagt gaagatgaag tctcggcggt gtttgaaatg ccgctcgccc
     2701 aggcattaca tctgggtcgt tatcaccctt tagatatcta ccgccgtggc gattcacatc
     2761 gggtatggct gtcctggtac gaacagtatt ttgtatgggg aatgaccgca ggcataattc
     2821 gtgagctggc gctgcaaatt ggtgtgaccc cctgactata cttatcttta catctacaaa
     2881 acactacttg agacaatcat cgcaatatta gttaaatcgc ggtttttgat tagtttaatt
     2941 catgtgaata gttaagccag tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc
     3001 gttactggaa gtccagtcac cttgtcagga gtattatcgt gattagtcta ttcgacatgt
     3061 ttaaggtggg gattggtccc tcatcttccc ataccgtagg gcctatgaag gcgggtaaac
     3121 agttcgtcga tgatctggtc gaaaaaggct tactggatag cgttactcgc gttgccgtgg
     3181 acgtttatgg ttcactgtcg ctgacgggta aaggccacca caccgatatc gccattatta
     3241 tgggtcttgc aggtaacgaa cctgccaccg tggatatcga cagtattccc ggttttattc
     3301 gcgacgtaaa agagcgcgaa cgtctgctgc tggcacaggg acggcatgaa gtggatttcc
     3361 cgcgcgacaa cgggatgcgt tttcataacg gcaacctgcc gctgcatgaa aacggtatgc
     3421 aaatccacgc ctataacggc gatgaagtcg tctacagcaa aacttattat tccatcggcg
     3481 gcggttttat cgtcgatgaa gaacactttg gtcaggatgc tgccaacgaa gtgagcgtgc
     3541 cgtatccgtt caaatctgcc accgaactgc tcgcgtactg taatgaaacc ggctattcgc
     3601 tgtctggtct cgctatgcag aacgaactgg cgctgcacag caagaaagag atcgacgagt
     3661 atttcgcgca tgtctggcaa accatgcagg catgtatcga tcgcgggatg aacaccgaag
     3721 gtgtactgcc aggcccgctg cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgttag
     3781 tttccagcga taaactgtct aacgatccga tgaatgtcat tgactgggta aacatgtttg
     3841 cgctggcagt taacgaagaa aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg
     3901 gtgcctgcgg tatcgttccg gcagtgctgg cttactatga ccactttatt gaatcggtca
     3961 gcccagacat ctatacccgt tactttatgg cagcgggcgc gattggtgca ttgtataaaa
     4021 tgaacgcctc tatttccggt gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt
     4081 caatggctgc tgcgggtctt gcagaactgc tgggcggtag cccggaacag gtttgcgtgg
     4141 cggcggaaat tggcatggaa cacaaccttg gtttaacctg cgacccggtt gcagggcagg
     4201 ttcaggtgcc gtgcattgag cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac
     4261 ggatggctct gcgccgcacc agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga
     4321 tgtacgaaac cggtaaggac atgaacgcca aataccgcga aacctcacgc ggtggtctgg
     4381 caatcaaagt ccagtgtgac taatacttct tactcgccca tctgcaacgg atgggcgaat
     4441 ttatacccgc tttctcgtct gctgtaatat tccccactac acttccactg ttgcgtcagg
     4501 cgtttgtcgc catacgctta cagggtggcc cgcatgcaaa aagcacaacg gatcattaaa
     4561 acctatcgcc gtaatcgaat gattgtttgt acgatttgcg cactcgttac gctcgcttcg
     4621 accctgagcg tgcgatttat ttcacagcgt aacttaaatc aacaacgggt agtacaattc
     4681 gccaatcacg ctgtagagga attagataaa gtactgcttc ccctacaggc aggtagcaaa
     4741 gtcttgcttc cgctgattgg tctgccctgc tctgtcgccc atttgccatt acgtaaacag
     4801 gcggcaaaac tccaaactgt gcgatccatt ggcctggtgc aagacggcac actttattgc
     4861 tccagcattt ttggttatcg caatgtgccc gtcgtggaca ttctggctga acttcctgca
     4921 ccgcaaccac ttttacgcct gacgatcgac cgtgccctga ttaaaggcag tccggttttg
     4981 attcaatgga cgcctgcagc gggcagtagc aatgctgggg tcatagagat gattaacatc
     5041 gacttactga cggcaatgct ccttgagcca caactgccgc aaatcagtag cgccagcctg
     5101 acggtggaca aacggcattt gctctatggt aatgggctgg tagattccct tccgcaacct
     5161 gaagacaatg aaaactatca ggtttcttcg caaagctttc cttttaccat taacgtaaat
     5221 ggtccggggg ctacggcgct ggcatggcac tatcttccaa cacaattacc gctggcggtg
     5281 ctgctaagtt tactggtggg ctatatcgcc tggctggcga ccgcttaccg gatgagcttt
     5341 tcccgcgaaa ttaatctggg cctggcgcaa catgagttcg aattgttctg tcagcctttg
     5401 cttaatgcgc gcagccagca atgtattggt gtagagattt tgctgcgctg gaataatccg
     5461 cgtcagggct ggatttcacc ggatgtgttt attcctatcg cggaagaaca tcatttaatt
     5521 gtgccactga cccactatgt gatggcagaa accattcgtc agcaccatgt tttcccgatg
     5581 agtagtcagt ttcatgttgg cattaacgtc gcacccagcc attttcgccg tggtgtgctg
     5641 ataaaagatc tcaatcagta ctggtttagc gctcacccga ttcagcaact gatcctcgaa
     5701 atcaccgaac gcgatgcctt actggatgtt gattatcgga ttgcccgcga gctgcatcgt
     5761 aaaaacgtca aactggcgat tgatgacttc ggcaccggca acagctcgtt ttcctggctt
     5821 gaaacattac gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc tataggttct
     5881 gacgcggtta actcgacggt gaccgatatc atcatcgcgc tggggcaaag actgaatatt
     5941 gaactggtgg cggagggcgt ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc
     6001 gtgcatattt tgcaagggta tttgtacgca cagccgatgc cgctacgtga ttttcccaaa
     6061 tggctggcgg gcagccaacc gccgcccgcc cggcataatg gacatatcac gcccattatg
     6121 tcgttacgtt aacgcttact catcttcatc gtgcgccggt tgctctttaa caatgcgaac
     6181 cagatcaaca cgataatcat tggcttcaaa gatggtgata tgcagtggcc ctacatcaat
     6241 cacatcgccc acacggggaa tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc
     6301 gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc
     6361 tgtaccgcct tttaccagcc agccgttacc atcggtaatg atttccggcg tttcgtcagc
     6421 gtccgggaat tcacccgcaa tggcttccag cacatccagc ggcgtgacca agccttgtac
     6481 cacaccaaac tcgttggtca cgataacaaa gctcccgcga gcgcgacgca gcacgcccag
     6541 caggttgatc ggatcgaggg tttccgggac gataatcgcc ggagacgccg aagcaatcgc
     6601 cgccacatca acgccctctt ccagcgccac cagcagttct ttagcacgta cgataccgat
     6661 gatttcatcc agttcaccgc gacataccgg gaacagactg tgcggtgaag agagcagttg
     6721 ctcgcggatt tcatcgaccc cgagattagc gtcaacccag cttatttcac cgcgcggcgt
     6781 catgatcccg cgcagagaac gcgacgccag cgtcagtacg ccgttaatca tgtaccgttc
     6841 ttcttcggca aatgcacctt ccgggatcgg catcgggtta tcggcatcgt gctgaacatt
     6901 ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg gcagtacgcg ctcgcagcgg
     6961 caaagtcgac tggtgacgaa taaagttgcg acgcgcaatc tggttaaaca cttcgataat
     7021 gatcgagaag ccaatcgcgg catacaggta acctttcgga atgtggaaac cgaaaccttc
     7081 tgccaccaga ctcagaccaa tcattaacag gaagctcaga cagagcacca ccaccgtggg
     7141 gtgctggtta acgaatcgcg tcagcggttt cgatgccagc aacataaccg ccatcgcaat
     7201 cactaccgcc gccatcatca ccggcagatg gttaaccatc cctactgcag taattaccgc
     7261 atccaacgag aagacggcgt caaggatgac aatctgtgtg acgaccaccc agaaactggc
     7321 gtagccttta ccgtggccgg aatcatgatc gcggttttcc agccgttcat gcagttcggt
     7381 tgttgctttg aacagcaaga atatcccccc gaacaacata atcagatcgc gtccggagaa
     7441 ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg accatccatg aaatcagcga
     7501 cagcagcccc agacgcataa tcagcgccag tgataacccc agcaaacgcg ctttatcgcg
     7561 ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag accaggttat cgatacccag
     7621 cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa attgaggggt ccattaagaa
     7681 ttccatgaca agctcctgct taaggaatag ctattcgacg ccagaaataa tgcaggcgta
     7741 acgacaaaat gcaaacgaaa ggtgcggcat agagtgccag aaaggcaggc gttaaaaggc
     7801 ctgatgctga aatgacgtcg gtgacgatcc atactgcggg ctactgccct atactccatg
     7861 gttgttaaac gggagttaaa catatcagag acgcctctga tttggcaaag atttaccttc
     7921 ctttgcaaac gaatgtgaca aggatatttt acctttcgaa atttctgata atcgaaagtt
     7981 aaattacgga tcttcatcac ataaaataat tttttcgata tctaaaataa atcgcgaaac
     8041 gcaggggttt ttggttgtag cccttatctg aatcgattcg attgtggacg acgattcaaa
     8101 aatacatctg gcacgttgag gtgttaacga taataaagga ggtagcaagt gaccattgct
     8161 attgttatag gcacacatgg ttgggctgca gagcagttgc ttaaaacggc agaaatgctg
     8221 ttaggcgagc aggaaaacgt cggctggatc gatttcgttc caggtgaaaa tgccgaaacg
     8281 ctgattgaaa aatacaacgc tcagttggca aaactcgaca ccactaaagg cgtgctgttt
     8341 ctcgttgata catggggagg cagcccgttc aatgctgcca gccgcattgt cgtcgacaaa
     8401 gagcattatg aagtcattgc aggcgttaac attccaatgc tcgtggaaac gttaatggcc
     8461 cgtgatgatg acccgagctt tgatgaactg gttgcgctgg cagtagaaac aggccgtgaa
     8521 ggcgtgaaag cactgaaagc caaaccggtt gaaaaagccg cgccagcacc cgctgccgca
     8581 gcaccaaaag cggctccaac tccggcaaaa ccaatggggc caaacgacta catggttatt
     8641 ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg tcgccacccg ctggaccaaa
     8701 gaaaccaatg tctcccgtat tattgttgtt agtgatgaag tggctgcgga taccgttcgt
     8761 aagacactgc tcacccaggt tgcacctccg ggcgtaacag cacacgtagt tgatgttgcc
     8821 aaaatgattc gcgtctacaa caacccgaaa tatgctggcg aacgcgtaat gctgttattt
     8881 accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg tgaaaatcac ctctgttaac
     8941 gtcggtggta tggcattccg tcagggtaaa acccaggtga ataacgcggt ttcggttgat
     9001 gaaaaagata tcgaggcgtt caaaaaactg aatgcacgcg gtattgagct ggaagtccgt
     9061 aaggtttcca ccgatccgaa actgaaaatg atggatctga tcagcaaaat cgataagtaa
     9121 cgtattgtgt tgattatcat tcagttttca cacttaagtc ttacgtaaac aggagaagta
     9181 caatggagat taccactctt caaattgtgc tggtatttat cgtagcctgt atcgcaggta
     9241 tgggatcaat cctcgatgaa tttcagtttc accgtccgct aatcgcgtgt accctggtgg
     9301 gtatcgttct tggggatatg aaaaccggta ttattatcgg tggtacgctg gaaatgatcg
     9361 cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga cgccgctctg gcttctatca
     9421 tttctaccat tctggttatc gcaggtcatc agagcattgg tgcaggtatc gcactggcaa
     9481 tccctctggc cgctgcgggc caggtactga ccatcatcgt tcgtactatt accgttgctt
     9541 tccagcacgc tgcggataag gctgctgata acggcaacct gacagcgatt tcctggatcc
     9601 acgtttcttc tctgttcctg caagcaatgc gtgtggctat tccggccgtc atcgttgcgc
     9661 tgtctgttgg taccagcgaa gtacagaaca tgctgaatgc gattccggaa gtggtgacca
     9721 atggtctgaa tatcgccggg ggcatgatcg tggtggttgg ttatgcgatg gttatcaaca
     9781 tgatgcgtgc tggctacctg atgccgttct tctacctcgg cttcgtaacc gcagcattca
     9841 ccaactttaa cctggttgct ctgggtgtga ttggtactgt tatggcagtg ctctacatcc
     9901 aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc tcaggcagct ggtaacaacg
     9961 atctcgataa cgaactggac taacaggtga gcgaaatggt tgatacaact caaactacca
    10021 ccgagaaaaa actcactcaa agtgatattc gtggcgtatt cctgcgttct aacctcttcc
    10081 agggttcatg gaacttcgaa cgtatgcagg cactgggttt ctgcttctct atggtaccgg
    10141 caattcgtcg cctctaccct gagaacaacg aagctcgtaa acaagctatt cgccgtcacc
    10201 tggagttctt taacacccag ccgttcgtgg ctgcaccgat tctcggcgta accctggcgc
    10261 tggaagaaca gcgtgctaat ggcgcagaga tcgatgacgg tgctatcaac ggtatcaaag
    10321 tcggtttgat ggggccgctg gctggtgtag gcgacccgat cttctgggga accgtacgtc
    10381 cggtatttgc agcactgggt gccggtatcg cgatgagcgg cagcctgtta ggtccgctgc
    10441 tgttcttcat cctgtttaac ctggtgcgtc tggcaacccg ttactacggc gtagcgtatg
    10501 gttactccaa aggtatcgat atcgttaaag atatgggtgg tggcttcctg caaaaactga
    10561 cggaaggggc gtctatcctc ggcctgtttg tcatgggggc attggttaac aagtggacac
    10621 atgtcaacat cccgctggtg gtttcgacca tcaccgggca ggatggacag acccgtgtca
    10681 ccaccgtgca aactattctt gaccagttaa tgccaggcct tgtaccactg ctgctgacat
    10741 ttgcttgtat gtggctgctg cgcaaaaaag ttaacccgct gtggatcatc gttggcttct
    10801 tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg actgtaagac tgtcgtacac
    10861 taccggggcc ttttggcccc gtttttttat ctggaggatt aatgacaatc acggacctgg
    10921 tactgattct tttcatcgcc gcactcctgg ccttcgcgat ctacgatcag ttcatcatgc
    10981 cccgccgtaa cggccccacc ctgctggcaa ttcctttgct ccggcgtggt cgcatcgata
    11041 gcgttatctt cgtcggattg attgtcattc ttatctataa caacgtcacg aatcatggcg
    11101 cgctaataac aacatggtta ttaagtgcac tggctctgat gggtttttat atattctgga
    11161 tccgcattcc gaagatcatc tttaaacaaa aaggtttttt cttcgccaat gtctggattg
    11221 aatatagccg aatcaaagcg atgaacttgt cggaagatgg cgtgttggtg atgcaattag
    11281 aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga tctggaaaaa atttataagc
    11341 ttctc
//
LOCUS       DAZPDE010000122        11214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPDE010000122 DAZPDE010000000
VERSION     DAZPDE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.122"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHU0J_002938"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHU0J_002938"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1217538.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHU0J_002939"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHU0J_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1217539.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="ACHU0J_002940"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="ACHU0J_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1217540.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="ACHU0J_002941"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="ACHU0J_002941"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1217541.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="ACHU0J_002942"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="ACHU0J_002942"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1217542.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="ACHU0J_002943"
     CDS             complement(5841..6881)
                     /locus_tag="ACHU0J_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1217543.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="ACHU0J_002944"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="ACHU0J_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1217544.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="ACHU0J_002945"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="ACHU0J_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1217545.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="ACHU0J_002946"
     CDS             7823..8263
                     /locus_tag="ACHU0J_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1217546.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="ACHU0J_002947"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="ACHU0J_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1217547.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="ACHU0J_002948"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="ACHU0J_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1217548.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11214
                     /gene="rsxC"
                     /locus_tag="ACHU0J_002949"
     CDS             9492..>11214
                     /gene="rsxC"
                     /locus_tag="ACHU0J_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1217549.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQ"
BASE COUNT         2608 a         2887 c         2824 g         2895 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgctcca aagcgcgcaa gctggaacag caac
//
LOCUS       DAZPDE010000123        11069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPDE010000123 DAZPDE010000000
VERSION     DAZPDE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.123"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            111..1010
                     /gene="lysO"
                     /locus_tag="ACHU0J_002950"
     CDS             111..1010
                     /gene="lysO"
                     /locus_tag="ACHU0J_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1217550.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1154..2806
                     /gene="hcp"
                     /locus_tag="ACHU0J_002951"
                     /gene_synonym="priS"
     CDS             1154..2806
                     /gene="hcp"
                     /locus_tag="ACHU0J_002951"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1217551.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2818..3786
                     /gene="hcr"
                     /locus_tag="ACHU0J_002952"
     CDS             2818..3786
                     /gene="hcr"
                     /locus_tag="ACHU0J_002952"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1217552.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3919..5637
                     /gene="poxB"
                     /locus_tag="ACHU0J_002953"
     CDS             3919..5637
                     /gene="poxB"
                     /locus_tag="ACHU0J_002953"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1217553.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5674..6675
                     /gene="ltaE"
                     /locus_tag="ACHU0J_002954"
     CDS             5674..6675
                     /gene="ltaE"
                     /locus_tag="ACHU0J_002954"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1217554.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6686..8116
                     /locus_tag="ACHU0J_002955"
     CDS             6686..8116
                     /locus_tag="ACHU0J_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1217555.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8215..9228
                     /locus_tag="ACHU0J_002956"
     CDS             8215..9228
                     /locus_tag="ACHU0J_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1217556.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9225..10055)
                     /gene="amiD"
                     /locus_tag="ACHU0J_002957"
     CDS             complement(9225..10055)
                     /gene="amiD"
                     /locus_tag="ACHU0J_002957"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1217557.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10052..10375)
                     /locus_tag="ACHU0J_002958"
     CDS             complement(10052..10375)
                     /locus_tag="ACHU0J_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1217558.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10586..>11069)
                     /locus_tag="ACHU0J_002959"
     CDS             complement(10586..>11069)
                     /locus_tag="ACHU0J_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1217559.1"
                     /translation="INCNFFNVDMIRVNLSKCLFHECSMHGVKIKPWLPVKWTKELIS
                     DYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFI
                     YEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH
                     "
BASE COUNT         2549 a         2923 c         2899 g         2698 t
ORIGIN      
        1 ccccctctga tgcataacat ttccttttaa ttgcaacttt actcgccctt ccgctttcag
       61 tagtgtgtca tcgcgcattt cgacttcttc tttgcaagga ctggattttc atgttttctg
      121 ggctgttaat cattctggtt cccctgattg tgggttacct cattccgctt cgccaacaag
      181 ctgcgttaaa agctattaat cagctattaa gctggatggt ttaccttatt ctctttttta
      241 tgggtatcag tctggcgttt ctcgataacc tcgccagtaa cctgttggcg attctgcatt
      301 attctgccgt cagtattacc gttattttac tgtgtaatat tgccgccctg atgtggctgg
      361 agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa actcccgtcg cgtattgcga
      421 tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat tggttttgcc attggtctga
      481 gtggactggc tttcttacaa cacgcgaccg aagccagtga atacacgtta attttgctac
      541 ttttcctcgt tggtattcag ttgcgcaata atggcatgac cttaaagcag attgtcctta
      601 atcgccgggg aatgattgtc gccgtggtgg tggttgtcag ttcattaatt ggtggtttaa
      661 ttaacgcctt tattcttgat ctccccatca ataccgcgct ggcaatggcc tccggtttcg
      721 gctggtattc tctttccggt attttattga ccgaatcttt tggtccggta atcgggagcg
      781 cggcgttttt taatgatctg gcccgtgaac tgattgctat tatgttgatc cctgggctga
      841 ttcgccgcag ccgctctact gcactgggct tatgcggtgc cacatcaatg gatttcaccc
      901 tgcccgttct tcaacgtact ggcgggctgg atatggtccc ggcggcaatt gttcacggtt
      961 ttattcttag cctgttagtg ccgatcctca tcgccttttt ctctgcgtaa tacctctctg
     1021 gcggtagttc cctgccgcca aaattgcgct aaatcaatct cccttaaagt tgcatgaaaa
     1081 atccctttta tccccgcgtt aagcgtctta cccttaaaca tgtatattaa atataacttt
     1141 aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact atccgtactc cggcagaaaa
     1201 cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg gaaacttctg accttcagga
     1261 tttactcatc gcggcgctgc aagggctttc tgcctgggcg gtaaaagcgc gtgaatacgg
     1321 catcatcaac cacgatgttg acagcttcgc gccacgtgcc tttttctcaa ccctgaccaa
     1381 cgttaacttc gattctccgc gtattgtcgg ctacgctcgt gaagcgattg ccttgcgcga
     1441 ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg cgcgtcgata acccgatggc
     1501 tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa cgtcaggcag cagaatttac
     1561 ccctaacaaa gataaagcgg cgattggcga aaacattctc ggcctgcgtc tgctgtgcct
     1621 gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg catgttctcg gtcaatacga
     1681 caacgatatt tatgcccagt accataaaat catggcgtgg ctggggacct ggcctgccga
     1741 tatgaacgcg cttcttgagt gttcaatgga aatcggccag atgaacttca aagtgatgag
     1801 cattctggat gcaggcgaaa ccggtaaata cggtcacccg acgccaaccc aggtcaacgt
     1861 caaagcgacg gcgggtaaat gcattctgat ttccggtcac gatctcaaag atctctacaa
     1921 cctgctggaa cagaccgaag gcacgggcgt taatgtctac acccacggcg aaatgctgcc
     1981 tgcgcatggc tacccggagc tgcgtaaatt caagcatctg gtcggtaact acggcagcgg
     2041 ctggcagaat cagcaagtgg agttcgctcg tttccctggc cccatcgtga tgacctcgaa
     2101 ctgcatcatc gacccaaccg taggcgctta tgacgatcgt atctggaccc gcagcatcgt
     2161 tggctggcct ggcgtgcgtc atctggatgg cgaagatttc tctgcggtca tcgcccaggc
     2221 gcaacaaatg gcgggcttcc cgtacagcga aattcctcac ctgatcaccg tgggctttgg
     2281 tcgccagacg ctgcttggtg cagccgatac gctgattgat ctggttagcc gtgaaaaact
     2341 gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc ggcgagcgcc actacttcac
     2401 cgatttcgcc accagcgtgc cggatgactg cctgatcctg accctcgcct gtggtaaata
     2461 tcgctttaac aaacttgagt ttggcgatat cgaaggtctg ccgcgtctgg tagatgcagg
     2521 tcaatgtaac gatgcttact cagcaattat tctggctgtc actctggcag agaaactggg
     2581 ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc tggtttgaac agaaagcgat
     2641 tgtcattctg ctgacgctgc tttctctggg cgtgaaaaat atcgtcaccg gcccgactgc
     2701 acctggtttc ctgacaccag acctgctggc ggtgctgaac gagaaattcg gcctgcgttc
     2761 tatcaccact gttgaagaag acatgaagca actgttgaac gcgtaaggag tttgtcgatg
     2821 acgatgccaa cgaatcaatg cccgtggcgg atacaggttc atcacattac gcaagaaacg
     2881 ccggatgtgt ggacgatttc cctgatttgc cacgatcact acccatatcg cgccgggcaa
     2941 tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc gtgcttacac catttcctcc
     3001 acgccaggcg tgagtgaata tatcaccctg accgtgcggc ggattgatga cggtgtcggc
     3061 tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc tctggctttc ggacgcgatg
     3121 ggggaattta cctgcgacga taaagcagaa gataaattcc tgttgctggc ggcaggctgc
     3181 ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga agaaccgtcc acaggccgat
     3241 gtgcgggtga tctacaacgt gcgtacgccg caggatgtta ttttcgccga tgagtggcgt
     3301 aactatccgg taacgctggt ggcagaaaat aacgttaccg aaggctttat cgctggtcgc
     3361 ctcactcgcg aactgctggc agatgtacct gacttagctt cacgtaccgt gatgacctgc
     3421 ggcccggctc cgtatatgga ttgggtagag caggaagtga aagcgctcgg cgtgacgcgt
     3481 ttctttaaag agaaattctt caccccagta gcagaagcgg cgaccagcgg tctgaaattc
     3541 accaaactgc aaccggcacg agaattttac gccccggttg gcaccacgct actggaggcg
     3601 ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg cgggtgtttg cggctgctgt
     3661 aagacaaaag tggtttccgg tgaatatacg gtgagcagta caatgacgct gaccgacgcc
     3721 gaaatcgctg aaggttacgt actggcctgc tcctgccatc cgcaggggga tttggttctc
     3781 gcataatcgc cttatgcccg atgatattcc tttcatcggg ctatttaacc gttagtgcct
     3841 cctttctctc ccatcccttc cccctccgtc agatgaacta aacttgttac cgttatcaca
     3901 ttcaggagat ggagaaccat gaaacaaacg gttgcagctt atatcgccaa aacactcgaa
     3961 tcggcaggcg tgaaacgcat ctggggagtc acgggcgact ctctgaacgg tcttagcgac
     4021 agtcttaatc gcatgggcac catcgagtgg atgtccaccc gccatgaaga agtggcggcg
     4081 tttgccgctg gcgctgaagc acaactttca ggagaactgg cggtctgtgc cggatcgtgc
     4141 ggcccaggca acctgcactt gatcaacggc ctattcgatt gccaccgcaa taacgttccg
     4201 gtactggcga ttgccgctca tattccctcc agcgaaattg gcagcggcta tttccaggaa
     4261 acccacccac aagagctatt ccgcgaatgt agtcactatt gcgagctggt ttccagcccg
     4321 gagcagatcc cacaagtgct ggcaattgct atgcgcaaag cggtgcttaa ccgtggcgtt
     4381 tccgttgttg tattaccggg cgacgtggcg ttaaaacctg cgccagaagg ggcaaccacg
     4441 cactggtatc atgcgccaca gccagtcgtg atgccggaag aagaagagtt acgcaaactg
     4501 gcgcaactgc tgcgttattc cagcaatatc gccctgatgt gtggcagcgg ctgcgcgggg
     4561 gcgcataaag agttagttga gtttgccggg aaaattaaag cacctattgt tcatgcccta
     4621 cgcggtaaag agcatgtcga atacgataat ccgtatgatg ttggaatgac cgggttaatc
     4681 ggattctcgt caggtttcca taccatgatg aacgccgaca cgttagtgct actcggtact
     4741 cagttcccct accgtgcgtt ctacccgacc gatgccaaaa ttattcagat tgatattaat
     4801 ccagccagca tcggcgcgca tagcaaggta gatatggcgc tggtcggcga tatcaaatca
     4861 accctgcgcg cattgctgcc actggtggaa gaaaaaaccg atcgcaagtt tctggataaa
     4921 gcgctggaag attaccgcga tgcccgcaaa gggctggacg atttagctaa accgagcgag
     4981 aaagccattc acccgcaata tctggcgcag caaattagtc attttgccgc cgatgacgcc
     5041 atctttacct gtgacgtcgg cacgccaacg gtatgggcgg cacgttatct gaaaatgaac
     5101 ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga tggctaacgc catgccgcag
     5161 gcgttgggtg cgcaggcaac cgagccggaa cgtcaggtgg tcgccatgtg cggcgatggc
     5221 ggttttagca tgttgatggg cgatttcctc tcagtagtgc agatgaaact gccggtgaaa
     5281 attatcgtct ttaataacag cgtgctgggc tttgtggcga tggagatgaa ggccggaggt
     5341 tacctgacag acggtactga gctgcacgac accaactttg cccgcattgc cgaagcgtgc
     5401 ggcattacgg gtatccgtgt agaaaaagcc tctgaagttg atgaagccct gcaacgcgcc
     5461 ttctctatcg acggtccggt gttggtggat gtggtggtcg ccaaagaaga attagccatt
     5521 ccaccgcaga tcaaactcga acaggccaaa ggttttagcc tgtatatgtt gcgcgcaatt
     5581 atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa cgaactggct aaggtaaaaa
     5641 gggtggcatt tcccgtcata ataaggacat gccatgattg atttacgcag tgataccgtt
     5701 acccgaccga gccgcgccat gctcgaagcg atgatggccg ccccggttgg ggacgacgtt
     5761 tacggagacg accctaccgt taatgctctg caggactatg cagcagagct ttccggtaaa
     5821 gaagccgcca tttttctgcc taccggcact caggccaacc tggtcgctct gctcagtcac
     5881 tgcgaacgtg gcgaagagta tattgtcggt caggccgcgc ataactatct gtttgaagcc
     5941 ggtggcgcag cggtgctggg cagtattcag ccgcaaccca tcgacgcggc tgccgacggc
     6001 acgctaccgc tggataaagt ggcgatgaaa atcaaacccg acgatatcca tttcgcccgc
     6061 accaaattac tcagtctgga aaacacccac aacggcaaag tgctgccgcg tgaatacctg
     6121 aaagaagcat gggaatttac ccgcgagcgc aatctggcgc tgcatgttga cggtgcgcgc
     6181 atctttaatg ccgtggtggc ttacggctgc gaactgaaag agatcacgca atattgtgat
     6241 tcgttcacca tttgcctgtc gaaaggtctt gggacgccag tcggttcatt actcgtcggt
     6301 aatcgtgatt acattaaacg tgccatccgc tggcggaaaa tggccggtgg cgggatgcgt
     6361 cagtccggga ttctggctgc cgccgggatg tatgccctgg aaaataacgt cgcacggttg
     6421 caggaagatc acgacaacgc cgcctggatg gcggagcaac tgcgtgaagc aggcgcggat
     6481 gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg gggaagaaaa tgctgccgcg
     6541 ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg cctcgccgat tgtccgcctg
     6601 gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag tcgccgccca ctggcgcgca
     6661 ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg
     6721 ctacattggt cagcatctgg tgcgcacact cagccagcaa gggcatcaga tcctagcggc
     6781 ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca aacgtcagtt gccataaagt
     6841 cgatctcagc tggccggata accttccggc cctgttgcag gatatcgata ccgtctattt
     6901 tctggtgcac agcatgggcg aaggcggcga ttttatcgct caggagcgcc agctggctct
     6961 caacgtccgc gatgcgctac gtgaagtacc agttaagcaa ttaatctttc tcagttcgtt
     7021 gcaggccccg ccacatgagc agtcggatca tctgcgagct cgtcaggcta cggcggacat
     7081 tcttcgtgaa gcgaatgtac cagtgaccga acttcgggcc ggaattatcg ttggcgcagg
     7141 ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac ctgccagtgt taacgccgcc
     7201 acgctgggta cgttcacgca ccacgcccat cgcgctggaa aacttgctgc actatctggt
     7261 ggcgctgtta gaccatccag ccaacgaaca ccgcatcttc gaagccgccg gaccagaggt
     7321 gctcagttat cagcaacagt ttgaacattt tatggcggtg agcggtaagc gccgctggtt
     7381 gatccccatc cccctcccca cccgctggat ttcggtgtgg tttctcaatg tgattacttc
     7441 cgtaccgccc accaccgcca gggcgttgat tcaggggctg aaacacgatc tgctggcaga
     7501 tgataccgcg ctacgtgcac tcatcccaca acggctgatc gctttcgatg acgcggtacg
     7561 tagcacgttg aaagaggaag aaaaactggt caactccagc gactggggct acgacgctca
     7621 ggcctttgcc cgctggcgac cggagtacgg ttatttcgcc aaacaggcgg ggtttaccgt
     7681 taaaacgtcc gccagccttg cggctttatg gctggtggtg aaccaaatcg gcggtaaaga
     7741 gcgttatttc tttggcaata ttttgtggca gacacgggcg ttgatggacc gcgcgatcgg
     7801 tcataatctg gcgaaaggcc gcccggagcg cgaatattta cagaccggcg atgcggtgga
     7861 tagctggaaa gtgattgtcg ttgaaccgaa aaagcaactt acgttgttat ttggcatgaa
     7921 agcgccgggg ctgggacgac tgtgttttac cctggaagat aaaggtgact atcgtactat
     7981 cgatgtccgc gctttctggc atccgcacgg tatgccgggg ctgttttact ggttattgat
     8041 gatccccgcg catctgttta tttttcgcgg aatggcaaaa caaatcgcca gactggcaga
     8101 acaaagcaca gattaatcga caaaacctgc gttattcttt catgtttccc attgcatcat
     8161 gggggaaatc acggaagaat gcgcacgtca ttttctcaac aacagtggat tgatatgaag
     8221 gtactggtta ccggcgccac cagcggctta ggtcgaaacg cggtagagtt tttatgccag
     8281 aaaggcatca gcgtgcgtgc gagcggtcgc aacgaagcaa tgggtaaatt gctggaaaaa
     8341 atgggcgcag agtttgttcc gacggatctg accgagctgg tttcatcaca agctaaagtg
     8401 atgctcgcgg gcattgatac gctgtggcac tgctccagct ttacctcacc ctgggggaca
     8461 caacaggctt tcgatctggc taacgttcgc gccactcgcc gcctgggtga atgggctgtc
     8521 gcctggggtg tgcgtaactt tattcatatc tcttccccct ccctgtactt cgattatcac
     8581 caccatcgca atattaaaga agattttcgc cctcaccgct tcgccaacga gtttgcccgc
     8641 agcaaagcag ccagcgaaga agtgatcaat atgctttcgc aggcgaatcc acaaacgcgc
     8701 tttactattc tgcgcccaca aagtctgttc ggaccgcacg ataaagtctt tattccccgt
     8761 ctggcgcata tgatgcacca ctacggcagt attctgttac cgcatggcgg cagtgcgctg
     8821 gtcgatatga cctactatga aaatgccgtg cacgcaatgt ggctggcaag ccaggaagcc
     8881 tgcgataagc taccttccgg gcgtgtgtac aacatcacca acggcgagca tcgcacactg
     8941 cgcagcatcg tgcagaagct gatcgacgag ttgaatattg actgtcgtat tcgttccgtc
     9001 ccttacccga tgctggatat gatcgcccgc agcatggagc gtttaggccg caagtcagca
     9061 aaagagccgc cgctgaccca ctacggcgcc tccaagttga attttgactt tacgctggat
     9121 attacgcgtg cgcaggaaga gttaggttat cagccggtca tcaccctgga tgaaggcatc
     9181 gagaaaactg ccgcctggct acgcgaccac ggaaaactgc cgcgctaatc ctgcccgtat
     9241 ttctccagca atgcttcggc aatcgcctga gtttctgcat ccgcttcgcc gttatataac
     9301 gtcgggcgga aatgcatctg gaatgccata atcacgcgcc gctgctcgcg cggtgtcata
     9361 tcaggtttta cgtcataacc gtagcgcgcc aaaagctcca gcaatgacgc ggtatctacc
     9421 ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttcaccc gctgcgcatc cggccaggca
     9481 ccaatcccct gctgcgccag ttgctgccag ggaaataatg gccccggatc gtctttgcgc
     9541 tgcggtgcga tatccgcatg tgccactacg ttttccggct tgatgtgata acgggcaata
     9601 atatctttcg ccagcggaat aagcgcctga atctgtgcct gttcaaacgg ggcaaaatat
     9661 ttcactccgg atgatttttg ccagccacgg ttttccagct caatgccaat agaggtgtcg
     9721 ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat gccaggccag ttcttgttcc
     9781 ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg gtggcaccgc agggatcaga
     9841 taatgcgagc tgacctgctt atcagtcagt gtcgccagcg agctatcaaa atcatctgcg
     9901 gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg cctgcgcctg gtgtcgggta
     9961 tcaagctgat atccctcttt ctcgacaatg cctttttcgc ctgcacaccc tgccagtaac
    10021 agagcggcag cgaccagcca gaagactctt ctcatcgacg cgttttcacc gccgtaccgc
    10081 tgacgctaac catcagcata ctgccgtttt gcccgaccgt ttcgtagtcg atatcaatac
    10141 caactacggc atcggccccc agcgcccgcg cctgggagcc taattcctca aaggcgatct
    10201 cccgtgcttt acgcagttct ttttcatacg caccggaacg tccgccaacg atatcgcgga
    10261 taccggcaaa gaaatcacgg aaaatattgg cacctaaaat cgcttcgccg gtcaccacac
    10321 cgcaatattc aacgatggtc aggccttcca gagttggggt tgtagaaaat tgcatgatcg
    10381 atctccttgt ttgttaagcc ttcgatctct aaagcattat cggactgata tgccatgttg
    10441 ggaagaaggt tttgatcagt ttaggaaatc atctcattgt gtcaaaaaca cccacttaag
    10501 tctttacgat tatccgttgc ataacccgat attggtttca ttatagggta ccgcttacct
    10561 gtcttattat gttgcaccaa caacattaat gagcggaaag aggcaactta tattgtaaat
    10621 gactcctaaa gtcttcccca tcttgtgact gaggtctata ccgctggatt gctttttggt
    10681 cagccgccgc aaaacaagaa agtaataaga tctcaatgct tttgtcatta atataagcga
    10741 agatcattct tgcaaactcg tatataaacg cccgatcctg agcgcttaat agtttaatac
    10801 aatatttatt tttaagagag aaaataattt caaggaaagc atccgcagca accttcacgt
    10861 tattcccggc gttcatatta tatatatccc ttgcacagat agagtacaga gatagtaaac
    10921 agccatacaa ataatcactt attaattctt ttgtccattt tacaggcaac cagggtttta
    10981 tcttgacacc atgcatacta cattcatgaa aaagacattt gctgagatta actcttatca
    11041 tatcaacatt gaaaaaattg cagttaata
//
LOCUS       DAZPDE010000124        10878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPDE010000124 DAZPDE010000000
VERSION     DAZPDE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.124"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHU0J_002960"
     CDS             <1..794
                     /locus_tag="ACHU0J_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR1217560.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHU0J_002961"
     CDS             complement(744..1937)
                     /locus_tag="ACHU0J_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217561.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHU0J_002962"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHU0J_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1217562.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHU0J_002963"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHU0J_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1217563.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHU0J_002964"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHU0J_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1217564.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHU0J_002965"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHU0J_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1217565.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHU0J_002966"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHU0J_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1217566.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACHU0J_002967"
     CDS             complement(10573..10716)
                     /locus_tag="ACHU0J_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217567.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
BASE COUNT         2704 a         2748 c         2840 g         2586 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaag
//
LOCUS       DAZPDE010000125        10837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPDE010000125 DAZPDE010000000
VERSION     DAZPDE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.125"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..1114
                     /gene="yghO"
                     /locus_tag="ACHU0J_002968"
                     /pseudo
     CDS             1..1114
                     /gene="yghO"
                     /locus_tag="ACHU0J_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1238..2008)
                     /gene="glcC"
                     /locus_tag="ACHU0J_002969"
     CDS             complement(1238..2008)
                     /gene="glcC"
                     /locus_tag="ACHU0J_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR1217568.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2259..3758
                     /gene="glcD"
                     /locus_tag="ACHU0J_002970"
                     /pseudo
     CDS             2259..3758
                     /gene="glcD"
                     /locus_tag="ACHU0J_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3758..4810
                     /gene="glcE"
                     /locus_tag="ACHU0J_002971"
     CDS             3758..4810
                     /gene="glcE"
                     /locus_tag="ACHU0J_002971"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR1217569.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4821..6040
                     /gene="glcF"
                     /locus_tag="ACHU0J_002972"
                     /pseudo
     CDS             4821..6040
                     /gene="glcF"
                     /locus_tag="ACHU0J_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6045..6449
                     /locus_tag="ACHU0J_002973"
     CDS             6045..6449
                     /locus_tag="ACHU0J_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR1217570.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6471..8642
                     /gene="glcB"
                     /locus_tag="ACHU0J_002974"
     CDS             6471..8642
                     /gene="glcB"
                     /locus_tag="ACHU0J_002974"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR1217571.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8997..10583
                     /locus_tag="ACHU0J_002975"
     CDS             8997..10583
                     /locus_tag="ACHU0J_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR1217572.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2517 a         2715 c         3043 g         2562 t
ORIGIN      
        1 ttgaaaaagt aattaataaa aatgacctta aggcatttat cgcttttccg tcatcacttt
       61 atcccgacga tccaaactgg ataccccctt tattcattga acgcaacgag catttgtctg
      121 cgaaaaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa aaagcggggc
      181 agatagttgg gcgtattacc gcgcaaatag ataccttgca ccgtgagcgt tacggtgaag
      241 ataccggtca tttcggcatg attgacgcca ttgatgatcc gcaggatttt gccgcgttgt
      301 ttggcgcagc ggaagcgtgg ttgaagtcac aaggtgcaag taagatcagc gggcctttca
      361 gcctgaatat caatcaggaa agcggattac tgattgaagg ttttgacaca ccaccctgtg
      421 cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat tgaacaactg ggttatcaca
      481 aaggcattga tttactggcg tggtggatgc agcgaaccga tctcactttc tctccggcgc
      541 taaaaaaact gatggatcag gtgcgcaaaa aggtgaccat tcgctgcatc aatcgtcagc
      601 ggtttgccga agagatgcag atcctgcgtg agattttcaa ctctggctgg cagcacaact
      661 ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgaccaa cttaaatatc
      721 tggtgccgga cgatatgatc tacatcgctg agattgattc tgcaccctgc gcgtttattg
      781 tggcttgcct aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg
      841 ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct
      901 gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt
      961 gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg
     1021 gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta
     1081 caagcgttac cgattgtatg aaaagcaaat ttaataccag atcctgttta gcgcaggggc
     1141 ggtcattcgg gagagggtat agatgaacag atttaacacc agatcctgtt aagtgcaggg
     1201 gcggtcccct cgccttgggg atagggtaaa actctgctca cattcccacc ttgttcatct
     1261 ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg tggagattct
     1321 ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc gacgtgcggc agccgctgca
     1381 acaccgcgtt gtagatccgc gcatgctggc gatcgatctg ctttttttgc tgtggtcgat
     1441 ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc aatgattgca
     1501 gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg aaagcgtgat
     1561 ccaactgcgc atgttcgatc agcgaaatct ctttgtggtt ctcactggcg gcgagcattt
     1621 tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt gccgccagcc
     1681 ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc gttcgcggct
     1741 gcgtactgaa cagatggatc agcgggctgg tgtcctgctc ccgattaagc cgtgcgacat
     1801 gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg gtcagccctt
     1861 cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc gagggaagcg
     1921 gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct gcaaccactt
     1981 cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta ggaccaattt
     2041 ttctgttctt aactcgcaaa acacgcacat cacgcaagtg attatgttac atcaatttaa
     2101 cattaagtta accaagacaa ggtcacagag ctggaaaaaa aatggtctga ccggtagcta
     2161 aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca gcgcgcaaaa
     2221 atcagctgcc acacaacaca acaaagcgaa gcctactcat gagcatcttg tacgaagagc
     2281 gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt actgatggca ctgcgtgagc
     2341 atgtccctgg acttgagatc ctgcataccg atgaggagat cattccttac gagtgtgacg
     2401 ggttgagcgc gtatcgcacg cgtccattac tggttgttct gcctaagcaa atggaacagg
     2461 tgacagcgat tctggctgtc tgccatcgcc tgcgtgtacc ggtggtgacc cgtggtgcag
     2521 gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg gtgatggcgc
     2581 gctttaaaga gagcctcgac attaaccccg ttggtcgccg cgcgcgcgtg cagccgggcg
     2641 tatgtaacct ggcgatctcc taggccgttg cgccgcataa tctctattac gcgccggatc
     2701 cttcctcaca aatcgcctgt tcaattggcg gcaatgtggc ggaaaatgcc ggtggcgtcc
     2761 actgcctgaa atatggtctg accgtacata acctgctgaa aattgaagtg caaacgctgg
     2821 acggcgaggc gctgacgctg ggatcggacg cgctggattc acctggtttt gacctgctgg
     2881 cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg gtaaaactgc
     2941 tgccgaagcc gcccgtggcg cgggttctgt tagccagctt tgactcggta gaaaaagccg
     3001 gacttgcggt tggtgacatc atcgccaatg gcattatccc cggcgggctg gagatgatgg
     3061 ataacctgtc gatccgcgcg gcggaagatt ttattcatgc cggttatccc gtcgacgccg
     3121 aagcgatttt gttatgcgaa ctggacggcg tggagtctga cgtacaggaa gactgcgagc
     3181 gggttaacta catcttgttg aaagcgggcg cgactgacgt ccgtttggca caggacgaag
     3241 cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt cccggcggta ggacgtatct
     3301 ccccggatta ctactgcatg gatggcacca tcccgcgtcg cgccctgcct ggcgtactgg
     3361 aaggcattgc ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc tttcatgccg
     3421 gagatggcaa catgcacccg ttaatccttt tcgatgccaa cgaacctggt gaatttgccc
     3481 gcgcggaaga gctgggcggg aagatcctcg aactctgcgt tgaagttggc ggcagcatca
     3541 gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag ttcaacagcg
     3601 atgaaatcac gaccttccat gcggtcaagg cggcgtttga ccccgatggt ttgctgaacc
     3661 ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt tggtgccatg catgtgcatc
     3721 acggtcattt accttttcct gaactggagc gcttttgatg ctacgcgagt gtgattacag
     3781 tcaggcgctg ctggagcagg tgaatcaggc aattagcgat aaaacgccgc tggtgattca
     3841 gggcagcaat agcaaagcct ttttaggtcg cccagtcacc gggcaaacgc tggatgtgcg
     3901 ttgtcatcgc ggcattgtta attacgaccc gaccgagctg gtgataaccg ctcgtgccgg
     3961 aacgccgctg gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc ttccctgcga
     4021 gccgccgcat tatggtgaag aagccacctg gggcgggatg gtcgcctgcg ggctggcggg
     4081 gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc gcatcattac
     4141 cggcactgga aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg ccggatacga
     4201 tctctcacgg ttaatggccg gaagctacgg ttgtcttggc gtgctcactg aaatctcaat
     4261 gaaagtgtta ccgcgaccgc gtgcctctct gagcctgcgt cgggaaatca gcctgcaaga
     4321 agccatgagt gaaatcgccg agtggcaact ccagccatta cccattagtg gcttatgtta
     4381 cttcgacaat gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa aagcagcgcg
     4441 tgaactgctg ggtggcgaag aggttgtggg tcagttctgg cagcaattgc gtgaacaaca
     4501 actgccgttc ttctcgttac caggtacctt atggcgcatt tcattaccca gcgatgcgcc
     4561 gatgatggat ttacccggcg agcaactgat cgactggggc ggggcgttac gctggctgaa
     4621 atcgacagcc gatgacaatc aaatccatcg catcgcccgc aacgctggcg gtcatgcgac
     4681 ccgctttagt gccggagatg gtggctttgc cccgctatcg gctcctttat tccgctatca
     4741 ccagcagctt aaacagcagc tcgacccttg cggcgtgttt aaccccggtc gcatgtacgc
     4801 ggaactttga ggagcaggct atgcaaaccc gattaaccga agagatgcgg cagaacgcgc
     4861 gctggaagcc gacagcatcc tgtgcgcctg tgttcactgc ggattttgta ccgcaacctg
     4921 cccaacctat cagcttctgg gcgatgaact ggacgggccg cgcgggcgca tctatctgat
     4981 taaacaggtg ctggaaggca acgaagtcac gcttaaaaca caggagcatc tcgatcgctg
     5041 cctcacttgc cgcaattgtg aaaccacctg tccttctggt gtgcgctatc acaatttgct
     5101 ggatatcggg cgtgatattg tcgagcagaa agtgaaacgc ccactgccgg agcgaatgct
     5161 gcgcgaagga ttgcgccagg tagtgccgcg tccggcggtc ttccgtgcgc tgacgcaggt
     5221 agggctggtg ctgcgaccgt ttttaccgga acaggtcaga gcaaaactgc ctgctgaaac
     5281 ggtgaaagct aaaccgcgtc cgccgctgcg ccataagcgt cgggttttaa tgctggaagg
     5341 ctgcgcccag cctacgcttt cgcccaacac caacgcggca actgcgcgag tgctggatcg
     5401 tctggggatc agcgtcatgt cagctaacga agcaggctgt tgtggcgcgg tggactatca
     5461 tcttaatgcg caggagaaag ggttggcacg ggcgcgcaat aatattgatg cctggtggcc
     5521 cgcgattgaa gcaggtgccg aggcaatttt gcaaaccgcc agcggttgcg gcgcgttcgt
     5581 caaagagtat gggcagatgc tgaaaaacga tgcgttatat gccgataaag cgcgtcaggt
     5641 cagtgaactg gcggtcgatt tagtcgaact tctgcgcgag gaaccgctgg aaaaactggc
     5701 aattcgcggc gataaaaaac tggccttcca ctgcccgtgt accctacaac atgcgcaaaa
     5761 gctgaacggc gaagtggaaa aagtgttact tcgtcttgga tttaccttaa cggacgttcc
     5821 cgacagccat ctgtgctgcg gttcagcggg aacatatgcg ttaacgcatc ccgatctggc
     5881 acgccagctg cgggataaca aaatgaatgc gctggaaagc ggcaaaccgg aaatgatcgt
     5941 caccgccaac attggttgcc agacgcatct ggcgagcgcc ggtcgtacct ctgtgcgtca
     6001 ctggattgaa attgtagaac aagcccttga aaaggaataa aaaaatgaaa actaaagtca
     6061 ttcttagcca gcaaatggcg agtgcaatta ttgccgcagg tcaggaagag gcgcagaaaa
     6121 ataactggtc tgtttccatt gctgttgccg atgacggcgg tcatctgctg gcgttaagtc
     6181 gcatggacga ttgcgcgccg attgcggctt acatctccca ggagaaagcg cgtaccgccg
     6241 cgctggggcg tcgtgaaact aagggctatg aagagatggt gaacaacgga cgtaccgcgt
     6301 tcgtgactgc gccgttactg acgtcgctgg aaggcggcgt accggttgtt gttgatgggc
     6361 aaattattgg tgccgtgggc gtttctggtt taaccggagc acaggatgcg caggtcgcga
     6421 aagtggcagc agcggtgttg gcgaaataag cgaaaacgag gagagaaaca atgagtcaaa
     6481 ccataaccca gggccgttta cgcattgacg ccaattttaa acgttttgtg gatggagaag
     6541 ttttgccggg cgtggaactg gacgctgctg cattctggca caatgttgat gagatcgttc
     6601 acgatctggc accagaaaat cgtcagttgc tggcagaacg cgatcgcatt caggcggcgc
     6661 ttgatgagtg gcatcgcagc aatccggggc cggtaaaaga taaagcggcc tataaatctt
     6721 tctttcgtga actgggctac ctggtgccgc aaccggagcg cgtgacggtg gaaaccacgg
     6781 gcattgacag cgaaatcacc agtcaggcgg ggccacagct ggtggttccg gcaatgaacg
     6841 cccgctacgc gctgaacgcg gcgaacgctc gctggggctc actgtacgat gcgttatacg
     6901 gcagcgacat catcccgcag gaaggggcga tggtcagtgg ctacgatccg cagcgcggtg
     6961 agcaggttat cgcctgggtt cgacgtttcc tcgatgaatc actaccgctg gaaaacggca
     7021 gctatcagga tgtggtggcg tttaaggtgg tcgataaaca attacgcatc cagttgaaaa
     7081 atggtaaaga aaccacgtta cgtactccag cacagtttgt cggttaccgt ggcgatgccg
     7141 ctgcgccgac ctgcattttg ctgaaaaata acggcctgca tattgaactg caaattgatg
     7201 ccaacgggcg gattggcaaa gacgatccgg cgcacatcaa cgatgttatc gtcgaagctg
     7261 ctatcagtac cattctcgac tgcgaagatt cggtcgcggc ggttgatgcg gaagataaaa
     7321 tcctgctgta ccgcaatctg ctgggcctga tgcaggggac tctgcaagag aaaatggaga
     7381 aaaacggtcg gcaaatcgta cgtaaactga atgacgatcg tcagtacacc gccgccgatg
     7441 gctctgaaat ttctctgcac ggacgctcgt tgctgtttat ccgcaacgtg ggtcatttga
     7501 tgaccattcc tgtgatttgg gacagcgaag gcaatgaaat cccggaaggt attcttgatg
     7561 gcgtcatgac tggcgcgatt gccctctatg atttaaaagt gcagaaaaac tcgcgcactg
     7621 gcagcgtcta tattgtgaaa ccgaaaatgc acggtccgca ggaagtggcg ttcgccaaca
     7681 aactgtttac ccgcattgag acaatgctcg gtatggcacc gaataccctg aaaatgggca
     7741 ttatggatga agaacgccgg acctcgctga acttgcgtag ctgtatcgct caggcgcgca
     7801 accgcgtggc gttcatcaat accggtttcc tcgaccgtac cggcgatgaa atgcattcgg
     7861 tgatggaagc tggcccgatg ctgcgtaaaa accagatgaa atcgacgccg tggatcaaag
     7921 cctacgaacg taataacgtg ctttccggtc tgttctgtgg gctgcgcggt aaagcgcaaa
     7981 tcggtaaagg catgtgggca atgccggacc tgatggcaga catgtacagc cagaagggcg
     8041 accaactgcg tgccggggca aacacagcct gggttccgtc accaaccgct gctacgctcc
     8101 atgcgctgca ctaccaccat accaacgtac agagcgtgca agccaacatt gcccagaccg
     8161 agttcagtgc tgaatttgaa ccgctgctgg acgatctgct gactattccg gtggctgaaa
     8221 acgctaactg gtcggcgcaa gagatccaac aagagctgga taacaacgtg caggggattc
     8281 tggggtacgt ggtgcgttgg gtggagcagg ggattggttg ttcaaaagtg ccggatattc
     8341 acaacgtggc gctgatggaa gaccgcgcaa cgctgcgtat ctccagccag catatcgcca
     8401 actggttacg tcacggtatt ctgaccaaag aacaggtgca ggcgtcgctg gagaatatgg
     8461 cgaaagtggt tgatcagcaa aatgctggcg atccggctta tcgtccgatg gcggggaatt
     8521 tcgctaactc gtgtgctttt aaagctgcca gcgatttaat cttcctcggc gtgaaacagc
     8581 caaacggtta taccgaaccg ttattacacg cctggcgttt acgcgaaaaa gaaagtcatt
     8641 aatagcgtca aaaaagcccc ggcaagtttt attgtcgggg ctatgatagt aactgcggta
     8701 gtacatttat cccggaaata accggagcga ttatcagggt ttaacaatat taaatacccg
     8761 tagcgcatta ctagcgggcc taaaacaaca atatggacat cttaacgata ctctgcgcct
     8821 gcgtgtgcgg aacagaaacc cttacccgat atacttgtta tgccagaggt agcaattgcg
     8881 ttagcggcgt actgttgctg taaagtataa cgcctatatt ttacagttca gtcctctttt
     8941 gcttattaca accttaagta tatcaagcat ataaagataa taagagactg aacaatatgg
     9001 ttacctggac ccaaatgtat atgccgatgg gaggactggg gctatccgct ctggtcgccc
     9061 tgatcccgat aatattcttc ttcgttgcac tcgcggtatt acgtctgaaa ggacatgttg
     9121 ctggagcaat aacccttata ttatctatcc tgattgcgat attcgccttt aaaatgccga
     9181 ttgatatggc atttgctgct gcgggctatg gctttattta tggattatgg ccaattgcgt
     9241 ggattattgt cgcggcggtg ttcctgtata aattaaccgt tgccagcggg cagttcgata
     9301 ttatccgcag ctcggttatc tccatcaccg acgatcagcg tttgcaggtg ttactgattg
     9361 gtttctcctt tggtgcgttg ctggaaggag ctgctggctt tggtgcgacg gtggcgtttg
     9421 gtgcgttggg cgtgccgatt ctggtcgccg gtcaggtaac gggaatcgat ccgttccaca
     9481 ttggcgcaat ggcgggacgt cagttaccgt tcctgtcggt tcttgtgccg ttctggctgg
     9541 tagcaatgat ggacggctgg aaaggggtga aagagacgtg gccagcggcg ctggttgctg
     9601 ggggaagctt cgctgtcact cagttcttta cctctaacta tattggtccg gaactgccgg
     9661 atattacttc ggcgctggtg agtatcgtct cactcgcttt attccttaaa gtctggcggc
     9721 cgaaaaatac cgaaacggca atcagcatgg ggcaatccgc aggtgcgatg gtggtaaata
     9781 agccatcttc tggcggtccc gtgccttcag aatatagtct ggggcaaatc attcgtgcgt
     9841 ggtcaccgtt tttaatctta acggtgctgg tcaccatctg gaccatgaag ccgtttaaag
     9901 cgttatttgc tccgggcggc gcgttttatt cactggtgat taatttccag atccctcatt
     9961 tgcatcaaca agtgttgaaa gcggcaccca ttgtcgccca accaacgcca atggatgcgg
    10021 tgtttaaatt cgaccctctc tcggctggcg gcaccgctat ttttattgcg gcgattatct
    10081 ctatcttcat cctcggcgtg ggcatcaaga aagggattgg cgtctttgcc gaaatgctaa
    10141 ttagtctgaa gtggccgata ttgtcgattg gcatggtgct ggcattcgcc ttcgtcacca
    10201 actattccgg catgtccacc acgctggcgc tggtactggc aggtacaggc gtgatgttcc
    10261 cgtttttctc accgtttctc ggctggctgg gcgtattcct taccgggtcg gacacttctt
    10321 caaacgccct gtttggttca ctgcaatcga ccacggcgca gcaaatcaac gtctctgaca
    10381 ctctgctggt ggcagcaaat accagcggcg gcgtaactgg caagatgatc tccccgcaat
    10441 ctatcgccgt ggcctgcgcc gcgacgggca tggtgggccg agaatctgaa ctgttccgct
    10501 acaccgtgaa gcacagtctg atttttgcca gcgttatcac cctgctgcag gcgtatgtgt
    10561 ttaccgggat gttagtctcg taaatcaata aagccggatg aaggtaaata tcacccggct
    10621 ttccatttgt ctgtcgttta tctcgccagg gaaccgtttt tacgattggc ttctttatac
    10681 gtagtctccg ttttacgatg cgaattgttt aatatttata caacttggct tctgtgctgg
    10741 ttataaagta cccactttca tgagatcaat tttagtatgt tattacattg cttcatctgt
    10801 tgctttcatg acgaatggcg ttttttctct tatttat
//
LOCUS       DAZPDE010000126        10835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPDE010000126 DAZPDE010000000
VERSION     DAZPDE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.126"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..895
                     /locus_tag="ACHU0J_002976"
     CDS             <1..895
                     /locus_tag="ACHU0J_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1217573.1"
                     /translation="ELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGA
                     RLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPF
                     IRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVI
                     VHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            1030..2250
                     /gene="mlc"
                     /locus_tag="ACHU0J_002977"
                     /gene_synonym="dgsA"
     CDS             1030..2250
                     /gene="mlc"
                     /locus_tag="ACHU0J_002977"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1217574.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2375..3070
                     /gene="bioD"
                     /locus_tag="ACHU0J_002978"
     CDS             2375..3070
                     /gene="bioD"
                     /locus_tag="ACHU0J_002978"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1217575.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3023..4315)
                     /gene="clcB"
                     /locus_tag="ACHU0J_002979"
     CDS             complement(3023..4315)
                     /gene="clcB"
                     /locus_tag="ACHU0J_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1217576.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="ACHU0J_002980"
     CDS             complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="ACHU0J_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1217577.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5131..5985)
                     /gene="ynfH"
                     /locus_tag="ACHU0J_002981"
     CDS             complement(5131..5985)
                     /gene="ynfH"
                     /locus_tag="ACHU0J_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1217578.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5987..6604)
                     /gene="ynfG"
                     /locus_tag="ACHU0J_002982"
     CDS             complement(5987..6604)
                     /gene="ynfG"
                     /locus_tag="ACHU0J_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1217579.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6615..8653)
                     /locus_tag="ACHU0J_002983"
                     /pseudo
     CDS             complement(6615..8653)
                     /locus_tag="ACHU0J_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(8714..9748)
                     /locus_tag="ACHU0J_002984"
                     /pseudo
     CDS             complement(8714..9748)
                     /locus_tag="ACHU0J_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            9806..>10835
                     /locus_tag="ACHU0J_002985"
     CDS             9806..>10835
                     /locus_tag="ACHU0J_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1217580.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT         2673 a         2766 c         2810 g         2586 t
ORIGIN      
        1 tgaacttcgt catctgcgtt actttgttgc tgttgcggaa gagctgcatt ttgggcgcgc
       61 ggctgcccgc ctgaatattt cgcaaccgcc gctaagtcag cagattcagg cactggagca
      121 acaaatcggt gcccgactgc tggcacgaac taatcgcagt gtattgctga cggcagcagg
      181 aaaacagttt cttgcagata gtcggcaaat cctgtctatg gtggatgacg ctgccgctcg
      241 cgctgaaaga ctgcatcagg gtgaagcggg ggagttgcgt attggtttta cttcgtccgc
      301 tccttttatt cgggcggtgt ccgatacgtt atcgctgttt cgccgtgatt atcctgatgt
      361 ccatttacaa acccgcgaaa tgaacactcg cgagcaaatc gctccgctca ttgaaggaac
      421 gctggatatg ggattgctgc gtaacacagc gttaccggag acgcttgaac acgcagtcat
      481 cgtccatgaa ccgcttatgg cgatgatccc gtacgatcat cccctggcaa ataatccgag
      541 tgtaacactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt ttgatccgca
      601 cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc atttgacgcc
      661 cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt ccgccggtct
      721 gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa tgcgctgggt
      781 gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga
      841 acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa
      901 atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac
      961 gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg
     1021 gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa gcagaccaac
     1081 gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc
     1141 cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat gctcgaagca
     1201 cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg
     1261 ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt
     1321 ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg
     1381 ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca gttttttatc
     1441 cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc gggaattatt
     1501 gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg
     1561 ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca tgatatcagc
     1621 gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag
     1681 gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct gctacacgca
     1741 ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg taaacgctgt
     1801 tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat tcttgagctg
     1861 gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc gttaaccgtg
     1921 gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat cattatcggg
     1981 gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa cccacaaaaa
     2041 atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt catctcagac
     2101 agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag
     2161 ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat gtataacggt
     2221 tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg
     2281 ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctag
     2341 tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct ttattaccgg
     2401 tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc
     2461 ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga
     2521 agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg aactgcctta
     2581 tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc
     2641 aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag tcgatcatgt
     2701 cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc cactctctga
     2761 atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg aaggttgcat
     2821 taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc tcattggctg
     2881 ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg atgtgctggg
     2941 taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg
     3001 tgaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg tagatagagt
     3061 cacggtgtaa cgtccgcgaa attaccgacg caattacgca ggcaatcaat aaaccgggga
     3121 gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc ggcgcgtgcg
     3181 tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc
     3241 catcagggaa ccataatccc aggctacgac catacaacat gccaatggca agaccgataa
     3301 atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc agcacggcac
     3361 acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg gttaaaaagg
     3421 attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agcagaccca
     3481 cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc acaaatccac
     3541 gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc agcacacctg
     3601 tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt acgttgtaga
     3661 gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg gcggaaataa
     3721 tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac
     3781 tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca caggcgatcc
     3841 ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag
     3901 ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagcg
     3961 aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt
     4021 ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag cccatcagca
     4081 acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag gaggaaaggt
     4141 ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac tccagtagca
     4201 gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg actgttgcga
     4261 taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca
     4321 gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca
     4381 gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc gaatcgtgcg
     4441 taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt taaccgcgac
     4501 aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt cacccagtgc
     4561 acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg
     4621 aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca
     4681 cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcaaa
     4741 ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac
     4801 agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc acgggccgac
     4861 aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac
     4921 cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt cactggtgag
     4981 taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca gcgcgcccaa
     5041 cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct
     5101 gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc ctactgtcat
     5161 gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga ggattagccc
     5221 cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa gccagcatcc
     5281 aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat ctggtaccag
     5341 tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt gcagcacaat
     5401 cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg tggtattaaa
     5461 tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca acaataccgt
     5521 caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata
     5581 cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag
     5641 tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata gtccgccaac
     5701 ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc cgattcgatt
     5761 cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa taaacccgat
     5821 gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt
     5881 tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc attgacccaa
     5941 tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac acttcctccg
     6001 gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta ggtttgataa
     6061 cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc agagtgccgt
     6121 gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg caggattcga
     6181 cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt
     6241 gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc
     6301 agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta tgcattgcgc
     6361 cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag atggagagat
     6421 aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg ccgccagcat
     6481 attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct ttgcacgcca
     6541 gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg
     6601 tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga cgggtttccc
     6661 tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc gtgatcgacc
     6721 cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt aacgccaggc
     6781 agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt aaatacccgc
     6841 acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat ccacacctct
     6901 tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt acgcgctttg
     6961 gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt gctgaggttg
     7021 cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag ataaggtctt
     7081 cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc gaggccgtca
     7141 tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc acctcatggg
     7201 tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac cataaaaact
     7261 tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg gtcattttcg
     7321 taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta acaggattct
     7381 cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca cctgagttgc
     7441 cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg cgggacgttt
     7501 gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca ggttttgctg
     7561 aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg atttttgctg
     7621 cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag gctttataat
     7681 gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca caatatttgt
     7741 cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc gcacaggcca
     7801 gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg gcagctgtgt
     7861 cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg gcttgctcga
     7921 cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt ccgaacataa
     7981 cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca ccgaacatat
     8041 aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg agaaaacccc
     8101 cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg ccatctacgc
     8161 ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc cgccgaagat
     8221 tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta ccttcaccgc
     8281 gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc cgtcggcgga
     8341 tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg tcacctgtcg
     8401 tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc agacagcggc
     8461 tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga gcctctgccg
     8521 cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg agactgccaa
     8581 gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc gcctctgtgg
     8641 tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg ccagaattta
     8701 tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc gggtttccct
     8761 tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca tggtcgattt
     8821 tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta actccaggga
     8881 gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta aacacccgca
     8941 ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc cacacctcct
     9001 gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta cgggatttgt
     9061 aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc cagccttcaa
     9121 aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt tcccaggtac
     9181 gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc gttttaagtg
     9241 gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca ttgggatctt
     9301 tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc ggatctttgg
     9361 caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct tctgtgaact
     9421 tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag taaatcggtt
     9481 tacgttcgaa tttttcgctg gtgactggct ggaggaaaat gagatatccc atgttaccgg
     9541 cgtagtcgtt aggaataata tcttcctgct caacggtcat caggtcttcc agcagaatgt
     9601 cggcatattt tgccgatgag gtcataaagt tttcgatgac cacaatcatt tcgcatttcg
     9661 attcgtcctg cagaatttca tgcgttttgt tgatgtcaga atgctgatta acgagggtat
     9721 ttcccgcgta gttccagatg gactccattg ccagacggat ggatggactt gaagtagttg
     9781 actcaatgct acttaaggtc ctgatatgac tgacattaat catgataaca ctgtatatcc
     9841 atccagttct ttctctgggt ctccctatcg gtatcagcaa gtctacacct caagccctga
     9901 cacaaggctg acacaaatca atctactcag cgtagctaag cctcagttag tccgcagagc
     9961 caatggtcga tatactatcc gcttcagact caagggacag accacaccgt ttctttcagt
    10021 atcgacccgc agcacagaca ggagagtagc cacaatgcgt cagagagagc ttgcagccac
    10081 cgctaaggcc ttcatgttgg ataggcctga agtctcacta caggagctta cagagcatct
    10141 ccggtctatg gctgagcagt tcctgacaga cgctagtgac gattactgga atggacttga
    10201 ggtcgctact ctggtcgacg agaagtccaa cctgaaggag ctagccgcta cgcaagcctt
    10261 gagtctggac cagcagaagg gcataaggtt agcgctggaa gtcctgaccg cagcacagca
    10321 gagagtcgac actggtgata cctctggtct gattaagctg attgatgata ataaccttac
    10381 tgatgactca actataggcg actctacgtc aattctgaat aatgagcagg gggatagacc
    10441 ggcagttttc acacaggagc gccagtcatc tgtagttttt tcaagcctag tgtcctcgct
    10501 gctggctgag aaggtccaga ccctgaagac atccagctac aaggacctct cgtcctcact
    10561 caatactgtc tcccggttcc tgcctgaaga catggacctg atgtcccgct ctgggtggct
    10621 ggctgtcagg gactctatgc tggcttctga ggtgagacca tcgacaatca acaagctgct
    10681 gaccaaagcg aagatgtgtc tcgattatgg cctgatgaat gggcaacttg agggccgcaa
    10741 ccctattgaa cggatgaagc tgaccaagga tatcgactcc aagcgcagag cgttcacaga
    10801 cgaggagctg gagagacttc tggtccgtgt tgagt
//
LOCUS       DAZPDE010000127        10788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPDE010000127 DAZPDE010000000
VERSION     DAZPDE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.127"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            151..1551
                     /locus_tag="ACHU0J_002986"
     CDS             151..1551
                     /locus_tag="ACHU0J_002986"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1217581.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1707..3242
                     /gene="gadC"
                     /locus_tag="ACHU0J_002987"
     CDS             1707..3242
                     /gene="gadC"
                     /locus_tag="ACHU0J_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1217582.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3373..4692
                     /locus_tag="ACHU0J_002988"
     CDS             3373..4692
                     /locus_tag="ACHU0J_002988"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1217583.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5069..6139
                     /gene="dosC"
                     /locus_tag="ACHU0J_002989"
                     /pseudo
     CDS             5069..6139
                     /gene="dosC"
                     /locus_tag="ACHU0J_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            6139..6756
                     /locus_tag="ACHU0J_002990"
                     /pseudo
     CDS             6139..6756
                     /locus_tag="ACHU0J_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            7014..7346
                     /gene="ddpX"
                     /locus_tag="ACHU0J_002991"
                     /pseudo
     CDS             7014..7346
                     /gene="ddpX"
                     /locus_tag="ACHU0J_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7360..8256
                     /gene="ddpC"
                     /locus_tag="ACHU0J_002992"
     CDS             7360..8256
                     /gene="ddpC"
                     /locus_tag="ACHU0J_002992"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1217584.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8253..9227
                     /locus_tag="ACHU0J_002993"
     CDS             8253..9227
                     /locus_tag="ACHU0J_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1217585.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9220..10146
                     /locus_tag="ACHU0J_002994"
                     /pseudo
     CDS             9220..10146
                     /locus_tag="ACHU0J_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(10202..10633)
                     /gene="osmC"
                     /locus_tag="ACHU0J_002995"
     CDS             complement(10202..10633)
                     /gene="osmC"
                     /locus_tag="ACHU0J_002995"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1217586.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2633 a         2654 c         2712 g         2789 t
ORIGIN      
        1 ttgtttttaa tgaaaaaata tcattccaac aatatctcac gataaataac attaggattt
       61 tgttatttaa acacgagtcc tttgcacttg cttactttat cgataaatcc tactttttta
      121 atgcgatcca atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg
      181 tcggaactac tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa
      241 cgttttccgc tgcacgaaat gcgcgacgat gtcgcatttc agattatcaa tgatgaatta
      301 tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa
      361 aacgtccaca agttgatgga tttatccatt aacaaaaact ggatcgacaa agaagaatat
      421 ccgcaatccg cagccatcga cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg
      481 cctgcgccga aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt
      541 atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa
      601 ccaacggata aaccaaacct ggtgtgcggt ccggtgcaaa tctgctggca taaattcgcc
      661 cgctactggg atgtggagct gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac
      721 ccgaaacgca tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc gactttcggc
      781 gtgacctaca ccggtaacta tgagttcccg caaccgctgc acgatgcact ggataaattc
      841 caggccgaca ccggtatcga catcgacatg cacatcgacg ccgccagcgg tggctttctg
      901 gcaccgttcg tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc
      961 agtgcttcag gccataaatt cggtctggct ccgctgggct gcggctgggt tatctggcgt
     1021 gacgaagaag cgctgccgca ggaactggtg ttcaacgttg actacctcgg tggtcagatt
     1081 ggtacttttg ccatcaactt ctcccgcccg gcgggtcagg tgattgcaca gtactatgaa
     1141 ttcctgcgcc ttggtcgtga aggctatacc aaagtacaga atgcttccta ccaggtcgct
     1201 gcctatctgg cggatgagat cgccaaactg gggccgtatg agttcatctg taccggtcgc
     1261 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccgggatac
     1321 accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
     1381 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa
     1441 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgat
     1501 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacatacctg ataacgttta
     1561 acggtaacgg tgtcccgaaa cgaacccgtt tcgggataat ttccaaagtc tgttcactgg
     1621 cattagcaac ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga
     1681 caggaatacc gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc
     1741 agctcacatt actcggattc tttgccataa cggcatcgat ggtaatggct gtttatgaat
     1801 accctacctt cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt
     1861 tatggtttat tcccgtggga ctttgtgctg cggaaatggc caccgtcgac ggctgggaag
     1921 aaggtggtgt cttcgcctgg gtatcaaata ctctgggtcc gagatgggga tttgcagcga
     1981 tctcatttgg ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag
     2041 gggcactctc ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta
     2101 ttgcagcact catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca
     2161 cggcgcgaat tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga
     2221 tcgcattagc ggctatttac ctgcactccg gtgcccccgt tgctatcgaa atggattcga
     2281 agaccttctt ccctgacttc tctaaagtgg gcacactggt tgtatttgtt gccttcattt
     2341 tgagttatat gggcgtagaa gcttccgcaa ctcacgtcaa tgaaatgagc aaccccgggc
     2401 gcgactatcc actggctatg ttactgctga tggtggcggc aatctgctta agctctgttg
     2461 gcggtttgtc tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa
     2521 tgcaaacctt taccgttctg atgtcccatg tggcaccgga aattgagtgg acggttcgcg
     2581 tgatctccgc actgctgttg ctgggtgttc tggcggaaat cgcctcctgg attgttggtc
     2641 cttctcgcgg gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa
     2701 tgaacaaaaa tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acttctatcg
     2761 cgttgatcat cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg
     2821 cgctgacggt ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt
     2881 tggttcttaa acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga
     2941 aactggtcgt ggcaattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc
     3001 tgccgccgga taacatccag ggtgattcta ccgatatgta tgttgaatta ctggttgtta
     3061 gtttcctggt ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaggca
     3121 aagcgaatac cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact
     3181 tcttcctgca cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact
     3241 aaccatgtct gaatggttat aagacaaggg agcgataatt catcgctccc tttttcgtgc
     3301 ttgctgtcgt attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg
     3361 aaaccagcac atatggatat ctgctcccga aacaagaaat taacgattag aagaccagcg
     3421 atactggttg cattggcact tttactgtgt agttgtaaaa gcacgcctcc agagtccatg
     3481 gtgacaccac cagcaggttc aaagccacca gccacgacgc aacaatcgtc acaaccgatg
     3541 cgtggcatct ggctggccac ggtttctcgc ctcgactggc caccggtttc ctcggttaac
     3601 attagtaatc ccaccagccg agcccgtgta caacaacagg cgatgatcga caaactggat
     3661 catctccaac gtctcggcat aaacacggtc tttttccagg tcaagccgga cggtaccgcc
     3721 ctgtggccat cgaaaatttt gccgtggtcc gatcttatga ccggtaagat tggtgaaaat
     3781 ccgggttacg atccgctgca attcatgctc gacgaagccc acaagcgtgg gatgaaagta
     3841 cacgcctggt ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa
     3901 ctgaatagca ctctgtctca acaaccggcg agcgtctatg tgcaacaccg tgactggatc
     3961 agaacgtctg gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca ggactggatc
     4021 acatcaatag tcgctgaagt ggtttcccgc tatccggtag atggcgtgca gtttgacgac
     4081 tatttctata ctgaatcacc gggttcacgg ctaaatgata acgaaacgta ccgtaaatac
     4141 ggaggcgcat ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca
     4201 aaggtatcac acaccattaa aagcattaag ccgggagtcg aatttggcgt tagcccggca
     4261 ggcgtgtggc gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc
     4321 tatgacgaat cctacgcaga cacccgtcga tgggtggaac aaggattgct ggattacatt
     4381 gctccccaaa tttactggcc gttctcacgg agtgccgcgc gttatgacgt gttggcaaaa
     4441 tggtgggcgg atgtcgttaa accaaccagg acccgcctgt atatcggtat cgccttctat
     4501 aaagtgggtg aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa
     4561 ctgaaaaagc agctcgatct taacgatgcg gtaccagaaa ttagcggcac catcttgttc
     4621 cgtgaggact atctgaataa accgcagact caacaagcgg tcagctatct gcaaagtcgt
     4681 tggggcagtt aaactaccat gcttactttg taacaagccg gtgctttacc caccggcttg
     4741 ttacaccttg tgaaatatcc cactagtaat catgcttaca taagtcaaat taaccacgca
     4801 attaaccaca cttagttaaa cagctataaa tccctaataa aaatgtatga atatgaaaaa
     4861 ccatacgata ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac
     4921 agtagcgtag attgaaactg tcttaagtcg cttatttctt ttcagtatat cttcatattt
     4981 caggagaata tggtgcagtt agtcgatctc gcacgttgcg tttcgttttg attccgtctg
     5041 atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa tgaaagatga
     5101 gtggaccgga cttgtcgaac aggcagatcc tctcattcgt gctaaagccg cagaaattgc
     5161 ccttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca tcgacccgca
     5221 tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg
     5281 ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca
     5341 gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg tcgagatggg
     5401 gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt attccgccgc
     5461 agaaaaactt caggtctacc atttctcgat taacagtatt gatatcgcga tggaagtgat
     5521 gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa actatcgtat
     5581 cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct caatactttc
     5641 atgggaaata gatattatct ataaagtcct gctggattct gatttaggca gcagtttgcc
     5701 tttaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt attttagcgg
     5761 cattgccgaa gtgggccata tctcccgtct gattcaggat ttcgacggta ttttcaatca
     5821 aaccatgcgt aacaccagga ttttgaataa cagaagtttg cgggtgaaat ttctattaca
     5881 gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg aagaagtatc
     5941 gcgccacgaa gtcggtatgg atgtactgac gaaattactt aaccgccgtt tcctgccgac
     6001 tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt cagtgctgat
     6061 tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca ctggcgatga
     6121 aattctgcgt aaagtctcat tcctctcgca gaagagattg gtgaaatcga aaatattggg
     6181 cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat
     6241 atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat
     6301 caggtgtctg atgcaatgca agcctggggt attgacggcc accagctgac ggtggaaatc
     6361 acggaaagca tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgt
     6421 gatatgggcg tgggtttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc
     6481 ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa gctttgtcga tcgttgtctg
     6541 acggaaaaac gcatcctggc cttacttgaa gccattacca gcattgggca aagcctcaat
     6601 ttaaccgtcg tggcggaagg ggtcgaaacc aaagagcaat ttgagatgct acgtaagatc
     6661 cactgtcgcg ttattcaggg atatttcttt tcccgccccc ttcccgccga agaaattcca
     6721 ggctggatga gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc
     6781 gggttttcct ttaaggtgac actgtcactt aaatactgtg atttcagcta cgattccggg
     6841 agattccttc cttaactttc cctcttgcac caaaactgca ctacgctgca caaatgttga
     6901 tcaaaagtta aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg
     6961 agatctgcat aagcggaaag cgcagcaatt tttgtcttat acaggaaccg tttatgtcgg
     7021 ataccaccga actggttgat ttagccgtga tcttccctga tctggagatc gaattgaaat
     7081 atgcctgcgt tgataacatc acaggtaaag ctatttatca gcaagcgcgt tgtctgctac
     7141 ataaagatgc gattaccgcg ctggcgaaaa gtatcagtat cgcccagctg tcaggcttac
     7201 aactggtgat ttacgatgcg tatcgcccac aacaagcaca ggcgatgttg tggcaagcct
     7261 gcccagaccc gcaatatgtt gttgatgtga cggtcggttc taatcacagc cgtggcacgg
     7321 cgctcgacct gacgcttcgt gatgacgtgg aggtggtgaa tgatgctaag cgaggaaacg
     7381 tccgccgtac gcccacaaaa acaaacgcga tttaacggtg caaaactggt atggatgctg
     7441 aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg tattaatgcg actgatgatg
     7501 attttttcac cgtggctggc gacgcatgat cccaacgcca ttgatttaac cgcccgcctt
     7561 ttgccgcctt ctgcggcgca ctggtttggc accgatgaag tgggacgcga tctgtttagc
     7621 cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat tagtggtggt cgccattgcg
     7681 ggtatgattg gttcgctact cggatgtcta tccggtgtgc ttggcggacg cgcagacgcc
     7741 atcatcatgc gcatcatgga cattatgctg tcgattcctt cgttggtact gacaatggca
     7801 ctggctgccg ctctcgggcc gagtttattt aacgccatgc tggcgattgc tattgtgcga
     7861 attccctttt atgtgcgctt ggcgcggggg caagcattag ttgtacgcca gtatacctat
     7921 gttcaggcgg cgaaaacctt tggcgcgtct cgttggcatc tgatcaactg gcatatttta
     7981 cgtaactccc taccgccgct gatcgtgcag gcatcgctgg atatcggtag cgcgatttta
     8041 atggccgcca cgttgggatt tattggcctg ggtgctcaac aaccgagtgc tgaatggggc
     8101 gcgatggtag cgaatggtcg caactatgta ctcgatcagt ggtggtattg cgcatttccg
     8161 ggggcagcga ttttgcttac ctccgtcggg tttaatctct ttggtgatgg tattcgcgat
     8221 ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca acccgttctg gacattcaac
     8281 agctgcattt gagtttcccc ggttttaacg gtgatgttca cgcgctcaac aatgtgtcct
     8341 tgcagattaa ccgcggcgaa attgtcggtc tggtgggaga atccggctca ggtaaatcag
     8401 tcaccgcaat gctgattatg cgtctgctac cgacgggcag ttattgcgta catcgggggc
     8461 agatttcact gctgggagaa gatgttctta acgctcggga aaaacagctt cgtcagtggc
     8521 gcggcgcacg agtggcgatg atctttcagg aaccgatgac cgcccttaat ccgacacgtc
     8581 gaataggtct tcagatgatg gacgtaatcc gccatcatca accaataagt cgtcgggaag
     8641 ccagagctaa agcgattgca ctgctggaag agatgcaaat cccggatgcc gtggaagtta
     8701 tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca gcgggtaatg attgcgccgg
     8761 cattctcctg cgagccgcaa ttgattattg ccggcgaacc gacgacggcg ctggacgtca
     8821 cagtacagtt gcaggtactg cgtttgctta aacataaagc acgcgccagt ggaactgcgg
     8881 tactgttcat cagccatgat atggccgtgg tgtcgcaact gtgcgatagc gtttacgtga
     8941 tgtatgccgg aagcgtaatt gaaagcggcg ggacggcaga cgttatccat catccccggc
     9001 atccgtatac cattggtttg ctgcaatgcg caccagaaca tggagtacca cgccagccat
     9061 tacccgccat tccagggacg gtaccaaacc tcacccattt gcctgacggc tgcgcttttc
     9121 gcgatcgttg ctatgcggca ggtgcacagt gtgcgctgac agcgtgtggt gacaacaacc
     9181 agcactgcac ctgttggtat ccgcagcagg aggtcataag tgtctgacac gttattaacg
     9241 ttacgcgacg tccatatcaa ttttccggcc cgtaaaaact ggcttggcaa atctacggaa
     9301 catgttcatg ccattaatgg tattgattta cagatccgcc gtggtgaaac cttagggatc
     9361 gtcggcgagt caggctgcgg caaaagcacc ctcgcacagc ttttaatggg catgctgcaa
     9421 ccgagccacg ggcagtacat ccgttcaggc tcacaacgca ttatgcagat ggtgtttcag
     9481 gacccgctct cttcgcttaa tccgcgctta ccggtgtggc gcatcatcac agaatcgctc
     9541 tggatagcta agcgtagtag tgaacaacag cggcgagcgt tggcagagga gttggctgtg
     9601 caggtgggta ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc cggcgggcag
     9661 cggcaacgca tcgccattgc cagagcactc tcttcgcagc ctgacgtgat tgtgcttgat
     9721 gagccaacct cggcgctgga tatctccgtg caggcgcaga tcctcaattt gctggtaacg
     9781 ctacaggaaa atcacgggct gacttatgtg ctgatttcac acaatgtctc ggtgatacgt
     9841 catatgagcg atcgggtggc ggtgatgtat ctcgggcaga ttgtagaact gggcgacgcg
     9901 caacaggtgt tgactgcttc ggcacatcca tacacccgat tattgctgga ttccctcccc
     9961 gccattgata aaccgctgga ggaagaatga gcattacgta aaacggatct gccgggaaac
    10021 cgcacgttgc cgcaaggctg ttttttctac gaacgttgcc ctctggcaac ccacggatgt
    10081 gaagtccggc aatcattagc aataagggag gacggacgtg acctccggtg ctggcgggcg
    10141 ctgtagagat gctaacaacg catcaggcat tcacgcgcct gacgcgtcat ccggcaatgc
    10201 tttacgattt caactggtaa tccagcgtaa tttccgcttt cagtacctga gagaccgggc
    10261 atcctgcttt tgctttctgg atgatgccgt caaaggtaga ggcatcaata cccggcaccg
    10321 caacttcact cttcagtgcg attttcgtaa tcgcaaaacc ggcatccact ttatccagcg
    10381 acacatcggc ggtggtatca attgatgttg gcgtgaatcc cgcttccccc agcattaatg
    10441 aaagcgccat tgagaaacat gcggcatgcg ctgcgccaat cagttcttca gggttggttc
    10501 ctttttcgcc ttcaaaacgc gtgttaaatc catacggctg ttggttcagc acgccgctct
    10561 cggtggatac tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc tgacctttct
    10621 tatggattgt cattgttgct ctcctgtggg cttaaaaagg aaattataga ctacgtgaga
    10681 aacagcacga ggataagcag gatattccga ataaaatacg gcgaaaatat tacttctgca
    10741 cttaaattaa attcccacaa tagataaccg ggaatatata tctcaaga
//
LOCUS       DAZPDE010000128        10712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPDE010000128 DAZPDE010000000
VERSION     DAZPDE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.128"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            74..904
                     /locus_tag="ACHU0J_002996"
     CDS             74..904
                     /locus_tag="ACHU0J_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1217587.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1045..1818)
                     /gene="uxuR"
                     /locus_tag="ACHU0J_002997"
     CDS             complement(1045..1818)
                     /gene="uxuR"
                     /locus_tag="ACHU0J_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1217588.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2033..3493)
                     /locus_tag="ACHU0J_002998"
     CDS             complement(2033..3493)
                     /locus_tag="ACHU0J_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1217589.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3574..4758)
                     /gene="uxuA"
                     /locus_tag="ACHU0J_002999"
     CDS             complement(3574..4758)
                     /gene="uxuA"
                     /locus_tag="ACHU0J_002999"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1217590.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5098..6441
                     /gene="gntP"
                     /locus_tag="ACHU0J_003000"
     CDS             5098..6441
                     /gene="gntP"
                     /locus_tag="ACHU0J_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1217591.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6692..7594)
                     /gene="fimH"
                     /locus_tag="ACHU0J_003001"
     CDS             complement(6692..7594)
                     /gene="fimH"
                     /locus_tag="ACHU0J_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1217592.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7614..8057)
                     /locus_tag="ACHU0J_003002"
     CDS             complement(7614..8057)
                     /locus_tag="ACHU0J_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1217593.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8130..8660)
                     /gene="fimF"
                     /locus_tag="ACHU0J_003003"
     CDS             complement(8130..8660)
                     /gene="fimF"
                     /locus_tag="ACHU0J_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1217594.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8670..>10712)
                     /gene="fimD"
                     /locus_tag="ACHU0J_003004"
     CDS             complement(8670..>10712)
                     /gene="fimD"
                     /locus_tag="ACHU0J_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1217595.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2563 a         2776 c         2640 g         2733 t
ORIGIN      
        1 cttctctttt tactacagaa aaatttatct atttttctaa acatcagtta aatactcata
       61 aaaggtaact attatgtcta tgccattaag caatgccgct aataaccaat tcaatacaaa
      121 taatcacttc cttcatcatc caaaagtcga cagtgagtta acacgtcgat atgaatatgc
      181 acgcctggat acggaaaata tctatcttct gcctcttgct cgtggcaata accataatta
      241 cgatggaaaa agcgttgtcg aaattagaaa acttaatatt ggcaaacagt cctggccatt
      301 taattatgtg acagatacat gcagagaatt caatggtatt acaacaacag gacgtatgct
      361 ctatcgaaac ttgaaaatca cctcagcttt agacgaaata tatggtggta tatgtaaaaa
      421 ggcgcatgct gctacagagt tagcagaagg tttacggcta aatttattta tgaagagccc
      481 tttcgatcct gttgaggact atacagttca tgaaataaca ttaggccctg gttgcaatgt
      541 tcctggttat gccggaacca cgataggata tatttcaacc cttccagctt ctcaggcaaa
      601 aagatggacc aatgagcagc caagaattga tatctatatt gatcagataa taactgtctc
      661 aggggtagca aattcttccg gattcgccct tgccgcatta cttaatgcta atattgagat
      721 gggaaatgac ccaatcattg gtatagaagc ctatcctggt accgcggaga tccattcaaa
      781 aatgggatat aacgttatcc ccggtgatga agatgcgcca ctcaaacgca tgactctgca
      841 accgtcatct cttccagaac tgtttgaatt aaaaaatggt gaatggaatt atattggtaa
      901 atagccactg agaagaatga gtactaatat tcaggttata cagatattta aacaaaaccc
      961 tgcaatttac aaagtaatac tcattgcgac ttcttaattt aagaaagcgc aaggaacgtt
     1021 tacccttgcg cttattataa taagtcaggc gtcgacttta tccagcggcc atgaatcaaa
     1081 gagatagccg tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgcttaac
     1141 gttttccaga tgctgccaca ttgccagctt cgccgctcgg gcatcttttt tgattaacgc
     1201 ggcgaggatc tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catccagatg
     1261 gctgtgcaac tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaataa
     1321 ctccaccagc atgctgttat gcgttgcttc agcaatggcg agatggaact gcatgtcacc
     1381 gctttcgctg ctacccggcg cgctggaagc cagttcacgc tcttccagtt gcaatgcctg
     1441 acgcattttg acgatatctt cgcgggtagc ctgcagtgcg gcaaactcgg cgatgttact
     1501 ttccagtaac tgacgcgcct gtaacagctc aaaaggaccc gcatcattgc agacgttggc
     1561 atccggactg tctgtgttgt ggctgcctga gctatcaagc acatagatac cggcaccccg
     1621 gcgcacttcc accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg
     1681 cgtgacatca agcatttctg caatttcacg ctccggcggc agccgttcgc caggattgta
     1741 cggcgtcttt atgatcagat cgtggatcat cgccccgact tcctggtaag gtctttgcgc
     1801 agaggtgaca gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa tttttgccgg
     1861 atgcggcgtg aacgccttat ccggcctaca acgggcaatt ttacggcccc cgttaatctc
     1921 aaactatacc attagcactg gaagttggca cgtacaggtg ggcaaaaaaa cgaaaactgg
     1981 tcaaccaaat atcagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg
     2041 cagccacaca ctcgcgcgca ccgcgttcgc acagctgctg atatgccgac gtcaccgcgc
     2101 caacaaagtc ggcattctgc ggcagatcat cggcaaaaat accgctcagg cccagcagcg
     2161 ctttcacgcg gtctgcgccc tgatactgcg cgttaatctt ctggaactcg gccagcatcg
     2221 ggtcgaccac gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cccatccagc
     2281 cagccacgcc cagcgccagg tgacgccagc tgccgccgtt ttgcaggtgc agacgcaccg
     2341 ggtccagtag acgctgcggt aacttctggc tgccgtccat cgcaatctgc caggtacggt
     2401 gacgcagaga cgggttgctg aaacgctcga tcagcagcgt cgcataggcg ttcaggtccg
     2461 taccttccgg catcgacagc gttggcgctt gttcctgcat catcagggca aaggccgctt
     2521 tgcgataatc cgggttagtc atggtgtcgg caatggtttc atagccgccg aggtaaccga
     2581 ggtacgccag gaaagagtgg ctgccgttca gcatacgcag cttcatcatt tcgaacggca
     2641 caacgtctgc aacgaactgt gtgcccactt tatcccagtc cgggcgacca ttaacgaagt
     2701 tatcttcaat cacccactga cggaacggtt cgcaggcaat ggcgcacggg tcgtaaacac
     2761 ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc cgccggaacg atgcggtcaa
     2821 ccatggtgca cgggaaggtg acgttctctt caatccatgc cgctagctgc gggtcacgcg
     2881 cctgagccag ccccagtacc gcgaccttcg ccacatggcc gttttcacgc acgttatcgc
     2941 aggacatcac cgtaaatgct ttcagccctt tttcacgacg cagacgcaag gcttcgacga
     3001 tgtaaccaat cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg atcagcgggt
     3061 tattgagatc cagttgaccg cttgccgcat cagcgcagta gcctttttcc gtgaccgtta
     3121 gagagacaat cgccgtttgc ggacgcgcca tcgcgttgag aataccttcg cagccgtcga
     3181 tttccggatg cagcgcttct ttcatcgaac cgataatttt cagctcggtg ctttctgcgc
     3241 ctttttccgc tacggtgtac agcagttgct gttttttcag gttttcgatc agcatgcggt
     3301 cgttgcctgg catcaggtta acttcgcaga tgccccagtc gctgtcggtg ctttccagca
     3361 gatgatgggt atacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg agatgcacaa
     3421 tgcgtgattc cagacgagaa tgatcccatg acgggcgggc aaccggcaga ttgctgtcaa
     3481 caatagtagt catcgtaaac tccagaatcc gtcttcagag agtgacggca aggaagaagg
     3541 aaccggcacg gcgcgcagcc atgccggtgg gtattaacgg ataaagaaag cgcgctggat
     3601 cgccagttcg acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc
     3661 agggttggtt ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg
     3721 gatcaggtct tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac
     3781 cactttgtac atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt
     3841 atcttcacgc atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat
     3901 catatcaacc agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtgaaacc
     3961 gttcgccatg ctgtttacgg tatcaaccat ccactgcata tcttcaatgg tggaaacaat
     4021 gcgcggcaag ccgaggatcg ggcgcggcgg atcgtccggg tgaaccgcca tacgcacgcc
     4081 aacttcttca gcaactggaa taatcgcttt caggaagacg gcaaagtttt cgcgcagttt
     4141 ggctttgtcg atatctttgt acagctccag gtgcttacgg aactggtcga gggtataccc
     4201 ttcttccgcg cccggcagac cggcaatgat gttacgcgtc agacgcgctt tgtcttcgtc
     4261 gctcatagtg gcgaagcgtt cagctgcctg agcaatttct tcttcggtgt aatccgcttc
     4321 cgcgcctgga cgtttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg
     4381 cagagctttg gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac
     4441 cggcatgaag ttgtagcaca cggtgcgaat gccacactgc gccaggttgc gcagagtctg
     4501 ctgatagtta gcgatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat
     4561 tggtacgctt tctacgacag accacaccag gcctgcgtct tcaacgatcg ccttgcgttt
     4621 gaggatctct tctacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac
     4681 gccagttgcg cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca
     4741 gcgccaggtc tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac
     4801 gcggaacctt ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg
     4861 catcggatga gatggcgtat aagttctacc ttttggtaag acatgtcaac cagaattctg
     4921 aaattggtta accgcgtcgc aagaatttca ctctcatggt tgttgctgca aattgcagca
     4981 aagggtaatc gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca
     5041 atttacataa catgtcgccc aaaataacaa cggttcaacc aagtaaggta aaacgttatg
     5101 catgtgctta acattctctg ggtggtattc ggcattggcc tgatgctgat actgaatttg
     5161 aagttcaaaa tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt cgggatgctg
     5221 gcaggcatgg atttgatgtc gctgctgcac accatgaaag cgggcttcgg caacacgctg
     5281 ggagaactgg ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat ggtcgactcc
     5341 ggcgcggctc accagatagc acatacgctg ctggcgcgtc tcggtctgcg ctatgtacag
     5401 ctgtcggtga ttatcatcgg ccttattttc ggtctggcga tgttctatga agtggccttt
     5461 atcatgttag cgccgctggt tattgttatt gccgccgaag ctaaaattcc gttcctgaaa
     5521 ctggcgatcc cggcagtagc agctgccact accgcacatt cactgttccc accgcagccg
     5581 ggtccggtgg cgctggtgaa tgcttatggc gcggatatgg ggatggttta tatctatggc
     5641 gtactggtga cgatcccaag tgtaatctgc gcaggtctga tcctgccgaa gttcctcggc
     5701 aatcttgagc gcccaacgcc atcattcctg aaagcagatc aaccggtaga tatgaataat
     5761 ctgccctctt tcggcgtttc gattctggtg ccgctgatcc cggcgatcat tatgatctct
     5821 accaccatcg ccaatatctg gctggtaaaa gatacccctg cctgggaagt ggttaacttt
     5881 attggttcct cgccgattgc gatgtttatt gcgatggtgg ttgcattcgt actctttggc
     5941 accgcgcgtg gtcatgacat gcagtgggtg atgaacgctt ttgaaagcgc ggtgaagagt
     6001 attgcaatgg tgattctgat catcggtgcg ggtggcgtgc tgaagcagac catcatcgac
     6061 accggcattg gcgacaccat cggcatgttg atgtcccacg gcaatatctc gccctacatt
     6121 atggcatggc tgatcactgt gctaattcgt ctggcgacgg gtcagggtgt cgtttcggcg
     6181 atgaccgccg ccgggattat cagtgctgca atccttgatc cagcaaccgg tcagctggtt
     6241 ggcgtgaatc cggcgctgct ggtactggcg acggctgcgg gttccaacac cctcacccac
     6301 attaatgatg catctttctg gttgttcaaa ggttactttg acctgtcagt aaaagacacg
     6361 ttgaaaacct ggggtctgct ggagctggtc aactccgtgg ttgggctgat tattgtgctg
     6421 attattagca tggtagcgta aaagaacacc agagcctgcc aatggcaggc tcagactgat
     6481 gactaaagtc taaaaatgtg cccggacagt cccctcgccc ctccggggag agggttaggg
     6541 tgaggggaac agaccagtaa agtgcgcttt gtttatgcca gatgcgacgc tgacgcgtct
     6601 tatctggcct acaaagggct aacgtgcagg ttttgtaggt ctgatagcgt agcgcctcag
     6661 gtaccagcat tagcaatgcg ctgtaatttt tttattgata aacaaaagtc acgccaataa
     6721 tcgattgcac attccctgca gtcacctgcc ctccggtacg tgcgtaattt gccgttaatc
     6781 ccagacttac cgccgaagtt cctactgctc ctaacgatac cgtgttattc gctggaataa
     6841 tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc tggtgaaaac gacgcggtat
     6901 tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga gaggtaatac cccaggtttt
     6961 ggcttttcgc acaataaacg gtaagaggaa ttggcactga accagggtag tccggcagag
     7021 taacggtgac atcacgagca gaaacatcac aaccgccagt gggcaccacc acatcattat
     7081 tggcgtaaat attccacaca aactgaaaat catcgctgtt atagttgttg gtctgtcgca
     7141 aaataagcac ggcaattaat gaaccagctt taatagccac cccgcccgca ctgctcacag
     7201 gcgtcaaata aagcgccacc ggccacggct tatccgttct cgaattataa acaacccgcg
     7261 gcgtttcgct ggtggtcgga aatggatagc tactgccact atattttacg gtcccggaaa
     7321 aattagataa cacgccgccg taagccgagc ctcgttgcag tgtgacatag tctgtaatgg
     7381 tttccggata atcgttatgg caaaagattt gcgtcgaaag atctacgacc aggttttgcc
     7441 ccacattcac gacaggcgca aggtttacat aaacattagc gctgccaccg ccaataggga
     7501 tagcggtacc attggcggtt ttacaggcga atgaccaggc atttaccgac cagcccatca
     7561 gcagtacagc aaacagggta atagctcgtt tcattacaat tatctcttcg ggttcagctg
     7621 taggtatagg tgatgctaat cactgcctga atggttccct gagtggctcc gccatttacc
     7681 gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg aggaatcatc cacctgaact
     7741 gttttggttg cgccagtatt caatgtgttg ccactgtcat cctgtagctc taactggatg
     7801 ttttgcgcgg tcccctggtt tttataatat ccggtgctgt cggctgcccc gctgaagctg
     7861 gcagtgaccc tcgacgttcc caccggacaa ttagtcaact caagcgcaac atcatgccag
     7921 gccgatgccg ccccggcaga cataagactg aaagaataaa gatcgccgag atctaccgtg
     7981 gcattggtgg tggaaaccgt acacggtttg gcgacgacct taccgttcac cgtgatggtg
     8041 acatcggctg cctgtatcgt cgcacttgcg agcgccaaca ttgccgccaa tacataccca
     8101 cgtttgcacc atttcatgag catctccagt tactgatatt caagagtgaa ggtagccgta
     8161 gcattgatat gccccgcagt gacaggcacc tgtgtcgcca ttagccgggc gtaaaaattc
     8221 agcgtatttg gtttacccgg cgtcagggtc gtccacgaaa tcgcagacga tggagcatta
     8281 aggggtattt gattttgctg ctcattcaga agctgtattc ccagtcccgc agccgctgac
     8341 accgtatttt caagtgcaag caggttggca ttgtggctat ctgcaacgcc ggtaaaccca
     8401 acttttacgg cagaaacggc attaccacag ggtgacagca aaatacgaaa tggaacgaca
     8461 ggagtcgtcg cgccaatgtt gttaaattgc ttcgccgcgt tttccatcag atcaacagta
     8521 aaattggttg attcagcggc cacactacag ccgttatctc tgacatagcc gcggatagta
     8581 atcgtgctat ccgcagccaa aacgcgactt accgccagcc acaaaaaagc gcacagaaga
     8641 taaaaaggtt tgtttctcat cacgccccct taacgacatt cagctgatag ctgggttaat
     8701 aactgctgct gactctctgg tggcagttga taattggcga cacagtgagc attttcctct
     8761 tctccccatt tcacctgaac ttttcccgct aaaggcattc cgctgaggta aacctgacca
     8821 ttatccgcaa caatgccgct actctggcta ctctctgatg tcaccatcgc cccaaacggc
     8881 agcggcttat tattgtgagt tagcgtcatg agcagtttta tcccaacgcg cgctttaaac
     8941 tctgctcgca cgatcgcccc acgagtggga acaacgttag cgaccgcgtt atctaaatcg
     9001 acgttatcag ccaaggtatt ggtatccagc gccactctat tttcccgata ttcagtggca
     9061 taaggcagca cggcataacc acgccagtcg gtacgcaccc ccgtctggtt ttcgactttt
     9121 gcatcttttg cgccaggcgc tttaacaagc accaccgtat cgtttaacgg ctgccccagc
     9181 gttacgccat tggcatgagc caatacccca ccgctgactc cgtaatagag ctgcttaata
     9241 tcatcgctat ggctgtaacc gatattggca ttgccgtaac caccgcgata attcagcgtg
     9301 gcgtagcctg tacttccgct attaccatcg cctcccccgg catagccggt ttgcacgcta
     9361 taactgaggt tgttgtcttc cagcaacgta ccgtatacac cagccagatt ggtcatccga
     9421 ccgttgagat cgtgtgacat gctgtagctg gcactggcat gtcgccactg agatttactg
     9481 tcagaacgca gccagtggct gaaaggaata ttgacgttaa gcgctaacat ctgatcccgt
     9541 cctttttgcc aggcgttttt cgtcaggcta tagctgagcg tccagttgat atcttcgaac
     9601 gcagtattta atccagcctg gaattgctca tcgacattac tcgttcccca ataagtttga
     9661 tggctaccac tcaaatacag tgttgatgtg cgcccgagtt gctgagtaac ggtgagctgt
     9721 aatttcccgc gtttgttata agcgaggttg taatagtcgg tgaatttcgg cttaacctga
     9781 ataactccat cctgtgtttc gatgttgtag ccattcattc gactgtatgt tgtatcagcg
     9841 aaattaaaat atccgctggt cgaataacgg taacccacta actgaatatt cgtgcctgat
     9901 tcattgagcg atttgttata gagaaaacgc accgattgtc cgtcatgctg actgtcatcg
     9961 ggaagtgtgg aattagcctg cgtcatatcc acagacagag cgcccagtgc ctccatgttt
    10021 ttcccgatac cgaaattaaa agcacgataa cgatccgcca gttgcgttcc accatatatt
    10081 gtccagccag ccggaaggcc gtggagtaat gtactctgga aaaagcgggg tttttcctgc
    10141 tgcgcatttc cactacggta ttctcctgcc gtaatggaat aacgagtatg cccttcacgt
    10201 tgcaaaagcg ggactgacga atagggtacg gtaaaaatct gcgtgctgcc gtcagcctct
    10261 ttgatcgtta cctgcaagtc accactatta cctgcggcat agatatcgtt gatggtaaaa
    10321 ggccccggtg gcaccgtact attataaatg ccatatccat tttgtttaat agtgacctgt
    10381 gcagtaccac gagcaatacc gtggatcacc ggggcaaatc ctctttggct atcgggtaac
    10441 atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa taccatcgaa aatatcgccc
    10501 tgggtataac catcacccaa cgtcagccgg gaacgtaaag gaattatgtc tcgctcaatc
    10561 caggtaatga tatgctgcca tttatttttg ctacctgatg aactgtcgct actgttataa
    10621 ctccaggtgg tattgtcgcg taaacgccac gcaccaatat ttaacccact ctgtaggttt
    10681 aaatatgcat aatggctgtt acccccaatc cg
//
LOCUS       DAZPDE010000129        10678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPDE010000129 DAZPDE010000000
VERSION     DAZPDE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.129"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1817)
                     /gene="selB"
                     /locus_tag="ACHU0J_003005"
     CDS             complement(<1..1817)
                     /gene="selB"
                     /locus_tag="ACHU0J_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1217596.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1814..3205)
                     /gene="selA"
                     /locus_tag="ACHU0J_003006"
     CDS             complement(1814..3205)
                     /gene="selA"
                     /locus_tag="ACHU0J_003006"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1217597.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3303..3911)
                     /locus_tag="ACHU0J_003007"
     CDS             complement(3303..3911)
                     /locus_tag="ACHU0J_003007"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1217598.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3982..5067)
                     /locus_tag="ACHU0J_003008"
     CDS             complement(3982..5067)
                     /locus_tag="ACHU0J_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1217599.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5070..5432)
                     /locus_tag="ACHU0J_003009"
     CDS             complement(5070..5432)
                     /locus_tag="ACHU0J_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1217600.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5969..7882
                     /gene="mtlA"
                     /locus_tag="ACHU0J_003010"
     CDS             5969..7882
                     /gene="mtlA"
                     /locus_tag="ACHU0J_003010"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1217601.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7977..9125
                     /gene="mtlD"
                     /locus_tag="ACHU0J_003011"
     CDS             7977..9125
                     /gene="mtlD"
                     /locus_tag="ACHU0J_003011"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1217602.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9125..9712
                     /gene="mtlR"
                     /locus_tag="ACHU0J_003012"
     CDS             9125..9712
                     /gene="mtlR"
                     /locus_tag="ACHU0J_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1217603.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9724..9933)
                     /gene="yibT"
                     /locus_tag="ACHU0J_003013"
     CDS             complement(9724..9933)
                     /gene="yibT"
                     /locus_tag="ACHU0J_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1217604.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10217..10579
                     /locus_tag="ACHU0J_003014"
     CDS             10217..10579
                     /locus_tag="ACHU0J_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1217605.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2568 a         2885 c         2752 g         2473 t
ORIGIN      
        1 cgcaataaat gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga
       61 atctgaattg ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt
      121 gaaccacact cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta
      181 cggtaataac gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc
      241 gtcaggcgca ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac
      301 ggctcgtcac caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag
      361 cccgctttgt gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg
      421 cgcatctttt caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt
      481 cgcagacgtt cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat
      541 gtgtcgagaa ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca
      601 gcctgaatat agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc
      661 gcccaagcga aatcaggaag attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg
      721 ctatcattct gtgccctggc gagggaagcc agccattgca gatactctgg tttacgttta
      781 ccgcgacgcg gcgggttaag catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag
      841 atatcgcgca ataccaggcg gtcgttatct gccagccata acggggtgtc gaagaccagt
      901 tcagcaaggt tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga
      961 atatgcagcg gctgccactg ggtcagcggt gcatgggttt gaagctcgac aatcacccgt
     1021 gtgaacggct ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt
     1081 tccgcatcac ccgcgatgtc aagcgcgata cgctgcccgg cattggcggt ttctgttggc
     1141 tggttttgcg catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt
     1201 gaatcgccta ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct
     1261 tttacggtaa atgcgcggtc aatcgcgagg cggaaactat gttggctggc gtgctcgcgt
     1321 tccggcaact gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg
     1381 gtgataaaca gttttgcctc agcaaaaccg tattcccgca gaacctcctt tacctggcgt
     1441 tcaacctcat caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc
     1501 atcgggttac cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg
     1561 tcatcgcacg ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg
     1621 gaaagaaact tttcatgacc gggaacgtcg ataaaaccag gcacgcgacc atccggctgc
     1681 ggccagtagg catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg
     1741 tcagcattta cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg
     1801 gcagtcgcaa taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc
     1861 gtaaatccag ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac
     1921 gccagcgggc ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta
     1981 atgccgcgct cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg
     2041 gcataacctg taccgcaaac tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt
     2101 gtgcctggat ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt
     2161 cactcagagc ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca
     2221 ttttatccgc gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt
     2281 ttttaccaac gataatcccg gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca
     2341 ccagactgac gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact
     2401 ggctaagatc gaccagcgag ccactgccta aatcagtcac cactggaaca tccagctctt
     2461 tgccgagcgc caccagttca gcttcgtcta tcgctttggt gaacccctga atgctgtagt
     2521 tactggtatg tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat
     2581 tcgcgtgcgt gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa
     2641 catcgggaat acgaaacgcg ccgccaatct ccaccagttc gccgcgagat accaccacct
     2701 ctttcccgct ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattgacga
     2761 tacaggcatc ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat
     2821 gtccgcgtcc ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg
     2881 caacggcttc caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg
     2941 ttcccgtcag gttgatcacc ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat
     3001 cgacttcttg cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt
     3061 ctcgcgcttc gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat
     3121 cacgcaaaga aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat
     3181 agagggaacg cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga
     3241 gaagggcgtt taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg
     3301 catcaagcct ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc
     3361 agtttgacta agtgtgggcg gtcgacacac caacctggcg caacgcggcg gaaattgaga
     3421 tatccgacag cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg
     3481 ccatcgacca gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt
     3541 aacaattcat cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc
     3601 ccggcatcca taatgccatc cgccagcgcc tcaattttgc gcacccgcaa cgactccagc
     3661 ggatcgcgcg gcaacatcgc cggagcgaca ttcattaact caatatattc agcgatgatc
     3721 ggcgaatcaa accagcattc gccctcttcg gtcaccagca ccggcacttt ccctaacggg
     3781 ttaaattgcg ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaaagtt
     3841 atgccctttt ctaacaacag aataaaaagt ttgcgtacaa acgggctggt gtagctaccg
     3901 acgagtttca tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcacgc
     3961 aaaacacagg tgatataagt ctcaatgatc caaataaaga taatgcatcc aactggtcat
     4021 tcttagcagc actttccgca tcaccgaaac atggctgaaa tggtcggagt aaaccgccac
     4081 ctgggcgtag atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt
     4141 tcccagcaca ccgtccgtgc cgggaacgac cgttaatgat tgcaataccc cctgcgcctg
     4201 ataagaaccg cctggcacga caggtaaaat actagtcaat ttgccgtgga acacctgccc
     4261 aggtagcgcg ttaaacacca cttccgcatc atcgccaggt ttcagacgta acagcgagtt
     4321 ttgccgaaat tgggcgacaa tttgccgttt ttgctcgggg atgaagacca tcaccggacg
     4381 cagcggcaag gcagctgcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt
     4441 gcttggtgcg cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct
     4501 taagctcaca atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg
     4561 cgcctgctcc gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc
     4621 atcgtcgatg tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg
     4681 ttgataattt ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc
     4741 ttcagtgagc tgcgcacgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg
     4801 aagtctgtca actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt
     4861 ttgaataagc tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt
     4921 gatagggatc gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat
     4981 caacaaaatc aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgccac
     5041 gtaagttaaa acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc
     5101 tttttctcgg cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt
     5161 tccggttcag tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc
     5221 ggttgataga gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg
     5281 ctcacccagc ccgccgtatg aatagcgtcg gcatgggggt gattgcgctt tttggcaatc
     5341 agataaggga tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc
     5401 agcactccca acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg
     5461 ttgttttttg gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt
     5521 cgcgattctc gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca
     5581 taacgattgt atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga
     5641 aaatatcttg tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat
     5701 tactcacatt taaatgccat ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg
     5761 cgttttgtga tgagcgtcac gtcaattacc tctctacccc ctatatttat gtgactgata
     5821 tcacacaaaa gcccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct
     5881 acaaggtccg gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc
     5941 gacagcaaac ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag
     6001 ctttggtcgt ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg
     6061 tatcatcacc gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct
     6121 ggtcgggccg atgatcactt atctcctacc gctgctgatc ggttataccg gtggtaagct
     6181 ggtaggcggc aaacgtggtg gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg
     6241 cgcagacatg ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat
     6301 taagcacttc gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa
     6361 taacttctcc gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg
     6421 cccgattgtt gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca
     6481 tgacatgctg ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa
     6541 cgccattaac cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa
     6601 atcaatcttc ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta
     6661 catgttcttt ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt
     6721 cctgggtggt atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct
     6781 cgcagtcatc ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct
     6841 ggtttctccg gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc
     6901 ttatttcgct aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc
     6961 tattttgctg aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg
     7021 tatgcaggac atgaaagctg agtctaaagg cacatctccg ctatctgcgg gcgatgtgac
     7081 taacgacctg agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag
     7141 tgcgatgggc gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc
     7201 ggtcactaac agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg
     7261 tgacctgacc gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa
     7321 cttcctcgac agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca
     7381 tactgaaaac gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc
     7441 taacctgttc aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga
     7501 agaagcgatt cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata
     7561 cgttcaggcg atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc
     7621 ggtgccacac ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg
     7681 ccagtacccg gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat
     7741 tggtattgca gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact
     7801 ggatgatgag tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga
     7861 actgctggca ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg
     7921 agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga
     7981 aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag
     8041 acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg
     8101 cccgtcatag ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg
     8161 gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt
     8221 tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag
     8281 ggctggtgaa acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa
     8341 acatggtacg cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg
     8401 ccaaagcgtg ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac
     8461 cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat
     8521 ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa
     8581 ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta
     8641 taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg
     8701 agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc
     8761 gctacggctt tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg
     8821 agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga
     8881 gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca
     8941 agaatctgat tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccacagg
     9001 ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt
     9061 ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc
     9121 aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca
     9181 ggcaacaatg gaacaaaccc aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg
     9241 caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct
     9301 tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt
     9361 actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt
     9421 gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga
     9481 gctaaatcac gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg
     9541 tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag
     9601 tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc
     9661 actgactgag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc
     9721 tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg
     9781 tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag
     9841 ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat
     9901 aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc
     9961 gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac
    10021 gtgctgaatc ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa
    10081 ttaatcaacg aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca
    10141 atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt
    10201 tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg
    10261 cctggacgcc atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata
    10321 tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt
    10381 tcatagccaa aaactgagta aagaagcaca gaagctgatg aagatgccgt tccagcgcgc
    10441 aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt
    10501 ggttgtgcac ccaatgaccg cgctggggcg cgaaatgggc ctggaagaga tgactggatt
    10561 ttctaagact acgttttaag cccctcagac gactggttcg atcgtatcaa cccgcatctg
    10621 gcttgcctta gctggtcaat gcggggactc tcctgtctac tcgcctaatt accaaaca
//
LOCUS       DAZPDE010000130        10464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPDE010000130 DAZPDE010000000
VERSION     DAZPDE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.130"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACHU0J_003015"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACHU0J_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1217606.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACHU0J_003016"
     CDS             213..401
                     /locus_tag="ACHU0J_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1217607.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACHU0J_003017"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACHU0J_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1217608.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACHU0J_003018"
     CDS             596..769
                     /locus_tag="ACHU0J_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1217609.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACHU0J_003019"
     CDS             complement(818..1891)
                     /locus_tag="ACHU0J_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR1217610.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHU0J_003020"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHU0J_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACHU0J_003021"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACHU0J_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1217611.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHU0J_003022"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHU0J_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1217612.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACHU0J_003023"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACHU0J_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1217613.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="ACHU0J_003024"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="ACHU0J_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10464
                     /gene="torD"
                     /locus_tag="ACHU0J_003025"
     CDS             10276..>10464
                     /gene="torD"
                     /locus_tag="ACHU0J_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1217614.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagttcatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cacg
//
LOCUS       DAZPDE010000131        10454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPDE010000131 DAZPDE010000000
VERSION     DAZPDE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.131"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..2036
                     /locus_tag="ACHU0J_003026"
     CDS             <1..2036
                     /locus_tag="ACHU0J_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1217615.1"
                     /translation="LKNDYKISLSPDTTYRELNKAAQSSITAPDRIGEGKTWVYQRDT
                     MVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPS
                     GQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIREN
                     MSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFI
                     ALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQ
                     VLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKNLHALKEKSLNGMPKT
                     TPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTN
                     LFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGD
                     LLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALP
                     FSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGI
                     WSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNM
                     GSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGKTGEPPNYLYL"
     gene            2372..3304
                     /gene="glaH"
                     /locus_tag="ACHU0J_003027"
     CDS             2372..3304
                     /gene="glaH"
                     /locus_tag="ACHU0J_003027"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1217616.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3324..4592
                     /gene="lhgO"
                     /locus_tag="ACHU0J_003028"
     CDS             3324..4592
                     /gene="lhgO"
                     /locus_tag="ACHU0J_003028"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1217617.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4615..6063
                     /gene="gabD"
                     /locus_tag="ACHU0J_003029"
                     /pseudo
     CDS             4615..6063
                     /gene="gabD"
                     /locus_tag="ACHU0J_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6071..7357
                     /gene="gabT"
                     /locus_tag="ACHU0J_003030"
                     /pseudo
     CDS             6071..7357
                     /gene="gabT"
                     /locus_tag="ACHU0J_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7595..8995
                     /gene="gabP"
                     /locus_tag="ACHU0J_003031"
     CDS             7595..8995
                     /gene="gabP"
                     /locus_tag="ACHU0J_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1217618.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9016..9678
                     /gene="csiR"
                     /locus_tag="ACHU0J_003032"
     CDS             9016..9678
                     /gene="csiR"
                     /locus_tag="ACHU0J_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1217619.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9679..10128)
                     /gene="kbp"
                     /locus_tag="ACHU0J_003033"
     CDS             complement(9679..10128)
                     /gene="kbp"
                     /locus_tag="ACHU0J_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1217620.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10212..10370)
                     /locus_tag="ACHU0J_003034"
     CDS             complement(10212..10370)
                     /locus_tag="ACHU0J_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1217621.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2616 a         2609 c         2716 g         2513 t
ORIGIN      
        1 tgttaaaaaa cgattataaa atttcccttt ctccagatac aacttaccga gagctgaata
       61 aagctgcaca gagtagcatt acagcgccag acaggatagg agaagggaaa acatgggttt
      121 atcaacgaga tacaatggtt gaaagaggtg ataacagtgg tgttcatcag tatggtccag
      181 ctgaacattt cactcacatt atatctgaca aaccgtcccc aaaagataag tatgttgcat
      241 atgctattaa cattcctgac tatgagctag cagccgatgt atataatatt aacgtgacgt
      301 caccttccgg acagcaagaa acatttaaaa tactaatcaa tccagaacat ctacggcaaa
      361 cacttgagcg taaatctctt actgctgttc agaaatcaca atgtgaaatc atcaccccca
      421 aaaaacctgg cgaagcgatt cttcatgctt ttaatgccac ctaccagcaa atcagggaaa
      481 atatgtctga gtttgcacgt tcccattatg ggtatataca aatccctcca gtgacaactt
      541 tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt cacgcttatc
      601 aacccgaaga tctttgtacc atccataatc caatggggga tttgcaggat tttatcgcat
      661 tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattatacc tttaacttta
      721 tgggaatcgg gggaagtggt aaaaatgacc tggattatcc ctctgctgat atacgagcga
      781 agatcagtaa agatatagaa agtggtatcc ctggctattg gcaaggtcag gttttgattc
      841 cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa gatatacatc
      901 tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa tggaaaaacc
      961 tccatgcatt aaaagaaaag agtttaaatg gaatgcctaa aacaaccccc aaaagtgacc
     1021 aggttattat gttgcaaaat caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt
     1081 tacgttatga tgcagcaaaa cactcaaaac atgaacaaat agaaagatca ataaccccac
     1141 cgcttaaaaa ttataatgag cgattacaca atactaactt atttaaccca aaatatcatg
     1201 aaaaaaccgt tatgaattac atggaatatc tggtaacttg tcagttgaat gaagaacaaa
     1261 tgtcatcgct gctttatgaa agagatgatt taagcgccat tgatttttca ttgctcatga
     1321 agacgataaa agccttttca ttcgggggag atctcctaac ccttgcatca aaaccgggtt
     1381 ccacaatctc aagcatccca tcagaaagac ggatattgat taacattaac cacgattttc
     1441 ctaacaatgg caatcttttc aatgactttc tatttaacca tcaacaagat gaacaattag
     1501 caatggcata tatggccgct ctcccgttca gcaggccttt agtttactgg gatggccagg
     1561 tattaaaatc aacgactgaa attaaaaatt atgatgggcc cacgcgtgtc ggtggtgagg
     1621 cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc cacgcattat
     1681 atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact aaaaacgttc
     1741 tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat ggactagtta
     1801 taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg caacctggaa
     1861 tatataaaaa catggggagt aatagcgtta acattattat taataatacc cgaaaaatta
     1921 tccccccttg taaagtattt acgcttagag gcggatctct aaatatcaat attcctaggc
     1981 gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat ttataattta
     2041 atgttatatc tgccccgata aaacggggca gataatatgt ttagtttact aacggtcatt
     2101 ttgtagtgaa gccatttact gttttttatc gaccagataa tctgttctct aatgttaact
     2161 ccccctaacc tgttgcttta gttattcatt tcctgtctca ctttgcctta ataccctacg
     2221 ttaaatgtta ctaatttgtt gcttttgatc acaataagaa agcaatatgt cgcttttgtg
     2281 cgcatttttc agaaatgtag atatttttag attatggcta cgaaatgagc atcgccatgt
     2341 caccctacat ctcataagag gatcgcttct gatgaatgca ctgaccgccg tacaaaataa
     2401 cgctgtcgat tcaggccagg actatagcgg attcactctc atcccgtcgg cgcaatcccc
     2461 gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa cagtttctcg aacaggttgc
     2521 cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg taggcaaaat
     2581 tcttgacgat ctgtgtgcga atcagctgca accattgctg ttgaagaccc tgctaaaccg
     2641 tgctgaaggt gcgctgttga tcaatgcggt aggtatcgat gatgtggcgc aggcagatga
     2701 gatggtgaag ctggcaacgg cggtagcgca tctgattggt cgctcaaatt tcgatgcgat
     2761 gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc gataactcag acagctatct
     2821 gcgtcagccg caccgcgtga tggaactgca caacgacggc acgtatgtcg aagagatcac
     2881 cgattacgtg ctgatgatga aaatcgatga ctgggaacat ctggaccact atttccgcca
     2941 tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga
     3001 tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga
     3061 ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc tttccgatgc
     3121 cattgaaacc agcaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat
     3181 aaacaacctg ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg
     3241 tgaactgatg cgtcagcgtg gctatttcgc ttacgccact caccactacc agacgcatca
     3301 gtaagcgcaa aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg
     3361 gcatgtcgac cgccatgcaa ctgattgatg tttatccgga tgcccgcatt gcgttgctgg
     3421 aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg atccatgccg
     3481 gggtctatta cacgcccggc agcctgaagg cgcagttttg cctggcgggg aaccgtgcca
     3541 ccaaagcctt ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag atgctggtcg
     3601 ccacgtctca gctcgaaatg gaacggatgc gcgccttatg ggaacgcaca gcggcgaacg
     3661 gtatcgagcg cgagtggata aacgccgtgg aactgcgcga gcgcgaaccg aatatcaccg
     3721 ggcttggtgg catttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtgacggcgg
     3781 caatggcaaa aatcttccag gccagaggcg gcgagattat ttataacgcc gaagtcagcg
     3841 ccctcagtga gcataaaaac ggcgtggtga tacgcacccg tcagggcggt gaatatgaag
     3901 catcgacgct gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggcc
     3961 tcgaaccggg ctttattatc tgcccgttcc gtggcgagta tttccgcctt gcgccggagc
     4021 ataaccagat tgttaaccac ctgatttacc ccattcccga ccccgcgatg ccatttttgg
     4081 gcgtgcatct cacccgcatg atcgacggca gcgtgaccgt cgggccaaac gcggtgctgg
     4141 ctttcaaacg cgaaggctat cgcaagcgcg acttctcatt tagtgacacg ctggagattt
     4201 tgagctcgtc ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga
     4261 tgaaaaactc gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc
     4321 tttcgttaag cgatctccag ccctggcccg ccggtgtgcg ggcgcaggca gtatcgccgg
     4381 acggcaagct gattgacgat tttctgtttg tcaccacccc acgcacgatc cacacctgca
     4441 atgcgccctc cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg
     4501 tacaaacgct gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg
     4561 tggatgcctt acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa
     4621 cttaacgaca gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc
     4681 aacaatggtg aagtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg
     4741 ccgaaaatgg gcgcagatga aacccgcgcc gctatcgacg ccgccaaccg cgccctgccc
     4801 gcctggcgtg cgctcaccgc caaagaacgc gccaccattc tgcgcaactg gttcaatttg
     4861 atgatgcagc atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg taaaccgctg
     4921 gctgaagcga aaggcgaaat cagctacgcc gcctccttca ttgagtggtt tgctgaagaa
     4981 ggcaaacgta tttatggcga caccattcct ggtcatcagg ccgataagcg cctgattgtt
     5041 atcaagcagc cgattggcgt caccgccgcc atcacgccgt ggaactttcc ggcggcgatg
     5101 attacccgta aagccggtcc ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc
     5161 agtcagacgc cgttctctgc gctggcgctg gcggaactgg cgattcgcgc gggcattccg
     5221 gctggcgtgt ttaacgtggt caccggttcg gcaggcgtgg tcggtaatga actgaccagc
     5281 aacccgctgg tacgcaaact gtcgtttacc ggttagaccg agattggccg ccagttaatg
     5341 gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc tgggcggcaa cgcgccgttt
     5401 atcgtctttg acgatgccga tctcgataaa gccgtggaag gcgcgctggc ctcgaaattc
     5461 cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat
     5521 gaccgctttg ccgaaaaatt acagcaggcg gggagcaaac tgcacatcgg cgacgggctg
     5581 gagaaaggcg tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag
     5641 catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa agcgcacgaa
     5701 cgcggcggca acttcttcca gccaaccatt ctggtggacg ttccggccaa cgccaaagtg
     5761 tcgaaagaag agacgttcgg ccccctcgcc ccactgttcc gttttaaaga tgaagccgat
     5821 gtgattgcac aagccaatga caccgagttt ggtcttgccg cctatttcta cgcccgtgat
     5881 ttaagccgcg tcttccgcgt cggcgaggcg ctggagtacg gcatcgtcgg catcaatacc
     5941 ggcattattt ccaatgaagt ggccccattc ggcggcatca aagcctcggg tctgggtcgt
     6001 gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt
     6061 taactggaga atgcgaagga acagcaataa agagttaatg cagcgccgca gtcaggcgat
     6121 tccccgtggc gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt
     6181 gtgggacgtt taaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac
     6241 cgggcacctg catccgaagg tggtggccgc ggtggaagcg cagttgaaaa aactgtcgca
     6301 cacctgcttc caggtgctgg cttacgagcc gtatctggag ctgtgcgaga ttatgaatca
     6361 gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt acgaccggtt ccgaagcggt
     6421 ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag
     6481 cggcgagtat cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta
     6541 ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct
     6601 gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc
     6661 cgcgccggaa gatatcgccg ccatcatgat tgagccggtt cagggcgaag gcggtttcta
     6721 cgccgcgtcg ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat
     6781 gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga
     6841 gcagatgggc gtggcgccgg atcttaccac ctttgcgaaa tcgatcgcag gcggtttccc
     6901 gctggcgggc gtcaccgggc gcgcggaagt gatggacgct gttgcaccgg gcggtctggg
     6961 cggcacctat gccggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt
     7021 cgagcaggaa aatctgctgc aaaaagccaa cgatctgggg cagaagctaa aagacggatt
     7081 gttggcgata gccgaaaaac acccggagat cggcgacgta cgcgggctgg gatcgatgat
     7141 tgccatcgag ctgtttgaag atggcgatca cagcaaaccg gacgcaaaac tcaccgccga
     7201 gatcgtggca cgcgcccgcg ataagggcct gattcttctc tcctgcggcc cgtattacaa
     7261 cgtgctgcgc atccttgtac cactcaccat tgaagacgct cagatccgtc agggtctgga
     7321 gattatcggt cagtgttttg atgaggcgaa gcagtagcac cgtccccatg caggaggcga
     7381 cgctgcgcgt cttgtccggc ctacgagaat ccaagtcgga taaggcgttt acgccgcatc
     7441 cgacaatctg tacgtgaaca ggaataaatc tatgtcggcc ggataaggcg gagccgctct
     7501 ccggcaaaaa gaatcaataa caattaaacg cgtgacccga cgcgggaaat gtcggggcgc
     7561 tctcccaagt gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacacga
     7621 gttaggcggc gggctgaaat cacgccacgt caccatgttg tctattgccg gtgttatcgg
     7681 cgcaagtctg tttgtcggtt ccagcgtcgc catcgccgaa gcggacccgg cggtattact
     7741 ggcctatctg ttcgccggat tactggtggt tatgattatg cggatgctgg cggaaatggc
     7801 agttgccacg cccgataccg gttcgttttc cacctatgcc gataaagcca ttggccgctg
     7861 ggcgggctat accatcggct ggttgtactg gtggttttgg gtactggtta tcccactgga
     7921 agccaacatc gccgccatga tcctgcactc gtgggttcca ggcattccca tctggttatt
     7981 ttccctcgtc attaccctcg ccttaactgg cagtaattta ttaagcgtta aaaactacgg
     8041 cgaatttgag ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct
     8101 tggtgcagtc gcaattagcg gtttttaccc ttatgccgaa gtgagcggga tctcaagatt
     8161 gtgggatagc ggcggcttta tgcccaacgg tttcggtgcg gtattaagcg cgatgttgat
     8221 caccatgttc tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacat
     8281 gccggaaaaa catattgtcc gcgccaccaa ctcggttatc tggcgtattt ctatcttcta
     8341 tttgtgctct atttttgtcg tcgtggcatt aataccgtgg aatatgccgg ggctgaaagc
     8401 cgttggatct tatcgctcgg ttctggaatt gctcaatatt ccccatgcga aattaatcat
     8461 ggactgtgtg atattacttt ccgtaaccag ttgcctgaac tcggccctgt ataccgcctc
     8521 aaggatgctc tactccttaa gccgtcgcgg tgatgctccc gcggtaatgg gtaaaatcaa
     8581 ccgcagtaaa accccgtacg tggcggtgtt actctccacc ggcgcagcat tcttaacggt
     8641 ggtggtgaac tattacgcgc ctgcgaaggt atttaaattt ctgatcgaca gctccggcgc
     8701 tatcgccctg ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gcaaaattct
     8761 gcgagcagaa ggaagcgaaa ttcgcttgcg aatgtggctt gatccctggc tcacctggct
     8821 ggtcatcggc tttattacct ttgtgttggt agtgatgcta ttccgtccgg cgcaacagtt
     8881 agaagtgatc tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc
     8941 tcgctggaaa aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgataatg
     9001 atcaggagtc acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat
     9061 attattcgcg gtaattttca accggatgaa aaattacgga tgagtttact gacatcgcgt
     9121 tatgcacttg gcgttgggcc gttgcaggaa gcactttctc aactggtggc ggaacggctg
     9181 gtcacggtgg tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc
     9241 gatattttcg acgcccgcgc caatatggaa gcgatgctgg tcagtctggc gattgcccgc
     9301 ggtggcgatg aatgggaggc agacgttctc gcaaaagcgc atctgctcag taagctggaa
     9361 gcctgtgacg ccagcgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat
     9421 acagcaattg tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt
     9481 gatctggcgg cgcgttatcg atttatctgg ctgcggcgaa cggtgctttc ggtggaaatg
     9541 ctggaggata aacacgatca gcaccagacc ctgactgcgg cggtactgcc gcgagataca
     9601 gcgcgcgcca gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag
     9661 gcgatggctg gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt
     9721 atccgggctt tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt
     9781 accgtagacc tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg
     9841 gctggcagtg gctggtgtcg ccgttttcac ctgatcatca acactggcaa taccggaaat
     9901 attccccacc gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac
     9961 cgtcgctttg ccgtcggcaa tttgaatgtt cactttatcg gcatcaggta taccggtttt
    10021 gctcagatgc tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc
    10081 atcccagagt ttttctccgg catctttaac aaaattgaac agacccatag ctacctcatt
    10141 cgttaacgga aacactcaga aagtgtagca gaggaacgcg gcgcgttaac gccgcggggg
    10201 tgagcacagg tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac
    10261 ccaagcagtg tcagcagaat attaataatg aacgcccaac cgaacccttt accgagcagc
    10321 acgccgatcg gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc
    10381 tataagaaaa ttactcattg tttaaaaaga gattttatct cttaagcgta gtaattttag
    10441 cggagcctgc cagt
//
LOCUS       DAZPDE010000132        10429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPDE010000132 DAZPDE010000000
VERSION     DAZPDE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.132"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHU0J_003035"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHU0J_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1217622.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHU0J_003036"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHU0J_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1217623.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHU0J_003037"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHU0J_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1217624.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHU0J_003038"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHU0J_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1217625.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHU0J_003039"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHU0J_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1217626.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHU0J_003040"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHU0J_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1217627.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHU0J_003041"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHU0J_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1217628.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHU0J_003042"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHU0J_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHU0J_003043"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHU0J_003043"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1217629.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHU0J_003044"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHU0J_003044"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1217630.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHU0J_003045"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHU0J_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1217631.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2554 a         2656 c         2612 g         2607 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggtaa aaaaagcac
//
LOCUS       DAZPDE010000133        10270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPDE010000133 DAZPDE010000000
VERSION     DAZPDE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.133"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            286..375
                     /locus_tag="ACHU0J_003046"
                     /pseudo
     CDS             286..375
                     /locus_tag="ACHU0J_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            677..1672
                     /gene="pgl"
                     /locus_tag="ACHU0J_003047"
     CDS             677..1672
                     /gene="pgl"
                     /locus_tag="ACHU0J_003047"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1217632.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1713..2666)
                     /locus_tag="ACHU0J_003048"
                     /pseudo
     CDS             complement(1713..2666)
                     /locus_tag="ACHU0J_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2850..3902
                     /locus_tag="ACHU0J_003049"
     CDS             2850..3902
                     /locus_tag="ACHU0J_003049"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR1217633.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3978..5411
                     /locus_tag="ACHU0J_003050"
     CDS             3978..5411
                     /locus_tag="ACHU0J_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR1217634.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5594..7774
                     /locus_tag="ACHU0J_003051"
     CDS             5594..7774
                     /locus_tag="ACHU0J_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1217635.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7915..9198)
                     /locus_tag="ACHU0J_003052"
     CDS             complement(7915..9198)
                     /locus_tag="ACHU0J_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1217636.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9333..>10270)
                     /locus_tag="ACHU0J_003053"
     CDS             complement(9333..>10270)
                     /locus_tag="ACHU0J_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1217637.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2512 a         2385 c         2574 g         2799 t
ORIGIN      
        1 ttagtcttga gtaagatact acaacatctc caactatttt agccagcctg actacttgac
       61 ttggtaatat ttgttgctaa taatcatgta taaaataagc ttatatacat agtaacttca
      121 gcaaaggatt tagtaatgat caaaatcata tctcaatgta ctgttttttt ctttaaaaaa
      181 acagtacatt ctattttata atgactgtgt attcccatgg aaggagatca cccaattctc
      241 ccccccgact tttgacgcag tgtcttgtta acagggggat tgaccatgct tattggctat
      301 gtacgcgtat caacaaatga ccagaatacc gatctacaac gtaatgcgtt tatttgtgcc
      361 ggaagtgagt tgattgttgc gcgaatggga agttggccag aagttgttat ggcatagtgt
      421 ttacggtcgt tgatacaggt aaacaaaagc ggggagagtc atactccctg aatagtcgcg
      481 aaaatatctg tggcattgtc aaattcgtgt cggtgactaa tattttagta atatgccatt
      541 cgaaatggag tgctaactta ttgatttaat tgatgtgcga tattgtattt tatcgtgttt
      601 tgttgtctgt tttttatcca gtaagtgttg ttatatgtat gaaagataag ggatttatca
      661 tttttgaggt gtgttcatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat
      721 tcacgtctgg aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc
      781 ggggcaggtg cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg
      841 ccctgagttt cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc
      901 cgcagagtct gtgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt
      961 tgtctttgta ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct
     1021 gccagtgggc gtcgtcgatg tagtcgaggg gctggacggt tgccattccg ccaatatctc
     1081 accggacaac cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac
     1141 ggtcagcgat gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccgttgaagg
     1201 ggccggcccg cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga
     1261 gttaaacagc tcagtggatg tctgggaact gaaagatccg cacggcaata tcgaatgtgt
     1321 ccagacgctg gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca
     1381 tatcaccccg gatggtcgcc atttatacgc ctgcgaccgt accgccagtc tgattaccgt
     1441 tttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caacggaaac
     1501 ccagccgcgc ggcttcaatg ttgattacag cggcaagtat ctgattgccg ctgggcaaaa
     1561 atctcaccac atctcggtat acgaaattgt tggcgagcag gggctactgc atgaaaaagg
     1621 ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat
     1681 ttaaccggcg cagtctctcc tgcgccggtg tattaactta tctcctgtaa cgcatgtctc
     1741 tggcgttcga cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc
     1801 aaggaaaaaa agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc
     1861 aatgtccgac gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca
     1921 tggggcattg ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat
     1981 gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggctagc
     2041 tgttgttcta tcaacaatcg gttgccgctg gaggaacgta cgccaatcat tttgtaaccc
     2101 actaactcct gccattctac aagctacttt ttggccaatg gatggtcacg ccgacaggcc
     2161 agcacgaatg gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc
     2221 atgtttatgc caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct
     2281 tgttccagaa tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc
     2341 ggtaaaaaat aaaatactgc ggtcggaata cacgctaatg tcaccatacc acgatgataa
     2401 gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt tctttattaa ttcccgcgcc
     2461 tccgggagca acctttttcc tgcttttgtt aacgtaacct tgcgagttgt acgctcaaat
     2521 agttgaatat gtaaatcttc ctccatcttt tttattcttc gcgttaatgc aggttgcgta
     2581 atattgagta atttagcggc attgttaaat gaactggact ctgccagtat gacaaatgcc
     2641 ttcatacttg ataattcatg cttcattatt actccggaaa atggaagcga cgattttggg
     2701 gggctggtcg ttaaaaattt taactgcatt tagccaactt aaattaatga aaaaatgtta
     2761 ttaatcgttg agctaaagtc attagagatg ctttgtcctt aatgtaacca tatcgcaata
     2821 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
     2881 gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc
     2941 gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg
     3001 gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg
     3061 ctgatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc
     3121 ctaactgcgg caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg
     3181 cagcgacttc gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg
     3241 aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg
     3301 gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa
     3361 ctggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct
     3421 gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt
     3481 atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc
     3541 ggctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac
     3601 ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc
     3661 attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat
     3721 tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg
     3781 aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt
     3841 tacgcgcatc tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct
     3901 gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag
     3961 aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga
     4021 tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc
     4081 tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg
     4141 tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg
     4201 cgtttaagac caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct
     4261 cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaactt attgcctatc
     4321 tgctgattgg taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg
     4381 atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac
     4441 cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg
     4501 tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct
     4561 ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc
     4621 aaattagctg gggtggatgg gcgctggccg ccggattgcc gggcatcatt atgctgctgg
     4681 tcaccccgct ggtaatttac accatgtatc caccagaaat taaaaaggtg gataacaaaa
     4741 ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa aatccgcgaa aaaatgctgc
     4801 tcggtgtctt tgtgctggcg ctactgggct ggattttcag taagtctctg ggggttgatg
     4861 aatccactgt ggcaatcgtt gttatggcga ccatgctgct gctgggtatc gttacctggg
     4921 aagacgtggt taaaaataaa ggcggctgga ataccttaat ctggtacggc ggtattatcg
     4981 gcttaagctc cttattatcg aaagttaaat ttttcgaatg gttagctgaa gtctttaaaa
     5041 ataacctggc atttgatggt cacggtaacg ttgctttctt cgttattatt ttcctcagca
     5101 tcatcgtgcg ttatttcttc gcttccggta gtgcctatat cgttgctatg ttacctgtat
     5161 ttgccatgct ggcgaacgtc tccggcgcac cgttaatgtt aaccgcgctg gcactgttgt
     5221 tctccaactc ctatggcggc atggttactc actatggcgg cgcggcaggt ccggtcatct
     5281 ttggcgtggg ttacaacgat attaaatcct ggtggttggt cggtgcggta ctgacgatat
     5341 taaccttctt ggtgcatatc accctcggcg tgtggtggtg gaatatgctg atcggctgga
     5401 acatgctgta aatatactcg tcatacttca agttgcatgt gctgcgtctg cgttcgctca
     5461 ccccagtcac ttacttatgt aagctcctgg ggattcactc acttgtcgcc ttcctgcaac
     5521 tcgaattatt tagagtatat ccatttatta tctttctgcg cacttcacgg tgcgcagata
     5581 tctggagcat ttgatgatca agttatctga aaaaggcgtg tttctcgcca gtaataacga
     5641 aataattgcc gaagaacatt tcaccggcga aattaaaaaa gaagaagcaa aaaaaggcac
     5701 tattgcctgg tctattctct cttctcataa tacgtccgga aatatggata aacttaaaat
     5761 taagtttgat tcattagcct ctcacgatat tacctttgtt ggtattgtac agaccgctaa
     5821 agcgtccgga atggaacgtt tcccgctgcc gtatgtgctg accaactgcc ataactcact
     5881 ctgcgccgtc ggcggcacca ttaacggtga tgaccatgtt tttggtttat cggcagctca
     5941 gcgttatggc ggtatttttg tgcctccgca tattgcggtc atccatcaat atatgcgtga
     6001 gatgatggct ggcgggggca aaatgatcct cgggtcagac agccacaccc gttacggtgc
     6061 attagggaca atggcagtcg gtgagggtgg cggtgagttg gtaaaacagc tgcttaatga
     6121 cacctgggat atcgactatc cgggcgtggt tgcggtgcat ctgaccggaa aacctgcgcc
     6181 gtatgtggag ccacaggatg tggcgctggc tatcattggc gcggtgttca aaaacggtta
     6241 cgtcaaaaac aaagtcatgg agttcgttgg accgggcgtt agcgcgctct ctaccgattt
     6301 ccgtaacagc gttgacgtga tgaccacgga aaccacctgt ttaagctccg tctggcaaac
     6361 tgatgaagaa gtgcataact ggctggcgct gcacggtcgc ggtcaggatt actgccagct
     6421 taaccctcaa ccgatggcgt actacgatgg ctgcatcagc gttgatttaa gcgccatcaa
     6481 accaatgatt gcgctgccgt tccacccgag caacgtgtat gaaatcgaca cactgaacca
     6541 gaacctgacc gacattctgc gtgagattga aattgagtcc gaacgcgtgg cgcacggtaa
     6601 agccaaactc tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc agcaggggat
     6661 tatcgcgggc tgttctggcg gtaactacga aaacgtcatc gcggcggcga atgcactgcg
     6721 cggtcaatcc tgtggcaatg acaccttctc gctggcggtt tacccgtcat cacagccggt
     6781 gtttatggat ctcgccaaaa aaggtgtggt agcagatttg attggcgcag gcgcaatcat
     6841 cagaaccgca ttctgtggcc catgctttgg cgcgggcgat acgccaatca acaacggttt
     6901 gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc cagccaatgg
     6961 gcagatgtca gcggtggcgt tgatggacgc tcgttctatc gctgcgactg cggcaaacgg
     7021 tggctattta acctctgcca gcgaacttga ttgctgggac aacgtgccgg agtacgcctt
     7081 cgatgtaacg ccgtataaaa accgtgttta tcagggcttt gtgaaagggg caactcagca
     7141 accgctgatt tacggaccga acattaaaga ctggccggaa ttgggtgcgc tgactgacaa
     7201 tatcgtcctg aaagtgtgct cgaagatcct cgacgaagtc accaccaccg acgaactgat
     7261 tccttccggt gaaacctctt cttatcgttc aaatccgatt ggtctggcgg ggaatgtcag
     7321 cgagctgaca gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc atattgatcc
     7381 gctgcaaact gaaattggta gcatggtcta tgcggtgaaa ccaggcgatg gttctgcgcg
     7441 tgaacaggcg gcgagctgcc agcgtgtgat tggcggtctg gcgaatattg ccgaggagta
     7501 cgcgactaaa cgctaccgtt ctaacgtcat caactggggg atgttaccgc tgcagatggc
     7561 ggaagtgcca acctttgaag tgggggatta catttacatc cctggcatta aagcggcgct
     7621 ggataatccg ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga
     7681 aattacgctt tacatggaaa gcctgacggc tgaagagcgc gagattatca aggcgggtag
     7741 tttgattaac ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca
     7801 ttcggcactt attgtcggat gcgatgctgg cgcatcttat ccgacctaca aatcgcatcg
     7861 aaccgtaggc cggataaggc gtttacgccg catccggcaa atagttaata gctcttactt
     7921 cttctcctct gcaaccactt tgctgcccac gccgcggtta ttgtattccc acatgcggtt
     7981 gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta tcatctacgc tgccggtatt
     8041 acccgcaaac ggacgattag agatcaccgc atcggcccac ggtttagccg tgttaaaacc
     8101 ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg gtattagcat caacatccag
     8161 cgagcggccc agttgcgcca cgccatcacc ggaagcattg aaacggctgt ttacggcgag
     8221 gaaaccgtaa tagatgttag acagcgtagc cggtgcaaac acatatgctt cttgctgggt
     8281 acgtgagttc accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac
     8341 gatatccaca tccccttcaa tatagctgtt ggtcaccagc gtacgcggct gacggttggt
     8401 ttccagacgg ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat
     8461 gttaacgttg ttaatctgta cttggtcacc gtcagtacgc agtgctaccg ccggatggtt
     8521 acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc agattttgca gttgcagtcc
     8581 attgttttgt gaccagaaga ccgcagaaca cagaacaccg atactgtcgc tgcgtttgct
     8641 ctggcagcta tcgtacatat accacgctgg tttacctggc atatatttgc cgcgcgggtt
     8701 gacgtcgtga cgccagtcgg cagggctcat gcccccatca agggaaagcc caatcttcac
     8761 atcaatcggt ttttcacctg taccgtacag agtgattcca cctggagcag cagggacata
     8821 caccgttccc tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtgcgctt
     8881 gataattgcc gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtacccgc
     8941 cgggccgaca acaaagtcag gttgcgcagg cagggttatc ggggacggat tccacgctgc
     9001 agcacctggt gtcagggatg caaaatagtg ttgcgcatcg aaattcagcg cttcttttgc
     9061 cgacagaatc gggcgagaag aggtaccagg cgcagtttga tcagaaggac gttgatcggg
     9121 tggtgttgag ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga
     9181 aactgaaaat gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa
     9241 gcctgctttt ttatactaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
     9301 cgaacaggtc attatcagtc aaaataaaat cattatttga tttcaatttt gtcccactcc
     9361 ctgcctctgt catcacgata ctgtgatgcc atggtgtccg acttgtgccc gagaagatgt
     9421 tgagcaaact tatcgcttat ctgcttctca tagagtcttg cagacaaact gcgcaactcg
     9481 tgaaaggtag gtggatcccc ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa
     9541 taccttgata ctgtgccgga tgaaagcggt tcacgacgag tagatgcaat tatggtttct
     9601 ccgccaagaa tctctttgca tttatcaagt gtttccttca ttgatatccc gagagcatca
     9661 acatgcaatg ttgtagggat ggcaattttt acgcctgttt tgctttgctc gacataaaga
     9721 tatccatcta cgatatcaga ccacttcatt tcgcataaat caccaactcg ttgcccggta
     9781 acaacagcca gttccattgc aagtctgagc caacatggtg atgattctgc tgcttgataa
     9841 attttcaggt attcgtcagc cgtaagtctt gatctcctta cctctgattt tgctgcgcga
     9901 gtggcagcga ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg
     9961 ctcagtgttg atctgattaa cttggctgat gccgccttgc cctcgtctat gtatccattg
    10021 agcattgccg caatttcttt tgtggtgatg tcttcaagtg gagcatcagg cagccccctc
    10081 cttattgctt taattttgct catgtaattt atgagtgtct tctgcttgat tcctctgctg
    10141 gcgaggattt tttcgtagcg atcaagccat gaatgtaacg taacagaatt atcactgttg
    10201 attctcgctg tcagaggctt gtgtttgtgt cctgaaaata actcaatgtt ggcctgtata
    10261 gcttcagtga
//
LOCUS       DAZPDE010000134        10188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPDE010000134 DAZPDE010000000
VERSION     DAZPDE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.134"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHU0J_003054"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHU0J_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1217638.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHU0J_003055"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHU0J_003055"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR1217639.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHU0J_003056"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHU0J_003056"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1217640.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHU0J_003057"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHU0J_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1217641.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHU0J_003058"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHU0J_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1217642.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHU0J_003059"
     CDS             4025..4615
                     /locus_tag="ACHU0J_003059"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1217643.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHU0J_003060"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHU0J_003060"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1217644.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHU0J_003061"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHU0J_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1217645.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHU0J_003062"
     CDS             complement(6551..6658)
                     /locus_tag="ACHU0J_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR1217646.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHU0J_003063"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHU0J_003063"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1217647.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHU0J_003064"
     CDS             7648..7875
                     /locus_tag="ACHU0J_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1217648.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHU0J_003065"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHU0J_003065"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1217649.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>10188)
                     /locus_tag="ACHU0J_003066"
     CDS             complement(9447..>10188)
                     /locus_tag="ACHU0J_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217650.1"
                     /translation="MPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRA
                     ILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDL
                     QSDNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSD
                     RIKAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYL
                     PPELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2365 a         2631 c         2629 g         2563 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcata
//
LOCUS       DAZPDE010000135         9937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPDE010000135 DAZPDE010000000
VERSION     DAZPDE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.135"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHU0J_003067"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACHU0J_003067"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1217651.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHU0J_003068"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACHU0J_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1217652.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHU0J_003069"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACHU0J_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1217653.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHU0J_003070"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="ACHU0J_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1217654.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHU0J_003071"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="ACHU0J_003071"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1217655.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHU0J_003072"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="ACHU0J_003072"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1217656.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHU0J_003073"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="ACHU0J_003073"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1217657.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHU0J_003074"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="ACHU0J_003074"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1217658.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHU0J_003075"
     CDS             complement(8990..>9937)
                     /gene="lpfD-O113"
                     /locus_tag="ACHU0J_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1217659.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2587 c         2295 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttcct
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAZPDE010000136         9937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPDE010000136 DAZPDE010000000
VERSION     DAZPDE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.136"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            421..1035
                     /gene="evgA"
                     /locus_tag="ACHU0J_003076"
     CDS             421..1035
                     /gene="evgA"
                     /locus_tag="ACHU0J_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1217660.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1040..4633
                     /gene="evgS"
                     /locus_tag="ACHU0J_003077"
     CDS             1040..4633
                     /gene="evgS"
                     /locus_tag="ACHU0J_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1217661.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4689..5834)
                     /gene="yfdE"
                     /locus_tag="ACHU0J_003078"
     CDS             complement(4689..5834)
                     /gene="yfdE"
                     /locus_tag="ACHU0J_003078"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1217662.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5908..6852)
                     /gene="yfdV"
                     /locus_tag="ACHU0J_003079"
     CDS             complement(5908..6852)
                     /gene="yfdV"
                     /locus_tag="ACHU0J_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1217663.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6922..8616)
                     /gene="oxc"
                     /locus_tag="ACHU0J_003080"
     CDS             complement(6922..8616)
                     /gene="oxc"
                     /locus_tag="ACHU0J_003080"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1217664.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8670..9920)
                     /gene="frc"
                     /locus_tag="ACHU0J_003081"
     CDS             complement(8670..9920)
                     /gene="frc"
                     /locus_tag="ACHU0J_003081"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1217665.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2954 a         2242 c         2040 g         2701 t
ORIGIN      
        1 tccactatta tctctcattt cgcatagatg aaatttatga attgaatgaa aagagtattc
       61 attcttcata tcaaacacag tgcatgtgtt gcacattatc ccaggaacgt tggaggccta
      121 atttaaatga ggtgcaaaaa aaacaacaga ggactaaacc gtggcttttg caatacaatt
      181 cttacgcctg taggattagt gagaagactt atagtgctaa cttgaaacta taaatcatcg
      241 gtataatccc tgattttatt gttgacattt catttatgcc gactatttat atggtatact
      301 tgtcgaatta tcttaaagga aactcagatt ttcttatttt tattgagaaa atgagatgac
      361 gccttatgtc tgtattacta cagggagaag ggagatgctt cattgcaaag ggaataatct
      421 atgaacgcaa taattattga tgaccatcct cttgctatcg cagcaattcg taatttattg
      481 atcaaaaacg atattgaaat cttagcggag ttaactgaag gcggaagcgc cgttcagcgg
      541 gtggaaacac ttaagcctga tatcgtcatc attgatgtcg atatccccgg agttaacggt
      601 atccaggtgt tagaaacgct gaggaagcgc caatatagcg gaattattat tatcgtctcc
      661 gctaaaaatg accattttta cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg
      721 agtaaaaaag aaggcatgaa caatatcatt gcggctattg aagctgcaaa aaatggctac
      781 tgctatttcc ccttctctct caaccggttt gttggaagtt taacgtccga ccagcaaaaa
      841 ctcgactcct tatcgaaaca agaaattagt gtcatgcggt atattcttga tggcaaggat
      901 aataatgaca ttgcggaaaa aatgttcatc agcaacaaaa ctgtcagcac gtataaaagt
      961 cgcctgatgg aaaaattaga atgtaaatca ctgatggatc tttacacatt cgcgcaacgt
     1021 aacaaaatcg gctaatcaca tgaagttttt accctatttt tttcttctct gttgtggtct
     1081 ttggtcgacc ataagtttcg cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa
     1141 caaccgtgtc acacttgatc cagtacgtct gagcaacaag gaattacgtt ggttagcgag
     1201 caaaaaaaat cttgtgattg cagtacacaa gtcccaaacg gctaccttgt tgcataccga
     1261 ttcgcagcaa caggttcgtg gtattaatgc tgattattta aatcttttaa aaagagcgtt
     1321 aaatatcaaa ttaacactcc gggaatacgc agatcatcaa aaagcaatgg acgcgcttgc
     1381 agaaggtgaa gtcgatatag tgttatcaca tttagttact tcgccgcctc ttaataatga
     1441 cattgctgca accaaaccat tgataattac ctttccggcg ctggtaacca cccttcacga
     1501 ctcaatgcga ccgcttacct caccaaaacc agtaaatatt gctcgggtag caaattaccc
     1561 cccagacgag gtaattcatc aatcatttcc aaaagcaaca attatctctt ttacaaattt
     1621 atatcaggca ttagcatccg tctcagctgg gcacaatgat tactttattg gtagtaacat
     1681 cattaccagc agtatgattt cccgctattt cactcactcc ttaaatgtag tgaaatatta
     1741 taactcgccg cgtcaatata attttttctt gaccagaaaa gaatctgtca ttcttaatga
     1801 agtactcaat agatttgttg atgctttaac aaatgaagtt cgctatgaag tatcacaaaa
     1861 ttggcttgat acaggaaacc tggcctttct gaacaaacca ttagaactca ctgaacatga
     1921 aaaacagtgg attaagcagc atcccaattt aaaggtgctg gaaaatcctt actcgccccc
     1981 ctattctatg acggatgaaa atggctcggt tcggggcgtt atgggggaca ttcttaatat
     2041 tattaccttg caaacaggtt taaatttttc tccgatcacc gtttcacaca atatccatgc
     2101 tggaacacaa cttagccccg gaggatggga tataatacct ggcgctattt atagtgaaga
     2161 tcgagaaaat aatgttttat ttgctgaagc cttcataaca acgccttacg tttttgtcat
     2221 gcaaaaagcg cctgacagtg aacaaacatt aaaaaaagga atgaaagttg ccattccata
     2281 ttattatgag ctgcattcgc aattaaaaga gatgtatccg gaggttgaat ggatacaggt
     2341 cgataatgcc agcgctgcat ttcacaagat taaggaaggt gaacttgatg ctctggtcgc
     2401 gacacagcta aattcgcgtt acatgatcga tcattactat cctaatgaac tttatcattt
     2461 tcttattcct ggcgttccga atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga
     2521 acttaaggat attattaata aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct
     2581 gacggaaaaa tggattaaaa tgcccaatgt gaccattgac acatgggacc tatatagcga
     2641 gcaattttat attgttacga cattatccgt tttattagtt ggcagtagcc ttttatgggg
     2701 attctacctg ttacgctcag ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca
     2761 aatatcattc cgaaaagcac tctcggattc cttaccgaat ccaacttatg ttgtaaactg
     2821 gcaaggtaat gtcattagtc ataatagtgc ttttgaacat tatttcactg cggattacta
     2881 caaaaatgca atgttaccat tagaaaacag tgattcaccc tttaaagatg ttttttctaa
     2941 tgcgcatgaa gtcacagcag aaacgaaaga aaatcgaaca atatacacac aggtatttga
     3001 aattgataat ggcatcgaga aaagatgcat taatcactgg catacattat gcaatcttcc
     3061 tgcaagtgac aatgcagtat atatttgtgg ttggcaagat attactgaaa cgcgtgatct
     3121 aattaatgca ctcgaggtag aaaaaaataa agcgataaag gctaccgtag caaaaagtca
     3181 gtttctggca acgatgagtc acgaaataag aacaccaata agctctatta tgggcttcct
     3241 ggaacttctg tcgggttctg gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc
     3301 ctacgccacc ggacaatcac tcctcggctt aattggtgaa atccttgatg tcgacaaaat
     3361 tgaatcgggt aactatcaac ttcaaccaca atgggtcgat atccctactt tagtccagaa
     3421 cacttgtcac tctttcggtg cgattgctgc aagcaaatcg atcgcattaa gttgcagcag
     3481 tacatttcct gatcattacc tggttaagat cgaccctcag gcgtttaagc aggtcttatc
     3541 aaatttactg agtaatgctc tcaaatttac caccgagggg gcagtaaaaa ttacgacctc
     3601 cctgggtcac attgatgaca accacgctgt tatcaaaatg acgattatgg attctggaag
     3661 tggattatcg caggaagaac aacaacaact gtttaagcgc tacagccaaa caagtgcagg
     3721 tcgtcagcaa acaggttctg gtttaggctt aatgatctgc aaagaattaa ttaaaaatat
     3781 gcagggcgat ttatcattag aaagtcatcc aggcatagga acaacattta cgatcacaat
     3841 cccggtagaa attagccagc aagtggcgac tgtcgaggca aaagcagaac aacccatcac
     3901 actacctgaa aagttgagca tattaatcgc ggatgatcat ccgaccaaca ggctattact
     3961 caaacgccag ctaaatctat taggatatga tgttgatgaa gccactgatg gtgtgcaagc
     4021 gctacacaaa gtcagtatgc aacattatga tctgcttatt actgacgtta atatgccgaa
     4081 tatggatggt tttgagttga ctcgcaaact ccgtgagcaa aattcttcct tacccatctg
     4141 ggggcttaca gccaacgcac aggctaacga acgtgaaaaa gggttaagtt gcggcatgaa
     4201 cttatgtttg ttcaaaccgt tgaccctgga tgtactgaaa acacatttaa gtcagttaca
     4261 ccaggttgcg catattgcac ctcagtatcg ccaccttgat atcgaagccc tgaaaaataa
     4321 tacggcgaat gatctacaac tgatgcagga gattctcatg actttccagc atgaaacgca
     4381 taaagatcta cccgctgcgt ttcatgcact agaagctggc gataacagaa ctttccatca
     4441 gtgtattcat cgcatccacg gtgcggctaa catcctgaat ttgcaaaagt tgattaatat
     4501 tagccatcag ttagaaataa cacctgcttc agatgacagt aagcctgaaa ttcttcagct
     4561 gctgaactct gttaaagaac acattgcaga gctggaccag gagattgctg ttttctgtca
     4621 gaaaaatgac taaattgcgg ctcccacaat gttctaatgt gggagctatt taccagcaca
     4681 tcttacattt atgatgagaa ctcctggcga atttgttccc catgctggtc gagcgttgcc
     4741 gctcccggca taacatgcgg gtccgcgcag ccgctgattt ttatcggatt acccggcatc
     4801 attattcctc cggcttcaat caacatattt ctcgcctgag tttgtggcaa attaatggcc
     4861 tcagccacac ttaataacgg cgcgacgggc acaccaactt catgtatttt ggctaaccaa
     4921 acttcagctg cctgcgtttt taacgttcgc tcaatatatt gtttaagaat agcctggttt
     4981 tgtacgcgta aaatattgct gctaaatcgg ggatcattaa ccagttccgt aagctccagt
     5041 gcctggcata acgcagaaaa aagcttgtca ttaccacaac aaatcgtaat cggcttatcc
     5101 tgagtatcga aaacatcaaa aggtgccatg tagggatggc gatttcccag gcgttgtggt
     5161 gacttccctg tcgcaatata tgccatcaga ccatgctcca gaaaactcag cgtggcatca
     5221 aacatcgcta tatcgacatg cgcccctctc tggctctttt cgcggccata aagtgcactc
     5281 actattccgc tgaataaata gacaccgccg catagatccg caagagaggt accaacacgc
     5341 actggcggag catcagggta ccctgtttcc atcattatcc cactcattgc ctgaatgatg
     5401 gtatcgtagg caggagcatc ttttagcgga ccggtatgtc cgaaacctga cgatgaagca
     5461 tatatgaggc gcgggttgat ttcttgtaac ctttcccatg aaaaccccag tttttccatt
     5521 gtacctaggc gaaaattctc agctaataca tcagcttgtt tgagcatatt tataaatata
     5581 cttttatcgt gatcattctt taaatcaaga accacactct ctttgccatg attaataaaa
     5641 ctgtaataga gtgactgtcc atccacatag ggaccaaatg tgcgggtatc atcaccatga
     5701 cctggtggct caactttaat tacccttgcg cccatattat aaagaagttg agttccgaaa
     5761 ggtccattaa ggacatgtgt catatcgata actaataagc cttcaaacgg ccctttgctt
     5821 tcattatttg tcatttgctt atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca
     5881 tttatgtaaa atcaataaca tgattaatta tgaaaccagt cgactgacat aaatccacaa
     5941 gggagccgtg acgacaaagc ccagtacgct cacagccaat gacgctgtac cagtacgggt
     6001 gtagacatta aaccggctgg caataatgat tccggagaat gccggcggta atgcgcctgc
     6061 cagtaccatc atctgcaaat gttcgctgtt caaatgacat gccataccaa cgagaagcag
     6121 cgccagtggc atcagaatca acttcaggaa ggtgttataa gcaatttcgg cactgaactc
     6181 gaatttatgt gcagccagag tcaaccccgc agcgaatacc gctacccctg agttagcctt
     6241 cgcaatcaga ttaaaggttg ggtcccatgc tgccggaatt tttaccccaa ccagcaccag
     6301 gatcgttgcc agaacaggtg cccatactac tggctccttt gccgcagaaa ttaatgcgct
     6361 cagattacta ttcttcttac catccgctcc tgaggaagga ttcagcaaat acagaccaat
     6421 aggaatagta attgcgttaa caataataga aataattgct accactaaac ctgttgatac
     6481 ggaatcacca taaataggat cgagaactgc aaaccccagg aatccaatgg taggtgaacc
     6541 cgcaattaat gcacatacag ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaacc
     6601 aaaccaggag aaaaagaaac atccgacaat aaccacaagt gatacaaggg tcagacgagt
     6661 gtccgcaaaa atcatttccc tgtttgcccg ggtaatagaa acaaatagag ccgcaggaag
     6721 agcgtagttt aataccagtt tattaaatgc gcgggcttga tcttctgaaa aagtttctcg
     6781 tctgccgcta aaataaccca acagcatgat tacgataata ggcaataaat cgccaataaa
     6841 aaatgttagc ataatagtat ttccgataag ggtcccctca ccatcgcata atgagttggc
     6901 aagaggagac gatgtcagta tttaattacc agcgacttgt tttgggttaa gtttggtaat
     6961 atggccactt tcagttcctg ctgcagggtc gataaccaca ttaataatgg tcggtttgcg
     7021 cgactgaata ccggtggtta aagcatgacg aagttcatct gtcgtggtga cgttatagcc
     7081 aacgccacga aacgcatcca ttaatttgtc ataccttgca tggtgcaaca gatccgttgg
     7141 tgatggtgca ccagcgccac tgagatcaac accgtctcct ctgtagatgc cgccattatt
     7201 aaaaataacg atcgtcaccg gcaggttata tcgacaaatc gtttcaattt ccatcccact
     7261 gaaaccaaaa gcactatcac cttcaatggc gacaaccgga gaaccagaag tcacgctagc
     7321 accgatggca tagcccatac cgatgcccat gacaccccag gtaccacaat ccagacgacg
     7381 acgtggttta tacatatcaa taatatttcg tgcattatcc agggtatttg caccttcatt
     7441 aactaaataa atatcctggt tctcgcgcaa tacgtcgcgc acagcactta atgcattaaa
     7501 gtaatttaat ggttgagtat ctgtacttaa tttttcatgc attttttgtg cattttgctg
     7561 cttgtggata tttaaaatat cgcgccatac cagtggagtc gtaaatgtgt tttgtttcag
     7621 ttctgccagc ataccttgca tactggatgc aatatcacca acgactggca cagcaatggg
     7681 gcggttgctg tcaatttcct gcggttcaat atccagttga ataaactgtg tatctgccgc
     7741 ccatcctttt ttaccgtgtg ccaataacca attcagtcgt gcaccaacaa gcatgacaac
     7801 gtcagcattt gccagggcaa acgaacgcgc agctgccgca gaaagtggat gcgtatcttc
     7861 aaggatccct ttcgccatag acattggcag gaatggaatc tgagcacttt caataaattc
     7921 acgaagctgt tcatcagctt gtgaatacgc cgcgcctttg ccaaggataa ttaatggccg
     7981 ttcagctttt gctaaaagcg aaattgcgct agtgactgac ttcgggcatg gcaataatgc
     8041 tggcgacgga ttttcaactt taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc
     8101 ggccaggaca tttgctggca aatcaagata aactccgcca gggcgacccg atacagagac
     8161 ccggatagcg cgtgccaatg caatgccaag atcctgcggc tgattaacgc gaaatgctgc
     8221 tttggcatac ggttttgccg cattcatttg gtccagctct tcataatcac cttgctgtag
     8281 gtcgacgatc gcgcggtcgc tggagccgct aatcatgata atcggaaaac catttaccgt
     8341 tgcgttggcc aatgcggtca aaccattgag gaatcctggc gcagaaactg tcaggcagat
     8401 ccccggtttt tgggtaagaa aaccgcttgc cgcagcggca tagcctgccg actgctcatg
     8461 acgaaaacca atataacgaa tgccttccgc ctgggcatgg cgtgccatat ccgtcacagg
     8521 aatacctaca acaccataaa tagtgtcaat attattctgt tttaatgctt caacgatgat
     8581 atgcatacca tctgtcattt gaagttgatc tgacatgatt aataacctcg aaatttttat
     8641 aaattgggag ccagaagccc ccgttaatat cagatggcgt ggttttgctt cattgcagca
     8701 atttcatcgt cgctataacc cagctcctgc aatacagcag cggtatgttc acctaatagc
     8761 ggcgcagctt taatatccgg cgtaaaggca gagaatttca ttggacaacc aacggtcaga
     8821 tattttccac gcaacggttg ttccacttca acaacactgc cactttggcg caaagaggga
     8881 tcaagtgaaa tttctttcat acttaaaacc ggtgcacaag gaatatcaaa ctgagtcaaa
     8941 taggccaccg cttcatgttt atcaatagtg acagtgtatt tttcgatttc agcaaaaata
     9001 tcgaaaatat gtggctgtcg ggcatgggct gtactgtatg ccggatcggt aatccattct
     9061 ggtttgccga tggctttaca ggtgttttcc cagttttgct cctgaatagt gaaataaata
     9121 taggcgttag gatcggtttc ccagccttta catttcagga tccagccagg ctgaccgcca
     9181 ccacctgcat taccaccgcg gggaactgca tcaccaaatg taccattcgg atactgcggg
     9241 tattcttcca gataacccaa tttatcgaga cgctgctggt cacgtaattt cacgcggcaa
     9301 aggttcaata cggcatcctg cattgacatg gtgactcgtt gcccacgccc cgttttttcg
     9361 cgatgcagca aagcagcaag taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc
     9421 aacgctgcag cgcttaccag cggcggacca tcccaaaaac ccgtagtgga tgccgcgcca
     9481 cccgctgcct gagcaacgtt ttcataggct tttacattca cataaggcga acactcatca
     9541 aaccctttga tcgaaccaaa aatcagacgt ggattgattt cttgaatatg ctcccaggtg
     9601 aagcccatgt gatcaatggc ccctggatga aagttctcga ctaagatatc agcttcgcgg
     9661 atcagctttt ccattacctc tttgccttcc gctgttttgg tatttaactc aatagaacgt
     9721 ttgttactgt taagcatggt gaagtaaagc gcatcgatat caggaatatc tcgcagttgg
     9781 tgacgcgtta cgtcaccaac gccgggacgt tcaattttaa taacgtcagc gccaaaccag
     9841 gccagcattt gagtacaaga tgggccagat tgcacaccgg tgaaatcgag aactttaatt
     9901 ccttgaagtg gagttgacat agcaatacct attagtt
//
LOCUS       DAZPDE010000137         9906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPDE010000137 DAZPDE010000000
VERSION     DAZPDE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.137"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..78
                     /locus_tag="ACHU0J_003082"
     CDS             <1..78
                     /locus_tag="ACHU0J_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217666.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="ACHU0J_003083"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="ACHU0J_003083"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1217667.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="ACHU0J_003084"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="ACHU0J_003084"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1217668.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="ACHU0J_003085"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="ACHU0J_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1217669.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /gene="ycaO"
                     /locus_tag="ACHU0J_003086"
     CDS             complement(4692..6452)
                     /gene="ycaO"
                     /locus_tag="ACHU0J_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1217670.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="ACHU0J_003087"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="ACHU0J_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="ACHU0J_003088"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="ACHU0J_003088"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1217671.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="ACHU0J_003089"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="ACHU0J_003089"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1217672.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2483 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatgtgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattgt
//
LOCUS       DAZPDE010000138         9861 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPDE010000138 DAZPDE010000000
VERSION     DAZPDE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.138"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(114..458)
                     /locus_tag="ACHU0J_003090"
     CDS             complement(114..458)
                     /locus_tag="ACHU0J_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1217673.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            590..2500
                     /locus_tag="ACHU0J_003091"
     CDS             590..2500
                     /locus_tag="ACHU0J_003091"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1217674.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2558..3253
                     /gene="tsaB"
                     /locus_tag="ACHU0J_003092"
     CDS             2558..3253
                     /gene="tsaB"
                     /locus_tag="ACHU0J_003092"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1217675.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3293..3874
                     /gene="yeaY"
                     /locus_tag="ACHU0J_003093"
     CDS             3293..3874
                     /gene="yeaY"
                     /locus_tag="ACHU0J_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1217676.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4080..5765
                     /gene="fadD"
                     /locus_tag="ACHU0J_003094"
     CDS             4080..5765
                     /gene="fadD"
                     /locus_tag="ACHU0J_003094"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1217677.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5847..6962
                     /gene="rnd"
                     /locus_tag="ACHU0J_003095"
     CDS             5847..6962
                     /gene="rnd"
                     /locus_tag="ACHU0J_003095"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1217678.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7016..7981)
                     /gene="yeaX"
                     /locus_tag="ACHU0J_003096"
     CDS             complement(7016..7981)
                     /gene="yeaX"
                     /locus_tag="ACHU0J_003096"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1217679.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8037..8654)
                     /gene="yeaW"
                     /locus_tag="ACHU0J_003097"
                     /pseudo
     CDS             complement(8037..8654)
                     /gene="yeaW"
                     /locus_tag="ACHU0J_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(8996..9604)
                     /locus_tag="ACHU0J_003098"
     CDS             complement(8996..9604)
                     /locus_tag="ACHU0J_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1217680.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9774..>9861
                     /locus_tag="ACHU0J_003099"
     CDS             9774..>9861
                     /locus_tag="ACHU0J_003099"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1217681.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2394 a         2417 c         2590 g         2460 t
ORIGIN      
        1 ataatgaatt agcagaagca tcaacagtaa tatcttattc tctggtcgat ttatgtaaaa
       61 ccgaccagta ttatcgataa attatcgtct gacttatttt gcttcgataa agcttacacc
      121 gcagccacaa tcttaatttc aactttatac ttcgggttca tcaaacccgc ttgtaccgtg
      181 cagcgcaccg gcgcatgacc cgcgacaacc caggcatccc acgctttatt catcgccgcg
      241 aagtcgtttt tatcggccag gaaaatggtg gcatccagaa tgctcgattt attgctgccc
      301 tgtttttcca gcacggcgtc aatctgtgcc agcgtgttgg cggtttgctc aaaggcatcg
      361 gcgtcgaggt tttccggtac accagtgtag tagagcgtgt tgttgtggat tactacatca
      421 gaccagcggg cttcagcatc gatacgaacg atagtcatca ttttgtcctc attatacttc
      481 cattttcgtg ctgcaaagac tgccacaatg tgaggttgcc gtcaccactt caactggcga
      541 aagcgccctc atcctgacat aatccctgtt caaatcaaca gggggtagtg tgacggacga
      601 ttttgcacca gacggtcagc tggcgaaagc gataccaggc tttaagccgc gagaaccaca
      661 gcgacagatg gcggtagccg tcacccaggc gatagaaaaa ggccagccgc tggtggtgga
      721 agcaggaacc ggtacgggca aaacctacgc ttacctggct cctgcgctgc gggcgaaaaa
      781 gaaagtcatt atctcgaccg gctcaaaagc gttgcaggat cagctctaca gccgcgattt
      841 gccaacagtc tcaaaggcat tgaaatatac gggcaacgtg gcgctgctga aagggcgctc
      901 aaactacctc tgcctcgaac gtctcgaaca gcaggcgctg gcggggggcg atctgccggt
      961 acaaatctta agcgatgtga tcctgctgcg ctcctggtct aatcaaacag tcgatggtga
     1021 tatcagcacc tgcgtcagcg tggcggaaga ttcacaggcg tggccgctgg tcaccagcac
     1081 caacgacaac tgtcttggca gcgactgccc gatgtataaa gattgctttg tggtcaaagc
     1141 acgtaaaaaa gcgatggacg ccgatgtggt ggtggtaaac catcatctct ttctggcgga
     1201 tatggtggtt aaagagagtg gatttggcga gttgatccct gaagcggacg tcatgatctt
     1261 cgacgaagcc caccagctac cggacattgc cagccagtat tttggtcagt cactctccag
     1321 tcgacaactg ctcgacctgg caaaagacat caccatcgcc taccgcaccg aattaaaaga
     1381 cacccagcag ttacaaaagt gcgctgatcg tcttgcccag agtgcgcagg attttcgtct
     1441 gcaactcggt gagccaggtt atcgcggtaa cctgcgtgag ctgttagcta atccgcaaat
     1501 tcagcgggca tttttactgc tcgatgacac cttggaactt tgttatgatg tggcgaaact
     1561 gtcactgggg cgttccgcct tgctggatgc ggcatttgag cgcgccacgt tgtatcgcac
     1621 acggctgaag cggctaaaag agatcaatca gccgggctac agctactggt acgaatgcac
     1681 ttcgcgccat tttactctgg ctctcacgcc gctcagcgtg gcggataaat tcaaagagtt
     1741 aatggcgcaa aagcccggta gctggatctt cacctcagca acgctgtcgg tgaacgacga
     1801 tctgcatcat ttcacctcgc ggcttgggat agaacaggcg gagtcgttgc tattgccaag
     1861 cccgtttgat tacagccgcc aggcgttact ctgtgtgccg cgcaatctgc cgcaaaccaa
     1921 ccagccaggt tctgctcgcc agttagcggc aatgctgcga ccgatcatcg aagctaacaa
     1981 cggtcgttgt tttatgcttt gtacctcgca cgccatgatg cgcgatctgg ccgagcagtt
     2041 ccgcgctacc atgacgcttc ccgttttgtt gcagggggaa accagtaaag gccaactgtt
     2101 gcagcagttt gtcagtgccg gtaatgcgct tcttgtggca accagtagtt tctgggaagg
     2161 ggtggacgtg cgtggcgata cattgtcatt ggtaattatc gacaaattgc cgtttacctc
     2221 gccggatgat ccactgttaa aagcgcgcat ggaagattgt cgtttgcgcg gtggcgaccc
     2281 gttcgatgaa gtgcaactac cagatgccgt cattactctc aaacaggggg taggtcgact
     2341 gattcgcgac gccgacgatc gtggcgtgct ggtgatttgt gactatcggc tggtgatgcg
     2401 tccttacggc gcgacgtttc tcgccagtct gccgcccgcg ccacgcaccc gtgacattgc
     2461 ccgtgcggtt cgtttccttg cgataccatc ctccaggtaa tttgttacgg agtatggtat
     2521 gatgcgcgcc tttttattga tctaagcacg aagacccatg cgaattctgg ctatcgatac
     2581 cgcgacagag gcctgctctg tcgccctgtg gaacgacggt actgtcaacg ctcattttga
     2641 gctttgccct cgtgaacata ctcaacgaat cttaccgatg gtgcaggata tcctgaccac
     2701 cagcggaact tccctgactg atattaacgc tctggcttac gggcgcggcc ccggtagctt
     2761 taccggcgtg cgcattggta ttggcatcgc gcaagggctg gcgcttggcg cggaattacc
     2821 gatgattggc gtctccacgc taatgacgat ggcgcaaggg gcgtggcgca aaaacggcgc
     2881 aacacgcgtg ctgtcagcca ttgatgcgcg aatgggcgaa gtttactggg ccgaatatca
     2941 gcgtgatgaa aacggtatct ggcacggtga agaaaccgaa gccgtactca aacccgaact
     3001 cgttcatgaa cgaatgcaac agctttccgg cgaatgggtg acggtaggga cgggctggca
     3061 agcctggccg gatctcggta aagagagcgg gctggttttg cgcgatggcg aagtgttact
     3121 ccccgctgca gaagatatgc tgccgattgc ttgtcagatg tttgccgagg gtaaaacggt
     3181 ggcggtggaa catgccgagc cggtttattt gcgtaacaac gtcgcatgga agaaacttcc
     3241 gggcaaagaa tgaatcttaa gagtatgccc tgagaaaaaa aggagtcgca tcatggcggt
     3301 tcaaaagaat gttatcaaag gcatactggc aggtacgttt gcgctaatgc tgagcggttg
     3361 tgtcactgtg ccggacgcca ttaaaggcag cagtaccacg ccgcaacaag atttagttcg
     3421 ggtgatgagt gcgccgcagc tgtacgttgg tcaggaggca cgctttggtg gcaaagtggt
     3481 tgcggtacaa aaccagcaag ggaaaacccg cctggaaatt gctaccgtac cgctggacag
     3541 cggagctagg ccgacgctgg gagaaccttc tcgcggtcgc atttatgccg atgtgaacgg
     3601 ttttctggac ccggtggatt tccgtggaca actggttacg gtagtcgggc caatcaccgg
     3661 tgcggttgac ggcaaaatcg gcaatacgcc ctataaattt atggtgatgc aagtaacggg
     3721 ttacaaacgt tggcatttaa cccagcaggt gattatgccg cctcagccga ttgatccatg
     3781 gttttatggc ggtcgtggct ggccctatgg ctacggcgga tggggctggt ataatcccgg
     3841 ccccgcgaga gtacaaacag ttgtaaccga ataattgctt gtttttaaag aatgcagaaa
     3901 cagcggctgg tccgctgttt ctgcattctt acggtaaaga taaaaataaa tagtgacgcg
     3961 cttcgcaacc ttttcgttgg gtaattatca agctggtatg atgagttaat attatgttaa
     4021 cggcgtgtat atcatttggg gttgcgatga cgacgaacac gcattttaga ggtgaagaat
     4081 tgaagaaggt ttggcttaac cgttatcccg cggacgttcc gacggagatc aaccctgacc
     4141 gttatcaatc tctggtagat atgtttgagc agtcggtcgc gcgctacgcc gatcagcctg
     4201 cgtttgtgaa tatgggggag gtgatgacct tccgcaagct ggaagaacgc agtcgcgcgt
     4261 ttgccgctta tttgcaacaa gggttggggc tgaagaaagg cgatcgcgtt gcgttgatga
     4321 tgcctaactt attgcaatat ccggtggcgc tatttggcat tttgcgtgcc gggataatcg
     4381 tcgtaaacgt taacccgttg tataccccgc gtgagcttga gcatcagctt aacgatagcg
     4441 gcgcatcggc gattgttatc gtgtctaact ttgcccacac tctggaaaaa gtggttgata
     4501 aaaccgccgt tcagcacgta attctgaccc gtatgggcga tcagctatct acggcaaaag
     4561 gcacggtagt caatttcgtt gttaaataca tcaagcgctt ggtgccgaaa taccatctgc
     4621 cagatgccat ttcatttcgt agcgcactgc ataacggcta ccggatgcag tacgtcaaac
     4681 ccgaactggt gccggaagat ttagccttcc tgcaatacac cggcggcacc acaggtgtgg
     4741 cgaaaggcgc gatgctgact caccgcaata tgctggcgaa cctggaacag gtcaacgcca
     4801 cctatgggcc gttgctgcat ccaggtaaag aactggtagt aacggcactg ccgctgtatc
     4861 acatttttgc cctgaccatt aactgcctgc tgtttatcga actgggtggg cagaacctgc
     4921 ttatcactaa cccgcgtgat attccagggt tggtaaaaga gttagcgaaa tatccgttta
     4981 ccgctatcac tggcgttaac accttgttca atgcgttgct gaacaataaa gagttccagc
     5041 agctggattt ctccagtctg catctttccg caggcggtgg gatgcctgtt cagcaagtgg
     5101 tggcagaacg ctgggtgaaa ctgaccggac agtatctgct ggaaggttat ggcctgaccg
     5161 agtgcgcgcc gctggtgagc gttaacccgt atgatattga ttatcatagc ggtagcatcg
     5221 gtttgccggt gccatcgacg gaagccaaac tggtggatga cgacgacaac gaagtaccac
     5281 cgggtcaacc gggtgagctt tgtgtcaaag gaccgcaggt gatgctgggt tactggcagc
     5341 gtccggatgc tacagatgag atcatcaaaa atggctggtt acacaccggc gacatcgcgg
     5401 tgatggatga agaaggattc ctgcgcattg tcgatcgtaa aaaagacatg attttggttt
     5461 ccggttttaa cgtttatccc aacgagattg aagatgtcgt catgcagcat cctggcgtac
     5521 aggaagtcgc ggctgttggc gtaccttccg gctccagtgg tgaagcggtg aaaatcttcg
     5581 tagtgaaaaa agatccatcg cttaccgaag agtcactggt gactttttgc cgccgtcagc
     5641 tcacgggata caaagtaccg aagctggtgg agtttcgtga tgagttaccg aaatctaacg
     5701 tcggaaaaat tttgcgacga gaattacgtg acgaagcgcg cggcaaagtg gacaataaag
     5761 cctgagcgtt aagtcagtcg tcagacgccg gttaatccgg cgtttttttt gacgcccact
     5821 aaagagaaaa caatttgaat taccaaatga ttaccacgga cgatgcgctg gcttctttgt
     5881 gtgaagccgt ccgtgtcttt ccggcgatag ccctggatac tgaatttgtt cgtacgcgca
     5941 cttattaccc gcagctgggg ttgattcaac ttttcgatgg cgagcatctg gcgctaattg
     6001 atcctctcgg gatcaccgac tggtcaccgc tgaaagcgat cctgcgcgat ccgtccatca
     6061 caaaatttct ccatgcaggc agtgaagatc tggaagtgtt cctcaatgtc tttggcgaat
     6121 taccacaacc cttgattgac acgcaaatcc ttgctgcctt ctgcggacgc ccgatgtcat
     6181 ggggtttcgc ttccatggtg gaagagtatt ccggcgttac gctggacaag agtgaatcgc
     6241 gcaccgactg gctggccaga ccgctgaccg aacgtcagtg tgaatacgca gcggcggatg
     6301 tctggtatct gttaccgatc accgccaagc ttatggtaga aacggaggcc tccggctggc
     6361 tacctgcggc gctggatgaa tgccgcctga tgcaaatgcg tcgtcaggaa gtcgttgcgc
     6421 cggaagatgc ctggcgtgat atcaccaatg cctggcagtt acgcacacgc caactggcct
     6481 gtctgcaact gttagccgac tggcgactgc gcaaggcgcg agagcgtgat ctggcggtga
     6541 actttgtcgt gcgtgaagag catttgtggt cggtcgcgcg ttatatgccg ggaagtttag
     6601 gcgaactgga cagcctgggt ttatccggta gcgaaatccg ctttcacggt aaaacgctgc
     6661 tggcgctggt ggaaaaagcg cagacattgc cggaagatgc cttaccgcag ccgatgctta
     6721 acctgatgga catgcctggt tatcgtaaag cgtttaaagc gattaagtcg ctgattactg
     6781 acgtgagcga aacgcataag atcagcgccg aattgctggc atcgcgtcgg caaatcaacc
     6841 aactgctgaa ctggcactgg aaactgaaac cgcagaacaa tttgccggag ctgatttccg
     6901 gctggcgtgg tgagctgatg gcggaagcat tacacaattt attgcaggaa tatccgcagt
     6961 aaaatcttcc gaagccggac tgggcgcgct cagcgccaca tccggctttg gcaaactaca
     7021 aatccaacac caggcgttta cccttcgcac gcgaacaaca gatcaacata ctttgctggc
     7081 tggcacgctc ttcatcgcta aaatactgat cccgatggtc agcttcacct tccagtattg
     7141 ccgtttcgca ggttccgcat accccttcac gacataaaca ttccactttc gcggctttat
     7201 tattttcaat aacctgcaaa atagtcatct cttccggcac cacaaactct tttccggaac
     7261 gggcaagcac cagggtaaat gcatcgccgg ttttgtcttc gatagcaaat tgctcaaagt
     7321 gcagtgtatc ggcggcgatg tccagacgcg cagcttcact tcttaccgct tcaattagcg
     7381 cctcggggcc acaggtataa acgtgtgtgc caggttcgat atccgccaat aatcgcgcta
     7441 attccagtcg tgttccggtt gatgaatgat gcaaatggac tttctcagcc tgcggatgct
     7501 ggactagctc agcacgaaat gcgcaacttt ctggatttcg cgagcagtaa tgtagctgcc
     7561 agtcgatgtc gctgtgttgc aactctgcca tgtgcgacag gaaaggggtg ataccaatac
     7621 cgcccacgat aaacagatgc tttctggctg agggaattag cgcaaacagg ttattaggcg
     7681 ttgagatcgt taaccgatcg cccactttta cctgctgatg caaaaagcgg gaaccgccgc
     7741 gcgagttttc ttccagtcga acggcaatct gataactaga ggtgttatgc ggtgaactca
     7801 gtagtgaata cgcattgctg tactggttat caccatcgct catctgcact aggatgtgac
     7861 ttcctccggt gaacgcaggc agcggtttgc catcggttgc caccagcgtg aagcgtttca
     7921 cctgttcggt aaggggttca acctggctca cctgtacttc aaacatttga tagtctgaca
     7981 ttgctgtact ccatcaaaaa tcagcccagc ggcggaaaat accgccgccg atgtcattag
     8041 tccttaaaca cctgcgccag cagattatgg aaatgggcga taccatgttc ggaaatgcca
     8101 ctaccgctac tgtcggccat gatgcgcccc tgaccacgat agccacgcga tttcagccct
     8161 ttctgtacgc tttcaaccag acgtaaatct tccggatgga acacatcgcg ataccactca
     8221 atcagcgatt tttgctcgtc ggttaactct tcattggtga agtaaatatc gtagttttgc
     8281 agggtagttt cagaatccac cgggaattca taaatgaccg tcatcatccc tttgatcggg
     8341 gtgacgttca gcatcgtgca cggccacagc cagaaaccgt ggaatgccgc atccgtaccc
     8401 tcttcaaatt taaacgactg ttcggacggt ttggcgaaac cgtattgcag cgtccagtta
     8461 ccgtgcatgg tgtgccagta acgatcaacc tgtacggagt cggagaaacc tagatgcgcc
     8521 ggaccacagt gatagcactc gagatagtta tcgacaatgt tcttccagtt ggcaggcgtg
     8581 cgggtggtaa agcgggccgc tagtttcaga ttgtggactt ccgggcaggc ttccagcact
     8641 ttcgcgccca gaccggtgta ttgattcact tgaagtacga aaaaaaaccg ggaggacatt
     8701 ggattattcg ggttctgatg ggattagatt tggtggggct tgcaagcctg tagcgcaaat
     8761 tttagtcgtt aatcaatgaa acgcgaaaga tagtaaaaaa ttgcttttgt ttcattgaaa
     8821 atacgaaaaa caaaaacact gcaaatcatt tcaataacag cttcaaaaaa cgttcaaaac
     8881 cgataacaac caggctgtaa ccaaatgact catatcacaa atcagctcat gccgtttagg
     8941 tatgttacat gtgtgattat gtgaggtgaa gtatgtttta gctggttcat ggttgttata
     9001 cggctttttt tacatcttgt ggttcttgtg aaggagctac aagaccttcc tgttcataaa
     9061 taccatactt tgacaaaatc tttttacgag tatctgttgg taatgattca agcattttgg
     9121 ctgtttgtaa ggtttgctgc gaaatacctt gcaaatcagc ctggatggaa tcatcggaag
     9181 aaaaaatctc aggcataaga gcttcaaatc ctttctttct gaacaactca atcgcagaat
     9241 gcagttcctt gtcctctaac aattcgacaa ttgcgatcag tttagttttc aatgtgctgc
     9301 ggtcaggttg agaattttta ttctcttgat tatttttatg ctcaaccatt ggcatgctct
     9361 gactttggat aaaccactct attggacgcc ctgttgcgta ggctatttgt gctaatcgat
     9421 ctaaagacgg gtatgtcttg ccagacaggt agtttctgat aaccccatca gaaagtccgc
     9481 acttagctgc aaacgcccgg cttgattctc cattcattgc ctcttcaagg cgttccttga
     9541 aacgcattat ttccgggggc ataaaaatgc gctctgcatt tttgctttct ttttcacctg
     9601 tcatgcccaa tctctctgtt atataaagaa ttttacagaa ttctttgatt tgcactggtt
     9661 aaaataaaaa tgcgcatagc ttttgaattg agcatttttc tgcgctatcg tttttcccga
     9721 tagcggtagc cggtatcagt taccgaaaac gttaagagag taaggatcgc agaatgtcag
     9781 caaaaaacca agactggcac gcagagcaga taaaggctgc tgttcgtatg aaggggaaga
     9841 cactatctca attatccaga g
//
LOCUS       DAZPDE010000139         9826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPDE010000139 DAZPDE010000000
VERSION     DAZPDE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.139"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHU0J_003100"
     CDS             383..1501
                     /locus_tag="ACHU0J_003100"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1217682.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHU0J_003101"
     CDS             1567..1815
                     /locus_tag="ACHU0J_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR1217683.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHU0J_003102"
     CDS             1880..2248
                     /locus_tag="ACHU0J_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1217684.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHU0J_003103"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHU0J_003103"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1217685.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHU0J_003104"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHU0J_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1217686.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHU0J_003105"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHU0J_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1217687.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKAPLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHU0J_003106"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHU0J_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1217688.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHU0J_003107"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHU0J_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2406 a         2721 c         2379 g         2320 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cggcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatac
//
LOCUS       DAZPDE010000140         9774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPDE010000140 DAZPDE010000000
VERSION     DAZPDE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.140"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHU0J_003108"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHU0J_003108"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1217689.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHU0J_003109"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHU0J_003109"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1217690.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHU0J_003110"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHU0J_003110"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1217691.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHU0J_003111"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHU0J_003111"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1217692.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHU0J_003112"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHU0J_003112"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1217693.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHU0J_003113"
     CDS             complement(6031..6360)
                     /locus_tag="ACHU0J_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1217694.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHU0J_003114"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHU0J_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1217695.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHU0J_003115"
     CDS             7998..8171
                     /locus_tag="ACHU0J_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1217696.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHU0J_003116"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHU0J_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1217697.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZPDE010000141         9680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPDE010000141 DAZPDE010000000
VERSION     DAZPDE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.142"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHU0J_003117"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHU0J_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHU0J_003118"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHU0J_003118"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1217698.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHU0J_003119"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHU0J_003119"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1217699.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHU0J_003120"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHU0J_003120"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1217700.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHU0J_003121"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHU0J_003121"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1217701.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHU0J_003122"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHU0J_003122"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1217702.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHU0J_003123"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHU0J_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHU0J_003124"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHU0J_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1217703.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHU0J_003125"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHU0J_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1217704.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHU0J_003126"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHU0J_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1217705.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHU0J_003127"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHU0J_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1217706.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHU0J_003128"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHU0J_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1217707.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHU0J_003129"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHU0J_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1217708.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2479 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt
//
LOCUS       DAZPDE010000142         9572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPDE010000142 DAZPDE010000000
VERSION     DAZPDE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.143"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..133
                     /locus_tag="ACHU0J_003130"
     CDS             <1..133
                     /locus_tag="ACHU0J_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1217709.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="ACHU0J_003131"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="ACHU0J_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1217710.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="ACHU0J_003132"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="ACHU0J_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1217711.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHU0J_003133"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACHU0J_003133"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1217712.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHU0J_003134"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACHU0J_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1217713.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="ACHU0J_003135"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="ACHU0J_003135"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1217714.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHU0J_003136"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACHU0J_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1217715.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="ACHU0J_003137"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="ACHU0J_003137"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1217716.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZPDE010000143         9535 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPDE010000143 DAZPDE010000000
VERSION     DAZPDE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.144"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACHU0J_003138"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACHU0J_003138"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1217717.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACHU0J_003139"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACHU0J_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1217718.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACHU0J_003140"
                     /pseudo
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACHU0J_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4129..4884
                     /gene="actS"
                     /locus_tag="ACHU0J_003141"
     CDS             4129..4884
                     /gene="actS"
                     /locus_tag="ACHU0J_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1217719.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="ACHU0J_003142"
     tRNA            4963..5036
                     /locus_tag="ACHU0J_003142"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="ACHU0J_003143"
     CDS             5171..6781
                     /locus_tag="ACHU0J_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1217720.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6813..7232
                     /locus_tag="ACHU0J_003144"
     CDS             6813..7232
                     /locus_tag="ACHU0J_003144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217721.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8351..9097
                     /locus_tag="ACHU0J_003145"
     CDS             8351..9097
                     /locus_tag="ACHU0J_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1217722.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9395..>9535
                     /locus_tag="ACHU0J_003146"
     CDS             9395..>9535
                     /locus_tag="ACHU0J_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217723.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2421 g         2320 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta
     7621 agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc
     7681 tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc
     7741 catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt
     7801 tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat
     7861 cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa
     7921 ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt
     7981 cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat
     8041 ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta
     8101 gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact
     8161 taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc
     8221 agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc
     8281 cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga
     8341 gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat
     8401 gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat
     8461 tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag
     8521 cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg
     8581 tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta
     8641 ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct
     8701 tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac
     8761 ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct
     8821 ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa
     8881 gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta
     8941 taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa
     9001 gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct
     9061 gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac
     9121 ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc
     9181 ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc
     9241 aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt
     9301 taaacacctt ttgagatgac cgatgcgggg agacccgtag gccttactgt gcctgcgata
     9361 ccgatagtct caactctcaa gaggagaatt acctatgatg ttcaacaaca acaactggaa
     9421 gctgtcagtc accgacatca acctttatga aaacactgtg agtctggacg gtcagtccta
     9481 tccgttgtcg ctcgctatca agaccctcat tccgggctac ctgtcaggct taccg
//
LOCUS       DAZPDE010000144         9498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPDE010000144 DAZPDE010000000
VERSION     DAZPDE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.145"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHU0J_003147"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHU0J_003147"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1217724.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHU0J_003148"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHU0J_003148"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR1217725.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHU0J_003149"
     CDS             3115..3399
                     /locus_tag="ACHU0J_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1217726.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHU0J_003150"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHU0J_003150"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1217727.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHU0J_003151"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHU0J_003151"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1217728.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHU0J_003152"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHU0J_003152"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1217729.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHU0J_003153"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHU0J_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1217730.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2277 a         2501 c         2394 g         2326 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggctctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcga ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatt
//
LOCUS       DAZPDE010000145         9440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPDE010000145 DAZPDE010000000
VERSION     DAZPDE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.146"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHU0J_003154"
     CDS             <1..1154
                     /locus_tag="ACHU0J_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217731.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHU0J_003155"
     CDS             1833..2051
                     /locus_tag="ACHU0J_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1217732.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHU0J_003156"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHU0J_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1217733.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHU0J_003157"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHU0J_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1217734.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHU0J_003158"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHU0J_003158"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1217735.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHU0J_003159"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHU0J_003159"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1217736.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHU0J_003160"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHU0J_003160"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1217737.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHU0J_003161"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHU0J_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1217738.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2314 c         2446 g         2369 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gtcgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAZPDE010000146         9360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPDE010000146 DAZPDE010000000
VERSION     DAZPDE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.147"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHU0J_003162"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHU0J_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHU0J_003163"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHU0J_003163"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1217739.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHU0J_003164"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACHU0J_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHU0J_003165"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACHU0J_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1217740.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHU0J_003166"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACHU0J_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1217741.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHU0J_003167"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACHU0J_003167"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1217742.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHU0J_003168"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACHU0J_003168"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1217743.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHU0J_003169"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACHU0J_003169"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1217744.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHU0J_003170"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACHU0J_003170"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1217745.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2267 a         2457 c         2458 g         2178 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
//
LOCUS       DAZPDE010000147         9353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPDE010000147 DAZPDE010000000
VERSION     DAZPDE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.148"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHU0J_003171"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHU0J_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1217746.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHU0J_003172"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHU0J_003172"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1217747.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHU0J_003173"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHU0J_003173"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1217748.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHU0J_003174"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHU0J_003174"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1217749.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHU0J_003175"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHU0J_003175"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1217750.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHU0J_003176"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHU0J_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1217751.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHU0J_003177"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHU0J_003177"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1217752.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHU0J_003178"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHU0J_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1217753.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHU0J_003179"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHU0J_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1217754.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHU0J_003180"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHU0J_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1217755.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2391 a         2343 c         2378 g         2241 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gct
//
LOCUS       DAZPDE010000148         9201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPDE010000148 DAZPDE010000000
VERSION     DAZPDE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.149"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(78..980)
                     /locus_tag="ACHU0J_003181"
     CDS             complement(78..980)
                     /locus_tag="ACHU0J_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR1217756.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1118..2359)
                     /gene="yihS"
                     /locus_tag="ACHU0J_003182"
     CDS             complement(1118..2359)
                     /gene="yihS"
                     /locus_tag="ACHU0J_003182"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR1217757.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2376..3254)
                     /gene="yihT"
                     /locus_tag="ACHU0J_003183"
     CDS             complement(2376..3254)
                     /gene="yihT"
                     /locus_tag="ACHU0J_003183"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR1217758.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3278..4174)
                     /gene="yihU"
                     /locus_tag="ACHU0J_003184"
     CDS             complement(3278..4174)
                     /gene="yihU"
                     /locus_tag="ACHU0J_003184"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1217759.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4342..5238
                     /gene="yihV"
                     /locus_tag="ACHU0J_003185"
     CDS             4342..5238
                     /gene="yihV"
                     /locus_tag="ACHU0J_003185"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR1217760.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5272..6057
                     /locus_tag="ACHU0J_003186"
     CDS             5272..6057
                     /locus_tag="ACHU0J_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1217761.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6156..6755
                     /gene="yihX"
                     /locus_tag="ACHU0J_003187"
     CDS             6156..6755
                     /gene="yihX"
                     /locus_tag="ACHU0J_003187"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1217762.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6749..7621
                     /locus_tag="ACHU0J_003188"
     CDS             6749..7621
                     /locus_tag="ACHU0J_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1217763.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7618..8055
                     /gene="dtd"
                     /locus_tag="ACHU0J_003189"
     CDS             7618..8055
                     /gene="dtd"
                     /locus_tag="ACHU0J_003189"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1217764.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8100..9041
                     /gene="fabY"
                     /locus_tag="ACHU0J_003190"
                     /gene_synonym="yiiD"
     CDS             8100..9041
                     /gene="fabY"
                     /locus_tag="ACHU0J_003190"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1217765.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2148 a         2324 c         2466 g         2263 t
ORIGIN      
        1 aataaattga agatacataa tgttgccttt agcgagtgaa atgttcttca atgattttga
       61 ctgctaatca atacaagtta ttttctctgt ccgtaaatat taaaaaacag gcggtgcggt
      121 tttcctggtt ccagtaataa taagtcgata cctgaattaa aggcgttagg cgggcaactc
      181 ataggttcaa ctgccagtcc ctgacgctgt aatttttcac cgctataaac ctgcacccag
      241 ggttgatcgg aacaaaggct taccgaaagc gcctgctgtg gatgggtaat ggtcatttcc
      301 cacaaatcat tagcggtttt aaaggtgtgg tcgatttttg tcgcggctat ttttttcgcc
      361 tgcgtgaaat ttaaatccag ctcatcaacg tggtgaagcg ttgtcggatt tacgtgttca
      421 tcgacggcat aaacctggtt tgccggaagc tggaacagat attcatcaac agatgttaag
      481 ttgcaggtta aataaggatg aatacccacg ccataaggcg ctgcgacagt gccgatattc
      541 tggcttgcga tctcaacgga aagacccgtg tgtgcgttga gcgaataaac aacttgtgaa
      601 gccagcataa acggatagcc gtaactgggc ggcagaaaag ctgtcagggt aacgctggtg
      661 gtggttaatt cactaatttg ccagtctcgc caggcaagca gcccgtgaat agcggctttt
      721 gaactatgtt cattaatggg taactgatat tcctgcccct gataacggta acagccgttg
      781 gcgatccggt tcggccacgg aataagcact ttacccagat gcgccagcgg catctcttcg
      841 ggtttgtgcg ggatgactaa atgacaaccc tgaaaggtta attcagccag accagcaccg
      901 acggtcacaa tggtcgcgtg ataatccccc gcggcaagtg aaacggtctg tccactgcaa
      961 tgcatattgg tgatttgcat acaaggcacc ttaattaacg acatacagcc tcccgtgagg
     1021 ctgatgtcaa acccaaaaac tgcaccggac ggtcccctcg cccctttggg gagagggtta
     1081 gggtgagggg aaaacccgca ccctattttc agatcagtta tttcgcatta atatccagca
     1141 gacccgctgc aaccgctgga gccatgccgg gcgctaacgg gatacgcggg atcaccaggc
     1201 aatgcagcag gtgataaata tcctgtttgc cgtcccagac tttggtgatg accttattgt
     1261 ccgcatccag ctcctgccac caggaaccat tttcatagtc catcaggtac ttaatgcagt
     1321 actcccacca tgtttgatac caggtttcat actggcgatc gccagtgacg gtgtagagcg
     1381 cgtagaccgt acccattgct tcgacgatag gccaacgtac acgttcgcgg accaccggtt
     1441 ttccttccca gtcaacggta taaacaatcc cgtccgctcc atcgggtgcc caggcatcgc
     1501 gcacggtggc gttaaacaga cctttggcat cttctagcag ccatgctggt ggttgttcgc
     1561 aacgggcttc cagggcagca tggatgtgca gcattaaacg gccccattcg atccagtggc
     1621 ctggtgtacc tccgaacgcg cggaagcgat gcgccgggtt atctttgttg taatccggca
     1681 gcggattcca ctgggtatcg aagtgttcgt taacgcgata atgattattt ctggcgacgt
     1741 cgtggataat cacggaagcc acgcgaatcg cgcgatccag ccattttttg tcgtgagtga
     1801 cgtcataaac aatcaagaaa gcttccaccg cgtgcatatt ggcattgccg ccgcggtact
     1861 cttcggtttt gctgaaggct tcgtcccagg actccaggca catctgctct tcttcgctcc
     1921 agaaatattt ctcgataatc tcaatggtgt aatcgagcag cttgcgcgct tccgggtgac
     1981 ccgttgtgac ggcgctggcg gcacccagca gagcaaagaa gtgttgatag ccctgtttgg
     2041 aggcatccac cacgccctcg tcattcacgc aggcatacca gccgccatat tttttatcac
     2101 gcagtgcgcc gttcatggct ttaataccgt gatcaaccaa cgcgtaagcg ccaggtcgac
     2161 ccatcgccgc agcaacggaa taaacgtgca acatacgagc tgtgatccac agatgggtgc
     2221 ccatctcttc tttgatttgc cctttattgc ctaaccagcc aaaaccagtc ggcactacgg
     2281 aatttttacc aaaatcaaag atgcggtcgg tttcctgttc cagccaacgg ttgtggctta
     2341 gggtgttaaa ccatttcatt ccttttatcc tcatcttagc ggcgtttagc catcatttcg
     2401 tcgacgatat cgccaagttg ttgtaatttc ggtgcgcaaa cgtcacggag catcagctcg
     2461 ttgtctggta aaccgacgac cgatgcccag actgcacggc ctgccaggaa tcccgatgcg
     2521 cccgccgtca ttgccacgcg tacggcacgc gggaacagtt tttcgtcgac actggaagag
     2581 aggatcaccc atggcatatt gatatggtca ttcagacgtt gtgaagcaca gagaagctct
     2641 tgttgcggac ctttgccata aagcggcatt tcaactttgt agaggtcagc gccactgtcg
     2701 cccagctctt tggcggcatc gatgatcgct tgttcgcgat cgaatttatc gccacgacgc
     2761 ggtgggcgga cgactggctc aatgatgctt accagaccgt gtgagtggca cagttcgttg
     2821 aactctttca ccatatccag acgttgctgc gcatcttcat cactacgcca cagcaccagc
     2881 agttttaagg ctttgccacc gtcttgtttg atctgtagcg gattgatttt gcggtcaata
     2941 accacgctat cgaccggaat accgttgcca ggaatgaact catcggcggc gacaatcatg
     3001 gcgcaacttt tggcaatcgc gttttgctca accacctggc ggtagcagaa ttgttgatct
     3061 accagaatcg ccgaggcata aggcgagagg gcctttgcag cgttaacttt gaaatcagtt
     3121 aaaacgctat cggctaccgg agcgggtgcc ccagccgcgg caaacatcat gcgcatggct
     3181 tcgcgctgat cgaccgccag catggcaaaa ccgcccgatg cgcgcgtaat gtcgttgatg
     3241 gtgtacttat tcattcattt attcctttat ccagtcgtta catttttact ttggcagtca
     3301 tcccggcact gacacggacc tgttccagaa tggcggacca gtcctggcga ccgcgacccg
     3361 ctgcgcgcgc ctggctataa acctcccgtg aggcggcccc cagcggcatt ggcacatgca
     3421 gctggttggc gacatcaagg gcgatgccaa gatccttatg ggcaagatcg atcatgaagg
     3481 cgggagaaag atctccgctg aggactttgt ttggccagga agtggtgaag tgacctttac
     3541 cggcggcggt accgctcatc actttgacgg caacatcgaa gggaagattc agggcttcgc
     3601 acaaaacggc ggcttctgcc gaaagcgcat tgagcgcgat gctcatgtag ttgttgatga
     3661 gcttaacgcg gatccccatg cccggaccgc cagcgttgat caactcactg cccatcgcca
     3721 tcaggatcgg tgtggcacgt tcaacttgtt cagcggtgcc gccagccagc agtaacagag
     3781 taccggtaat ggcatttgca gaagtacggc ctaccggaac atccatcatg ctgaagcctt
     3841 tggcttgcat atcggcaatc aatttatcgg tttgcagcgg atggatggtg gacatatcaa
     3901 tgaccagcgc atcggtagat aagccttcgc aaacgccgtt ttcaccgaac aacacgttgc
     3961 gcaccagatc gccattcggc aacatggtaa tgataaattc ggcatcttta gcggcctgcg
     4021 ccgggttggc ggcgggagtc gcacctttgt ctaccagatg ccgcacagct tcggcgttca
     4081 catcaaagac gcgaagttga tgcccttgct gcaataaatt gctcgccatt ggcgaaccca
     4141 tttgtcctaa accgataaac gcgattgctg ccataaccct ctcctgaata cagtgatgtc
     4201 actttttgtc atttatgaca tgctttgctt gtctgttttt gatcgaattt gtaatttatc
     4261 gtcaaaaaat tgacagccgt cactttttaa acaattggtg aaattaaaat gaacgcatcc
     4321 caaaatgttt aaggaatgac catgattcgt gttgcttgtg taggtataac cgtgatggat
     4381 cgcatctatt acgtggaagg gttaccgacg gagagcggta aatacgtggc gagaaattat
     4441 acggaagttg gtggcgggcc agcggcgacg gcagcggttg cggcggcaag gctgggggcg
     4501 caggtcgatt ttattggtcg cgtaggtgat gacgacgcgg gcaacagcct gctggcagag
     4561 ctggaatcct ggggcgttaa cacccgttac accaaacggt ataaccaggc gaaatcttcg
     4621 caatccgcca tcatggtgga taccaaaggc gagcggataa tcattaacta ccccagcccg
     4681 gatctgctgc ctgacgcaga gtggttggag gaaattgatt tctctcagtg ggatgttgtg
     4741 ctggcagatg tacgctggca cgacggcgct aaaaaagcct tcaccctggc ccgtcaggcg
     4801 ggtgtgatga ccgttctgga cggggacatt acgccgcagg atatcagtga gctggtggca
     4861 ttaagcgatc acgcggcctt ttcagaaccg ggtctggcgc gcttaacggg cgtgaaagag
     4921 atggccagtg cgctaaaaca ggcacaaacg ctcacaaatg gtcatgtcta tgtgacccag
     4981 ggtagcgcag gctgcgactg gctggaaaat ggtgggcgtc agcatcaacc ggccttcaaa
     5041 gttgatgtgg tagataccac aggtgcggga gatgtttttc acggcgcttt ggcggtggcg
     5101 ctggcaacaa gtggggattt agcggagtca gtccgcttcg ccagcggtgt agcggcgtta
     5161 aaatgcacac gtcccggtgg acgagccggg atccctgact gtgatcaaac ccgatctttt
     5221 ttgtcacttt ttgtataaaa tgccagggtg atggtttttc gaggaatttt catgagcctt
     5281 accgaactaa ccggtaaccc gcggcacgac caactcctca tgctgatcgc cgagcgtggg
     5341 tatatgaata ttgatgagct ggcaaatctg ctggatgtct ccacgcagac ggtccgccgg
     5401 gatattcgta aattaagcga gcaaggcctg attacgcgcc atcacggtgg cgcgggtcgg
     5461 gcttccagcg tcgttaatac ggcgttcgag cagcgtgagg tttcgcaaac cgagaaaaaa
     5521 aaagcgattg ccgaagcggt ggcagactat attcctgatg gttcaacaat atttatcacc
     5581 attggtacga ctgttgagca tgttgcccgg gcgttactta accataatca tttgcggata
     5641 atcaccaaca gcctgcgtgt ggcgcatatt ctttaccaca acccgcgctt tgaagtgatg
     5701 gtgcccggcg gtacgttgcg ctctcataat agcgggatca ttggcccttc agcggcgtcc
     5761 tttgtggctg attttcgtgc tgattatctg gtaacaagcg ttggggcgat tgagagcgat
     5821 ggcgcgttga tggagtttga tgtaaacgaa gctaacgtgg tgaaaacgat gatggcgcac
     5881 gcgagaaata ttctgctggt cgccgatcac actaagtatc atgcttcggc ggcggttgaa
     5941 attggtaacg tggcacaggt cactgcgctc tttaccgacg agctgccgcc cgctgcgcta
     6001 aaatcacgct tacaagacag ccaaattgaa atcattcttc cccaggaaga cgcgtagatt
     6061 tttgtgaccg ttaacctggc ttcatacctt gccacatagc caaacccatc ctttcccgct
     6121 acagttaatt tcttgtggcg cgaaaggagg caaaaatgct ctatatcttt gatttaggta
     6181 atgtgattgt cgatatcgac tttaaccgtg tgctgggagc ctggagcgat ttaacgcgta
     6241 ttccgctggc atcgcttaag aagagttttc atatggggga ggcgtttcat cagcatgagc
     6301 gtggggaaat tagcgacgaa gcgttcgcag aggcgctgtg tcatgagatg gctctaccgc
     6361 taagctacga gcagttctct cacggctggc aggcggtgtt tgttgcgctg cgcccggaag
     6421 tgatcgccat catgcataaa ctgcgtgagc aggggcatcg cgtggtggtg ctttccaata
     6481 ccaaccgcct gcataccacc ttctggccgg aagaataccc ggaaattcgt gatgctgctg
     6541 accatatcta tctgtcgcaa gatctgggga tgcgcaaacc tgaagcacga atttaccagc
     6601 atgttttgca ggcggaaggt ttttcaccca gcgatacggt ctttttcgac gataacgccg
     6661 ataatataga aggagctaat cagctaggca ttaccagtat tctggtgaaa gataaaacca
     6721 ccatcccgga ctatttcgcg aaggtgttat gctaaaaacc attcaggaca aagccaggca
     6781 ccgtacccgt ccactatggg cctggctaaa actactctgg caacgtattg atgaggacaa
     6841 catgacaacc ctggcaggta accttgccta tgtgtcgttg ctctcattag tgccgctggt
     6901 tgccgttgtt tttgcgcttt tcgccgcttt tcccatgttt tccgacgtca gcattcagtt
     6961 acgtcacttt atttttgcca actttctgcc tgctactggc gatgttattc agcggtatat
     7021 cgaacaattt gttgctaatt ccaacaagat gaccgccgtt ggggcgtgcg ggctgatcgt
     7081 caccgcgtta ttgttgatgt actccatcga tagcgcgttg aataccatct ggcgcagtaa
     7141 acgagcgcga ccaaaaattt actcgttcgc cgtgtactgg atgattttaa cgctggggcc
     7201 gctgctggca ggggccagtc tggcgatcag ttcctatttg ctctccctgc gctgggcgag
     7261 cgatctcaat actgtcatcg acaacgtgct gcgtattttt ccgttgctgt tgtcgtggat
     7321 ctccttctgg ttgctgtaca gcattgttcc taccatccgc gtacctaacc gcgacgcgat
     7381 tgtcggcgcg tttgtcgccg cactcctgtt cgaagcagga aagaaaggtt tcgcgcttta
     7441 tatcaccatg ttcccgtcat atcagctcat ttacggtgtg ctggcggtga tccccattct
     7501 ctttgtttgg gtctactgga catggtgtat cgtcttgctt ggcgcggaaa ttactgtcac
     7561 tctcggggaa taccgcaaac taaaacaagc agctgaacaa gaagaagacg acgaaccatg
     7621 attgcattaa ttcaacgcgt aacccgtgcc agcgtcaccg tggagggaga agtgacgggc
     7681 gaaattggcg cgggactttt ggtgttattg ggtgtcgaaa aggatgacga cgaacagaaa
     7741 gcaaaccgtc tgtgcgagcg tgtgctcggc taccgcatct ttagcgatgc cgaaggcaag
     7801 atgaatctca acgtgcaaca ggcgggcggc agtgtactgg tggtttccca gtttaccctc
     7861 gccgcagata ccgaacgggg gatgcgccca agtttctcca aaggcgcatc accggatcgc
     7921 gcagaggcgt tatatgacta tttcgtcgaa cgctgccgcc agcaagagat gaacacgcaa
     7981 acaggacgct tcgctgccga tatgcaggta tcgctggtca atgatggccc cgtgacattc
     8041 tggttgcagg tatgagccag cttccagggt tgtcacggga aacaagagag agtatcgcta
     8101 tgtatcacct tcaggttcca caaacagaag aagagttaga gcgttactat cactttcgct
     8161 gggaaatgtt gcgtaagccc ctgcatcaac caaaaggttc ggaacgcgac gcgtgggatg
     8221 cgatggcgca tcaccagatg gtcgtcgacg agcagggtaa tctggtggcg gtaggccgat
     8281 tgtatattaa tgccgacaat gaagcgtcca ttcgctttat ggtcgttcac ccggacgtgc
     8341 aggacaaagg gttaggaacg ctgatggcga tgaccctgga gtcggtggcg cgtcaggaag
     8401 gcgttaagcg cgtgacctgt agcgcccgtg aagacgcggt ggagtttttc gccaagctgg
     8461 ggtttgttaa tcagggagaa atcaccacgc caaccaccac gccgattcgc cattttttga
     8521 tgattaagcc cgtcgccact ctggatgata ttttgcatcg cggcgactgg tgcgcgcagc
     8581 tgcaacaggc gtggtacgaa catatcccgc ttagtgaaaa aatgggcgtg cgtattcaac
     8641 aatataccgg gcaaaaattt atcaccacca tgccggaaac cggcaatcag aatccgcacc
     8701 atacgctgtt tgccgggagt ttattctcgc tggcgacgct caccggttgg gggcttatct
     8761 ggctgatgct gcgtgaacgc cacctcggcg gaacgattat tctggcggat gcgcatatcc
     8821 gctacagcaa accgattagc ggtaaacccc atgcggtagc cgaccttggt gccttaagcg
     8881 gcgatctcga ccgtctggcg cgcggacgaa aagcacgggt gcagatgcag gtcgaaatct
     8941 ttggcgacga gacgccgggt gcagtgtttg aaggcacgta tatcgttctg cccgcgaagc
     9001 catttggccc gtatgaagag ggcgggaacg aagaagagta gcttatggag aggggcggtg
     9061 ctgcctctct cattcattcc ggtcatttac atcagtccct gactattccg gttatattga
     9121 ataagaactt tataaatttt tattcttcgt tggcaccata cgtgtcttgc tacatcactg
     9181 ttcaggagca tttaaatatt a
//
LOCUS       DAZPDE010000149         9165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPDE010000149 DAZPDE010000000
VERSION     DAZPDE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.150"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHU0J_003191"
     CDS             <1..923
                     /locus_tag="ACHU0J_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1217766.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHU0J_003192"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHU0J_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1217767.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHU0J_003193"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHU0J_003193"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1217768.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHU0J_003194"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHU0J_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1217769.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHU0J_003195"
     CDS             4446..4805
                     /locus_tag="ACHU0J_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1217770.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHU0J_003196"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHU0J_003196"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1217771.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHU0J_003197"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHU0J_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1217772.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHU0J_003198"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHU0J_003198"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1217773.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2217 c         2475 g         2380 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgtacaccc cgcgc
//
LOCUS       DAZPDE010000150         9154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPDE010000150 DAZPDE010000000
VERSION     DAZPDE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.151"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHU0J_003199"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHU0J_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1217774.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHU0J_003200"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHU0J_003200"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1217775.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHU0J_003201"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHU0J_003201"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1217776.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHU0J_003202"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHU0J_003202"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1217777.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHU0J_003203"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHU0J_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1217778.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHU0J_003204"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHU0J_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1217779.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHU0J_003205"
     CDS             6064..6606
                     /locus_tag="ACHU0J_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1217780.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHU0J_003206"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHU0J_003206"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1217781.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHU0J_003207"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHU0J_003207"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1217782.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHU0J_003208"
     CDS             8003..8260
                     /locus_tag="ACHU0J_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1217783.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHU0J_003209"
     CDS             complement(8236..8790)
                     /locus_tag="ACHU0J_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1217784.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2299 a         2220 c         2279 g         2356 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgcaactgct
     9121 ctttaacaat ttatcagaca atctgtgtgg gcac
//
LOCUS       DAZPDE010000151         8872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPDE010000151 DAZPDE010000000
VERSION     DAZPDE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.152"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHU0J_003210"
     CDS             <1..439
                     /locus_tag="ACHU0J_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1217785.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHU0J_003211"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHU0J_003211"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1217786.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHU0J_003212"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHU0J_003212"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1217787.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHU0J_003213"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHU0J_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1217788.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHU0J_003214"
     CDS             complement(4441..5148)
                     /locus_tag="ACHU0J_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR1217789.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHU0J_003215"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHU0J_003215"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1217790.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHU0J_003216"
     CDS             complement(6006..6209)
                     /locus_tag="ACHU0J_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1217791.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHU0J_003217"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHU0J_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..8769
                     /locus_tag="ACHU0J_003218"
                     /pseudo
     CDS             7873..8769
                     /locus_tag="ACHU0J_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            complement(8785..>8872)
                     /locus_tag="ACHU0J_003219"
     CDS             complement(8785..>8872)
                     /locus_tag="ACHU0J_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1217792.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2062 a         2416 c         2273 g         2121 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
     8761 gaacaattcg gtaatgactc caacttactg atagtgtttt atgttcagat aatgcccgat
     8821 gaccttgtca tgcagctcca ccgattttga gaacgacagt gacttccgta cc
//
LOCUS       DAZPDE010000152         8869 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPDE010000152 DAZPDE010000000
VERSION     DAZPDE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.153"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            140..643
                     /locus_tag="ACHU0J_003220"
     CDS             140..643
                     /locus_tag="ACHU0J_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1217793.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHU0J_003221"
     CDS             complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHU0J_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1217794.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHU0J_003222"
     CDS             complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHU0J_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1217795.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1438..1761
                     /locus_tag="ACHU0J_003223"
     CDS             1438..1761
                     /locus_tag="ACHU0J_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1217796.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1932..2429
                     /gene="ftnA"
                     /locus_tag="ACHU0J_003224"
     CDS             1932..2429
                     /gene="ftnA"
                     /locus_tag="ACHU0J_003224"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1217797.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2466..2705)
                     /locus_tag="ACHU0J_003225"
     CDS             complement(2466..2705)
                     /locus_tag="ACHU0J_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1217798.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2895..4106
                     /gene="tyrP"
                     /locus_tag="ACHU0J_003226"
     CDS             2895..4106
                     /gene="tyrP"
                     /locus_tag="ACHU0J_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1217799.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4168..4833)
                     /gene="yecA"
                     /locus_tag="ACHU0J_003227"
     CDS             complement(4168..4833)
                     /gene="yecA"
                     /locus_tag="ACHU0J_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1217800.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5029..5115)
                     /locus_tag="ACHU0J_003228"
     tRNA            complement(5029..5115)
                     /locus_tag="ACHU0J_003228"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5079..5081),aa:Leu,seq:taa)
     gene            complement(5128..5201)
                     /locus_tag="ACHU0J_003229"
     tRNA            complement(5128..5201)
                     /locus_tag="ACHU0J_003229"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5167..5169),aa:Cys,seq:gca)
     gene            complement(5256..5331)
                     /locus_tag="ACHU0J_003230"
     tRNA            complement(5256..5331)
                     /locus_tag="ACHU0J_003230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5296..5298),aa:Gly,seq:gcc)
     gene            complement(5483..6031)
                     /gene="pgsA"
                     /locus_tag="ACHU0J_003231"
     CDS             complement(5483..6031)
                     /gene="pgsA"
                     /locus_tag="ACHU0J_003231"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1217801.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6088..7920)
                     /gene="uvrC"
                     /locus_tag="ACHU0J_003232"
     CDS             complement(6088..7920)
                     /gene="uvrC"
                     /locus_tag="ACHU0J_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1217802.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7917..8573)
                     /gene="uvrY"
                     /locus_tag="ACHU0J_003233"
     CDS             complement(7917..8573)
                     /gene="uvrY"
                     /locus_tag="ACHU0J_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1217803.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8715..8786
                     /locus_tag="ACHU0J_003234"
                     /pseudo
     CDS             8715..8786
                     /locus_tag="ACHU0J_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         2203 a         2248 c         2052 g         2366 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttccaggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tcaagcactc tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gcctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa
      661 tacattgcgc cgatgaagtt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt
      721 ttttaatggg attcaccctg tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg
      781 attgcccgca tggtgaccgg gtctgcggca gcgggatgat gagtaaagtc gatattcttc
      841 agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga
      901 atttgtttga taaccaactg gcaagctacc acatcagaaa ccagttcctg atcggcattc
      961 agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc
     1021 acatcttcag cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag
     1081 gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta
     1141 ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata
     1201 caggtgttat tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt
     1261 gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc
     1321 cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     1381 ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg
     1441 agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccag
     1501 gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct
     1561 tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc
     1621 cagcaggaag gctataccca cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt
     1681 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     1741 gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa
     1801 atagcggtaa tgcgttttat tcccattcgt atttttaata attgaagttt atattttacc
     1861 ttttgcaaat aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt
     1921 ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg
     1981 aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg
     2041 aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc
     2101 tgtttgatta cctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt
     2161 ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa
     2221 tcacccagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat
     2281 ttaatttcct gcagtggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga
     2341 ttattgataa attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag
     2401 aactctctac cctcgacaca caaaactaat gctctccggc ggcaggtttc tgccgccgtt
     2461 tcaaatcagt gacggcaaat cttactctga tcggtcgaaa aaccttcttc tgaaggtata
     2521 aatttgcctt ttgctgccag aaacgctacc agctctcccg ctgtcacccc ttctgccgag
     2581 cacgtgtgaa aacgtgcctg ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca
     2641 tgcgtatatt gctcgcctga ttcaatcatc atatttaaca cttcatgacc gtgaatagag
     2701 tccatcgttc ctcctcaaaa aaagcctagc gtagcgatta ccgcttatga agactttgcg
     2761 ccagcgcagg actgaatgct tttattgtac atttatattt acacaatatg taacgtcggt
     2821 ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata aagatcgtca
     2881 ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca
     2941 caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt tttagcgtta
     3001 cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg
     3061 aggtgtacca gcatgttccg gcagataccg gtctgggcac gctggcaaaa cgctatctgg
     3121 gacgctacgg tcaatggctg acgggcttca gtatgatgtt cttaatgtat gctctgactg
     3181 cagcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac tggacaggta
     3241 tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt
     3301 gtgttggaac atcgctggtc gatttattta accgttttct gttcagcgcc aaaattattt
     3361 ttctggtggt gatgctggta ttgctgctac cgcatattca caaagtgaat cttttaaccc
     3421 tgccgttgca acaggggctg gcactgtctg caatcccggt gatttttacg tcgtttggtt
     3481 ttcacggtag cgtgccgagt attgtcagct atatggatgg caacattcgt aagctacgct
     3541 gggtgtttat aatcggtagt gccatccccc tggtggcata tattttctgg caggtggcga
     3601 cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat gctggattaa
     3661 acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc
     3721 atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta ggcttatttg
     3781 attatctggc tgatttgttt cagcgttcaa ataccgttgg tggacgattg caaactggtg
     3841 caattacctt tctgccgccg ttggcgtttg cactgtttta tccacgagga tttgtgatgg
     3901 cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct tcactattga
     3961 cctggcaaag cagaaagcac aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg
     4021 cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg
     4081 cagggttgtt accagaagtg gggtgatcag atagcctcaa attccttatt gggtgccaga
     4141 attaacgctg acacccaatg tgacctctta atgcaggcag cactgcttaa atttcttacc
     4201 actgccgcac ggacaaggat cgttacgccc cggtttctct tctgctttga tcggttgctg
     4261 aacagctttt tcctgcggat gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg
     4321 aatggcatct acgctctctt caaacgcttc tggcgacatt ttctctaccc gctcgaagtt
     4381 ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaaagaat cgggcaacgt
     4441 tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct caacaatcgt
     4501 cagctcgctg ccatcaactt ctcgcaagcc gaataacggc tcaaactgct ccgggaattc
     4561 gttcagacgc tctgcggtat cggccatatg ttgaaaagcc aggttcataa agcgcgtcat
     4621 ctctttctct gacgcccaac gcggcacata gtcagcccca ccccacacgg caaccagcca
     4681 ctgttctggt tcaatctctt gcggagaact caacaccgcc gtcaataaac cgtccagctc
     4741 cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt ccagccattc
     4801 caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga tctcaactcg
     4861 ccagcaccta tcttacatgc cggtccgtat cagagatact ttttgagtgg ctttgccggt
     4921 gattaaaaat taaggagggt gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt
     4981 ggtttcacga cactgcttat tactttgatt ttattcttat ctttcccatg gtacccggag
     5041 cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg tgtctaccga
     5101 ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga
     5161 cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc aggcctttca
     5221 gccagacatc cgcttgacgc cgatgtcttt taaactggag cgggaaacga gactcgaact
     5281 cgagacctcg accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa
     5341 gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg
     5401 ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt
     5461 gctgaaaatt acgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat
     5521 attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt
     5581 actcaaccca aatgttcgga cgccacagca gccaggccaa cgccaccatc tgggcagtgg
     5641 ttttcacttt cccaatccag gagacggcca cgctactgcg tttacccaac tccgccatcc
     5701 attcgcgtag cgcagaaata ataatttcac gggcgatcat cgttgccgcc ggtaatgtca
     5761 cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg agaactttat
     5821 ctgccacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg cgtgccagaa
     5881 aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg
     5941 accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg acacggaaca
     6001 gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa
     6061 gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc
     6121 cagaccttgc gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc
     6181 ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga cgccttcaat
     6241 ggtttccagg gaactggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc
     6301 gtgatcatgt gattcatcgc gaatatgctg gataacatgc agcgcgggtg aatctggcgg
     6361 caaactaaat ccctcacctt ccggctcaaa gaacagcgtt tccagtccag ccttacgatc
     6421 tgctcctttg gcaacgccaa gtagcagcgg atgattttta tcccatgaga catccagttc
     6481 ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc
     6541 cgggatctta ctgtcgtcaa tggctttacc ataacgccga cgcagcacct gattcatcgc
     6601 cgcataatca tcgcccggcg tgatgcctgt aatgttatag cgccgatact ccgcacgcag
     6661 cgggccgtta gcatcaaaca ccacacagga agcgacggtt tgttcgccca tggtatggct
     6721 gatgtcaaag cactccatcc gcttcacttc cggcaatttc aacacgctgg caagcgcggt
     6781 cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt
     6841 ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga ggtttggttt gaacattaat
     6901 cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa
     6961 atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactgacc
     7021 tacgaaggtt tctaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct
     7081 gcggctgccg agcactttgc cctgacgaat gaacaatacg tggacacaag ccatgcccgc
     7141 atcgaacgcc acaccaataa cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc
     7201 ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacat gccgcttctt caaactccag
     7261 attctggctg gcagtttcca tacggctaat gagttgcgta agcacctgat catctttgcc
     7321 agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc
     7381 ttcaacgcac ggtcccagac agcgccctat ctggtattgc agacacggac gcgagcgatt
     7441 gcgataaaca ctattttcgc actggcgaat ggggaaaatc ttttgcagta gcgccagtgt
     7501 ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttgg catgctttgc
     7561 accacgatgc atcgccagac gcgggtgggt atcgccactc aggaagataa aaggataaga
     7621 tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt agttgtgttc
     7681 cagcaacagc gcttcggttt ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc
     7741 gaccagcgct tcggttttgc gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt
     7801 tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt acatgcgata
     7861 aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac
     7921 tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca
     7981 acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc
     8041 ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttagt gatcatcagc
     8101 ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg
     8161 atctggctta acgccatttg ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag
     8221 acagaacgaa tcgcactcac gacttcctgc ggagccgcgc ctttgctgag gtagccagca
     8281 gcacctgcct gcatgacttt cgctggtaaa gggttttctg tatggaccgt aagcatgatg
     8341 attttaacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc
     8401 ggcatactca tgtccattag caccacgtca acggcatttg tccggcacca cttaacggcg
     8461 tcttcaccgc acgatgcctc tccgacgact tttataccct taatatcttc cagaatgcgt
     8521 cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata
     8581 tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag cggtttttat
     8641 tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtcaattc
     8701 gattgtccac agttatggtc acgcccgtca ccaacggctt accggatcgt ttcacagtaa
     8761 gccctacgat gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa atcagcccgc
     8821 tgaatatgta acattaatta acccgaacaa tacgggcaaa aaacattca
//
LOCUS       DAZPDE010000153         8778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPDE010000153 DAZPDE010000000
VERSION     DAZPDE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.156"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHU0J_003235"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHU0J_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..2289
                     /locus_tag="ACHU0J_003236"
                     /pseudo
     CDS             362..2289
                     /locus_tag="ACHU0J_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2273..2476
                     /locus_tag="ACHU0J_003237"
     CDS             2273..2476
                     /locus_tag="ACHU0J_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR1217804.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2473..4066
                     /locus_tag="ACHU0J_003238"
                     /pseudo
     CDS             2473..4066
                     /locus_tag="ACHU0J_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4056..5561
                     /locus_tag="ACHU0J_003239"
     CDS             4056..5561
                     /locus_tag="ACHU0J_003239"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1217805.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5598..5945
                     /locus_tag="ACHU0J_003240"
     CDS             5598..5945
                     /locus_tag="ACHU0J_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1217806.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            6003..7031
                     /locus_tag="ACHU0J_003241"
     CDS             6003..7031
                     /locus_tag="ACHU0J_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1217807.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHU0J_003242"
     CDS             7083..7469
                     /gene="gpFI"
                     /locus_tag="ACHU0J_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1217808.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7481..7858
                     /locus_tag="ACHU0J_003243"
     CDS             7481..7858
                     /locus_tag="ACHU0J_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1217809.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7845..8429
                     /locus_tag="ACHU0J_003244"
     CDS             7845..8429
                     /locus_tag="ACHU0J_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1217810.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8426..>8778
                     /locus_tag="ACHU0J_003245"
     CDS             8426..>8778
                     /locus_tag="ACHU0J_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1217811.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
BASE COUNT         2050 a         2070 c         2690 g         1968 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg caaggatgca
     2221 actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt gaatggttac
     2281 agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt
     2341 tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc tgaatgagct
     2401 taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc gccgaccgct
     2461 gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt
     2521 gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc agagtggtta
     2581 ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg gaatgcccgg
     2641 gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct gcataaggat
     2701 catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg gctggggatg
     2761 cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc ggaatacgca
     2821 gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga atttatccgt
     2881 gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt ctgggatacg
     2941 gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg ggtggacacg
     3001 ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga tcggtatggg
     3061 cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc cggacgctgg
     3121 gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat ttttgagccg
     3181 gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga gcggatgaag
     3241 atgctggact ccctgcaggc cacgcagctt cagtcggcca ttgtgaaggc gatgtatgca
     3301 gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc gggggcaccg
     3361 caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg ctggtatgac
     3421 acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg gcgatgcgct
     3481 gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac aggcgctgct
     3541 gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa
     3601 ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt ttatggggcg
     3661 gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc tggaagaggc
     3721 gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc aggcgcgttc
     3781 agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg ggctcaagga
     3841 ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga aagaactggc
     3901 actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat ccgcagagcg
     3961 cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc agcagatagc
     4021 ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt tcacatatta
     4081 tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt
     4141 gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag caggtacagc
     4201 ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc aaacgacctg
     4261 cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc acgctggtac
     4321 accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc attgtcgcct
     4381 gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac attgacagtt
     4441 ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt ctccggcagc
     4501 tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg ttactggcat
     4561 cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt ggggtgatga
     4621 tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc acgctgattt
     4681 acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca aaggttcgcc
     4741 aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa gtggcgatgt
     4801 ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt gaaggtcagt
     4861 ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc atcagcgtga
     4921 tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta actgcaacgg
     4981 atgctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag gaagcgaaag
     5041 gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt gagcaggccc
     5101 gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag agtgaagccg
     5161 atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca acgctggcgg
     5221 cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca ccgcaggcga
     5281 atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa ggggcagagg
     5341 cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc cgggctgtac
     5401 tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc atgtttgaac
     5461 atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag acgtcagcag
     5521 acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa
     5581 tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg gaagtacgta
     5641 tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt tcctcggcaa
     5701 caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg gtctgggacg
     5761 gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt acagagacgg
     5821 tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg cctgagagag
     5881 tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac gcagcgctgc
     5941 cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa aggaactgat
     6001 ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa gttaaatttc
     6061 gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc gaagaggtaa
     6121 tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg gttgttgaag
     6181 gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc tacgttaagc
     6241 cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa gatccggccc
     6301 agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc aaacaggaag
     6361 agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg tatggcaaat
     6421 acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg tcggtgggaa
     6481 ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat acctttgatc
     6541 cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat attgccatta
     6601 tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa aaactggata
     6661 cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg gcggtggtgt
     6721 ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc tatgtggcag
     6781 aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga aatacggccg
     6841 cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct gaaggcgtgg
     6901 tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg cgtgaattca
     6961 ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc
     7021 aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa tcaggagcgg
     7081 gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag aggctggggc
     7141 gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg gaactggagg
     7201 aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca tcgaaagatg
     7261 atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt accggagacg
     7321 aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt aatggttcag
     7381 tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct gccagcatgg
     7441 cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt tcgataatct
     7501 gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg ggatcagcgc
     7561 cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg atgatcccga
     7621 aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt gcctgtttgt
     7681 gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg gggatgattc
     7741 tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc ggttaaggcg
     7801 gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag ggacttgaga
     7861 atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc agcgcatggg
     7921 cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt gcgcagaata
     7981 ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg gagcgtgtca
     8041 ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt aaccggggta
     8101 acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc cggcatggca
     8161 gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc cgggatgcgt
     8221 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac ggcaaaaatc
     8281 gttaccccat tgatgtggtg aaaatcccga tgtccggacc gctgacacag gcatttgaag
     8341 atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat gccctgaaac
     8401 aacaactgag gttacatctg agtaaatgaa ccgacacaca caaatccgtc agagcgtgct
     8461 ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc ttccggcatt
     8521 tattgatgcg caggaactgc ccgccgtggc ggtgtggctg agtgatgcgc agtacaccgg
     8581 aaaaatgacg gatgaggacg actggcaggc tgttctgcat attgccgttt tcgtccgtgc
     8641 acaggcgccg gattctgagc tggatacgtg gatggagaac accatatttc cggccatgaa
     8701 ggatattccg gtgctttccg gactcatcga caccatgatc ccactcggtt ttaactatca
     8761 acgtgataat gagatggc
//
LOCUS       DAZPDE010000154         8739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPDE010000154 DAZPDE010000000
VERSION     DAZPDE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.157"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            135..671
                     /gene="hpt"
                     /locus_tag="ACHU0J_003246"
     CDS             135..671
                     /gene="hpt"
                     /locus_tag="ACHU0J_003246"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1217812.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(712..1374)
                     /gene="can"
                     /locus_tag="ACHU0J_003247"
     CDS             complement(712..1374)
                     /gene="can"
                     /locus_tag="ACHU0J_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1217813.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1483..2409
                     /locus_tag="ACHU0J_003248"
     CDS             1483..2409
                     /locus_tag="ACHU0J_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1217814.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2406..3176
                     /locus_tag="ACHU0J_003249"
     CDS             2406..3176
                     /locus_tag="ACHU0J_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1217815.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3281..3721
                     /locus_tag="ACHU0J_003250"
     CDS             3281..3721
                     /locus_tag="ACHU0J_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1217816.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3785..5014
                     /locus_tag="ACHU0J_003251"
     CDS             3785..5014
                     /locus_tag="ACHU0J_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1217817.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5018..5398)
                     /gene="panD"
                     /locus_tag="ACHU0J_003252"
     CDS             complement(5018..5398)
                     /gene="panD"
                     /locus_tag="ACHU0J_003252"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1217818.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5672..6574
                     /gene="rpnC"
                     /locus_tag="ACHU0J_003253"
     CDS             5672..6574
                     /gene="rpnC"
                     /locus_tag="ACHU0J_003253"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1217819.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(6648..7499)
                     /gene="panC"
                     /locus_tag="ACHU0J_003254"
     CDS             complement(6648..7499)
                     /gene="panC"
                     /locus_tag="ACHU0J_003254"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1217820.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(7511..8305)
                     /gene="panB"
                     /locus_tag="ACHU0J_003255"
     CDS             complement(7511..8305)
                     /gene="panB"
                     /locus_tag="ACHU0J_003255"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1217821.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8418..>8739)
                     /locus_tag="ACHU0J_003256"
     CDS             complement(8418..>8739)
                     /locus_tag="ACHU0J_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1217822.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
BASE COUNT         2104 a         2048 c         2296 g         2291 t
ORIGIN      
        1 atcacaattc gagctttatt aacagattcc gcgaatgaat agttttactg gtatactgcg
       61 cgtcttgcgc tttgttgcgg tgccaaaacc tgcccgtgcg aagtgatttg tttttaaatc
      121 atatggttag agatatgaaa catactgtag aagtaatgat ccccgaagcg gagattaaag
      181 cgcgtatcgc cgaactgggt cgtcagatta ctgagcgtta caaagacagc ggcagcgata
      241 tggtgctggt gggtctgctg cgtggctcat ttatgtttat ggcggacctg tgccgtgaag
      301 ttcaggtatc tcatgaagtc gactttatga ccgcctccag ctacggtagc ggcatgtcca
      361 ccacccgtga tgtgaaaatc ctcaaagatc tggatgaaga tatccgtggc aaggacgtgc
      421 tgattgttga agatatcatc gactcgggga atacactgtc gaaagtgcgt gagatcttaa
      481 gcctgcgcga accgaagtcg ttggcgattt gtacgctgct ggataaaccg tcccgtcgtg
      541 aagtgaacgt cccggttgaa tttatcggtt tctcgatccc ggatgagttt gtggtgggtt
      601 acggcattga ttacgcacag cgttaccgtc atctgccgta tattggcaaa gtgattctgc
      661 tggacgagta agtgtgaagt tgccggatgt gttgcatccg gcatggcatt tttatttgtg
      721 gttggcgtgt ttcagcttga ggttggaaat cccgtgacgg taacgttgct caagggtttc
      781 gcggttggtg gcggtaacat ccagatcacg cagcaagccg tcgtgaatgc cgtaggccca
      841 gccgtgaatg gtaactttct gcccgcgttt ccacgctgat tgcataatgg tggagtggcc
      901 caggttatac acctgttcca tgacgttcag ttcacacaag gtatccagac ggcgctcttg
      961 cggcatttcg ccgagcaatg agctatgttt gaaccagata tcgcggatat gcagcagcca
     1021 gttgttgata agccccagtt ccgggttttc aacggcggct tgtacgccgc cgcaaccgta
     1081 gtggccacag ataataatgt gttcaacttc gagtacatcc actgcatact gaaccacgga
     1141 aaggcagttc aggtcggtgt gaatgactag gttagcaaca ttacggtgaa caaagagttc
     1201 gcccggctca agaccggtta aacgttctgc aggaacgcga ctgtcggaac atccaatcca
     1261 tagaaagcgc ggtttttgcg cttgtgccag tttctcaaaa aacccgggat cctcttccac
     1321 cagcattttt gaccatagtg cattgttgct gatgagtgta tctatgtctt tcatggaggt
     1381 taacgacctg taaccaaata attacgtttg gctaatatag ggcaactccg ggacgattta
     1441 aaccacagat aaagtgtaag aacgtaaggt aagtaaaaat ttatgaccat tgcactggaa
     1501 cttcaacagc ttaaaaaaaa ctatccaggc ggcgttcagg cgcttcgtgg aatagatttg
     1561 caggtcgaag cgggtgattt ttatgcgctt ctcgggccga acggggccgg gaaatcgacc
     1621 actatcggta ttatcagctc tctggtaaat aaaacctccg ggcgggtcag cgtatttggt
     1681 tacgatctcg agaaagatgt cgtgaacgct aaacgtcagt tgggactggt gccgcaggaa
     1741 tttaacttca acccgtttga aaccgtgcag caaattgtgg tgaatcaggc agggtactac
     1801 ggtgtggagc gcaaagaggc gtacatccgc agcgaaaaat atcttaagca gctcgattta
     1861 tggggaaaac gcaacgaacg tgcgcgtatg ttatctggcg ggatgaagcg ccgtttaatg
     1921 attgcccgtg cgttaatgca tgaacctaaa ctactgattc tcgacgaacc gaccgcaggc
     1981 gtggatattg aacttcgccg ctcaatgtgg ggctttttga aggatttaaa cgacaaaggc
     2041 accaccatca ttcttaccac acactacctg gaagaagcag aaatgctgtg ccgcaatatc
     2101 ggcattattc aacatggtga gctggtggaa aatacctcga tgaaggcgct gctggcgaag
     2161 ctgaaatcgg aaacctttat tctcgatctc gcaccgaaaa gtccgttacc gaagctcgat
     2221 ggctatcagt atcgactggt cgataccgcg acgctggaag ttgaagtgct acgtgagcag
     2281 gggatcaaca gcgtatttac gcagttaagt gagcagggca ttcaggtatt aagtatgcgt
     2341 aacaaagcta accgtctgga agagctgttt gtttcactgg ttaatgaaaa acaaggagat
     2401 cgcgcatgat gcatctttac tgggtggcgc taaaaagcat ctgggcgaaa gagatccatc
     2461 gctttatgcg tatctgggtg cagacgctgg tgccgccagt catcaccatg accctttact
     2521 ttattatctt cggtaacctg ataggttcgc gtattggcga tatgcatggc ttcagctata
     2581 tgcagttcat cgtaccgggg ctgatcatga tgtcggtgat caccaacgcc tacgctaacg
     2641 tcgcgtcatc attttttggt gccaagttcc agcgtaatat tgaagagctg ctggtagcgc
     2701 cggttccgac tcacgtcatt attgccggat atgtcggcgg tggcgtggcg cgtggtctgt
     2761 ttgttggcat tcttgtgacg gcaatttcac tgttttttgt gccgtttcag gtgcattcgt
     2821 gggtattcgt tgccttaacc ctggtgctca cggcggtgct attctccctt gcgggtttgc
     2881 tgaacggcgt gtttgccaaa acgttcgatg acatcagcct ggtgccaacc tttgtgttaa
     2941 cgccactcac gtatttgggc ggggtctttt actcactgac tttgttgccg ccgttctggc
     3001 aagggctgtc gcacctgaac ccaatcgttt atatgatcag tggtttccgc tacggcttcc
     3061 tcggtataaa tgatgtgccg ctggtcacta cctttggcgt actggtggtc tttattgtgg
     3121 cgttttattt gatctgttgg tcgctgatcc aacgtggacg tggtttgcgt agctaaggct
     3181 atttcctctc ctctggattc gggggagagg agttttgacg gctatcaccc tttatcaaca
     3241 atggtcaggg taggctgatt ttcggctaag gaggaaggcg atgttaggtt gggtaattac
     3301 ctgtcacgat gaccgggcgc aagagatact ggatgcgctg gagaaaaaac atggggcact
     3361 tcttcagtgc cgggccgtga atttctggcg cggattaagc tctaatatgc tcagccgcat
     3421 gatgtgcgat gctctgcatg aagcggactc tggtgagggt gtcatcttct taaccgatat
     3481 agccggagcg ccaccgtatc gcgtggcttc attattaagc cacaaacact cccgttgcga
     3541 agtgatttct ggtgtcacgt taccgttaat tgaacagatg atggcttgcc gtgaaaccat
     3601 gaccagttca gagtttcgtg agtgtattgt cgaactgggt gggccggagg tgagtagtct
     3661 ttggcaccaa caacaaaaaa atccgccttt cgtcctcaaa cataatttgt atgagtatta
     3721 acccgcgatt ctgatggcgc ttttgctaca ataaaagcgt tgtttcaccc tcggttattt
     3781 tttcatgtat aaacaagctg ttattctcct gctgatgctg tttaccgcaa gtgtcagtgc
     3841 cgcgttacct gcccgttata tgcaaaccat cgaaaatgct gcggtctggg cgcaaattag
     3901 tgacaagatg gtgaccgtgg ggaatattcg ggccggacaa atcattgccg tggagcccac
     3961 tgccgcaagt tattacgcat ttaattttgg ctttggtaaa gggtttatcg ataaagggca
     4021 tctcgagcca gttcagggac gacaaaaagt tgaagacggt ttgggtgacc tcaacaagcc
     4081 gctgagtaat cagaacttaa ttacctggaa agatacgccg gtctacaacg cgccgagtgt
     4141 gggcagtgcg ccatttgggg tactggcgga caatttgcgc tacccgattt tgcataaact
     4201 gaaagacagg ttaaatcaaa cctggtatca gatccgtatt ggcgatcgac tggcctatat
     4261 cagcgcgctg gatgcccaac ccgataatgg cctgccggtg ctaacttatc accatattct
     4321 gcgcgacgaa gaaaacaccc gttttcgcca tacttcgacg accacctcgg tacgcgcttt
     4381 caataaccaa atggcctggc tgcgcgacag gggatacgcg acactgagca tggctcagct
     4441 ggaaggttac gtgaagaata cgatcaatct ccctgcgcga gcggtggtga ttacctttga
     4501 tgatggcctc aagtcggtga gccgctatgc gtatcctgtg ttgaaacaat atggcatgaa
     4561 ggcgacggtg tttattgtta cctcacgcat caaacgtcac ccgcagaagt ggaacccaaa
     4621 atcgctgcaa tttatgagcg tttctgagct taacgaaatt cgcgatgttt ttgatttcca
     4681 gtcacatacc cattttttgc atcgggtaga tggttatcgc cgacccatat tactgagccg
     4741 tagtgagcac aatattctgt ttgattttgc acgttcacgc cgcgctctgg cgcaatttaa
     4801 tccacatgtc tggtatcttt cgtatccgtt tggcggcttt aatgacaaag ccgtgaaggc
     4861 agcaaacgat gccggatttc acctggcggt gacaaccatg aaaggcaaag taaaaccggg
     4921 ggataatccg ttgttactaa aacgacttta tatcttaaga acggattcgc tggagaagat
     4981 gtcgcggctg gtgagtaacc agccgcaggg ataacaatca agcaacctgt accggaatcg
     5041 ctttcgcggt acgtttcatt tcattgtcgc cttcaaaata ggcgacattg ggtcgccagg
     5101 tgcgagcttc ttcatctggc atggtaacga agctggcgat gatgacaata tcgccgacgc
     5161 tggcgcagtg ggccgccgca ccgttaacag aaataattct cgaaccgcgt tctgccgcga
     5221 tggcataagt ggagaaacgc ttgccgttgg tgacattcca gatatcgatg gcttcgtttt
     5281 cgagaatacc ggctgcgtca agaaaatcct ggtcaatggc gcaagaacct tcatagtgca
     5341 ggtccgcatg agtcactttc acgcggtgga gtttgccctg cagcatcgtg cgaatcataa
     5401 cttctacctt tctaccctgt cgttaacgaa gcaggcggtg cctgctttga ggaaattctc
     5461 acgcagtatt gcccgatttt tgatgagtgt ctactcatct gatggcattt gcgtcagcag
     5521 tttgcgtacc gcgccgaagc gtgccatgta acgcctgtaa cgaattgata tttttatgaa
     5581 cgctgctcgg taaacaataa agaggtggct gacgatagca cgccttgact ggatactgcc
     5641 ttcacgcaag gccacacgga agacagggaa gatggatgca ccgagtacca caccgcatga
     5701 cgcggtattt aaacaatttt taatgcatgc ggagacggct cgcgactttc tggagataca
     5761 tttgccagtg gaattacgcg aactttgtga tctcaacacg cttcatttag agtcggggag
     5821 tttcattgaa gagtgcctga aaggtcacag cacggacgtg ctctattcca tgcaaatgca
     5881 gggcaatccc ggttatctgc atgttgtgat tgaacaccaa agcaagccgg ataagaaaat
     5941 ggcctttcgc atgatgcgtt attctattgc tgccatgcac cggcatctgg aggctggcca
     6001 cgataagctg ccgctggtgg tgccgatact gttttatcag ggcgaggcca caccttatcc
     6061 gctctcaatg tgctggtttg atatgtttta ctcgccggag ctggcgcgac gcgtctataa
     6121 cagtcctttc ccgctggtgg atatcaccat cacaccggat gacgaaatca tgcaacatcg
     6181 gcggattgcg attctcgaac tactgcaaaa acatattcgc cagcgcgact taatgttatt
     6241 gctggagcaa ctggtcacgc tgattgacga agggtacact agcggaagtc agttagttgc
     6301 catgcaaaac tatatgctgc aacgcggtca tactgaacaa gcggatttgt tttatggtgt
     6361 gctgagagac agggaaacgg gagggaagtc tatgatgacg ctggcgcagt ggtttgaaga
     6421 gaaagggatt gagaagggga ttcagcaggg aagacaggag gaaaggcaag aattcgccct
     6481 gcgtcttcta agtaaaggaa tgtctcggga agacgttgca gagatggcaa atttacctct
     6541 tgctgagatt gataaggtaa ttaaccttat ttaagttacc tgtgttatga cagatgacgt
     6601 ggggtaaatt aataactggc gccatcagcc gtagcgccag ttaagtatta cgccagctcg
     6661 accattttgt tgtcgatcag gcgagcatcg ccaagccagg cggctaccag aattactgcc
     6721 cgtttgctgg tttcagaaac ttccagcaat gtgtcggcat cgcgaatctg aatatcatcg
     6781 gcgcggaagc ctttttcatt cagttcttgc cccgcaatgg taataatttc atcgagatcc
     6841 cgttccccag cctgcaattt gtcagcaatc gaacttaaaa ctttgtacag accaggcgca
     6901 attttgcgtt gttccgccgt cagataaccg ttacgggaac ttagcgccag accgtctttg
     6961 gcgcgcataa ttggcacacc gacaatctca atatcgaagc ccatatcggc aaccattttg
     7021 cggatcaacg ccagttgctg aaaatctttt tcaccgaagc aggcgatgtc cggctggacc
     7081 aggttgaaca gcttgctgac aatagtcgaa acgccgcgaa aatgtcccgg acggctggca
     7141 ccttccagca tggtcgaaag gccaggaacg tcaacgtaag tgtgggtttc agtaccgttt
     7201 gggtagatct cttttaccga aggggcgaaa actaaatcca ctttacgttt gtttagcttc
     7261 tcgcagtcct cctgcaaggt ccgtggataa cgtgccagat cttccgggcg gtcgaactgc
     7321 atcgggttaa cgaaaatact gacgacgacc acatcggcgc gggctttggc ttcgtcgacc
     7381 agcttcatat ggccattgtg cagattaccc atagtaggca ccagcgccac gcgcttgcct
     7441 tccatacgca ggcggcgaat ttgctgacgc agcagcggca gggtttcgat aattaacaca
     7501 acgtgactcc ttaatggaaa ctgtgttctt cgcccggata aacgccggac tccacttcag
     7561 ccatatactg ccgcacagcc gcgcggatgt cgcccgtttc ggcgaggaaa tttttagcga
     7621 atttaggaat gtgaccgccg gtaataccaa aggcgtcgtg catcacgagg atttgcccgt
     7681 cagtgacgtt gcctgcacca ataccaataa ccgggatcgc cagtgcttcg gtaatacgtt
     7741 ttgccagctc aaccggcacg cattccagca ccagcagctg tgcccccgcg gcttctaagg
     7801 ctaatgcatc gctgagtagt cgatcgctcg cttcattgcc gcgcccctgt actttgtagc
     7861 caccgaaaat attcactgac tgtggggtta aacctaagtg accacataca ggaacggcac
     7921 gttcggtcag catttttacg gtttcgacca gccactcacc gccctcaatt ttgaccatat
     7981 tggcaccggc acgcataacc gttgcggcgt tttcaaaggc ttgttccggc gtggcatacg
     8041 ccataaacgg caggtcagcc aatagcaggc agtttggtgc gccgcgacgt acggcggcag
     8101 tgtggtaggc gatatcagca acggttactg gcagagtgga atcgtggccc tgaacggtca
     8161 tgcccagcga gtcgcctacc agcatgacgt taagcccttc atcagcaaat agtttcgcga
     8221 agctgtagtc gtaagcggtg atggtcgcga aacgcttttt atcctgctta tatttctgaa
     8281 gtgaagcaat ggtggtcggt ttcataacgt atcctgataa atgatgtcgt gttgcctggc
     8341 attttatcag tcacattggt gggggcaatg atttatcaat taccgcaccg ccagatgatg
     8401 gcggtgcggt ggacttatta agggtaggtt acctggaaag tactggtggc tttaaactca
     8461 ccagcttcta ttggtatagt tccatcctgt tgtagcgtgg cctggaaatg gagagggtat
     8521 gttatacctg tatcttgatc cgggtaaact cctcctgttg tatcgtcttt gcctctcggg
     8581 tcgtaatctt tataaacaga atttgaatca ttaggtttca atgtcattag aggattttta
     8641 ctagttgcta aaccttctat gagtacaccg actccttttg ctgcatcatt acctgtgaga
     8701 gtattaccaa ggagttgccc gttttcagta ccaaccttt
//
LOCUS       DAZPDE010000155         8732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPDE010000155 DAZPDE010000000
VERSION     DAZPDE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.158"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(435..641)
                     /locus_tag="ACHU0J_003257"
     CDS             complement(435..641)
                     /locus_tag="ACHU0J_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1217823.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACHU0J_003258"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACHU0J_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1217824.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHU0J_003259"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHU0J_003259"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1217825.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHU0J_003260"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHU0J_003260"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHU0J_003261"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHU0J_003261"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1217826.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHU0J_003262"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHU0J_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHU0J_003263"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHU0J_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1872 a         2244 c         2503 g         2113 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tc
//
LOCUS       DAZPDE010000156         8706 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPDE010000156 DAZPDE010000000
VERSION     DAZPDE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.159"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHU0J_003264"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHU0J_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1217827.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHU0J_003265"
     CDS             696..914
                     /locus_tag="ACHU0J_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1217828.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHU0J_003266"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHU0J_003266"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1217829.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHU0J_003267"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHU0J_003267"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1217830.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHU0J_003268"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHU0J_003268"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1217831.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHU0J_003269"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHU0J_003269"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1217832.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHU0J_003270"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHU0J_003270"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1217833.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHU0J_003271"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHU0J_003271"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1217834.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHU0J_003272"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHU0J_003272"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1217835.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1998 a         2163 c         2465 g         2080 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttattat gatgcaaaag
     8701 taacac
//
LOCUS       DAZPDE010000157         8528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPDE010000157 DAZPDE010000000
VERSION     DAZPDE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.160"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHU0J_003273"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHU0J_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1217836.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHU0J_003274"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHU0J_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1217837.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHU0J_003275"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHU0J_003275"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1217838.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHU0J_003276"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHU0J_003276"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1217839.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHU0J_003277"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHU0J_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1217840.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHU0J_003278"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHU0J_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1217841.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHU0J_003279"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHU0J_003279"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1217842.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZPDE010000158         8495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPDE010000158 DAZPDE010000000
VERSION     DAZPDE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.161"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            44..397
                     /locus_tag="ACHU0J_003280"
     CDS             44..397
                     /locus_tag="ACHU0J_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1217843.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHU0J_003281"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHU0J_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR1217844.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHU0J_003282"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHU0J_003282"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1217845.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHU0J_003283"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHU0J_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1217846.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHU0J_003284"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHU0J_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1217847.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHU0J_003285"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHU0J_003285"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1217848.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHU0J_003286"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHU0J_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1217849.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACHU0J_003287"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACHU0J_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1217850.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACHU0J_003288"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACHU0J_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1217851.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8495
                     /locus_tag="ACHU0J_003289"
                     /pseudo
     CDS             7609..8495
                     /locus_tag="ACHU0J_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1904 a         2307 c         2287 g         1997 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggca
//
LOCUS       DAZPDE010000159         8371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPDE010000159 DAZPDE010000000
VERSION     DAZPDE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.162"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHU0J_003290"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHU0J_003290"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1217852.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHU0J_003291"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHU0J_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1217853.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHU0J_003292"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHU0J_003292"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1217854.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHU0J_003293"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHU0J_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1217855.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHU0J_003294"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHU0J_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1217856.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHU0J_003295"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHU0J_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1217857.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHU0J_003296"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHU0J_003296"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1217858.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2122 a         2126 c         2113 g         2010 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt t
//
LOCUS       DAZPDE010000160         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPDE010000160 DAZPDE010000000
VERSION     DAZPDE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.163"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(168..566)
                     /locus_tag="ACHU0J_003297"
     CDS             complement(168..566)
                     /locus_tag="ACHU0J_003297"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR1217859.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHU0J_003298"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHU0J_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR1217860.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6128
                     /gene="traI"
                     /locus_tag="ACHU0J_003299"
                     /pseudo
     CDS             875..6128
                     /gene="traI"
                     /locus_tag="ACHU0J_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="ACHU0J_003300"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="ACHU0J_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1217861.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="ACHU0J_003301"
     CDS             6949..7509
                     /locus_tag="ACHU0J_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1217862.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="ACHU0J_003302"
     CDS             7640..7852
                     /locus_tag="ACHU0J_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1217863.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="ACHU0J_003303"
     CDS             8096..>8332
                     /locus_tag="ACHU0J_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1217864.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2670 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggtgc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAZPDE010000161         8250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPDE010000161 DAZPDE010000000
VERSION     DAZPDE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.164"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(384..1580)
                     /gene="hemY"
                     /locus_tag="ACHU0J_003304"
     CDS             complement(384..1580)
                     /gene="hemY"
                     /locus_tag="ACHU0J_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1217865.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1583..2788)
                     /gene="hemX"
                     /locus_tag="ACHU0J_003305"
     CDS             complement(1583..2788)
                     /gene="hemX"
                     /locus_tag="ACHU0J_003305"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1217866.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2810..3550)
                     /gene="hemD"
                     /locus_tag="ACHU0J_003306"
     CDS             complement(2810..3550)
                     /gene="hemD"
                     /locus_tag="ACHU0J_003306"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1217867.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3547..4488)
                     /gene="hemC"
                     /locus_tag="ACHU0J_003307"
     CDS             complement(3547..4488)
                     /gene="hemC"
                     /locus_tag="ACHU0J_003307"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1217868.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4875..7421
                     /gene="cyaA"
                     /locus_tag="ACHU0J_003308"
     CDS             4875..7421
                     /gene="cyaA"
                     /locus_tag="ACHU0J_003308"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1217869.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7461..7781)
                     /gene="cyaY"
                     /locus_tag="ACHU0J_003309"
     CDS             complement(7461..7781)
                     /gene="cyaY"
                     /locus_tag="ACHU0J_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1217870.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7829..>8250)
                     /locus_tag="ACHU0J_003310"
     CDS             complement(7829..>8250)
                     /locus_tag="ACHU0J_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR1217871.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1840 a         2177 c         2138 g         2095 t
ORIGIN      
        1 gtaggatcaa gctcaggtta aatattcact caggaagtta ttactcagga agcaaagagg
       61 attacagaat tatctcataa caagtgttaa gggatgttat ttcccgattc tctgtggcat
      121 aataaacgag tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg
      181 gaatgacgca gagccgttta cggtgcttat cgtccactga cagatgtcgc ttatgcctca
      241 tcagacacca tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt
      301 taacgcctgc tccgtaataa gagcaggcgt ttttttatgc atcagaaagg tcccggaggt
      361 gctttcctcc gggtgagaag gaactactgt ggcgggttat tctgcaacgt taacatcaaa
      421 ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca
      481 agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ccgcgcggaa ggcgagcgat
      541 gcttcctgcc attcaccgtg cttcatcagt gactgaccca atgtgctcca caacagcggg
      601 cgatcaccga cgtttttgat ttgctggcgt agcacttttt ccagctgttc cgggttattg
      661 gttttcagac gcgggatcgg cagcagtagg cgatcgtcat actggcgttt caggccgtcg
      721 atgataattt gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc
      781 acctgcaacg ctacctgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa
      841 ccttcactac cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc
      901 agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc
      961 agcagagaac tccatgcccc tgtgcggata tacgcctgtt ccgccagacg taatacttca
     1021 ggatggcgtg gcgtaacttc cagcagctta tccacgccgt ggcgtgcagc atggttttca
     1081 ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc
     1141 agctccgctg cgcgttccag atgttggttg gcgcgtgctt catcaccacg ttgttgcgcg
     1201 gcttcggcag ccagtagata gttcaccacc ggttgttcag cgtgatcggc atttttcgcc
     1261 atcagctttt caacttgctg ataatcacct tccgccagtt tcagcagcgc ctgttcggtc
     1321 tgcttacgcg cacggcgacg cttacgtccg acaaaccatc cacgggtgtg cgcgccagtg
     1381 cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaat
     1441 atgatcgcca ggcccgtgac gctggtttcg atattgtagt tgtcggtctg gatcagcaca
     1501 taaccctgat gaccggcaat catcgggcca accactatcc ccgcaatcag caaaacaaag
     1561 agtaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcacctt
     1621 gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tttagcttcc gttgtccccg
     1681 ctgccggttg tgccagcagg ttacgcacgc gagtctgcat cagtttttcc agcatcgcct
     1741 ggctttgcag ggtttccgga agatccatcg agatattttg ctggcttaac tggtccacct
     1801 cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta gtaagcacgt acccaggtgg
     1861 agacgttctc cagcgcctgg cgataagtct cttcctgatg acgcggtaca gcttgtgctg
     1921 cgaccagcag gcgagagcga atattttcgc gcagatagat atcctgattt ggcgctaaca
     1981 gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa gttgtccata aagttctgcc
     2041 agcttttttg cagattgata cgccattcgc tgatggaact ggaaagctct tcaccgtctg
     2101 aatccatcgg cgaaccatcg ctgtcattat cggccagacg caggttatct acctgatttg
     2161 aaagctgatt aagcttaagg atgatgccgt cataatccac ctgcgatact gcagaaaggc
     2221 tggcgatatc atcggtaatt gcccgacgaa cggtaatcag actcgggtca ttcatatccg
     2281 ccaggctggc gtctgcactt ttcagcaacg ctgcagaggt cgtgacgtcc tgatcgctcc
     2341 acagcttccg tccggcgagt ttcaccagaa aatcggcctg agccagcagc caggttttag
     2401 catcgctgcc ggaaatggtg gcgatctttt gttggacttc atccaactgt tttgccagcg
     2461 tttcttgctg acgattcgcc tgcttaagct gcgcagcttg ttgcttaata atgccttcca
     2521 gctcggcttt ttggctctcc tgggcttttt gcaatgccgt cagttggtta gccagggcat
     2581 cgctggtggc ggtctgattg acggcctgtt gtttacccca gccatacaaa ccgacacccg
     2641 ccgccagagc aatagcgata gccaccgcgc tgagaatcaa tgcagtattg ttcttactct
     2701 ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc ctccctggtc tcttcaacca
     2761 cggcggaggt tttttcttgt tccgtcatta tggcttcctg ttatgagagt tattgtaatg
     2821 cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt aatgtcttgc cagcccagtt
     2881 cccgggcgag tttcgccaaa cgctcactga cgaccaatag tcgacagtgt agtaaccagt
     2941 gctcacgata ccattgtggg atcagcgacc agagttgctg caacatttca ccgctggtaa
     3001 caacgaccat cgtcacctcg cgggattgcc agcgcatcgc ttcttctgca ccatcgtaat
     3061 ggattgcgca tcgttgataa cattcacaaa aagtgacctc agcaccgcgc accgtcaggg
     3121 tatccccaat tagctcacga ccgccattgc cacgtaatat cagcgcacgt ttgcccgcaa
     3181 tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat ttcccgatcc tgtgggtaga
     3241 gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt ggtgcgtcca atggcgaaat
     3301 aagtaggtag ttggggccat ttacgatctt gctgatgcag ctgtgattgg gcaaaagcaa
     3361 ccgcgtgttg cgagagggca aacaacagat cgctctcccc cagcgctgcc agttgatcag
     3421 caagttgcgg taattgtcga cccggagaaa actcaatcag cggaaaatgc caggccacct
     3481 gccccagtgt gcgcagacgg ctcactaact cttctccagc gggagacggg cgggtgacca
     3541 ggatactcat gccggggcct ctccgttata gacttcagcg aggatctcgc gcgcgccgtt
     3601 attcagtagc tcttctgcca gcgaaatccc catttgttcg gcatcttgcg gcgcaccgcg
     3661 gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg accagcgcac atagccagat
     3721 ttcgccatca ataagctcgg cgtagctacc aattggcacc tgacatccgc cttcgagacg
     3781 ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt tcgtggtgat tcagcgcggc
     3841 aagcagctcg cgagtgcgtg tatcatcaag gcggcattca ataccgactg caccttgtcc
     3901 taccgccgga agagaaatct cgggtggtaa cgcggcgcga atgcgggact ccagacctaa
     3961 acgttttagt ccggctacgg caagaatgat ggcatcgtat tcgccgttat ctagtttgct
     4021 caggcgagtg ccgacgttgc cgcgcaggga gcggataatc agatccgggc ggcgttcagc
     4081 cagttggcac tggcgacgta aactggacgt cccgacgata ctgcctgctg gtaacgcatc
     4141 cagactgtca tagttattgg acacaaaggc atcgcgagga tcttcacgct cgcaaatagt
     4201 gaccagcccc agaccttgcg ggaattcaac cggcacatct ttcattgaat gtacggcgat
     4261 atcagcgcga ttctccagga gcgcgacttc cagctctttt acaaataagc cttttccgcc
     4321 tactttcgcc agcggcgtat caagaatcac atcgccgcgc gtcaccatcg gtaccagttc
     4381 aacgaccagg cccggatggc tcgccatcaa cttgtctttg acatagtgtg cctgccagag
     4441 tgcaagtggg ctttggcgtg tggcaattct taaaacattg tctaacatgc ttgttaccgt
     4501 cattatcatc cgtggtccat cctaacatcc ttgccagagt gatgtcagtg ttgtggtgaa
     4561 acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag tggatcctga caggcgtttc
     4621 acgccgtcgt aataaggaat ttacagagaa taaacggtgc tacacttgta tgtagcgcat
     4681 ctttctttac ggtcaatcag caaggtgtta aattgatcac gttttagacc attttttcgt
     4741 cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt tacctttgac atacgaaata
     4801 tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca cagtcatgac gggtagcaaa
     4861 tcaggcgata cgtcttgtac ctctatattg agactctgaa acagagactg gatgccataa
     4921 atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc tgcattccaa caggtctaca
     4981 gtctactgcc gacattgttg cactatcacc atccgcgaat gccgggttac cttgatggta
     5041 acgttcccaa aggcatttgc ctttacacgc ctgatgaaac tcaacgccac tacctgaacg
     5101 agcttgaact gtatcgtgga atgtcagtac aggatccacc gaaaggtgag cttccaatta
     5161 ctggtgtata caccatgggc agcacctcgt ccgtagggca aagttgttcc tctgacctgg
     5221 atatctgggt ctgtcatcaa tcctggctcg atagcgaaga gcgccagtta ctacaacgta
     5281 aatgcagcct gctggaaagc tgggccgcct cgctgggtgt ggaagtcagc ttcttcctga
     5341 ttgatgaaaa ccgcttccgt cataatgaaa gcggcagcct ggggggcgaa gattgtggct
     5401 ccacccagca tatactgctg cttgacgaat tttatcgtac tgccgtgcgt ctcgccggta
     5461 agcgtattct gtggaatatg gtgccgtgcg acgaagaaga gcattacgac gactatgtga
     5521 tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg gctggatctc ggtggcttaa
     5581 gctcgctttc tgctgaagag tactttggtg ccagcctttg gcagctctac aagagtatcg
     5641 attccccata caaagcggtg ctgaaaacac tgctgctgga agcctattcc tgggaatacc
     5701 cgaacccacg tctgctggcg aaagatatca aacagcgttt gcacgacggc gagattgtat
     5761 cgtttggcct cgatccatac tgcatgatgc tggagcgtgt tactgaatac ctgacggcga
     5821 ttgaagattt cacccgtctg gatttagtac gtcgctgctt ctatttaaaa gtgtgcgaaa
     5881 aactcagccg tgaacgcgcc tgtgtaggct ggcgtcgcgc agtgttgagc cagttagtga
     5941 gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga taaccgtgct aactggaaga
     6001 ttgatcaggt gcgtgaggcg cacaacgagc tgctcgacgc gatgatgcag agctaccgta
     6061 atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc cgccagtccg caggatatcg
     6121 gcgtactgac gcgtaaactg tatgccgcgt ttgaagcatt gccaggtaaa gtgacgctgg
     6181 taaacccgca gatttcaccc gatctctcgg aaccgaatct gacctttatt tatgtgccgc
     6241 cgggccgggc taaccgttca ggttggtatc tgtataaccg cgcgccaaat attgagtcga
     6301 tcatcagcca tcagccgctg gaatataacc gttacctgaa taaactggtg gcgtgggcat
     6361 ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat taaaggtaac ggcattgtcg
     6421 atttgcctaa gttgcaggag atggtcgccg acgtgtcgca ccatttcccg ctgcgcttac
     6481 ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat tcgccatctg gcgattatcg
     6541 ttaacctgga atatgacccg acagctgcgt tccgcaatca ggtggtgcat tttgatttcc
     6601 gtaagctgga tgtcttcagc tttggcgaga atcaaaattg cctggtaggt agcgttgacc
     6661 tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca cttcaacggc gagcaatcga
     6721 tgatcgaagc cctgaaaact attctcggca aaatgcatca ggacgccgca ccgccagata
     6781 gcgtggaagt cttctgttat agccagcatc tgcgcggctt aatccgtact cgcgtgcagc
     6841 aactggtttc tgagtgtatt gaactgcgcc tttccagcac ccgccaggaa accgggcgtt
     6901 tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt cttcgaacgc ctgaatgtat
     6961 cggtacagaa gctggagaac gccatcgagt tttatggcgc tatttcgcat aacaaactgc
     7021 acggcttgtc agtgcaggtt gaaaccaatc acgtcaaatt accggcggtg gtggacggct
     7081 ttgccagcga agggatcatc cagttctttt tcgaagaaac gcaagacgag aatggcttta
     7141 atatctacat tctcgacgaa agcaaccggg ttgaggtgta tcaccactgc gaaggcagca
     7201 aagaggagct ggtacgtgac gtcagtcgct tctactcgtc atcgcacgac cgcttcacct
     7261 acggctcaag cttcatcaac ttcaacctgc cgcagttcta tcagattgtg aaggttgatg
     7321 gtcgcgaaca ggtgattccg ttccgaacaa aatcgattgg caacctgccg cccgccaatc
     7381 aggatcacga tacgccgcta ttacagcagt atttttcgtg atgagcgtgc cggaaagcga
     7441 ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt cacccgcctg ttgcgtcgcc
     7501 gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat cgcaaatcca ttcatcgcct
     7561 ttcaggtcaa aatggtagcc gccctgtttg gttgccagcc acacctggtg cagcggctcc
     7621 tggcggttaa taatgatttt gctgccgttt tcaaaggtaa tggtcagtac gccgccgttg
     7681 atttcgcagt cgatatcgct gtcgccatcc ctgtcgtcca gacgctcttc aatggtcagc
     7741 cacagttgat cagccaggcg atgaaattca ctgtcgttca ttgttgtatc ctgtttttaa
     7801 gtgatggcgg cagtatagcg gcatggggtc agggcttcaa agtttgcacc tctgctgctg
     7861 cgttccggca tgattcatcc gtcaccggaa taatgatgtc tctgttttga acttcacgta
     7921 cgttcccctc tctgacacga gttttcgccg ggcaggggga gggcgtaatc cttacctctg
     7981 tatcggtcca tgtataaaca ttgcgatcgt cataagcgaa gcgatcgttt ttagttagcg
     8041 aaccgttaac tggaataatt tgcaagctct ttgaccgtaa ttggcggata aagtaagcat
     8101 gctgtttgcc gctattaaat gagcgatcaa tgaccaggtc tggcgtatcc agcgggatta
     8161 tctcaagctg gtcttcacca aatgtcgata attttgttac atacagccga tctttatatt
     8221 gcgcaatatt gccagtaatc agatggaatt
//
LOCUS       DAZPDE010000162         8163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPDE010000162 DAZPDE010000000
VERSION     DAZPDE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.165"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..982
                     /locus_tag="ACHU0J_003311"
     CDS             <1..982
                     /locus_tag="ACHU0J_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1217872.1"
                     /translation="DRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYD
                     FSHLSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDD
                     SNFAQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIA
                     PYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMV
                     ICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGE
                     ENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1136..2785)
                     /locus_tag="ACHU0J_003312"
     CDS             complement(1136..2785)
                     /locus_tag="ACHU0J_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1217873.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2936..4285)
                     /gene="ghxP"
                     /locus_tag="ACHU0J_003313"
                     /gene_synonym="yjcD"
     CDS             complement(2936..4285)
                     /gene="ghxP"
                     /locus_tag="ACHU0J_003313"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1217874.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4991..5455)
                     /gene="soxR"
                     /locus_tag="ACHU0J_003314"
     CDS             complement(4991..5455)
                     /gene="soxR"
                     /locus_tag="ACHU0J_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1217875.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5541..5864
                     /gene="soxS"
                     /locus_tag="ACHU0J_003315"
     CDS             5541..5864
                     /gene="soxS"
                     /locus_tag="ACHU0J_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1217876.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5867..7444)
                     /gene="pdeC"
                     /locus_tag="ACHU0J_003316"
                     /pseudo
     CDS             complement(5867..7444)
                     /gene="pdeC"
                     /locus_tag="ACHU0J_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7873..8154
                     /locus_tag="ACHU0J_003317"
     CDS             7873..8154
                     /locus_tag="ACHU0J_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR1217877.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2271 a         2089 c         1865 g         1938 t
ORIGIN      
        1 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
       61 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
      121 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
      181 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
      241 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
      301 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
      361 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
      421 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
      481 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
      541 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
      601 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
      661 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
      721 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
      781 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
      841 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
      901 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
      961 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
     1021 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
     1081 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
     1141 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
     1201 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
     1261 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
     1321 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
     1381 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
     1441 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
     1501 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
     1561 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
     1621 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
     1681 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
     1741 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
     1801 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
     1861 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
     1921 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
     1981 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
     2041 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
     2101 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
     2161 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
     2221 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
     2281 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
     2341 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
     2401 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
     2461 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
     2521 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
     2581 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
     2641 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
     2701 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
     2761 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
     2821 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
     2881 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
     2941 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
     3001 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
     3061 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
     3121 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
     3181 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
     3241 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
     3301 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
     3361 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
     3421 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
     3481 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
     3541 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
     3601 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
     3661 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
     3721 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
     3781 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
     3841 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
     3901 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
     3961 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
     4021 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
     4081 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
     4141 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
     4201 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
     4261 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
     4321 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
     4381 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
     4441 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
     4501 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
     4561 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
     4621 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
     4681 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
     4741 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
     4801 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
     4861 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
     4921 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
     4981 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
     5041 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
     5101 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
     5161 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
     5221 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
     5281 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
     5341 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
     5401 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
     5461 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
     5521 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
     5581 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
     5641 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
     5701 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
     5761 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
     5821 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
     5881 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
     5941 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
     6001 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
     6061 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
     6121 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
     6181 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
     6241 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
     6301 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
     6361 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
     6421 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
     6481 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
     6541 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
     6601 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
     6661 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
     6721 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
     6781 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
     6841 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
     6901 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
     6961 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
     7021 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
     7081 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
     7141 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
     7201 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
     7261 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
     7321 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
     7381 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
     7441 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
     7501 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
     7561 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
     7621 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
     7681 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
     7741 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
     7801 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
     7861 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
     7921 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
     7981 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
     8041 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
     8101 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
     8161 gaa
//
LOCUS       DAZPDE010000163         8036 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPDE010000163 DAZPDE010000000
VERSION     DAZPDE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.166"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            5..742
                     /gene="ygaZ"
                     /locus_tag="ACHU0J_003318"
     CDS             5..742
                     /gene="ygaZ"
                     /locus_tag="ACHU0J_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1217878.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            732..1067
                     /gene="ygaH"
                     /locus_tag="ACHU0J_003319"
     CDS             732..1067
                     /gene="ygaH"
                     /locus_tag="ACHU0J_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1217879.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            1158..1688
                     /gene="emrR"
                     /locus_tag="ACHU0J_003320"
     CDS             1158..1688
                     /gene="emrR"
                     /locus_tag="ACHU0J_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1217880.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            1815..2987
                     /gene="emrA"
                     /locus_tag="ACHU0J_003321"
                     /pseudo
     CDS             1815..2987
                     /gene="emrA"
                     /locus_tag="ACHU0J_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            3004..4542
                     /gene="emrB"
                     /locus_tag="ACHU0J_003322"
     CDS             3004..4542
                     /gene="emrB"
                     /locus_tag="ACHU0J_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1217881.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(4606..5121)
                     /gene="luxS"
                     /locus_tag="ACHU0J_003323"
     CDS             complement(4606..5121)
                     /gene="luxS"
                     /locus_tag="ACHU0J_003323"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1217882.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(5271..6827)
                     /gene="gshA"
                     /locus_tag="ACHU0J_003324"
     CDS             complement(5271..6827)
                     /gene="gshA"
                     /locus_tag="ACHU0J_003324"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1217883.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(6900..7328)
                     /locus_tag="ACHU0J_003325"
     CDS             complement(6900..7328)
                     /locus_tag="ACHU0J_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1217884.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(7325..7891)
                     /gene="yqaB"
                     /locus_tag="ACHU0J_003326"
     CDS             complement(7325..7891)
                     /gene="yqaB"
                     /locus_tag="ACHU0J_003326"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1217885.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         1891 a         2130 c         2020 g         1995 t
ORIGIN      
        1 ctctatggaa agccctactc cacagcctgc tcctggttcg gcgaccttca tggaaggatg
       61 caaagacagt ttaccgattg ttattagtta tattccggtg gcctttgcgt tcggtctgaa
      121 tgcgacccgt ctgggattct ctcctctcga aagcgttttt ttctcctgca tcatttatgc
      181 aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc gggagtagtt tgtgggttgc
      241 tgcactgagc gtcatggcaa tggatgttcg ccatgttttg tatggcccgt cactgcgtag
      301 ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta tgggcgtttg gcctgacgga
      361 tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat aatcgccgct ggagcgagaa
      421 ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg gtatttggta cggtaatagg
      481 ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc gttgaagctg cattaggttt
      541 tatgcttccg gcactcttta tgagtttcct gctcgcctct ttccagcgca aacaatctct
      601 ttgcgttacc gcagctttag ttggtgccct tgcaggcgta acgctatttt ctattcccgt
      661 cgccattctg gcaggcattg tctgtggctg cctcactgcg ttaatccagg cattctggca
      721 aggagcgccc gatgagctat gaggttctgc tgcttgggtt actggttggc gcggcgaatt
      781 attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa tgcccgccca accaaacgtg
      841 gcgcggtagg tattttgctc gacaccattg gcatcgcctc gatatgcgct ctgctggttg
      901 tctctaccgc accagaagtg atgcacgata cacgccgttt cgtgcccacg ctggtcggct
      961 tcgcggtact gggtgccagt ttctataaaa cacgcagcat tatcatccca acactgctta
     1021 gtgcgctggc ctatgggctc gcctggaaag tgatggcgat tatataaccc acaagaatca
     1081 tttttctaaa acaatacatt tactttattt gtcactgtcg ttactatatc ggctgaaatt
     1141 aatgaggtca tacccaaatg gatagttcgt ttacgcccat tgaacaaatg ctaaaatttc
     1201 gcgccagccg ccacgaagat tttccttatc aggagatcct tctgactcgt ctttgcatgc
     1261 acatgcaaag caagctgctg gagaaccgca ataaaatgct gaaggctcag gggattaacg
     1321 agacgttgtt tatggcgttg attacgctgg agtctcagga aaaccacagt attcagcctt
     1381 ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc gacgcgtatt gccgatgaac
     1441 tggaaaaacg cggttggatc gaacgtcgta aaagcgataa cgatcgccgc tgcctgcatc
     1501 tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt tttaccaccg cagcataact
     1561 gcctgcatca actctggtcc gcgctcagca caacagaaaa agatcagctc gagcaaatca
     1621 cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga cggtgtggtt ctcgaagcga
     1681 tgagctaacg cgtcatctcg ctcaaaaatc cagatttata aaagaaaaaa tgaccggcca
     1741 gcatcgcaac atgctggcct ttttggcaag caggtcggct cagccgatga gttaagaaga
     1801 tcgtggagaa caatatgagc gcaaatgcgg agactcaaac cccgcagcaa ccggtaaaga
     1861 agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt gctctttata attattgccg
     1921 tagcgatagg gatttattgg tttttggtac tgcgtcattt cgaagaaacc gatgacgcat
     1981 acgtggcagg gaatcagatt caaattatgt ctcaggtgtc tggcagcgtg acgaaagtct
     2041 gggccgataa caccgatttt gtaaaagaag gcgatgttct ggtcactctc gacccgacag
     2101 atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc ttccagcgtt cgccaaaccc
     2161 accagctgat gattaacagc aaacagttgc aggcgaatat tgaggtgcaa aaaatcgccc
     2221 tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct gggcaatgcc aacctgattg
     2281 gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag tgcccaggcg caactggacg
     2341 tcgctattca acaatacaat gccaatcagg cgatgattct ggggactaaa ctggaagatc
     2401 agcctgccgt gcaacaggct gccaccgaag tacgtaacgc ctagctggcg ctggagcgta
     2461 cccgtattgt cagtccgatg accggttatg tttcccgtcg tgccgtgcaa ccgggtgcgc
     2521 aaattagccc aacgacgccg ctgatggcgg tcgttccagc caccaatatg tgggtggatg
     2581 ccaactttaa agagacgcag attgccaata tgcgcatcgg tcagccggtc accatcacca
     2641 ccgatattta cggcgatgat gtgaaataca ccggtaaagt ggttggtctg gatatgggca
     2701 caggtagcgc gttctcactg cttccggcgc aaaatgcgac cggtaactgg atcaaagtcg
     2761 ttcagcgtct acctgtgcgt atcgaactgg accagaaaca gctggagcaa tacccgctgc
     2821 gcattggttt gtccacgctg gtgagcgtca ataccactaa ccgtgacggt caggtactgg
     2881 caaataaagt acgttcaaca ccggtcgcgg taagtaccgc gcgtgaaatc agcctggcac
     2941 ctgtcaataa actgatcgac gatatcgtaa aagctaacgc tggctaatcc agaggtgcgt
     3001 gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg tcattatgac gattgcgctg
     3061 tcactggcga cattcatgca ggtgctgaac tccaccattg ctaacgtggc gatccccact
     3121 atcgccggga atctgggctc atcgctcagc caaggaacgt gggtaatcac ttctttcggg
     3181 gtggcgaatg ccatctcgat cccgcttacc ggctggctgg caaagcgcgt cggggaagtg
     3241 aaactgttcc tttggtccac catcgccttt gctattgcgt cttgggcgtg tggtgtctcc
     3301 agcagcctga atatgctgat cttcttccgc gtgattcagg ggattgtcgc cgggccgttg
     3361 atcccgcttt cgcaaagtct attgctgaat aactacccgc cagccaaacg ctcgatcgcg
     3421 ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt gcggcccgat cctcggcggt
     3481 tatatcagcg ataattacca ctggggctgg atattcttca tcaacgtgcc gattggcgtg
     3541 gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg aaacccgcac cgaacggcgg
     3601 cggattgatg ccgtggggct ggcactgctg gttattggta tcggcagcct gcagattatg
     3661 ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg aaattatcat tcttaccgtg
     3721 gtggcggtgg tggctatctg cttcctgatt gtctgggagc tgaccgacga taacccgata
     3781 gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg gctgcttgtg tatcagcctc
     3841 gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc agttgttgca ggaggtctac
     3901 ggttacacgg cgacctgggc aggtttggcc tctgcgccgg tagggattat tccggtgatt
     3961 ctgtcgccga ttatcggccg cttcgcgcat aaactggata tgcggcggct ggtaaccttc
     4021 agctttatta tgtatgccgt ctgcttctac tggcgtgcct atacctttga accaggtatg
     4081 gatttttgcg cgtcggcctg gccgcagttt atccaggggt ttgcggtggt ctgcttcttt
     4141 atgccgctga ccaccattac gctgtctggt ttgccaccgg aacgactggc ggcggcatcg
     4201 agcctctcta actttacgcg aacgctggcg gggtctatcg gcacgtcgat aaccacgacc
     4261 atgtggacca accgcgagtc gatgcaccat gcgcagttga ctgagtcggt aaacccgttc
     4321 aacccgaatg cccaggcgat gtacagtcaa ctggaagggc ttgggatgac gcaacagcag
     4381 gcatcaggct ggattgccca gcagatcacc aatcaggggc tgattatttc cgccaatgag
     4441 atcttctgga tgtcagccgg gatattcctc gtactgctgg ggctggtgtg gtttgctaaa
     4501 ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact aagtacaact aagccagttc
     4561 atttgaactg gcttttttca attaattgtg aagatagttt actgactaga tgtgcagttc
     4621 ctgcaacttc tctttcggca gtgccagttc ttcgttgctg ttgatgcgta cctcacgttc
     4681 cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca
     4741 ctggtagacg ttcagttccg ggatctgatt ctgatcctgc actttcagca cgtcttccat
     4801 tgccgctttc caggcatcag caacacgctg ctcatctggc gtaccaatca gactcatata
     4861 aaaaccggtg cggcagccca ttggcgagat atcgataatc tctacaccat taccgttaag
     4921 atggttacgc ataaaaccag caaacaggtg ctccagggta tggatccctc tttctggcat
     4981 cacttctttg ttcggcacgc agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg
     5041 ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc atccgggtat gatcgactgt
     5101 gaagctatct aacaacggca tttagccacc tccggtaatt tttttaaaaa ttttctgaac
     5161 tctttcttcc caggcgagtc tgagtatatg aaagacgcgc atttgttatc atcatccctg
     5221 aattcagaga tgaaattttg gccactcccg agtggccttt ttcttttctg tcaggcgtgt
     5281 ttttccagcc acaccgcaaa cggttcggta tcagcggttt ccatttcctg ctgacggcgt
     5341 tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca gaatttccag cggctcttca
     5401 cgcagcagat tacggtaggc ttctgcaaac gctttgcctg ttccgccaat accagtatca
     5461 atcatagacc ttaagatacg ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc
     5521 agttcatcac acactttctg atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg
     5581 acgcgtttca gatcgcggaa cagatcttta cccacctgca gtaacgggaa ctgcgcggtt
     5641 tcgcagccga tacccagcgt caggcccggt ttgcgacctt caaggatcac ccggttccag
     5701 ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg gtgcatcagc cagcgcacac
     5761 cagaccataa acaggtcgag gaatcgcacc tgctgttcat ctacaccaat cggcgagaac
     5821 gggttgatgt ccagcgaacg cacttcaata tattcaatgc cgccacgtaa cagcgcatca
     5881 gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa tcggcgcata cagttcgttt
     5941 tcaatctgca gcacgttgct attgatttgc agcctcttac cgtctttctc aataccaatc
     6001 ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa
     6061 agatcgttga aggtaatacc aagattgctt tgcgatttat tggtatagcc gagatcgctc
     6121 aaacgaagag aggtcgcata cggcaggtaa tacataccgc actcggtttt ctcaaacggc
     6181 agcgacgttg gttttccttg caggaaagaa gaacaaatcg ccggagatgc accaaacaga
     6241 taaggaataa cccaaccgaa acgatagtaa ttgcggataa cgcggaaata gcccgcagaa
     6301 attttctctt tggcatcagc gcccgagata tcaccgcact tcgcttgcca gaatgccatt
     6361 ggcaaagaga aattgtagtg cacgccggaa atggtttgca tcagcgcgcc gtagcgattt
     6421 ttcagccctt cacgatacag cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc
     6481 agttcgatgt cctgaccttc tgcgatgtag catggcatac ttaacggcca catccgctca
     6541 tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca taaaggtcag catatgttca
     6601 atatcaccat ccactggtgt aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc
     6661 catttgtgcg tcagtgcgga acctaatgct tcaggatgac ctgttgttgc cagtgtgcca
     6721 tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct gtatcccctt taacgcctga
     6781 ggatgttttt ccagccaggc cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc
     6841 ctgtcaaaat cgttttaatt agcataactg taatggtgac catatgtgca ggcctacaat
     6901 tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct
     6961 ttaccaaggc acaaaaaaaa gattactggc ccccaggaga tgcgcatcca tcccgctaac
     7021 agacacagta aatcgccaac cacgggcatc cagcttaata atagcgtgac tgcaccatag
     7081 cgtttcagcc agccggtagc tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa
     7141 cgcccaagga taacgttagt taaccctcca aggctattac ccattgttgc tgttaaaact
     7201 aaaacccagg gatgactgat cccggaaagc aacattgcca ccagcacgac ttcggagttg
     7261 ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag cgataacgct
     7321 tcactcacag caagcgaaca tccacagcgt ccatgcctgc tgcacgggcc gcctgaatac
     7381 cgaaatcggc atcttcaaag accacacact gcgtcggttg cacgcccata cgctgcgcgc
     7441 acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg
     7501 cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc ctcagcgatg gcgctttcac
     7561 tccccgttcc tacagccatt gggcgacgac catgccaact tttcaccact tcaacaagag
     7621 gaagcggctc gacactatcc agcagcatac ttcttactgc ttctgttttt tcacgcgcta
     7681 acgcatgcgg gtcgagatcg gcctgattca gctcaataat tgcctgagca atacgccagg
     7741 tgggcgatcc attaagcgca atcatcgcct gaatatcgta ctgaagaccg tagtgcccta
     7801 atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc caggattgtg ccatccatat
     7861 caaaaattaa acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg
     7921 ctactttagc gtaattgctt gattttgtcg ctgatggcga aggagaagaa aattcaggaa
     7981 atggataaag tagttattta gaaatgagat atttttgaag gaaatttttt ggagaa
//
LOCUS       DAZPDE010000164         8009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPDE010000164 DAZPDE010000000
VERSION     DAZPDE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.167"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(356..431)
                     /locus_tag="ACHU0J_003327"
     tRNA            complement(356..431)
                     /locus_tag="ACHU0J_003327"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Asn,seq:gtt)
     gene            complement(532..2003)
                     /gene="yeeO"
                     /locus_tag="ACHU0J_003328"
                     /pseudo
     CDS             complement(532..2003)
                     /gene="yeeO"
                     /locus_tag="ACHU0J_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2164..2239
                     /locus_tag="ACHU0J_003329"
     tRNA            2164..2239
                     /locus_tag="ACHU0J_003329"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2197..2199,aa:Asn,seq:gtt)
     gene            complement(2277..3227)
                     /gene="cbl"
                     /locus_tag="ACHU0J_003330"
     CDS             complement(2277..3227)
                     /gene="cbl"
                     /locus_tag="ACHU0J_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1217886.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3329..4246)
                     /gene="nac"
                     /locus_tag="ACHU0J_003331"
     CDS             complement(3329..4246)
                     /gene="nac"
                     /locus_tag="ACHU0J_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1217887.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4574..4649
                     /locus_tag="ACHU0J_003332"
     tRNA            4574..4649
                     /locus_tag="ACHU0J_003332"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4607..4609,aa:Asn,seq:gtt)
     gene            complement(4705..5640)
                     /gene="ldtA"
                     /locus_tag="ACHU0J_003333"
     CDS             complement(4705..5640)
                     /gene="ldtA"
                     /locus_tag="ACHU0J_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1217888.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5702..6781)
                     /gene="cobT"
                     /locus_tag="ACHU0J_003334"
     CDS             complement(5702..6781)
                     /gene="cobT"
                     /locus_tag="ACHU0J_003334"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1217889.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6793..7536)
                     /gene="cobS"
                     /locus_tag="ACHU0J_003335"
     CDS             complement(6793..7536)
                     /gene="cobS"
                     /locus_tag="ACHU0J_003335"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1217890.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7533..>8009)
                     /gene="cobU"
                     /locus_tag="ACHU0J_003336"
     CDS             complement(7533..>8009)
                     /gene="cobU"
                     /locus_tag="ACHU0J_003336"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1217891.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2218 a         2143 c         1730 g         1918 t
ORIGIN      
        1 tccccgtccc cagagaaata atatttatta aaatggcagg tcttcttttt ctgattaccg
       61 aagacaaagt ggcaattacg tatagtttcc cgataaagac gcgacagcta cgtcccgcat
      121 aaggcatttt tctctttatc tttgtacggt acttcatgga acagaatttt tgaccttgtg
      181 aatcgtgata tctgttgggg aggggcaatt tgctcactga agcgtgagac tcgattaagc
      241 gcacgaaaca cagaaatcaa aaaacccggc cactttttta caaagtggcc gggtaaaaat
      301 aatttttatt ttttaactgt cctgagtctc atagagatgt ctcaaaacta aaatttggct
      361 cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga
      421 actacagagg aatcgtgaga acgaggcgaa tattagcgat gcccacccac aatgtcaaag
      481 cctgtttttt aaatttgaaa tcgtttgctg aaataatctg cattttgtcg tttattccga
      541 cacaactggc tttttttcac acttttgcgg ctcggatcga gggtatttcc acagccaacg
      601 tccagtaacc attcgccagt aaaacagcac ggcccgcaca gcccagtcgg caaacattcc
      661 catccagaca ccaaccacac cccagccaag catgattccc tgcacataac cgactacaac
      721 ccggcaaccc cacatgctca acatagaaac ccacatggcg taacgggcat cacgagcacc
      781 cttaaatcca gcgggtagca cccatgaggc ggaccaaata ggcataaata aagcatttag
      841 ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc tgggtgtaaa acgatgccat
      901 aaccccggca aagggagccg ttagccaggc gatggccgtt aatccaagag tggacagcca
      961 gaacacatgc cgcaactgaa tctctgcttg cgctatctgc cctaccccca accttcggcc
     1021 tgtaatgatc gtagaagcag aaccgagcgc acttccgggt aagttgataa gagccgcaat
     1081 tgagaacgcg ataaaatttc cggcaataac gctggtcccc atcccggcaa cgaacatttg
     1141 ggttaataac cgaccactgg taaataacac tgattcgaca ctcgctggaa taccaatccc
     1201 catgacttcc cagataatgc taaaattcag cggtttaaaa tagctcttta acgaaatcct
     1261 tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt gcaactgcgc caatataacg
     1321 agaaatggtt aaacccaacc cagccccgac caggagaaaa ggccgtaaat caatatgccg
     1381 ctaataataa tattaagaat attcaggcta ccgttaatca gtagcggtat tttcgtattc
     1441 cctgcaccac gaagtgcccc gctaccaata agagtgatgg cagcggctgg ataactgagt
     1501 acggtcagct ccagataagt caacgccagt gctttaactt ctgtcgtggc atcacccgcg
     1561 acgaaatcaa taatttgttc gccaaaatga tgaattagcg ttgccaacag tacggcaaac
     1621 aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg ctcgtcgtcg atcccgctta
     1681 ccgagactaa atgctacaac gacagtagta ccaagatcga tagctgcaaa aaaagccata
     1741 atgaccatat tgaagctgtc cgccaacccc acgccggcca tcgcatcttt tcccagccaa
     1801 ctgaccagaa aagtgctcag gacccccatc aacaggacac aggcgttctc catgaagata
     1861 ggaacagcaa gcggggttat ctcgcgctag aacaacactt tgtagctctt gcgtttagag
     1921 tgccagcgag tgccgtgaac aacctggcgt aaagcagagg agatattcaa agccgacctt
     1981 aattgcagaa agtgaaacca catttcaaat gatgagggag aatcagcaaa gctgcaaaga
     2041 ttttcgccaa caaattgtct gcaaatgcaa caaactgttg atagaaacgg caaacagttg
     2101 ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg ttaagatgtg cctcaacagc
     2161 gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt cactggttcg
     2221 agtccagtca gaggagccaa atttctgaaa agcccgcttt tatagcgggc tttttgctat
     2281 atctgataat caatttcctc ttcactgttt tccatcacct ggcgcttgat atcctcaact
     2341 gacagtcctg cattacaaag ttccagaaag cgccaaacat agttacgctg aagttgtcct
     2401 cgcttcagtc ccaaccagac agtattagca tcgaaaagat gccgcgtatc caggcggatt
     2461 aaattctctt cctcttgttc gccgctggat tgttcggcaa ctaatccgat cccaagccca
     2521 agagcaacat aggttttaat gacatcagaa tcctgtgcac ttaatacaat atctgccggc
     2581 aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc ccgtaattcc ctgtcggtaa
     2641 gtaattaacg gccacttcgc tattgattcc agcgtcaatg gtgtaatttg cgttaaggga
     2701 tgatcgagtg gaacaagcaa actatggtgc caacaaaacc atgggaaggc gacgagctgc
     2761 gggtcattac tcaaacgctc gctggcgata ccaatatcag cttcgccatt ttgcaacaac
     2821 gtcgcaattt cctgtggcga accctggatt agctcgagcc gaacctccgg gaaaagttcg
     2881 cgaaaagctt taatgacctc tggcaagcta taacgtgcct gagtatgcgt cgttgcaata
     2941 gtgagaacac cagacgtatc gttggtaaac aggtctgcaa gccgacgaac attactggct
     3001 tcattcagaa tacgttctgc aatgaccagt aatgctttgc ccggttcagt catccccagc
     3061 agtcgcttac ctcgtcgaac aaatatttcg atgccaaatt catcctccag ttcccgaata
     3121 tgacggctaa cgcctgactg tgaggtaaaa agcatattcg caacctctgt caggttgtaa
     3181 tcctgacgtg cagcctcgcg gattatcttt agttgttgga aattcacggt aaactccggg
     3241 cagttcagat atcccgttat tgttaaagtc taatgcccgg cataacaaat aataaaaacc
     3301 cgcatcttat tccatcccga tataacgctt agctcaccaa ttgccactgc cttttttcca
     3361 tcactggaga actaatcact gacattaaca actctttcac tgcctgtgcc tgtggtgaca
     3421 agtttgctct ggcgggtaaa tttaaggaca aagagagact cattgaagga gtggtaatgc
     3481 gtgacatcca cccatttact gcgccacata acgaacgcgc agccgattca ggtaatactg
     3541 caacgcccat gccgctggca atcgctgcgg taagcgtggc aatagactca atttcaccaa
     3601 tgacttttgc cgtgagccgc cgtagggaaa aagcctcatc aacacgaagt ctaacagcgc
     3661 tgtaatcact ggggagaaaa aggttcattt gcgcaatagc attaacatca acgttttgcc
     3721 ccgggcaatc ttgagttcct accaggaaaa gatcttcttt cagcaaagcc tgactggata
     3781 ccccagccac aggggaatgc tcataaatca ccgccatatc gagttggtga tttatcagtt
     3841 tttcgttaag cactgcacca ctattttcat gaagatagat aacgacctcc gggaattcag
     3901 cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc agcggttcct ggcgcaaagc
     3961 caatcgagac ttgccccgat aatgactgac caacgttatg caccgccagt tgggcctgct
     4021 cacactgacg taaaatggcc cgtgcatggg tatagagaat ttttccggcg tctgttggcg
     4081 taacgccccg ttttgtgcga atcaaaagct gttgatttaa ctcaccttcc agtatggcaa
     4141 cctgttggct gagcgctggt tgtgcgatat gcaatacttc agcagcctgg gtcaggctac
     4201 caatatctac aatttttacg aagtatttca ggcgtctgaa gttcatgttg cctccggttt
     4261 ttaagaatcg gcccaagtgc cgccattact tacaaccagt atgcaagatg cctgccagtt
     4321 ctattttggc gttgatgcgc aagctaacca actgtcaaat aagagattat gatagattcg
     4381 tcattggccc tttttatcag cagtcgcatt cacctgccct ataaaaggag ggtatgcacc
     4441 acgatggttc attacccaat aagattgaaa gctcaccact ttgttgaaat tggcagcaaa
     4501 caaacaaaaa aaatgcattt caccctttga catcaccatg cactgccatt aatatgcgcc
     4561 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     4621 cactggttcg agtccagtca gaggagccaa attcaaaaaa gcctgctttc gagcaggctt
     4681 tttactttct aattaccaac gctcttaaaa catctgtctt gaaccagaac taatttgcac
     4741 aggcattccc gatcgatgtt gcaacgcagc atttgcgcga tttacatcaa cttcttgccc
     4801 gttgataaac gcccgcaaag atggggttac cggcaatggc acttttcggt cagactcata
     4861 ttctgcacga ttgcgcgaca gcggctcatg aacttccagc cactttgaac catccggttc
     4921 cgttgtgtat ttcactggct gatcaataat ttgtacacga gtgcctacag gaacattatc
     4981 aaacagatat ttgatatcgt cattacgcag acgaatacag ccctgactta cacggagccc
     5041 aataccaaaa ttggcattgg taccgtggat ggcatacaac ctgccaatat aaatcgcgta
     5101 cagccccatg gggttatcgg gccccgcagg aacaaatgcg ggcaaactct cccctcgttt
     5161 cgcatattcg cgccggatgt tcggcgttgg cgtccaggtt ggtgcttctt gtttacgttc
     5221 aacggtagtc acccagttac gcggggtttc tcgcccagcc tggccgatac caataggaaa
     5281 gacttccact gtattactgt ctggcggata gtaataaaga cgcatttcag cgacgttaac
     5341 cacaatccct ttacgaacag tggcgggcaa aatcagttgc tgcggaatgg tgagttgtga
     5401 gccaggcttc ggcaaaaaaa catcagcgcc cggattcgct tccagcatat tacttaaccc
     5461 ttgcccgtat tgtgcggcaa aagtctccag cggctgagta ttgtgatcag gaacagttac
     5521 agtaagcgac tgccccacta aacggctacc ctctggaggt aatggataag ttaccgccag
     5581 gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga atatttacac gacgcatcat
     5641 gtccctttcc taagtcgcga atactatccg ataagtatag cttttatcag acttttcgtt
     5701 tttagctgtt caaatcagaa gtcgtattcc ccggtagaac aatattactg gcagcaagtt
     5761 cgcccatgtt gttgtatatc gcacaagcag cttcgatgat ggacatcgcc agagctgcac
     5821 cactcccctc acctaaacgc atatccatat tgagataagg ctccagcccc aaatgcgaga
     5881 gcgctatacg cgcgcctttt tctgccgaca agtgagaagg aatgagatac ggtttgattg
     5941 caggagacat ctggcaggct gcgagcgcag cagcataaga aagaaatcca tccagcaaca
     6001 cgggtaaacc gcaggaagca gcacctaaca tcactccagc tattccgacc aaatcaaatc
     6061 cacccacttt tgccaggaca tcaacaccat cctgaggatt tggttgattc aacgtaatcg
     6121 cccgacgcac aacatcaatt ttattagcca gtttatctgt cggcaggttt gcgccaatcc
     6181 caaccacttc ttcaggatcc cggccagtga ttgtgctgac tattgccgct gccggtgtcg
     6241 tgtttgccat ccccagttca cctacaccaa acagcgtgac accgtttttc gccagctccc
     6301 gcgtataaca tatgacgtcc aaaagcaact tttcagcctg acggcgactc attgccggag
     6361 ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt gataagcccg gggataggct
     6421 cagcagtatc aataccaaca tcaattacgt ggacgttagc gcccgcttgt gctgccagca
     6481 cacacacgcc ggttgttcca cgggtcatat tttcagcctg tatggctgtc acttcttttg
     6541 gagaaatagc gaccccttcc tcccagacgc cgtgatcggc acacataacc agtaccgctt
     6601 ttttgcccac atgcggtatg ccattcaccc ccggcattcc tgccagttgt atggcaagcg
     6661 cctccagctt tcctaggcta ccaacaggtt tgaataaccc gtcaacatgc cgttgtgcac
     6721 gcgacatagc ggcagaattg atggctggga tcgtattcag taaatcggca attatttgca
     6781 tgtcacgttt ctttatagca gtgccagcag gaagaccagt tcaccaagct ctatggctgc
     6841 tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg agaagctgac cgaggatgaa
     6901 aatcgccacc atcgtcacca ccatagcagc cacaccatgc atacccagca ataatacggc
     6961 agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt cgcccatcaa ttttgccaat
     7021 gaacacattg ccaagccctt cttcccgcgc ataacgatga cgatacatca ataatgcggc
     7081 agtcccgcgg ctgaccgcac atgctgccgc cagcgatgca aggatcggtt cgccacgcag
     7141 agccaactcg cttaacacca gaatctttgc cagtaccaca aaaatcaatg ccagaccgcc
     7201 gtgggtgcct aaacggctat cacgcatgat ttccaacatg cgatcgcgac tacgtgcaga
     7261 aaatacgcca tcacaggtat cagcgaggcc gtccagatgg aattccccgg tcatcagcgc
     7321 cagcacaagt acgctaaaca gtgccgccag tggtgcgcca caccatgcct gcagcaccat
     7381 gaagaccagc ccgctaatcg cgccaagcaa taatccaatc aaaggaaaag taataatacc
     7441 gcgagaataa tgttcgaaat ccagtccctg ggaccagcga cgcggtacag gcagacgcgt
     7501 aataaatgag agcatcgccc aaaataattt gctcatttga tttttactcc aatacccgaa
     7561 accaccagcc atacttcatt tgccgcagcg gccaactgct gatttacccg cccggcaata
     7621 tcacgaaaat gtcgtgccag acgactctcc ggcacaatcc ccattcccac ttcgttagtc
     7681 actaatacaa cctttgcggg gcaacgttgg caggcagcaa tcaacgactg aatctcagca
     7741 ttaatcgcct gttccatcgc ctgataatcc cattcatcag ggtctttatc gccgccataa
     7801 tcaaacaaca gattagtcac cattgtggta acgcattcca gcaacacagc ctcattcggg
     7861 ttaatgtctg catgaattaa ttcatcaaga tgttgccagc gctccactgt gcgccagtgc
     7921 tccgggcggc tttgccgatg atgttctatc cgtgcagcca tctcatcatc aaggatttgc
     7981 gaggtagcga tatacagaac ctgtgaaga
//
LOCUS       DAZPDE010000165         7913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPDE010000165 DAZPDE010000000
VERSION     DAZPDE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.168"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHU0J_003337"
     CDS             complement(<1..956)
                     /locus_tag="ACHU0J_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR1217892.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHU0J_003338"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHU0J_003338"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR1217893.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHU0J_003339"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHU0J_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHU0J_003340"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHU0J_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1217894.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHU0J_003341"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHU0J_003341"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1217895.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHU0J_003342"
     CDS             complement(5018..6346)
                     /locus_tag="ACHU0J_003342"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1217896.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..7913)
                     /locus_tag="ACHU0J_003343"
                     /pseudo
     CDS             complement(6354..7913)
                     /locus_tag="ACHU0J_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         2100 a         2005 c         1783 g         2025 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt att
//
LOCUS       DAZPDE010000166         7909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPDE010000166 DAZPDE010000000
VERSION     DAZPDE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.169"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(123..953)
                     /locus_tag="ACHU0J_003344"
     CDS             complement(123..953)
                     /locus_tag="ACHU0J_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR1217897.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="ACHU0J_003345"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="ACHU0J_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1217898.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="ACHU0J_003346"
                     /pseudo
     CDS             2379..2729
                     /locus_tag="ACHU0J_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            2855..3415
                     /locus_tag="ACHU0J_003347"
     CDS             2855..3415
                     /locus_tag="ACHU0J_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1217899.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="ACHU0J_003348"
     CDS             3418..6384
                     /locus_tag="ACHU0J_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1217900.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="ACHU0J_003349"
     CDS             6451..6828
                     /locus_tag="ACHU0J_003349"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1217901.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="ACHU0J_003350"
     CDS             complement(7029..7688)
                     /locus_tag="ACHU0J_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1217902.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAZPDE010000167         7864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPDE010000167 DAZPDE010000000
VERSION     DAZPDE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.170"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            183..686
                     /gene="dps"
                     /locus_tag="ACHU0J_003351"
                     /gene_synonym="pexB"
     CDS             183..686
                     /gene="dps"
                     /locus_tag="ACHU0J_003351"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1217903.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1090..1836
                     /gene="glnH"
                     /locus_tag="ACHU0J_003352"
     CDS             1090..1836
                     /gene="glnH"
                     /locus_tag="ACHU0J_003352"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1217904.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSRIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            1975..2634
                     /gene="glnP"
                     /locus_tag="ACHU0J_003353"
     CDS             1975..2634
                     /gene="glnP"
                     /locus_tag="ACHU0J_003353"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1217905.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2631..3353
                     /gene="glnQ"
                     /locus_tag="ACHU0J_003354"
     CDS             2631..3353
                     /gene="glnQ"
                     /locus_tag="ACHU0J_003354"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1217906.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3614..5838
                     /gene="ybiO"
                     /locus_tag="ACHU0J_003355"
                     /pseudo
     CDS             3614..5838
                     /gene="ybiO"
                     /locus_tag="ACHU0J_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5835..6761)
                     /gene="rlmF"
                     /locus_tag="ACHU0J_003356"
     CDS             complement(5835..6761)
                     /gene="rlmF"
                     /locus_tag="ACHU0J_003356"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1217907.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7037..7297
                     /gene="mcbA"
                     /locus_tag="ACHU0J_003357"
     CDS             7037..7297
                     /gene="mcbA"
                     /locus_tag="ACHU0J_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1217908.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7562..>7864
                     /locus_tag="ACHU0J_003358"
     CDS             7562..>7864
                     /locus_tag="ACHU0J_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1217909.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         1915 a         2014 c         1971 g         1964 t
ORIGIN      
        1 agcagataaa taagaattgt tcttatcaat atatctaact cattgaatct ttattagttt
       61 tgtttttcat gcttgttacc actattagtg tgataggaac agccagaata gcggaacaca
      121 tagccggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga ggatatgaaa
      181 ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat acccgcaacg
      241 atgtctccga cagcgagaaa aaagcaacag tagagttgct gaatcgccag gttatccagt
      301 ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc gctaacttca
      361 ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact gatcgatcat ctggatacca
      421 tggcagaacg tgccgtgcag ctgggcggtg tagctctggg gaccactcaa gttatcaaca
      481 gcaaaacccc gctgaaaagt tacccgctgg acatccacaa cgttcaggat cacctgaaag
      541 aactggctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga
      601 aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat aaattcctgt
      661 ggtttatcga gtctaacatc gaataaatcc atcgctgatg gtgcagaact ttagtacccg
      721 ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca gcccacctca acgcacttat
      781 ttagtgcatc catctgctat ccccagctga ttaagtaaat tttttgtatc cacatcatca
      841 cacaatcgtt acataaagat tgttttttca tcaggtttta cgctaaataa tcactgtgtt
      901 gagtgcacaa ttttagcgca ccagattggt gccccagaat ggtgcatctt cagggtattg
      961 ccctataaat cgtgcatcac gtttttgccg catctcgaaa aatcaatgag ttgcaaaatt
     1021 ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa
     1081 ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct ggcttttgcg
     1141 gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc cttcgttccg
     1201 tttgaattta aacagggcga taaatatgtg ggctttgacg ttgatctgtg ggctgccatc
     1261 gctaaagagc tgaagctgga ttacgaactg aagccgatgg atttcagtag gatcattccg
     1321 gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac cgacgagcgt
     1381 aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt gatggtgaaa
     1441 gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt tgctgtgaag
     1501 agcggtactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag
     1561 ttcccgaaca tcgataacgc ctatatggaa ctgggcacca accgcgcaga cgccgttctg
     1621 cacgatacgc caaacattct gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg
     1681 gtaggtgact ctctggaagc gcagcaatac ggtattgcgt tcccgaaagg tagcgacgag
     1741 ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa
     1801 atctacaaaa aatggttcag tactgaaccg aaataataac gctacacctg taaagcgcac
     1861 tggcagttcc ctctcccccc tggggagagg attagggtga ggggcgcaaa cccgctccgg
     1921 ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca acatatgcag
     1981 tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc
     2041 ctgtggattt cggtcctcgg tctggctggc ggtctggtaa tcggattgct ggcaggtttt
     2101 gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat tgaagtgatc
     2161 cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt tcgccctgcc gatggcgttt
     2221 aacgacttac gcatcgaccc atttactgcg gcggtggtca ccatcatgat caactccggc
     2281 gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta tccacaaagg ttttcgtgaa
     2341 gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat tttaccgctg
     2401 gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagcat taaagacacc
     2461 tcgctgttta ttgttatcgg cgtggcggaa ctgacccgtc aggggcaaga aattattgcc
     2521 ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct gattattacc
     2581 ctggtgctga gctttattct gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat
     2641 ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct gcacaatatc gatttgaaca
     2701 ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc
     2761 tgcgctgcat caacaaactg gaagaaatca cctccggcga tctgattgtc gatggcctga
     2821 aggtcaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt atggtgttcc
     2881 agcagtttta cctcttcccg catctgacgg cgctgggaaa cgtcatgttt ggtccgctac
     2941 gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag
     3001 tcggtctggc agaacgtgca catcactacc cttccgaact ttctggtggt caacagcagc
     3061 gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat gatgctgttt gatgaaccga
     3121 cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag gatctggctg
     3181 aagaagggat gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag aaagtagctt
     3241 cgcggctgat ctttatcgac aaaggccgga ttgcggaaga tggcaatccg caggtgttga
     3301 tcaagaaccc gccgagccag cgcttgcagg aatttttgca gcacgtctct taataagaca
     3361 cattgcctga tgcgctacgc ttatcaggcc tacaggatat ctggcaactt attaaaattg
     3421 catgaacttg taggacggat aaggcgttca cgccgcatcc ggcaaaaaag cccgcacgtt
     3481 gtcagcaacc tgcttaatat cccttcctcc ctttcacccg aaagggaggc acaccagatt
     3541 cctctcattt aaaatcgccc ctcccccagc atctatactt atctttttgc tctgttttct
     3601 cactggagga gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg gcgcacctgc
     3661 ccacgcggta tccatacccg gcgttacaac cacaacgaca acggactcaa cgactgaacc
     3721 ggccccggaa ccggatatcg aacaaaaaaa gcggcctatg gcgcactggc ggatgtgctg
     3781 gataacgaca cctcgcgtaa agagttgatc gaccagttgc gtactgtcgc cgcgacgccc
     3841 cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca aaccgtgctg
     3901 caaaaggtca ccgaagtcag ccgccattat ggtgaagccc tttccgcccg cttcgggcaa
     3961 ctttatcgca atatcaccgg ctccccgcat aagccattta atccacaaac cttcagcaat
     4021 gcgctgagcc atttttcaat gttagcggta ttagtgtttg gtttttactg gctgattcgc
     4081 ctgtgcgcac tgccgctata tcgcaaaatg ggtcagtggg cgaggcaaaa aaatcgtgag
     4141 cgcagcaact ggttgcagct tccggcgatg attattgggg cgtttattat cgacctgctg
     4201 ttactggccc tgacattatt tgtcggccag gtattaagcg acaacctgaa tgcaggcagt
     4261 cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct ttgccctgat tgaatttttc
     4321 aaagccgtac tacgcctgat tttttgccca aacgtggcgg agctgcgccc gttcacgatt
     4381 caggacgaga gcgcccgtta ctggggtcgt cgcctgagct ggttaagcag cctgattggc
     4441 tatggcctga ttgtggccgt gccgattatc tctaatcagg tgaatgtaca gataggtgcg
     4501 ctggcgaacg tcatcattat gctgtgcatg accgtctggg cgttgtacct gatcttccga
     4561 aataaaaaag agattaccca gcatatgctc aactttgcgg agcattcgct ggcttttttc
     4621 agcctgttta tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc
     4681 atgctgtttt tcttttcgtt gttcgatccg ggcaacagtc tgaaatttat gatgggtgca
     4741 acggtgcgca gcctggcgat tattggtatc gcagcgtttg tttccggtat gttttcccgc
     4801 tggctggcga aaaccatcac cctctcgcca catactcagc gtaactatcc ggagctacaa
     4861 aaacggctga atggctggct ttcggcggcg ctgaaaacgg cgcgtattct gaccgtctgc
     4921 gtggcagtaa tgttgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa
     4981 aacggcgcgg ggcagaaaac cgtagatatc ctgatccgca tcgcactcat tcttttcttc
     5041 tcggcggttg gctggacggt gctcgccagt ttgatcgaaa accggctggc ttcggatatt
     5101 catggccgcc cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt ccgtaacgcg
     5161 ctggcggtga ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgtcaat
     5221 atcgcgccat tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa
     5281 acgctggtaa aagatattat caccggggta tttattcagt ttgaaaacgg catgaacact
     5341 ggagatttgg tgactatcgg gccgttgacc ggcacagtgg aacggatgtc gattcgctcc
     5401 gtgggcgtgc gccaggatac cggggcgtat cacatcattc cgtggtcttc gataaccacc
     5461 tttgctaact tcgtccgcgg tattggttcg gtagtggcaa actacgatgt tgatcgccat
     5521 gaagatgctg ataaagccaa tcaggcactg aaagatgcgg taacggaatt gatggaaaac
     5581 gaagaaattc gcgggctgat tattggtgaa ccgaactttg ccgggattgt cggcttaagc
     5641 aataccgcgt ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggta
     5701 cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt tcgcgcgcca
     5761 gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc cggctgaacc gctgccgccg
     5821 ggggagccaa cgctttaacg ttggcgatta acaaaacggc ggcgctgctc gtcgttcata
     5881 aaggtccagg caataaagcg actctgcttt tgcccctggg ccatctcttt ttttaccacc
     5941 ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg gtaagttttc accacgagat
     6001 accagcgagg taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt
     6061 ttgataaagt tgacttcacc gccttcacac cacaactcct gttgctgacc accaaagttc
     6121 agtgcatcgt ctttgttcag ccccaggtta cggcgtttac gctcactacc tgcccgcgcc
     6181 gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg ctcgtttttg
     6241 tggatgatgc cgttaaaaat cgccccactc tctttttgcc gacgcagacg aatggcgcgg
     6301 ttaagccccg gattagcact gataatcgcc tgcgcactgc ttaacgcctg gctgctggtt
     6361 tcactaccgg taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc
     6421 gcaccaacgc cgatatccag aatgctggca ttcgctggaa ttgttccgct tgcttcagcc
     6481 agtaaatcgg caaggtgatg aatataatcc gcccggcccg gtactggtgg gcagagaaaa
     6541 ccgtcgggga tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc
     6601 gccttcaccg ccagcggatt ggcaaagtct acgctttgct cccctgcggg tgtaagcgtg
     6661 aggaattgcc tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga
     6721 tgacggttgc gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag
     6781 cggcgtaaga tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcattcccc
     6841 aaaaccttaa catggctatg gtttatggca attcatcata aaaatcagca ttatgcatgt
     6901 cattgatttg tcttattttg taaaaatata tcgttcaaaa aataagcatt tctctgtcaa
     6961 ttttttgcgt catttgcatc gcgcaggcga cctacactta tcaggcacta cccgttgtat
     7021 ttatgaggat gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcgg
     7081 aatctctctc acggcttatg ccgcgcaacc gatgagtaac ctggacagcg gtcagctacg
     7141 gcccgccggt accgtttcgg caactggcgc atcaaaccta agcgatctgg aggataaact
     7201 ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg caggcggaaa
     7261 tgaccagatg ttaggtactg caaccatcta caaataaccc caccaacgta actgcattcg
     7321 cctctggatg cagttttcgc ctgttttgta atcaaaaaac atcaaatatg aacgcaatgt
     7381 aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa atcgcatcaa gtgataatgc
     7441 ttatcaaaat tattatcact tgcacgaaca ctatcacggg attaacagtg gcatcgcatc
     7501 cgcagagatg ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag tcacctgcaa
     7561 aatggaaaac aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg
     7621 tctttgtatt ggtattacgc ctgtggcaca ggcactcgcc gccgaagggc aagctaacgc
     7681 ggatgacacg ctggttgtcg aagcatcaac gccttcgctt tatgcgccac aacaatctgc
     7741 cgatccgaaa ttctcgcgtc cggtagcgga tactacccgc acgatgacgg tgatttctga
     7801 acaagtgatt aaagatcagg gcgcaaccaa ccttaccgac gcgctcaaaa acgtccccgg
     7861 cgtg
//
LOCUS       DAZPDE010000168         7806 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPDE010000168 DAZPDE010000000
VERSION     DAZPDE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.171"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(164..649)
                     /gene="sixA"
                     /locus_tag="ACHU0J_003359"
     CDS             complement(164..649)
                     /gene="sixA"
                     /locus_tag="ACHU0J_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1217910.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(852..2996)
                     /gene="fadJ"
                     /locus_tag="ACHU0J_003360"
     CDS             complement(852..2996)
                     /gene="fadJ"
                     /locus_tag="ACHU0J_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1217911.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2996..4306)
                     /gene="fadI"
                     /locus_tag="ACHU0J_003361"
     CDS             complement(2996..4306)
                     /gene="fadI"
                     /locus_tag="ACHU0J_003361"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1217912.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4486..4770)
                     /locus_tag="ACHU0J_003362"
     CDS             complement(4486..4770)
                     /locus_tag="ACHU0J_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1217913.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5142..6482
                     /gene="fadL"
                     /locus_tag="ACHU0J_003363"
     CDS             5142..6482
                     /gene="fadL"
                     /locus_tag="ACHU0J_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1217914.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6848..>7806
                     /gene="yfdF"
                     /locus_tag="ACHU0J_003364"
     CDS             6848..>7806
                     /gene="yfdF"
                     /locus_tag="ACHU0J_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR1217915.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKKGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNL"
BASE COUNT         2081 a         1992 c         1790 g         1943 t
ORIGIN      
        1 aaaatcaaga tacgcgtaga agaataattt ccgaatgaat gtagaaagtg ttttatttat
       61 agataaggag agtcttcgat agtagtgagg ttgacagata tccgctgcct aatgagcaat
      121 tatgcccaat gaatctacct cattgggcat aatttggaac acatcagata gcttttgcca
      181 tcttcagatt acacgggctc atctgccagt tgaatgtacc gtttccgctt tcatcaagcg
      241 ttacgctggc aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg
      301 caaccagata cccgactaac ggtaagtgag agatcaccag taccgaagca acaccttcat
      361 tggtcaacgc ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg
      421 gcagaacttc tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct
      481 cggctcgcag gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc
      541 agttcgccat caggcgagat tcgtcacaac cattagtggt cagaggacga accgaatcac
      601 tggcggaatc gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct
      661 tttgttaacc agattcgccc gttttctttc atctgaaacc agagaaagaa aacggtggcc
      721 ggcattgtgc cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt
      781 atagtcaata tgcgtttaca ttttgaccaa cactccgcca ttcagcgcgg attcatatag
      841 ctttgacctt cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcgcg
      901 cccatctcga ccaaacgctc gcaaggggta aaacgggaac catactgcgt ggccagtcgt
      961 tgcattattg caaccacttc gcccgcgccg agagaatcga tatagcggaa cggtccaccg
     1021 agaaatggcg gaaaaccaat gccaaatacc gcaccaatat ccccgtcacg cacgctacgg
     1081 ataacctgct catcaacaca acgtactgct tcattcagca tcaacatcac acaccgttca
     1141 gcaacctgcg gtgcggagag tcgcccctgc ccttgtgcgc caatgagcgg gtaaatggca
     1201 ggatcgacct gttttttgct tttacgccct ttctgaccat aaagatagaa accccgacca
     1261 ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta
     1321 aaacgttctc cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct
     1381 acctcatcca aaagttggat tgggcctacc ggaaaaccaa atttcactag cgcggcatca
     1441 atgtgctcta cccgttcacc ttcggtcagc atgcggatag cttcattaat gtaaggcgct
     1501 aagatgcgat tgacgtaaaa cccggctttg tcacgcacga caatgggcgt tttgccctgt
     1561 tttttcgcca gttttaccgt agtggcgatg gtttgcgccg atgtacccgc atgaggaata
     1621 atctctacca gcggcatttt ttccaccgga ctgaagaaat gcaggccaat aacttgctca
     1681 ggtcgcgcgg cgtgagcggc gatatcacca atcggtaaag atgacgtgtt cgaagcaaaa
     1741 atggtatgag cggcgcaatt ttgctccact tccgccacca tctgttgttt taattcgaga
     1801 ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt
     1861 ccggagatta atgccagctg tttgtcacgt tcgctggctt tgagatgacg acggcgaact
     1921 ttgccctcca gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata
     1981 tctttaattc tgaccggaag ccccgcttta caagcagtga cataagcaat accgccgccc
     2041 atcaagccac cacctaaaat tcccacgctg tttaatggcg caggcggtgc atcactaccg
     2101 ggatctttct tcacgtccgt actggcaaaa aagatactac gcaaagcttg cgattgcggc
     2161 gtcatcgcca gttcgccaaa ggcgcgagct tcagcgtcat agccgctgct ggtgccctgc
     2221 gctaatcccg tttcaacaac ctccaggatg cgttctgtcg ccggataatt gccttgagtt
     2281 ttgtgttctg ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc
     2341 agaatacgct cgcgtacagg tagagggcgg gaagatgggc gatccttctt cgccagctca
     2401 acagcggctt ccagcaaaat ggagtgcgga acaacgtcat ccaccagccc cagctttaat
     2461 gcctgtttcg cccgaagttg ttttccggtg agaatcatct ctaatgctgt actgacgcct
     2521 atcagacgcg gtaaacgctg ggtgccgcct gaaccgggta acaatccaag ttgtacttca
     2581 ggcaaaccga gcaccgtttt aggatcgtca gtacaaacgc gaccgtggca cgccagcgcc
     2641 agctccagcc caccgcccag gcaagcgcca tgaatagccg cgataaccgg aatgggcaaa
     2701 gcatgaatct ccgccatcaa ctgttggccc tgccgcgcca gtgcttccgc ttcttgcgcc
     2761 gttttgcagt tgccaatcat gttgatgtct gctccggcga taaaattgtc cggtttcgcg
     2821 gagataaaca ccacgccgcg caactctttg ttttcacgga gttgcttaat aatggcgcgc
     2881 acctgcgagg caaattccgc cttcagggta ttcattttct catccggtac gtcgatggtg
     2941 atgatagcaa tgttgtccag acgaacatta agagtaaacg ctgatgccat ttccattatt
     3001 ccgcctccag aaccattgcc gcgccaagcc caccggcagc acaggcggta actaaaccaa
     3061 atccaccgcc gcgacggcga agttcatgca atgtctgggt aatcatccgc gcgccggtcg
     3121 ccgcgaaggg atgtccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca
     3181 cttcgccagt ggcatgtgca cgccccagta cttcacgagc aaaacgttca ctacccagca
     3241 actgaatatt cgccagtgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagtt
     3301 cgctcatcgt caaaccggca cgttccagcg ccagcggtgt tgaccaggct ggaccgagca
     3361 acatgtcctg ccagacatca atcgcagtaa atgcgtagct gcgcagatac cccagcggca
     3421 ccagccctaa ttctttcgcc cgggattcag tcatcaggat caccgctgcc gcgccatcgg
     3481 tcagcggcgt actgtttgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca
     3541 gctttgcgta atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt
     3601 gtttataagg agggataaag gcagtcatca cctcttcttt gagttttccg tctgaccatg
     3661 cctgagcggc acgctgatgc gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc
     3721 cgtaggtttt cgccatttgt tcggcggtgt cgcccatccg caagccagta gaatattctg
     3781 ctaccgcagg cggtacgggc attaagtcgc gcaaacgcag gcgagagaag agtttcaggc
     3841 gctggctcat ggttcgagct ttgttgacat caaccagcac gtacgccagt tttttactga
     3901 cgccaattgg caatacggaa gaggaatctg ccccaccggc aatccccgct cggatagttc
     3961 ccgccatcag gctttctgcg acgtttgcaa ccgcttggaa actggtagcg caagcgcggc
     4021 tgacgctgta agcatcggta tgcacattca ttccggtacc gagaacaatt tcacgcgcaa
     4081 tgttgggggc ttcaggcatt tgtacgacct gaccaaagac cagctgttca atcacttcag
     4141 cggggatctc gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgtgg
     4201 gaatgccatg aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg
     4261 cgatacgatc gccctggcgg gtaaccagtg gtaaaacctg acccataaca ctcccctgta
     4321 aaaaaataaa taaagtggtc tgacctgatc atagtcttaa ccattttttt acatttagcc
     4381 aagcggagaa aaaggaaagt gggagctatg acacagtgaa gacagaagaa agaaaagaaa
     4441 acgcccctgc cagcaactga caggggcgta cgtacccgag aggaattaac gcagacccag
     4501 ctggaagatc agcatttcag cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc
     4561 gtcagactct tcagtgaaag tcggggagat tttgcacggt tcggatttca cgctacggga
     4621 tttttcagtc agcgccgcca gcgtttgttc tgcttctgcg cggtttgcaa acacgcggct
     4681 gtaagacgcg gtgcagtcgg agttgtccat aatggtgcca acatccatac agcagcaaac
     4741 cggggtttca tcagcactac atttactcat cgttgatttc ctctgtatgt gcacccaagg
     4801 tgccagataa acgttgtgga tattttacgc ttccggaaag tgctgctcca gttgttaatt
     4861 ctgcaaaatc ggataagtga ccgaaatcac acttaaaaat gatctaaaac aaaattcacc
     4921 cgaatccgcg agtgcgcaac ctccaaattt tgccagctgg atcgcgtttc ttagatcata
     4981 tttgaaaaaa gatggaaaca tacttgcaac attctagctg gtccgaccta tactctcgcc
     5041 actggtctga tttctaagat gtacctcaga ccctacactt cgcgctcctg ttacagcacg
     5101 taacatagtt tgtataaaaa taaatcattg aggttatggt catgagccag aaaaccctgt
     5161 ttacaaagtc tgctctcgca gtcgcagtgg cacttatctc cacccaggcc tggtcggcag
     5221 gctttcagtt aaacgaattt tcttcctctg gcctgggccg ggcttattca ggggaaggcg
     5281 caattgccga tgatgcaggt aacgttagcc gtaaccccgc attgattacc atgtttgacc
     5341 gcccgacatt ttctgcgggt gcggtttata ttgacccgga tgtgaatatc agcggaacgt
     5401 ctccgtctgg tcgtagcctg aaagccgata acatcgcgcc tacggcatgg gttccgaaca
     5461 tgcactttgt tgcaccgatt aacgaccaat ttggttgggg cgcttctatt acctctaact
     5521 atggtctggc aacagagttt aatgatactt atgcaggcgg ctctgtcggg ggtacaactg
     5581 accttgaaac catgaacctg aacttaagcg gtgcgtatcg cttaaataat gcatggagct
     5641 ttggtcttgg tttcaacgcc atctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc
     5701 tggggcagtt ggttgctggt cagattatgc aatctcctgc cgggcaaact cctcaagggc
     5761 aagcattggc agctaccgcc aacggtatcg acagtaatac caaaatcgct catctgaacg
     5821 gtaaccagtg gggctttggc tggaacgccg gaatcctgta tgaactggat aaaaataacc
     5881 gctatgcact gacctaccgt tctgaagtga aaattgactt caaaggtaac tacagcagcg
     5941 atcttaatcg tgcgtttaat aactacggtt tgccaattcc taccgcgaca ggtggcgcaa
     6001 cgcaatcggg ttatctgacg ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc
     6061 gtgttgatcc acagtgggcg attcactata gcctggctta caccagctgg agtcagttcc
     6121 agcagctgaa agcgacctca accagtggcg acacgctgtt ccagaaacat gaaggcttta
     6181 aagatgctta ccgcatcgcg ttgggtacca cttattacta cgatgataac tggaccttcc
     6241 gtaccggtat cgcctttgat gacagcccag ttccggctca gaatcgttct atctccattc
     6301 cggaccagga ccgtttctgg ctgagtgcag gtacgactta cgcatttaat aaagatgctt
     6361 cagtcgacgt tggtgtttct tatatgcacg gtcagagcgt gaaaattaac gaaggcccat
     6421 accagttcga gtctgaaggt aaagcctggc tgttcggtac taactttaac tacgcgttct
     6481 gataacgcgt tcgcctggat aaagtcacct gctatgcagg tgactttaac tcccccactt
     6541 caccgaaagt agtttcctca ttgcttgcct cctgagtttt gcaaacagcc tgatggcagc
     6601 ttgccctttc aaatcagtaa gtggtatcca cagacaaacc tgaagggaaa tgcatatttt
     6661 caagcgttct gctcgtcctt ctcaaagagt ttacttttct gcatttccag gatactcaac
     6721 ccccctggct attgtgcgct tatacactca aattaaagat aggttctaaa taaatgagcg
     6781 ttttttgata gtctatttca ttaggtaata tatatttgta acaaatcaat caacatggaa
     6841 taaaatcatg ctaccatcta tttcaatcaa caataccagc gcagcttacc cagaatccat
     6901 caatgaaaat aacaatgatg aaattaatgg attagtacaa gagttcaaaa acctttttaa
     6961 tggtaagaaa ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat
     7021 acgagtaaac gacgatcctt atagatctaa tattaataat tcctcagtta cttatattga
     7081 tattggctcc aatgatacag accatattac tattggtatc gacaaccaag aaccaataga
     7141 attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt
     7201 tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata
     7261 tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac atacggtaac
     7321 ggaatttgat gagagcggga aaagcgaacc aactgactta tttacctggt acggtaaaga
     7381 taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc
     7441 tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga
     7501 tagtctcacc caatattgta gtgaaaacgc caggagtgct tcagcgtggt ttgaaagcag
     7561 taaagctatc atggcagaat catttgcaac tggttccgat catcaggttg taaacgagct
     7621 caacggggaa agactgagag aacctaacga agtttttaaa cgtttaggtc gagcaataag
     7681 atataatttt caagtggacg atgcaaaatt tagacgcgat aatgtaaaag aaataatttc
     7741 tactttattc gctaacaaag ttgacgttga ccatcctgaa aataaataca aagactttaa
     7801 gaatct
//
LOCUS       DAZPDE010000169         7585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPDE010000169 DAZPDE010000000
VERSION     DAZPDE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.172"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(349..1749)
                     /gene="asnS"
                     /locus_tag="ACHU0J_003365"
     CDS             complement(349..1749)
                     /gene="asnS"
                     /locus_tag="ACHU0J_003365"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1217916.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1918..3120)
                     /gene="pncB"
                     /locus_tag="ACHU0J_003366"
     CDS             complement(1918..3120)
                     /gene="pncB"
                     /locus_tag="ACHU0J_003366"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1217917.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3386..6001
                     /gene="pepN"
                     /locus_tag="ACHU0J_003367"
     CDS             3386..6001
                     /gene="pepN"
                     /locus_tag="ACHU0J_003367"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1217918.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6208..6975)
                     /gene="ssuB"
                     /locus_tag="ACHU0J_003368"
     CDS             complement(6208..6975)
                     /gene="ssuB"
                     /locus_tag="ACHU0J_003368"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1217919.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6972..>7585)
                     /gene="ssuC"
                     /locus_tag="ACHU0J_003369"
     CDS             complement(6972..>7585)
                     /gene="ssuC"
                     /locus_tag="ACHU0J_003369"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1217920.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1933 a         2004 c         1912 g         1736 t
ORIGIN      
        1 tgatgatatt tacagcaatt tttatctatt tatatgattt acttatattt aaattaacta
       61 aacggaaatt ttgtttctga tggaaacttt atcgacctgg cacaaaatcc tctttcagac
      121 atccagaatg ccaaaaaata gtatgaaaat tgtgctatca gctaagaaaa aactaatccg
      181 caataagagt acgggttatt ttttgtgcgt aaatgttgca atgtaatatt ccgtaacagg
      241 atgatcgtta tggcgggggt gcaaatagat tgcttgactt aacaaacaga taacttgaca
      301 gaaaggataa aaaagagcca gcaggcgctg gctctttgag gaagaaaatc agaagctggc
      361 gttgcgcgga gtacgtggga acggaatcac atcacgcacg ttttgcacgc cagttacgta
      421 agcaatcagg cgttcaaaac caagaccgaa acctgaatgc ggaacagtac cgtagcgacg
      481 cagatcgcga taccaccagt aatcttcttt attcaggccc atttccagca tacgctcgtc
      541 cagaacgtcc agacgttctt cacgctggga gccaccaatg atctcaccga tgcccggagc
      601 cagaacgtcc atagccgcaa cggttttacc gtcttcgttg aggcgcatat agaacgcttt
      661 aatatctttc ggatagtttt taaccactac cggtgcttta aagtgttctt ccgccagata
      721 acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc gggttttcaa acttcctgcc
      781 gcagttttcg agaatggtca ctgcgtcggt gtaatccacc tgcgcgaaat cggcttcaat
      841 gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt tcagcgaaga atttcatgtc
      901 gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc agcatggctt cagccagacc
      961 cgcgatatcg ttcaggttag caaacgccac ttccggctcc agcatccaga attccgccag
     1021 gtgacggctg gtgttggagt tttcagcacg gaaagtcggg ccgaaggtat aaattttgga
     1081 taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat acggtcagga aagactcttt
     1141 accaaagaag tctttgtcga aatccacttt gccctgatcg ttacgcggca ggttttccag
     1201 atccagcgta gaaacgcgga acatttcgcc tgcaccttcg gtatcagatg cggtaatcag
     1261 tggcgttgaa acccagaaga atccctgctc gttaaagaag cgatgcagcg cctgcgccag
     1321 cgtatggcga acgcgcgcga cggcaccaat caggtttgtg cgcggacgca ggtgagcgac
     1381 ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc gggtaagtgt ctggatcttc
     1441 aacccaacca gcaacttcaa ccttgctggc ctgaatttca aattgttgcc cctggcccgg
     1501 cgatgccacg actttacccg tcacaatgac cgagcagccg gtggtcagac gcaggacgtc
     1561 ttcattgtaa ttgggcagag aattattgat gacagcctgt acaggatcaa agcaggaacc
     1621 gtcataaacg gcgaggaagg agatgccagc ttttgaatct cggcgggtac gtacccatcc
     1681 gcgtacggtg acttcgctgt caacggctac gcggccctgg agtacgtcgg ctacaggcac
     1741 aacgctcata atattctctc tgttaatagt cggaaaaaat aaacacttgt ccacccgtaa
     1801 atggggggga tacctatgtt acctggcatc tgcaatcaga caagcagaat tcgcaaatgc
     1861 agcgaaagat ttcggaatta aaggtaaaaa agggagccga ttagctccct gatgatatta
     1921 actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc agcgcccgaa caaacgcttt
     1981 atcatggcag atagttttgc cagggctgtc agaaagtttc gccaccggtt taccgttaca
     2041 ctctaccaac ttaatcacaa tattcagggg ttttacctgg gggatatcgc aggtcaggcg
     2101 agtcccaata ccaaaactta attgcacgcg ggaagagaag tggcggtata gctcaaccgc
     2161 tttgcgtaaa tccagattgt cagagaaaac cagcgtttta ctctgtggat caattcccag
     2221 cttttcataa tgtgcaatgg ctttttcacc ccattcaacc gggtcgccag agtcatgacg
     2281 caggccctga taccgactag cgaactcgac accgaaatca cgcaggaaag catccatagt
     2341 gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac tcttccagcc aggcagcaag
     2401 tgcagctcgc tggctgttgg ctagatccgg gctgatttgc tgatgtgcct ggaaccattc
     2461 gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc gccagatcgt agttgctggt
     2521 gcccacgaac caggattcct gttgcagacg cttaacgatg gtttcttgta cttcgcgaga
     2581 aaaacggcga cgggtgccaa aatccatcag atggaagcgc gacatatcaa gaccggcggt
     2641 taacgccgag aagtcgacta atttgctttc cagcgtgtcg agggcttgcg caacgtcggc
     2701 ctgcggtgag cgatagcgat gtaccatttc actgataacc gccagcaaag gaacttccca
     2761 gaggatgact tcacgccacg gaccgcttaa gcgaatatcc agcttgccat tatcgttgga
     2821 cacggtgact tgttccgggt taaagcggaa ctcgcgtaac cagttaagat agtcggcctt
     2881 aaagaaaggc agggcagaga gccactgata ttcatcatcc tgcaggcgca ggtgctgcat
     2941 cgcctgaatc tgttcacgaa tagcatcggc ataaataccc agcagatcgt cacctcggca
     3001 acgaaactcc gccgcgacat gcacatcgta atagtgatga aacacggctt gctgcatatg
     3061 caacttataa gcatctgtat ccagcaacga gtgcagaaca ggagaagcga attgtgtcat
     3121 aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca ataaacatct tcaggaactg
     3181 ctggagtata ccttgtttcg caatttattg aaccccgatc acaccatatg ccacctttct
     3241 ggtcgatggc attacgcggt gtatgttata aaaatgtagc aataaaggcg tttgtacctg
     3301 aaaagatgaa gattctgcat agcgcgattt acgcaacagg aatagactga acaccagact
     3361 ctataaaaga tgctaaaggt tatttatgac tcaacagcca caagccaaat accgtcacga
     3421 ttatcgtgcg ccggattacc agattactga tattgacttg acctttgacc tcgacgcgca
     3481 aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat ggtgcatcag atgctccgct
     3541 tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat attaatgatg agccgtggac
     3601 cgcctggaaa gaagaagaag agggcgcact ggtcatcagt aatttgccgg agcgttttac
     3661 gcttaagatc attaatgaaa taagcctggc ggcgaatacg gcgctggaag ggctttatca
     3721 gtcaggcgat gcgctttgca cccagtgtga agccgaaggt ttccgccata ttacgtatta
     3781 tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa attattgccg ataaaatcaa
     3841 atatcccttc ctgctttcca atggtaaccg cgttgcgcaa ggcgaactcg aaaacggacg
     3901 ccattgggta cagtggcagg acccgttccc gaaaccgtgc tacctgtttg cgctggtggc
     3961 aggcgacttt gatgtactgc gcgatacctt taccacgcgt tctggtcgcg aagtagcact
     4021 ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg tgggcgatga cctcgctgaa
     4081 aaactccatg aaatgggatg aagaacgctt tggcctggag tatgacctcg acatctatat
     4141 gatcgtcgcg gtggatttct tcaatatggg cgcaatggag aataagggtc tgaatatctt
     4201 taactccaaa tatgtgctgg cccgcaccga caccgccacc gacaaagatt acctcgatat
     4261 tgaacgcgtt atcggccatg aatatttcca taactggacc ggtaaccgag tgacctgccg
     4321 cgactggttc cagctcagcc tgaaagaagg tttaaccgtc ttccgcgatc aggaattcag
     4381 ctctgacctt ggttcccgcg cagttaaccg catcaataat gtgcgcacca tgcgcggatt
     4441 gcagtttgcg gaagacgcca gcccgatggc gcacccaatc cgcccggaca tggtcattga
     4501 gatgaacaac ttctacaccc tgaccgttta cgagaagggc gcggaagtga tccgtatgat
     4561 ccacaccctg ctgggcgaag aaaacttcca gaaagggatg caactctatt tcgagcgtca
     4621 tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg atggaagatg cgtcgaatgt
     4681 tgatctctcc catttccgcc gttggtacag ccagtccggt acgccggttg tgaccgtcaa
     4741 agacgactac aatccggaaa ctgagcagta caccctgacc atcagccagc gcacgccagc
     4801 cacgccggat caggcagaaa aacagccgct gcatattccg tttgccatcg aattgtatga
     4861 caacgaaggt aaagtgatcc cgttgcagaa aggcggtcat ccggtgaatt ccgtgctgaa
     4921 cgtcacccag gcggaacaga cctttgtttt tgataatgtc tacttccagc cggtgcctgc
     4981 gctgctgtgc gaattctctg cgccagtgaa actggaatat aagtggagcg atcagcaact
     5041 gaccttcctg atgcgtcatg cgcgtaatgg tttctcccgt tgggatgcgg cgcaaagttt
     5101 gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag caagggcagc cgctgtctct
     5161 gccggtacat gtggctgacg ctttccgcgc ggtgctgctc gatgagaaga ttgatccggc
     5221 gctggcggca gaaatcctga cgctgccttc tgtcaatgaa atggctgaac tgttcgatat
     5281 catagacccg attgctattg ccgaagtacg cgaagcactc actcgtactc tggcgactga
     5341 actggcggat gagctgctgg ctatttacaa cgcgaattac cagagcgagt accgtgttga
     5401 gcatgaagat attgcaaaac gcactctgcg taatgcctgc ctgcgcttcc ttgcttttgg
     5461 tgaaacgcat ctggctgacg tgctggtgag caagcaatac catgaagcaa acaatatgac
     5521 cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag ctgccttgcc gtgacgcgct
     5581 gatgcaggag tacgacgata agtggcatca ggacggtctg gtgatggata aatggtttat
     5641 cctgcaagcc accagcccgg cggcgaatgt gctggagacg gtgcgtggtc tgttgcagca
     5701 tcgctcattt accatgagca acccgaaccg tattcgttcg ttgattggtg cgtttgcggg
     5761 cagcaacccg gcagcgttcc atgccgaaga tggcagcggt taccagttcc tggtggaaat
     5821 gcttaccgac ctcaacagcc gtaacccgca ggtagcttca cgtctgattg aaccgctgat
     5881 tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg cgcgcggcgc tggaacagtt
     5941 gaaagggctg gaaaatctct ctggcgatct ttacgagaag ataactaaag cactggcttg
     6001 atacttttga gccaaaatgc ctgatgcgac gcttgacgcg tcttatcagg cctacaactc
     6061 cgttgaaatt gctggttttg taggctggat aaggcgttca cgccgcatcc ggcagccgtg
     6121 cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc ctacaagtcc gttgaaaatg
     6181 ctggttttgt gcgccacatc cggcatatta accatgacgc cgaatcagtt gttcagaatg
     6241 cccacgccgc attacccgtt gcaatacttc cgcttccagc tccgctaatc gcaccgaccc
     6301 taatcgacgc ggacggggga tatccactgt caaatccaga ctaatttttc cctcttcaat
     6361 taacaacacc cggtcagcca ttgctaccgc ttcgctcaca tcatgcgtca ccaacagtac
     6421 ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc tgcatctcga gtcgcgttaa
     6481 ggcgtccagt gccccgagcg gttcatcaag caacaataaa cccggtcgat gaatcaacgc
     6541 tcttgccagc gccacacgct gtttctgccc gccagaaagt gcggcaggcc attccccggc
     6601 gcgattctcc agtcctgccg cagccaacgc ccgacgcgcg gcatcgcgcc actgaccttt
     6661 aaggcctaac ccaacgttat caatcaccga tttccacggc agcagacgcg catcctgaaa
     6721 catcattcgc gtatcatcct gaatttcagc cagcggtgtg gtgcccgcta acacatcgcc
     6781 tgcggttggc gtttccagac cggccagcag gtgcagcagg gtacttttac caccaccgct
     6841 gcggcccacc accgccacaa actgacctgc cggaatatgt aaatccagtt ggttcaggac
     6901 gatattttcc gcgtaatgtt tgcttactgc attgagcaac aatggtgtgc cctggttcag
     6961 acgagcagta ttcatatcgt ggcctccttc aaatgataag ccgggttcca gcgcaaccag
     7021 aggcgctcta acagctgcgc gctgacgtca gcaagtttgc cgagcagggc gtaaagaata
     7081 atagcgacta ccaccacgtc tgtttgcaaa aactctcgcg cattcatcgc cagataacca
     7141 atgccggaat tggcagaaat ggtttcggca acaatcagcg tcagccacat caggcccaac
     7201 gcaaaacgca cgccgaccat aattgagggc agggcatcgg gcaggatcac atggataaac
     7261 agcggtatgc cggataatcc atagctacgc gccatctcca ccagcccgcg atcgatatta
     7321 cggatgccat gccaggtgtt gatataaatg gggaacagcg taccgagcgc caccagaaag
     7381 attttcgcgg attcatcaat gccaaaccac aaaatcacca gcgggatcag cgccagatgc
     7441 ggcacgttgc gcaacatctg aattgaggta tccagcagcc tttctcccca gcgcgacagt
     7501 ccgctaatca gtcccagaat caggcccagc gatccgccaa ttgaaaagcc aatcagcgcc
     7561 cgccaggaac tgatcgccag atgct
//
LOCUS       DAZPDE010000170         7537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPDE010000170 DAZPDE010000000
VERSION     DAZPDE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.173"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHU0J_003370"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHU0J_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHU0J_003371"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHU0J_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1217921.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHU0J_003372"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHU0J_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHU0J_003373"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHU0J_003373"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1217922.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHU0J_003374"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHU0J_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1217923.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHU0J_003375"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHU0J_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1217924.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHU0J_003376"
     CDS             complement(6512..7204)
                     /locus_tag="ACHU0J_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1217925.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1826 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac cccccgcgcc gctgagaaaa agcgaag
//
LOCUS       DAZPDE010000171         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPDE010000171 DAZPDE010000000
VERSION     DAZPDE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.174"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHU0J_003377"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHU0J_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR1217926.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHU0J_003378"
     CDS             complement(1433..1759)
                     /locus_tag="ACHU0J_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR1217927.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHU0J_003379"
     CDS             1894..2235
                     /locus_tag="ACHU0J_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR1217928.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHU0J_003380"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHU0J_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHU0J_003381"
     CDS             complement(2833..3543)
                     /locus_tag="ACHU0J_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR1217929.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHU0J_003382"
     CDS             3651..3956
                     /locus_tag="ACHU0J_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR1217930.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHU0J_003383"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHU0J_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHU0J_003384"
     CDS             complement(6298..7287)
                     /locus_tag="ACHU0J_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217931.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZPDE010000172         7299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPDE010000172 DAZPDE010000000
VERSION     DAZPDE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.175"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHU0J_003385"
     CDS             complement(<1..190)
                     /locus_tag="ACHU0J_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1217932.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHU0J_003386"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHU0J_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1217933.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHU0J_003387"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHU0J_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1217934.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHU0J_003388"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHU0J_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1217935.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHU0J_003389"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHU0J_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHU0J_003390"
     CDS             complement(3262..3381)
                     /locus_tag="ACHU0J_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1217936.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHU0J_003391"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHU0J_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1217937.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHU0J_003392"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHU0J_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1217938.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHU0J_003393"
     CDS             3803..4147
                     /locus_tag="ACHU0J_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1217939.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHU0J_003394"
     CDS             4151..4486
                     /locus_tag="ACHU0J_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1217940.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHU0J_003395"
     CDS             complement(4543..6864)
                     /locus_tag="ACHU0J_003395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1217941.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHU0J_003396"
     CDS             complement(6981..7190)
                     /locus_tag="ACHU0J_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217942.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         1996 a         1730 c         1622 g         1951 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaattta
//
LOCUS       DAZPDE010000173         7298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPDE010000173 DAZPDE010000000
VERSION     DAZPDE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.176"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(2..94)
                     /locus_tag="ACHU0J_003397"
     tRNA            complement(2..94)
                     /locus_tag="ACHU0J_003397"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHU0J_003398"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHU0J_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1217943.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHU0J_003399"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHU0J_003399"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1217944.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHU0J_003400"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHU0J_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1217945.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHU0J_003401"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHU0J_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1217946.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHU0J_003402"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHU0J_003402"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1217947.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHU0J_003403"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHU0J_003403"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1217948.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1697 a         2052 c         1765 g         1784 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacata
//
LOCUS       DAZPDE010000174         7288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPDE010000174 DAZPDE010000000
VERSION     DAZPDE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.177"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(96..299)
                     /locus_tag="ACHU0J_003404"
     CDS             complement(96..299)
                     /locus_tag="ACHU0J_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217949.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            377..1471
                     /gene="ddlA"
                     /locus_tag="ACHU0J_003405"
     CDS             377..1471
                     /gene="ddlA"
                     /locus_tag="ACHU0J_003405"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1217950.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1495..1707)
                     /locus_tag="ACHU0J_003406"
     CDS             complement(1495..1707)
                     /locus_tag="ACHU0J_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1217951.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1967..2275
                     /locus_tag="ACHU0J_003407"
     CDS             1967..2275
                     /locus_tag="ACHU0J_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1217952.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2334..3428)
                     /gene="yaiW"
                     /locus_tag="ACHU0J_003408"
                     /pseudo
     CDS             complement(2334..3428)
                     /gene="yaiW"
                     /locus_tag="ACHU0J_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3441..4661)
                     /gene="sbmA"
                     /locus_tag="ACHU0J_003409"
     CDS             complement(3441..4661)
                     /gene="sbmA"
                     /locus_tag="ACHU0J_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1217953.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            5013..6170
                     /gene="ampH"
                     /locus_tag="ACHU0J_003410"
     CDS             5013..6170
                     /gene="ampH"
                     /locus_tag="ACHU0J_003410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1217954.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6171..6674)
                     /locus_tag="ACHU0J_003411"
     CDS             complement(6171..6674)
                     /locus_tag="ACHU0J_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1217955.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6762..>7288)
                     /locus_tag="ACHU0J_003412"
     CDS             complement(6762..>7288)
                     /locus_tag="ACHU0J_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1217956.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1864 a         1848 c         1741 g         1835 t
ORIGIN      
        1 aggaaatact ttgcgcaaat attttttgtt tgattgccag cgaaacgttt ctcttattta
       61 aataattacc cacacaaaat ataggctttt aattatcaac ctttattata aataaaagct
      121 atgaaatata ttctcttcgt taacttataa ttaagagaaa aaaactcacc gcattgccat
      181 ttaagccagg aataaattag cttcccgttc atcatttttg tgatcttgcg cacatctttg
      241 agataacgca ctttcttcac cagtacgttg cacgctttgt cagcagaaga ttgcaacaat
      301 ctttcagaag gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt tgtgttagac
      361 gggataggtt tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg gtaaatcagc
      421 ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc gatgccattg ataaaagtcg
      481 cttcgacgtt gtgctgctgg gcattgataa acaagggcaa tggcacgtca gcgatgccag
      541 caattatctg ctaaatgcag acgatcctgc ccatattgcg ttgcgccctt cggcgaccag
      601 ccttgcgcag gtgccaggta aacatgagca tcagcttatc gacgcgcaaa acggtcagcc
      661 gttgccgacg gtggatgtca ttttcccgat tgtccacggt acgctgggcg aagatggttc
      721 cttgcaggga atgctgcggg tcgccaattt accgtttgta ggttctgatg ttctggcttc
      781 agcagcctgt atggataaag atgtcaccaa acgtctgcta cgcgatgccg ggctgaacat
      841 tgcgccattt attaccctga cgcgcgctaa tcgtcacaac atcagttttg ccgaagtgga
      901 gtctaaactg gggttaccac tgtttgtaaa accggctaat cagggctctt ctgttggtgt
      961 cagcaaagta accagtgaag aacagtacgc aattgccgtc gatctggcgt tcgagttcga
     1021 tcataaagtg atcgttgagc aagggatcaa aggtcgtgag atcgaatgcg cagttctggg
     1081 caacgacaat ccgcaagcca gcacctgtgg cgagatcgta ctcaccagcg atttctatgc
     1141 ctacgacacc aagtacattg acgaagatgg cgcgaaagtg gtagttccgg cagccattgc
     1201 accagagatc aacgataaga tccgggcgat tgccgttcag gcttatcaga cgttgggatg
     1261 cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg tgatcaacga
     1321 gatcaacaca ctgcctggct tcaccaacat cagtatgtat ccgaagctgt ggcaagccag
     1381 cggtctgggt tacaccgatc tgatcacacg tttgattgag ctggcgctgg agcgtcacgc
     1441 tgcggataac gcattgaaaa ccacaatgta atattttaac gcccgataat attcttattg
     1501 ggcgttttca gtttcttcat cgcgacgacg aataatcaac cccgccagcc agaaactaat
     1561 cacccacgtc accagaccga cggcataggt ctgccaacct tttgcttcaa atcccagtac
     1621 ccccaccacg ccattaagaa tgaatataag gccaatggcg aacgcatagt agtgccagtc
     1681 acggcggatt tttacaggca gattcatggc agctccgtta acgaaaaaca acatccttgc
     1741 atattctgta acaaccgtct gtggtgtaga caacattttc gtaattatta ataaatttaa
     1801 cttaaggttg ccattttgtg actcacaacg aagattcacg cttcgggaat attcccaaca
     1861 tcaatttacc atgattctga tattgacaat ctaaatgacc tgtcagactt gccataagcc
     1921 gctggtatca caccagaaga gaagcagatg acttccggag gtttttatgg ctgatttcac
     1981 cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc ctggcaacat
     2041 tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc tgaacctgtt
     2101 agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc caggaaacag gtcgcccacg
     2161 ggtggaaatt ggtttaggtg tcggcaccat tttcgggctg atcccgtttt tagcaggctg
     2221 cctcattttt gcagtggtgg cgctatggct gcactggcga catcgccgcc agtaaaattt
     2281 acgttgccag atgcggcctg aaacgcctta tctggcctga cattcctgtt tgatcactgt
     2341 ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg
     2401 cgggtgattt tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg tagtgattta
     2461 cccgttttcg cttcggcaag ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag
     2521 aagctgtcac ctttctctaa ctgacggcga atttcgctgt cgctcatccc cagttttccc
     2581 gccagtttgc gcgttgccag ttcggttttc ccgggttctt tactgtcgta acgaattaaa
     2641 tcaccgtcca gtgccagctt cacgccgctg gccttactga ctgcgttctg aaacgcggca
     2701 ttacggctgg cgtaccagcc agcgttaaaa tcagcaaagc ggtatatcgg tgcgctatag
     2761 ctggcgggat aattcagtaa atggtaagta ccaaaccaca acccgccgcg gcgactgaag
     2821 acttcctgac gaactgtacc gtccattttc cacggatacc ctttggtatg ctgttcggca
     2881 aaagcaatgc tgacctgcat cggcccaccg gtgcgcaccg gattaagcga accaaacagc
     2941 gtctgcccca ttggtaccat gctgatcaga tcgtcaaaaa tcgcgctcaa ttgcttttct
     3001 gtacgcaccg aatctaaccg ttcgctataa cttttgccgt ttggcgattt gatttttagc
     3061 gcggtatgaa ccagaaaagc aggaatgtgc atccgttcag cacgacggtc aatctcttgc
     3121 taggcgatct tgcttaagcc cggaacggcc ggatctgcct gataatttga ctcctgctgc
     3181 gccaccgcca gcaccgagta gacgttttcc agcgtcggtg ccagcccctg actttcaaag
     3241 gtggtggcga gatctttcgc ccaggcatcg cggtctttca cgctcgcagg catcttctgc
     3301 cgcacgacgc tcgctacatc tatcgctttt tcgccctttt tcaacggttg cggagcctga
     3361 ctactacacc ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag atgattgacg
     3421 cgcgacatta cttctccttt ttagctcaag gtatgggtta cttcctgaat tttgtcacca
     3481 tccagctcat gttcaaagct gcgcagacgt ttgtagatag acatcaactc aaccagtgtg
     3541 gtccatgagt taatcaggta ctggaaagca ccgcgaacct gaccaaaaac gttggtaatc
     3601 tgcgtcatca ggccgagcgt aatcgtaccg gcaacaattg acggaaacag caagaacaaa
     3661 ccgaaaacgt tatcgacctg caaatagaga atgcgggcga tgttgaaata catatagtga
     3721 aaatagaggc gaaaatagtt tttccgtacg gcgctaaaca gctcgcgtac cgtaggcggc
     3781 gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt tacggtaggc ggcctctact
     3841 cgctggtttt taaactccag ccccggcagt ttgatcccta ccactgccag caatccggtc
     3901 cccatcagcg accagacgat agcggcaatc accagaccat acggaatgtg cccgataatc
     3961 ggcagctccg gcacatgcgc ggagagcgtt accagcaccg gcaggaaggc gatcaacgtc
     4021 atgatggcgt tgataaaact gacgcccata ttctccagcg ttgaagcaaa acgcatggtg
     4081 tcttcctgca cacgctgtgc ggcaccttcg atatgacgca gttgttgcca gttcgccatg
     4141 taatattcgt tcatcgccgt acgccagcgg aacacgtagt gactgacaaa gaagttgttc
     4201 agtacactga tcaccaccgc gatcagcgca atccccagaa agacgcccac ttcgcggtaa
     4261 aattgttcga tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag atcatagaac
     4321 ggcgcatacc aggcgttgac ggcgaccccg acttccacca aaaaccaagt gacgaagata
     4381 atcagcgcag taccgagtat tgaccagtat tgccaacggt gcgggctgta gataaaccag
     4441 aaaaatgcaa aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag gaaatccaac
     4501 gaccagaaac gcgcggcgct aatcgggatc tgcccggaag cgccggtgat acgtgccacc
     4561 cagtcacccc caccggcttg ccagaagata acggcaatca atgcccaaac aaaggccgag
     4621 agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc
     4681 ttatcgtttt aacaactgct gtgtattaac cgcatgaccg ctacaaagcg tcagccaaat
     4741 gaaaccgccc attgtgaccc atagcaaggc gcttagttcg ccagcctgac cataagaatt
     4801 gtttcgacgg atttcacaaa ccaaagattg tttacataag cggattaaca cgcgcaggat
     4861 cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta
     4921 acactattat tgcactaatt cgccctttgc aatccatcaa tgagtagtat aaatacgctc
     4981 agttaccttc attcaatcta tggacaccac cgttgaaacg tagtctgctt ttttctgccg
     5041 tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca gccgatcact gaaccggagt
     5101 ttgcctctga tattgtcgat cgttatgccg atcatatttt ttacggcagc ggtgccacgg
     5161 ggatggcgct ggttgttatc gacggtaacc agcgcgtctt tcgtagttat ggtgaaacgc
     5221 gacctggtaa taacgttcgc ccgcagctgg attcggtcgt gcgtattgct tccctcacca
     5281 aactaatgac cagtgaaatg ctggtgaaat tgctcgacca ggggaccgta aagttaaacg
     5341 atccgctaag taaatatgct ccgccaggcg cacgcgtgcc gacctacaat gggacgccca
     5401 ttaccctagt gaatctggca acccatacca gcgccctgcc ccgtgaacag cccggtggcg
     5461 cggcacatcg tccggtgttt gtctggccaa cgcgcgagca acgctggaaa tacctttcta
     5521 cggcgaagct gaaagccgcg ccaggcagtc aggcggcgta ctctaacctt gcgttcgacc
     5581 tgctggcaga tgcactggcg aatgcgtccg gcaagcctta tacccagttg tttgaagagc
     5641 agattaccca tccattgggg atgaaagaca ccacctacac cccctcaccg gatcagtgcc
     5701 gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg
     5761 gcagcggcgg cgtctattcc acgcctggcg atatgatgcg ctggatgcag cagtatttgt
     5821 catctgattt ttatcagcgc agtaaccagg cagatcgcat gcagacgttg atttatcagc
     5881 gcgcgcagtt tacgaaagtg attggcatgg atgtccccgg caaagcggat gcgcttggtt
     5941 taggctgggt atatatggcc ccgaaagagg gtcgtccgag gattattcag aagacaggcg
     6001 gtggtggcgg tttcattacc tatatggcga tgatcccgca gaagaatatt ggcgcgtttg
     6061 tggtggtcac tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc atcaatgatc
     6121 tggtgaccga gctaagcggg aataaaccgt tggttatccc cgcatcctga ttaatactct
     6181 gaaggcaaac ggttgatagc gaccagtttg cctttattca tttcgatata gccgccttta
     6241 cgcaaagcag cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg ttgatggata
     6301 taattcatca caccaatacg tgatcgcaat tcttcattcc agtcaatcat tgataataat
     6361 gttgcgcggg tttgtttggc tggtagatga tatcccgtac aatttccttc tgatattaat
     6421 ttgtatggta tatcgttttt cattaaacca tcagccagcc ccataatata aggagcctgg
     6481 gtaataccga taagtacgtt ttcttctcta cgcagagaga taactccttc taaaataacg
     6541 aaagtatctt cgttattgac atcactggaa aatataactt gcttttcatt attaaactcg
     6601 aagcgcgtac cgtatctgga caaacattta tcgagcttac caaattcctg aagaggttta
     6661 actacagata acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt
     6721 atttagatta aattaccagt atttcttcgg agtgaagaat attaccaagt atatttaaca
     6781 cccacgttcg cggaccagtc ttgatctacg tcaccaccac cgaggtagtt agcatcggta
     6841 taggcgctga agttcttggt gaagctgaac tgagtaccca gaccaacacg taccgcagac
     6901 ccttcagtac cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg
     6961 tagacgtaag ccagtttgaa gtacggagtc agagcctgat cttcgctgta ggtgaaggta
     7021 taacctgcat ctacacccag ttcataacgc atgctgtcgt aaggctgacc gtcaactttc
     7081 atgtcgttgc tcagctggta gtcatcacca gactggaaca gaccagaaat gctgccgtaa
     7141 ggagtcacgt aaccagcatc acccagtttg aagtcgtaac cggttttcaa accgaagccc
     7201 caagcgtcgg agttggtgct accgtcaacg taagtaccgt tgctcatggt tgcagacagg
     7261 tcgttgttga agtgagagta gctcaagc
//
LOCUS       DAZPDE010000175         7284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPDE010000175 DAZPDE010000000
VERSION     DAZPDE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.178"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHU0J_003413"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHU0J_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR1217957.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="ACHU0J_003414"
     CDS             1104..2564
                     /locus_tag="ACHU0J_003414"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1217958.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHU0J_003415"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACHU0J_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1217959.1"
                     /translation="MATYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHU0J_003416"
                     /gene_synonym="ydfH"
     CDS             complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACHU0J_003416"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1217960.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHU0J_003417"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACHU0J_003417"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1217961.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="ACHU0J_003418"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="ACHU0J_003418"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1217962.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="ACHU0J_003419"
     CDS             complement(6729..7247)
                     /locus_tag="ACHU0J_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1217963.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1795 a         1726 c         1871 g         1892 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgc cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg attt
//
LOCUS       DAZPDE010000176         7201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPDE010000176 DAZPDE010000000
VERSION     DAZPDE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.179"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHU0J_003420"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHU0J_003420"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1217964.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHU0J_003421"
     CDS             complement(1672..2667)
                     /locus_tag="ACHU0J_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1217965.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHU0J_003422"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHU0J_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHU0J_003423"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHU0J_003423"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1217966.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHU0J_003424"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHU0J_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1217967.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..>7201)
                     /gene="emrK"
                     /locus_tag="ACHU0J_003425"
     CDS             complement(6108..>7201)
                     /gene="emrK"
                     /locus_tag="ACHU0J_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1217968.1"
                     /translation="FIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVV
                     NHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASAR
                     IQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNT
                     PLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGYIAQRSVQVGETVSSGQSLMAVVPA
                     RQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
                     ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPDLASTVTSM
                     PAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1985 a         1710 c         1664 g         1842 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 a
//
LOCUS       DAZPDE010000177         7183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPDE010000177 DAZPDE010000000
VERSION     DAZPDE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.180"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHU0J_003426"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHU0J_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHU0J_003427"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHU0J_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR1217969.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHU0J_003428"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHU0J_003428"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR1217970.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHU0J_003429"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHU0J_003429"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR1217971.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHU0J_003430"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHU0J_003430"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR1217972.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHU0J_003431"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHU0J_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHU0J_003432"
     CDS             7090..>7183
                     /locus_tag="ACHU0J_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR1217973.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZPDE010000178         7182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPDE010000178 DAZPDE010000000
VERSION     DAZPDE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.181"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(94..2097)
                     /locus_tag="ACHU0J_003433"
     CDS             complement(94..2097)
                     /locus_tag="ACHU0J_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217974.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2094..3230)
                     /locus_tag="ACHU0J_003434"
     CDS             complement(2094..3230)
                     /locus_tag="ACHU0J_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1217975.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3223..5502)
                     /locus_tag="ACHU0J_003435"
     CDS             complement(3223..5502)
                     /locus_tag="ACHU0J_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR1217976.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5513..6601)
                     /locus_tag="ACHU0J_003436"
     CDS             complement(5513..6601)
                     /locus_tag="ACHU0J_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR1217977.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1679 a         1931 c         1917 g         1655 t
ORIGIN      
        1 ggtgactctc accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga
       61 actggagtag aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa
      121 taattgttcg caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt
      181 attacgaaat tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt
      241 cgccagcaac tctgcctgat taatagcgct ctgttcctga tagcgccagc cgttatggag
      301 cagttgaata agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg
      361 cagaacgctg gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca
      421 ggcacgagtc tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact
      481 gacaaagcca gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg
      541 ttcaatggca taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc
      601 accttcgccg ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga
      661 aatacactca gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca
      721 ggcgggggga cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg
      781 cgcgctcaac atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg
      841 ggtagctaac agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc
      901 acccgccagt gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc
      961 ctgttcactt cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt
     1021 aaaccagatg cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac
     1081 cagccgcaga tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca
     1141 cggcacagga ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa
     1201 tcgactgttt aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg
     1261 ggcgtggaag gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca
     1321 gctgcagcgt tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg
     1381 ctggctgatg ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt
     1441 tttgccttct tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg
     1501 ccagattaaa tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc
     1561 cagtacgaca tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact
     1621 gctgcgggaa taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg
     1681 cgcacagaac aactcaatgg tgatgctttt agcgaccagc gcctgtgcgc gtttgcgggt
     1741 ggcatcggga agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt
     1801 tcccgtaggc tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg
     1861 acggcacatg ccgctggccc cataggtgca ctgagcctct ttcagtgcct ggccgttcgc
     1921 cagctgggta cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat
     1981 ttccgggacg ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct
     2041 taacgccgtc agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac
     2101 tgaagattct ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg
     2161 ccgacattaa ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg
     2221 agcgctgcca gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc
     2281 agcaatgatg atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca
     2341 ggttgttcaa taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct
     2401 aattgcactt tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg
     2461 cttgtgtcaa acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc
     2521 atcacagcag agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc
     2581 agttgccatt gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac
     2641 aacttgccgt gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca
     2701 aagtttcgcg ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc
     2761 tgacgaactt cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg
     2821 cgggcggcag ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc
     2881 aggctttcgg aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg
     2941 ccgtagcgca gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg
     3001 tgaatactgt tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt
     3061 tcaccacgct ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc
     3121 tgccgggcgt tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat
     3181 cgccagcgtt gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc
     3241 ccagtgttgc agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg
     3301 cggtggacaa tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg
     3361 ctcaagcacc tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc
     3421 gcgtaaatcg ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct
     3481 gaaaccggcg ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc
     3541 gtgcagcgcc tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc
     3601 ggcactagcg ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag
     3661 tgccaggaca tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga
     3721 aacgccgggg agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca
     3781 gctatgcaag tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca
     3841 cagcgtgcga tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc
     3901 gctaatttca tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc
     3961 aaattgattt tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga
     4021 cgccagaccc gctgacgccg cttggctaag acatgtggta atgctgccga ttccacccgc
     4081 gtccagcata tcggcttcta atttatggcg tgcggcttcc aggagtgtgg caccaaagca
     4141 ggcggcctca atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc
     4201 accgttgcct gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag
     4261 tattgccagc cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa
     4321 ggtaagctcc gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg
     4381 cagaggcggt tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc
     4441 tatcaacgtt agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg
     4501 caacggcgca ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt
     4561 gcgtaacggt agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc
     4621 agccatatcc gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt
     4681 tagcagttgt tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg
     4741 ggcaggcatt cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggt
     4801 gagataacaa cctgtaacgg catctgccag cgacggcaat tctggcgtgt taattacctg
     4861 cggacattcg cgccacattt ttgccagtgc cggagcgtgc cagccaccgc agacaactaa
     4921 cacgtcgcca ttattttgct gcatcgccca tccaatccag cgggccataa aggcttcgcg
     4981 ctgacgattt agagcatcgc cgggggagtc gccccgcagt tgggcaaaat agtgcgccag
     5041 tgcagagggt aatgcagttt gctggctttc atcttcgaac aagtgatccc acagggtgtc
     5101 gctgttatcc atgcgggtgg cacgcagcag caaggtctgg ctttcctctt gcgtatcagg
     5161 cgaatcgtcc tcttcttcac tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc
     5221 ctgaatacga cgatcggctt gtagcgcctg ccactccggc gaaaattcag caaatggcgt
     5281 ccaggcacca cgcccggggg ctgcaccgtc ctgatactgg caataactgt aaatagctac
     5341 cggaagctgg tgggctaaaa acagttcgtc tacccggtca ttaaaatcag cagggccttc
     5401 aatcaacacg tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc
     5461 cggactatga tgccggatgc cgacaattaa cggctcgctc atgcatcttt cctcacggca
     5521 ggcggtggcg ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgacacgct
     5581 gttcgaagta acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg
     5641 caatgcagtc caccaggtcg gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga
     5701 cgcctacggc gtgggccacg ttcacggctt cagcggtgga cataatcgct gttagtgtat
     5761 ccattgacgt tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgg accagtaact
     5821 ccagtaccgc gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag
     5881 cactggcgac aagctccagc tcctgggcga aatccataat cggaaagaca gtttcaaaat
     5941 cgaagcgacg ctttagcgca gcactcattt cattgacgcc gcgatcgcgg gtattggcag
     6001 tggcaataat attaaagcct tctcgcgcat agagctgact ggcttcacca gtgagttccg
     6061 gcaccgtcat caccctgtca gaaagcatac ctaacagaca gtcttgcact tccagcggtg
     6121 tacgcgttat ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg
     6181 cagggaccag cgcttccgtt gatggaccat gattgataag cagggcgtag ttccagccgt
     6241 acttgatttg atcttcggta gtagatgcgc cgccctggat ggttaacccg gcatcgccgc
     6301 taatagaggt tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca
     6361 acatcaaacc gcgtccggtg gcaagagtga ctaacatacg ttcgacagcg gagggactaa
     6421 tcaccacttt acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag
     6481 gcaaacttaa ctgccaaccg ggcgggcaag gtgcgttatc attttgtttt aattttgcca
     6541 gttcatcggc gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca
     6601 tatattcatc cctgaaatgt attaatgaaa taaacgatat tttacgcgca ataaatgttt
     6661 ggcgcattag ttgtttgagc gaaaaatatc tggaaagcag atcacagtaa ataaaaaaaa
     6721 ctgctggtga ggcaggttct tttatttact attttcaggc ctaacaccac tgcctgtgca
     6781 acatcgtgtt ccaacgaacg aattataacc acctctggac agtgttgcaa ttaagataat
     6841 gagatttata atcctgactg ttgggaagtt gcgccggatc gaacctattt caagtcctgg
     6901 ctaccggacg ggtggtgccg caggtagtgc tctgatccgg agtgcaacat ggcgactcaa
     6961 caaacgtaaa ccagtatcga caggcagtgt aagggtaagg tgctggtgtt gtgctcctta
     7021 ataaggagag cggatggaca cgctgacaca aaagttaacc gtgctcattg ctgtactgga
     7081 gctattagtg gctctgttac ggttgattga tttgttgaag taacgggcaa attgaaacgc
     7141 atctaagggg gaataccgtt ccccccttta ataaagctat ac
//
LOCUS       DAZPDE010000179         7171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPDE010000179 DAZPDE010000000
VERSION     DAZPDE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.182"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            179..1750
                     /gene="garD"
                     /locus_tag="ACHU0J_003437"
     CDS             179..1750
                     /gene="garD"
                     /locus_tag="ACHU0J_003437"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1217978.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1802..2626)
                     /gene="agaR"
                     /locus_tag="ACHU0J_003438"
     CDS             complement(1802..2626)
                     /gene="agaR"
                     /locus_tag="ACHU0J_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1217979.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2875..4155
                     /gene="kbaZ"
                     /locus_tag="ACHU0J_003439"
     CDS             2875..4155
                     /gene="kbaZ"
                     /locus_tag="ACHU0J_003439"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1217980.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4178..4651
                     /gene="agaV"
                     /locus_tag="ACHU0J_003440"
     CDS             4178..4651
                     /gene="agaV"
                     /locus_tag="ACHU0J_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1217981.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4662..5441
                     /gene="agaW"
                     /locus_tag="ACHU0J_003441"
     CDS             4662..5441
                     /gene="agaW"
                     /locus_tag="ACHU0J_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1217982.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5431..6240
                     /gene="agaE"
                     /locus_tag="ACHU0J_003442"
     CDS             5431..6240
                     /gene="agaE"
                     /locus_tag="ACHU0J_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1217983.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6327..6761
                     /gene="agaF"
                     /locus_tag="ACHU0J_003443"
     CDS             6327..6761
                     /gene="agaF"
                     /locus_tag="ACHU0J_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1217984.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6758..>7171
                     /locus_tag="ACHU0J_003444"
     CDS             6758..>7171
                     /locus_tag="ACHU0J_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1217985.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1699 a         1864 c         1907 g         1701 t
ORIGIN      
        1 taaagcacag atttgcgctt taccttgccg ggcggcactg caatccctaa aatgattgac
       61 attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat gaaatcattt
      121 atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat attgcacaat
      181 ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag ttcacgacac
      241 agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc gttttccgga
      301 tgggctggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc tggacattcc
      361 ggctaatggt gaaattattc gttatggcga agtgattggt tacgccgtgc gtgcaatccc
      421 acgcggaagc tggatcgacg aatcaatggt tgtactaccg gaagcgccgc cgttacacac
      481 gctgccactg gcaaccaaag tcccggaacc cttaccgccg ctggaaggat acacctttga
      541 gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca tcaccaccag
      601 cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa atcattgaac gcgatctgct
      661 accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg gttgtggcgt
      721 ggcgattaac gcaccggcag cagttgtacc tattcgtacc attcacaata tttcgctgaa
      781 ccctaacttt ggcggcgaag taatggtgat tggcctgggt tgtgaaaagt tgcagcctga
      841 gcgcctgctg actggaacgg atgatgtgca agctattcca gtagaaagcg ccagcattgt
      901 cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata ttttgcaggt
      961 cgccgaacgc catctacaaa aactgaatca acgtcagcga gaaacctgtc cggcttcaga
     1021 actggttgtc ggcatgcatt gcggtggcag cgatgcgttt tctggcgtaa cggcaaaccc
     1081 ggcggttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga tgttttcaga
     1141 agtcacggaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga acgaagaggt
     1201 cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata tgggaaaaac
     1261 cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctagcaa acgtggtaga
     1321 gaaagcgctc ggctccattg ctaaatcggg caaaagtgca attgttgaag tgctgtcgcc
     1381 cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt
     1441 ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg
     1501 tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcaccccgca ccgagctggc
     1561 gaaccgctgg tttgatttaa tggatattaa tgcgggcacc atcgctaccg gcgaagaaac
     1621 tattgaagag gagggctgga agttgttcca ctttattctc gacgtcgcca gcgggaagaa
     1681 gaaaaccttc tcggatcaat ggggactgca taaccagctg gcggtgttta acccagcacc
     1741 ggtgacctga taattcaatt cgtattatgc ccgatagcac acgtttatcg ggcatattga
     1801 gtcacagcga agacgcgtga tccccgacca gaatcacttc aaccccagcc tttcgcagtc
     1861 cttccagact atccgcagga atgccttcat caacaatgat catgtcgata cgttgagtat
     1921 caatgatctt atgtaaactg gaacgattga atttactgga atcggtgacc acgatgatcc
     1981 gttccgcaac ttcgcacatc cgacggctta aacgggcttc atcttcatta tgtgtgctga
     2041 cgccgcgctc cagatcgatc gcatctacac caagaaacag catatcgaag tggtaatttt
     2101 gcagcgattg ctcagcctga tcgccgtaaa aagattgcga ctgacggcgc aaatgcccgc
     2161 cggtcatcag cagctcaacg ccttccgctt ccagcaacgc attagccacg ttcataccgt
     2221 tggtcatcgc aattacgtca gtgtacttgc gcatcagacg agcaatctca aaagtggtgg
     2281 tcccggaatc gaggatcacc cgatgacctg gctgaatcaa ctcaacggca gctttcgcaa
     2341 cgctgcgttt catcgcggtg ttcagtgcgc ttttatcttc cactgatggc tcgactgacg
     2401 gcgtcgtgct atcgcagatc aacgcgccac cataggcacg cacagcgatc ccctgctttt
     2461 ccagaaacgc cagatcattg cggatcgtca cagtagatac gccatacaat gccgacagat
     2521 cgttaacctg cacactccct tgctgtcgca gacgctgaat gatctgttct cgtcgctcgc
     2581 tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt actcatagta agtcctttcg
     2641 taaaactttc gtttcatttc gttttgccta ttaacgcctt tctattaagc aaatgcaagc
     2701 ccaccttgcc cattggcgca agctactctc gtttcagtga ctttcattac gtttcttttg
     2761 tgaatcagat cagaaaacta ttatctttcg ttttattttt atctcaccat gacgcagtat
     2821 caattgaaac aaaacgaaag atcaatatcg cagtaatctg aactggagag gaaagtgaaa
     2881 catctgacag aaatggtgag acagcacaaa gcgggcaaaa caaatgcaat ttatgccgtt
     2941 tgttccgcac atccgctggt gctggaggct gcaatccgct acgccagtgc aaaccaaacg
     3001 ccgttactga ttgaagcaac ctccaatcag gtagaccagt tcggcggtta taccggaatg
     3061 acgcccgccg attttcgcgg ctttgtttgt cagctcgccg actcgttgaa tttcccgcag
     3121 gatgcgctga ttctgggtgg tgaccatctg gggccaaacc gctggcaaaa cctgccagcc
     3181 gctcaggcaa tggccaatgc cgatgatttg attaaaagct acgttgcggc aggattcaaa
     3241 aaaatccacc ttgattgcag catgtcctgt caggacgatc caattccctt aactgatgac
     3301 atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg aagaaacctg tcgcgaacac
     3361 tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag tgccggtacc tggcggcgcg
     3421 catgaaacct taagcgagct ggcggtcacc acgccggatg ccgcccgcgc cacgctggaa
     3481 gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca tctggccacg cattattgcc
     3541 ctggtggttc aacccggcgt cgaattcgat cacaccaacg ttattgatta tcagcccgcc
     3601 aaagcgagcg ccttaagcca gatggtcgaa aactacgaaa cgctgatttt cgaagcgcac
     3661 tctaccgatt atcaaacgcc gcaatctctg cgccagctgg tgattgacca ctttgccatt
     3721 ctgaaagttg gcccggcgct gaccttcgcc ctgcgtgaag cccttttttc tctggcggcg
     3781 attgaagaag aactggtacc agcgaaagcc tgttccggtc tgcgtcaggt gctggaaaac
     3841 gtgatgctcg accgcccgga atactggcaa agccactacc acggtgacgg caacgcgcgt
     3901 cgtctggcgc gtggttacag ctactcagat cgcgtgcgct attactggcc ggacagccag
     3961 attgatgacg ctttcgctca tctggttcgt aatctggcgg attcaccaat tccgctaccg
     4021 ctcatcagcc agtatctgcc gctgcagtac gtgaaagttc gctccggcga gctgcaaccg
     4081 acgccacggg aactcattat aaaccatatt caggacatcc tggcgcagta ccacacagcc
     4141 tgtgaaggcc aataagcaaa acaaagagga acatgctatg ccaaatattg ttttaagccg
     4201 gattgatgaa cgcttgattc acggtcaggt cggcgttcag tgggtcggat ttgcgggggc
     4261 aaatctggtg ctggtagcca acgatgaggt tgccgaagat ccggtacaac aaaacctgat
     4321 ggaaatggta ctggcagaag ggatcgccgt acgtttctgg acgctgcaaa aagttatcga
     4381 caacattcat cgcgccgccg atcgacagaa aatcctgctg gtttgtaaaa cacccgccga
     4441 tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc attaacgttg gcaatatgca
     4501 ctacgccaat ggcaaacaac aaatcgccaa aacggtttct gtggatgcgg gcgatatcgc
     4561 agcatttaac gacctgaaag ccgctggggt ggaatgcttc gttcagggcg tcccgacaga
     4621 gcctgctgtg gacctcttta aattactttg agggattcat catggaaatc agcctgttgc
     4681 aggcatttgc gttgggcatt atcgccttta tcgctggcct agatatgttt aacggcttaa
     4741 cccatatgca ccgcccggtg gtcctcggcc cgttggtcgg gctggtactt ggcgatctgc
     4801 ataccggaat tttaaccggc gatacgctgg aactagtgtg gatggggctg gccccgctgg
     4861 cgggcgcaca gccgcctaac gtgattatcg gtactatcgt cggcacggcg tttgccatta
     4921 ctactggcgt gaaacccgat gtcgcggtcg gtgtcgccgt acctttcgct gtcgcagtac
     4981 agatggggat taccttcctg ttctcggtga tgtctggcgt gatgtctcgc tgcgaccgga
     5041 tggcggagaa cgccgatact cgcggcattg aacgcgtgaa ctatctggca ctgctggcac
     5101 tcggcacttt ctattttctc tgcgctttcc tgcctatcta cttcggcgcg gaacatgcaa
     5161 aaaccatcat tgatgtcctg ccgcaacgat taatcgacgg cctcggcgtc gcgggcggca
     5221 tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat gatgaaaaac gtctacatac
     5281 cctacttcat cctgggcttt gttgccgcaa cctggctcaa gttaccggtg ctggctattg
     5341 ctgccgccgc gctggcgatg gcgctgatcg atctgctgcg taaatctcct gaaccgactc
     5401 aacctgcggc acagaaagag gaatttgaag atggcatcta atcaaaccac cctgccgaac
     5461 gtcagcgaaa atgaagaaac actgctgact ggcgtcaatg aaaacgtgta tgaagatcgg
     5521 agcattgccg cggagctgac gaaaaaagat atcaaccgcg tcgcctggcg ttccatgctg
     5581 ttacaagcgt cttttaacta cgaacgtatg caggcttctg gctggttgta cggtctgctg
     5641 cctgctctga aaaagatcca cactaataaa cgcgacctgg cgcgcgccat gaaggggcat
     5701 atgggtttct tcaataccca tccgtttctg gtgacgtttg ttatcggcat tatccttgcg
     5761 atggagcgtt ctaagcagga cgttaacagt attcagagca ccaaaattgc cgtcggtgcg
     5821 ccgcttggcg ggattggcga tgcgatgttc tggctaacgc tactgccgat ttgtggcggg
     5881 ataggcgcaa gcctcgcgct acaaggctct attcttggcg cagtcgtctt tattgtgctg
     5941 ttcaacgtgg tacacttggg gctgcgtttt ggtctggcgc attatgctta ccgcatgggc
     6001 gtggcggcga ttccccttat taaagcgaat accaaaaaag tcggccatgc tgcgtcaatc
     6061 gttggaatga cggtaatcgg cgcactggtg gcaacctatg ttcgtttaag caccacgctg
     6121 gaaatcacgg cgggcgacgc agtagttaag ttacaggctg atgttatcga caaactgatg
     6181 cctgccttct taccgctggt ctacaccctg accatgttct ggctggtacg ccgcggctag
     6241 agtccgctgc gcctgattgc ggttaccgtg gttctaggga tcgtcggtaa attctgccat
     6301 ttcctttaaa tacaatgagg tattagatgt taagtattat tttgacaggg catggcgggt
     6361 ttgccagtgg catggaaaaa gcaatgaagc aaatcctcgg cgatcaatcg cagtttattg
     6421 ccatcgattt tcccgaaacc tcaagcgccg cgctgcttac atcgcagctt gaagaggcca
     6481 tcgcccaact ggattgtgaa gacggcattg tttttctgac ggatttgctg ggcggcacgc
     6541 cgtttcgcgt agcttcaacg ctggcgatgc aaaaaccggg ctgtgaagtg attaccggca
     6601 ccaatttgca actgctactg gagatggtgc tggagcgcga agggttaagc ggtgaagaat
     6661 tccgtgtaca ggcgctggag tgtggacacc gagggctgac cagtctggtg gacgagttag
     6721 gccgctgcca tgaagaatgt ccggtcgagg aaggaatatg acacacgttc tgcgcgccag
     6781 aaggctgctg actgaagagg gatggttcga tgaccatcag ttgcgtgttg ctgacggtgt
     6841 catcgcagca atcgaaccga ttccagtggg cgtgactgaa cgcgatgcgg aacggctctg
     6901 ccccgcttac atcgacaccc atgtacacgg tggtgcgggc gttgatgtaa tggatgacgc
     6961 gccggatgta ctcgacaagc tggcaatgca caaggcacgc gaaggtgtcg gcagttggtt
     7021 accgaccacc gtaaccgcgc cgcttaatac cattcatgcg gcgctgaaac gtattgctca
     7081 acgttgccaa cgcggcggac ctggtgcgca agtgctgggg agttatctcg aaggaccgta
     7141 cttcacgccg cagaataaag gcgcgcatcc g
//
LOCUS       DAZPDE010000180         7067 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPDE010000180 DAZPDE010000000
VERSION     DAZPDE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.183"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHU0J_003445"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHU0J_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHU0J_003446"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHU0J_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..>7067
                     /gene="yehK"
                     /locus_tag="ACHU0J_003447"
     CDS             6769..>7067
                     /gene="yehK"
                     /locus_tag="ACHU0J_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFR1217986.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNI"
BASE COUNT         1862 a         1824 c         1713 g         1668 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg taatagtaat ttttaattgt aaactacaga atatatt
//
LOCUS       DAZPDE010000181         6895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPDE010000181 DAZPDE010000000
VERSION     DAZPDE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.184"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..340
                     /locus_tag="ACHU0J_003448"
     CDS             <1..340
                     /locus_tag="ACHU0J_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217987.1"
                     /translation="AIVNVFKSSAVAQSTKYYEENDIHDGSNDIFFMKKYGPPAHEFV
                     RPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLNLTCWFHYKDGQWRVIT
                     YIFWPPGARF"
     gene            365..2578
                     /locus_tag="ACHU0J_003449"
     CDS             365..2578
                     /locus_tag="ACHU0J_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR1217988.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2601..3046
                     /locus_tag="ACHU0J_003450"
                     /pseudo
     CDS             2601..3046
                     /locus_tag="ACHU0J_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3068..6626
                     /locus_tag="ACHU0J_003451"
                     /pseudo
     CDS             3068..6626
                     /locus_tag="ACHU0J_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1701 a         1692 c         2063 g         1439 t
ORIGIN      
        1 agctatagtg aatgttttca agtcttctgc agttgctcaa tccaccaaat attatgaaga
       61 aaatgatata catgatggaa gtaatgacat cttttttatg aagaaatatg ggccgcctgc
      121 tcatgagttc gtgaggccac gatataaatt tgttggtgag tattatatcg gcttgaatca
      181 aacttatcgc ataacagatc ctgaatttca agatgttcct attaaggaaa tgttctggaa
      241 tttgcagggg gatttgaatc tgacatgttg gtttcattat aaagacgggc aatggagagt
      301 gattacttat attttttggc caccaggggc tagattttaa tccggtatta aaggagcaaa
      361 tatcatgagt tttgtatcta cgggtaataa accagttgga aacggtggac ccgtcatcac
      421 cacaccacca atagctggcg aaagtggcgg tatgagcaca ggttcagctg taaccgatgt
      481 atctggtgcc gccgaggaga tggcagaaca ggctgcggcg gacctgtttg gtgcgctgcc
      541 ggaaccgtca ggtctggtta aagccgcagt agctgctgcg caggctgccg ctgcagcagc
      601 agggatctcg gatatggctg gtgcagttca ggatgcagcg gcgtcgcttg cagccggtgc
      661 tccgggggcg cataacgtca cagtaagtgg tagtgcggtg ccgccgcaga tgcttttgtt
      721 tgcaggcatg aatggttcgg aagggcttgg gaatctgttt tcttacacag tacagcttaa
      781 aacgctggac gccctgaacc tggggtacgt ttcccccgct gctaacctgc cgcttcagct
      841 catggtgggc aaagacctgt gcgtcagtat cgaactggac ggcggcggaa aacggtatat
      901 cagcgggctg gtgacggcgg cgagggtggt cggccatgag agccgttcgg taacgtatga
      961 attacgcatt gagccatggc tgaagctgct gacccatacc agcgactaca aagcgttcca
     1021 gaacaaaacc gtggtggata ttctcgatga agtactggat gaatattcct ttccggtgga
     1081 aaagcggctg gtggaaaatt atccaccccg cgcctggcag gtgcagtatg gtgaaaccga
     1141 cttcgatttc atccagcgcc tgatgcagga atgggggatt tactggtggt ttgagcacag
     1201 cgagaacagc cacacgctgg tgctggtgga tgccatcaat ggtcataaag cgtgccctga
     1261 ctcgccgctg gtggagtggc accaggaagg gctgaaactg gataaggaat ttatccacac
     1321 cattacggcc agtgagaggc tgcgtactgg aaagtgggtg atggacgatt ttgattttat
     1381 gaagcctcgt tcgctgctta aaagtaccgt ggccagtccg cgtgataccg gtcatgctga
     1441 atacgagcat tacgaatggc ctggtgatta cttcactacg ggcgaaggcg agatgctgac
     1501 gcgcattcgt atggaagcgc agcgcagccc aggcagccgg gcacatgggg ccggtcatat
     1561 ccgtaccctc atgaccgggt acacctttac gttgatgaat cacccgacgg cagaaattaa
     1621 tcaggaatat ctgctggtac agaccactct gtttttacgg gataatgcgc agcacagcgg
     1681 tcaggatcag cattttacct atgtcaccac gtttgaactg caccccacca gcgaagttta
     1741 ccgcccacaa cgaaccctga gcaaacccca caccaaaggc ccgcagagcg ccatcgtcac
     1801 cggcccggcg gggcaggaaa tctggacgga taaatatggc cgggtgaagg tgcagtttgg
     1861 ctgggaccgt tacggaaaga atgacgaaaa cagttcctgc tggatccgcg tcagctatcc
     1921 gtgggcgggt aaaggattcg gcatgatcca gatcccgcgt attggccagg aagtactggt
     1981 ggatttcaaa aacggcgatc cggatttgcc catcatcgtg gggcgcacct ataaccagga
     2041 caccatgccg ccgtgggggc tgccgggcat ggcgtcgcag agtgggatat tcagccactc
     2101 attgcaggga gggccgacta acggaaatat gctgcgcttc gatgataaaa cgggtgcgga
     2161 agaggtgaag ttccatgcgg aaaaagacct caataccacg gtaaaaaaca atgaaaccca
     2221 cacggtcatg gtcgatcgca taaaaaccat tgtcaaaaat gaaaccatca gcgtgggaga
     2281 agaccgtaat gccaccatca caaaaaatga tggtctttcc gtcaaactgg ctcaaaccat
     2341 caatgtcgga acgacatatc gtctggatgt tggcgatcaa tttacccttc gttgcggcaa
     2401 tgcagcgctt gtattacaca aggatggctc gattgagttt tgcggaaaac aactgttact
     2461 gcacaccagc gatgttatgc aactcattgg cagtggcatt gatatgaatc cggatggcgg
     2521 cacagccatg actgcggatg atattgcccc gttacctacc cccgacgttt ctgaataaaa
     2581 aatataaaag gaatttttct atgaccagcc tttaccgtat tcaggagggc tgctttgccc
     2641 tgcctgaaac ttttcttgat cgcaccgtca atatcttcgt cccatcgggc aatgaaaggg
     2701 caactcccag tctgaatatt ttccgcgata cgctcaggcc agatgaaaat ctgaccacct
     2761 atattgaccg ccagatagcc ttgatgaaaa aaaaaaccta ggccagcacc gggtactttc
     2821 gcggggaacg gcactggcgg gaactggcga agctgccatg acaggtgaac agattctcgc
     2881 aactcatcgt tctggaaaaa cagaagtgta ccagcgtcag gccgggttta tcaccgggcc
     2941 agcaaaggta ctaatgttga cgctgaccac tcagcgtcct tttgatgatc acaccgacca
     3001 gctatggact gcatggttga ctagttttca gcctgctaaa agctaaaaaa aacagggagg
     3061 ttttgtcatg tatgaagccg cacgggtgga tgaccccatc taccacacca gcgcccttgc
     3121 cgggtttttg ataggggcca tcataggtat cgccattatt gccgtcgcca gctttgcttt
     3181 ttttacctgc ggctttttgg ccgggctgat tttgagtttt ctggctgatc agatagcttc
     3241 cggagtcctg caactgggtg aggctattgg gcgctcgata cattccacag tgggaaaaat
     3301 tctgaccggt tctgataatg tcagcaccaa cagtcgcccg gccgcccggg ccgtactgag
     3361 tacggtgata tgcgaagacc attccccgga aatccgcatt gcccaaggtt ccggcaacat
     3421 ctacatcaac agccagctgc ccgtaaggat gaccacactg agtgtgatgc agtgattgag
     3481 gacggctccc ccaatgtctt tctcggcggc ggtacgcaga cggtactgga aatcagcccg
     3541 gaaatccctg actggctgcg tcaggtggtg gatgtgctgt atgtggtcgc cggaatgctt
     3601 ggcgggctgg ctggcgcctg gcgtcaggcg gcaaagatgg ggtcgaaatt tggtacaaaa
     3661 tgtgcggcaa aatttattgg tggcgaaatg gtcgggatgg ccatctctga cacagtcatg
     3721 ggattgttca gcaatccggt agatgtcacc actgggcaaa aaattttgct gccggagacg
     3781 gattttaccc tgccaggacg cctgcctgtc acctgttcgc gcttttacgc cagccacctg
     3841 gaaactgaag gattgctggg cagaggatgg cggctgaact gggagataaa cctgcgtgag
     3901 gatgaaacat atatcacctt catcggtgta cagggtcggg aactgagtta tccgaaagag
     3961 atgctgatac cgggccacca gatattcgac ccggaagagc agttttacct gagccgtctg
     4021 catgacggat gctatgtgct gcattacacc gactgcagct attacgtgtt tgatgaattt
     4081 gatgaccacg gcgtcgcgcc gttacttttt atggagacgc cgtaccggca gcgtattgct
     4141 ttcgggcgtg agaacggcag gctggtgcgg gtggcctcca gcagcggaca ccatctgtta
     4201 ttgcaccgca cgatgacgca agcaggggaa cgcctgtcgc atattgaact tttgaagggc
     4261 gggcgtcccg gtaacctggt ggaatatcgt tacgacgata acgggcaact gactggcgtg
     4321 gtgaaccggg ctggtgtgac ggtgcgccag tttgcctatg aaaatggcct gatgacagag
     4381 catcgcaacg cgacgggctt tacctgtacc taccactggg aagagattga aggtttcccg
     4441 cgcgtggtgg aacacacgac cagcgacggc gaggattacc ggttccacta tgattttgct
     4501 ggcgggcaga cggtggtaac cggcaggccg gagcagaagt ggcagtggtg gtttgatgaa
     4561 gagacgtatg tgactgcgca ccggacgccg ggcggcggaa tgtaccgctt cacgtacaac
     4621 gaaaaccatt ttccggtagc ggtggaactc cccggcgaac gcagggtaac gctggaatac
     4681 gacaccctga gccgggtggt gaaagagacg gacccggcag gccgggtgac acagacgcag
     4741 tggaacggca gttttgctga aatcacgcgc cgggcgctgg atgacgacca cgtatggaag
     4801 gcggattaca acgaacacgg ccaggtgata cgggagacgg acccggaagg gcgcgtcacg
     4861 cggtacgggt atgatgatca ggggctgccg gagacggtct gccaccccgg taaacagcag
     4921 gaccgttaca cctggaacgc gctggggtta ctgagcagcc accgtcgcat cacgggcagt
     4981 gtgcagtcct ggcagtatac gcagcgcggg atgctggcgc gccatacgga tgaagagaaa
     5041 cgggagacgc gctggcagta caccccggaa gggctggttg ccagtctgag caacggcaac
     5101 ggggctcagt accggttcag ctacgacggc gacggacggc tgacgggaga acagcgcccg
     5161 gacggcctga taaggatgtt tgcgctgaat gcggacgggt ttccggtcat catcccgacg
     5221 cagggcacag agggcggggt gcgcaatgaa cagcaggagc gtgatgcgct gggccgcctg
     5281 ctgcgcagtg acacgcagca cagtacacgg accttcagct acaaccgtct tgaccagata
     5341 acggaagtga cgctcacccc cacggaagaa ggggaacggc tgcaccacat gcaggcggac
     5401 acggtgcgtt ttgcgtatga ccgcagcggg tggctgacgg cagagcacag cgtgcacggc
     5461 agtatcaaat accggcgcga tgcgctgggc aatcccaccg atatcacgtt gccggacggg
     5521 cagcatctca gtcatcttta ctacggcagc gggcatctgc tccagacggc actggacggt
     5581 atcacggtca gtgaatatga gcgtgacagc ctgcaccgtc aggtgatacg cacgcagggg
     5641 aaactggcga cgttcagcgg ctacaacgcc gacaaccggt taagctggca gcgcagcctg
     5701 ccaggtggca gccagaatcc acagcaggcg gtactccccc ggcgcagaac gacagcgtga
     5761 cggcgcggga ctatatctgg aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc
     5821 gcggttgcct ggtgttcagc tatgaccgca gcggctggct gaccagccgc acggggcaga
     5881 tgtatgacca tgaccattat tattacgaca aagcgggcaa cctgctgacg gatgagtatc
     5941 agggagcggt gatggataac cgcctgccgg gttacgggcg tgaccgctac cgctataacg
     6001 aatggggcga actgacagag cgcagggacc agcagctgga gtggaacgcg cagggccagt
     6061 taacgcgggt catcagcagc aacagcgaga cgcggtatca gtacgatgcg ctgggcaggc
     6121 gcatcagcaa ggcgaccagc aatctccata ccgaccgggg agaacgaagc cggacaacgt
     6181 ttgtgtggga agggttccgg ctgttacagg agacaacgtg gcagggaaag cggacatacc
     6241 tgtacgatgc ggaacagccg tacacgccgg tggctgccat cacggggcgc ggagagagcc
     6301 agaaaatctg gtattaccac acggacctga cgggcacggt acacgaggta accgcgccgg
     6361 acgggacgct ggtgtgggcg ggctaccagg cgggatttgg tgaaaaccgg ggcgacatca
     6421 gcaacagcgg ggcgtacttt gagcagccgc agcggctgcc ggggcagtac tttgacgaag
     6481 aaacagggct gcattacaat ctgttcaggt attatgcgcc ggagtgcggg aggttcgtaa
     6541 gccaggaccc gataggatta aacggtgggt tgaacttata tgcttatgcg cctaatccgt
     6601 taggttggat cgatccgcta ggactgaatt ctttaggaat agattcaaat aatatattta
     6661 gaggagatgg taactataaa ggtggcggaa ttggacagcc aaagagtggc acaatatcag
     6721 cacaggacat tattgatcat ctctaagaaa aaggtgatgg gcgtttaaca tcttttagta
     6781 ctaaaaaaac atctagttcc aactctcgtg gtgcagattt tttctcaaaa aaacatgtct
     6841 tcaaagtggc agtttctgat ctcgatgatt tagttaaaaa taatcagatc actgt
//
LOCUS       DAZPDE010000182         6848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPDE010000182 DAZPDE010000000
VERSION     DAZPDE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.185"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..236)
                     /locus_tag="ACHU0J_003452"
     CDS             complement(<1..236)
                     /locus_tag="ACHU0J_003452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217989.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACHU0J_003453"
                     /pseudo
     CDS             complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACHU0J_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACHU0J_003454"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACHU0J_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACHU0J_003455"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACHU0J_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1217990.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(6734..>6848)
                     /locus_tag="ACHU0J_003456"
     CDS             complement(6734..>6848)
                     /locus_tag="ACHU0J_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1217991.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
BASE COUNT         1651 a         1773 c         1784 g         1640 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgaaccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgt
     2761 atatactcgt ggcctttgcc ggaggagtca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcatt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct tcagatcgtg
     6661 aatctaaagg gttacatatt aactatattc tcaacaagac cagacactta tagaattagt
     6721 gatgatttat tcactaaaaa ggccatgaat tggaggaaaa cgatctgtcc agaaagtcta
     6781 tcggttcctc tcttattgcc tgattgtaat aatcgttatt atagtaagta ttgtttattt
     6841 catctaag
//
LOCUS       DAZPDE010000183         6839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPDE010000183 DAZPDE010000000
VERSION     DAZPDE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.186"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            278..4738
                     /gene="gltB"
                     /locus_tag="ACHU0J_003457"
     CDS             278..4738
                     /gene="gltB"
                     /locus_tag="ACHU0J_003457"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1217992.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4751..6169
                     /gene="gltD"
                     /locus_tag="ACHU0J_003458"
     CDS             4751..6169
                     /gene="gltD"
                     /locus_tag="ACHU0J_003458"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1217993.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6353..6757
                     /locus_tag="ACHU0J_003459"
                     /pseudo
     CDS             6353..6757
                     /locus_tag="ACHU0J_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT         1591 a         1863 c         1927 g         1458 t
ORIGIN      
        1 cacatttctg taccaataag cttgccattt gacctgtatc agctttcccg ataagttgga
       61 aatccgctgg aagctttctg gatgagcagc ctgctcatca tatttatgca gtaattgaga
      121 tcccctcttc accgtattaa ccgatgcgaa aaggacaaca agggggcgaa tgcgaggcgc
      181 gcgtatgaca cgcaaacccc gtcgccacgc tctttctgtg cccgtgcgca gcggttcgga
      241 agtggggttc ccgcagagcc tgggggaggt tcacgatatg ttgtacgata aatcccttga
      301 gagggataac tgtggtttcg gcctgatcgc ccacatagaa ggcgaaccta gccacaaggt
      361 agtgcgtact gcaatacacg cactggcccg catgcagcac cgtggcgcga ttctcgccga
      421 tggtaaaacc ggcgacggtt gcggcttgct gttacaaaaa ccggatcgct tttttcgcat
      481 cgttgcgcag gagcgcggct ggcgtttagc aaaaaactac gctgtcggga tgctcttcct
      541 gaataaagat cctgaactcg ccgctgccgc acgccgcatc gttgaagaag aactgcaacg
      601 cgaaaccttg tcgattgtgg gctggcgtga tgtccccact aacgaaggcg tgctgggtga
      661 aatcgccctc tcctctctgc cacgcattga gcaaattttt gtgaacgccc cggcaggctg
      721 gcgtccacgc gatatggagc gccgtctgtt tatcgcccgc cgccgcattg aaaagcgtct
      781 cgaagccgac aaagacttct acgtctgtag cctgtcgaat ctggtgaaca tctataaagg
      841 tctgtgtatg ccggcggatc tgccgcgctt ttatctggat cttgcggacc tgcgtctgga
      901 atcggccatt tgcctgttcc accagcgctt ctccactaac accgtaccgc gctggccgct
      961 ggcgcaaccg ttccgctatc tggcgcataa cggtgaaatc aacaccatca ccggtaaccg
     1021 ccaatgggcg cgtgcgcgta cctataaatt ccagacgccg ctgatccctg acctgcacga
     1081 cgccgcgcca ttcgtcaacg aaaccggttc tgactccagt tccatggata acatgctgga
     1141 actgctgctg gcaggcggga tggatatcat ccgcgccatg cgcctgttag tgccacccgc
     1201 ctggcagaac aacccggata tggacccgga actgcgtgcc ttctttgact ttaactccat
     1261 gcatatggag ccgtgggatg gtccggcggg catcgtgatg tccgatggtc gcttcgctgc
     1321 ctgtaacctc gaccgtaacg gtctgcgtcc ggcacgctac gtcattacca aagacaagct
     1381 catcacctgc gcctctgaag tcggtatctg ggattaccag cctgacgaag tggtcgaaaa
     1441 aggccgcgtc gggccaggcg aactgatggt gatcgacact cgcagtgggc gtattctgca
     1501 ctcggcagaa accgatgacg atctgaaaag ccgccaccct tataaagagt ggatggagaa
     1561 aaacgtccgc cgactggtac cgtttgaaga tctgcccgat gaagaagtgg gtagccgcga
     1621 actggacgac gacacgcttg ccagctacca gaaacagttt aactacagcg cggaagagct
     1681 ggactccgta attcgcgtac tgggcgaaaa cggtcaggaa gcggtcggtt cgatgggcga
     1741 tgatacccca ttcgccgtgc tctccagtca gccgcgcatt atttacgact acttccgcca
     1801 gcagtttgcc caggtgacta acccgccaat cgacccgctg cgtgaagcgc atgttatgtc
     1861 gctcgccacc agtatcggtc gtgaaatgaa cgtcttttgc gaagcagagg gccaggcgca
     1921 ccgtttaagc tttaaatcgc cgattctgct ctactccgat ttcaaacagc tcacgacgat
     1981 gaaagaggag cactaccgcg cagatacgct ggatatcacc tttgacgtca ctaaaaccac
     2041 gctcgaagcg acagtcaaag agctatgcga caaagccgaa aaaatggtac gtagcggcac
     2101 cgtgttgctg gtgctctccg accggaatat cgctaaagat cgcctgccgg ttccggctcc
     2161 gatggcggtt ggcgcgatcc agacccgtct ggtcgatcaa agcctgcgtt gcgatgccaa
     2221 catcatcgtc gaaaccgcca gcgcccgcga tccgcaccac ttcgccgtgt tgctgggttt
     2281 cggcgcgacg gctatttatc cgtaccttgc ctatgaaacg ctgggccgcc tggtagacac
     2341 ccacgcgatt gccaaagatt atcgtaccgt gatgctcaac taccgtaacg gcatcaacaa
     2401 aggcttgtac aaaatcatgt ccaaaatggg catctccacc atcgcctctt accgctgctc
     2461 gaaactgttt gaagcggtcg gtctacacga tgatgtagtg ggcctgtgct tccagggcgc
     2521 ggtcagccgc attggtgggg caagctttga agacttccag caggatctgc tgaatctgtc
     2581 gaaacgtgcc tggctggcgc gcaagccgat tagccagggc ggcctgctga aatacgtcca
     2641 cggcggcgaa taccacgcct acaacccgga cgtggtgcgc acgctgcaac aggcggtaca
     2701 aagcggcgag tacagcgact atcaggaata cgcgaagctg gttaatgagc gtccggcaac
     2761 cacgctgcgc gatctgctgg caattacgcc gggtgaaaac gcggtcaaca ttgctgatgt
     2821 tgaaccggca agcgaactgt ttaaacgctt tgataccgcc gcgatgtcta tcggcgcgtt
     2881 aagcccggaa gcccacgagg cgctggcgga agcgatgaat agcatcggcg gtaactcgaa
     2941 ctccggtgaa ggcggcgaag acccggcgcg ctatggcact aacaaagtgt cgcgcatcaa
     3001 gcaggtggct tctggtcgct tcggcgttac tccggcgtat ctggtcaatg ccgacgtcat
     3061 tcagattaaa gtcgcccagg gcgcgaagcc gggcgaaggt ggtcagttgc cgggcgacaa
     3121 agtcactcct tacatcgcca aactgcgcta ttcggtgccc ggagtgacgc tgatctcccc
     3181 gccgccgcac cacgatatct actctatcga agacttagcg cagcttattt tcgacctcaa
     3241 gcaggttaac ccgaaagcga tgatctccgt taagctggtg tccgaaccag gcgttggcac
     3301 catcgcgact ggcgtggcga aagcttatgc ggacttgatc accatcgcgg gctatgacgg
     3361 cggcaccggc gcaagcccgc tgtcgtcagt gaaatatgca ggctgcccgt gggaactcgg
     3421 tctggtggaa acccaacagg cgctggttgc caacggcttg cgtcataaaa ttcgtttgca
     3481 ggttgatggc ggcctgaaaa cgggtgtcga tatcatcaaa gcggcgattc tcggcgcaga
     3541 aagcttcggc ttcggcactg gcccaatggt ggcgctcggc tgtaaatatc tgcgtatttg
     3601 ccacctgaac aactgcgcca cgggtgtagc aactcaggat gacaaactgc gtaagaatca
     3661 ctatcacggc ctgccgttca aggtgactaa ttactttgag tttatcgccc gtgaaacccg
     3721 cgagctgatg gcacagctgg gagtaacgcg tctggtggat ctgattggtc gcaccgacct
     3781 gctgaaagag ctggacggtt tcaccgccaa acagcagaaa ctggcgctgt cgaagctgct
     3841 ggagactgcc gagccacatg caggtaagtc actctactgc actgaaaaca acccgccgtt
     3901 tgataacggc ctactgaacg cgcagttgct gcaacaggcg aaaccgtttg tcgatgaacg
     3961 ccagagcaaa accttctggt tcgatatccg caacaccgac cgttctgtcg gcgcgtcgct
     4021 ttcaggctat atcgcccaga cgcacggcga tcaggggctg gcagccgatc ctatcaaagc
     4081 gtacttcaac ggcaccgcag gccagagttt cggtgtgtgg aacgcgggcg gcgtggaact
     4141 gtacctgacc ggggatgcca acgactatgt cggtaaaggc atggcgggcg gcttaatcgc
     4201 cattcgtcct ccggttggtt ccgccttccg cagccatgaa gcaagcatta tcggcaacac
     4261 ctgcctgtat ggcgcgaccg gtggtcgtct gtatgccgca ggccgcgcgg gtgaacgttt
     4321 cggcgtgcgg aactccggtg ctatcaccgt ggtagaaggc attggcgaca acggctgtga
     4381 atatatgacg ggcggtattg tctgtattct gggtaaaacc ggcgttaact tcggcgcggg
     4441 catgaccggc ggcttcgctt acgttctcga tgaaagcggt gatttccgca aacgcgttaa
     4501 cccggaactg gtcgaagtct taagcgtcga cgatctggcg atccatgaag agcatctgcg
     4561 cggtcttatc accgagcatg ttcagcatac cggttctcag cgcggtgaag agattctggc
     4621 gaactggtca accttcgcca cgaaattcgc gctggttaaa ccgaagtcca gtgatgtaaa
     4681 agcactgctg ggtcaccgta gtcgtagcgc agcagagttg cgcgtgcagg cgcagtaagg
     4741 ggtagcaaca atgagtcaga atgtttatca atttatcgac ctgcagcgcg ttgatccgcc
     4801 aaagaaaccg ctgaagatcc gtaaaattga gtttgttgaa atttacgagc cgttttccga
     4861 aggccaggcc aaagcgcagg ctgaccgctg tctgtcgtgc ggcaacccat actgcgagtg
     4921 gaaatgcccg gtacacaact acatcccaaa ctggctgaag ctcgccaacg aggggcgtat
     4981 ttttgaagcg gcagaactgt cgcatcagac caacacactg ccggaagtat gcggacgtgt
     5041 ctgcccgcaa gaccgtctgt gcgaaggttc ctgcaccctg aacgatgagt tcggtgcggt
     5101 gaccatcggc aacattgagc gttatatcaa tgataaagcg ttcgaaatgg gctggcgtcc
     5161 ggatatgtcc ggcgtgaagc aaaccggtaa aaaagtggcg attatcggtg caggcccggc
     5221 aggtctggcg tgtgcggatg tcctgacgcg taacggcgta aaagccgttg tcttcgaccg
     5281 tcatccagaa attggcgggc tgctgacctt cggtattccg gccttcaagc tggaaaaaga
     5341 ggtaatgacg cgtcgccgtg aaatcttcac cggcatgggt attgaattca aactcaatac
     5401 cgaagtgggc cgcgacgtac agctggacga tctgctgagt gattacgatg ccgtgttcct
     5461 tggcgttggg acttatcagt caatgcgcgg cgggctggaa aacgaagacg ccgatggcgt
     5521 gtacgcagcg ctgccgttcc ttatcgccaa caccaaacag ttaatgggct ttggtgaaac
     5581 ccgcgacgaa ccgttcgtca gcatggaagg caaacgcgtg gtagtacttg gcggtggcga
     5641 cactgcgatg gactgcgtgc gcacgtctgt acgccagggg gcaaagcacg ttacctgtgc
     5701 ctatcgtcgt gatgaagaga acatgccggg ttcccgccgc gaagtgaaaa acgcgcggga
     5761 agaaggcgta gagttcaaat tcaacgtcca gccactgggg attgaagtga acggtaacgg
     5821 caaagtcagc ggcgtaaaaa tggtacgtac cgaaatgggc gaaccggatg ccaaaggccg
     5881 tcgccgcgcg gagatcgttg caggttccga acatatcgtt ccggcagatg cggtgatcat
     5941 ggcgtttggt ttccgtccac acaacatgga atggctggca aaacacagcg tcgagctgga
     6001 ttcacaaggc cgcatcatcg ctccggaagg caacgacaac gctttccaga ccagcaaccc
     6061 gaaaatcttt gctggcggcg atatcgtccg tggttccgat ctggtcgtta ccgccatcgc
     6121 cgaaggtcgc aaagcggcag acggcattat gaactggctg gaagtttaag ctctgtccca
     6181 gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc tttctttctg gaaatcgcct
     6241 cgcataatct gaagaaaacc aatgtaatac gctcatctgg attgttaccg gtagatgccc
     6301 ggcgtatagc tgattccatt attcaacgca gcgccatata aagggacaga taatggaatc
     6361 gctctcagag agaacctcaa caggctacca gcaaatccac aacggcatta ttcatctggt
     6421 cgatagcgcc cgaacggaaa cggtacgtag cgttaacgcg ctgatgaccg cgacgtactg
     6481 ggaaattggc cgacggattg tcgaatttga acaaggtggt gaggccagag cagcttatgg
     6541 tgcacagttg attgatcggc tatcgaagga tttaagtcga cggtataaac gtggggtctc
     6601 tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt tttcaacatg ttgaaattcg
     6661 ccagacagtg tctggcgaat taacaccatt gggaattccg cagacaccat ctgcagaatt
     6721 cccatcggca aagatttggc agacgctgtc tgccaaattg agaggctgtt tgatatttcc
     6781 acagcttttg tattgcatct cctattttcg gtttgtatat tcatgcaaat aaacgaagg
//
LOCUS       DAZPDE010000184         6716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPDE010000184 DAZPDE010000000
VERSION     DAZPDE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.188"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..106)
                     /locus_tag="ACHU0J_003460"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="ACHU0J_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            104..428
                     /locus_tag="ACHU0J_003461"
                     /pseudo
     CDS             104..428
                     /locus_tag="ACHU0J_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(458..3370)
                     /locus_tag="ACHU0J_003462"
     CDS             complement(458..3370)
                     /locus_tag="ACHU0J_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR1217994.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3853..4695
                     /locus_tag="ACHU0J_003463"
     CDS             3853..4695
                     /locus_tag="ACHU0J_003463"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1217995.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4698..5786
                     /gene="capU"
                     /locus_tag="ACHU0J_003464"
     CDS             4698..5786
                     /gene="capU"
                     /locus_tag="ACHU0J_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR1217996.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5791..>6716
                     /gene="virK"
                     /locus_tag="ACHU0J_003465"
     CDS             5791..>6716
                     /gene="virK"
                     /locus_tag="ACHU0J_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1217997.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPV"
BASE COUNT         1786 a         1544 c         1596 g         1790 t
ORIGIN      
        1 cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc catgaaagcc
       61 ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc agcggcggac
      121 agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt acccggtcaa
      181 aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt taccacttta
      241 acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag gcctcccgga
      301 agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc
      361 aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg tacttacgta
      421 cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga gcggcacgac
      481 ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc
      541 ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc aaatcatgcc
      601 ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc ttccttattt
      661 taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc ttttttccat
      721 aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa ctccttcgtt
      781 tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac acactctgcc agtcgtgccg
      841 cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac agcgcctgca
      901 tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg cgtccttcca
      961 cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg
     1021 cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc agcccgtcat
     1081 taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt gcaatattac
     1141 ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga tcaacaccat
     1201 aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc cggaaatact
     1261 tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta aaatcttccc
     1321 ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc agcgcctgca
     1381 gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt cgtgcatcct
     1441 ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta cggatggtgt
     1501 ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc cactcatcac
     1561 ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc gcaagcagac
     1621 tccacagagg atgctccacc tgctgttcaa caagatacac attgtgttta gcacgggtga
     1681 gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt
     1741 cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa
     1801 tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc gtttcgtatt
     1861 ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag catcgcgcct
     1921 gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt gaggttttgg
     1981 cattcagttc ctgctttgtc tcttccctat cgtccagcaa ccggatggtc ccttctgcct
     2041 gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag cggacctgtt
     2101 tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg taattcgcct
     2161 gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga cttttgagcg
     2221 aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag agtaaaaact
     2281 gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc atatcctgaa
     2341 cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac tgatgactga
     2401 gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt tcaaacagga
     2461 catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc agtgacagat
     2521 acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg cccctgaact
     2581 cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg ggcagccagt
     2641 cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt tccagaaatt
     2701 ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa gcgtcataga
     2761 gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata tcaccagcag
     2821 cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag ccaacaatca
     2881 ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac agcattttat
     2941 ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca gtcgcgatat
     3001 caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc acgtttctgc
     3061 gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca aggcagatag
     3121 tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg agccgggcgc
     3181 ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg gtggcaatag
     3241 cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga taaagacaga
     3301 ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga aggaatgcct
     3361 tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca gaaaaatgct
     3421 ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc gctggaaatg
     3481 gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa
     3541 agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg agtacctgtg
     3601 ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt gccacctcaa
     3661 ggttcttagt gagtcgtttt taaaagatat gataatatat cattgtttat gaattgttga
     3721 tgtttaattt agtaaattat atcaaatatg ccatgggttt gctatgttat tcatccagta
     3781 tctggaaata aaatgcacca gctttattgg gttttccttt tgtctctgct tgctaggtgc
     3841 cagaaacttt aaatgttaaa cgaagggggc attttgttca aagccaatca tgttccggta
     3901 ttaatgtatc atcatgtcag tcattgcccc ggactggtta cactttctcc cgtgacattc
     3961 cggaagcaaa taaaatggtt ggctgaaaac aactggaaaa cactgtcatc agatgaactg
     4021 gagttttttt atcggggcgg aaaactcccc cggaaaagcg ttatgttgac atttgatgat
     4081 ggataccttg acaactggtt tcaggtatat cctttattaa aagagtttaa tctcaaagca
     4141 catatttttt taattaccgg ttttattggg aatggtccgg ttcgtcactc tcctggaaag
     4201 gaatattctc atcgcgattg cgaacatcaa atagcgacag ggaatgctga taatgttatg
     4261 cttcgctggt ctgaagtaaa cgaaatgttg caatctggtc tggtcgaatt tcatgttcat
     4321 acgcatactc atacacgctg ggataaaaag ttcagttcac gagaagaaca gtgtaaacat
     4381 cttcggcagg acttgttgag cgggagggaa tatttgaaag aaatgacagg aaaatgcagt
     4441 aaacatttgt gttggccgga aggttattat aacaaggatt atattcaggt cgctgaagaa
     4501 cttggattct attatttata tacaacagaa agaagaatga atgctcctgc taaaggaact
     4561 acacggatag gaagaataag caccaaagaa cgggaaagtt gcgcatggct gaaaagacga
     4621 cttttttatt atacaacccc gtttttttca tcgttgctgg cctttcataa ggggccaaga
     4681 ttaccggatg attaataatg aatatactat ttacggaatc atcaccgaat ataggtggtc
     4741 aggaattaca ggctgttgct caaatgaagg ccctgaagaa aatggggcat tcagttctgc
     4801 ttgtctgcag ggaaaacagc aaaattgctt ttgaagccag taaattgggg attgatatca
     4861 cattcgcgtt atttcgaaac agtcttcaca tccctactgc atggagatta ctcggaatag
     4921 ttcatggttt tcagcccaat gcaatcgttt gtcacagtgg gcatgatagc aatattgttg
     4981 gtttagtacg gttattcact aggaaacatc cattcagaat tatcaggcaa aagacatatt
     5041 tgacacgaaa aacaaaagtt ttttcaataa atcatttttg cgatgaggtg attgttcccg
     5101 gaacaagtat gaagacacat ctggagcagg aaggatgtcg aacccgggtt actgttgtgc
     5161 ctccaggctt tgatttccag aaattatacg ttgattcccg aaacagtttg cctccaaatg
     5221 ttctttcttg gctggcgtcc cgaaggggat gccccgttat tgctcaggta ggaatgttgc
     5281 gcccggaaaa agggcacgaa tttatgttga atttactgtt ccatttaaaa atgaatggac
     5341 gacagttctg ttggttgatt gtggggtctg gttcgcctga actgcgggag catttacagt
     5401 atcagattga cagtatgggc atgcatgatg atgtttttat tgctgacaat gtttttcctg
     5461 ccgcccccgt atatcgggtt gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg
     5521 gtatggtact ggcagaagca tcggcatttt ctgtgcctgt actggccagt cagattggtg
     5581 gaatccctga tgttattcag aataaccaga ccgggacatt gttaccagca ggtaataagc
     5641 acgcatggat gtgcgccctg aatgattttt ttaatgaccc tgggcgtttt tatcagatgg
     5701 ctcgccaggc aaaacaggat atagaagagc ggtttgatat taataaaact gcgttaaaaa
     5761 tactcacatt agcgaagcac aagtaacaat atgttttctg taagtaactt atcatttatc
     5821 ggtttcctta aaaggattgt tttttcctca gattcactcc cggggaagtg ggaacacaga
     5881 aaattccggt tcatgtacat tttgcgatgt gctataaatc cggttgccag tattcgatat
     5941 tattacgaac tgcgttcctt gcagtgcatt gaggatattc tggcaataca acccacgttg
     6001 ccagcaagaa ttcatcgacc ttatttgcat aaaggggggc gagcctggtc ccgggggcaa
     6061 tatattcttg agcattatcg ctttgtgcaa aatttgccag aaaaatattc tgaatttttg
     6121 tttccacaaa aatcggtatc attagtccaa ttcatcggga aagatggaga ggattttgat
     6181 atccagtgtt ctcccagtgg ttttgaccgg gaaggagagt tgatgttgtc attgtttttc
     6241 aataaaatag tgattgcccg gttaacattt tcagtcattc tgactcaaaa tggacacacc
     6301 gcttttattg gcggattaca gggagccccc aaaaataccg gacctgatat tattcggtgc
     6361 gcaacccggg cctgttatgg gcttttccca aaacggatta tttttgaagc attttgtgcg
     6421 ttgatgaaag cttgtaatgt ctctgagtgt ctggcagtca gtgagcacag tcatgttttc
     6481 cggcaattga gatactggta tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc
     6541 tgggagtctg tagcagggaa aacctgtggt gactggtaca aattaccaac acaggtggtt
     6601 cgaaagccgc taagcaatat agctagtaaa aaacgctctg agtatcgaaa acgatacgct
     6661 ttgctggatt atatccatga aactgctatc cgctctttgg atgcatatcc tgtaaa
//
LOCUS       DAZPDE010000185         6696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPDE010000185 DAZPDE010000000
VERSION     DAZPDE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.189"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            100..354
                     /gene="bssS"
                     /locus_tag="ACHU0J_003466"
     CDS             100..354
                     /gene="bssS"
                     /locus_tag="ACHU0J_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1217998.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            469..1587
                     /gene="solA"
                     /locus_tag="ACHU0J_003467"
     CDS             469..1587
                     /gene="solA"
                     /locus_tag="ACHU0J_003467"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1217999.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1635..1748
                     /locus_tag="ACHU0J_003468"
     CDS             1635..1748
                     /locus_tag="ACHU0J_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR1218000.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2009..2575
                     /locus_tag="ACHU0J_003469"
     CDS             2009..2575
                     /locus_tag="ACHU0J_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1218001.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2579..3154
                     /locus_tag="ACHU0J_003470"
     CDS             2579..3154
                     /locus_tag="ACHU0J_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1218002.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3196..4248)
                     /locus_tag="ACHU0J_003471"
     CDS             complement(3196..4248)
                     /locus_tag="ACHU0J_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1218003.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4473..5393
                     /gene="lpxL"
                     /locus_tag="ACHU0J_003472"
     CDS             4473..5393
                     /gene="lpxL"
                     /locus_tag="ACHU0J_003472"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1218004.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5565..5750
                     /locus_tag="ACHU0J_003473"
                     /pseudo
     CDS             5565..5750
                     /locus_tag="ACHU0J_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5786..>6696
                     /locus_tag="ACHU0J_003474"
     CDS             5786..>6696
                     /locus_tag="ACHU0J_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1218005.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT         1722 a         1605 c         1604 g         1765 t
ORIGIN      
        1 ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct
       61 cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc
      121 attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg
      181 atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt
      241 cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga
      301 atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac
      361 atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct
      421 gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat
      481 ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc
      541 ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac
      601 ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc
      661 ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt
      721 gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc
      781 cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc
      841 cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt
      901 tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt
      961 gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt
     1021 gaaacggttg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta
     1081 aaagacctgc tcccggagct gcctgtccag cccgtacgta aagtatttgc ctggtatcag
     1141 gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat
     1201 ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac
     1261 ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggccagtgat
     1321 gggtcagaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac
     1381 ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc
     1441 ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca
     1501 gttttagggg aaatagctgc cgattttgcg caagaccaaa aaagcgattt tgatttgacg
     1561 ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt
     1621 tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc
     1681 tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt
     1741 atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt tcatttagcg
     1801 tgaaaaattg aatgagatta ttcctgcttc tacagcactc accagacatc tctgatgctg
     1861 aacccactaa acttatgaag attctttcat tattaattca aaattattga aataaaaata
     1921 aatatcattc aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt
     1981 ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca aatacccctg aacgctatgg
     2041 tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt
     2101 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
     2161 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt
     2221 catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa
     2281 agctggacat ctcgcattgt gcctattgct gttcgccatc ggcatcagcg gctatctgat
     2341 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
     2401 tgccgacgct ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag
     2461 cgtcgtagta ctgtctgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa
     2521 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
     2581 gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt
     2641 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcataca
     2701 gcatcttggc tatagctggt tatatggcac ctttaaagat ttcgacggta cttttacctt
     2761 tgatgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
     2821 tactaaccac gccgaacgcg ataaacatct tcgcagtgcg gattttctca ataccacaaa
     2881 atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat
     2941 taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat
     3001 tggtcagggc gacgacccat ggggcggtaa acgtgcaggc tttgaggccg aaggcaaaat
     3061 taagctcaaa gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct
     3121 gattatttca gtggaaggcg tacagcagaa gtaagagtga aatgttgccg gatggcacgc
     3181 atccggcaat gatatttatt ctgttggatc aggaataccc agtgttgtat tcagacgtcc
     3241 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
     3301 ttctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
     3361 cgcgcatact ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg
     3421 gctgtcgcac ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc
     3481 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc
     3541 gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc
     3601 gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacatgac
     3661 gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa
     3721 ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc acttcatact catagtggtt
     3781 acgcatgtcg ataaatagtg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg
     3841 cagatactca ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt cggcaacaat
     3901 gcgatcgcgt accttcatgc gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat
     3961 attcaggcgt aaaccctcta aagccgggtc gaaggcatag agctgcgcgc gaaatgtttc
     4021 aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg
     4081 cccaaaaaca ttcagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc
     4141 ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct cagccaacat
     4201 tttggctttt aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc
     4261 ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc
     4321 cacacatttt acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat
     4381 ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca
     4441 attgcccgta taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg
     4501 cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttggtcg
     4561 tgcaattgcc ctacccgatt atctaccgcc tcggttgtgg attaggaaaa ctggcgttac
     4621 gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa
     4681 tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggcc
     4741 tgatggaaac cggtatggcg tggttctggc cggaccgccg aatcgcccgc tggacggaag
     4801 tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg
     4861 gcatccattt tctgacactg gagctgggcg cgcggcagtt tggtatgcag gaaccgggga
     4921 ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt
     4981 tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga
     5041 aaaaaggcga agtggtctgg tacgcaccag atcatgatta cggcccgcgc tcaagcgttt
     5101 tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac cggaacctgg atgctggcac
     5161 ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt
     5221 atcaattgat tatgctgcca ccagagtgtt ctccgccact ggatgatgcc gaaactaccg
     5281 ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt
     5341 ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc
     5401 atgccggatg cttcagaatg gcatccggca ttaccacagc aaatccccct gatttagcga
     5461 taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt gtttatcttt
     5521 tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa
     5581 atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta
     5641 ccggggccgc cttcagtctg gtaatgccct tcttacccct ctacgttgag cagcttggtg
     5701 ttaccggcca ctccgccctg aatatgtggt ccggtattgt cttcagtata atgggttcct
     5761 aaacacatca ttttaggtat tgaatatgag acgggcaatc agctacatca gattttcaag
     5821 tgaacgacaa ctgaaagggg actctgtacg ccgacaatct aagctggtta ctgactggct
     5881 tgataagaac cctgagtttt acttggattc atccttgagc tttaaagatt tggggaaaag
     5941 tgcttttagt ggtaagcacc tgaaaggtgg tctaggtgac tttttaaccg ctatcgagaa
     6001 agggcttgtt aaagctggtg atacgctact aatcgagagt ttagaccgtc tgtcacgtca
     6061 ggatattgat attgcttctg agctattacg ccgtatctta agggctggtg tagatgtagt
     6121 cacactgagc gatggagaac attacacacg agaaagttta aaagacccgt tagccctgat
     6181 taaaagcatt ctgataatgc agcgggcgca tgaggaaagt ttgagaaagt ctgagcgtgt
     6241 ccaagctgca tggaaccgta aaaaagaact catctccgaa ggtatcaagg tatctaggcg
     6301 ttgtcctgca tggttaaggc tgaatgatga ccgtcgaaca ttcacaatca ttcctgataa
     6361 agtggaagtt gttaaacgag catttgacct gagattacag ggtttaagct tttgggctat
     6421 caccagaact ttaaacgatg aaggtcactt gtcattgaat caatacacac ccaaacaaaa
     6481 ggggtggtct gatactgctg taaagaaact gctcagaaat cgtgctgtga ttggatgttt
     6541 cacacctgct ggacgtgaag aagttcaggg ttactacccc gcaataatca gtgagtcact
     6601 attctatcga gtacagcaac tgaacacagg gcaatatggt agggcttctg tatcaagtaa
     6661 tccgctatca gtcaacttgt tcaggggtat catcaa
//
LOCUS       DAZPDE010000186         6685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPDE010000186 DAZPDE010000000
VERSION     DAZPDE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.190"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..430
                     /locus_tag="ACHU0J_003475"
     CDS             <1..430
                     /locus_tag="ACHU0J_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218006.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="ACHU0J_003476"
     CDS             550..723
                     /locus_tag="ACHU0J_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR1218007.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="ACHU0J_003477"
     CDS             823..1644
                     /locus_tag="ACHU0J_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1218008.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            1986..2459
                     /locus_tag="ACHU0J_003478"
     CDS             1986..2459
                     /locus_tag="ACHU0J_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1218009.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2475..2951
                     /locus_tag="ACHU0J_003479"
     CDS             2475..2951
                     /locus_tag="ACHU0J_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR1218010.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3014..3235
                     /locus_tag="ACHU0J_003480"
     CDS             3014..3235
                     /locus_tag="ACHU0J_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR1218011.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3398..3772
                     /locus_tag="ACHU0J_003481"
     CDS             3398..3772
                     /locus_tag="ACHU0J_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR1218012.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            3819..4193
                     /locus_tag="ACHU0J_003482"
     CDS             3819..4193
                     /locus_tag="ACHU0J_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR1218013.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            4190..4681
                     /locus_tag="ACHU0J_003483"
     CDS             4190..4681
                     /locus_tag="ACHU0J_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR1218014.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            4693..4890
                     /locus_tag="ACHU0J_003484"
     CDS             4693..4890
                     /locus_tag="ACHU0J_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR1218015.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            4975..5535
                     /locus_tag="ACHU0J_003485"
                     /pseudo
     CDS             4975..5535
                     /locus_tag="ACHU0J_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            5607..6611
                     /locus_tag="ACHU0J_003486"
     CDS             5607..6611
                     /locus_tag="ACHU0J_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR1218016.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
BASE COUNT         1662 a         1763 c         1674 g         1586 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctgaggcagc atgtccctga aggcgtgatt caggcaccag
     1681 aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat gatgacctgt
     1741 gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc agactgagta
     1801 tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1861 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg tcagtgatga
     1921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     1981 catacatgaa aacattgtct cagaacacca ccgcttcggc ctgtgcaccg gagactggcc
     2041 tgcaacaact ggttgccact cccgtccctg atgaacagcg catcagcttc tggccgcagc
     2101 attttggcct cattccacag tgggtcaccc tggaaccccg tgtcttcggc tggatggacc
     2161 gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     2221 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     2281 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc tgtctgatga
     2341 cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat tattaccggc
     2401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     2461 tggaggcagg gacaatgcag cagctttcct ttctgcccgg aaacatgacg cccggcgagc
     2521 gcagtctcat tctgcgggcc ctgcaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     2581 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
     2641 gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     2701 tcttcaccgg caccatcaac cgtacggaag ttcatccccg ggaagtgatt aaacgcgccc
     2761 tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     2821 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     2881 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     2941 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     3001 gaggtatcac atcatgagaa tcatcacccg tggtgaagcc atgcgtattc accaacagca
     3061 tcctgcatcc cgtctttttc cgttctgtac cggtaagtat cgctggcacg gcagtgctga
     3121 agcgtatact ggtcgtgaag tgcaggatat tcccggcgtt cttgccgtgt ttgcagaacg
     3181 ccgtaaggac agttttggcc cgtatgtccg gctgatgagt gtcaccctga actgatgttc
     3241 cgtcacccgg gaaacgagta cgaccagatg acctcagacg ccgctgtgga tgaggccatt
     3301 ctcatcaatg catacatctt caccgaaacc ggtcagcgcg ccggctgacc tgtcatcgtt
     3361 aaaccattct taaccacttt tcagagagga ttttatcgtg tcagacaaac tctccgggat
     3421 aactcatccc gatgacaatc acgaccgccc ctggtggggg cttccctgca cagtgaggcc
     3481 ctgttttggc gctcgtctgg tgcaagaggg taaccgcctg tattaccttg ccgaccgcgc
     3541 cggtatcaga ggccgcttca gcgacgcaga tgcataccat ctggaccagg cctttccgct
     3601 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gatatcagca
     3661 taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg
     3721 ttacgtttat ctggctgttt atccgacacc agcacccgca accacctcat aaccccgaag
     3781 ctctcacctt tagcactacc agttacagag aacttaagat gaaaacatta tccgacacac
     3841 atgtacggga ggtatctcgc tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc
     3901 gactgctgga ccagcattat ggcctcacac tgaatgacac accgtttgtc gatgaacgtg
     3961 tgattgagca gcatattgag gcaggcattt cactgtgtga tgcagttaat tttctcgtgg
     4021 aaaaatacgc gctggtgcgt accgaccagc cgggattcag cacctgtccc cgctctcagt
     4081 taataaacag tattgatatc ctccgggctc gccgggcgac cggcctgatg acccgcgaca
     4141 attacagaac ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgaaact
     4201 ggccctgaca ctggaagccg acagcgttaa cgtacaggca ctgaacatag ggcgcattgt
     4261 cgttgacgtc gatggtattg agctcgctga actgattaac atggtctgcg ataacggcta
     4321 ctcccttcgt gttgttgatg aatctgacca gacctcagca gaatgcacgc caccatttgc
     4381 tacccttacc ggcatacgct gcagtaccgc acatatcacg gaaacggaca acgcctggtt
     4441 gtactcgctg tcacaccaga ccagtgactt cggtgaatca gaatggattc attttacagg
     4501 tagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaaacgcct
     4561 ggggctgtca aaaacgttcc gtcgtctggt cgtcacactc atccggcgtt atggcgtcag
     4621 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     4681 accaggaaca acatgaaatc attaaccacg gaaacagcgc tggatattct gattgcgtgg
     4741 ctgcaggaca atatcgactg cggatcggga attatctttg acaacgatga ggataaaacg
     4801 gattcagcag cactgttgcc ctgtatcgaa caggcccggg aggatgtccg taccctgcgt
     4861 catctgcagc ttctgcacca gaaccggtga agctcactca ccagacttca ttcctctgac
     4921 gccagcctgc acagggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtca
     4981 gatatcacaa tctcccgtcc ggaagtggtc aacgggcata cggacgttat ctgctcaacc
     5041 agcattcgcc acattctggc tgtacgaaag agtacactgt tgcaaatcga cacacttatc
     5101 cgacaactgg ctgaaatctc ggtgctgacg gaaagcatcg gaggtaaaac tgccccggac
     5161 tgggccatga aacaggattt tcgctgcggc tgctggctga tggagaaacc cgaaacagca
     5221 atgaaagcca ttacccggaa tcttgatcgc gaaatctggc gtgacctgat gcaacgttcc
     5281 gggatgctgt cattaatgga tacgcaggcc cgtgatacat ggtaccggtc actggagtac
     5341 gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca actgcaccag
     5401 aataaggatg aggtgtttga gcgaggggtg atcaacgtct tcagagggct gaactggaat
     5461 tacaaaacca attgcccctg taaatttggc agtaaaatta tcgtcaacaa tctggtgaga
     5521 tgggaccggt ggggataatg atatggtcac ccccacctta acagcagtac gctgttaagg
     5581 taaatccctt taacggagag accatcatgc agaacatcac acttattggt atcgatctcg
     5641 gaaagcactc cttccacatt cattgtcaag acaagtctgg taaggctctt ctgcgtaaaa
     5701 aatttacccg tactaaactg atgcagtttc tggcgacatg tccgccctca gttgttgtaa
     5761 tggaagcctg tgcgggtgca cactttatgg ctcgctgtat ctctgatttt gggcatgaaa
     5821 ctaagctgat ttctccacag ttcgttcgcc cgtttgtcaa aagtaataag aatgattttg
     5881 ttgatgcaga agccatatgc gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
     5941 gaactgagac ccagcaggcc atgagggcgt tacaccgggt cagagaatcg ctcatcagag
     6001 ataaggtgaa aacaaccaac cagatccacg gtttcttgct ggaatttgga atcagcctgc
     6061 cgataggtga tgctgtaata aaacggttgt ctctcgtgtt ggctgaacac gaaatccccg
     6121 aatatctgag ccgccttttg gtgaggttgc atacccacta cttttacctt gttgagcaaa
     6181 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
     6241 ttatgacaat tcctggtgtt ggtccgatta ctgccagctt actatcgtcg caacttggtg
     6301 acgggaaaca gttctcatgc agcagggatt ttgcagcttc cacaggactt gttccccggc
     6361 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
     6421 tacgccggtt actggttcag tgtgcccgct cattcataat gcgacttgaa cgcaagcatg
     6481 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttacct
     6541 gcgctctggc aaataaactg gctcgaattg cctgggcaat cactactcag aaaaaagaat
     6601 atcaggcctg atatagatgc tgatataact caattagttt attttaaaca ataagataca
     6661 agttaccttt ctggttttgc gaaga
//
LOCUS       DAZPDE010000187         6597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPDE010000187 DAZPDE010000000
VERSION     DAZPDE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.191"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHU0J_003487"
     CDS             <1..1076
                     /locus_tag="ACHU0J_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR1218017.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHU0J_003488"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHU0J_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1218018.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHU0J_003489"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHU0J_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1218019.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACHU0J_003490"
     CDS             1897..2343
                     /locus_tag="ACHU0J_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1218020.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHU0J_003491"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHU0J_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACHU0J_003492"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACHU0J_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACHU0J_003493"
     CDS             4450..4797
                     /locus_tag="ACHU0J_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1218021.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHU0J_003494"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHU0J_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1218022.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHU0J_003495"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHU0J_003495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1218023.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACHU0J_003496"
     CDS             6314..6412
                     /locus_tag="ACHU0J_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1218024.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHU0J_003497"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHU0J_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1218025.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1599 a         1599 c         1615 g         1784 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataa
//
LOCUS       DAZPDE010000188         6571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPDE010000188 DAZPDE010000000
VERSION     DAZPDE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.192"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            159..506
                     /locus_tag="ACHU0J_003498"
                     /pseudo
     CDS             159..506
                     /locus_tag="ACHU0J_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            559..954
                     /locus_tag="ACHU0J_003499"
     CDS             559..954
                     /locus_tag="ACHU0J_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1218026.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            995..1738
                     /gene="cmoA"
                     /locus_tag="ACHU0J_003500"
     CDS             995..1738
                     /gene="cmoA"
                     /locus_tag="ACHU0J_003500"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1218027.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1735..2706
                     /gene="cmoB"
                     /locus_tag="ACHU0J_003501"
     CDS             1735..2706
                     /gene="cmoB"
                     /locus_tag="ACHU0J_003501"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1218028.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="ACHU0J_003502"
     CDS             complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="ACHU0J_003502"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1218029.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5325..6425)
                     /locus_tag="ACHU0J_003503"
     CDS             complement(5325..6425)
                     /locus_tag="ACHU0J_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1218030.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1510 a         1718 c         1555 g         1788 t
ORIGIN      
        1 cataatgggc ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca
       61 ctcatagcta accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt
      121 taggaaccat ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg
      181 ttcatgcatc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag
      241 ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg
      301 atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc
      361 agaccactac gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg
      421 tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta
      481 ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc
      541 attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta
      601 atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac
      661 ggtggtttta gcgaactgca aagcgctatt tgcattcatg gtaacgcggt ggaatatatt
      721 cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg
      781 catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac
      841 cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg
      901 gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca
      961 atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc
     1021 tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc
     1081 ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt
     1141 agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc
     1201 ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga
     1261 caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac
     1321 gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt
     1381 ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa
     1441 aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga
     1501 agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg
     1561 ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga
     1621 ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg
     1681 gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc
     1741 gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg
     1801 ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg
     1861 tccaacacgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc
     1921 gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt
     1981 atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat
     2041 accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc
     2101 ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc
     2161 gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa
     2221 gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa
     2281 cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc
     2341 cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg
     2401 gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt
     2461 tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg
     2521 aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag
     2581 cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat
     2641 ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag
     2701 ccgtaaaggt ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
     2761 cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca
     2821 gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga
     2881 acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac
     2941 accagcgcag agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt
     3001 aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca
     3061 tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga
     3121 ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct
     3181 tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca
     3241 tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc
     3301 tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc
     3361 ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc
     3421 agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc
     3481 tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata
     3541 gtgacgcgtt gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc
     3601 atttcatctt taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga
     3661 tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc
     3721 ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat
     3781 gtggtgatcg gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa
     3841 acgacgatca tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg
     3901 gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta
     3961 ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga
     4021 atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac
     4081 atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag
     4141 aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc
     4201 atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt
     4261 gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta
     4321 atttccgccg tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct
     4381 tcaaactgcg gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt
     4441 gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg
     4501 atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca
     4561 atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta
     4621 ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg
     4681 ctgttttcca gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc
     4741 acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa
     4801 ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa
     4861 gagcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc
     4921 tcatgttgct catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct
     4981 tcgccacgac ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga
     5041 tgctgaatac gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt
     5101 atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc
     5161 atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg
     5221 agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat
     5281 tcacgtcttg ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta
     5341 atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg
     5401 ctggttcgtg cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg
     5461 gcggcaatag aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc
     5521 catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga
     5581 cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata
     5641 ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt
     5701 tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca
     5761 acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg
     5821 aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc
     5881 gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg
     5941 atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc
     6001 gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct
     6061 tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca
     6121 ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca
     6181 aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct
     6241 ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg
     6301 caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac
     6361 ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct
     6421 cgcatagatt cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca
     6481 acgaattaac cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat
     6541 gatttattac aatgtaatca ttaattgcta a
//
LOCUS       DAZPDE010000189         6374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPDE010000189 DAZPDE010000000
VERSION     DAZPDE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.193"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHU0J_003504"
     CDS             <1..378
                     /locus_tag="ACHU0J_003504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218031.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHU0J_003505"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHU0J_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1218032.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHU0J_003506"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHU0J_003506"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1218033.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHU0J_003507"
     CDS             complement(1787..2476)
                     /locus_tag="ACHU0J_003507"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1218034.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHU0J_003508"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHU0J_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1218035.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHU0J_003509"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHU0J_003509"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1218036.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHU0J_003510"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHU0J_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1218037.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZPDE010000190         6349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPDE010000190 DAZPDE010000000
VERSION     DAZPDE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.194"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            161..811
                     /gene="csgD"
                     /locus_tag="ACHU0J_003511"
     CDS             161..811
                     /gene="csgD"
                     /locus_tag="ACHU0J_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1218038.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            816..1205
                     /gene="csgE"
                     /locus_tag="ACHU0J_003512"
     CDS             816..1205
                     /gene="csgE"
                     /locus_tag="ACHU0J_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1218039.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1230..1646
                     /gene="csgF"
                     /locus_tag="ACHU0J_003513"
     CDS             1230..1646
                     /gene="csgF"
                     /locus_tag="ACHU0J_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1218040.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1673..2448
                     /locus_tag="ACHU0J_003514"
                     /pseudo
     CDS             1673..2448
                     /locus_tag="ACHU0J_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2512..3003)
                     /locus_tag="ACHU0J_003515"
     CDS             complement(2512..3003)
                     /locus_tag="ACHU0J_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1218041.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3105..3659)
                     /gene="ycdY"
                     /locus_tag="ACHU0J_003516"
     CDS             complement(3105..3659)
                     /gene="ycdY"
                     /locus_tag="ACHU0J_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1218042.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3683..4420)
                     /gene="ycdX"
                     /locus_tag="ACHU0J_003517"
     CDS             complement(3683..4420)
                     /gene="ycdX"
                     /locus_tag="ACHU0J_003517"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1218043.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4475..5413)
                     /gene="ghrA"
                     /locus_tag="ACHU0J_003518"
                     /pseudo
     CDS             complement(4475..5413)
                     /gene="ghrA"
                     /locus_tag="ACHU0J_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5648..5735
                     /locus_tag="ACHU0J_003519"
     tRNA            5648..5735
                     /locus_tag="ACHU0J_003519"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5682..5684,aa:Ser,seq:gga)
     gene            complement(5885..6166)
                     /locus_tag="ACHU0J_003520"
                     /pseudo
     CDS             complement(5885..6166)
                     /locus_tag="ACHU0J_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1706 a         1552 c         1445 g         1646 t
ORIGIN      
        1 gctagattga aatcagatgt aatccattag ttttatattt tacccattta gggctgattt
       61 attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca
      121 gctgtcaggt gtgcgatcaa taaaaaagtg gggtttcatc atgtttaatg aagtccatag
      181 tattcatggt catacattat tgttgatcac taaaccttct ttgcaggcga cagctctctt
      241 gcagcacctt aaacaatcgc tagcaattac aggaaaatta cataatattc aacgttctct
      301 ggacgatatc tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct
      361 tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct
      421 aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt
      481 tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg tcctgcgtgg
      541 cgaatgctac tttacgcaaa agcttgccag ttacctgatt acacattctg gtaactatcg
      601 ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataagct
      661 gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt
      721 taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt
      781 ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg
      841 tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg
      901 gattgttaac tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg
      961 ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat
     1021 ggggaagctg gatcacaata acggtcaatc aggatgttat tttccagact tttttattcc
     1081 cgttgaaaag agactccgag aaaactgtcg tctttgcact gattcaaact gaagaagcac
     1141 taaatcgtcg ccagataaat caggcgttat taagtacgga cgatttggcg catgatgaat
     1201 tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca
     1261 tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact
     1321 ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt
     1381 ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg ttagataact
     1441 ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt aataccggta
     1501 aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat
     1561 tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt
     1621 tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg
     1681 cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga
     1741 agccgccaga ccgacattaa tgcctcgtgc tcagagctac aaagatttga cccatctgcc
     1801 agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt
     1861 taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca gggcttacaa
     1921 aacctgctta acgagcgcaa ggttattcgt gcggcacaag aaaacggcac ggttgccatt
     1981 aataaccgaa tcccgctgca atctttaacg gcggcaaata ttatggttga aggttcgatt
     2041 atcggttatg aaagcaacgt taaatcgggc ggtgttgggg caagatattt tgggatcggt
     2101 gcagacacgc aataccagct cgatcagatt gccgtgaacc tgcgtgtcgt caatgtgagt
     2161 accggtgaga tcctctcctc ggtgaacacc agtaagacga tactttccta tgaagttcag
     2221 gccggggttt tccgctttat tgactaccag cgcttgctgg aaggggaagt gggttacacc
     2281 tcaaacgaac ccgttatgct gtgtctgatg tcggctatcg aaacaggggt cattttcctg
     2341 attaatgatg gtatcgaccg tggtctgtgg gatttgtaaa ataaagcaga acggcagaat
     2401 gacattctgg tgaaataccg ccatatgtcg gttccaccgg aatcctgaca gataaaaatg
     2461 ccggaattaa taatccggca tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt
     2521 atttttgatt tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc
     2581 gctgtttttc attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt
     2641 ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga
     2701 aagcagcagc tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata
     2761 accgagaatt tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac
     2821 cacgccacta agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca
     2881 agcaaagtag gccgtacagc ccaggaagcc cgcccagctt agtaagccca gggaaacagc
     2941 cacccatccc cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt
     3001 catgccgcgc atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc
     3061 aacaacaaat tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct
     3121 tcttccagct cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc
     3181 cagaaaggcg tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac
     3241 agatactcac tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc
     3301 agccaggaag ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtagc
     3361 atccctcgtt cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg
     3421 ctacgatatg gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag
     3481 acttgcgtca tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc
     3541 cagttcgcag ccagtttccc ctcacgaatc agagtaaaca gcggcaccag taaaggatct
     3601 tgcggttggc ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt
     3661 aatatatttc cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc
     3721 gagattcaag gaagttcagt aagcggcacg gagaaacatt caaaatgcgc tctagcggaa
     3781 aatctaccgc gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt
     3841 gagaatccga gcctaatgcc acccaacctc cagcatcacg taccgctgca gctaccgcac
     3901 gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga ggaattattg atttccagcg
     3961 ccacctgatg tttcgcggct gcttcagcaa cggctttaac atctatttca tatttcggat
     4021 ttccgggatg gcttattata tgcacattgc cgctggcgat agtggagatc atcgcttgtg
     4081 tattggtcgc tttgtcatgt ggcgcaaaaa ccggctcatg aaaaccggca ataatcagat
     4141 ccagcgagtc aaacatttta ccgctgcagt caatttcacc atcaacattt ttaatgttag
     4201 cttcaatgcc gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa
     4261 tgaagtgcca gtgatgcggc gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa
     4321 gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg
     4381 tgctggcaac ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat
     4441 tcccgccaag gatagcagga atcctgatgc tttattagta gccacgtgcg cggtcgactt
     4501 gcccgcagac cctctcccct ttttcgagct gggcaatagt gcgagaaatg tactccacag
     4561 cttcagcggg acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc
     4621 agagcggact ttcaggcggt aagggttcac gattaaaaac atccagcatt gcgcctttaa
     4681 ctttcccgct atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca
     4741 ggttgaggag atacgcgcca tcaggtaatt tttcgagtaa ttgttgatta ataatgccga
     4801 cggtttcagg ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag
     4861 acagttcttc ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc
     4921 agcaacgcag cggaaagcgc taggtttgca gactctgagc aactttacag cccagtacgc
     4981 ctgcgcccaa aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat
     5041 gcgaactatt ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac
     5101 tgacagcata ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa
     5161 cagaagggtt cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg
     5221 ccccgagtgc gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc
     5281 agactaaaac ataatcagca gagtcattat ctccgctttt ccatgccctg actcttgcct
     5341 gaggaatagc tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa
     5401 agatgatatc catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt
     5461 atgatctaca aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt
     5521 cgcttattaa aaccacacat tgattgaatg tttgaataaa cgcgcgattt tttcaaaaag
     5581 tttgttgacc tcaggtcatg atttccctaa attagcgccc gttccagcaa gacaggaacg
     5641 acaatttggt gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt gtatacggca
     5701 acgtatcggg ggttcgaatc cccccctcac cgccagatta taagtgcatg ttattgttta
     5761 acatgcgctt ttttattttt ccttccagac agtatgccat cgggtatgcc atctgaaacc
     5821 ggtttatatg ccccgcgatc atatcttatc ggacaatttc cgatctttga gcatactggc
     5881 aaagctatac tgccagcata tcaggatagt gttttgcgat aatatcattc agtctgtcaa
     5941 ccagttctgg ttttctgaac gtgatatgcg cggagccctt cttaaagtat ctgatgctga
     6001 acatctcatc ttcatagctc tctttaccct gaacagactg aatgtgatca tccagacgaa
     6061 gggtgatgtt tccccggttg tcggggattg gtttaccgct gaacaggtgc aacattcttt
     6121 ccagatcggc gagtcggtca gcctgttgtc cggtgataag atgaaatcta tggtcacccc
     6181 cgtttttgca acactgattt ttgattaatg gttggcttgc ttaaatctat ccggcgtctc
     6241 tgtgggcaag gatgcccgcg ccccgatgag ttccgcacct gataatccta aaaatcccct
     6301 cggcttcagt gagccatttc atctgttagg tttttaatca ggtcgattt
//
LOCUS       DAZPDE010000191         6325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPDE010000191 DAZPDE010000000
VERSION     DAZPDE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.195"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHU0J_003521"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHU0J_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1218044.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHU0J_003522"
     CDS             2219..2767
                     /locus_tag="ACHU0J_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1218045.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHU0J_003523"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHU0J_003523"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1218046.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHU0J_003524"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHU0J_003524"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1218047.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHU0J_003525"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHU0J_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1218048.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1596 a         1549 c         1605 g         1575 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaa
//
LOCUS       DAZPDE010000192         6323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPDE010000192 DAZPDE010000000
VERSION     DAZPDE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.196"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..856)
                     /locus_tag="ACHU0J_003526"
     CDS             complement(<1..856)
                     /locus_tag="ACHU0J_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1218049.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNHVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHU0J_003527"
     CDS             964..1242
                     /locus_tag="ACHU0J_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218050.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHU0J_003528"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHU0J_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1218051.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHU0J_003529"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHU0J_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1218052.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHU0J_003530"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHU0J_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1218053.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHU0J_003531"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHU0J_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1218054.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHU0J_003532"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHU0J_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHU0J_003533"
     CDS             complement(4922..5452)
                     /locus_tag="ACHU0J_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1218055.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHU0J_003534"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHU0J_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1218056.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1832 a         1320 c         1370 g         1801 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtggt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcg
//
LOCUS       DAZPDE010000193         6288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPDE010000193 DAZPDE010000000
VERSION     DAZPDE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.197"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHU0J_003535"
     CDS             <1..694
                     /locus_tag="ACHU0J_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1218057.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHU0J_003536"
     CDS             complement(1033..1803)
                     /locus_tag="ACHU0J_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218058.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHU0J_003537"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHU0J_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHU0J_003538"
     CDS             complement(2294..2515)
                     /locus_tag="ACHU0J_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218059.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHU0J_003539"
     CDS             complement(2516..3199)
                     /locus_tag="ACHU0J_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1218060.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHU0J_003540"
     CDS             complement(3263..3574)
                     /locus_tag="ACHU0J_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218061.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHU0J_003541"
     CDS             complement(3571..4473)
                     /locus_tag="ACHU0J_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1218062.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5871
                     /locus_tag="ACHU0J_003542"
                     /pseudo
     CDS             4913..5871
                     /locus_tag="ACHU0J_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            5871..6266
                     /locus_tag="ACHU0J_003543"
     CDS             5871..6266
                     /locus_tag="ACHU0J_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1218063.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
BASE COUNT         1684 a         1670 c         1549 g         1385 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acgaaagcct ccacaccatg taattatttt
     5581 attgctgatg agttaataaa aaacagacac gataatgaat atctccagac attaataaat
     5641 gatgtagctg aaataaaaaa catttctcat gtaatagaca gagaagtgaa atctctggca
     5701 gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta tctcactggc
     5761 ggaggagccg agcttattta ccctcacata aagcaatatt tcccgaatct gaaagtgaac
     5821 aaggtggatg aacctcagtt tgcattggtg aaagcaatgg tgcatgcgta atggaaagca
     5881 gtgatccaaa aaaaagaaag aaagttgtgg catacctgca cccggcactt tatccgcagg
     5941 acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca
     6001 gacaatccct tatttgcgga gcagctcttt attctgttgc cccccgactg ttaacgctaa
     6061 tcagcgtttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa
     6121 caacaggata cacatccaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat
     6181 cttcagaaaa caaatcagag agcatcactt caaaagatga tttcgaagaa caaaccagac
     6241 gcaatctttc tatgttaaaa aaataataaa agaagagcag gttcagtc
//
LOCUS       DAZPDE010000194         6098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPDE010000194 DAZPDE010000000
VERSION     DAZPDE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.198"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..84
                     /locus_tag="ACHU0J_003544"
     CDS             <1..84
                     /locus_tag="ACHU0J_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218064.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHU0J_003545"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHU0J_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACHU0J_003546"
     CDS             complement(1611..2201)
                     /locus_tag="ACHU0J_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1218065.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHU0J_003547"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHU0J_003547"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1218066.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACHU0J_003548"
     CDS             3179..3715
                     /locus_tag="ACHU0J_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1218067.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACHU0J_003549"
     CDS             complement(3756..4616)
                     /locus_tag="ACHU0J_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1218068.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHU0J_003550"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHU0J_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1218069.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHU0J_003551"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHU0J_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1218070.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1563 a         1543 c         1512 g         1480 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa gtgaagaa
//
LOCUS       DAZPDE010000195         6076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPDE010000195 DAZPDE010000000
VERSION     DAZPDE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.199"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHU0J_003552"
     CDS             complement(<1..143)
                     /locus_tag="ACHU0J_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR1218071.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHU0J_003553"
     CDS             379..651
                     /locus_tag="ACHU0J_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR1218072.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHU0J_003554"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHU0J_003554"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1218073.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1749
                     /locus_tag="ACHU0J_003555"
     CDS             1111..1749
                     /locus_tag="ACHU0J_003555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1218074.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHU0J_003556"
                     /pseudo
     CDS             complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHU0J_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3132..3785)
                     /locus_tag="ACHU0J_003557"
     CDS             complement(3132..3785)
                     /locus_tag="ACHU0J_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1218075.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3785..5256)
                     /locus_tag="ACHU0J_003558"
                     /pseudo
     CDS             complement(3785..5256)
                     /locus_tag="ACHU0J_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5229..6076)
                     /locus_tag="ACHU0J_003559"
                     /pseudo
     CDS             complement(5229..6076)
                     /locus_tag="ACHU0J_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1623 c         1607 g         1419 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1681 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1741 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1801 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1861 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1921 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1981 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
     2041 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     2101 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2161 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2221 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2281 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2341 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2401 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2461 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2521 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
     2581 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2641 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     2701 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
     2761 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2821 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2881 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2941 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     3001 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     3061 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     3121 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3181 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3241 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3301 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3361 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3421 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3481 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3541 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3601 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3661 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3721 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3781 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3841 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     3901 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3961 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     4021 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     4081 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4141 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4201 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4261 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4321 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4381 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4441 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4501 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     4561 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     4621 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     4681 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     4741 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     4801 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     4861 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     4921 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     4981 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     5041 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     5101 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     5161 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     5221 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     5281 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     5341 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     5401 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     5461 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     5521 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     5581 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     5641 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     5701 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     5761 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     5821 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     5881 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     5941 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     6001 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     6061 aaattggcct ctttta
//
LOCUS       DAZPDE010000196         6052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPDE010000196 DAZPDE010000000
VERSION     DAZPDE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.200"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            430..1338
                     /locus_tag="ACHU0J_003560"
     CDS             430..1338
                     /locus_tag="ACHU0J_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR1218076.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACHU0J_003561"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACHU0J_003561"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1218077.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="ACHU0J_003562"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="ACHU0J_003562"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1218078.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACHU0J_003563"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACHU0J_003563"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1218079.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="ACHU0J_003564"
     CDS             complement(5673..>6052)
                     /locus_tag="ACHU0J_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218080.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAZPDE010000197         5899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPDE010000197 DAZPDE010000000
VERSION     DAZPDE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.201"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHU0J_003565"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHU0J_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHU0J_003566"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHU0J_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHU0J_003567"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHU0J_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR1218081.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5722..>5899
                     /locus_tag="ACHU0J_003568"
     CDS             5722..>5899
                     /locus_tag="ACHU0J_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218082.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQD"
BASE COUNT         1445 a         1488 c         1409 g         1557 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tagggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttatttagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
     5761 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
     5821 gacccaccac ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc
     5881 cttacccggc tacaggata
//
LOCUS       DAZPDE010000198         5765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPDE010000198 DAZPDE010000000
VERSION     DAZPDE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.202"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..231)
                     /locus_tag="ACHU0J_003569"
     CDS             complement(<1..231)
                     /locus_tag="ACHU0J_003569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218083.1"
                     /translation="MTKNINFSRFALSPYITENSGDLLASIFMPHLPSGVASDSQYVR
                     VEKSDYSHWVIYRHKQIKKSFYCMAIKLKIKNL"
     gene            complement(385..1344)
                     /gene="nrdF"
                     /locus_tag="ACHU0J_003570"
     CDS             complement(385..1344)
                     /gene="nrdF"
                     /locus_tag="ACHU0J_003570"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1218084.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1354..3498)
                     /gene="nrdE"
                     /locus_tag="ACHU0J_003571"
     CDS             complement(1354..3498)
                     /gene="nrdE"
                     /locus_tag="ACHU0J_003571"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1218085.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3471..3881)
                     /gene="nrdI"
                     /locus_tag="ACHU0J_003572"
     CDS             complement(3471..3881)
                     /gene="nrdI"
                     /locus_tag="ACHU0J_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR1218086.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3878..4123)
                     /gene="nrdH"
                     /locus_tag="ACHU0J_003573"
     CDS             complement(3878..4123)
                     /gene="nrdH"
                     /locus_tag="ACHU0J_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1218087.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4371..4700)
                     /locus_tag="ACHU0J_003574"
                     /pseudo
     CDS             complement(4371..4700)
                     /locus_tag="ACHU0J_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            4852..5196
                     /locus_tag="ACHU0J_003575"
     CDS             4852..5196
                     /locus_tag="ACHU0J_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1218088.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5233..5682)
                     /gene="alaE"
                     /locus_tag="ACHU0J_003576"
     CDS             complement(5233..5682)
                     /gene="alaE"
                     /locus_tag="ACHU0J_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1218089.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1475 a         1418 c         1429 g         1443 t
ORIGIN      
        1 aagattttta atttttaatt taatggccat gcaatagaat gatttcttta tttgtttatg
       61 tcgatatatt acccaatggg aatagtcact tttttctacc ctaacatact gagaatctga
      121 ggcaacccct gagggcaaat gtggcatgaa tattgatgcg agcaaatcgc cggaattttc
      181 cgtgatataa gggctgagag caaatcgaga aaagttgata ttttttgtca tagatagcga
      241 aaaaacgcct gaatattgta gtttcaggcg aataatgcaa cagagtatca gtgtagatca
      301 ctacagaata tttgcgtgat aaaaagctat ttggcgggaa ttatttccct gctgcgggta
      361 gtgatatttt tgaaaataac accctcagaa attccagtct tcatcttctg tttcaaccgc
      421 tttccccatc acataagagg agcctgaacc ggaaaagaaa tcgtgatttt catcggcatt
      481 cggcgaaagc gcggcgagga ttgccggatt cacttccgcc atttctgcgg gaaataacgg
      541 ttcgtagccc agattcatca aagccttatt ggtgttgtaa cagagaaacg ctttcacatc
      601 gtcagcccac ggggtttcgg cgtacagctc atcggtgtat tgcaactcgt tgtcgtagag
      661 ttccagcaac aaatcgaagg cgaaactctt caactcttca cgttgtccca gagatatctt
      721 ttccatgttt ttctgatatt tatagcctat gtagtaaccg tggactgctt catcgcggat
      781 aatcagacgg atcaggtccg cggtattggt cagctttccg cggctggaaa aatacatcgg
      841 cagccagaaa ccggaataga acaaaaaaga ttcaagaaac acactggcga ttttcttttt
      901 cagcggatca tcaccgcgat aatgttgctg aataatctga gcttttcgct gcaacggtgc
      961 gttttcttca ctccaggcgt aggcggcatc gacatctttg gtctggcaca gcgtcgagaa
     1021 aatcgaactg taagagcggg catgaaccgc ttccataaag ctgatattcg ataataccgc
     1081 ctcttcatga ggcgtgagcg catcgggcat cagcgaaggc gcgccgataa cattttgcag
     1141 cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc agttgttgtt ctacgacagt
     1201 taatgtctgc caggcgggaa tatcgttcga cagcggcacc ttttctggta gccagaaatt
     1261 gctggtcagg cgattccaca cctccagatc tttatcgtca gatatcttgt tccagttgat
     1321 ggcgctgata cgtgagagtt tcatagatat tccttaaagt gcacaggaga cgcagccttc
     1381 aatttcagtg ccttccagcg ccatctgacg caggcggatg taatagagcg ttttgatacc
     1441 tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga gtggtggcgg tatcggggaa
     1501 aaacagcgtc agcgacagcc cctgatcgac atggcgagtc gcttccgcgt aggtgtcgat
     1561 gatcttttct gcgccaattt cgtaagcgtc ctgatacagc gccagatttt cgttagtcat
     1621 aaacggggca gggtagtaaa cgcgtcctgt tttgccctct ttgcgtatct ctactttcgc
     1681 cacaatcgga tgaatactcg acgtagcatg gttgatataa gagatagacc cggttggcgg
     1741 caccgcctgg agattctggt tgtatatgcc gtagcgcatc acgtcgtcgc gcagttgcgc
     1801 ccacatctca cgggtaggta acgtaataca gctgcgggca aacagttcgc caactttcgc
     1861 cgttttcggc tgccagttac cttgcagata ttggctgaaa tattcaccac tggcatagcg
     1921 tgactgtttg aacccggcga aggtttcacc gcgttcgcgc gccagcaaca tcgaggtacg
     1981 cagtgcgtgc caggtgatgg tatagaaata gagattggtg aaatccaggg cttccggcga
     2041 accataagcg atgccttccc tcgccagata accatgtaaa ttcatctgcc ccagtccgat
     2101 ggcgtgcgag gcggcatttc cggcttcgat ggacggcacg ctgcggatat gactcatatc
     2161 tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg cgggaaaaat cgggggaatc
     2221 catggtgtgc gcaatattca acgaacctaa attgcaggaa atatcatggc ctgtgcgggc
     2281 atagtcgaga ttctcgtcat actctgaggc gctgttaacc tgcaaaattt ctgagcagag
     2341 attactcata tttatgcgcc cggcgatagg gttagcacgg tttactgtgt cttcatacat
     2401 gatgtaggga tagccggact caaactggat ttctgccagt cgctggaaga aatcacgggc
     2461 gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt tcgtcatagt gttcgctaat
     2521 ggcgatatcg gcaaacggct tgccataaac tcgctcaacg tcatagggcg aaaacagcgc
     2581 catctgcgca ttctcttttg ccagatggaa agtgatatcc gggatcacca cgcccagcga
     2641 cagtgtttta atgcggattt tttcgtcggc attttcccgt ttcgtgtcga gaaaacgcag
     2701 aatatcggga tgatgagcat gtaaatagac tgcacctgcc ccctgacgcg cgccgagttg
     2761 gttggcatag gaaaatgcgt cttccaacat tttcatcacc ggaattacgc cagaagattg
     2821 attttcaata cgtttaattg gcgcgcccgc ttcccgcaga ttcgacagca aaaatgctac
     2881 gccgccgccg cgtttcgaca gctgcagtgc ggaatttacc gcccgaccaa tcgactccat
     2941 attgtcttca atacgcagca aaaaacagga aaccagttcg ccgcgctgct gcttaccgca
     3001 gttgaggaat gttggcgtgg ctggctgaaa gcgtcctgac agcatttcat cggtcagttg
     3061 caacgccagc gtctcatcgc cttgtgccag cgtcagcgcc accatcgtga ctcgatcggc
     3121 aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac gtatagctgg tgtaaaactt
     3181 ccatgccccg aggaatgtct ggaaacgaaa accgctggtg tgtgcgtggg caaacagcgt
     3241 aatgacaaag tcgcgagagt agcgattaag aacgctttca tcatagtaac cttcgttgac
     3301 cagccagttc aggcgctgct gctggctact gaaggtcaca ctgttcggac gcacatgcgt
     3361 cgcaataaag gcgtcaacgg cctggcggtc tttatcgaac tgaatgcgac ctgcgctatc
     3421 gtaaagatta agcatcgcat tcagcgcgtg gtaatccatc gtttcctgcg tcaggcattc
     3481 tgcggttgtc gttgccaaaa ttcggttact cctttacgaa cgttttcgat atcgctttgc
     3541 gtacccatga gttcaaaacg gtacagccac ggcacgccgc atttccgggc aatcacatct
     3601 ccggcgcggc catacgcctc accaaagttg cgattaccag aagcaataac gccgcgaagc
     3661 aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct gtcgtggcac cgcgccagcc
     3721 gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag gctcgtctac ctgaatccgt
     3781 tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta aacgttcgat aaaacgctgc
     3841 gtgttttcgg agctgctgga gaagtagacg agctggctca tgcactggcc gcgtgtggcg
     3901 ctggatgcag acggttaatc atgtccggac ggaaaccaga ccagctaaga tcgccagcaa
     3961 tcactaccgg caactgacga aagccctgag cacgcaacgc ttccgctgct tcaggaacgc
     4021 gatcgacatt aatcatttca aaatcaaagc cccggttttc catcgcccgt ttggtggcgt
     4081 ggcactgaac gcaatcgtta cgagtataaa tagttatgcg catgattcgt atttccgttt
     4141 aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct ctctgttgat acagagatac
     4201 tagatgtagt tgaaaaaaga ttcaactaca caatatatag caaatagtga tcgaaattac
     4261 cctggatatg agcgtgacgg gggaggggga tttttgtgat tcaccaggca aaaagaaacc
     4321 ccgaagacag gcttcggggt caaagacgcg tatttattat catttttgca ctacgatttg
     4381 cgcatgctta acagtgcgcc gataaaaata cctaccgcag ctgctgtacc cacgctacac
     4441 cagggtcttt cacgaacaaa agaatccgct cagccaacgg catcgcgcgc ggcttgctgg
     4501 acgcgagtac gaccatgcat tcgcgccctg gtttctttca gcaacgcctg agctttactg
     4561 cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg acttcaatac agattcgagg
     4621 ctgtcagcta attgattgac atcattctgg atatcctgca cgccatcatc aacgtcgttg
     4681 cggttcggtc ggttaaacat atgatccccc attagaattc gatgtaattt tcagtgtaga
     4741 ccataatttt cttaactgag gttcatcgcg gctttatgga cttttctgaa agcgttgtga
     4801 atgcgcaatc aggtaaggta ggcccgcggt tgaaaataac gaggatcaaa tatgtattta
     4861 cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga tgtggtaacc
     4921 cagaaagcgt atggttaccg ccgtggggaa aattatgtct atgttaatcg cgaagcgcga
     4981 atgggacgta ccgctctggt aattcatcca acattaaaag aacgcagttc aacactggct
     5041 gaacccgctt ccgatattaa aacttgcgat cattaccagc aatttccgct ctatttagca
     5101 ggcgagcgac acgagcatta cggtatcccg catggcttta gttcgcgtgt tgcgcttgaa
     5161 cgttatttga atggtttatt tggcgaagcc agttaaagac gcgactggcg atgccagtcg
     5221 cgaaaagaag agtcaggctt ttacctgctg gtaacggctg actttaaaca gtcggcggca
     5281 ataatcgagg aagtagccat aaaccgcccc catcaacatc gaaacaacga tgtttgaact
     5341 gaccgccgcc ataatctgat gccagtctgc gcccaccact aacaagatcg ccacatacac
     5401 cggtgactgg aacgtcacat aagccaggat atcagccaga ttttttatcc agcccgacgg
     5461 gctaactttg cgtgccgcgc gcataaacag atcgcggtac ataccgtatg gccatgcaat
     5521 taagatgttc accggaatcg ctaccaatct ggaataaaaa gactgttcga agctcattcc
     5581 ggagaggaaa acttcaatac acatgttcac gacagaacag taaacaacca tcgcgaacgt
     5641 atctgcaact gcatgacgca agcgtgactg cggtgagaac atgctaatgc tccttaataa
     5701 aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc tcattgccat ctgcattttt
     5761 gcata
//
LOCUS       DAZPDE010000199         5744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPDE010000199 DAZPDE010000000
VERSION     DAZPDE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.203"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..173
                     /locus_tag="ACHU0J_003577"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACHU0J_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACHU0J_003578"
     CDS             complement(489..827)
                     /locus_tag="ACHU0J_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218090.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACHU0J_003579"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACHU0J_003579"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1218091.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACHU0J_003580"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACHU0J_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACHU0J_003581"
     CDS             complement(2165..2650)
                     /locus_tag="ACHU0J_003581"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1218092.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACHU0J_003582"
     CDS             complement(2906..3886)
                     /locus_tag="ACHU0J_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR1218093.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACHU0J_003583"
     CDS             complement(3886..5085)
                     /locus_tag="ACHU0J_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1218094.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACHU0J_003584"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACHU0J_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACHU0J_003585"
     CDS             complement(5407..>5744)
                     /locus_tag="ACHU0J_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1218095.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZPDE010000200         5740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPDE010000200 DAZPDE010000000
VERSION     DAZPDE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.204"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHU0J_003586"
     CDS             <1..241
                     /locus_tag="ACHU0J_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218096.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHU0J_003587"
     CDS             complement(238..1179)
                     /locus_tag="ACHU0J_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR1218097.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHU0J_003588"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHU0J_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHU0J_003589"
     CDS             complement(1684..2904)
                     /locus_tag="ACHU0J_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1218098.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHU0J_003590"
     CDS             complement(2908..3651)
                     /locus_tag="ACHU0J_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1218099.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHU0J_003591"
     CDS             complement(3542..5008)
                     /locus_tag="ACHU0J_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR1218100.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5740)
                     /locus_tag="ACHU0J_003592"
                     /pseudo
     CDS             complement(5008..5740)
                     /locus_tag="ACHU0J_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1436 g         1460 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgattg
//
LOCUS       DAZPDE010000201         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPDE010000201 DAZPDE010000000
VERSION     DAZPDE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.205"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHU0J_003593"
     CDS             complement(<1..170)
                     /locus_tag="ACHU0J_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218101.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHU0J_003594"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHU0J_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1218102.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHU0J_003595"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHU0J_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHU0J_003596"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHU0J_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1218103.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHU0J_003597"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHU0J_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHU0J_003598"
     CDS             5484..>5702
                     /locus_tag="ACHU0J_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR1218104.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZPDE010000202         5681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPDE010000202 DAZPDE010000000
VERSION     DAZPDE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.206"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHU0J_003599"
     CDS             <1..171
                     /locus_tag="ACHU0J_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218105.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHU0J_003600"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHU0J_003600"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1218106.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHU0J_003601"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHU0J_003601"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1218107.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHU0J_003602"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHU0J_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1218108.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHU0J_003603"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHU0J_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1218109.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..>5681
                     /locus_tag="ACHU0J_003604"
     CDS             5430..>5681
                     /locus_tag="ACHU0J_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218110.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDT"
BASE COUNT         1423 a         1244 c         1465 g         1549 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac g
//
LOCUS       DAZPDE010000203         5655 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPDE010000203 DAZPDE010000000
VERSION     DAZPDE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.207"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHU0J_003605"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHU0J_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1218111.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHU0J_003606"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHU0J_003606"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1218112.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHU0J_003607"
     CDS             complement(3736..4092)
                     /locus_tag="ACHU0J_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1218113.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHU0J_003608"
     CDS             complement(4096..4512)
                     /locus_tag="ACHU0J_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1218114.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHU0J_003609"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHU0J_003609"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1218115.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1348 a         1475 c         1466 g         1366 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaa
//
LOCUS       DAZPDE010000204         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPDE010000204 DAZPDE010000000
VERSION     DAZPDE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.208"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHU0J_003610"
     CDS             complement(1..279)
                     /locus_tag="ACHU0J_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218116.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHU0J_003611"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHU0J_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1218117.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHU0J_003612"
     CDS             complement(1254..1670)
                     /locus_tag="ACHU0J_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218118.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHU0J_003613"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHU0J_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHU0J_003614"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHU0J_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHU0J_003615"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHU0J_003615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHU0J_003616"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHU0J_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1218119.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHU0J_003617"
     tRNA            3996..4071
                     /locus_tag="ACHU0J_003617"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHU0J_003618"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHU0J_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZPDE010000205         5557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPDE010000205 DAZPDE010000000
VERSION     DAZPDE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.209"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..641
                     /locus_tag="ACHU0J_003619"
     CDS             <1..641
                     /locus_tag="ACHU0J_003619"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR1218120.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHU0J_003620"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHU0J_003620"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1218121.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHU0J_003621"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHU0J_003621"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1218122.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZPDE010000206         5547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPDE010000206 DAZPDE010000000
VERSION     DAZPDE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.210"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            221..1153
                     /gene="rfaD"
                     /locus_tag="ACHU0J_003622"
     CDS             221..1153
                     /gene="rfaD"
                     /locus_tag="ACHU0J_003622"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1218123.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1163..2209
                     /gene="rfaF"
                     /locus_tag="ACHU0J_003623"
     CDS             1163..2209
                     /gene="rfaF"
                     /locus_tag="ACHU0J_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1218124.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2213..3205
                     /gene="rfaC"
                     /locus_tag="ACHU0J_003624"
     CDS             2213..3205
                     /gene="rfaC"
                     /locus_tag="ACHU0J_003624"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1218125.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3202..4410
                     /gene="rfaL"
                     /locus_tag="ACHU0J_003625"
     CDS             3202..4410
                     /gene="rfaL"
                     /locus_tag="ACHU0J_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR1218126.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4446..5546)
                     /locus_tag="ACHU0J_003626"
     CDS             complement(4446..5546)
                     /locus_tag="ACHU0J_003626"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR1218127.1"
                     /translation="MPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFVNDNC
                     SIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYTQIR
                     KLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLET
                     YQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGD
                     YTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPLADLVVIPSQFQEPF
                     CMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELT
                     AVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1522 a         1220 c         1259 g         1546 t
ORIGIN      
        1 ttccgttatt tatcatacat cggaatattg atactaaagc actattcaca tgcaaaacca
       61 acatccgcca tgaaggacta gctaaaaccc aaactagttt gttgcaatta gcatccttgc
      121 acctctatgt aaagggctga agggattcgg atgtgatggt atgattacag acattcgtgt
      181 ctgagattgt ctctgactcc ataattcgaa ggttacagtt atgatcatcg ttaccggcgg
      241 cgcgggcttt atcggcagca acatcgttaa ggccctgaat gataaaggca tcaccgatat
      301 tctggtggtg gacaacctga aagacggcac caagtttgtg aacctggtgg atctggatat
      361 cgcggactat atggataagg aagacttcct gatccagatt atggctggcg aagagttcgg
      421 tgatgtcgaa gcgattttcc acgaaggcgc atgctcttcc accaccgagt gggatggcaa
      481 gtatatgatg gataacaact atcaatactc caaagagctg ctgcactact gtctggagcg
      541 cgaaatcccg ttcctgtatg cctcttccgc agccacctac ggcggacgca cctccgactt
      601 tattgaatcc cgcgagtacg aaaaaccgtt gaatgtctac ggttactcaa aattcctgtt
      661 tgatgaatat gttcgtcaaa tcctgccaga agcgaactcg cagattgttg gcttccgcta
      721 tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc atggcgagcg tcgctttcca
      781 tctcaacacc cagcttaata acggtgaatc accgaagctg ttcgaaggta gcgagaactt
      841 caaacgtgac ttcgtctatg tgggcgacgt ggcagatgta aacctgtggt tcctggaaaa
      901 tggcgtttcc ggtatcttca acctcggcac cggtcgtgca gaatccttcc aggcagtagc
      961 agatgctacg ctggcttatc acaagaaagg ccagatcgaa tacattccgt tcccggataa
     1021 gctgaaaggc cgctaccagg cgttcactca ggcagatctg acaaatctgc gcgcggcggg
     1081 ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg cctggctgaa
     1141 tcgcgacgca taagagctct gcatgaaaat actggtgatc ggcccgtctt gggttggcga
     1201 catgatgatg tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc aggcgataat
     1261 cgatgtgatg gcaccggcat ggtgccgtcc attattatcg cggatgccgg aagttaacga
     1321 agctatccct atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc gcaaactggg
     1381 tcatagcctg cgtgaaaagc gctacgaccg cgcctacgtc ttaccaaact ccttcaaatc
     1441 tgcattagtg cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg gcgagatgcg
     1501 ctacggttta ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc taatggtgga
     1561 acgctatgtc gcgctggcct atgacaaagg cattatgcgt accgcacaag atctgccgca
     1621 gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata cctgtaatca
     1681 attttcgctt tcatcagaac gtccgatgat tggcttttgc ccgggtgcgg agtttggtcc
     1741 ggcaaaacgc tggccacact accactatgc ggaactggca aagcagctga ttgatgaagg
     1801 ttatcaggtg gttctgtttg gctctgcgaa agatcatgaa gcgggcaatg agattcttgc
     1861 cgctttgaat accgagcagc aggcatggtg tcggaacctg gcgggggaaa cacagcttga
     1921 tcaagcggtt atcttgattg cagcctgtaa agccattgtc actaacgatt ctggcctgat
     1981 gcacgttgcg gcggcgctca atcgtccgct ggttgccctg tatggtccaa gtagcccgga
     2041 cttcacaccg ccgctatccc ataaagcgcg cgtgatccgt ctgattaccg gctatcacaa
     2101 agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc ttgatcgaca ttactcccca
     2161 gcgcgtactg gaagaactca acgcgctatt gttacaagag gaagcctgac ggatgcgggt
     2221 tttgatcgtt aaaacatcgt cgatgggcga tgttctccat acgttgcccg cactcactga
     2281 tgcccagcag gcaatcccag ggattaagtt tgactgggtg gtggaagaag ggttcgcaca
     2341 gattccttcc tggcacgctg ccgttgagcg agttattcct gtggcaatac gtcgctggcg
     2401 taaagcctgg ttctcggccc ccataaaagc tgaacgcaaa gcgtttcgtg aagcgctaca
     2461 agcagagaac tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg cggcactggt
     2521 gacacgtctg gcgcatggcg taaagcatgg attggactgg caaaccgctc gcgaaccttt
     2581 agccagcctg ttttacaatt gtaagcatca tattgcaaaa cagcagcacg ccgtagaacg
     2641 cacccgcgaa ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc agggcgatta
     2701 tgctatcgca cagcattttc tgacgaacct gcctacagat gctggcgaat atgccgtatt
     2761 tcttcatgcg acgacccgtg atgataaaca ctggccggaa gaacactggc gagaattgat
     2821 tggtttactg gctgattcag gaatacggat taaacttccg tggggcgcgc cgcatgagga
     2881 agaacgggcg aaacgactgg cggaaggatt tgcttatgtt gaagtattgc cgaagatgag
     2941 tctggaaggc gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg tggatacggg
     3001 gttaagccat ttaacggcgg cactggatag acccaatatc acggtttatg gaccaaccga
     3061 tccgggatta attggtgggt atgggaagaa tcaagtagag tgtcgttcta caagtatgtc
     3121 tcttgcagat ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa taataaccaa
     3181 taagttgaca tcggagataa gatgacctca acattatttt tctctctcga gaaaaaaaac
     3241 tggatagcgt actggaacag agctctcgta ttcttattca ttaccaccta ttttttgggt
     3301 gggattacaa ggtataaaca tcttattgtt attcttatga caataacgac aatcgtctat
     3361 ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa catttctttt tggtagtgtt
     3421 gccatattaa ctattgctgc attgctgtca cttcttcaat cccctgatgc aggtgctagc
     3481 atgaaggaag ttttcaaagc tattattgag aatactttac tatgcacaat agcaataccg
     3541 gtcatattga gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt ctcatttatt
     3601 agtgcgttgg gcttacgctg tttttctgaa ttgattacct attataagga ctatcaacaa
     3661 gggataatgc cattcgcaga ttatagacac cgtagcattt ctgactcgat ggtcttttta
     3721 ttccctgcat tgttaaatct ctggcttatc aaatcagcaa aataccgcat ttcttttgtg
     3781 gttctaagcg ttatttttat ttttctgata ttaggaactt tatccagagg ggcctggctt
     3841 tccgtgttag tcattggatt aatatggatt ctgatgttta aacaatggaa gttactatta
     3901 gtaggagtaa tggtttccat cattgcattg tcggttattt tcacacataa ggagatgacc
     3961 gcaaagctaa cgtataaact tcaacaaact aatagttctt atcgctatgc aaatggtact
     4021 caaggcagcg cactcgatct aatattagaa aatcctgtta ttggttatgg ttacggtaac
     4081 gttgcatata aagatgtcta taataaacgt gtcattgatt atccagaatg gacctttaga
     4141 caatcaatag ggccacataa ttttgcgcta ttcatctggt ttggcactgg tttattaggg
     4201 ctggtaagtc ttatgatgct atactgtgca atattgaaag agtgtataaa aaatggcgtc
     4261 aagaataaat atcgctcacc atataatgca tattatataa tcttactatc ttttataggt
     4321 tattttgtta tccgtggaaa cgtagaacaa attgaaccaa atttattagg cgtttacgcc
     4381 ggcttattat tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata atgcggcctt
     4441 tttatttatt caaaccaatt atgaataacc tcttcgaatc tttgagttac gccatcccaa
     4501 ctgtagtgat caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa ttcaggattc
     4561 gctaacgttt ttaaaatatc gctgcttatg gagtctgcag tcataggttc ttttaaatgg
     4621 aatcccgtag tattttcttt gacaaattcc gtcatccccc cacgcgtact cactaaaaca
     4681 ggcttaccag cacccatagc ttcaattgcc accatgcaaa atggttcctg gaactgggaa
     4741 ggaatgacga ctaaatctgc cagtgggtaa taacaataca tcttttcagg cggtatgctt
     4801 cctaacataa tgcaacgatc cttcaaacgt tttgcaattt ccctgacatt gcgttgatat
     4861 gcacctttgt cacttttact catttctgta taatcaccaa tcacaactaa ttttaaattt
     4921 ttgtgagcag cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc tttgtcaggg
     4981 acgatacgcc cagcataaaa aataattttc tcttcaggag agatattaag ttcgcttcta
     5041 gtgatcggct gaaaattaga ttgataggtt tctaaatcaa ttccatttgg tacaatctca
     5101 atatctgcat ttgctaaata agattggtaa tactttttca agtataaact agggactatc
     5161 attttaacat tttgctcaag caattttggt tcaaatgcat tatgcatatg tataaccact
     5221 cttgcctgag gagctagctt acgaatttga gtgtatagct tcatgctatt atgaactaca
     5281 ataacacttt ctttcgttat aggaaaatca tgtgcaatat tcaatattct ctgtgaatat
     5341 ggaagaggat caagtcttgt ccatttttga aataaccgct tatatatgcg actgaatcca
     5401 accctatgaa tactacaatt atcattaaca aaggtgtaat ttgagtatcc tggatttttg
     5461 atacaaacaa tacgatttgg aaaattggta cgctgagcaa cctgatacat ccacgtttca
     5521 acagcagccg ctccgcgagg aggaatt
//
LOCUS       DAZPDE010000207         5495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPDE010000207 DAZPDE010000000
VERSION     DAZPDE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.211"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..213
                     /locus_tag="ACHU0J_003627"
                     /pseudo
     CDS             1..213
                     /locus_tag="ACHU0J_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(710..1086)
                     /gene="rnpB"
                     /locus_tag="ACHU0J_003628"
     ncRNA           complement(710..1086)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHU0J_003628"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1119..2264)
                     /gene="garK"
                     /locus_tag="ACHU0J_003629"
     CDS             complement(1119..2264)
                     /gene="garK"
                     /locus_tag="ACHU0J_003629"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1218128.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2361..3251)
                     /gene="garR"
                     /locus_tag="ACHU0J_003630"
     CDS             complement(2361..3251)
                     /gene="garR"
                     /locus_tag="ACHU0J_003630"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1218129.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3281..4051)
                     /gene="garL"
                     /locus_tag="ACHU0J_003631"
                     /pseudo
     CDS             complement(3281..4051)
                     /gene="garL"
                     /locus_tag="ACHU0J_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(4067..5401)
                     /gene="garP"
                     /locus_tag="ACHU0J_003632"
     CDS             complement(4067..5401)
                     /gene="garP"
                     /locus_tag="ACHU0J_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1218130.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1360 a         1535 c         1292 g         1308 t
ORIGIN      
        1 ttatctggtc gtcccccaaa ttaattatac tatggattta aacatagaag actctagctc
       61 agaagagtta ataccctcga gaaagacgaa aattctccgg acaatggctt tgaggtcggg
      121 gaatataaca catatgaagc atataactca gagaatcaat attttaccag agaggactat
      181 acgtataatt acgacctttt aaatgcaata tagacttaat aacttcttac aaagtctatt
      241 attaataccg tagatattca tcttaccctg tgcaccaaaa gcaaaaactt atcgacgcgg
      301 gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct acgcttatca ggcctacgcc
      361 atctctgcaa tatattgaat ttgcgtgctt ttgtaggcag gataaggcgt tcacgcagca
      421 tccggcatga ataaagcgca ctcaacaatc tgaaacccgc cggaacggtt tattacgtac
      481 atcaggtgaa actgaccgat aagccgcttt cttttgggta tagtgtcgtg gacagtcatt
      541 catctttctg cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg tatatcaggt
      601 gaaactgacc gataagccgc tttcttttgg gtatagcgtc gtggacagtc attcatcttt
      661 ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt acgtaaaaca ggtgaaactg
      721 accgataagc cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg
      781 ctcaagcagc ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg
      841 ctccgggtgg agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc
      901 accctttcac ccttacctga tcccgcttgc acgggccatc ggcggtttgc tctctgttgc
      961 actggtcgtg ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact
     1021 ttcctcccct ccacccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc
     1081 agcttcggcg cgcagtatag agggtttgcg cgcccttgtc accccgtgtt gcgcattcca
     1141 atcgccagtg tcgcggcaat attacgtgaa gcacggcaga tattgtcata agccccgcgg
     1201 aatgcttcgt ccaacgtacc tatgctggtc aatacgctga agaccgcatc aatgccatgc
     1261 tgatgtacaa cgccaacatc atcggtcagg ctacccgcaa tgccaatcac cggtttatgg
     1321 tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc cgtgaatact ctggctgtca
     1381 atacgccctt caccggtgat caccagcgta caatcgtgaa tatgttcctc cagattcagc
     1441 gccgtagtga cgatttcaat accacttttc agttccgcac caagaaacgc catcagcgcc
     1501 gcacccatac cgcccgcagc tcctgcgccg gggacatctt ttacatcaac atgcagcgct
     1561 tttttaatga catcggcata gtgagagagg ttattgtcca gctcaacaat catcgcttca
     1621 ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt tatcgcccac cagcggattg
     1681 gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc gcggatcgag gccggaaata
     1741 tcaatatcat tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggcg
     1801 tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc cgccatcatt tgtagcgcta
     1861 ccgccaatgc cgataataat gtttgtcgcg ccgctctcca gcgcctgcag gattaactcg
     1921 cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac cagctccagc
     1981 ccactggccg ccgccatttc aataaacgcg gttttgccat cgccggagat cccccaactg
     2041 gcattcactt tctcgcccag cggccctgta acccaggcgt gacgttcaga accctgggtg
     2101 gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg caaccggaat agaaacgtac
     2161 tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca
     2221 gataaacttt ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc
     2281 acatgacgcc ggagggcgtt tctcttattc ggcctggatt ccaggcccgg attgcaatac
     2341 gccatccggg cacgacgtca ttaacgagta acttcgactt tcgccagttt ttcgtagtag
     2401 cacaccaggg cgctatgatc cgccgttcct aaaccatctg ctcgcagtgc ctgcatcatc
     2461 tccataaccg cagctgtgag cggcagttgc gcgccgacgc cgtgggaagt atccagcgca
     2521 tttgccagat ccttaatatg cagatcaata cggaagcccg gcttgaagtt gcggtccatc
     2581 accatcggcg ctttggcatc cagcacggta ctgcccgcca gtccaccgcg aattgcctga
     2641 tacaccaggt ccgggttaac gcccgcttta gttgccagcg ttaacgcttc tgacatcgcg
     2701 gcaatattca gcgccacaat gacctgattc gccagtttgg tgacgttacc tgcaccaatt
     2761 gccccggtat gcaccacgga acccgccatt gctttcatca aatcatagta tttgtcgaaa
     2821 atagccttgt cgccgcccac catcactgac agcgtaccgt cgatggcttt cggttcaccg
     2881 ccgctcaccg gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca
     2941 cggcttgcca gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct
     3001 tcaataatgc cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg
     3061 gttatgatga cgtcgcactg ttcagcgatc gctttagccg tagaggctgt ttctgcacct
     3121 gcagcaatca cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct
     3181 gctttcagaa ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact
     3241 ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag
     3301 ccagtttatg agtggcagag cggaagacgc cgagatcgct gccgacagcc acaaacgtcg
     3361 cgccccattc cagataacga cgcgcatcgg cttcgaccgg cgcgaggata ccgctgggtt
     3421 tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca tccgggtgtg
     3481 atgcattgcc gagatggcct aatgccgcgg ccagatcgct ggggccgacg aagatgccgt
     3541 ctacgccttc ggtagcggca atggcatcga cgttatctac gccctgctga ctttctatct
     3601 ggaccagaat agtgatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca
     3661 tattggcgcg gtgagaaacg gagacgccgc gaatgccttc cggtgggtaa cgggttgatg
     3721 ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac
     3781 cgatatccag aagacgctta ataatttccg gctcgttggt cggcactcac actactggcg
     3841 cgctggcgct gcctttcaag gccattaact gcggaataaa cgtggagata tcgtttggcg
     3901 catgttcgcc atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa
     3961 tcgggttaga gagtgctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt
     4021 tgaatttatt cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt
     4081 ccatacgttt aatgtcgcca actacgaaga ggtagcagac catcgccatc agcgctgaac
     4141 atcccacgaa aaccagtgct gcattgaagg agtgcagttc acttaccagg tagccaatca
     4201 ccagtggagt gacaatggag gcaacattgc caaagacgtt aaagacgccg ccacagaggc
     4261 caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt
     4321 tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac
     4381 ataagatgat ggtggaagcc agcaacattc ccagcacaat cggtagctta cgtgccaggg
     4441 tcagggataa accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg
     4501 caaaaccaca cagtgccgga atcgaggcga ccagacccac tttcagaatc gacatgcctt
     4561 tttcctgcac cagataaatc gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa
     4621 aatattgtcc gaaaaatacg cccaacatca tgcggttaga gagcaattgc ttgatgtaat
     4681 gcagtttggg accgcttgct gccgcgctgc ccggcttttt gtggtccata tcgaccaccg
     4741 cgccattttc agagataaac ttcagctctt cctcagacat acgtgggtga tctgtcgggt
     4801 tatgaatcaa cttgatccac agcgccgtca gcacaaaaca aatcaccccc ataacggtaa
     4861 agacgtgctc ccatccccag gcgaaagtca gccagccaag cagaggcgaa aagagcgcca
     4921 gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga
     4981 accaggcggc gacaatacgg gcgttcgccg ggaaggatgg agcttccgag aatcccagca
     5041 taaagcgcat aaagaacatg gagatccctg cccaagccag cgggaacata tcaacaaagc
     5101 cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta ggtgtaaact tttttcgagc
     5161 caaacttatc aagcaaccag ccgccgggga tttgcatcag caagtaggcc cagccaaaag
     5221 cggagaagat gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac
     5281 cagcaataga cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca
     5341 gtaatattaa atagcgggta tgcacacctt tccttttttc gtcaacggtg tccagaatca
     5401 ttttatttac ctcgggtact tatgctgatt tttattatta tggggaaggt gttatttata
     5461 agtttcattt atgccgtaac gacaatgaac tcggg
//
LOCUS       DAZPDE010000208         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPDE010000208 DAZPDE010000000
VERSION     DAZPDE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.212"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHU0J_003633"
     CDS             complement(<1..1630)
                     /locus_tag="ACHU0J_003633"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1218131.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHU0J_003634"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHU0J_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHU0J_003635"
     CDS             complement(2541..3146)
                     /locus_tag="ACHU0J_003635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1218132.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHU0J_003636"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHU0J_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR1218133.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZPDE010000209         5484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPDE010000209 DAZPDE010000000
VERSION     DAZPDE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.213"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(174..809)
                     /gene="msrQ"
                     /locus_tag="ACHU0J_003637"
     CDS             complement(174..809)
                     /gene="msrQ"
                     /locus_tag="ACHU0J_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1218134.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(810..1814)
                     /gene="msrP"
                     /locus_tag="ACHU0J_003638"
                     /pseudo
     CDS             complement(810..1814)
                     /gene="msrP"
                     /locus_tag="ACHU0J_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            complement(1923..2336)
                     /gene="hiuH"
                     /locus_tag="ACHU0J_003639"
     CDS             complement(1923..2336)
                     /gene="hiuH"
                     /locus_tag="ACHU0J_003639"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1218135.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2469..3140
                     /gene="hprR"
                     /locus_tag="ACHU0J_003640"
     CDS             2469..3140
                     /gene="hprR"
                     /locus_tag="ACHU0J_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1218136.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3140..4498
                     /gene="hprS"
                     /locus_tag="ACHU0J_003641"
     CDS             3140..4498
                     /gene="hprS"
                     /locus_tag="ACHU0J_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1218137.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4606..5457)
                     /gene="hchA"
                     /locus_tag="ACHU0J_003642"
     CDS             complement(4606..5457)
                     /gene="hchA"
                     /locus_tag="ACHU0J_003642"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1218138.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT         1521 a         1177 c         1300 g         1486 t
ORIGIN      
        1 agagcatggt attctcaggt gtattgaact atcttattga aataaaataa attattctgg
       61 tgatgaaagg ttgatgacaa acgaaacata acttgatgcg atctgtgtgg gccggatagg
      121 gcgctcgcgc cgcatccggc cgggataacg cgaagtttgt cggcaatatg aagttataga
      181 gataatttgt tgtgggcctg cttgcgtaac cggttgaaaa gggaaagcaa cttcttatac
      241 cgtaaggcta aaagcagtac agccagcccg gcgtagatga gtggctgcgg tgaaataatc
      301 ttcactgacc acagataatg tattggggcc aggatcgcga caagatagac gaagttatgc
      361 aactgttgcc agtgtttgcc gagttttcgc tgcatcgact gggttgaagt gaacgctaaa
      421 gcaagcaaaa taacccagct gataattcct aacgttaaat aaggccgggt aattaactcc
      481 ttacctaata acgccagatt attcacgccc aactccagca atgcgtaact ggttaaatgc
      541 agtgtcgccc aggcaaagca ccataagcct aacaggcggc gagtgcgtat caataacggc
      601 tgttttgcgt agcgtgccag aggtgtgatt aacaaggccg caagcaaaaa tttcagtgca
      661 gtgcgaccgg taaaatgctg aatatctttc accggatcgg cacccagtcc accgtgattg
      721 atcgcccaga ccagccagag aaacggcaac aatccggcaa gatgcaggca aactttcagc
      781 catatcactt gttttgctgt cagacgcatt tagaaattct cccgcaaatc caggccacga
      841 tacagcgatg ccacctggtc ggcgtaacca ttaaacagta gtgttggctg gcgctgaaca
      901 tcgagaatgc cgcctgaacc aataaatcgt tcggtagcct gtgaccagcg cgggtgatca
      961 acatgcggat taacgttggc gtaaaaaccg tattcgtcag gcgctgccag attccaggtg
     1021 gttggcggac gctcgcgggt cagcttaata ctgacgatcg atttaatccc tttaaagcca
     1081 tatttccacg gcacaatcag tcgcaccggc gcgccatttt gcggtggtaa cgccttgcca
     1141 taaacaccta cggtcatcag tgtgagcgga tgcattgctt cgtcgagacg caatccttcg
     1201 acataaggat atttcagccc gccgccgata aagcggtcct gctggccagg catctgttcc
     1261 ggcgcataaa tcgtttcgaa agcgacatac ttcgcattgc tggtgggttc ggcaagcgcc
     1321 agcaatttgt gtagcggaaa accaatccac ggcaccacca tcgaccacgc ttccacgcag
     1381 cgcatacgat aaatacgctc ttccagcggg aagcgacggg ttaaatcatc atgatcgagg
     1441 gtcaatggtt ttgccacttc gccgctgatt ttcagtgtcc atggatcggt tttcaggcta
     1501 ccagcattag ccgcgggatc gtctttatcc agcccgaatt catagaagtt gttataaccg
     1561 gagactttat ctgctggcgt cagtggcagg ttattttgct aggcggcagg cttgctgaac
     1621 tccagcggtt ttccggcggg ggcgggcgga cgatcgttcc ctttaaacca gctaagcaga
     1681 tcggcatgcg cagcgtgagg caaagaaaat gcagctgcgc tgatgcccag tgcttttaac
     1741 acctgccgac gcttcataaa gaataccgac tcggccgtaa catctgattc ttttaaaaat
     1801 tgcttctttt tcataagcca tcacactttt ttgtcgttaa ccaacaagag ctgtttactg
     1861 ttaatctgtc agggagtcac ctccctaaca gtagcagaga tcagctttta catatttacg
     1921 cattaactgc cacgataggt tgaataccca tattggctga gtaacaaagg cacatgataa
     1981 tgctcgttca ctttattaat atgaaattca acgggaatct cagggaagaa actttcaaga
     2041 ttttgtttct tgaaatagtc cccggtttta aatacgacac ggtaatcgcc agtagtcgca
     2101 gtttgctcgg gccacagtgc cttaattcgt ccatccttat ctgttttggc ggtattaagt
     2161 tgtaaccagc cgttatccgc tttcttttca agagtgactg tcacgtcggc agcgggtttt
     2221 cctgtttgct ggttcaaaat gtgcacgcta agaatgtttt gttgtgcggc ataaaccaaa
     2281 gaaggtaatg aaaaggctgc cgttgctacc gagagtacta aataacgctt taacatgttt
     2341 atatccttgt catgtgaatg agttttgagg atagcgaaaa aacgtccagg gtaaccttac
     2401 agcagcatta caattttgta atgaaccagg ttgtttctat aacatatgat ttatggcata
     2461 ttattttcat gaagattcta cttattgaag ataatcaaag gacccaggaa tgggtaacgc
     2521 aggggctttc cgaagcgggt tatgtcattg atgccgtttc tgatggcaga gatgggcttt
     2581 atcttgcgct gaaggatgat tatgcattga tcattctgga tattatgctt ccgggtatgg
     2641 atggctggca gatcttacaa acgttaagaa cggcaaagca aacccctgtt atttgcctta
     2701 ctgcaaggga ttctgtcgat gacagagtca gagggttgga cagtggggca aatgattatc
     2761 tggtaaaacc tttttcattt tctgagttgc tagcaagggt tcgggcacaa ttaaggcaac
     2821 atcacgctct gaattcaaca ttagaaatca gtggcttaaa aatggactct gtcagtcaaa
     2881 gtgtcagcag ggacaatatc agtattacac tgacgcgcaa ggagtttcag ttactttggc
     2941 tactggcctc cagagctggc gagattatac ccagaacggt tattgcgagt gaaatttggg
     3001 gaatcaactt tgatagtgat accaatacgg tggatgtcgc cattcgcagg ctccgcgcaa
     3061 aagttgatga tccttttcct gaaaagctaa tcgccacaat ccgggggatg ggctattcat
     3121 tcgtagcggt aaaaaaataa tgaaaagatt atctataacc gtccgtttaa ccttgctttt
     3181 tatatttctg cagtctgttg ctggcgctgg aattgtatgg actctctata atggcctggc
     3241 aagtgagttg aaatggcgcg atgatacaac actcattaac cggacagcgc agatcaagca
     3301 gttgttaatt gatggggtaa atccagatac gttacctgtg tactttaacc ggatgatgga
     3361 tgttagtcag gatattttga tcattcatag tgatggcatc aataaaattg ttaaccggac
     3421 aaatgtcagt gatgacatgt taaataacat acctgctagt gagacaatca gcgcagcagg
     3481 catttacaga agcattatta atgatacaga gatagatgct ttacgaatta acattgatga
     3541 agtttcgcca tcattaacgg ttactgtggc taaattggct tcagccagac ataatatgct
     3601 tgaacagtat aaaattaata gcattataat ttgcattgtc gccattatac tgtgctcagt
     3661 attaagtccg ttgttaatca ggacggggtt gcgagagatc aaaaagttga gtggcgtaac
     3721 ggaagcgctg aattataacg atagccgagt gccggttgag gttaacgcat taccgagaga
     3781 actaaaacct cttgggcagg cgttgaataa aatgcatcat gctttagtca aagattttga
     3841 gcgccttagt caatttgctg acgatctcgc tcatgaactt agaacgccaa ttaatgcatt
     3901 actgggtcag aatcaggtta cgctcagtca aatcagaagt atcgctgaat atcaaaaaac
     3961 aattgccgga aacattgaag agctggaaaa tatttcgcgg ttaacagaga atatactgtt
     4021 tcttgccagg gcagacaaaa acaatgtttt ggtgaaactg gattcgcttt ctctcaataa
     4081 ggaagttgaa aatcttttgg actatcttga atatctttca gacgagaaag agatttgctt
     4141 taaggtcaag tgcaatcaac aaatctttgc ggataaaatt ttactgcaac gaatgttatc
     4201 gaatcttatt gttaacgcca ttcgatattc accagaaaaa tcgcgtattc atataaccag
     4261 ttttcttgat gccaacggct ctcttaatat tgatatcgcc agtcctggaa cgaaaattaa
     4321 tgagcctgaa aaactcttcc gtagattttg gcggggagat aattcgcgtc attccgtagg
     4381 tcagggactt ggcctttctt tagtcaaagc gattgccgaa ttacatggag gaagtgctac
     4441 gtatcactat ctcagtaagc ataacgtgtt ccgaattacg ttaccgcaaa gaaattaaca
     4501 tcgggcttca gtacgccata agttaaccgt gaaaaaacgc agccacgtcg tgagtattaa
     4561 cgtggctgcg attgattatg cgcttacatc acaatgtaac agggattaac tcgcgtaagc
     4621 tgccagcatt tcctgcgccg ccagtttacc caacgcattc gctgcaaaag gactgtcgcc
     4681 ggtgagacgt ttacggtcct tatgtactcg cccggtgatg tcgtcattaa tgatgttcat
     4741 gcccattttc ttcaactctt cgccgaagta ccaggtgaga tgacccggca tatagccaat
     4801 atctggcgtt tgtttgtctg cggcgtctgg gaatgcgcaa atagaataac cattcagtgg
     4861 gttatcgctg tggcgaagtg ccagaaaagc cgccggaccg tggcaaaggg agataacaaa
     4921 acggtcattt ttgattgccc actgtaaagc ggcagccacg tcctggcttt ctggtaaacc
     4981 aataagtgcg ccatgcccac caggaacaaa gattgctgca tattcgctat cagcgttgag
     5041 gctggcgaca acatccgcga gtttcttcgg attacggaat aacgatttat gctgttcaaa
     5101 gaatggcatt actttttcat ctttgtgcgg catagcccag tattcaaact tggtcatcag
     5161 accggaaatg gtcgccactt cgaattcgaa acctgcagca tggagatgat acaacggcag
     5221 caacgtttca atcggatggt taccggtcga gaacagtttt ccgttatcgg tcggcaaata
     5281 acgttcgtcc gcggcgatca ccagaatttt atgtttaccg cgatacggtt ttggatagtc
     5341 cacgccatca agatcagaga cgggactcgt atattggcta agcgaatatt ctgaagggaa
     5401 gaacgcatta tcttcagcaa tatcgacctg cgggttttta cttgtttgaa cagtcatagt
     5461 gtattcctta ttgttgctta gtta
//
LOCUS       DAZPDE010000210         5455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPDE010000210 DAZPDE010000000
VERSION     DAZPDE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.214"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            499..1488
                     /gene="araF"
                     /locus_tag="ACHU0J_003643"
                     /pseudo
     CDS             499..1488
                     /gene="araF"
                     /locus_tag="ACHU0J_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1558..3072
                     /gene="araG"
                     /locus_tag="ACHU0J_003644"
     CDS             1558..3072
                     /gene="araG"
                     /locus_tag="ACHU0J_003644"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1218139.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3087..4073
                     /gene="araH"
                     /locus_tag="ACHU0J_003645"
     CDS             3087..4073
                     /gene="araH"
                     /locus_tag="ACHU0J_003645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1218140.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4240..5040
                     /gene="otsB"
                     /locus_tag="ACHU0J_003646"
     CDS             4240..5040
                     /gene="otsB"
                     /locus_tag="ACHU0J_003646"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1218141.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5015..5455
                     /locus_tag="ACHU0J_003647"
                     /pseudo
     CDS             5015..5455
                     /locus_tag="ACHU0J_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1344 a         1267 c         1461 g         1383 t
ORIGIN      
        1 ttttataaaa cgttttttca tttttgcgag atggctctca ttatacgtgt tctgttaatt
       61 aatgagcaca gtgataatta atggcgaaat gaatgacgcg cattttccac atctttgagt
      121 tgtggttatt acaccatttc aaaaactcaa cgccaggtaa tgcggcctat tgactggtta
      181 aaaagaagac atcccgcatg ggtaccgaag acaacaagga tttccaggct aatcttatgg
      241 attaatctgc tgtgcattca acaatttgtc tgacaaattg gcttttcctt atgtcttttc
      301 ccgctaaatt tatgcacgtt ctcactgtaa ttctgcgatg tgatattgct ctcctatgga
      361 gaattaattt ctcgctaaaa ctatgtcaac acggtcactt atcttttaga taaaaggtaa
      421 tgctttgttt tccgattaat ttaacgaatg tcattcgttt ttgccctaca caaaacgaca
      481 ctaaagctgg agagaaccat gcacaaattt actaaagccc tggcagccat tggtctggca
      541 gccgttatgt cacaatccgc tatggcggag aacctgaagc tcggttttct ggtgaagcaa
      601 ccggaagagc cgtggttcca gaccgaatgg aagtttgccg ataaagccgg gaaggattta
      661 gggtttgagg ttattaagat tgccgtgccg gatggcgaaa aaacattgaa cgcgatcgac
      721 agcctggcag ccagtggcgc aaaaggtttc gttatttgta ctccggaccc gaaactcggc
      781 tctgccatcg tcgcgaaagc gcgtggctac gatatgaaag tcattgcagt ggatgaccag
      841 tttgttaacg ccaaaggtaa gccaatggat accgttcctc tggtgatgat ggcggcgact
      901 aaaattggcg aacgtcaggg ctaggaactg tataaagaga tgcagaaacg tggctgggat
      961 gtcaaagaaa gcgcagtgat ggcgattacc gccaacgaac tggataccgc ccgccgccgt
     1021 acaacgggtt ctatggacgc actgaaagct gcaggattcc cggaaaaaca aatttatcag
     1081 gtacctacca aatctaacga catcccgggg gcatttgacg ctgccaactc aatgctggtt
     1141 caacatccgg aagttaaaca ttggctgatc gtcggtatga acgacagcac cgtacttggc
     1201 ggcgtacgtg caaccgaagg tcagggcttt aaagcagccg atatcatcgg tattggtatt
     1261 aacggtgtgg atgcagtaag cgaactgtct aaagcacagg caaccggctt ctacggttcc
     1321 ctgctgccaa gcccggacgt acatggctat aaatccagcg aaatgcttta caactgggta
     1381 gcaaaagacg ttgaaacgcc aaaatttacc gaagttacag acgtggtact gatcacgcgt
     1441 gacaacttta aagaagaact ggagaaaaaa ggtttaggcg gtaagtaatt tgccggaaaa
     1501 attcccctct gcatgatgca gagggggtgt gaacgaccag tgattcacgg agacgttatg
     1561 caacagtcta ccccgtatct ctcatttcgc ggcatcggta aaacgtttcc cggcgttaag
     1621 gcgctgacgg atattagttt tgactgctat gccggtcagg ttcatgcgtt gatgggtgaa
     1681 aatggcgcag gaaaatcaac tctcttaaaa atcctcagcg gcaactatgc gccaaccacg
     1741 ggttctgtag tgattaatgg gcaggaaatg tccttttccg acacgaccgc agcacttaac
     1801 gcgggtgtgg cgattattta ccaggaactg catctcgtgc cggaaatgac cgtcgcggaa
     1861 aacatctatc tcggccagct gccgcataaa ggcggcattg tgaatcgctc attgctgaat
     1921 tatgaggcgg gtttacaact taaacatctt ggtatggata ttgacccgga cacgccgctg
     1981 aaatatctcg ccattggtca gtggcagatg gttgaaatcg ccaaagcgct ggcgcgtaac
     2041 gctaaaatta tcgcctttga tgagccaacc agctcccttt ctgcccgcga aatcggcaat
     2101 cttttccgcg ttattcgtga actgcgaaaa gaggggcggg tgatcttata cgtttctcac
     2161 cgtatggaag aaatatttgc cctcagcgat gccatcactg tctttaaaga tggacgttat
     2221 gtcaaaacct ttaccgatat gcagcaggtt gaccacgacg cgctggtgca ggcgatggtc
     2281 gggcgcgaca ttggcgatat ctacggctgg caacctcgta gttatggcga ggagcgcctg
     2341 cgtcttgatg ctgtgaaagc actaggcgtg cgtacgccaa taagtctggc ggttcgcagt
     2401 ggtgaaattg ttgggctgtt tggtctggta ggggcggggc gtagcgaatt aatgaaaggc
     2461 ttgtttggcg ggacgcaaat caccgccggt caggtttata tcgaccaaca gccgatcgat
     2521 attcgtaaac cgagccacgc cattgccgca ggcatgatgc tctgcccgga agctcgcaaa
     2581 gcggaaggca ttattcccgt gcactccgtt cgcgacaata tcaacatcag tgccagacgc
     2641 aaacatgtgc tcggcggttg tgtaatcaac aacagttggg aagaaaacaa tgccgatcag
     2701 cacattcgtt cgctcaacat caaaacgccg ggcgcggagc aactgatcat gaatctctca
     2761 ggcggaaatc agcaaaaagc cattctgggc cgctggttat cggaagagat gaaggtcatt
     2821 ttgctggatg aacctacgcg cggcattgat gttggcgcta agcacgaaat atataacgta
     2881 atttatgcgc tggcggcgca gggcgtggcg gtgctgtttg cctccagcga cttacctgaa
     2941 gtcctcggcg ttgccgaccg gattgtggtg atgcgggaag gcgaaatcgc cggtgaattg
     3001 ttacacgagc aggcagatga gcgtcaggca ctgagccttg cgatgcctaa agtcagccag
     3061 gcagttgcct gagtaaggag agtatgatgt cttctgtttc tacatcgggg tctggcgcac
     3121 ctaagtcgtc attcagcttc gggcgtatct gggatcagta cggcatgctg gtggtgtttg
     3181 cggtgctctt tatcgcctgt gccatttttg tcccaaattt tgccaccttc attaatatga
     3241 aagggttggg cctggcaatt tccatgtcgg ggatggtggc gtgtggcatg ttgttctgcc
     3301 ttgcttccgg tgactttgac ctttctgtcg cctccgtaat tgcctgtgcg ggtgtcacca
     3361 cggcggtggt tatcaacctg actgaaagcc tgtggattgg cgtggcagcg ggtttgttgc
     3421 tgggcgttct ctgtggcctg gtcaatggct ttgttatcgc caaactgaaa ataaatgctc
     3481 tgatcacgac actggcaacg atgcagattg ttcgaggtct ggcgtacatc atttcagacg
     3541 gtaaagcggt cggtatcgaa gatgaaagct tctttgccct tggttacgct aactggttcg
     3601 gtctgcctgc gccaatctgg ctcaccgtcg cgtgtctgat tatctttggt ttgctgctga
     3661 ataaaaccac ctttggtcgt aataccctgg cgattggcgg gaacgaagag gctgcgcgtc
     3721 tggcgggtgt accggttgtt cgcaccaaaa ttattatctt tgttctctct ggcctggtat
     3781 ctgcgatagc cggaattatt ctggcttcac gtatgactag tgggcagcca atgacgtcga
     3841 ttggttatga gcttattgtt atctccgcct gcgttttagg tggcgtttct ctgaaaggtg
     3901 gcatcggaaa aatctcatat gtggtggcgg gtatcttaat tttaggcacc gtggaaaacg
     3961 ccatgaacct gcttaatatt tctcctttcg cgcagtacgt ggttcgcggc ttaatcctgc
     4021 tggcagcggt gatcttcgac cgttacaagc aaaaagcgaa gcgcactgtc tgatgctttt
     4081 ttccacaaca acttaacgtt ttttcccacc acagccagcc gccacaacgg ttggctgttc
     4141 ttcgttgcaa atggcgacct ccgtcacact gtctatactt acatgtctgt aaagcgcggt
     4201 ctgcgcaaca caataagaaa agagaaggag gagagccggg tgacagaacc gttaaccgaa
     4261 acccctgaac tatccgcgaa atatgcctgg ttttttgatc ttgatggaac gctggcggaa
     4321 atcaaaccgc atcccgatca ggtcgtcgtg cctgacaata ttctgcaagg actacagcta
     4381 ctggcaaccg caagtgatgg tgcattggca ttgatatcag ggcgctcaat ggtggagctt
     4441 gacgcactgg caaaacctta tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt
     4501 gacatcaatg gtaaaacaca tatcgttcat ctgccggatg cgattgcgcg tgatattagc
     4561 gtgcaactgc atacggtcat cgctcagtat cccggcgcgg agctggaggc gaaagggatg
     4621 gcttttgcgc tgcattatcg tcaggctccg cagcatgaag acgcattaat gacattagcg
     4681 caacgtatta ctcagatctg gccacaaatg gcgttacagc agggaaagtg tgttgtcgag
     4741 atcaaaccga gaggtaccag taaaggtgaa gcgattgcag cctttatgca ggaagctcct
     4801 tttatcgggc gaacgcccgt atttctgggc gatgatttaa ccgatgaatc tggcttcgca
     4861 gtcgttaacc gactgggcgg aatgtcagta aaaattggca caggtgcaac tcaggcatca
     4921 tggcgactgg cgggtgtgcc ggatgtctgg agctggcttg aaatgataac cgccgcatta
     4981 caacaaaaaa gagaaaataa caggagtgat gactatgagt cgtttagtcg tagtatctaa
     5041 ccggattgca ccaccagacg agcacgccgc cagtgccggt ggccttgccg ttggcatact
     5101 gggggcactg aaagccgcag gcggactgtg gtttggctgg agtggtgaaa cagggaatga
     5161 ggatcagccg ctaaaaaagg taacattacg tgggcctctt ttaacctcag cgaacaggac
     5221 cttgacgaat actacaacca attctccaat gccgttctct ggcccgcttt tcattatcgg
     5281 ctcgatctgg tgcaatttca gcgtcctgcc tgggacggct atctacgcgt aaatgcgttg
     5341 ctggcagata aattactgcc gctgttgcaa gacgatgaca ttatctggat ccacgattat
     5401 cacctgttgc cctttgcgca tgaattacgc aaacgtgggg tgaataatcg cattg
//
LOCUS       DAZPDE010000211         5398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPDE010000211 DAZPDE010000000
VERSION     DAZPDE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.215"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHU0J_003648"
     CDS             <1..497
                     /locus_tag="ACHU0J_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR1218142.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHU0J_003649"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHU0J_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHU0J_003650"
     CDS             2500..3114
                     /locus_tag="ACHU0J_003650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1218143.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHU0J_003651"
     CDS             3114..3443
                     /locus_tag="ACHU0J_003651"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1218144.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHU0J_003652"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHU0J_003652"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1218145.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHU0J_003653"
     CDS             4529..4840
                     /locus_tag="ACHU0J_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1218146.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHU0J_003654"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHU0J_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZPDE010000212         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPDE010000212 DAZPDE010000000
VERSION     DAZPDE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.216"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHU0J_003655"
     CDS             complement(99..581)
                     /locus_tag="ACHU0J_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218147.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHU0J_003656"
     CDS             complement(649..2598)
                     /locus_tag="ACHU0J_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1218148.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHU0J_003657"
     CDS             complement(2683..3018)
                     /locus_tag="ACHU0J_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1218149.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHU0J_003658"
     CDS             complement(3018..3374)
                     /locus_tag="ACHU0J_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1218150.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHU0J_003659"
     CDS             complement(3374..4870)
                     /locus_tag="ACHU0J_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1218151.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHU0J_003660"
     CDS             complement(4867..5028)
                     /locus_tag="ACHU0J_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR1218152.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHU0J_003661"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHU0J_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218153.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZPDE010000213         5355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPDE010000213 DAZPDE010000000
VERSION     DAZPDE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.217"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            59..2095
                     /gene="yihQ"
                     /locus_tag="ACHU0J_003662"
                     /pseudo
     CDS             59..2095
                     /gene="yihQ"
                     /locus_tag="ACHU0J_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            2141..3515
                     /locus_tag="ACHU0J_003663"
                     /pseudo
     CDS             2141..3515
                     /locus_tag="ACHU0J_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3558..4961
                     /locus_tag="ACHU0J_003664"
     CDS             3558..4961
                     /locus_tag="ACHU0J_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1218154.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            5029..>5355
                     /locus_tag="ACHU0J_003665"
     CDS             5029..>5355
                     /locus_tag="ACHU0J_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR1218155.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         1263 a         1321 c         1348 g         1423 t
ORIGIN      
        1 ataagcctgt aataacagga aggcaacaat ctcgttctca actctatgga gattagttat
       61 ggatacgcca cgtccacagt tattagattt tcaatttcat cagaataacg acagttttac
      121 cctacatttt caacaacgtc ttattttaac ccataacaaa gataatcctt gtttatggat
      181 tggctcaggt atagcggata tcgatatgtt ccgcggtaat ttcagcatta aagataaact
      241 acaggagaaa attgcgctta ccgacgccat cgtcagccag tcaccggatg gttggttaat
      301 tcatttcagc cgtggttctg acattagcgc cacgctgaat atctctgccg acgatcaggg
      361 ccgtttattg ctggaactac aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct
      421 tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa cagttttcct acttcgatct
      481 gcgcggcaaa ccgttcccgc tatggaccag tgaacaaggc gttggtcgca acaaacaaac
      541 ctatgtcacc tagcaggccg actgcaaaga aaatgcgggc ggcgactatt actggacttt
      601 cttcccacag cctacgtttg tcagcacgca gaagtattac tgccatgttg ataacagttg
      661 ctacatgaac ttcgacttta gcgccccgga ataccatgaa ctggcgctgt gggaagacaa
      721 agctacgcta cgttttgaat gtgctgacac atacatctcc ctgctggaaa aattaactgc
      781 cctgctggga cgccagccag aactgcccga ctggatttat gacggggtaa cgctcggcat
      841 tcagggcggg acggaagtgt gccagaagaa actggacacc atgcgtaacg cgggcgtgaa
      901 ggttaacggc atctgggcgc aggactggtc tgggatccgt ataacctctt ttggcaagcg
      961 cgtgatgtgg aactggaagt ggaacagcga aaactacccg caactggatt cacgcattaa
     1021 gcagtggtat caggagggcg tgcagttcct ggcctatatc aacccgtatg ttgccagcga
     1081 taaagatctc tgcgaagaag cggcacaaca cggctatcta gcaaaagatg cctctggcgg
     1141 tgactatctg gtggagtttg gcgagtttta cggcggcgtt gtcgatctca ctaatccaga
     1201 agcctacgcc tggttcaagg aagtgatcaa aaagaacatg attgaactcg gctgcggcgg
     1261 ctggatggct gacttcggcg agtatctgcc caccgacacg tacctgcata acggcgtcag
     1321 tgccgaaatt atgcataacg cctggcctgc gctgtgggcg aagtgtaact acgaagccct
     1381 tgaagaaacg ggcaagctcg gcgagatcct cttctttatg cgcgccggtt ctaccggtag
     1441 ccagaaatac tccaccatga tgtgggcggg cgaccagaac gtggactgga gtctcgacga
     1501 tggcctggcg tccgtagtcc cggcggcgct gtcgctggca atgaccggac atggtctgca
     1561 tcacagcgac attggcggtt acaccaccct gtttgagatg aagcgcagca aagagctgct
     1621 gctgcgctgg tgcgatttca gcgccttcac gccgatgatg cgcacccacg aaggtaaccg
     1681 tcctggcgac aactggcagt ttgacggcga tgcggaaact atcgcccatt ttgcccgtat
     1741 gactaccgtt ttcaccaccc tgaaacctta tctgaaagag gccgtcgcgc tgaatgcgaa
     1801 gtccggtctg ccggtcatgc gcccgctgtt cctgcattac gaagacgatg cacataccta
     1861 ttccctgaaa tatcagtacc tgttaggccg cgacattctg gtcgctccgg tacacgaaga
     1921 aggtcgcagc gactggacgc tctatctgcc ggaggataac tgggtacatg cctggacggg
     1981 tgaagcgttc cggggcgggg aagttaccgt taatgcgccc atcggcaagc cgccggtctt
     2041 ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg ttaaacagca tctaatcaca
     2101 tccgcccgtg gggaaactca tgggcaaagg gagaacatcg atgagtcaca tcacaacgga
     2161 agatccagca actttacgcc tgccctttaa agagaaactc tcttacggta ttggcgacct
     2221 ggcctctaac atcctgctgg atatcggtac gctttatctt ttgaagtttt ataccgacgt
     2281 tctggggctg ccaggcacct atggcggcat tatctttttg atttcaaaat tctttactgc
     2341 gtttaccgat atgggtaccg gcattatgtt ggattcccga cgcaagatcg gtccaaaagg
     2401 taagttccgt ccttttattc tgtatgcgtc attcccggtc accttactgg cgatcgccaa
     2461 ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg atggccacta ttctgtttat
     2521 gctttacgga ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg tcccggcaat
     2581 caccaaaaac cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct
     2641 gggcctgctg ctgtgcacgg tgggattcgt gccagttatg aatcttatcg aaggtaatca
     2701 gaaacttggc tatatcttcg ccgccacgct gttttcactg ttcggcctgc tgtttatgtg
     2761 gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca atccggcgca
     2821 aaagcctggc ctgttgcaat ctttccgcgc aattgccggt aaccgcccac tgttcattct
     2881 gtgtattgcc aacctctgca ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta
     2941 ttacacccag tacgtactta acgatcccat cctgttgtcg tatatgggat ttttcagcat
     3001 gggctgtatt ttcatcggcg tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa
     3061 gaaggtctat atcggcggcc tgctgatttg ggtgctgggc gatctgctca actatttctt
     3121 cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgcc
     3181 tgaactgggc gctggtttcc gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt
     3241 cggaaggcac ggtctacacc ggcttcacct tctttcgcaa agtgtctcag gcgctggctg
     3301 gtttcttccc cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg
     3361 accacaccat tgaagggtta cgccagttga tcttcatcta cccaagcgca ctggcggtag
     3421 tcaccattgt ggcgatgggt tgcttctaca gcctgaacga gaagatgtat gtccgcattg
     3481 tggaagagat agaagcccgt aaacgcacgg cgtaataata ataaacgacg ccctgcgggg
     3541 cgttataagg agtgattatg tctgaccata atccactgac attaaaactg aatctgcggg
     3601 aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc atcggtactc
     3661 tgtatctcct caaactttat accgatgaat tagggatgcc tgcttactat ggcgggatta
     3721 tctttctggt cgcgaagttt tttaccgcgt ttaccgatat gctcaccgga tttttactcg
     3781 actcgcggaa aaatattggg cctaaaggca aatttcgtcc ctttatttta tatgcagcag
     3841 ttccggcggc gttaattgca acgctccagt ttatcgccac caccttttgt ttgccggtta
     3901 aaacgacgat tgccaccacg ctgtttatga tgtttggact ttcatacagt ctgatgaact
     3961 gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg
     4021 cggcttaccg ccagggtggt gcaaccatag ggctattgat ttgtaccgtg gcgtttattc
     4081 cgttgcagtc gcttttttct gactcaaccg tcggttatgc ctgtgcggca cttatgttct
     4141 ccattggcgg ctttattttt atgatgctgt gctacagagg cgtcaaagag cattatgtgg
     4201 acacagcgcc aaccggacat aaagccagta ttctcaaatc tttttgcgcg atatttcgta
     4261 atccaccatt gctggtttta tgcattgcta acctgtgtac tctggcggca tttaatatca
     4321 aactggcgat tcaggtctat tacacccagt atgtgctgaa tgatattaat ttattgtcgt
     4381 ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt attactggtg ccagtaactg
     4441 taaaatgttt tggtaaaaaa caggtttatt tagctggcat ggtgctatgg gcggtgggtg
     4501 atatactgaa ttatttctgg ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg
     4561 ccttttttgg cacggccttt gtaaacagcc tgaactgggc actggtacca gataccgtag
     4621 attacggtga atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc ggttatacct
     4681 tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa
     4741 ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt acagggtttg cgtcagctca
     4801 tttttatctg gccttgtgca ctggcaatta ttgctgcatt gacgatggga ttcttttaca
     4861 cactcaatga aaaacggttt gcattgatta ttgaggaaat caaccaacgc aaaaataaag
     4921 agatggcgac agaaaaaaaa acggcttccg taacgttata aatgtcaata gccgctattt
     4981 ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg gacttattat gaaaaagatt
     5041 aatgcaataa ttttattatc ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc
     5101 gaaaacaggg aggcatataa tcttgcctcc gatcagggcg aggttatgct acgtgtgggt
     5161 tataacttcg atatgggcgc gggtattatg ttaaccaata cctacacctt tcagcgagaa
     5221 gatgaactaa aacatggcta taatgaaatt gaaggttggt atcctttatt caaaccaacc
     5281 gataaattaa ccatccagcc cggtggctta attaatgata agagtatcag ttcgggtggt
     5341 gcagtgtatc tggac
//
LOCUS       DAZPDE010000214         5339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPDE010000214 DAZPDE010000000
VERSION     DAZPDE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.218"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHU0J_003666"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHU0J_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1218156.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHU0J_003667"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHU0J_003667"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1218157.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHU0J_003668"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHU0J_003668"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1218158.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHU0J_003669"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHU0J_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1218159.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZPDE010000215         5163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPDE010000215 DAZPDE010000000
VERSION     DAZPDE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.219"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHU0J_003670"
     CDS             <1..220
                     /locus_tag="ACHU0J_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218160.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHU0J_003671"
     CDS             complement(392..721)
                     /locus_tag="ACHU0J_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1218161.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHU0J_003672"
     CDS             complement(726..911)
                     /locus_tag="ACHU0J_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1218162.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHU0J_003673"
     CDS             complement(908..3547)
                     /locus_tag="ACHU0J_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1218163.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHU0J_003674"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHU0J_003674"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1218164.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHU0J_003675"
     CDS             4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHU0J_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1218165.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1307 a         1323 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aac
//
LOCUS       DAZPDE010000216         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPDE010000216 DAZPDE010000000
VERSION     DAZPDE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.220"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHU0J_003676"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHU0J_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHU0J_003677"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHU0J_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1218166.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHU0J_003678"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHU0J_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1218167.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHU0J_003679"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHU0J_003679"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1218168.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHU0J_003680"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHU0J_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHU0J_003681"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHU0J_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHU0J_003682"
     CDS             complement(3604..4419)
                     /locus_tag="ACHU0J_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1218169.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHU0J_003683"
     CDS             4516..>5150
                     /locus_tag="ACHU0J_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1218170.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1225 g         1376 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZPDE010000217         5145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPDE010000217 DAZPDE010000000
VERSION     DAZPDE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.221"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            513..1898
                     /gene="dcuC"
                     /locus_tag="ACHU0J_003684"
     CDS             513..1898
                     /gene="dcuC"
                     /locus_tag="ACHU0J_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1218171.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1939..2280)
                     /locus_tag="ACHU0J_003685"
                     /pseudo
     CDS             complement(1939..2280)
                     /locus_tag="ACHU0J_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2618..3127)
                     /locus_tag="ACHU0J_003686"
     CDS             complement(2618..3127)
                     /locus_tag="ACHU0J_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1218172.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            3299..3535
                     /locus_tag="ACHU0J_003687"
     CDS             3299..3535
                     /locus_tag="ACHU0J_003687"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218173.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            3510..5141
                     /locus_tag="ACHU0J_003688"
                     /pseudo
     CDS             3510..5141
                     /locus_tag="ACHU0J_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1309 a         1126 c         1257 g         1453 t
ORIGIN      
        1 aacataaaga ataatctgaa tagctgctct atttatttcc gcttgttcaa gttaaatatt
       61 tccgcgcatc gattaaagat gatgcgtcgt tttctgcgat gggaatagtc aaaaaagaaa
      121 aaccaagtct tttttgatga caaaatgcaa tcaaggaaaa attattttat ttttttaacg
      181 agatacaaca atcatcttaa cgaagtatat aatatatatc gtcaacccat gctcatatta
      241 gattattttt tgagcgcaca gccgcagcta acaccattgc aattaacaaa tttgcatcaa
      301 tccaccatca atttacacac attattatgt gataattgcc aaccgctaaa tatgcgtttt
      361 gttatctatg tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa
      421 tcagcattca tcgccaattt attgggcata ttttttcctt aagctttagg aagtttttat
      481 tatttacttt gggcctggag acaggaaaaa ttatgctgac attcattgaa ctccttattg
      541 gggttgtggt tattgtgggt gtggctcgct acatcattaa agggtattcc gccactggtg
      601 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      661 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      721 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggctttg
      781 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      841 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtt gcttgtctga
      901 tgtcactggc cgtctcttcc gcaaccggtc tgggggtttt gctgatggca accctgtttc
      961 cggtgatggt aaacgttggt atcagtcgtg gcgcagcagc tgccatttgt gcctccccgg
     1021 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcag gcttccgaaa
     1081 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
     1141 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
     1201 atgaaatgtt agatgtcagt gaaatcacca ccactgcccc cgcgttttat gccattttgc
     1261 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
     1321 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
     1381 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
     1441 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
     1501 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1561 tgatgctggt attggtgatt ctgaccatgc tggcggcagt cacgaccggt tcaggcaatg
     1621 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1681 cggcgtattt gactattccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1741 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1801 aacgcacctc ggtgccggtg cttgttgggc tagtgattgt tatcgttgct acagagctga
     1861 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1921 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1981 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     2041 agtggtgcgg ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     2101 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggcagttc
     2161 gtctttgggt tcaccgcgag cataggcatt aaacatctca tcaatttgct tctggctggc
     2221 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     2281 tgtattgatt cacttgaagt acgaaaaaac cgggagaaca ttggattatt cgggatctga
     2341 tgggattaga tttggtgggg cttgcaagcc tgtagtacaa attttaggcg ttaaccaatg
     2401 aaacgcgaaa gatagtaaaa aattgctttt gtttcattga aaaaacgaaa aacaaaaaca
     2461 ctataaatca tttcaatcat agcttcaaaa aacgttcaaa accgataaca accaggctgt
     2521 catcaaatga ctcatatcac aaatcagctc atgccgttta ggtatgttac atgtgtgatt
     2581 atgtgaggtg aagtatgttt tagctggctc atggttgtta tacggctttt ttcgcatctt
     2641 gtggttccaa caaaggagct acaggctctt cctgtttatg aacaccatac attgacaaaa
     2701 tctcttggcg agtttctgga ggtaatttat gcagcgccaa agctgtttgt agtgtttggc
     2761 gatcaatacc cagcgttgcc aatagggtgt caacggagat ggaggggcgt tcttgcatta
     2821 atccggaaag cccatattta gccatgttta gctctatctc tcggacttca ttttctccta
     2881 gtttattgat taacagggag aacattttca atgtatcagc attcatttct tcgatagcaa
     2941 ttttttgcat agccggtatt gccgggatat attcaacccc aggcccgcga cgcccttctt
     3001 gtgaacgctt cggccatcct tctttctttg ctcgtttatg caccccctgt attgtcccag
     3061 gcattccatc cagttcagta atttgtttgg gtgtcatcca ctggtttatc tcatttgcgc
     3121 ttttcataaa ctaagtttac cagatttatc tatataaaaa tcaatggatt acaaaataaa
     3181 ccacattaac tgatataaac cggtttggtt tatttggtaa tgtataaaac agataatcaa
     3241 gacgaattat ttgttttggt aaccctttat catacgcaat atggttggtg tgcgaaagat
     3301 gagtaacgat tggcatcggg ccgacgtgat tgcggccctc aaaaaaacag gaacccctct
     3361 ggcaaaattg tccagacgtt caggttattc aaccgggacg cttgcaaatg cattagtcag
     3421 gccatggcct aaaggtgaac gcattattgc tgacgcctta aacatgaaac ctgaggaaat
     3481 ttggcctagt agatatctaa gggatcacaa tgagagagtg gatatcagca attgaatgca
     3541 tgggtttatc tggtttacct ggttcattaa tgggggttca caaaagagct cagagagaag
     3601 gatggctcaa gagaagaaga gaaggaatta gaggggttgc ttacgaatac catatatctt
     3661 ctcttcctaa cattgctcga gctgaagttt tgctccaaca aggaaaattt aaaaccagtc
     3721 agggatgttt tgaaatcgcc cgccccacgc tggaagctca tgattatgat cgtgaggcac
     3781 tatggagcaa atgggataaa gccagcgatt cccagcgcag cctggctgaa aaatggttgc
     3841 cctcaattca ggccacagac gaaatgctga accaggggat ttcaacgaaa acggcttttg
     3901 cgaccgttgc agggcattac caggtcagcg catccacttt gcgggacaag tattaccagg
     3961 tacagaagtt tgcgaagcct gactgggcgg ctgcacttgt tgatggacgt ggagcatccc
     4021 gtcgcaatgt tcacaaaagt gaatttgacg aggatgcctg gcagtttctg attgcagatt
     4081 atctgcgacc ggaaaaaccc gccttccgca aatgttatga gcgtctggaa ctggcagccc
     4141 gcgagcatgg ctggagtatt ccctcccgtg ccacggcctt tcgccggatt cagcaactgg
     4201 acgaggcaat ggttgttgcc tgtcgtgaag gtgaacatgc actgatgcat ctgataccgg
     4261 cacagcagcg aactgtggaa cacctggacg ccatgcagtg gatcaacggc gacggttatc
     4321 tgcataacgt ctttgtacgc tggtttaacg gtgatgtgat ccgtccgaaa acatggttct
     4381 ggcaggatgt gaaaacccga aaaattctgg gctggcgctg cgatgtgagc gagaacattg
     4441 attcaattcg cctctcgttc atggatgttg tgactcgcta cagtatcccg gaggattttc
     4501 acatcaccat tgataacacc cgtggtgctg cgaataaatg gctgacggga ggcgcgccca
     4561 atcgctaccg ctttaaggta aaagaggacg atccaaaagg actgttttta ctgatgggtg
     4621 cgaaaatgca ctggacaagc gttgttgccg gtaaaggctg gggccaggca aaacctgttg
     4681 aacgtgcttt cggtgttggt gggcttgagg aatacgttga taagcatccg gcactggctg
     4741 gcgcatatac ggggccaaat ccgcaggcaa aacctgataa ctatggcgac cacgctgttg
     4801 atgcagagct gtttctgaaa acctttgccg aaggtgtggc gatgttcaat gccagaacag
     4861 gccgtgaaac agaaatgtgc gggggcaaac tctcgtttga tgatgttttc gagcgtgaat
     4921 acgccagaac gattgtgcgt aagccaaccg aagaacaaaa acggatgctg ttactgcctg
     4981 ccgaggcggt gaacgtttca cgcaaaggcg agtttacgct taaagttggc ggctccctta
     5041 aaggcgcgaa aaacgtttat tacaacatgg cattaatgaa tgccggagtg aaaaaagttg
     5101 tggtcagatt tgatccacag cagttacaca gcacggttta ttgag
//
LOCUS       DAZPDE010000218         5141 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPDE010000218 DAZPDE010000000
VERSION     DAZPDE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.222"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1223
                     /gene="ipaB"
                     /locus_tag="ACHU0J_003689"
     CDS             <1..1223
                     /gene="ipaB"
                     /locus_tag="ACHU0J_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR1218174.1"
                     /translation="KKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            1186..2334
                     /gene="ipaC"
                     /locus_tag="ACHU0J_003690"
     CDS             1186..2334
                     /gene="ipaC"
                     /locus_tag="ACHU0J_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR1218175.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            2385..3383
                     /gene="ipaD"
                     /locus_tag="ACHU0J_003691"
     CDS             2385..3383
                     /gene="ipaD"
                     /locus_tag="ACHU0J_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR1218176.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            3392..>5141
                     /gene="ipaA"
                     /locus_tag="ACHU0J_003692"
     CDS             3392..>5141
                     /gene="ipaA"
                     /locus_tag="ACHU0J_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR1218177.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSK"
BASE COUNT         1892 a         1047 c          879 g         1323 t
ORIGIN      
        1 aaaagaaaaa attaagccgg gaagaaatac aactcactat caaaaaagac gcagcagtta
       61 aagacaggac attgattgag cagaaaaccc tgtcaattca tagcaaactt acagataaat
      121 caatgcaact cgaaaaagaa atagactctt tttctgcatt ttcaaacaca gcatctgctg
      181 aacagctatc aacccagcag aaatcattaa ccggacttgc cagtgttact caattgatgg
      241 caacctttat tcaactagtt ggaaaaaata atgaagaatc tttaaaaaat gatctggctc
      301 tattccagtc tctccaagaa tcaagaaaaa ctgaaatgga gagaaaatct gatgagtatg
      361 ctgctgaagt acgtaaagca gaagaactca acagagtaat gggttgtgtt gggaaaatac
      421 ttggggcact tttaactatc gttagtgttg ttgcagcagc tttttctgga ggagcctctc
      481 tagcactggc agctgttggt ttagctctta tggttacgga tgctatagta caagcagcga
      541 ccggcaattc cttcatggaa caagccctga atccgatcat gaaagcagtc attgaaccct
      601 taatcaaact cctttcagat gcatttacaa aaatgctcga aggcttgggc gtcgactcga
      661 aaaaagccaa aatgattggc tctattctgg gggcaatcgc aggcgctctt gtcctagttg
      721 cagcagtcgt tctcgtagcc actgttggta aacaggcagc agcaaaactt gcagaaaata
      781 ttggcaaaat aataggtaaa accctcacag accttatacc aaagtttctc aagaattttt
      841 cttctcaact ggacgattta atcactaatg ctgttgccag attaaataaa tttcttggtg
      901 cagcgggtga tgaagtaata tccaaacaaa ttatttccac ccatttaaac caagcagttt
      961 tattaggaga aagtgttaac tctgccacac aagcgggagg aagtgtcgct tctgctgttt
     1021 tccagaacag cgcgtcgaca aatctagcag acctgacatt atcgaaatat caagttgaac
     1081 aactgtcaaa atatatcagt gaagcaatag aaaaattcgg ccaattgcag gaagtaattg
     1141 cagatctatt agcctcaatg tccaactctc aggctaatag aactgatgtt gcaaaagcaa
     1201 ttttgcaaca aactactgct tgatacaaat aaggagaatg ttatggaaat tcaaaacaca
     1261 aaaccaaccc agattttata tacagatata tccacaaaac aaactcaaag ttcttccgaa
     1321 acacaaaaat cacaaaatta tcagcagatt gcagcgcata ttccacttaa tgtcggtaaa
     1381 aatcccgtat taacaaccac attaaatgat gatcaacttt taaagttatc agagcaggtt
     1441 cagcatgatt cagaaatcat tgctcgcctt actgacaaaa agatgaaaga tctttcagag
     1501 atgagtcaca cccttactcc agagaacact ctggatattt ccagtctttc ttctaatgct
     1561 gtttctttaa ttattagtgt agccgttcta ctttctgctc tccgcactgc agaaactaaa
     1621 ttgggctctc aattgtcatt gattgcgttc gatgctacaa aatcagctgc agagaacatt
     1681 gttcggcaag gcctggcagc cctatcatca agcattactg gagcagtcac acaagtaggt
     1741 ataacgggta tcggtgccaa aaaaacgcat tcagggatta gcgaccaaaa aggagcctta
     1801 agaaagaacc ttgccactgc tcaatctctt gaaaaagagc ttgcaggttc taaattaggg
     1861 ttaaataaac aaatagatac aaatatcacc tcaccacaaa ctaactctag cacaaaattt
     1921 ttaggtaaaa ataaactggc gccagataat atatccctgt caactgaaca taaaacttct
     1981 cttagttctc ccgatatttc tttgcaggat aaaattgaca cccagagaag aacttacgag
     2041 ctcaataccc tttctgcgca gcaaaaacaa aacattggcc gtgcaacaat ggaaacatca
     2101 gccgttgctg gtaatatatc cacatcagga gggcgttatg catctgctct tgaagaagaa
     2161 gaacaactaa tcagtcaggc cagcagtaaa caagcagagg aagcatccca agtatctaaa
     2221 gaagcatccc aagcgacaaa tcaattaata caaaaattat tgaatataat tgacagcatc
     2281 aaccaatcaa agaattcgac agccagtcag attgctggta acattcgagc ttaatatatc
     2341 caagagccat aataatatat ggctcttcct gtaaggaaat aaccatgaat ataacaactc
     2401 tgactaatag tatttccacc tcatcattca gtccaaacaa taccaacggt tcatcaaccg
     2461 aaacagttaa ttctgatata aaaacaacga ccagttctca tcctgtaagt tcccttacta
     2521 tgctcaacga cacccttcat aatatcagaa caacaaatca ggcattaaag aaagagcttt
     2581 cacaaaaaac gttgactaaa acatcgctag aagaaatagc attacattca tctcagatta
     2641 gcatggatgt aaataaatcc gctcaactat tagatattct ttccaggcac gaatatccaa
     2701 ttaataaaga cgcaagagaa ttattacatt cagctccgaa agaagccgag cttgatggag
     2761 atcaaatgat atctcataga gaactgtggg ctaaaattgc aaactccatc aatgatatta
     2821 atgaacagta tctgaaagta tatgaacatg ccgttagttc atatactcaa atgtatcaag
     2881 attttagcgc tgttctttcc agtcttgccg gctggatctc tcccggaggt aacgacggaa
     2941 actccgtgaa attacaagtc aactcgctta aaaaggcatt ggaagaactc aaggaaaaat
     3001 ataaagataa accgctatat ccagcaaata atactgttag tcaggaacaa gcaaataaat
     3061 ggcttacaga attaggtgga acaatcggca aggtatctca aaaaaacggg ggatatgttg
     3121 tcagtataaa catgacccca atagacaata tgttaaaaag cttagataat ctaggtggaa
     3181 atggcgaggt tgtgctagat aatgcaaaat atcaggcatg gaatgccgga ttctctgccg
     3241 aagatgaaac aatgaaaaat aatcttcaaa ctttagttca aaaatacagt aatgccaata
     3301 gtatttttga taatttagta aaggttttga gtagtacaat aagctcatgt acagatacag
     3361 ataaactttt tctccatttc tgaggtgcgc catgcataat gtaaataata ctcaagcgcc
     3421 aacattctta tataaggcaa cttcaccatc atcaacagaa tacagcgagt taaaaagcaa
     3481 aatatccgat atccatagtt cgcaaacttc tctaaaaaca ccagcatcag tgtctgaaaa
     3541 agaaaacttt gcaacgtctt ttaatcagaa atgtcttgat tttttatttt cttcctcagg
     3601 gaaagaagat gtgttaagaa gcatttattc caactcaatg aatgcgtatg ccaaaagcga
     3661 gattctcgaa ttttcaaatg ttttgtactc cttagtacat caaaatggtc ttaattttga
     3721 aaacgaaaag ggacttcaaa aaattgtcgc acagtattcg gaactaatta taaaagataa
     3781 attatcccaa gattctgcct ttggaccatg gtcggcaaag aataagaaac tccatcaatt
     3841 acgacaaaac attgagcaca gacttgcact attagcacaa caacacacat ctggtgaagc
     3901 tttatcattg ggacaaaaac tcctcaatac tgaagtatca tcatttatca agaataatat
     3961 tcttgctgaa ttaaagttaa gtaatgaaac tgtttcatct ctcaaactag atgatttagt
     4021 tgacgcacag gcaaaacttg cctttgatag tttgcgcaat caacgtaaaa atactattga
     4081 tagtaaagga tttggtatag gtaaactgtc aagagactta aatacagtag ccgtgtttcc
     4141 tgagctgttg agaaaagtcc ttaatgatat tttagaagat ataaaagatt cgcatcctat
     4201 ccaagatggc ctccctacac ctcccgaaga tatgccagat ggcggaccaa cccccggagc
     4261 caatgagaaa acatcccaac ctgtaattca ctatcatata aataatgata atagaactta
     4321 cgataataga gtttttgaca acagagtata tgacaatagc tatcacgaga acccagaaaa
     4381 tgatgcacag tctcctactt ctcagacaaa cgatctatta tcccgtaacg gaaactcatt
     4441 actaaatcca caaagagcac tagttcaaaa agtaacttcc gttctaccac actctatatc
     4501 agatactgtc cagacatttg caaataattc agctttagaa aaggctttca accatactcc
     4561 agataattcg gatggaatag gttcagacct gttaactacg agtagtcaag aaagatctgc
     4621 aaataactct ctttctcggg gacacaggcc tctgaacata cagaactctt caaccacccc
     4681 ccctctccac ccggaaggag tgacaagcag taatgataac tcatcagata caactaaaag
     4741 tagcgcttct ctttctcata gagtagcttc gcaaatcaat aaattcaact caaacactga
     4801 ttcaaaagta cttcagactg attttttatc aagaaatgga gacacatatt taacacggga
     4861 aacgatattt gaagcttcaa aaaaagtaac aaactcccta agtaatctta tatctctcat
     4921 tggaactaaa tcaggaacac aagaacgaga gttacaggaa aaatcaaagg acattacaaa
     4981 atccacaaca gaacatagaa taaacaacaa attaaaagtt acagatgcaa atataagaaa
     5041 ctacgtaaca gaaaccaacg cagatacaat tgataaaaat catgcgatct atgaaaaggc
     5101 aaaagaagta tctagcgccc tcagcaaggt attgtcaaaa a
//
LOCUS       DAZPDE010000219         5128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPDE010000219 DAZPDE010000000
VERSION     DAZPDE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.223"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHU0J_003693"
     CDS             <1..244
                     /locus_tag="ACHU0J_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218178.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHU0J_003694"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHU0J_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHU0J_003695"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHU0J_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1218179.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHU0J_003696"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHU0J_003696"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1218180.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHU0J_003697"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHU0J_003697"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1218181.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHU0J_003698"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHU0J_003698"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1218182.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1174 a         1301 c         1466 g         1187 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaata
//
LOCUS       DAZPDE010000220         5063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPDE010000220 DAZPDE010000000
VERSION     DAZPDE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.224"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHU0J_003699"
     CDS             complement(11..322)
                     /locus_tag="ACHU0J_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1218183.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHU0J_003700"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHU0J_003700"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHU0J_003701"
     CDS             701..1054
                     /locus_tag="ACHU0J_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1218184.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHU0J_003702"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHU0J_003702"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR1218185.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHU0J_003703"
     CDS             complement(2810..3280)
                     /locus_tag="ACHU0J_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1218186.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHU0J_003704"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHU0J_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHU0J_003705"
     CDS             complement(4119..4337)
                     /locus_tag="ACHU0J_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1218187.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHU0J_003706"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHU0J_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1218188.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1361 a         1172 c         1216 g         1314 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccg
//
LOCUS       DAZPDE010000221         5014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPDE010000221 DAZPDE010000000
VERSION     DAZPDE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.225"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHU0J_003707"
     CDS             <1..297
                     /locus_tag="ACHU0J_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1218189.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACHU0J_003708"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACHU0J_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1218190.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACHU0J_003709"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACHU0J_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1218191.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACHU0J_003710"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACHU0J_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1218192.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACHU0J_003711"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACHU0J_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1218193.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4379..>5014)
                     /locus_tag="ACHU0J_003712"
     CDS             complement(4379..>5014)
                     /locus_tag="ACHU0J_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1218194.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1323 a         1227 c         1200 g         1264 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt
     4201 tgtcgctgat aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt
     4261 gtgatttttg cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt
     4321 cttgcattat ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact
     4381 acgataaccg cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac
     4441 cgtaaatttc atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg
     4501 ggttcgctcc tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac
     4561 ggcggcgaat accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg
     4621 agtaataagg tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt
     4681 gtttttcagt tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc
     4741 cggtcagttt gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gctaccgtcc
     4801 ggatcccggc atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga
     4861 cattcacaat tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt
     4921 gacgaagccc ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt
     4981 ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DAZPDE010000222         4997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPDE010000222 DAZPDE010000000
VERSION     DAZPDE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.226"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            269..1213
                     /gene="leuO"
                     /locus_tag="ACHU0J_003713"
     CDS             269..1213
                     /gene="leuO"
                     /locus_tag="ACHU0J_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1218195.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1368..1412)
                     /locus_tag="ACHU0J_003714"
     CDS             complement(1368..1412)
                     /locus_tag="ACHU0J_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218196.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1531..3255
                     /gene="ilvI"
                     /locus_tag="ACHU0J_003715"
     CDS             1531..3255
                     /gene="ilvI"
                     /locus_tag="ACHU0J_003715"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1218197.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3258..3749
                     /gene="ilvN"
                     /locus_tag="ACHU0J_003716"
     CDS             3258..3749
                     /gene="ilvN"
                     /locus_tag="ACHU0J_003716"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1218198.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3761..3847
                     /locus_tag="ACHU0J_003717"
     CDS             3761..3847
                     /locus_tag="ACHU0J_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1218199.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3929..4933
                     /gene="cra"
                     /locus_tag="ACHU0J_003718"
     CDS             3929..4933
                     /gene="cra"
                     /locus_tag="ACHU0J_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1218200.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1215 a         1136 c         1314 g         1332 t
ORIGIN      
        1 ttaatccatt ttgtagatga ttgagtattc gcggtagtta tgattagatt gttttcgcaa
       61 caaaacatta tggattatta tgctgtggta aatgactcat tccacggcaa tggattctgt
      121 ttttatcaga acccgtatct ttatgttttc cgaattttac tcattttgct ttttcttatt
      181 ttatatgcat gataaatcat attcttcagg attatttctc tgcattccaa taagggaaag
      241 ggagttaagt gtgacagtgg agttaagtat gccagaggta caaacagatc attcagagac
      301 ggcggagtta agcaagccac agctacgcat ggtcgatctc aacttattaa ccgttttcga
      361 tgccgtgatg caggagcaaa acattacccg tgccgctcat gttctgggta tgtcgcaacc
      421 tgcggtcagt aacgctgttg cacgcctgaa ggtgatgttt aatgacgagc tttttgttcg
      481 ttatggccgt ggtattcaac cgactgctcg cgcatttcaa ctttttggtt cagttcgcca
      541 ggcattgcaa ctagtacaaa atgaattgcc tggttcaggt tttgaacccg cgagcagtga
      601 acgtgtattt catctttgtg tttgcagccc gttagacagc attctgacct cgcagattta
      661 taatcacatt gagcagattg cgccaaatat acatgttatg ttcaagtctt cattaaatca
      721 gaacactgaa catcagctgc gttatcagga aacggagttt gtgattagtt atgaagactt
      781 ccatcgtcct gaatttacca gcgtgccatt atttaaagat gaaatggtgc tggtagccag
      841 caaaaatcat ccaacaatta aaggcccgtt actgaaacat gatgtttata acgaacaaca
      901 tgcggcggtt tcgctcgatc gtttcgcgtc atttagtcaa ccttggtatg acacggtaga
      961 taagcaagcc agtatcgcgt atcagggcat ggcaatgatg agcgtactta gcgtggtgtc
     1021 gcaaacgcat ttggtcgcta ttgcgccgcg ttggctggct gaagagttcg ctgaatcctt
     1081 agaattacag gtattaccgc tgccgttaaa acaaaacagc agaacctgtt atctctcttg
     1141 gcatgaagct gccgggcgcg ataaaggcca tcagtggatg gaagaacaat tagtctcaat
     1201 ttgcaaacgc taactgaatg cagaataggc cagacatgaa tgtctggttt attctgcatt
     1261 ttttattgaa tgtagaattt tattctgaat gtgtggactc tctattttag gattaattaa
     1321 aaaaatagag aaattgctgt aagttgtggg attcagccga tttattatca atttaatcct
     1381 tcgtaatgga ggattttatc gtttcttttc acctttcctc ctgtttattc ttattaccct
     1441 gcgtttatgt ctctggctgc caattgctta agcaagatcg gacggttaat gtgttttaca
     1501 cattttttcc gtcaaacagt gaggcaggcc atggagatgt tgtctggagc cgagatggtc
     1561 gtccgatcgc ttatcgatca gggcgttaaa caagtattcg gttatcccgg aggcgcagtc
     1621 cttgatattt acgatgcatt gcataccgtg ggtggtattg atcatgtatt agttcgtcat
     1681 gagcaggcgg cggtgcatat ggccgatggc ctggcgcgtg cgaccgggga agtcggcgtc
     1741 gtgctggtaa cgtcgggtcc aagggcgacc aatgcgatta ccggcatcgc caccgcttat
     1801 atggattcca ttccattagt tgtcctttcc gggcaggtag cgacctcgtt gataggttac
     1861 gatgcctttc aggagtgcga catggtgggg atttcgcgac cggtggttaa acacagtttt
     1921 ctggttaagc aaacggaaga cattccgcag gtgctgaaaa aggctttctg gctggcggca
     1981 agtggtcgcc caggaccagt ggtcgttgat ttaccgaaag atattcttaa tccggcgaac
     2041 aaattaccct atgtctgccc tgagtcggtc agtatgcgtt cttacaatcc cactactacc
     2101 ggacataaag ggcaaattaa gcgtgctctg caaacgctgg tagcggcaaa aaaaccggtt
     2161 gtctacgtag gcggtggggc aatcatggcg ggctgccatc agcagttgaa agaaacggtg
     2221 gaggcgttga atctgcccgt tgtttcctca ttgatgggaa tgggggcgtt tccggcaacg
     2281 catcgtcagg cactgggcat gctgggaatg cacggtacct acgaagccaa tatgacgatg
     2341 cataacgcgg atgtgatttt cgccgtcggg gtacgatttg atgaccgaac gacgaacaat
     2401 ctggcaaagt actgcccaaa tgccactgtt ctgcatatcg atattgatcc tacttccatt
     2461 tctaaaaccg tgactgcgga tatcccgatt gtgggggatg ctcgccaggt cctcgaacaa
     2521 atgcttgaac tcctgtcgca agaatccgcc catcaaccgc tggatgagat ccgcgactgg
     2581 tggcagcaga ttgaacagtg gcgcgctctt cagtgcctga aatatgacac tcacagtgaa
     2641 aagattaaac cgcaggcggt gatcgagact ctttggcggt tgactaatgg ggacgcttac
     2701 gtgacgtccg atgtcgggca gcaccagatg tttgctgcgc tttattatcc attcgacaaa
     2761 ccgcgtcgct ggatcaattc cggcggtctc ggcacgatgg gttttggttt acctgcggca
     2821 ctgggcgtca aaatggcgtt gccagaagaa accgtggttt gcgtcactgg cgacggcagt
     2881 attcagatga acatccagga actgtctacc gcgttgcaat acgagttgcc cgtactggtg
     2941 gtgaatctca ataaccgcta tctggggatg gtgaaacagt ggcaggacat gatctattcc
     3001 ggccgtcatt cacaatctta tatgcaatca ctacccgatt tcgtccgtct ggcggaagcc
     3061 tatggtcacg tcgggatcca gatctctcat ccgcatgagc tggaaagcaa acttagcgag
     3121 gcgctggaac aggtgcgcaa taatcgcctg gtgtttgttg atgttaccgt cgatggcagt
     3181 gagcacgtct acccgatgca gattcgcggg ggcggaatgg atgaaatgtg gttaagcaaa
     3241 acggagagaa cctgattatg cgccggatat tatcagtctt actcgaaaat gaatcaggcg
     3301 cgttatcccg cgtgattggc cttttttccc agcgaggcta caacattgaa agcctgaccg
     3361 ttgcgccaac cgacgatccg acattatcgc gtatgaccat ccagaccgtg ggcgatgaaa
     3421 aagtacttga gcagatcgaa aagcaattac acaagctggt cgatgtcttg cgcgtgagtg
     3481 agttggggca gggcgcgcat gttgagcggg aaatcatgct ggtgaaaatt caggccagcg
     3541 gttacgggcg tgacgaagtg aaacgtaata cggaaatatt ccgtgggcaa attatcgatg
     3601 tcaccccctc gctttatacc gttcaattag caggcaccag cgataagctt gatgcatttt
     3661 tagcatcgat tcgcgatgtg gcgaaaattg tggaagttgc tcgctctggt gtggtcggac
     3721 tttcgcgcgg cgataaaata atgcgttgag aatgatctca gtgcgcaatt tgcagcccaa
     3781 catgtcacgt tgggcttttt ttgcgaaatc agtgggaacc tggaataaaa gcagttgccg
     3841 cagttaattt tctgcgctta gatgttaatg aatttaaccc ataccagtac aatggctatg
     3901 gtttttacat tttacgcaag gggcaattgt gaaactggat gaaatcgctc ggctggcggg
     3961 agtgtcgcgg accactgcaa gctatgttat taacggcaaa gcgaagcaat accgtgtgag
     4021 cgacaaaacc gttgaaaaag tcatggctgt ggtgcgtgag cacaattacc acccgaacgc
     4081 cgtggcagct gggcttcgtg ctggacgcac acgttctatt ggtcttgtga tccccgatct
     4141 ggagaacacc agctataccc gcatcgctaa ctatcttgaa cgccaggcgc ggcaacgggg
     4201 ttatcaactg ctgattgcct gctcagaaga tcagccagac aacgaaatgc gctgcattga
     4261 gcacctttta cagcgtcagg ttgatgccat tattgtttcg acgtcgttgc cacctgagca
     4321 tcctttttat caacgctggg ctaacgaccc gttcccgatt gtcgcgctgg accgcgccct
     4381 cgatcgtgaa catttcacca gcgtggttgg tgccgatcag gatgatgccg aaatgctggc
     4441 ggaagagtta cgtaagtttc ccgccgagac ggtgctttat cttggcgcgc tgccggagct
     4501 ttctgtcagc ttcctgcgtg aacaaggttt ccgtactgct tggaaagatg atccgcgcga
     4561 agtgcatttc ctgtatgcca acagctatga gcgggaggcg gctgcccagt tattcgaaaa
     4621 atggctggaa acgcatccga tgccgcaggc gctgttcaca acgtcgtttg cgttgttgca
     4681 aggagtgatg gatgtcacgc tgcgtcgcga cggcaaactg ccttctgacc tggcaattgc
     4741 cacctttggc gataacgaac tgctcgactt cttacagtgt ccggtgctgg cagtggctca
     4801 acgtcaccgc gatgtcgcag agcgtgtgct ggagattgtc ctggcaagcc tggacgaacc
     4861 gcgtaagcca aaacctggtt taacgcgcat taaacgtaat ctctatcgcc gcggcgtgct
     4921 cagccgtagc taatccgcga acaaaaatac gcgccaggtg aatttccctc tggcgcgtag
     4981 agtacgggac tggacat
//
LOCUS       DAZPDE010000223         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPDE010000223 DAZPDE010000000
VERSION     DAZPDE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.227"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHU0J_003719"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHU0J_003719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR1218201.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHU0J_003720"
     CDS             1020..1703
                     /locus_tag="ACHU0J_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR1218202.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHU0J_003721"
     CDS             1700..2161
                     /locus_tag="ACHU0J_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR1218203.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHU0J_003722"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHU0J_003722"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1218204.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHU0J_003723"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHU0J_003723"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR1218205.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHU0J_003724"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHU0J_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR1218206.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHU0J_003725"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHU0J_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR1218207.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHU0J_003726"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHU0J_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218208.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZPDE010000224         4874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPDE010000224 DAZPDE010000000
VERSION     DAZPDE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.228"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /locus_tag="ACHU0J_003727"
     CDS             complement(<1..214)
                     /locus_tag="ACHU0J_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR1218209.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            524..2296
                     /gene="aspS"
                     /locus_tag="ACHU0J_003728"
     CDS             524..2296
                     /gene="aspS"
                     /locus_tag="ACHU0J_003728"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1218210.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2414..2866
                     /gene="nudB"
                     /locus_tag="ACHU0J_003729"
     CDS             2414..2866
                     /gene="nudB"
                     /locus_tag="ACHU0J_003729"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1218211.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2895..3635
                     /locus_tag="ACHU0J_003730"
     CDS             2895..3635
                     /locus_tag="ACHU0J_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1218212.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3670..4191
                     /gene="ruvC"
                     /locus_tag="ACHU0J_003731"
     CDS             3670..4191
                     /gene="ruvC"
                     /locus_tag="ACHU0J_003731"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1218213.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4193..4795)
                     /locus_tag="ACHU0J_003732"
     CDS             complement(4193..4795)
                     /locus_tag="ACHU0J_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1218214.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1237 a         1187 c         1321 g         1129 t
ORIGIN      
        1 catcaaccgg ctgttttaat gcttcggcgt aatcggcaga ccagccaact cgcaccagaa
       61 acacgggctg accgctggcg cgaaatttcg ccgccagctt cccggcgcga ttaactacct
      121 catcggcagt atgtggacca ccggcaaaag gtaagatgcc ttcttgtaaa tcgatcacca
      181 ccaacgcagt ggttttagca ttaagttcaa gcatgctgac tcctgaaaaa acaaaatcgt
      241 gcctcacacc ttaatgcgat tagccgatgg cggaacgaca aattttgtta atttttatga
      301 gttcgggcaa cacctttata gcaaactggc gtagagtctg atttcaggcc cgatataaag
      361 tggtaacgag gcgttccagc gcgggccagg ctcttacggt gcggcagaat ttccagtata
      421 atagccgcct tttttcattc agttgtgaca tacagctaac gctgcgactt ggtcacctgc
      481 gcagcagggg gcatccgccg gcagctaagt taagggatat ctcatgcgta cagaatattg
      541 tggacagctc cgtttgtccc acgtggggca gcaggtgact ctgtgtggtt gggtcaaccg
      601 tcgtcgtgat cttggtagcc tgatcttcat cgatatgcgc gaccgcgaag gtatcgtgca
      661 ggtatttttc gatccggatc gtgcggacgc gttaaagctg gcctctgaac tgcgtaatga
      721 gttctgcatt caggttacgg gcaccgtacg tgcgcgtgac gaaaaaaata ttaaccgcga
      781 tatggcgacc ggcgaaattg aagtgctggc gtcctcgctg actatcatca accgcgcaga
      841 tgttctgccg cttgactcta accacgtcaa caccgaagaa gcgcgtctga aataccgcta
      901 cctcgacctg cgtcgcccgg aaatggcgca acgcctgaaa acccgcgcta aaatcaccag
      961 cctggtgcgc cgttttatgg atgaccacgg cttcctcgac atcgaaactc cgatgctgac
     1021 caaagccacg ccggaaggcg cgcgtgacta cctggtgcct tctcgtgtgc acaaaggtaa
     1081 attctacgca ctgccgcaat ccccacagtt gttcaaacag ctgctgatga tgtccggttt
     1141 tgaccgttac tatcagatcg ttaaatgctt ccgtgacgaa gacctgcgcg ctgatcgtca
     1201 gcctgaattt actcagatcg atgtggaaac ttctttcatg accgcgccgc aagtgcgtga
     1261 agtgatggaa gcgctggtgc gtcatttgtg gctggaagtg aagggcgtgg atctgggcga
     1321 tttcccggta atgacctttg cggaagcaga acgccgttat ggttctgata aaccggatct
     1381 gcgtaacccg atggaactga ccgacgttgc cgatctgctg aaatctgttg agtttgcagt
     1441 atttgcgggg ccagcgaacg atccgaaagg ccgtgttgcc gctctacgtg ttccgggtgg
     1501 cgcatcgctg acccgtaagc agatcgacga atacggtaac ttcgttaaaa tctacggcgc
     1561 gaaaggtctg gcttacatca aagttaacga acgcgcgaaa ggtctggaag gtatcaacag
     1621 cccggtagcg aagttcctta atgcagaaat catcgaagcc atcctggatc gtactgccgc
     1681 gcaagatggc gatatgattt tcttcggtgc cgacaacaag aaaattgttg ccgacgggat
     1741 gggcgcactg ctcctgaaag tgggtaaaga ccttggtctg accgacgaaa gcaaatgggc
     1801 accgctgtgg gttatcgact tcccgatgtt tgaagacgac ggtgaaggcg gcctgacggc
     1861 aatgcaccat ccgttcacct caccgaaaga tatgactgct gcagaactga aagctgcacc
     1921 ggaaaatgcg gtggcgaacg cttacgatat ggtcatcaat ggttacgaag tgggcggtgg
     1981 ttcagtacgt atccataatg gtgatatgca gcagacggtg tttggtattc tgggtatcaa
     2041 cgaagaggaa cagcgcgaga aattcggctt cctgctcgac gccctgaaat acggtactcc
     2101 gccgcacgca ggtctggcat tcggtcttga ccgtctgacc atgctgctga ctggcaccga
     2161 caatatccgt gacgttatcg ccttcccgaa aaccacggcg gcagcgtgtc tgatgactga
     2221 agcaccgagc tttgctaacc cgactgcact ggctgagctg agcattcagg ttgtgaagaa
     2281 ggctgagaat aactgatatg actcaaatac acgaaatcat tcgcgttgca tcaaggcggc
     2341 aactgagtga actcccatga gcatagataa ctatgtgaat gggatgagcg aaggcagtca
     2401 acgaagaggc agcgtgaagg ataaagtgta taagcgtccc gtttcgatct tagtggtcat
     2461 ctatgcacaa gatacgaaac gggtgctgat gttgcagcgg cgtgacgatc ccgatttctg
     2521 gcagtcggta accggcagcg tggaagaggg tgaaaccgcg ccgcaagctg ccatgcgcga
     2581 agtaaaggaa gaggttacca ttgatgttgt cgctgaacaa ctgaccttaa ttgactgtca
     2641 gcgcacggta gagtttgaaa ttttttcaca tttacgtcat cgctatgcgc cgggcgtgac
     2701 gcgtaatacg gaatcatggt tctgtcttgc gcttccgcac gagcggcaga tcgttttcac
     2761 tgaacatctg gcttacaagt ggcttgatgc gcctgctgcg gcggcgctca ctaagtcctg
     2821 gagcaaccgg caggcgattg aacagtttgt aattaacgct gcctgaacag gcagggctat
     2881 ctggagatat ttttatggca ggtcatagta aatgggccaa caccagacat cgtaaagctg
     2941 cgcaggatgc taagcgcggt aaaatcttca ctaaaatcat tcgtgagctg gtaaccgcgg
     3001 ctaagctggg cggtggcgat ccggacgcta acccgcgtct gcgtgcggcg gtggataaag
     3061 cactgtctaa caacatgacc cgtgacacac tgaaccgcgc aattgcacgt ggtgtgggcg
     3121 gtgatgatga tgcaaacatg gaaaccatca tctacgaagg ttacggtcct ggcggcacgg
     3181 caatcatgat tgaatgtctg tctgacaacc gcaaccgtac cgttgctgaa gtgcgtcacg
     3241 cattcagcaa atgtggcggt aacctcggca ctgacggttc cgtagcttat ctgttcagca
     3301 aaaaaggcgt gatctccttc gagaaaggcg acgaagacac catcatggaa gcagctctgg
     3361 aagcaggtgc tgaagacgtt gtgacatatg atgacggcgc gattgatgtc tacactgcat
     3421 gggaagaaat gggtaaagtg cgcgacgctc tggaagcggc aggtctgaaa gcagacagcg
     3481 cggaagtttc catgatcccg tccaccaaag ctgatatgga tgcagaaacc gcgccgaaac
     3541 tgatgcgtct gatcgatatg ctggaagatt gcgacgacgt gcaggaagtt taccataacg
     3601 gtgaaatctc tgatgaggtt gcagcgactc tctgatgatg cctgctaaac agcaaaacgg
     3661 agacgcgtga tggctattat tctcggcatt gacccgggtt cgcgcgtgac cggctacggc
     3721 gtcatccgcc aggtaggtag gcaactgtcc tacctgggta gcggatgcat acgcaccaaa
     3781 gtggatgatt taccgtctcg tctgaagctc atctatgcgg gcgtgacgga aatcatcacc
     3841 cagttccagc ctgattattt cgccattgaa caagtcttta tggcgaagaa cgctgactca
     3901 gccctgaaac tgggccaggc gcgcggcgtg gcgattgttg cggcggtgaa tcaggaattg
     3961 ccggtatttg aatacgcggc acgtcaggta aagcaaacgg tggtaggtat gggtagtgcc
     4021 gaaaaaagcc aggtgcagca tatggtccgc accttgctga aactgcccgc taatccacag
     4081 gcggatgccg ccgatgcgct ggcgattgcc atcacccact gccacgttag tcagaatgcg
     4141 atgcagatga gcgaatcgcg gctgaacctg acgagagggc gactgcgtta agttataccg
     4201 cctcggtcag ttccggctga ggcgtttcaa ctccctccgc gtgaatcaac accaaacccg
     4261 gatgatgcca caaactggct ggcgtgacgg ttttacgctc ccacggaata gaacctaaga
     4321 accagaattt ccagaaagtg agttttgcat tcggattgct atttaacagt tgtccaaacg
     4381 tctctatttc acccaccgga atacatagcg cctctttata aatatcaaag acaaattttg
     4441 aacagaactg gcgcgaagat tcgtatttaa aacccgtgtg gtaaagtttg cgtagccggg
     4501 agggaacctg ttcaacaatt cgttgttttt gttgttctgt cagcccagcg tctaatcgct
     4561 ttatagcata gcgttgatta gacgaacgtt taataaaacg agatagcgtg gtgatggttg
     4621 aaaggggaac ccggctttct gcaaccagga agtcttcacc gttatgaccg ataatgatcc
     4681 cgacgtgatt actccagcaa tttgatgcag ctgatatttg accaaataag gcagcaccta
     4741 tacatgtaaa gacgatatct ccaatttcat attcagcagg gtaattaata ttcacgttgt
     4801 cattccattg aaatagatac aaatatacgt aatagcataa aaaaattatt tttcaggagt
     4861 cgcaatgtat attt
//
LOCUS       DAZPDE010000225         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPDE010000225 DAZPDE010000000
VERSION     DAZPDE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.229"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHU0J_003733"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHU0J_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1218215.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHU0J_003734"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHU0J_003734"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR1218216.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHU0J_003735"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHU0J_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHU0J_003736"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHU0J_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1218217.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHU0J_003737"
     CDS             4169..4342
                     /locus_tag="ACHU0J_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218218.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHU0J_003738"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHU0J_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1218219.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHU0J_003739"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHU0J_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1218220.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHU0J_003740"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHU0J_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZPDE010000226         4832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPDE010000226 DAZPDE010000000
VERSION     DAZPDE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.230"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(18..779)
                     /gene="kduD"
                     /locus_tag="ACHU0J_003741"
     CDS             complement(18..779)
                     /gene="kduD"
                     /locus_tag="ACHU0J_003741"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1218221.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(809..1645)
                     /gene="kduI"
                     /locus_tag="ACHU0J_003742"
     CDS             complement(809..1645)
                     /gene="kduI"
                     /locus_tag="ACHU0J_003742"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1218222.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(1932..3113)
                     /locus_tag="ACHU0J_003743"
     CDS             complement(1932..3113)
                     /locus_tag="ACHU0J_003743"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1218223.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3368..4597
                     /locus_tag="ACHU0J_003744"
                     /pseudo
     CDS             3368..4597
                     /locus_tag="ACHU0J_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         1269 a         1223 c         1042 g         1298 t
ORIGIN      
        1 aaagtaagag gaatgaatta acgcgccagc caaccgccat ccacagcaat ggtataacca
       61 tttacataat ctgaagcgct ggaggcaaga aacactaccg gccccatcag gtcactcggc
      121 agtccccaac ggccagctgg aatgcgatcg agaatttccg cgctacgttg ttcatctgcc
      181 cgcagttgtt gggtattgtt ggtcgccatg taacccggag ctatcgcatt aacattaatg
      241 ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac ccatcacgcc gctttttgat
      301 gcggtataag aaggcacacg gatcccgccc tggaaggaga gcattgacgc gatattgata
      361 atcttgccgc cattgccttg cgcgataaag tgtttcgccg ctgcctgaga catgaagaat
      421 acgctcttga tattcaggtt catgacatcg tcccagtctt tttcgctgaa ctcgagagcg
      481 tcttcgcggc gaatcaatcc ggcgttattc accaggatat caatatgacc aaattcagct
      541 accgcgcgat ccagcagtcc aggaatgcca tcaatctttc gcagatcggc ggtcaggctt
      601 aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt cagtcggttc aacgatgtta
      661 atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg gcccagccca
      721 gtatcacaac cagtgacgac cgcaacttta ccttggagag aaaatgcact taaaatcata
      781 acaacacctt attcgtttat gcccacaact agcgcaaatc tttaacgccc acatggtcca
      841 tatcattaaa gacctggttt tcaccgacca tgccccagat aaaggtataa gctttggttc
      901 cgacgccgga atggatcgac cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa
      961 tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca tcatccatat
     1021 tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg acacggcatg gtgttccaca
     1081 agttacctgg agccagctcc gtcagcccca tactcaattg acaggtttcc agtacatccg
     1141 ggacaaaata tttgttaatc gtgcgacggt tactggtgag gttatcgcct aacgtgactg
     1201 gagatacttc gtccggtgtg acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat
     1261 tgtaataaaa cttcgccgga gtggcggtat caatactggc aaagacaact tcttttgcac
     1321 ctttaccaac atacagggcg tcgcggtgac cgatttcata gcattgacca tcgacagtaa
     1381 tcgtaccggc accgccaata ttgataacac ctaactcgcg acgttcaagg aaatagctta
     1441 cgccgagttg tttaccaact tccccgccaa cggaaaccgt tttagttatc ggcataatgc
     1501 cgccaacaat aattcggtca atgtggctgt aaaccatggt gtactcatcg gcgacaaata
     1561 ccttttcaac caaaaattca ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac
     1621 cgtggatgct ctgtcttacg tccacatcaa cctccgataa tcagtgaagc gtgaaaatag
     1681 aaaacgatag tgaaacactg tttcgttttc tctataaagg gatgatatgc ccatgggcta
     1741 gggttttcaa ttaaaaataa aacaatgttt tatttttaat ttaaggatta aaaaaagtgc
     1801 agtgtcgatc acgaatatgt catgagaatg gaaaaatgag ggagcccaga aaatgtatgt
     1861 cgacttgcca gccatcatta ttcaacagat taatgacggc agatatttat cggtaaggag
     1921 gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca cgccctggcc cccgccgata
     1981 caaagcgttg ccagcccttt gcgggcatta cgtttcacca tttcatgaac cagagaaacc
     2041 aggattcggc aaccggaagc gcctatcggg tgaccgagtg cgatcgcgcc accattgaca
     2101 ttgacccgac gctcatccca ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac
     2161 gcttcattag cctcgataag atcgacatca gccaactgcc agcctacacg ctccaggcaa
     2221 cggcgggtcg catacaccgg cgcaattccc atcaatgccg gatctacacc aacgctggca
     2281 aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc
     2341 atcattaccg cagctgcgcc atcgtttatg gatgatgcat tccccgcggt cacagaaccg
     2401 agactatcaa atgaaggatt taaacgggct aaaccttctg cactggcgtc agtgcgtggc
     2461 tgttcatcgg tatcaacaac caacgtctga ccgttacttt ggatcattac cgggacgatc
     2521 tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg
     2581 taagcatcct gcaactgacg gctgatgcca tattcgcgag ccagattttc ggcggtgaca
     2641 ccaatatgat aatcattgaa ggcatcccac aacccatcat gcaccagact gtcaaccaac
     2701 tggctattgc caagctgttc accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc
     2761 atgttttcct ggccacctgc gatgacaata tcagcctcgc cacactgtat cgcctgagta
     2821 gccagatgca gtgctttaag accggaaccg caaacgtcat taatcgtgat tgcagaaacg
     2881 ctgttaggaa gaccaccttt aatagccgat tgccttgccg gattctgccc tgcccctgca
     2941 gtcaacacct gaccaagaat tacttcatcc accgcatatg caggaacgcc ggtacgttct
     3001 attaacgctt tcacgaccag actaccaagt tccacggcgg aatgacccgc taacgcacca
     3061 cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca caacgtcttt catcacaacc
     3121 tcgcaccaat taacagtgca atagtaaatg attgttaatt acagttatct taattgttta
     3181 aaaaaagtga tttttatcac aaaggaaata tgcctgagca gtagtcagag tcataactgg
     3241 cacgtaaggt ttgcaaccac taacccacca atagatgggt agatagggcg ttaatctccc
     3301 atacttaact tggtttatgg taaattgccc tccattttgt ttaatttgta gatgatacgt
     3361 tcagataatg tctaatattt ggtctaaaga agaaactctg tgaagtttcg cgctctatgg
     3421 cacagccgtt gatgcaggca cgctattcct tcctattcag ttaggttcgg caggggctgt
     3481 ggtcctgttt attactgctc tggtcgcctg gcctttaaca tattggccac ataaagcctt
     3541 atgccagttc atcctctcat caaaaacatc agcaggtgaa gggataacgg gcgcggtaac
     3601 acactactat ggcaagaaga ttggtaatct gattaccacg ctgtacttca tcgccttttt
     3661 tgtcgtcgtg ttgatatatg cagtggcaat taccaactca cttacggaac agctggcaaa
     3721 gcatatggtt attgatcttc gcatccgtat gttggtgagt ctgggtgttg tattaattct
     3781 gaatctcatt tttctgatgg gacgtcatgc cactattcgg gtaatgggat ttttggtatt
     3841 cccattgatt gcctatttct tatttctttc catttacctt gtcggtagtt ggcaacctga
     3901 tctattaaca acccaggtag agttcaatca gaataccctt caccagatat ggatatcgat
     3961 tcccgtgatg gttttcgcct ttagccatac gcccattatt tctacgtttg ccatagacag
     4021 acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa aaaattatga aagtcgctta
     4081 tctcatcatc tgcataagtg tactgttctt tgtctttagc tgcctgcttt ctattccatc
     4141 ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt ttatcggcgc tttctatgct
     4201 gccgaacgcc ccagcatggt tgtcaatttc cgggattatt gtcgcagtag ttgcgatgtc
     4261 gaaatcattc ctgggtacgt actttggcgt tattgaaggt gccacagagg tcgtcaaaac
     4321 aacactacag caggttggtg taaagaaaag tcgtgcattt aaccacgcac tatcaattat
     4381 gttggtatcg ctgattacct tcattgtttg ttgcattaac ccgaacgcga tttcgatgat
     4441 ttacgcgatc agcggcccgc tcattgccat gatacttttc atcatgccta cgctgtcaac
     4501 gtatctcatc ccggcgctta aaccctggcg ttccatcgga aatctgatta cgctgatcgt
     4561 gggtatcctg tgcgtatcgg taatgttctt tagctaatat ccgttgtgga tggttcaaaa
     4621 ttgctacaat tttgaaccat tccatttttc ataatattca tttgttatta ttccgcccta
     4681 tcacttctct cgtcatatcc tagaattatc agttagttaa acacacaaag agatctgaac
     4741 atcttaaata agactattta agatgcataa cttagattcg caagatatct tctggtcatt
     4801 caaaaacaat ttgcgatata gatattctca ta
//
LOCUS       DAZPDE010000227         4829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPDE010000227 DAZPDE010000000
VERSION     DAZPDE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.231"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..944
                     /locus_tag="ACHU0J_003745"
     CDS             <1..944
                     /locus_tag="ACHU0J_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1218224.1"
                     /translation="NQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVN
                     AAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGA
                     LDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQ
                     TDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLLPEGQ
                     NFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKL
                     PFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            1153..2118
                     /gene="lipA"
                     /locus_tag="ACHU0J_003746"
     CDS             1153..2118
                     /gene="lipA"
                     /locus_tag="ACHU0J_003746"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1218225.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHU0J_003747"
     CDS             complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHU0J_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1218226.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2551..3339)
                     /locus_tag="ACHU0J_003748"
     CDS             complement(2551..3339)
                     /locus_tag="ACHU0J_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1218227.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            3432..3815
                     /gene="crcB"
                     /locus_tag="ACHU0J_003749"
     CDS             3432..3815
                     /gene="crcB"
                     /locus_tag="ACHU0J_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1218228.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHU0J_003750"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHU0J_003750"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1218229.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4253..4813)
                     /gene="pagP"
                     /locus_tag="ACHU0J_003751"
     CDS             complement(4253..4813)
                     /gene="pagP"
                     /locus_tag="ACHU0J_003751"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1218230.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1280 a         1221 c         1083 g         1245 t
ORIGIN      
        1 ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta
       61 caaccttacg aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata
      121 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
      181 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
      241 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
      301 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
      361 ctccctcggt cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc
      421 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
      481 aaaccgatct catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
      541 tgttcaccga caatatggtg ttaatttgcc gcaagggaaa tccactactc tctttagaag
      601 atgacagaga gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt
      661 tcagcggtct gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca
      721 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
      781 ccagccgttt ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt
      841 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcatc ctgcgtgatc
      901 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
      961 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
     1021 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
     1081 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
     1141 aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc
     1201 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
     1261 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
     1321 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
     1381 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc
     1441 cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca
     1501 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
     1561 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
     1621 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg
     1681 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
     1741 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
     1801 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
     1861 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
     1921 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
     1981 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
     2041 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
     2101 ggaatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
     2161 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
     2221 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
     2281 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
     2341 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
     2401 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
     2461 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
     2521 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
     2581 gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
     2641 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
     2701 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
     2761 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac
     2821 ccaggcggca ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc
     2881 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa
     2941 cggagctatt tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata
     3001 gagatgcagt ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt
     3061 ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg
     3121 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     3181 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga
     3241 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     3301 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
     3361 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
     3421 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     3481 tagcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     3541 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     3601 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     3661 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     3721 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     3781 cattctggct gttttcggcc tcaaccgtac actaaagacc aaaaaaaacc cgctgattaa
     3841 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     3901 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     3961 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     4021 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     4081 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg
     4141 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
     4201 gttgcttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
     4261 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
     4321 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
     4381 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
     4441 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
     4501 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
     4561 aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
     4621 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
     4681 gctgttgcca ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag
     4741 caaaaacttt accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt
     4801 tactcacgtt catttgtgac cataaaaca
//
LOCUS       DAZPDE010000228         4688 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPDE010000228 DAZPDE010000000
VERSION     DAZPDE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.232"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHU0J_003752"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHU0J_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR1218231.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHU0J_003753"
     CDS             2739..4208
                     /locus_tag="ACHU0J_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1218232.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>4688
                     /locus_tag="ACHU0J_003754"
     CDS             4208..>4688
                     /locus_tag="ACHU0J_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1218233.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     "
BASE COUNT         1357 a         1150 c         1133 g         1048 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatc
//
LOCUS       DAZPDE010000229         4685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPDE010000229 DAZPDE010000000
VERSION     DAZPDE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.233"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1132
                     /gene="proV"
                     /locus_tag="ACHU0J_003755"
     CDS             <1..1132
                     /gene="proV"
                     /locus_tag="ACHU0J_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1218234.1"
                     /translation="IEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKS
                     TMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDN
                     TAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPD
                     ILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGE
                     VVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSAL
                     KLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLSE
                     LLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1125..2189
                     /gene="proW"
                     /locus_tag="ACHU0J_003756"
     CDS             1125..2189
                     /gene="proW"
                     /locus_tag="ACHU0J_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1218235.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2247..3239
                     /gene="proX"
                     /locus_tag="ACHU0J_003757"
     CDS             2247..3239
                     /gene="proX"
                     /locus_tag="ACHU0J_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1218236.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3431..4610
                     /locus_tag="ACHU0J_003758"
                     /pseudo
     CDS             3431..4610
                     /locus_tag="ACHU0J_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1035 a         1237 c         1262 g         1151 t
ORIGIN      
        1 tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa actgggctat cgcttggcgt
       61 aaaagacgcc agtctggcca ttgaagaagg cgagatattt gtcatcatgg gattatccgg
      121 ctcgggtaaa tccacaatgg tacgccttct caatcgcctg attgaaccca cccgcgggca
      181 agtgctgatt gatggtgtgg atattgccaa aatatccgac gccgaactcc gtgaggtgcg
      241 cagaaaaaag attgcgatgg tcttccagtc ctttgcctta atgccgcata tgaccgtgct
      301 ggacaatact gcgttcggta tggaattggc cggaattaat gccgaagaac gccgggaaaa
      361 agcccttgat gcactgcgtc aggtcgggct ggaaaattat gcccacagct acccggatga
      421 actctctggc gggatgcgtc aacgtgtggg attagcccgc gcgttagcga ttaatccgga
      481 tatattatta atggacgaag ccttctcggc gctcgatcca ttaattcgca ccgagatgca
      541 ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc attgtcttta tttcccacga
      601 tcttgatgaa gccatgcgta ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt
      661 acaggtcggc acaccggatg aaattctcaa taatccggcg aatgattatg tccgtacctt
      721 cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa gatattgccc gccggacacc
      781 gaatggctta attcgtaaaa ctcctggctt cggcccacgt tcggcactga aattattgca
      841 ggatgaagat cgcgaatatg gctacgttat cgaacgcggt aataagtttg tcggcgcagt
      901 ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat
      961 tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc gagttgctct ctcatgtcgg
     1021 acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc
     1081 gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta aataatggct gatcaaaata
     1141 atccgtggga taccacgcca gcggcggaca gtgccgcgca atccgcagac gcctggggta
     1201 caccgacgac tgcaccggct gacggcggtg gtgctgactg gctgaccagt acgcctgcgc
     1261 caaacgtcga gcattttaat attctcgatc cgttccataa aacgctgatc ccgctcgaca
     1321 gttgggtcac tgaagggatc gactgggttg ttacccattt ccgtcccgtc ttccagggcg
     1381 tgcgcgttcc ggttgattat atcctcaacg gtttccagca attgctgctg ggtatgccag
     1441 cgccagtggc gattatcgtt ttcgctctca tcacctggca gatttccggg gtcggaatgg
     1501 gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcaa
     1561 tggtgactct ggcgctggtg ttaactgccc tgctgttctg tatcgtcatc ggtttgccgt
     1621 tggggatatg gctggcgaga agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg
     1681 ccatgcagac cacgccagcg tttgtttatc tggtgccaat cgtcatgcta tttggtatcg
     1741 gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct gccgccgatt atccgtctga
     1801 ccattctggg gattaaccag gttccggcgg atctgattga agcctcgcgc tcattcggtg
     1861 ccagcccgcg ccagatgctg ttcaaagttc agttaccgct ggcgatgccg accattatgg
     1921 cgggcgttaa ccagacgctg atgctggccc tttctatggt ggtcattgcc tcgatgattg
     1981 ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg tcgtctggat atggggcttg
     2041 ccaccgttgg cggcgtcggg attgtgatcc tcgccattat cctcgatcgc ctgacgcagg
     2101 ccgttgggcg cgactcacgc agtcgcggca accgtcgctg gtacaccact ggccctgttg
     2161 gtctgctgac ccgcccattc attaagtaac tctgcacttg cccggtgacg ccgggcatta
     2221 tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt actttttgcg acagcgtttg
     2281 ccacgcttat ctctacacaa acttttgctg ccgatctgcc gggcaaaggc attactgtta
     2341 atccagttca gagcaccatc actgaagaaa ccttccagac gctgctggtc agtcgtgcgc
     2401 tggagaaatt aggttatacc gtcaacaaac ccagcgaagt ggattacaac gttggctaca
     2461 cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa ctggacgcca ctccatgaca
     2521 acatgtacga agctgccggt ggcgataaga aattttatcg tgaaggggta tttgttaacg
     2581 gcgcagcaca gggttacctg atcgataaga aaaccgccga ccaatacaaa atcaccaaca
     2641 tcgcacaact gaaagatccg aagatcgcca aactgttcga taccaacggc gacggtaaag
     2701 cggatttaac cggttgtaac cctggctggg gctgcgaagg tgcgatcaac caccagcttg
     2761 ccgcgtatga actgaccaac accgtgacgc ataatcaggg gaactacgca gcgatgatgg
     2821 ccgacaccat cagtcgctac aaagagggca aaccggtgtt ttattacacc tggacgccgt
     2881 actgggtgag taatgagctg aagccaggga aagatgtggt ctggttgcag gtgccgttct
     2941 ccgcactgcc gggcgataaa aatgccgata ccaaactgcc gaatggcgcg aattacggct
     3001 tcccggtcag caccatgcat atcgttgcca acaaagcctg gaccgagaaa aatccggcgg
     3061 cagccaaact gtttgccatt atgcagttgc cagtggccga tattaacgcc cagaacgcca
     3121 ttatgcatga cggcaaagcc tcagaaggcg atattcaggg ccacgttgat ggctggatca
     3181 aagcccacca gcagcagttc gatggctggg tgaatgaggc gctggcagcg cagaagtaat
     3241 ttttattcgg gcggataagg cgttttcgcc tcatccgccg ctctgtacac aatgcctgat
     3301 gcgacgctga cgcgtcctat catgcctaca aaccacatcg cacatttcaa caagctatct
     3361 ttcatcccat attcatcaac atccgctatt attgatttcc agcttaatca tcacctgatg
     3421 aacaaaaata atgactaaac ctaatcatga gcttagcccg gcgctgatcg tgctgatgtc
     3481 tatcgccacc ggtctggcgg tagccagtaa ctattacgcc cagccattgc tcgacaccat
     3541 cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt attgttaccg ccgcgcagtt
     3601 gggctatgcc gcaggtctac tgtttcttgt tcccctcggt gatatgtttg aacgccgccg
     3661 cctgattgtc tcgatgacct tactggcagc gggcggtatg ttaatcactg ccagcagtca
     3721 gtcactggcg atgatgatcc tcggtacggc attaaccggt ttattctcag tcgtggcaca
     3781 aattctggtt ccgctggcag cgacgctggc ttcaccggat aaacgcggca aagtggttgg
     3841 caccattatg agcggtctgc tgttggggat attgctggca cgaaccgttg ccgggttact
     3901 ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct tcggtgttaa tggcactgat
     3961 ggcgctggca ttatggcgtg gtctgccaca aatgaaatca gaaacccacc tcaactaccc
     4021 acagttgttg ggttccgttt tcagcatgtt catcagcgat aagatcctgc gcacccgcgc
     4081 gttgctgggc tgcatgacct ttgccaactt cagcattctc tggacctcaa tggccttttt
     4141 gcttgccgct ccacctttta actacagcga tggtgacatt ggtctgtttg ggcttgcggg
     4201 agctgccgga gcgttgggcg ctcgtccggc gggcggtttt gccgacaagg gcaaatcgca
     4261 ccacaacttt cggtctgctt ctgctgttac tttcatggct ggcaatctgg tttggtcaca
     4321 cttccgtact ggcgttgatt atcggcattc tggtgctgga cctcaccgta cagggcgtgc
     4381 atatcactaa ccagacggta atttatcgaa tacatcctga tgcgcgtaat cgcctgaccg
     4441 caggttacat gaccagctac tttattggcg gtgccgccgg ttcgctaatt tcagcctcag
     4501 cctggcaaca tggcggttgg gctggcgttt gtctggctgg cgcgacgatt gccctggtta
     4561 acttactggt ctggtggcga ggttttcatc gtcaggaagc cgcaaattaa gcaatcagca
     4621 accgttttcg tggctttaca cttataaggg tgttaagaag cccatcagtc tgataaggtt
     4681 aagat
//
LOCUS       DAZPDE010000230         4669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPDE010000230 DAZPDE010000000
VERSION     DAZPDE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.234"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..615
                     /locus_tag="ACHU0J_003759"
     CDS             <1..615
                     /locus_tag="ACHU0J_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1218237.1"
                     /translation="VLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSGIPE
                     RIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKEDYDS
                     SLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVVKPS
                     DWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            618..2144
                     /locus_tag="ACHU0J_003760"
     CDS             618..2144
                     /locus_tag="ACHU0J_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR1218238.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            2145..3761
                     /locus_tag="ACHU0J_003761"
     CDS             2145..3761
                     /locus_tag="ACHU0J_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR1218239.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3817..3969)
                     /locus_tag="ACHU0J_003762"
                     /pseudo
     CDS             complement(3817..3969)
                     /locus_tag="ACHU0J_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3992..4369)
                     /locus_tag="ACHU0J_003763"
     CDS             complement(3992..4369)
                     /locus_tag="ACHU0J_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218240.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         1344 a          925 c         1151 g         1249 t
ORIGIN      
        1 gtactcgctc agctccgtgc gctagcttta ggcaatgagc gtattaccgc tggtttattg
       61 cggcaagtgt atcaagatga gttaaagcct gtgcacccca tgctagaggc attacgctcg
      121 ggtatcccag aacgcattgc tcgttattct gatctagtcg ttcccgagat tgataaacgg
      181 ttaatccaac ttcagctaga tatcgcagcg atacaagaac aaacaccaga agaaaaagcc
      241 cttcaagagt tagataccga agatcagcgt catttatatc tgatgctgaa agaggattac
      301 gattcaagcc tgttaattcc cactattaaa aaagcgttta gccagaatcc aacgatgaca
      361 agacaaaagt tactgcctct tgttttgcag tggttgatgg aaggcgaaac ggtagtgtca
      421 gaactagaaa agccctccaa gagtaaaaag gtttcggcta taaaggtagt caagcccagc
      481 gactgggata gcttgcctga tacggattta cgttatatct attcacaacg ccaacctgaa
      541 aaaaccatgc atgaacggtt aaaagggaaa ggggtaatag tggatatggc gagcttattt
      601 aaacaagcag gttagccatg agaaactttc ctgttccgta ctcgaatgag ctgatttata
      661 gcactattgc acgggcaggc gtttatcaag ggattgttag tcctaagcag ctgttggatg
      721 aggtgtatgg caaccgcaag gtggtcgcta ccttaggtct gccctcgcat ttaggtgtga
      781 tagcaagaca tctacatcaa acaggacgtt acgctgttca gcagcttatt tatgagcata
      841 ccttattccc tttatatgct ccgtttgtag gcaaggagcg ccgagacgaa gctattcggt
      901 taatggagta ccaagcgcaa ggtgcggtgc atttaatgct aggagtcgct gcttctagag
      961 ttaagagcga taaccgcttt agatactgcc ctgattgcgt tgctcttcag ctaaataggt
     1021 atggggaagc cttttggcaa cgagattggt atttgcccgc tttgccatat tgtccaaaac
     1081 acggtgcttt agtcttcttt gatagagctg tagatgatca ccgacatcaa ttttgggctt
     1141 tgggtcatac tgagctgctt tcagactacc ccaaagactc cctatctcaa ttaacagcac
     1201 tagctgctta tatagcccct ctgttagatg ctccacgagc gcaagagctt tccccaagcc
     1261 ttgagcagtg gacgctgttt tatcagcgct tagcgcagga tctagggcta accaaaagca
     1321 agcacattcg tcatgacttg gtggcggaga gagtgaggca gacttttagt gatgaggcac
     1381 tagagaaact ggatttaaag ttggcagaga acaaggacac gtgttggctg aaaagtatat
     1441 tccgtaagca tagaaaagcc tttagttatt tacagcatag tattgtgtgg caagccttat
     1501 tgccaaaact aacggttata gaagcgctac agcaggcaag tgctcttact gagcactcta
     1561 taacgacaag acctgttagc cagtctgtgc aacctaactc tgaagattta tctgttaagc
     1621 ataaagactg gcagcaacta gtgcataaat accaaggaat taaggcggca agacagtctt
     1681 tagagggtgg ggtgctatac gcttggcttt accgacatga cagggattgg ctagttcact
     1741 ggaatcaaca gcatcaacaa gagcgtctgg cacccgcccc tagagttgat tggaaccaaa
     1801 gagatcgaat tgctgtacga caactattaa gaatcataaa gcgtctagat agtagccttg
     1861 atcacccaag agcgacatcg agctggctgt taaagcaaac tcctaacgga acctctcttg
     1921 caaaaaatct acagaaactg cctttggtag cgctttgctt aaagcgttac tcagagagtg
     1981 tggaagatta tcaaattaga cggattagcc aagcttttat taagcttaaa caggaagatg
     2041 ttgagcttag gcgctggcga ttattaagaa gtgcaacgtt atctaaagag cggataactg
     2101 aggaagcaca aagattcttg gaaatggttt atggggaaga gtgagtggtt aggctagcta
     2161 catttaatga caatgtgcag gttgtacata ttggtcattt attccgtaac tcgggtcata
     2221 aggagtggcg tatttttgtt tggtttaatc caatgcaaga acggaaatgg actcgattta
     2281 ctcatttgcc tttattaagt cgagctaagg tggttaacag tacaacaaag caaataaata
     2341 aggcggatcg tgtgattgag tttgaagcat cggatcttca acgagccaaa ataatcgatt
     2401 ttcctaatct ctcgtccttt gcttccgtac gcaacaagga tggagcgcag agttcattta
     2461 tttacgaagc tgaaacacca tatagcaaga ctcgttatca catcccacag ttagagctag
     2521 ctcggtcatt atttttaatt aactcctatt tctgtcgaag ctgtttgagc agtaccgctt
     2581 tacagcaaga gttcgacgtt cagtatgagg ttgagcgaga tcatttagag ataaggatct
     2641 tacccagttc atcgtttcct aaaggggcgt tagagctgtc ggccgtagtg cagcttttgg
     2701 tttggttgtt ttcggatcaa gatgttatgg attcgtatga aagtattttt aggcactatc
     2761 aacaaaatag agaaattaag aacggcgttg aaagctggtg ctttagcttt gaccctccgc
     2821 ccatgcaggg ttggaaatta catgtaaaag gacgttcttc taacgaggat aaggattatt
     2881 tagttgagga aatagtaggt ttagaaatca acgctatgct tcctagcaca acagctatta
     2941 gccatgcctc ttttcaggaa aaggaggcag gtgatggtag tacgcagcac atagcggttt
     3001 caacagagtc agttgttgat gatgagcatc tacagttgga cgatgaggaa acagccaata
     3061 tagacacaga cacacgagtc atagaggctg agccgacatg gataagtttt agtagaccta
     3121 gtcgaattga aaaatctcgc agggcaagaa aaagtagcca aactatttta gaaaaagaag
     3181 aagcaacaac aagtgaaaat agtaatttgg ttagtactga tgagccacac ttaggtggtg
     3241 tcctagcagc ggcagatgtg ggtgggaagc aggatgcaac caattacaac tctatttttg
     3301 ctaatcgatt tgctgctttt gatgagctac tttcaattct aaaaactaaa tttgcatgtc
     3361 gggtgctttt tgaagaaacc ttggttttgc caaaagttgg gcgtagccga ttacatctgt
     3421 gtaaagatgg ctcaccaaga gtgattaaag ccgttggggt gcaacgtaat ggcagtgaat
     3481 ttgtattgct agaggtggat gcatcggatg gggtgaaaat gctttctacc aaagtgttga
     3541 gtggcgttga tagcgaaaca tggcggaatg attttgaaaa gatacggcgt ggagtggtga
     3601 agagctcatt gaattggcca aatagtttgt ttgatcaatt atatggacaa gacgggcata
     3661 gaggggtgaa tcatccaaag gggttggggg agctgcaagt atcgagagag gatatggaag
     3721 ggtgggctga gagagtggtt agagagcaat ttacgcatta aaggaatgac tgaaagagcc
     3781 tgtaaaccct tttgtgtaag tgcttttgcc ggtcagttaa aggtggccat ttaaacggtc
     3841 accaaattcg atcataaaac ggttcatggc cggcttccag ttgcggatcg gcatcgtcca
     3901 tttcttggtc gccgcctgga tagccaggta caccaccttc atcgctgatt cgtccgtagg
     3961 gaacaccttg agttcatatt aatggaattt tctacaaata gcctccgtgg ttttgagggg
     4021 ggattacaga cgatccatag tagtaatcca atgagttctt gagcgcggcg acatgtttgg
     4081 acgccttggc aaaaattaga gcctgcttga agtgcaggcg agcacgtgct tggacgattg
     4141 atccattcgc ggtcaaaaac tcaatcttgg atgacagcgt gtcaggaaat ccaatatcga
     4201 agtcccgccc cgtgtcaaag tagggcattt catgatcaaa ggacgaagct tcccaggcca
     4261 cttccctaat attccagccg ctagaaacaa gatttcgtac gacttcatca acttttttgc
     4321 tttccaactt gtcacaaagt gcataggcaa gagctttggc ggcagacacg tgcgtttcct
     4381 cttaaacata ggtcgaacgg atacccgagg caaagcggag cattagtccc aaagcatctc
     4441 cggtagatcc ggaggctcca tactgaaggc atggcatctt gggcattgat atttgaaact
     4501 tctctctaga tagcagggat ttcttggcct tgcctcatgc tgatagcttg taaatatcac
     4561 acgatcctta gccccacaaa gtgagcatac tccgtagctt tcatttatag aaatcgtcgt
     4621 tagattgtcg cagcatgtgc acgttccgac tatttgctca ggaagcatc
//
LOCUS       DAZPDE010000231         4603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPDE010000231 DAZPDE010000000
VERSION     DAZPDE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.235"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHU0J_003764"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACHU0J_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1218241.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHU0J_003765"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACHU0J_003765"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1218242.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHU0J_003766"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACHU0J_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1218243.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHU0J_003767"
     CDS             complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACHU0J_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1218244.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAZPDE010000232         4564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPDE010000232 DAZPDE010000000
VERSION     DAZPDE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.236"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            23..751
                     /gene="gadW"
                     /locus_tag="ACHU0J_003768"
     CDS             23..751
                     /gene="gadW"
                     /locus_tag="ACHU0J_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1218245.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1114..4227)
                     /gene="mdtF"
                     /locus_tag="ACHU0J_003769"
                     /pseudo
     CDS             complement(1114..4227)
                     /gene="mdtF"
                     /locus_tag="ACHU0J_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(4252..4509)
                     /locus_tag="ACHU0J_003770"
                     /pseudo
     CDS             complement(4252..4509)
                     /locus_tag="ACHU0J_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1141 a         1155 c         1140 g         1128 t
ORIGIN      
        1 gtatgaaata taagggataa tcatgactca tgtctgctcg gtgatcctca ttcgtcgttc
       61 attcgatatt tatcatgaac agcaaaaaat atcgctgcat aacgagagta tcctgctgct
      121 ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg gatacgcgac gactggatat
      181 cgatgagctg acagtttgcc attacttaca aaatattcgt cagctaccac gcaatttagg
      241 gttacatagc aaagaccgtc tgttaattaa ccagtcaccc cccatgccgc tggtgacggc
      301 gatttttgat agcttcaatg aatccggggt aaattcaccg atactgagca atatgctcta
      361 cctttcctgt ttatcgatgt tttctcataa gaaagaactg atccccttac ttttcaatag
      421 catcagcact gtttcaggaa aagttgaacg ccttattagc tttgatatcg ccaaacgttg
      481 gtatctgcgc gatatcgcgg aaagaatgta taccagcgag agtctcatca aaaaaaagtt
      541 gcaggatgaa aatacctgtt tcagtaaaat attactcgct tccaggatgt cgatggccag
      601 acgattactc gagttacgtc aaattcctct gcatactatt gcggaaaaat gtggctatag
      661 cagtacgtcg tactttataa acacatttcg acaatattat ggtgtaacgc cacatcagtt
      721 tgcgcaacat tcgccaggta ccttttcctg acatattttg catttgaata ttggtcagga
      781 tctcacacct gcttcatgtg aaactcttcc ctgatgattt ctgccgggct accggctagt
      841 tctctttcgc agtttgccag gcggtatagg tatttttagg aggaaaagac tcggcatgtt
      901 tgggattatt aagctgacaa ttcatacgat tacgggaatg tgggtcagta ttgtgttgtt
      961 caaattgatg acgaatggct ggagtggttt ctactttcag tgctgtgtac taagcctggt
     1021 attcctgacg gttagctggt tgttgagtgg tgaatggtta gcaggaaaga gtaaggctga
     1081 accttcacgt tcaaccttac tctcatttac acgttacgct tttttaaagc gggcaaagag
     1141 atgttccact acaacgaaaa agaccggaac gaagtaaatt gccagcactg ttgcggcaaa
     1201 catcccgccc atcacgccgg tacctaccgc gttttgcgcg ccagaaccgg caccatgact
     1261 gataaccagc ggcagcacgc cgagaataaa ggccagagag gtcatcagga ttgggcgtaa
     1321 acgcatccgc gccgcttcga tgattgcctc tatcggcgtt ttcccttctt tctgcatcat
     1381 ctcaacggca aattcgacaa tcaggatggc gttcttggcg gaaagcccga tggtggtcag
     1441 caaaccaacc tggaagtaga cgtcattact taagccgcgc agatcggtgg ccagtaatgc
     1501 gccaacgacg cctaacggaa caaccaacat caccgagaac ggaattgacc agctctcata
     1561 gagtgcggcg agggcgagga acaccacgac cagtgaaatg gcatacagtg caggagcctg
     1621 gtttgaggat aacgcttcct gatacgacag tccggtccat gagtagccga cgcctgccgg
     1681 aagtttagcg accaggtctg ccataaattt catagcgtca ccggtacttt tcccggccgc
     1741 cgcttcacct aaaatctcca ttgacgggat gccgttgtag cgttccaatc gcggtgaacc
     1801 ataggtccat tcggtagacg agtaggcaga aagcggtgcc atcgtgccag aggcgttgcg
     1861 tacataccat tggttgatgt tatccggcaa catacggaac ggcgtgcctg cctggacata
     1921 cacttttttc acccgcccct ggttgaggaa gtcgttcacg tagctgctgc cgaaggcggt
     1981 ggaaattgtc tggttgatat cagacagcgc cacacccatt gcctcagctt tcgcggcgtt
     2041 gacgttcact ttgaacatcg gcgtatcttc caggccgttc gggcgtaccc cggtgacctg
     2101 gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt gcctaggtta gtttttcgtg
     2161 ccccaggtta ccgttgtcca gcagttccat atcaaaacct gacgcggtac ccagttcagc
     2221 caccgcgggt aagttgaacg ggaagacgac ggctttattg atactgctta acgcaatcat
     2281 tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg acacgttcag accacggctt
     2341 gagactgata aacgccaggc cgttgttttg cccctggccg ctgaagccaa agccgccaac
     2401 ggtaaacacc gactgaacat tatctttctc ttttgtaaga taataatccg tcacctgttg
     2461 cagtactttc gtggtgttaa ccatggtggc accggaaggt aactgcgcgg tggtcataaa
     2521 taccccctga tcctcttctg gtaagaaaga ggtcggtgta cgcaggaaca gcaccgccat
     2581 cccggcgcaa atcagcaggt agaccaccat gtagcgaccg gtacaacgca acagcgagcg
     2641 ggtgctatcg gtatagtgct gagttgattt ttcaaacagc gtgttgaagc gtgcgaacag
     2701 ggcgttaggt ttgtgaccgc cttccggcgt ggctttcaga atggtggcgc acagggcagg
     2761 ggtcaggctc attgccacaa atactgaaag ccgcatggag gagatcagcg tgatggagaa
     2821 ctggcggtag atctccccgg ttgcaccgct cataaaggcc atcggcataa acactgcaga
     2881 aagaacaacg gcaatcccga ccagcgcacg ttggatctgc cccatcgatt tatgcgtcgc
     2941 ttccttcggc ggtagcttat cttccgcaat gacacgctcg acgttctcca ccaccacgat
     3001 ggcgtcatcc accagtaacc ctatcgccag caccatcccg aacatagtca acgtgttgat
     3061 ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga ataaccaccg gtacggcaat
     3121 tgtcgggatg atggtggcac ggaaattctg caaaaacaga taaatgacca ggaagacgag
     3181 gatgatagcc tcaaccagtg ttttgaaaac ttcctgaata gaaatttcga taaacggcgt
     3241 ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa taggctgata agcggttcag
     3301 ctcctctttg actgcccgcg aggtatccag ggcgtttgct ccggcagcca gtttgatggc
     3361 gatcccggca acaggtttgc cgttatagcg cgccacggtg gaataatctt ccgccccaag
     3421 ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca tcttgctgaa ctttcaacag
     3481 gattttgcca aattcttccg gcgtttgcag acgcgtctgc acaatgatcg aggcgtttag
     3541 ttgctggtct gccgcctgtg gcatgccacc cagttgacca ccagaaatct ggttgttttg
     3601 caccttaatc tgggaaataa catcggaagg taccaggtta tatttattga gtttttgcgg
     3661 gtccagccag atacgcatgg cgtactcgga accaaagagc tgtacgctac caacgcccgc
     3721 ggtacggctt agcgggtctt tgatattaga cgctacatag tccgcgatat cgtactggtt
     3781 gaggctgccg ttatcagaaa taaacgccgc taccatcagg atattactgc tcgacttatc
     3841 gacgctaatc ccctgttgct gcaccgcttc aggtaatgaa ggcatagcga gttgcagttt
     3901 attttgcacc tgaacctgtg cgatatcagg agatgcccca gtctcgaagg tcagagtgat
     3961 agaggcatta cccgccgcat cactggttga agacatgtac atcaggccat caagcccatt
     4021 catattttgc tcaatcacct gagtgaccga gtcttctacc gtttgcgcat cggcacccgg
     4081 ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc ggatactgcg caaccggtaa
     4141 gttcatgatc gccagaccac ctgcaagcat cataataatg gcaagtaccc aggcaaaaac
     4201 cgggcgatca ataaaatagt tagccatgaa agtcccctta agcctgcaac gttattgttt
     4261 cgattcggtg ctggcgtttt cctggctgga ggaaattgct cgtgctttga tacccggacg
     4321 aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc tgcaagccag aggtgacaac
     4381 ccactggtcg ccgatggctt tgctggcttc aatttcgcgt agctgcacga catcgtcttt
     4441 atccagaatg agcgccgttg ctttaccctg ggcgttgtgg gtgacgcctt cctgcggcac
     4501 cagtaatata aagcgcgcaa aattattcaa gatatttttg aaaatcattc aagataggct
     4561 atcg
//
LOCUS       DAZPDE010000233         4352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPDE010000233 DAZPDE010000000
VERSION     DAZPDE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.237"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..194)
                     /locus_tag="ACHU0J_003771"
     CDS             complement(<1..194)
                     /locus_tag="ACHU0J_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218246.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
     gene            complement(251..2611)
                     /locus_tag="ACHU0J_003772"
                     /pseudo
     CDS             complement(251..2611)
                     /locus_tag="ACHU0J_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(2649..4334)
                     /locus_tag="ACHU0J_003773"
                     /pseudo
     CDS             complement(2649..4334)
                     /locus_tag="ACHU0J_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1184 a          968 c          967 g         1233 t
ORIGIN      
        1 aatgaaccgg tatgaatttg cagccataaa tttacctgat cctttggatg agcatgcgga
       61 taaatcatat atcgcaagtc attgtccagt tgcccggtaa ttaacttttc atcctgcggt
      121 aaggcggcgg caatcagccg cccggggagc aacagagttg ccactaacgt cagtaagaaa
      181 cagaggtttc tcataattat ctccatgcga aaaccgggcg aatttacccg gttaagtaaa
      241 atccgaacta ttaaaatttc atgctgacat caagccagaa agtacggcca ctggcgtagg
      301 tcgccacacc ggtgttcgtt gtatcaacag cggttttgct atccagtaca ttgagaatat
      361 cggcactgat cgtcaggttt tgttgtttca gaaattgtgg cgtccaggac aagcgggtgt
      421 cccaggtcag gctgctatcc agtttttcgt caacatactg cttgtaatct gaatattcgc
      481 tgatgtattg cgcatttgtc ttaccaagga taatgcgagc tttacgcgct tcttgccagg
      541 ccagcgtatt cgcccacact aacccgctcg gttgatgcgt gaaatccatg tttaaggaga
      601 tctttaatgg gttattaaaa tctgccactg gaacgctatc gtaagagacc agattaccgt
      661 tataaaccac ctggttatca ccggtattgc tctcctcata accattgttt aacgacaagt
      721 tgcccttgct cttgatatag ctaaagacaa tttgcgggtt aatatctacc tggcggatat
      781 gcaggggttc ggccagttca aaactgaggt taaacgaatg cgttttggtt ttgccatcgt
      841 tgttatattc agtaatggtg gttttagtcg cgctgtcggt acgactgctt ttgctgattt
      901 gatcatgcgc ttcacttgcg cgtgcaataa cgttcttatc gattttttgc tgcaatccca
      961 ttgccagttc atcgttataa ggcgttttca aattctgata acgcgtcagg gttttattac
     1021 ctgataccga ttccgtccag ctattgcgga tatcacgtaa tcccatatca agaatattcc
     1081 cgccatagta acggttataa ccggcggtaa tcattgaggt ttgatcagca aaaatatccc
     1141 attccgtcat aaagcgcggg gagatattgt ggtttgacag atagttgtca tagtcataac
     1201 gcacaccggg cattaacgac acattccgcc agctaatgtg atccgccata tacagcgtgt
     1261 agttgtcaat tcccaggctg cctttacctt tatggtaaat ggtatggtta gttttctttc
     1321 ctgcagcatt aatcacatag gattcagact ggttatggcg ttcagtccat gcatcggaat
     1381 agatgtattc cgcgccgaag tagggttgat gcgaaacatc acccacggcg aatttttgcc
     1441 agtccaggcg tgttttgaag gtgtaattat ctacagcctg ggaaatgtgt cctaatccgc
     1501 cacgggtaca acgccctgta atatcaccat atgtacatga aagttcggtg taccagatgt
     1561 catgatcgtg acgggtataa tcactaatat gatcccaacc aacggtggta cgtagtttgg
     1621 cccatgcgag ttgcgtatcc atatcctatg ccagaccata ggatttatta cccatttcgc
     1681 gatcagactg cgggaaggtg ctggtattat aatcacggct ggagccggta tattttaagg
     1741 ttaaatcgtg ggtaaagcgg tcgctggcaa accaggtaaa tttgctcaat gcagtatcga
     1801 taacgttttt atactgtgcc cgaccggcga caatgccgtc attcgaaaca taatccgcgc
     1861 gggtgatatc agactggcgg cgcgataaac cggcggtaac gccaaagtta tcagccagct
     1921 cctgattaaa cgacaaggta taaaagttct ttttaaaatc tggagagaaa taggtacttc
     1981 ctgaagaacc ttgattaaat gcgctcttgt tattctcatc gatatgcgat gttaaccagt
     2041 ccaaacgcgt agtgcgataa cccaatttca ccttgctatc atcagcgttg aagcgtttga
     2101 tctttgcatc aattaccccg ccattgaagc gaccaaattc aaccggcaca aaactgtcat
     2161 aaagcgtcac attgtccagt aagctgacat caagataata cccctgtgac atcccgctaa
     2221 tattggttgc actactggca tcggactcat tcgctgggtt caggttatta gttgcactaa
     2281 taccgtcaat caaataggca ttctggtagg gcgacgcacc gtgaatagag attttctcag
     2341 ggcgaatatc tccctggttc aacgaggtac tttgcgttga atccatgcgt accgcagggt
     2401 tggttctcag cagatcgctg atattgccat tcccggtcgg taaacgctcg atgctttcac
     2461 tggtgtaatg cgtgttgccg tttacgggta cagggacagg agcatagacc gtcatctctt
     2521 caaaaaaata catgtacatg tttttgtcat cgacggcctg cgccacatca gcgccacata
     2581 aaatgacgcc aggaataaga actcgcttca tttatttata tctccgtaca gcagggcttt
     2641 tattgttgtt ataaaaccgt gctaatgtca caaatatcat cagccaggtt ccagacaccg
     2701 ggttgatgtg taaccataat gacgccgctt gtgggtagtt tttcacgcac taaacgcagt
     2761 aagcgtatag cctcttgttc ctcaagatga gaggtagttt cgtcaagaaa tatccatttc
     2821 ggacgtcgta aaattaatcg tgccagggcg atacgttgtt tttcgccgct ggaaagaata
     2881 tctccccagc gatcgtggtc atgaatacgc gcagccaatt ttcccagacc aacctgatgc
     2941 agtacttcgc tcaacgattt atcgtctacg ggcaggggaa gtgctttaca aataatctct
     3001 ttcagtaagc cgcttttgat taacggtgtt tgtgacacat accaactgtc agcaggagaa
     3061 gaaatatcac ctttaaacca cggccagcag tgggataatg ttttaagcag tgtggttttt
     3121 cccgcaccag agtagccttt cagtaatagc catttgcctg gcgaaacatg aaagttcaag
     3181 ttctctaata tgatcttatt atcaggcgta cgaatactcg cattagccac ttgtaccgca
     3241 tgttggcaat ttttaggctt attcgtaggg cgctattcag tgagttgatg gaactcatac
     3301 aagcgatcga taaccgcagc cagttcagca agttcgtcat atttataaat aaaccagctt
     3361 aaattgttcg atactagcat aaatgcctgg cgcgatttca tcagtccgcc cagattgatc
     3421 tgcccgctaa taaactgcgg caataacaga aagtagggaa gaacgctaag cgagcgcgaa
     3481 taaatatttt gccagtaatc aagccaccgc tgacgattca ttaaacgatg ccagttctct
     3541 ttaatggtat gaaaattgtc gcgcaactcc tggcgttgta gagactcagc attgcttagc
     3601 gcaatcagtt ctgcctgctt attatgctgc acaagattag ttcgaaatgt cgcttcgcta
     3661 cgttgttttt ccacattaag cggacgaata cgtttaccca ccttatgggt aaataaagtt
     3721 ccaccgatca caatgagcac gacggtatag accatatacc cctggatatt ccattctgtt
     3781 ccaccaacag tgaatgagag cgtacccgcg ctttgccaaa gaataacggt aaaggtgatc
     3841 agcatgctaa gtgactggat gaagccaaat gaaagactga gcgttttgtt gatcagtaag
     3901 agaatgtctt cagcgatacg ttggtcaggg ttatctgtat ttttatgctc gccgtagatc
     3961 tgcgtgaagt agtagttttt atctgcaaac cagcgattaa ggtagtaatc tgttaaccat
     4021 tcgcgccagc gaatagtcag tagtttaatt aaccatgttt tgtttgcgga tattaataca
     4081 aagatcccca gcagggcagg gaaccaaaga acgagctgcc aaagcttatc ggtttctttc
     4141 tggcttagcg cattgaaaaa atcattattc cagtcgttta accagacctg aattttaacc
     4201 acgccaagga tcattgcgag gataataatg attaacaaca ctgaagtttt attattctta
     4261 cgcagccaaa agggcttcag cagacagaga tactttgcta tcaacataca aaacgtaatg
     4321 ggaatggtta tcattagcga aaattgattg tg
//
LOCUS       DAZPDE010000234         4321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPDE010000234 DAZPDE010000000
VERSION     DAZPDE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.238"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            436..3744
                     /gene="icsA"
                     /locus_tag="ACHU0J_003774"
                     /gene_synonym="virG"
     CDS             436..3744
                     /gene="icsA"
                     /locus_tag="ACHU0J_003774"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR1218247.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3888..4206
                     /locus_tag="ACHU0J_003775"
                     /pseudo
     CDS             3888..4206
                     /locus_tag="ACHU0J_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1313 a          740 c         1019 g         1249 t
ORIGIN      
        1 taagaaaacg atacatttcc actaaaaaca attatgcagt tattgcattg gagagtctgg
       61 taggaaagtt gtgatatgaa tacattcatg ttattggctt tggggattaa catcaagggg
      121 cagatagaac cattgggtgt atagaaaaac aaagtggtta aactttgggc aagcgtacag
      181 aaagaactga aaagttgcgg tctgaagcag actatcatag taaaattatt aatttattat
      241 cgaacatata gctttccccc tctttttttc aaagcaagac acaggtaaat ttctcccgtt
      301 gcattgatat ataacacagc tctcatgttt tggttgaggc tttgtttaat atgtttttgc
      361 atatatcgtc cctttattcc ggataaaacg gaatcttttc aggggtttat caaccactta
      421 ctgataatat agtgcatgaa tcaaattcac aaattttttt gtaatatgac ccaatgttca
      481 cagggggggg ccggagaatt acctacggta aaggaaaaaa catgcaaatt gtctttttct
      541 ccttttgttg ttggtgcatc cctgttgctc ggggggccaa tagcttttgc tactcctctt
      601 tcgggtactc aagaacttca tttttcagag gacaattatg aaaaattatt aacacctgtt
      661 gatggacttt ctcccttggg agctggtgaa gatggaatgg atgcgtggta tataacttct
      721 tccaacccct ctcatgcatc tagaactaag ctacggatta actctgatat tatgattagc
      781 gcaggtcatg gtggtgctgg tgataataat gatggtaata gttgtggcgg taatggtggt
      841 gactctatta ccggatctga cttgtctata atcaatcaag gcatgattct tggtggtagc
      901 ggcggtagcg gtgctgacca taacggtgat ggtggtgagg ctgttacagg agacaatctg
      961 tttataataa atggagaaat tatttcaggt ggacatggtg gcgatagtta tagtgatagt
     1021 gatgggggga atggaggtga tgccgtcaca ggagtcaatt tacccataat caacaaaggg
     1081 actatttccg gtggtaatgg aggtaacaat tatggtgagg gtgatggcgg taatggaggt
     1141 gatgccatca caggaagcag cctctctgta atcaataagg gcacgttcgc tggaggcaac
     1201 ggaggtgctg cttacggtta tggttatgat ggctacggtg gtaatgctat cacaggagat
     1261 aacctgtctg taatcaacaa tggagctatt ttaggcggta atggtggaca ttggggggat
     1321 gctataaatg gtagcaatat gaccattgct aatagcggat atataatttc aggtaaagaa
     1381 gatgatggaa cacaaaatgt agcaggtaat gctatccaca tcactggtgg aaacaattca
     1441 ttaatactcc atgaaggttc tgtcattact ggtgatgtac aggttaacaa ttcatccatt
     1501 ctgaaaatta tcaacaatga ttacactggg accacaccaa ctattgaagg tgatttatgt
     1561 gctggtgatt gtacaactgt ttcactatca ggtaacaaat tcactgtttc aggtgacgtt
     1621 tcttttggtg agaacagttc tttaaattta gctggaatca gtagtctgga agcttctgga
     1681 aatatgtcat ttggcaacaa tgtaaaagtg gaggctatta taaataactg ggcgcagaag
     1741 gactataaac tgctaagtgc agataaaggg ataacaggtt tcagtgtttc taatatatct
     1801 atcatcaatc cgttactcac tactggtgct attgactata caaaaagcta tatcagtgac
     1861 cagaataaat tgatctacgg tttgagctgg aatgatacag atggcgacag tcatggagag
     1921 ttcaatctga aagaaaacgc tgaacttact gttagtacta ttctggcaga taatctcagc
     1981 catcataata taaatagctg ggacggaaaa tccctaacaa aatcagggga gggaactctc
     2041 attttggcgg aaaaaaatac ctactctggt ttcaccaaca tcaatgcagg cattctaaaa
     2101 atggggacag ttgaagctat gacacgtacc gctggtgtta ttgttaataa aggtgctacc
     2161 ttgaattttt caggcatgaa ccaaactgtt aacactttat taaatagtgg gactgtgcta
     2221 atcaataata ttaatgcccc ttttttgcct gaccccgtca ttgtcacagg taacatgact
     2281 ctggagaaaa acggtcatgt tattctcaat aatagttcgt caaatgtcgg tcagacctat
     2341 gttcagaaag gtaattggca tggaaagggc ggaatattat ctttgggcgc ggttctcggc
     2401 aatgacaaca gtaaaactga ccggctggaa attgcaggcc atgcgtctgg tattacctat
     2461 gttgcagtga caaatgaggg aggctctgga gataaaactc ttgaaggtgt tcaaattatt
     2521 tcgacagatt cttctgataa gaatgctttt attcagaaag gccgtattgt tgctggtagt
     2581 tatgactatc gcctgaaaca gggcactgta tctggactga ataccaataa gtggtatcta
     2641 actagtcaga tggataatca agaatcaaaa cagatgagca atcaagagtc tactcaaatg
     2701 agtagtcgcc gagctagttc acagcttgta tcttcactta atttgggtga aggtagtatt
     2761 cacacatggc gccctgaagc tggcagttat attgctaacc tgatagcaat gaacacgatg
     2821 tttagtcctt ctctctatga ccgacacggt agcactattg ttgatcctac tacaggtcag
     2881 ctcagcgaaa ccaccatgtg gattcgtact gttggtggac ataatgagca taatttagct
     2941 gatagacaat taaaaaccac agctaacagg atggtttatc agattggtgg agatattttg
     3001 aagacaaact tcactgatca tgatggcttg catgtgggta ttatgggagc ttatggatat
     3061 caggatagca aaactcataa taagtatact agttatagtt cacgaggaac tgtgagcggt
     3121 tatactgccg gtttgtacag ttcttggttt caggatgaaa aagaacgaac aggtctatat
     3181 atggatgctt ggttgcagta cagttggttt aataatacag tcaaaggaga tgggttaact
     3241 ggtgagaaat attccagcaa aggaataaca ggagctttgg aagctggcta tatctaccca
     3301 accatacgct ggactgctca taataatatt gacaacgcat tgtatctcaa tccacaagtc
     3361 cagataacta ggcatggggt aaaagcaaac gactatattg aacacaatgg cactatggtc
     3421 acatcctctg ggggcaataa tattcaagca aaattgggat tgcgtacatc cttaattagt
     3481 cagagttgta tcgataagga gactcttcgt aagttcgaac catttttgga agtgaattgg
     3541 aaatggagct caaagcaata tggtgtaatt atgaatggca tgtcaaatca ccagataggc
     3601 aaccgtaatg tgattgaact caaaactggt gtgggggggc gtcttgcaga taacctaagc
     3661 atctggggaa acgtatctca gcaattgggt aataacagtt acagagacac ccaaggtatt
     3721 ttgggtgtga aatatacctt ctgataataa aaatgcaggg cgtgttgatg tcctgcattt
     3781 ctctgggacc caccgtgtaa aagctcggtg ggtttttgtg cacatgatgg tatgcctgcg
     3841 atcaatgcca aacgtgttta ccagatcatg cgccagaatg cgctgttgct tggcgttaca
     3901 caggaattgc attagaaagc gatgaacgaa tgctaccgcc aaatattcgt ggttatgcgc
     3961 ctcagattac tggtattgca gaaactaatg cacgtgttgt tgtatctcaa caaggtcgag
     4021 taatatatga ctctactgta ccagctggaa ctttttctat tcaggacttg agtagttctg
     4081 tacgaggaat attggatgtt gaaatttttg agcaaaatgg taaaagaaaa catttccaag
     4141 tagaaatgtg caggtgtgcc tttcttatcc agacttggtc agagtaggta taaattagta
     4201 acaggtctac ctaaaacaaa taataaagca actggtgatg catttttttc ggtgaagatc
     4261 ctacggggtc ctgagcccgg tgaagtggcg aggcaaatca catggggaag cgtacagcct
     4321 g
//
LOCUS       DAZPDE010000235         4304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPDE010000235 DAZPDE010000000
VERSION     DAZPDE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.239"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(189..935)
                     /gene="cutC"
                     /locus_tag="ACHU0J_003776"
     CDS             complement(189..935)
                     /gene="cutC"
                     /locus_tag="ACHU0J_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1218248.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(949..1515)
                     /locus_tag="ACHU0J_003777"
     CDS             complement(949..1515)
                     /locus_tag="ACHU0J_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1218249.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1731..3464
                     /gene="argS"
                     /locus_tag="ACHU0J_003778"
     CDS             1731..3464
                     /gene="argS"
                     /locus_tag="ACHU0J_003778"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1218250.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3506..3550)
                     /locus_tag="ACHU0J_003779"
     CDS             complement(3506..3550)
                     /locus_tag="ACHU0J_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218251.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3641..4129
                     /locus_tag="ACHU0J_003780"
     CDS             3641..4129
                     /locus_tag="ACHU0J_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1218252.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1141 a         1065 c         1043 g         1055 t
ORIGIN      
        1 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
       61 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
      121 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      181 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      241 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
      301 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
      361 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
      421 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
      481 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
      541 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
      601 gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc gagaacgccc
      661 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
      721 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
      781 atcgtcaccc gctggcgcac ggatttcagt acccccaacg acggcgttaa gcccccctct
      841 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
      901 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
      961 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     1021 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     1081 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     1141 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     1201 acgtttttcc ccgggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     1261 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     1321 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     1381 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     1441 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     1501 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     1561 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     1621 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     1681 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     1741 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     1801 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     1861 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     1921 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     1981 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     2041 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
     2101 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac acagcagtgc
     2161 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
     2221 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
     2281 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
     2341 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
     2401 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacttacg
     2461 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
     2521 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
     2581 cgaccgtcgt attccttgat gtgtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
     2641 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
     2701 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
     2761 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
     2821 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
     2881 cgggtggtac cgtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
     2941 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
     3001 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
     3061 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
     3121 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
     3181 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
     3241 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
     3301 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
     3361 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
     3421 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
     3481 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
     3541 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
     3601 cccaggaaaa cgataacggt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
     3661 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
     3721 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
     3781 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
     3841 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
     3901 tactgaacaa cttaacttgc ttaaaaaatc acagcgagcc tggctggatt ttagggacaa
     3961 agaatgtgaa ttaatccttt caaatgagga tgttcaggat ttaagtgacc cctattctga
     4021 atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
     4081 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatcttt
     4141 ttggcatgtt ttgttgcaag ctattcctga taaatgattg caacaaaaca tccagccttt
     4201 tgactgagtt attaaacata ctcgcgagcg cgtaattttt tgtctttcag cgataattca
     4261 caatcacttc attccgttgt acctttagcg gtggaattaa ccgc
//
LOCUS       DAZPDE010000236         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPDE010000236 DAZPDE010000000
VERSION     DAZPDE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.242"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHU0J_003781"
     CDS             complement(<1..209)
                     /locus_tag="ACHU0J_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1218253.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHU0J_003782"
     tRNA            complement(324..399)
                     /locus_tag="ACHU0J_003782"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHU0J_003783"
     tRNA            complement(406..480)
                     /locus_tag="ACHU0J_003783"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHU0J_003784"
     tRNA            complement(597..681)
                     /locus_tag="ACHU0J_003784"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHU0J_003785"
     tRNA            complement(690..765)
                     /locus_tag="ACHU0J_003785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHU0J_003786"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHU0J_003786"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1218254.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHU0J_003787"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHU0J_003787"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1218255.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHU0J_003788"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHU0J_003788"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1218256.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZPDE010000237         4198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPDE010000237 DAZPDE010000000
VERSION     DAZPDE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.243"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHU0J_003789"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHU0J_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1218257.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHU0J_003790"
     CDS             complement(995..1690)
                     /locus_tag="ACHU0J_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1218258.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHU0J_003791"
     CDS             complement(1683..3110)
                     /locus_tag="ACHU0J_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1218259.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHU0J_003792"
     CDS             complement(3121..3840)
                     /locus_tag="ACHU0J_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1218260.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZPDE010000238         4185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPDE010000238 DAZPDE010000000
VERSION     DAZPDE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.244"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHU0J_003793"
     CDS             <1..192
                     /locus_tag="ACHU0J_003793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218261.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHU0J_003794"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHU0J_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1218262.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHU0J_003795"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHU0J_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR1218263.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHU0J_003796"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHU0J_003796"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1218264.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          960 g         1035 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAZPDE010000239         4184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPDE010000239 DAZPDE010000000
VERSION     DAZPDE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.245"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHU0J_003797"
     CDS             complement(<1..188)
                     /locus_tag="ACHU0J_003797"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1218265.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="ACHU0J_003798"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="ACHU0J_003798"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1218266.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="ACHU0J_003799"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="ACHU0J_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1218267.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="ACHU0J_003800"
     CDS             3865..4146
                     /locus_tag="ACHU0J_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1218268.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1045 c         1062 g         1061 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatcccta taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac aggcttcagg taagtcatca
      901 gtacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgggcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaa
//
LOCUS       DAZPDE010000240         4168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPDE010000240 DAZPDE010000000
VERSION     DAZPDE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.246"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHU0J_003801"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHU0J_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1218269.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHU0J_003802"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHU0J_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHU0J_003803"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHU0J_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHU0J_003804"
     CDS             3787..>4168
                     /locus_tag="ACHU0J_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR1218270.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZPDE010000241         4155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPDE010000241 DAZPDE010000000
VERSION     DAZPDE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.247"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..753)
                     /locus_tag="ACHU0J_003805"
     CDS             complement(<1..753)
                     /locus_tag="ACHU0J_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFR1218271.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVH"
     gene            complement(811..1335)
                     /gene="sat2"
                     /locus_tag="ACHU0J_003806"
     CDS             complement(811..1335)
                     /gene="sat2"
                     /locus_tag="ACHU0J_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1218272.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1430..1903)
                     /gene="dfrA1"
                     /locus_tag="ACHU0J_003807"
     CDS             complement(1430..1903)
                     /gene="dfrA1"
                     /locus_tag="ACHU0J_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1218273.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2235..3212
                     /gene="intI2"
                     /locus_tag="ACHU0J_003808"
                     /pseudo
     CDS             2235..3212
                     /gene="intI2"
                     /locus_tag="ACHU0J_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(3400..3639)
                     /locus_tag="ACHU0J_003809"
     CDS             complement(3400..3639)
                     /locus_tag="ACHU0J_003809"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1218274.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1114 a          965 c          907 g         1169 t
ORIGIN      
        1 gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
       61 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
      121 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
      181 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
      241 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
      301 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
      361 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
      421 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
      481 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
      541 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
      601 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
      661 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
      721 agtcgatact tcggcgatca ccgcttccct catgatgttt aactcctgaa ttaagccgcg
      781 ccgcgaagcg gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca
      841 gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaacaggtc
      901 aatgccgccg agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt
      961 ttgtgtctct aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc
     1021 gatgagactg tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat
     1081 agaggctaga tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat
     1141 gaatgcgcca tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg
     1201 gtaggggctc acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa
     1261 cacttcacga acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat
     1321 caccgaaatc ttcatatgac gcctaacgcc tggcacagcg gatcgcaaac ctggcgcggc
     1381 ttttggtaca aaaggcgtga caggtttgcg aatccgttgc tgccacttgt taaccctttt
     1441 gccagatttg gtaactataa tttatgttag aggcgaagtc ttgggtaaaa actggcctaa
     1501 aattgctggg gatttcagga aagtaaacat caccttccgg ctcgatgtct attgtagata
     1561 tatgtagtgt atctacttga tcgatcaggc ttttgtatat ctccccacca cctgaaacaa
     1621 tgacatgatc cgttattttc tttaggttgg ttaaagcatc tttaattgat ggaaagatca
     1681 atacgttctc attgtcagat gtaaaacttg aacgtgttac gaccgcatac tttcggttgg
     1741 gtaatgctcc cattgattca aaagtcttgc gtccaaccaa cagccattgg ttataggtaa
     1801 tagctttaaa caggagctgt tcacctttgg cactccatgg aatatcaggg ccattcccga
     1861 taactccatt cttcgatata gctaccatta gtgatagttt cacaattctt cctcagaggt
     1921 taacatttta ttagtaagca tgctcgtttt gtcacccgct gatgcttacc gttaattaat
     1981 ataatcactt gtattaaata gactttcttt aaaaatacaa gacactctgt tattacaaat
     2041 cgtgcatgcc gtctatcctg cttgcacgat gcacattatc tcacccaaag aactcataac
     2101 attttaattt atttagcatt aaattaaaac tctaccggta aacaagcatc tctaggcgta
     2161 aaaaacaggc acttttttaa atcccagcta gaataggctg tataggcaga cagttgcaag
     2221 acaaggagcg ttttatgtct aacagtccat ttttaaattc tatacgcacg gatatgcgac
     2281 aaaaaggtta tgcgctgaaa actgaaaaaa cttacctgca ctggattaag cgttttattc
     2341 tgtttcacaa aaaacgtcat cctcagacca tgggcagtga agaggtcagg ctgtttttat
     2401 ccagcttagc aaacagcaga catgtagcca taaacacgca gaaaatcgct ttaaatgccc
     2461 tagctttttt gtacaacagg tttttacaac agccgttggg cgatattgat tatatccctg
     2521 caagcaagcc tagacggcta ccctctgtta tctctgcaaa tgaagtgcaa cgcattttgc
     2581 aggttatgga tactcgcaac caagttattt ttacgctgct gtatggtgca ggtttgcgca
     2641 ttaatgaatg cttgcgtttg cgggttaaag attttgattt tgataatggc tgcatcactg
     2701 tgcatgacgg taagggtggg aaaagcagaa acagcctact gcccacgcgc ctaatcccag
     2761 caataaaata actcattgag caagcgcggc ttattcagca agacgacaac ttacaaggcg
     2821 tagggccatc gctgcctttt gctttagatc acaaataccc ttctgcttat cgacaagcgg
     2881 cgtggatgtt tgtctttccc tccagcacgc tctgcaacca cccgtataac ggcaaattat
     2941 gccgccatca tctgcatgac tccgttgcgc gaaaggcatt gaaggcagcc gtacaaaaag
     3001 caggcatcgt tagcaagcgt gtcacttgtc atacatttcg tcactcgttt gctacgcatc
     3061 tattacaagc ggggcgtgat attcgcactg tgcaagaact cttagggcat aacgatgtta
     3121 agaccacgca aatctatacg catgtgttgg gtcagcattt tgccggcacc accagtcctg
     3181 cggatggact gatgctactt atcaatcagt aaaagtagat aaatatcttt atgtagccct
     3241 gagtagcccc tgatactttt tatggtgaaa aagataaaca atcatagagg ggataaatat
     3301 gagccagttc taccattgtt cagataagtt taagcaagat actgtcaatc acagtgacca
     3361 ttcatggtca ttacacttcc tccagttgcc agacaatgcc taacataaaa acgcaaaagc
     3421 ccaaaagtca gagcttaggt gttctaggcg ttttctaagc gctgaagaaa tacgtcttac
     3481 cgtagattgc gtgcgtttct gaagcacatt ttccttatac atggctttat cccaggcagc
     3541 ttcatcaagc tcttggagta acaagccagt aataatgcga cactctcggg tttgtagcga
     3601 gccgctggtg agatcggagt tatagcggaa aggggtcatt tgtaattatt tgtgggtgga
     3661 ggatagttaa cttattattg tagttggaag aggctgcttt ccaaacatta gcacctcgta
     3721 atgcttgtta ctgtatatta atttatgctc ttagcgcatg ttaatgggct attacaatgc
     3781 ataatgtcca catcaagata actgtggctt aacacctgta caagcgaaaa aaatcaatca
     3841 cctgtccgga aatacgggat cactgtaaag tgttggtgag gcagtctagg agggggattt
     3901 aatttgggat actgatgaaa ggattatgat cagttggata taggatcgta ctaagctgag
     3961 cggcaggtta gctagattag ttaaccagat aagtgaaatc tagttccaaa ctattttgtc
     4021 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     4081 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     4141 cgcccacagc tgccg
//
LOCUS       DAZPDE010000242         4095 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPDE010000242 DAZPDE010000000
VERSION     DAZPDE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.248"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            375..578
                     /locus_tag="ACHU0J_003810"
                     /pseudo
     CDS             375..578
                     /locus_tag="ACHU0J_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            597..3983
                     /locus_tag="ACHU0J_003811"
     CDS             597..3983
                     /locus_tag="ACHU0J_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1218275.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
BASE COUNT         1019 a          933 c         1276 g          867 t
ORIGIN      
        1 gttgttaata aaaagcttat agatttatag ttgataatta aaacgcatcc gatatttcag
       61 ctttttctat gcaagataca attttataag gtgtccattt ttatatggag gcggtagcgt
      121 gtctgttact agtggtttta tttgcatagt gaatatattg ggattgttaa atacataact
      181 atgtatccgc tctggtcgtt gtaaatattg ctaattgctt aatattatta gtgattatga
      241 ctggaaagat aaagtgtgct gtaaagcggt gatgggtatt acattcatga ttaaaaaaac
      301 agaacctgtc aataaataat tcctttatta aaacggacta tttacattat gacggaatat
      361 tatctcaacg aggcggtttg gaaatacaga gccagtttct ccggggtaac gagcaggtgt
      421 accagtgtca ggtggcattt gtattgccgg gtgagcgggt catgatggcc ttcacctacg
      481 ccagaaccac accgctgacc ccggcagata tgacgcgctg ggctgaaatt aaaaagaatc
      541 tgcgcttcag gatgcggcag gaagtcagga cgaattaatc atacagggaa cgggacatgg
      601 aagaggcatt ctgggcggca agacagggtg atgccctgct gcatacgtcg tttatggcag
      661 atgtgctggg ggcggtgctg gaggtggccg caaatgtcgt tattgatgcc ctcattgtcg
      721 gcacaacctc gttactggcg acgtcagtgg ggatcacgat ggggtgcacg gccgtcgtgc
      781 tgaccggttt tgtggcgggt gtggcgatgg tgtacaccgg cgtgacagaa aaggtcagcg
      841 aagcctgttc agccctcgca aatatgcttt tcccgcctca gattgaaggt tacatcctca
      901 ccggctctgg tgacacatgg atcaacagca aacctgccgc gcgggcagcg gcgactgcgg
      961 catcgcggca ggacatagag gcgcaggaag cgcaggcgaa ggcagaacag gaagaagcgc
     1021 agcgtcagga ggatgccaga acgttccggg atgtggccgg ggaatatctg gaaatggcgg
     1081 gggccatagc cctggcggtt aacccggtga cagggcctgt gctgatgtcc cgggctctgc
     1141 acgaccagct cagtacggaa gaagggcgtt ctgaaatggt ggatggcgtg acacattttg
     1201 tgtcggaact atggcagccc actgtggcca gtgcggcccc gggatccacc ccgacccctg
     1261 acgataaaat cgactgccat aaacatcctt cctcactgac gcagtttctc gcacaaaaaa
     1321 aagccgcctt tctggatgcc cccctgggaa cggcccttaa cctgctgaat ccgggcggta
     1381 tgctggaaac cgcctttcag ggagtgaatg cggttattgg cagcatcagt aatctgttca
     1441 aaggcgatga cgagccgccc gcagcggaat atatcgcgga aggcgcgcgg gatgtgcgta
     1501 tcaacagcca gccggcgaca cgcagcgggg cacgctgtac ctgcgaggcc agggtggtga
     1561 atgagccggg gaacggggcg tttgtgtcac cggatgtgcg cattggcggt ccgttactgg
     1621 tggtcaggga tatcagaagc ggcaggtcgc agataaccct ggtggcaacc gtggcgctga
     1681 tgtttcttcg cccgggcaaa atgctttcta aaatcgcctg ttttgctgcc agtgtcggga
     1741 tgagcatgct gacgcagaag gccaccagtg cccttccgca tccggtcaat gccgccaccg
     1801 gggccaaata cctggccgat gacgatgact tcgatttcag tctgccgggc cactttccgc
     1861 tggactggca gcgcgtgtac agcagtcgtg acgaacgtac ggaaggaatg tttggtcagg
     1921 gctggagtgt aatgtatgaa gtcagcctcg tgtgcacacc gggtagcgca gacgaaaact
     1981 gcatgacctt tgtgtcgggc atggggcgac gcttggatat ggaagccgta ctgccggggg
     2041 gcgggtttta cagccccggt gaagggctgg cggtaaggcg tggtgagcag gggcactggc
     2101 ttatcagcag cgatgacggg cagtgttttc tgtttgaggc tgacccgcac cacccgcagc
     2161 gccagcggct gaaaatgctg ggcgaccgta acagcaactg tctgaatctg tactacgacg
     2221 accggggccg cattgtggaa atcagcggcg aacagcagcg tccgtgcatc cgtctgtatt
     2281 acgaactggc ggcgcatccc cggcgggtga cacagattta tcagcatttc cctgagactg
     2341 cacctttact gctgcgtcgt tacagctatg acgaggccgg ccacctgaac ggcgtgtacg
     2401 acagcacggg gcacctgctg cgtgagtttg cttacgacga aaaccactgc atgacgctgc
     2461 accgccagcc gggcggcgaa gggtattatt accagtgggg ctggtatgaa ggtccggatg
     2521 acgcggcatg gcgggtgacg gggcaccaca cggacagcgg tgaacagtac cggctggcgt
     2581 gggacctggc gcagcgcagg ctgtgcgtga cggacggact ggggcgcaca cgttatcacc
     2641 agtgggatgc gcagaatcag gtgacggcgt accaggacga agccggacag gtgacgacat
     2701 tccgctggag cgacgaagaa cgactgttgc tggggatgac cgaccctcag ggcggcaaat
     2761 ggcgctatgt gtatgacctg caggggcata tcacggagac gcacgacccg ctggggcggg
     2821 tggagcaggc gcagtggcac ccggtgtggc accagccgga aacggaggtg gacgcggcgg
     2881 gaaacctgct ggggaggcgc gcgggggaaa gggccacggc agaaaacagc gtggtgccgt
     2941 tcaaccggct gatgtcttac cgtggtgtgc actaccgtta tgacgaattt ggccgtgcgg
     3001 tggaaaagga gggccggagc ggaacgcaga gttaccgtta tgatgcggaa caccggatgg
     3061 tggaagtgac gacagcgcgg gggacatacc ggtatgtgta cgatgcactg gggaggcgga
     3121 cggaaaaaca gcacatcagc cctgacggta agccgtacaa ccggacaaaa tttttatggg
     3181 acggaatgcg gctggcgcag gagagcaggc cggagggaac aggcagcctg tacatctaca
     3241 gggaccaggg gagttatgaa ccgctggcgc gggtggataa ggcgggcaaa gagggcccga
     3301 accggatact gtattttcac acggatgtga acggcgcgcc ggaagagatg acggacagcg
     3361 acgggaaaat cgtgtgggag acggggtatc aggtctgggg taacacgata caggagaagg
     3421 accacggcgg ggtggagcag aacctgcgct accagggaca gtacctcgac agggaaacgg
     3481 ggctgcacta caatctgcac aggtactatg atccggatgt cgggcggttc atggtaactg
     3541 acccgatagg gttgagaggc ggtttaaatc tctacagtta tgcacctaat cctctaaaat
     3601 atgctgatcc acttggatta actccatgtg ctgttagtaa ccaaaaagcg aatcgtttat
     3661 tagatagtag tgaaaccaaa gttactgtta ggtctagaag tgacgctgaa caattgttta
     3721 tggatagata tttaggtcat aattataaaa atatgacagg tgagtcaggc cccagcacta
     3781 agaacttaat ggaatatctt actgaaaata aaacaaaagc aggttcatat cattgggatg
     3841 atataaaaga tccttctgta acaaaacctt catatagagt gtcaggtcat gggccaggaa
     3901 atcctgatgg tgatttacca catttacaag ttcatcaaca tggtggaagt gttagacata
     3961 tatttttccc gtgggagaca taataatgaa caatctcaat acattattaa gtggaaaggt
     4021 tttgctcaaa gaaattagtc cattaatgcc tggatataga acatggattg aaatttcatt
     4081 gatcgatgaa ttaaa
//
LOCUS       DAZPDE010000243         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPDE010000243 DAZPDE010000000
VERSION     DAZPDE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.249"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHU0J_003812"
     CDS             complement(126..284)
                     /locus_tag="ACHU0J_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1218276.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHU0J_003813"
     CDS             complement(886..1077)
                     /locus_tag="ACHU0J_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1218277.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHU0J_003814"
     CDS             1142..1465
                     /locus_tag="ACHU0J_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR1218278.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHU0J_003815"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHU0J_003815"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHU0J_003816"
     CDS             2143..2673
                     /locus_tag="ACHU0J_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR1218279.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHU0J_003817"
     CDS             2680..2913
                     /locus_tag="ACHU0J_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR1218280.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHU0J_003818"
     CDS             complement(3459..3851)
                     /locus_tag="ACHU0J_003818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218281.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZPDE010000244         4023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPDE010000244 DAZPDE010000000
VERSION     DAZPDE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.250"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(28..285)
                     /locus_tag="ACHU0J_003819"
     CDS             complement(28..285)
                     /locus_tag="ACHU0J_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218282.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            741..1892
                     /locus_tag="ACHU0J_003820"
                     /pseudo
     CDS             741..1892
                     /locus_tag="ACHU0J_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1922..2350)
                     /locus_tag="ACHU0J_003821"
                     /pseudo
     CDS             complement(1922..2350)
                     /locus_tag="ACHU0J_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2865..3908)
                     /locus_tag="ACHU0J_003822"
     CDS             complement(2865..3908)
                     /locus_tag="ACHU0J_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1218283.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1119 a          887 c          940 g         1077 t
ORIGIN      
        1 gtagatacat acgcagtcag agtacactta ctttttatta tttcctgcaa tggcgattac
       61 ggctattgac agtaatgaag ttattagtga accaagacct aaagtagttc cgggaccaac
      121 accgttgtcg cttacggtta ctgacaggaa actgattgta ccagcgctta ttgtttgagt
      181 aaaacctgta atagatgcgg caaatcctgt ttttgaacca tttatttgta atgcgataaa
      241 attggctatt gggtaataaa aaccataggt tggataattt ttcaatcttt gataattgat
      301 ttccgattat gaaaaataaa actgagaagg agaaaataat tgagtaacta aaagtactca
      361 ggccataagt atgcatgaaa attgatggtg atattgatat gtagcaaaat agagaactga
      421 gtattaatga gacaagtata gtgccgcaga taaactcttt ggtcacaaag gatgcactgt
      481 tttgacatat tactttctca tttgcatgag ttatctttgg tgataataac attattataa
      541 tactgaacag catcataaca agaaatataa cattccagtt ataatattca ataatcaatg
      601 cgcgaggtgc tactaatggt attactgcgc ttacagatga tatatcagaa agcgcccgtt
      661 ttagttttaa tggagggtga tcttacccac caatagtgga cacaggacta agtgagtaaa
      721 ctctcaacca gaggtgactc atgacaaaac cagtatcaat cagcaagaag ccccgtaaac
      781 aacatacgcc tgaatttcgt aacgaagccc tgaaactcgc tgaacgcatc ggtgtggccg
      841 ccgcagcccg tgaactcagc ctgtatgaat ctcagcttta tgcctggcgc agtaaacagc
      901 agcaacaaat gagttcgtca gagcgcgaaa gcgaactggc cgctgaaaat gtccgcctta
      961 aacgacaact ggcggagcag gctgaggaac tgtccatcct ccaaaaggcc gcgacatact
     1021 tcgcgaagcg cctgaaatga agtatgtctt tatcgaaaat catcgggcag agttcagcat
     1081 caaagcgatg tgtcgtgtac ttcgggttgc ccgcagcggc tggtatgtct ggctcaggcg
     1141 tcgtcaccag atgagcctgc gccaacagtt tcggctcacc tgcgatgccg ctgtccataa
     1201 ggcattcttt gaggcaaagc agcgatacgg tgctccccgc cttgctgacg aactgccgga
     1261 gttcaatatt aaaaccattg ccgccagcct gcgtcgtcag gggctgcggg cgaaagccag
     1321 ccggaagttc agcccggtca gctaccgtgc acatggcctg cccgtattgg agaatctgct
     1381 ggagcaggac ttcagcgcca gtggcccgaa accagaagtg ggcgggtgac atcacgtact
     1441 tgcgtaccga tgagggctgg ttgtatctcg cagtagtcat cgacctgtgg tcacgcgccg
     1501 tcattggctg gtcgatgtca ccgcgaatga cagcacaact ggcctgtgat gcactgcaaa
     1561 tggcgttgtg gcggagaaga cgcccggaaa gcgtcattgt tcatacggac cgtggtggtc
     1621 aatactgttc aggggattat caggcaatgc tgaagcgaca caacctgcgt ggcagtatga
     1681 gtgcgaaagg ctactgttat gacaatgcct gtgtggaaag cttctttcat tcgctgaagg
     1741 tggaatgtat ccacggggaa cgctttagca gccgggaaat aatgcgggca acggtgttta
     1801 attatatcga gtgtgattac aatcgctggc gtcgtcacag tgcctgtggc ggtctcagcc
     1861 cggaacaatt tgaaaaccat aatctcgctt agggccgtgt ccacattacg tgggtaggat
     1921 cagtatgggt cgtcttgtat ttgtcatact gcccaatctc accaatcgaa aatgctcctt
     1981 ttggcaaagg ttcaagcagg tctctgggaa tggaggtcgc tggcgtcgga tataaaaaac
     2041 cctcacggct tagcaaggct ggcgtcacta tcggtgtaac aatatcaggc gtgcctgcgg
     2101 aacggtcctt accatgctgc agtaaccgga aataaacttt tgggcgtaag atgacattga
     2161 cagtttcgac atcgtctttt tctcccttaa aaaatttatt gacaatcgct tcgtcaagac
     2221 gtcccgccgc tatcccatgc cagtgagtga aattttgggc gtctttccgt tcaaaacagc
     2281 cctttcctga ctcagcatct gcaagcgagt tgcgccagta tgaggcaaac cttaaagcat
     2341 tttcatccat taccagtcct gtgactcaat gaaatccatt gatttatcga atataaaacc
     2401 accgtatgga caactattgt ccatattaaa aaagttggat gcaatgtttt gtttataatt
     2461 cgatatcaaa tgtactggtt gtacgaggtt cagaacacaa gcacattcaa cagtacgtca
     2521 ggactattga tttcataaca gcgaatacaa ttttttactg tttataatgg ctgcctgtgt
     2581 tggacgggga taccagttca accgaaaacg ccagaggagg ggattacccg ctggcagggt
     2641 aaatctgtgg tttttgatgc cagctataac gtgtttgacg ataaagtatt acgaaagaac
     2701 tttaatgagt tcatggagta attcgttaaa gtaatttaca acgctatcct tttgcaaagg
     2761 aaactttcag aaaataaccc cagggtaatc acctggggaa ggaacacagt gttattattg
     2821 gataataaat tattacttgt tttaaagtta cactaatgca atatctactg atactggatg
     2881 cttgaatgtt tcatccctgc tgcggaaaag ggcaatatcc agggccagat atattcaccg
     2941 ttcatatttc tgcatttctg tacaatttcc tcagaaagtg tcttgtcaat tcttttccac
     3001 aggtgctcct gctgtcttaa aatactttcg catatttcga aatcatcttc ctgaatcagc
     3061 atttcggcgc atttaatgat ttctgatcgt tcatgcagca cggaggaatg gttatagagc
     3121 cagtttacaa acagcaatgc ttccggatat tgtccccata atggtgagta tgttgcatag
     3181 taatcttctg ccgtatccag agagccggag aagtcatgcc acacagcaag tggaaatgga
     3241 tgcagatttc cgcacagcat ttctgcgatg taagtgtttg tggcaaagcc atggcgaaaa
     3301 gctgtggcgg ccttaacatg ttccccgttc agaaaatgca ggagaccgag ttcataccag
     3361 taaggtgaaa attcatcagc atgctctttc agtacactga aggctcgctc atgatcgccg
     3421 gtgcgtaaca gctctgaacc caacaaccag cgagccccct gattatcttc aggattgtat
     3481 gccagtattt tatcagtgag cataaccgca tcctgatggc gctgtaagtg cacatttgcg
     3541 agaattgctg cataaagcgc gcgcagataa ggccggtttt cgggatgcat ccagattatt
     3601 tcaccacaga aactttccgg gatgatacga ttacctgccg ccagtccttt aagagcggcg
     3661 ttcagtgcct tacgcggggc attctgttcc agaaatgcat atgccagatg agcgtgtata
     3721 tcaataaagt ccggctcccg ttgagcaagg cgtcggagtt tgttgatgta actcgtctcg
     3781 ctgatttcat cagcgtcgta tgcatcaagc acttcttcaa actcccccag caatgggcca
     3841 aaccaggtat ccggaaatac gaaataaccc ttgttatcgt gcacttcaaa gcataatcta
     3901 tcgttcattt tctgagcctg taaaacatgt ggtgatatag tctgagagca taaaagttga
     3961 gattctgtgt atcaagtcct gtcttggcac cgaatgaata ttgcttacga agccccttca
     4021 tgc
//
LOCUS       DAZPDE010000245         3976 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPDE010000245 DAZPDE010000000
VERSION     DAZPDE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.251"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHU0J_003823"
     CDS             <1..224
                     /locus_tag="ACHU0J_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1218284.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHU0J_003824"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHU0J_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1218285.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHU0J_003825"
     CDS             518..1108
                     /locus_tag="ACHU0J_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1218286.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHU0J_003826"
     CDS             1348..1608
                     /locus_tag="ACHU0J_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1218287.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHU0J_003827"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHU0J_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1218288.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHU0J_003828"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHU0J_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1218289.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACHU0J_003829"
     CDS             3457..3648
                     /locus_tag="ACHU0J_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218290.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3878..>3976)
                     /locus_tag="ACHU0J_003830"
     CDS             complement(3878..>3976)
                     /locus_tag="ACHU0J_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218291.1"
                     /translation="GSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1074 a          988 c          988 g          926 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacatc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt
     3841 ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg
     3901 ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa
     3961 aacgcatctg actccc
//
LOCUS       DAZPDE010000246         3840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPDE010000246 DAZPDE010000000
VERSION     DAZPDE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.252"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            248..694
                     /locus_tag="ACHU0J_003831"
     CDS             248..694
                     /locus_tag="ACHU0J_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1218292.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            705..2156
                     /locus_tag="ACHU0J_003832"
     CDS             705..2156
                     /locus_tag="ACHU0J_003832"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1218293.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2146..3216
                     /locus_tag="ACHU0J_003833"
     CDS             2146..3216
                     /locus_tag="ACHU0J_003833"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1218294.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3216..>3840
                     /locus_tag="ACHU0J_003834"
     CDS             3216..>3840
                     /locus_tag="ACHU0J_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR1218295.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          879 a          991 c         1022 g          948 t
ORIGIN      
        1 aaaattaaac atattacatc tcccggtaag ttatatttcc ctgatacatt gtgagtaaat
       61 cacaaaaata atgaataacc cattaatgat tcatgtggtt tatttaaata accattatgt
      121 gcattactcc gcaaatctga cctttcactc tgtctcaaat tgtcattgtt taccgacata
      181 tcgcctctca tcacttaatc gctccaccat tacaattgat taaaatatat tttatggagt
      241 ccgattaatg gcagctctta ctgcaagctg tattgacctg aatattcagg gcaatggcgc
      301 ttattccgtt ctgaagcagt tggcgacaat agcgttacaa aacggtttta tcaccgactc
      361 acaccagttc ctgcaaaccc tgctgctgcg cgaaaaaatg cactctactg gatttggttc
      421 cggtgtcgca gtgccgcacg gtaaaagcgc ctgcgttaaa caaccgttcg tattattcgc
      481 ccgcaaagcg caggctattg actggaaagc cagcgatggc gaagacgtca attgctggat
      541 ctgcctcggc gtgccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg
      601 tcgcaaaatt attcaccagg aatttattca tcaactgcaa cagggcgata ccgaccaggt
      661 gctggccttg ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag tcatccttac
      721 gtattgtcgc gatcaccaac tgccccgccg ggatcgctca cacctacatg gtggcggaag
      781 ccctggaaca gaaagcccgt tctctcggcc ataccataaa agtggaaact caagggtcca
      841 gtggcgttga aaaccgctta tccagcgaag agattgccgc tgccgattac gtcattcttg
      901 ctaccgggcg tggcctgagc ggtgatgatc gcgcgcggtt tgccgggaag aaagtttatg
      961 agattgccat ctccctggcg ttgaaaaata tcgaccagat tttcagcgaa ttaccgacaa
     1021 actcgcagct ttttgccgca gatagcggcg tgaagcttgg caaacaggaa gtgcaaagcg
     1081 gcagcgtgat gagccacctg atggcgggcg tctctgcggc gctgccgttt gtcatcggcg
     1141 gtggtatcct ggtggcgctt gccaacatgc tggtgcagtt cggtttgccg tataccgata
     1201 tgtcgaaagg cgcgccgtcg tttacctggg tagttgaatc catcggttac cttggcttca
     1261 cctttatgat ccccatcatg ggcgcttata ttgcctcgtc gattgccgat aaaccggctt
     1321 tcgctccggc gtttctggtt tgctatctgg cgaacgacaa agcgctgctc ggcacccagt
     1381 cgggtgcggg attcctcggc gcggtggtgc tggggctggc gattggctat ttcgtcttct
     1441 ggttccgcaa agtgcgcctt ggcaaagcat tacaaccgct gctcggttca atgctgatcc
     1501 cgttcgttac tctgctggtt ttcggcgtgc tcacttacta cgttatcggg ccggtgatgt
     1561 ccgacctcat gggcgggctg ctccacttcc tgaataccat tcctccatca atgaagtttg
     1621 cggcagcgtt tctggtcggt gcgatgctgg cgttcgatat gggcggcccc atcaataaaa
     1681 ccgcctggtt cttctgcttc tcactgctgg aaaaacacat ttatgactgg tacgccatcg
     1741 tcggcgttgt cgcgttgatg ccgcccgttg cggcgggtct tgcgacgttc attgcgccca
     1801 aactgtttac ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc ggtgccacag
     1861 tcgcaaccga accggctata ccttacgctc tggcagctcc gttgccgatg attaccgcca
     1921 atacgctggc gggcggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg
     1981 cacctggttt gggtatcttt gacccgctga tcggcctgat gtcgccggta ggttcgtttt
     2041 atctggtgct ggcgattggc ctcgcgctga acatttcatt cattatcgtc ctgaaaggat
     2101 tgtggttacg ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa cattgagtta
     2161 ctgcaacagt tgtgcgaagc cagcgccgtt agcggcgatg aacaggaagt tcgcgacatc
     2221 ctgataaaca cgctggaacc ttgcgttaat gaaatcacct ttgatggtct gggcagcttt
     2281 gttgcccgta agggaaataa aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc
     2341 ggctttatgg tcacccacat cgacgagagc ggttttctgc gtttcaccac cattggtggc
     2401 tggtggaatc agtcgatgct caaccatcgg gtaaccatac gcacacacaa gggagtgaaa
     2461 atccctggtg tgattggttt cgtcgcgcct catgcgttaa cggaaaagca aaggcaacag
     2521 ccgctgtcat ttgatgagat gttcattgat attggcgcaa acagtcgcga tgaggtggaa
     2581 aaacgcggtg tggcgatggg cgattttatt agcccggaag ccaattttgc ctgctggggc
     2641 gaagataaag tggtcggcaa ggcgctggat aaccgcatcg gctgcgcaat gatggctgaa
     2701 ctattgcaga cggtgaataa tcccgaaatt acgctgtatg gcgtcggcag cgtggaagaa
     2761 gaagttgggc tacgcggggc acaaacctcg gcggaacaca ttaaaccgga tgtagtgatc
     2821 gtgctggata ccgctgtcgc aggcgatgtt ccgggcattg ataacattaa atacccgctg
     2881 aaactgggcc aggggccggg gctgatgctg tttgacaagc gctacttccc caaccagaaa
     2941 ctggtagcag cgttaaaaaa ctgtgccgca cataacgatt taccgctgca attttccacc
     3001 atgaaaaccg gagcgacgga tggcgggcgc tacaacgtga tgggcggcgg gcgtccggtt
     3061 gttgcgctgt gcctgccaac ccgctatctg cacgctaaca gcggcatgat ttcaaaagcc
     3121 gattacgatg ctctgctcac gttgatacgg ggttttctga cgaccttaac cgcggagaaa
     3181 gtcaacgcgt ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga
     3241 ttgtcgatct gctggagcaa cagccgcgca cgcctggtga gctggcgcaa cagactggcg
     3301 tttcaggcag gaccatcctg cgtgatattg actatctcaa ctttaccctt aacggcaaag
     3361 cccgcatttt cgccagtggc agtgcgggct atcagctgga aatcttcgag cgccgcagct
     3421 tttttcagtt gctgcaaaag cacgataacg acgatcggct gctggcgctg ttattactga
     3481 atactttcac tccccgtgcg caactcgcct cggcgcttaa tttgccagaa acgtgggtag
     3541 cagagcgtct gccccagtta aaacagcgtt atgaacgcac ttgttgcctg gccagccgcc
     3601 ctggtttggg ccatttcatt gatgagacag aagagaaact cgttatcttg ctggcgaact
     3661 tgctgcgcaa agatccgttt ttaattccgc tggcgggcat aacacgagac aaccttcagc
     3721 atttatccac ggcctgcgac aaccaacacc gctggccgct catacagggt gattatctct
     3781 ccagcctgat tctggcgatt tacgccctgc gtaatcaact gaccgatgag tggccgcaat
//
LOCUS       DAZPDE010000247         3808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPDE010000247 DAZPDE010000000
VERSION     DAZPDE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.253"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHU0J_003835"
     CDS             complement(24..278)
                     /locus_tag="ACHU0J_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1218296.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHU0J_003836"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHU0J_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1218297.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHU0J_003837"
     CDS             1228..2694
                     /locus_tag="ACHU0J_003837"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1218298.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHU0J_003838"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHU0J_003838"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1218299.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
BASE COUNT          936 a          969 c          983 g          920 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgc
//
LOCUS       DAZPDE010000248         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPDE010000248 DAZPDE010000000
VERSION     DAZPDE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.254"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHU0J_003839"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHU0J_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHU0J_003840"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHU0J_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHU0J_003841"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHU0J_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHU0J_003842"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHU0J_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZPDE010000249         3800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPDE010000249 DAZPDE010000000
VERSION     DAZPDE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.255"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..247
                     /gene="ypdI"
                     /locus_tag="ACHU0J_003843"
     CDS             <1..247
                     /gene="ypdI"
                     /locus_tag="ACHU0J_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1218300.1"
                     /translation="FLLVSIMACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQP
                     SQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(324..566)
                     /locus_tag="ACHU0J_003844"
     CDS             complement(324..566)
                     /locus_tag="ACHU0J_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1218301.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            919..1839
                     /gene="lpxP"
                     /locus_tag="ACHU0J_003845"
     CDS             919..1839
                     /gene="lpxP"
                     /locus_tag="ACHU0J_003845"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1218302.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2194..2265
                     /gene="ypdK"
                     /locus_tag="ACHU0J_003846"
     CDS             2194..2265
                     /gene="ypdK"
                     /locus_tag="ACHU0J_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1218303.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2330..3568)
                     /gene="alaC"
                     /locus_tag="ACHU0J_003847"
     CDS             complement(2330..3568)
                     /gene="alaC"
                     /locus_tag="ACHU0J_003847"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1218304.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT          994 a          965 c          879 g          962 t
ORIGIN      
        1 atttttattg gtctctatta tggcatgcaa tgtttttgca ttttccattt ctggtggtgt
       61 aagtgagagg agctataaag agactgaaaa aacatcagcg atgacgacca cacactctac
      121 aaaacttcag ccatcacagg cgattttgtt taagatgaga gaagatgtgc caccattaaa
      181 cctcacagaa gaaatcacgc ctacttatcc gacaaaggcg aattatctta ttcatcctgt
      241 gcgatagcgt ctgtcacatg gcaggtgaac aatcatgtct gatgctgatg tgaacacctg
      301 cccaaatgag ccatcctgac ccactatcgc ctgccatacc ccaccataaa gcgaatcatc
      361 aagccacaag ccggggcaag aaacataaat atccccgtca ccagttcatt accgccagtt
      421 aaccacatgg tgaagtaata aatgagtgca gcgaggatca ccatgccgaa gaaagaagag
      481 accgccgata ccacgttcag tacctgaccg atgtagccgc ttacgcagac aatacacagg
      541 gcgagaaaca tccataaata aatcatagct caacctcctg ccgcgctaaa aaacctgtaa
      601 cttcagtcac tttaatagta agacaatcct taacctctgg caatttttgc taagcatcaa
      661 tgaaaacagc acgttaaatg caacgctatt gcgtgcggaa aattttttac tttgcacgat
      721 taataatcag tagctgaaag cagtcagcca gtgataaagt agagccaccg cattcatgct
      781 cctcgtggtt atgtcctgac tagtctttac actctttaca ggaaccattg tcgtacatga
      841 tggcccaacc aattgagtgt accgctgtcg acatggaatc ttctgcgata caacaattcg
      901 tatctacaga aggtaactat gtttccacaa tgcaaatttt cccgcgagtt tctacatcct
      961 cgctactggc tcacatggtt tgggcttggt gtactctggc tttgggtaca gcttccttat
     1021 cctgttctct gctttctcgg cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt
     1081 cgtgaatcta tcgcccgtaa aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa
     1141 cgcgagaaga tgattgccga aaactttcgt tcactcggca tggcgctggt agaaaccggc
     1201 atggcatggt tctggcccga cagtcgcgta cgtaaatggt ttgatgttga agggttggat
     1261 aaccttaaac gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg
     1321 tcgctggaac tgggcggccg cgtgatggga ctgtgccaac caatgatggc tacctatcgt
     1381 ccacataata atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa
     1441 gcgatgatcg gcagaaataa tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg
     1501 gtatggtttg ctcccgatca ggattatggt cgtaaaggca gctccttcgc gccgttcttt
     1561 gcggtggaaa atgtcgccac aaccaatggc acctatgttc tctcccgtct ctctggcgca
     1621 gccatgttga ccgtaacgat ggtaagaaaa gcggattaca gcggatatcg tttgttcatc
     1681 accccagaga tggaaggcta cccgacagat gaaaatcaag ccgctgctta tatgaacaag
     1741 attatcgaga aagagatcat gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt
     1801 aaaacgcgcc cggtgggaga atcgtcgttg tacatttaac aaactgctct tactctttcc
     1861 ctcctcaaat cggccataat aacctcatga tttttatagc ttttttctta agttttttcc
     1921 ttcgctgaat atctaattat taccgtcaga aattaaactg tcgccgatat gagacacttg
     1981 taagttacta aaagtaaata agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat
     2041 tagcgccgtc tcgagcagtt tagctcagaa ttctggggtg atgcccacgg taaaatacag
     2101 ggcttttaag gtcgctttgc ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc
     2161 aggcgtaaca gtaattacgc ggagagatgt aaagtgaaat atttctttat gggcatttct
     2221 tttatggtca tcgtttgggc cggtactttt gccctgatga tctaaaagca aactgtgcaa
     2281 tgaaaaacag gagccatcag gctcctgttt gcatttctga cgacggtgtt tattccgcgt
     2341 tttcgtgaat atgtttgctg ctgacgggta gtaaaccgtc ggcacggaac atcgctttaa
     2401 tccctctgat ggcctgacga atacggtcgt ggttttcaat cagtgcaaag cgaacatgcg
     2461 tatcaccata gtcgccaaag ccaatccccg gcgagacaca aaccttcgct tcgttaagga
     2521 gcttcttggc aaattccagc gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga
     2581 catacatcga agccttcggc atttcgacca tccagcccgc ttcatgcagc cctttaacca
     2641 atacatcacg acggcgtttg tactgttcgg caatgtcgcg cacgcactgt tgatcgccct
     2701 cgagtgccgc aatcgccgcc acctgcaacg gcgtaaaagt gccgtaatcg tgatagcttt
     2761 tgattcgcgc cagggcactg accagcgttt tgttgccgac cataaagccg atacgccagc
     2821 ccgccatgtt gtagcttttc gacagcgtaa agaactcgac cgccacatcg cgtgcacccg
     2881 gcacctgcat aattgacggc gctttccagc catcgtagac gatatcggca taggccaggt
     2941 catggaccac cagcacatcg tagcatttcg ccagcgccac aaccttttcg aagaactcca
     3001 gttccacgca ttgcgcggtt gggttcgacg ggaagccgag gatcatcatc ttcggtttcg
     3061 gataactttc acgaatggcg cgctccagtt cgttgaagaa atcgacacct tccaccagcg
     3121 gcactgagcg tacctgcgcc ccggcaatca ccgcgccata aatatggatc gggtaacttg
     3181 gattcggcac cagcaccgtg tcaccatgat ccagcgtcgc cagcatcaga tgcgccaggc
     3241 cctctttcga accaatagtg acgatggctt ctgattccgg gtcgatttca acgtcgtagc
     3301 gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt
     3361 aaccatgcgt gtccgggcgc tgggccacag tacataattt ttcgacgata tgcggcggag
     3421 tcgcaccgtc cgggttaccc atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag
     3481 ccattttcag ttcagcggtg atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa
     3541 agcgacgttc agggcgagtg tcagccataa tttcctcaga ttaacgttag cgcccggacc
     3601 gtccgagcga cgctgccaca atggtggctg ttttgaaaat agcctgatta atttctgtct
     3661 gtccagcgtt ttagaacatt tttatcaatg gatttaaatg aggaactcca cttcaaaaac
     3721 aggaaatttt tctacgctaa caagaaaatc accaccaatt ttattaacat tataatttca
     3781 ataaatttac cagccagaag
//
LOCUS       DAZPDE010000250         3770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPDE010000250 DAZPDE010000000
VERSION     DAZPDE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.256"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            2..256
                     /gene="yoeB"
                     /locus_tag="ACHU0J_003848"
     CDS             2..256
                     /gene="yoeB"
                     /locus_tag="ACHU0J_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1218305.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            339..1163
                     /locus_tag="ACHU0J_003849"
     CDS             339..1163
                     /locus_tag="ACHU0J_003849"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1218306.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            1209..2192
                     /locus_tag="ACHU0J_003850"
     CDS             1209..2192
                     /locus_tag="ACHU0J_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1218307.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHU0J_003851"
     CDS             2353..2415
                     /gene="yoeI"
                     /locus_tag="ACHU0J_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1218308.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            2405..3763
                     /gene="plaP"
                     /locus_tag="ACHU0J_003852"
     CDS             2405..3763
                     /gene="plaP"
                     /locus_tag="ACHU0J_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1218309.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          883 a          893 c          997 g          997 t
ORIGIN      
        1 agtgaaacta atctggtctg aggaatcatg ggatgattat ctgtactggc aggaaacaga
       61 taagcgaatt gttaaaaaga tcaatgaact tatcaaagat acccgcagaa caccatttga
      121 aggtaagggg aaaccagaac ccctgaaaca taatttgtca ggtttctggt cccgacgcat
      181 tacagaggag caccgtctgg tatacgcggt tactgatgat tcactgctca ttgcagcatg
      241 tcgttatcat tattgaacag attcattcat agaatgaatt gtcagttttg atacgctagc
      301 gtagccttat agatctaagg tgtatcagga gataacggat gaaaaaggtc gcaattgtcg
      361 ggttagggtg gttaggtatg ccgctggcga tgtcactttc agcgcgaggc tggcaagtca
      421 ccgggagtaa aaccacacaa gatggtgtcg aagcggcccg aatgagtggc attgatagct
      481 atctgcttcg catggagcct gagttagttt gcgattctga cgatctggat gcccttatgg
      541 atgccgatgc gctggtcatt acgcttccgg cacgtcgtag cggccccggc gatgagttct
      601 atttacaagc ggtacaagag ttagtggata gcgcgctggc ccatcgtatt ccccgaatta
      661 tttttaccag ctcaacatct gtctatggcg acgcgcaagg cacggttaaa gaaactaccc
      721 cgcgtaatcc ggtaaccaac agtggacgag tattagaaga actcgaagac tggctgcaca
      781 atttacccgg tacttcggtc gatattctgc gtcttgcggg cctggtcgga ccgggacgtc
      841 atccgggacg cttctttgcc ggaaaaaccg cccctgatgg tgaacatggt gttaatttag
      901 tccatttaga agatgttatt ggcgctatca ctctgttgtt acaggcacct aaaggcggac
      961 acatctataa tatatgtgcg ccagctcacc ctgcgcgtaa tgttttctat ccgcagatgg
     1021 cccgtttact ggggctgcaa cctccgcagt tcagaaatag tctggacagc ggcaaaggta
     1081 agattattga tggcagtcgg atttgtaatg aactgggatt tgaataccag tatcccgatc
     1141 cgctggtaat gccgttggag taaacgatca cgccagcagg acgccgcatg cactgcaagg
     1201 gggcgtgtat gaaaccactg ctggatgtgt tgatgatcct cgatgccata gaaaaagagg
     1261 gcagttttgc ggcggcgtca gccaaactct acaagacgcc gtccgctctg agttacaccg
     1321 ttcacaagct ggaaagcgac ctaaatattc aattgcttga tcgtagcgga caccgcgcta
     1381 aattcacccg caccggaaaa atgttattag agaaagggcg ggaagttctg cataccgtgc
     1441 gagaactgga aaagcaggcg atcaaactgc atgaaggctg ggaaaacgag ctggtgattg
     1501 gcgtggacga cacgtttcct ttttctctgc ttgcgcctct cattgaagct ttttatcaac
     1561 atcatagcgt gacgcgcctg aagtttatca atggcgtact cggtggttcc tgggatgccc
     1621 tgactcaggg gcgagcggat attatcgttg gcgcgatgca tgagccacca tcttccagtg
     1681 agtttggttt ctcgcggttg ggcgatcttg aacaagtctt tgcggtcgcg cctcatcatc
     1741 cgttagccca ggaagaagaa ccgttaaacc gtcgtatcat taaacgatat cgggcgattg
     1801 tggtggggga tactgcgcag gcaggcgctt ctacggcctc acaacttctc gacgaacaag
     1861 aagccattac cgttttcgat tttaaaacca aactggagct gcaaattagc ggccttggct
     1921 gcggctattt accacggtat ctggcgcaac gttttctcga tagtggcgcg ttaatcgaga
     1981 agaaagtggt cgcccaaact ctctttgaac ctgtctggat tggctggaat gaacagaccg
     2041 cagggcttgc cagcggctgg tggcgagatg aaattttagc aaatagtgcg atcgccggtg
     2101 tttatgcaaa atctgatgac ggaaaatcag ccattcaaag aaaaattatt atgacaagcc
     2161 tctcattctc ttgtcatttc ccccccattt aggcacaatg cgccgctgtc aaaaaatgat
     2221 taaaaaccga cgtttaatta gcgtcggtta ttttttgctt caaaccaatc attcatacca
     2281 agaggccggg cttcgtaccg gatagatatt tactaaaaat cgacagttgt tgtcgctgag
     2341 gaatccaaaa aaatggggca attttttgct tacgcgacgg ttatcaccgt aaaggagaat
     2401 aaccatgtcg cataacgtta ctccaaacac ctctcgcgtg gaattgcgta aaacgcttac
     2461 gttagttccg gttgtaatga tgggtctagc ctatatgcag ccgatgacgc tgtttgatac
     2521 atttggtatc gtttcaggcc tcacggatgg tcatgtgccg acagcctatg cgttcgcatt
     2581 gattgcgatc ctgtttacgg ctctgagcta cgggaagctg gttcgccgct atccttctgc
     2641 tggctctgca tacacttacg cccagaaatc cattagcccg actgtcggct ttatggtggg
     2701 ttggtcttct ctgctcgact atctgttcgc gccgatgatc aacattctgc tggcgaaaat
     2761 ctattttgaa gctctggtgc cttccattcc atcgtggatg tttgtggtgg cgctggtggc
     2821 ctttatgacc gcctttaacc tgcgtagtct gaaatccgta gcgaacgtca acactgtaat
     2881 cgtcgtgttg caggtagtgc tgatcgcggt gattctgggc atggttgttt atggcgtatt
     2941 tgaaggtgaa ggcgctggta cgctggcgag cacgcgtccg ttctggtctg gcgatgcgca
     3001 tgttatcccg atgattaccg gggcgacaat cctgtgcttc tcctttaccg gctttgacgg
     3061 catcagcaat ctgtcggaag aaaccaaaga tgcagagcgc gtgatcccgc gtgcgatttt
     3121 cctgaccgcg ctgattggcg gcatgatctt catctttgca acttacttcc tgcagctgta
     3181 cttcccggat atctctcgct ttaaagatcc ggatgcgtca cagcctgaaa tcatgctgta
     3241 cgttgcgggt aaagcgttcc aggttggcgc gctgatcttc tccaccatta ccgtactggc
     3301 gtccggtatg gcggcgcacg caggcgtagc gcgtctgatg tacgtaatgg gtcgtgacgg
     3361 cgtgttcccg aaaagcttct tcggttatgt acacccgaaa tggcgtactc cggcgatgaa
     3421 catcatcctg gttggcgcga ttgccctgct ggcaatcaac ttcgacctgg taatggcaac
     3481 ggcgctgatt aactttggtg cgctggtggc gttcaccttt gttaacctgt cggtcatctc
     3541 gcagttctgg atccgtgaga agcgtaacaa gacgctgaaa gatcacttcc agtatctgtt
     3601 cctgccgatg tgtggtgcgc tgaccgttgg tgcgctgtgg gttaacctgg aagaaagctc
     3661 aatggttctg ggtctgatct gggctgctat cggtctgatt tacctggctt gcgtcaccaa
     3721 gagcttccgt aatccggttc cgcagtacga agacgtagca taatcatctt
//
LOCUS       DAZPDE010000251         3765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPDE010000251 DAZPDE010000000
VERSION     DAZPDE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.257"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1041
                     /gene="tsuA"
                     /locus_tag="ACHU0J_003853"
     CDS             <1..1041
                     /gene="tsuA"
                     /locus_tag="ACHU0J_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1218310.1"
                     /translation="SGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAIS
                     AQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIG
                     SWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLW
                     VVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRM
                     FGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRAADAQTT
                     LRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRP
                     QRKARLATAAAN"
     gene            1055..1282
                     /gene="tsuB"
                     /locus_tag="ACHU0J_003854"
     CDS             1055..1282
                     /gene="tsuB"
                     /locus_tag="ACHU0J_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1218311.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1325..2749)
                     /gene="sbcB"
                     /locus_tag="ACHU0J_003855"
     CDS             complement(1325..2749)
                     /gene="sbcB"
                     /locus_tag="ACHU0J_003855"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1218312.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2931..>3765
                     /locus_tag="ACHU0J_003856"
     CDS             2931..>3765
                     /locus_tag="ACHU0J_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1218313.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
BASE COUNT          895 a          888 c          991 g          991 t
ORIGIN      
        1 agcgggctaa tttgtggtgc tctgctggga tttgtcatgc agcgtgggcg tttttgcctg
       61 actggtggtt ttcgcgatat gtatatcgcg aaaaataatc gcatgtttta cgccttgctg
      121 attgcaattt cggcacaaag cgtgggggtt tttgcgttaa ttcaggcggg cctactgact
      181 tacgaagccg gggcgttccc gtggcttggt actgttatag gtgggtatct tttcgggctg
      241 ggaattgttc tggcgggtgg atgtgccacc gggacctggt atcgcgcggg tgagggattg
      301 atcggcagtt ggatcgcgct tttcacttat atggtgatga gtgcggtgat gcgttctcca
      361 cacgccagtg gtttaaatca aaccttgcag cactacacta ctgaacataa ctctattgct
      421 gagactttta atttgtctgt gtggccgttg gttaccgttt tgctggtgat aacgctctgg
      481 gtggtgatga aagagttgaa gaagccaaaa ctcaaagtcg cgaccttacc gccgcgccga
      541 accgggatcg ctcatattct gtttgaaaaa cgctggcatc cctttgtcac tgctgtactc
      601 atcggtttga ttgcgctttt agcctggccc ctgagtgaag caaccggacg catgtttggg
      661 ttaggagtca cttccccaac ggccaatatt cttcaatttc tggtcgcggg tgacgtgaaa
      721 tacattaact ggggcgtttt cctggtgtta gggatcttcg tggaggcatt tattgcagcc
      781 aaagcgagcc gtgagttccg cgttcgcgca gctgatgcac aaacaacatt acgtagcggg
      841 ttaggtggtg tactgatggg cttcggtgcc agtattgcag gtggttgctc tatcggtaat
      901 ggactggtta tgactgcaat gatgacctgg cagggctgga ttggccttgt atttatgatt
      961 ctcggagtct ggactgcgtc ctggcttgtg tatgttcgac cgcagcgtaa ggcgcgactg
     1021 gcaactgctg cggcaaatta agtaaggtgt aaagatggcg attaaaaagc tggatgtggt
     1081 gacgcaggtc tgtccgtttc cactcataga agcaaaagca gcactggcag aaatggccag
     1141 cggcgatgag ttggtaattg agtttgattg cacccaggca actgaagcaa tcccacagtg
     1201 ggcggcagaa gaaggacatg ccatcaccga ttatcagcag attggcgatg cagcgtggag
     1261 catcaccgtt caaaaagcct gactcaacat tgtcctccgc cgtaccagcg gcggcggctt
     1321 caaattagac gatctcttcc gcgtactgcc aaagtgcttt taacagcgcc actttctctt
     1381 tgtcatcggc atattgttgt accagcatct gcaattcatc agcataaccc tgcaaaaact
     1441 ctggcgtgaa gacctggtgg cggtgctcca gccagcgttg ctgctcggca taatccagcg
     1501 tccccgggaa gttgcgtgcc cgataattga acaacaactt ttcaattcgt ttatcaacaa
     1561 aagtgatatc cagtgccggt aaattacgcg gctcggtttc cagcacaatt ttcattgctg
     1621 cacgatcggc gtcactgaaa aagccattat aaagctgtgc atccacatta tctgaaggtg
     1681 taaacggttc ggcttccgcg aatatcgcta ccactttttc acgcacttgc ggattttcac
     1741 gcagaatttt taggttatcg aggcaatgct gacgattaat tcccagtcgg tcggcatctt
     1801 ccgggcgtag cgtattcgcc tgggccagta ccggacattt attgatatgc accagcttaa
     1861 ccggaacggc ggcgttatcg ccaagatcgg cttttgcggt atataaacgc tcgcgcaatg
     1921 tgtcgatatc cagctccagt aatggcgaaa tgtctcctgc caaatccgcc ataattacgg
     1981 cattgcgatt ttcaggatgc cacgccagcg gtgccaccca gctggtattg ccacgccatg
     2041 ccccaaacat tccggaaacg tgagccaggg gtttcatctg cggaacatca atcaacgcca
     2101 tcagtttgtg tttattacga tgggtaaaga gataatcaaa caggcgtggc tgacgcgttt
     2161 ttaccagttt cgccatcgca atagtggcgt acacatcagc catcgcatcg tgggcgttgc
     2221 tatgttcaat accattcgct ttggttaaat gctcaaggcg aaagctcggt agaccgtcat
     2281 cattttcagg ccagtttatt ccttccgggc gcagggcata acaggcacgc ataacatcca
     2341 gtaaatccca gcgcgagtta tcatgctgcc agctccaggc gtaaggatcg tagaaattac
     2401 gataaaaaac gttgcgtgtg acttcgtcgt cgaaacgcac attgttgtag cccagaatac
     2461 aggtcttcgg tacggtaaaa agcgagtgaa tacgggcggc aaacgcggct tcgttttctc
     2521 ctttcgcccg tgcctcctgc ggggtaatac cggtaattaa tactgctcca ggccggggta
     2581 aatagtcatc cgcgggcttg cagtaaaaga cttcgggttc gccgatgaca ttgaattcgt
     2641 catcggtgcg aatggctgcg aactgtgcag ggcgatctaa cgcggggtgc gtgccaaagg
     2701 tttcgtaatc gtgaaagagg aaggtaggtt gtttatctgt gtctgtcatt gtctagcatt
     2761 gtccactgta gttaattaac tgtctgttat aactcgaaaa gcaacccttt tttcacctga
     2821 gtatgtctac gaacgtcaaa tatagttgtt tactgtgcgc tgttgtccat gtgatattgt
     2881 gtaccagatg tgtgccaaac tattatttct gagcgtacca aatattattt atggctataa
     2941 gcgacacaaa actgcgtact atttatggta aaccatattc gggcccacaa gaagtggctg
     3001 atgccgatgg cctcagcgta cgaatttcac cgaagggggt catccagttc cagtaccgct
     3061 atcgctggca tggcaagcct aatcgacttg ggcttggtcg atacccatcc ctgtctttga
     3121 aggatgccag acagatcact gctgacttgc gaaagctcta tttctcagga acggatccac
     3181 gcacctattt tgaagagaag gtggagaact ccatgacggt cgcccagtgt ctcgactact
     3241 ggttcgacaa ctacgtctct acaactctca gagaaaagac ccaggcactt tatcgatcaa
     3301 cggttatgaa gcgcatgcat gacgccttcc ctaatcgtcc ggcatcttct atcacggtta
     3361 agcaatgggt tgacctgctt accgaagaag aaaaagataa tccacgccga gcaaggcagg
     3421 tgctaagtca actaagatca gcaattagtt ggtgcatgcg gcgtcagttg atagatagtt
     3481 gcgcaattat gagcatccag ccaagggact tcggctcccg cgctgaggta ggggatcggg
     3541 tactgtcgta tcacgaactg gctaagattt ggcttgctat tgaaagaagc cgtgcgtcta
     3601 cgtcaaataa gctccttcat cagatgctta tgctgtgggg ggcgaggctc tcagagctta
     3661 ggctggcaac aaagacagaa tttgacctgc tggacaacgt atggaccgta ccgaaagagc
     3721 atagcaagat gggtaatgtt atccgccgtc caatcttcga acaaa
//
LOCUS       DAZPDE010000252         3691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPDE010000252 DAZPDE010000000
VERSION     DAZPDE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.258"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHU0J_003857"
     CDS             108..2105
                     /locus_tag="ACHU0J_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR1218314.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACHU0J_003858"
     CDS             2168..3445
                     /locus_tag="ACHU0J_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR1218315.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          891 a          839 c          931 g         1030 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg t
//
LOCUS       DAZPDE010000253         3579 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPDE010000253 DAZPDE010000000
VERSION     DAZPDE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.259"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            49..1200
                     /gene="yiaY"
                     /locus_tag="ACHU0J_003859"
     CDS             49..1200
                     /gene="yiaY"
                     /locus_tag="ACHU0J_003859"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR1218316.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1365..2903
                     /gene="aldB"
                     /locus_tag="ACHU0J_003860"
     CDS             1365..2903
                     /gene="aldB"
                     /locus_tag="ACHU0J_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR1218317.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3122..3265)
                     /locus_tag="ACHU0J_003861"
     CDS             complement(3122..3265)
                     /locus_tag="ACHU0J_003861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218318.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3448..>3579
                     /locus_tag="ACHU0J_003862"
     CDS             3448..>3579
                     /locus_tag="ACHU0J_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1218319.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          890 a          858 c          939 g          892 t
ORIGIN      
        1 aatcctcagt aagctgccca ccctttttta cactttcagg agtgtgttat ggcatcttca
       61 actttcttta ttccttctgt gaatgtcatc ggcgctgatt cattgactga tgcaatgaat
      121 atgatggcag attatggatt tacccgtacc ttaattgtca ctgacaatat gttaacgaaa
      181 ttaggtatgg cgggtgatgt gcaaaaagca ctggaagaac gcaatatttt tagcgttatt
      241 tatgatggca cccaacctaa ccccaccacg gaaaacgtcg ccgcaggttt gaaattactt
      301 aaagaaaata attgcgatag cgtgatctcc ttaggcggtg gttctccaca cgactgcgca
      361 aaaggtattg cgctggtggc agccaatggc ggcgatattc gcgattacga aggcgttgac
      421 cgctctgcaa aaccgcagct gccgatgatc gccatcaata ccacggcggg tacggcctct
      481 gaaatgaccc gtttctgcat catcactgac gaagcgcgtc ataacaaaat ggcgattgtt
      541 gataagcacg tgactccgct gctttctgtc aatgactcct ctctgatgat tggtatgccg
      601 aagtcactga ccgccgcaac gggtatggat gccttaacgc acgctatcga agcgtatgtt
      661 tctattgccg ccacgccgat cactgacgct tgtgcactga aagccgtgac catgattgcc
      721 gaaaacctgc cgttagccgt tgaagatggc agtaatgcga aagcgcgtga agcaatggct
      781 tatgcccagt tccttgccgg tatggcgttc aataatgctt ctctgggtta tgttcatgcg
      841 atggcgcacc agctgggcgg tttctacaac ctgccacacg gtgtatgtaa cgccgttctg
      901 ctgccgcatg ttcaggtatt caacagcaaa gtcgccgctg cacgtctgcg tgactgtgcc
      961 gctgcaatgg gcgtgaacgt gacaggtaaa aacgacgcgg aaggtgctga agcctgcatt
     1021 aacgccatcc gtgaactggc gaagaaagtg gatatcccgg caggcctacg cgacctgaac
     1081 gtgaaagaag aagatttcgc ggttctggcg actaatgccc tgaaagatgc ctgtggcttt
     1141 actaacccga tccaggcaac tcacgaagaa attgtggtga tttatcgcgc cgcaatgtaa
     1201 tcatcattcc aacaacggct ggcaaattgt cagccgcttt ttcaactatc tctgtaatcc
     1261 ttgcccgtaa attcgtgata gctgtcgtaa agctgttacc gactggcgaa gatttcgcca
     1321 gtcacgtcta cccttgttat acctcacacc gcaaggagac gatcatgacc aataatcccc
     1381 cttcagcaca gattaagccc ggcgagtatg gtttccccat caagttaaaa gcccgctacg
     1441 acaactttat tggcggtgaa tgggtagccc ctgccgatgg tgagtattac cagaatctga
     1501 cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc gggcaaacga gacatcgatc
     1561 tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc gcacacctcg gtgcaggatc
     1621 gcgcggcgat tctgtttaag attgccgatc gaatggaaca aaacctcgag ctgttagcga
     1681 cagctgaaac ctgggataac ggcaaaccca ttcgcgaaac cagtgctgcg gatgtaccgc
     1741 tggcgattga ccatttccgc tatttcgcct cgtgtattcg ggcgcaggaa ggtgggatca
     1801 gtgaagttga tagcgaaacc gtggcctatc atttccatga accgttaggc gtggtggggc
     1861 agattatccc gtggaacttc ccgctgctga tggcgagctg gaaaatggct cccgcgctgg
     1921 cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac cccgctttct gtactgctgc
     1981 taatggaaat tgtcggtgat ttactgccgc cgggcgtggt gaacgtggtc aatggcgcag
     2041 gtggggaaat tggcgaatat ctggcgacct cgaaacgcat cgccaaagtg gcgtttaccg
     2101 gctcaacgga agtgggccaa caaattatgc aatacgcaac gcaaaacatt attccggtga
     2161 cgctggagtt gggcggtaag tcgccaaata tcttctttgc tgatgtgatg gatgaagaag
     2221 atgccttttt cgataaagcg ctggaaggct ttgcactgtt tgcctttaac cagggcgaag
     2281 tttgcacctg tccgagtcgt gccttagtgc aggagtcaat ctacgaacgc tttatggaac
     2341 gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc gctcgacagc gtgacgcaaa
     2401 tgggcgcgca ggtttctcac gggcaactgg aaaccatcct caactacatt gatatcggta
     2461 aaaaagaggg cgctgacgtg ctcacaggcg ggcggcgcaa gctgctggaa ggtgaactga
     2521 aagacggcta ctacctcgaa ccgacgattc tgtttggtca gaacaatatg cgggtgttcc
     2581 aggaggagat ttttggcccg gtgctggcgg tgaccacctt caaaacgatg gaagaagcgc
     2641 tggagctggc gaacgatacg caatatggcc tgggcgcggg cgtctggagc cgcaacggta
     2701 atctggccta taagatgggg cgcggcatac aggctgggcg cgtgtggacc aactgttatc
     2761 acgcttaccc ggcacatgct gcgtttggtg gctacaaaca atcaggtatc ggtcgcgaaa
     2821 cccacaagat gatgctggag cattaccagc aaaccaagtg cctgctggtg agctactcgg
     2881 ataaaccgtt ggggctgttc tgatataaga agctggtcgc attgggtatt cattgcctga
     2941 tgcgacgctt acgcgtctta tcatgcctac gggaacctga ccgtaggccg gataaggcgt
     3001 acacgccgca tccggcataa acaaagcgca cgttgttaca atttacgttt taaacctacg
     3061 tgcgcaataa cccagactac gccaacagaa gtgcgtttct ggggcgtgtt ttgtcaggag
     3121 tttaatctcg cgtttcgatg gtgagcgggc gaaccaggcc gtaaatcacc attggtgatc
     3181 cgaggccaat cagcaataaa cggtagataa ttgaaggttt aatacgctta atctgtactg
     3241 tggatgtttt actggagtta ttcattagtt tggccaatag ttccactatt tcaatatgat
     3301 aaacctcgtc ctgttgaaaa accagacagc acatttcaga aagtatataa aaaacaatat
     3361 gtcgttatat aaataaggaa attgcactgc atcatggcat ttcataatat gcgttaattg
     3421 ttctttttta tattctttga gggaaatatg ttcctggact attttgcact gggagtgctt
     3481 atttttgtat ttctggtgat attctatggg attattattt tacatgatat tccctacctg
     3541 attgctaaaa aacgtaatca tcctcatgcc gacgccatt
//
LOCUS       DAZPDE010000254         3559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPDE010000254 DAZPDE010000000
VERSION     DAZPDE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.260"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..649)
                     /locus_tag="ACHU0J_003863"
     CDS             complement(<1..649)
                     /locus_tag="ACHU0J_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1218320.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIY"
     gene            895..1656
                     /locus_tag="ACHU0J_003864"
                     /pseudo
     CDS             895..1656
                     /locus_tag="ACHU0J_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1763..2275)
                     /locus_tag="ACHU0J_003865"
     CDS             complement(1763..2275)
                     /locus_tag="ACHU0J_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218321.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2414..>3559)
                     /locus_tag="ACHU0J_003866"
     CDS             complement(2414..>3559)
                     /locus_tag="ACHU0J_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1218322.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1084 a          782 c          628 g         1065 t
ORIGIN      
        1 catatataaa atttgcacct ggcacacagg gtaaatgctt tttcgaaatt tcagcggcct
       61 ctatttgaga tttagcgatg ccaatctcgt taagattctc tgaaagatat aaatcacagg
      121 tgtcaatctt acctgattta accaatggat aatccgttat actgtaggta tctttaaaag
      181 ccacggcaaa catttcaata ataacaatta ctaccacaag cgaaatcaaa atgcgaaaaa
      241 gaggaatact ccttttgcgc tctacttttt caacaacatc ttcttgtaaa tgagaatcat
      301 cataagattg tttacttatt gttatgtttt cactgatgta aaagcctact ttgggtatcg
      361 taagaatcac attttcttca acaccaatat ttttaaaact tttgcgtaac agggatatat
      421 attggttcaa tgtgttgttt gacagatcaa atgaaaatct ctccaaaacg ctatcaatga
      481 ttgagttcct gtcaaccgct ccaccattac tttccagtaa cactgccaaa atacaccccg
      541 ccgtagctgg aaaatttatc tcatcctggg tatttacatt cgagagcgag catttgttcg
      601 gatcaaaaat aatcgcgtca ttgataatga aaaaacatct ccgctccatg caggcaacaa
      661 cacaaaataa gattttgtca ttttagccta ttcctggtgg aactgacaca agaaactaaa
      721 atacaaatat aaccctgaaa aagtcatata aaacttatat ccacagaata aaaaatcaaa
      781 catgtcagtt ttatcaatat aaagtatcgc aaggcgaaaa gtgatgcaag aatactccta
      841 agtaaatgaa agcgcaccac atgaaattat caacacatgt aatttcatca ttaggtgcta
      901 tcgaaaataa ctcaggagct acttaagatg aataatgtct tactcatgag acccgatgac
      961 tcagaattta tgatacctga tgactggtca gggctaaacg caaaaggcat tacatacgaa
     1021 caagataata gagtgacaga gtcttttctg ccttttaaat ctttttattg ttcaccggtg
     1081 tgcacgacag ttgaactcat ctcataaaat aattgttttg ctatgggtat ccgctacctt
     1141 ctggaaaaga aaagagactt aaggctaaat cactttaaag ccttctctca taaatgcgag
     1201 acaaaacctg acgtgttaat tatgaccggg tgcaccgtcg ctgatactta tagtatattc
     1261 tcacgcattg ttgaagaaaa tataatgaaa acgatgacac ctatcgtcat cattactggg
     1321 catcactcag aagatctttg ttctctggca tcgaaactgg gaggagttaa aataatatca
     1381 ccgacagaaa agattgcaaa aatagaaaac gctatcaatg atgcttttaa cagcaagcct
     1441 aaaaaaataa gatttttaac agatatactc tctcctcaac aaattgagac catgaaaatg
     1501 gttgcgcagg gtagatccgt taatgagatt tcagccgcaa tgaatattag catcaagacc
     1561 gtgtatgcac ataaagttcg tgctatcaat aagctgggaa ttaagaaaaa agtttatgaa
     1621 gcgctttttt ataaagcgct aattcagttc aactgatgta aaaatcaccc tcgcttaata
     1681 aataatatat tctatgtctt tttttataag ttaaaaaagg cgggatatca aatcccgcca
     1741 tgaaattaac taatcatctg cattattctg cgtagatata agcctgggta cggatggtgt
     1801 aagagtctgg ctcaacaacc tgttctttca tatgggaaac gacgcgatag gcaccacctg
     1861 ttccagtatt tttcagcatc atataacgct taccgccaat ctcttcggag gcagctgaag
     1921 gaggatcctg ctcaagcgtt gtcagggaaa ctttcagttt atggtccggg ttattgtcac
     1981 cagaaattac tgcatccgct gaagtactgc tctccgcata aatatcatcc gggaaagtta
     2041 cagcatagtc gtggctctgc ccatcagatg cgccaacaat acctgttgcc aaaggcgtcc
     2101 atgcagttaa cgtaccgcta tatacccctt tcactggttg aatggtatta ttaacaacga
     2161 taggcttggt caaagtaaga ttagctgacg cttcagtacc ctggcttact aaagttcccg
     2221 cctgagaagc aaaagctgtc cccgccagca tgattgctaa caatgttttc ttcattatat
     2281 tttctccatg attttcattt acactgcaaa atgcagtttc tttctttgca tttacgtgct
     2341 atcgcgaaaa tgcttccacc aaataatgga aacatcttca caaaaataaa ttatgagttt
     2401 cttaatttag aaactataaa catgtcgccg ctatctggta taaccctgat gcacttttct
     2461 tcgcaggaag ttggtatttt gcgatacagg attgatcttt agaataaccc catttcacat
     2521 taaactcgcc ttccgcactc aagccagaaa cataagcaat accatcttca cctacaatgc
     2581 cggccatatt ttcagtgtct ttgctataac taatcacagc gccgaagggg atgggtttac
     2641 cctctttctg ttttaaggtt actaatcctt tatccccgct cttcactgcc aatgtcgcag
     2701 gcacaacggc tccagctgtc ggtacaacca attgactagt ttgtggtaag tcgtaattct
     2761 tacctatcgt tttttcatcc agaataacat cgtctcttcg gtatggctgc actggcagta
     2821 ttgcataccc acgaaaatca gtactcagtc ccggccagcc attaacgtta acccccgttg
     2881 ctccgggtgc ctctataagg gctaatgtcc catccacatt ttgaccaaat gtaagtccat
     2941 cccttgtaat aagtatccca ccgcttaaat ttgcacctag ctgtttcatg ttttcgctgg
     3001 agctgtaatg gctactaaac tgaccatatg atccattttt gccaacttgc acaatgttca
     3061 tcgtgctctt cggttgtgac tgatgagcgt tagttatatt tgtcgctaca tcccaccata
     3121 caccaccact attctgacct gataatgaaa cctgatagtt attaccctgc tctcccgaac
     3181 gactcatctg tgaagacgca taaatttgat tttcgtcacc cagcgataat ggcactgaaa
     3241 tacccacgct taatttccga tctactttcc atttatgcga tgtcagcaag tcgttttttg
     3301 cccagttaat ggagaatgtt atgcctctcc ataccgtact ataactcagg ctggtagacc
     3361 gcgtgtttcc tgtgccccgg gaagaattat acctcgtggt attgaagttc aaattcccga
     3421 tattaccaag gggctgggat aaggatatcg tagagccatc gtgactattc ttatctcttt
     3481 ttctgttccg ttcatcctgt gcacttactg cttcagtcag tgtggcataa cctgatgcat
     3541 aacggttcgt ggttataga
//
LOCUS       DAZPDE010000255         3548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPDE010000255 DAZPDE010000000
VERSION     DAZPDE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.261"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHU0J_003867"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHU0J_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1218323.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHU0J_003868"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHU0J_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1218324.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHU0J_003869"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHU0J_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1218325.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZPDE010000256         3522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPDE010000256 DAZPDE010000000
VERSION     DAZPDE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.262"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..845
                     /gene="yibB"
                     /locus_tag="ACHU0J_003870"
     CDS             <1..845
                     /gene="yibB"
                     /locus_tag="ACHU0J_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR1218326.1"
                     /translation="TIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENE
                     MIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQL
                     KNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFP
                     FDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQK
                     ITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIF
                     LSRK"
     gene            1120..2316
                     /gene="kbl"
                     /locus_tag="ACHU0J_003871"
     CDS             1120..2316
                     /gene="kbl"
                     /locus_tag="ACHU0J_003871"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1218327.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2326..3351
                     /gene="tdh"
                     /locus_tag="ACHU0J_003872"
     CDS             2326..3351
                     /gene="tdh"
                     /locus_tag="ACHU0J_003872"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1218328.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          889 a          761 c          912 g          960 t
ORIGIN      
        1 ccacaattat cactgcatat tttgacattg gtagagggga ttggactgct aataaagggt
       61 tccgtgaaaa actagctcgt tcagttgatg tttattttag ttactttgag cgtctggccg
      121 cacttgaaaa tgagatgatt attttcactt ctcccgattt gaaaccaagg attgaagcga
      181 ttcgcaacgg aaaaccaaca acggttatcg ttatcgatat aaaaaagaaa tttagatata
      241 tcagaagtcg tatcgaaaaa attcaaaaag atgagtcatt tacaaataga cttgagcctc
      301 gacaattaaa aaacccagag tactggtcac cagagtatgt tttggtatgt aaccttaagg
      361 cctattttgt aaataaagct atcaacatgg gtttagtgaa aacgcctctt gttgcgtgga
      421 tagattttgg ctattgccgt aagccaaatg taactcgagg attgaaaatt tgggatttcc
      481 catttgatga aaataagatg catcttttca ctataaaaaa agggcttact gttacatctc
      541 aacaacaggc ttttgatttc atgattggca atcatgtata tattattggg ggcgcaattg
      601 ttggttcaca gcataaatgg aaagaattct ataaattagt tctggaaagt caaaaaataa
      661 cactaaacaa taacattgtg gacgatgacc agggcatttt tgtcatgtgc tattacaaac
      721 gttccgatct cttcaaccta aactatctgg ggagaggaaa atggttcgat ttgtttcgct
      781 gcttcaggag taacacttta ggggcaaaaa tgcaggcgct gagaattttc ctttcaagaa
      841 aataggtttc cctccattag tgggtagcgt tttgatgcca actgagatag gaaaaatgat
      901 gctttggcat cattttttct ttttatatca atgcagtaaa atcactcatc attatgctca
      961 gccaaaactg tacagttttt ctactatctg taggtgttga gagacatcaa gatacacgtt
     1021 atttatcctg aattttgcag aagtgttaac gcatcatctc gtcgcgacct ataagtttgg
     1081 gtaatatgtg ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaga attttatcag
     1141 cagttaacca acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt
     1201 attacgtctg cgcagcaagc agatatcacc gtggctgatg gaagccacgt cattaacttt
     1261 tgtgccaaca actatctcgg gctggcgaat catccggatc tgattgcggc agcaaaggcg
     1321 ggaatggatt ctcacggttt cggcatggct tcggtgcgtt ttatttgcgg cactcaggac
     1381 agccataaag agcttgaaca aaaactggcg gcattcctgg ggatggaaga tgcgattctc
     1441 tactcttcct gctttgatgc taacggtggc ctgtttgaaa cgctgctggg cgcggaagac
     1501 gccattatct ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa
     1561 gctaaacgct atcgctatgc caacaacgat atgcaggagc ttgaagctcg tctgaaagaa
     1621 gcgcgtgaag ccggtgcgcg tcatgtgctg attgccaccg atggtgtgtt ctcgatggac
     1681 ggcgtgattg ccaatctgaa gggcgtttgc gatctggcag ataaatacga tgccctggtg
     1741 atggtagacg actcccacgc ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa
     1801 tactgcgatg tgatggggcg tgtcgacatc atcaccggca cgctcggtaa agcgctgggc
     1861 ggggcttctg gtggttatac cgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt
     1921 tctcgtccgt acctgttctc caactcgctg gcaccagcaa ttgtcgccgc ttccatcaaa
     1981 gtgctggaga tggttgaagc gggtagcgag ttgcgtgacc gtctgtgggc gaacgcgcgt
     2041 cagttccgtg agcaaatgtc ggcggcgggc tttaccctgg cgggagccga tcacgccatt
     2101 attccggtca tgcttggtga tgcggtagtg gcgcagaaat ttgcccgaga gctgcaaaaa
     2161 gagggcattt acgtcaccgg tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt
     2221 cgtacccaga tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc
     2281 acgcgtattg gtaaacaact gggcgttatc gcctgaggat gtgagatgaa agcgttatcc
     2341 aaactgaaag cggaagaggg catctggatg accgacgtcc ctgtaccgga actcgggcat
     2401 aacgatctgc tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat
     2461 aactgggatg agtggtcgca aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat
     2521 gtcggtgaag tggtaggtat tggtcaggaa gtgaaaggct tcaaaatcgg cgatcgcgtt
     2581 tctggcgaag gccatatcac ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat
     2641 ctgtgccgca acaccattgg tgtcggtgtt aaccgtccgg gctgcttcgc cgaatatctg
     2701 gtgattccgg cgttcaacgc tttcaaaatc cccgacaata tctccgacga cctggcttcc
     2761 atttttgatc ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggtgggcgaa
     2821 gatgtgctgg tttctggtgc aggtccgata ggtattatgg ctgcggcggt ggcgaaacac
     2881 gttggtgcac gcaatgtggt gatcaccgat gttaacgaat accgccttga gctggcgcgc
     2941 aaaatgggta tcacccgtgc ggttaacgtc gcgaaagaaa accttaatga tgtgatggct
     3001 gaactgggca tgaccgaagg ctttgatgtc ggtctggaaa tgtccggcgc gccgccagcg
     3061 tttcgtacca tgctcgatac catgaaccac ggcggtcgta ttgcgatgct gggtattcca
     3121 ccgtctgata tgtctatcga ctggaccaaa gtgatcttta aaggcttgtt cattaaaggc
     3181 atttacggtc gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtctggc
     3241 ctcgatctct cgccgatcat tacccatcgt ttctctatcg atgatttcca gaagggcttt
     3301 gacgctatgc gttcgggcca gtccgggaaa gttattctga gctgggatta acacgaacaa
     3361 gggctggtat tccagccctt ttatctgagg ataatctgtt aattatgtaa aatcctgtca
     3421 gtgtaataaa gagttcgtaa ttgcgctgat cttttatata gctgttctca ttatctctct
     3481 accctgaagt gactctctca cctgtaaaaa taatatctca ca
//
LOCUS       DAZPDE010000257         3497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPDE010000257 DAZPDE010000000
VERSION     DAZPDE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.263"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHU0J_003873"
     CDS             <1..311
                     /locus_tag="ACHU0J_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR1218329.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHU0J_003874"
     CDS             468..1217
                     /locus_tag="ACHU0J_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1218330.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHU0J_003875"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHU0J_003875"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1218331.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHU0J_003876"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHU0J_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1218332.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3497
                     /locus_tag="ACHU0J_003877"
     CDS             3361..>3497
                     /locus_tag="ACHU0J_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1218333.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          864 g          764 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgttt
//
LOCUS       DAZPDE010000258         3489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPDE010000258 DAZPDE010000000
VERSION     DAZPDE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.264"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHU0J_003878"
     CDS             <1..77
                     /locus_tag="ACHU0J_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1218334.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHU0J_003879"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHU0J_003879"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1218335.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACHU0J_003880"
     CDS             1490..2614
                     /locus_tag="ACHU0J_003880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1218336.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHU0J_003881"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHU0J_003881"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1218337.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          920 a          760 c          850 g          959 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtata
//
LOCUS       DAZPDE010000259         3465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPDE010000259 DAZPDE010000000
VERSION     DAZPDE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.265"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..721)
                     /locus_tag="ACHU0J_003882"
     CDS             complement(<1..721)
                     /locus_tag="ACHU0J_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1218338.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
     gene            complement(844..1296)
                     /gene="tabA"
                     /locus_tag="ACHU0J_003883"
     CDS             complement(844..1296)
                     /gene="tabA"
                     /locus_tag="ACHU0J_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1218339.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1441..2034)
                     /locus_tag="ACHU0J_003884"
     CDS             complement(1441..2034)
                     /locus_tag="ACHU0J_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1218340.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2105..2818
                     /gene="bdcA"
                     /locus_tag="ACHU0J_003885"
     CDS             2105..2818
                     /gene="bdcA"
                     /locus_tag="ACHU0J_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1218341.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2950..3345
                     /locus_tag="ACHU0J_003886"
     CDS             2950..3345
                     /locus_tag="ACHU0J_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1218342.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
BASE COUNT          916 a          825 c          752 g          972 t
ORIGIN      
        1 tattcaatgc tttttctaat aaatccgtaa tcgtagaatc aactgaacct ttacaactca
       61 cctgcgagtt atttaactcc tgcgaattat ttaacttctg tgaattattt aactcctgcg
      121 aatcatttaa ctcctgtata ttatttaact tctgatgatt atttaataga cgcgctattt
      181 tcttgttagc ttcgcgttgg taacacgcca gaaccttgtt agtaatcagg tgtaatgaac
      241 tatcatcttt tctttcaaaa gacttccctt cataacgatt agtcgaacca tcagattgtt
      301 taaatataat gactgtatgt ccttcacgaa tccctttagt agaagctgag gcaagtgtaa
      361 tttctatctt caccttagat aaatccgctc gctccccttc aggattattc atgtaaaact
      421 tcattttttc attgccatca gtatctttag aataagatga gaactcaata tgagccatca
      481 tatttctacc cgcttcgttg gagcgtttgc taaaatccac ataacccagg gtaaatatct
      541 ttgccaaaaa ctccaaaaat gctctaggag aaagaatatt gcttctgcta ttctgccccc
      601 cggcaattcg ggcaaaatca ttcattccca cataccaggt atctaaggct tcattttttt
      661 gtttaaaatt gtcggctaat gtaatgtgtt gagaataatt caaatctgat attttgccca
      721 tgacgaactc cttgagttaa taagaacgtc taagcattac aaattttact cacaaaaaaa
      781 tatcaaaaaa cgctcttttc acaaactcgc cgtaaacctg gagttattta cggcgaaaag
      841 tcatcacgcc atcagcatct tcactactgc tttgcgaacc tgggctggcg cgcccactgc
      901 gcacagcggt ttatgcactt cccccggata aaacacaaca aaatcacctt catttaggat
      961 aactgttttc tcatcaacgc cttccggcaa aaatgcgatg tctttatcag ctaaccagtc
     1021 ggtatccggc gcgcctgcag gttgcgtgct gaaggtcatg ccttcctgac cttttaacac
     1081 aatctgaatg tcaagatagc gggcatggta ctccgcacgg cgagcttcgt acggctcggt
     1141 catatcttcc gagataagat aaaacagacg attgccttcg atatcatgct tgccctttgg
     1201 cgtttctgcc gtaacgtgtg ctttgatatg ctcaatcgcc tggcgtaact cctggggtag
     1261 ccacggctga agattatgaa tatttccgat gatcatcttt tccactcctt aattaaaaca
     1321 atgtttcatt tctatcgtta tacgaaaaga tgatcaattc cgccagcctt aacgcgtgaa
     1381 ttattgtctt atcacaactt atcggcaggc gcgttcccgc ctttcggatc aaggccagca
     1441 tcactccttg agaagagttt ttatcgcttc ccctgccagg gcagctgttg cacagagttg
     1501 ttcgatggag tgcccctccc gtgccttcgc agaaagccct gacatcacgg tactcataaa
     1561 atcagtcaca cattgtgcgc tttgtggatg ccgcctggcg atatagtcat aaatggtcgt
     1621 ttctgcggcg tgataatatt gaatggcaat atcacgcgct tgtggatcat gactatgaat
     1681 accttcaaga accatacagc cagcgcagcc gccgttttgg ctatatctgc gcgccgcttc
     1741 ttttaatacc tcaaccaggc actcgcctac cggacgatca tcacgaagaa tatcggcaag
     1801 cggaatagct tccgtaccga catattcatt gagtacacgg ctaaataacc cagccttact
     1861 gccaaaagcc gcgtagagac tcggggggtt aataccaaga taatcagtaa cttcagagac
     1921 acttactgca tcgaaacctt tttggtgaaa aagcactttt gccgcagaga ctgcctgctc
     1981 aggagcgaaa cgtctgggac gacctggaac gcgagattgt tttttagtga ccattacaaa
     2041 acctgttgac agaaagttaa aacagttttg taatgcatgt tacataataa atcaaggagt
     2101 ccttatgggc gcttttaccg gtaagacagt tctcatcctc ggtggcagtc gtggaatcgg
     2161 tgccgctatc gtacgtcgtt tcgtcaccga tggggccaat gtacgattca cctatgcggg
     2221 gtcgaaagat gccgctgaac gtctggcaca agagactgga gcgacagcag tattcacaga
     2281 tagtgctgac agagacgctg ttattgatgt cgttcgtaag agcggcgcat tggatatcct
     2341 ggtggtaaat gcaggtattg gcgtctttgg cgatgccctg gaattaaatg ccgacgatat
     2401 tgatcgcctt ttcaaaatca atattcatgc tccttatcat gcctctgttg aagccgcccg
     2461 gcagatgccc gaaggcgggc gcatcttaat catcggctcc gtgaatggcg atcgtatgcc
     2521 tgttgcaggc atggctgctt atgccgccag taaatctgcc ctgcaaggca tggcgcgcgg
     2581 gctggcccgt gattttggac cgcgagggat caccatcaac gtcgtccagc caggaccaat
     2641 tgataccgac gttaatcccg ccaacgggcc aatgcgcgat atgttgcata gttttatggc
     2701 catcaaaaga catgggcaac cggaagaggt cgctggtatg gtcgcatggt tagcagggcc
     2761 agaagccagt tttgttaccg gcgcgatgca taccattgat ggcgcgtttg gcgcataacc
     2821 gactacgctc aattaagccc agccattatc catgatgtct gggttttgtt tactaacgtc
     2881 gtccccctaa aagcggctcc cgataaatat aaatcttctg ttcatgtcgt aaatcttgag
     2941 gataaccaaa tggtagaaag aaccgctgtt ttccctgctg gccgacattc actgtatgct
     3001 gagcatcgtt attctgcggc tattcgttcc ggcgatttgc tgtttgtttc cgggcaagta
     3061 ggaagtcgag aggacggaac accagaaccc gattttcagc aacaagtcag actggccttt
     3121 gataatttgc atgcgaccct ggcagctgcg ggatgcactt ttgacgatat cattgatgtt
     3181 acgagctttc ataccgatcc ggaaaaacaa tctgaagaca tcatgacggt gaaaaatgaa
     3241 atatttagcg ccccacctta tccaaactgg acggcggtgg gtgttacctg gctggcaggc
     3301 tttgattttg aaattaaagt gatagcgcgt atccctgagc tataagcaat agtgttagcc
     3361 gttcgttttc accctccgcc ctataagtcg gatgaatgga ataaaatgca tttctgattg
     3421 cgtgaaaatg aaaaagaaaa aagcaggaaa tgtctgcaat tattg
//
LOCUS       DAZPDE010000260         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPDE010000260 DAZPDE010000000
VERSION     DAZPDE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.266"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHU0J_003887"
     CDS             complement(226..429)
                     /locus_tag="ACHU0J_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218343.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHU0J_003888"
     CDS             complement(431..586)
                     /locus_tag="ACHU0J_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1218344.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHU0J_003889"
     CDS             complement(789..1196)
                     /locus_tag="ACHU0J_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218345.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHU0J_003890"
     CDS             1273..1500
                     /locus_tag="ACHU0J_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1218346.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHU0J_003891"
     CDS             1484..1906
                     /locus_tag="ACHU0J_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1218347.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHU0J_003892"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHU0J_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHU0J_003893"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHU0J_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZPDE010000261         3449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPDE010000261 DAZPDE010000000
VERSION     DAZPDE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.267"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            111..827
                     /gene="nanC"
                     /locus_tag="ACHU0J_003894"
     CDS             111..827
                     /gene="nanC"
                     /locus_tag="ACHU0J_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1218348.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            847..1953
                     /gene="nanM"
                     /locus_tag="ACHU0J_003895"
     CDS             847..1953
                     /gene="nanM"
                     /locus_tag="ACHU0J_003895"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1218349.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2018..2998
                     /locus_tag="ACHU0J_003896"
     CDS             2018..2998
                     /locus_tag="ACHU0J_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1218350.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3108..3313
                     /locus_tag="ACHU0J_003897"
                     /pseudo
     CDS             3108..3313
                     /locus_tag="ACHU0J_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1010 a          735 c          807 g          897 t
ORIGIN      
        1 ccgttgccaa agacactata agcctggcaa aaaaatatta cacaacataa atgctaattg
       61 tttatgcggg ctttgtattg ctttctgtat cctacaaatg agtgaaattt atgaaaaagg
      121 ctaaaatact ttctggcgta ttattactgt gcttttcgtc cccattaatt tctcaggctg
      181 cgacactgga cgtacgtggt ggatatcgta gtggaagcca cgcctatgag actcgactca
      241 aagtcagtga gggatggcaa aatggatggt gggcaagcat ggaaagtaat acctggaata
      301 ccattcatga taataaaaag gaaaatgccg cactcaatga tgttcaggtt gaagttaatt
      361 acgcaattaa acttgatgat caatggacgg tgcgcccggg aatgttaacg cattttagca
      421 gcaacggcac acgctacgga ccctacgtaa aactgtcctg ggacgcgaca aaagatctta
      481 attttggcat tcgctatcgt tacgactgga aagcttaccg acaacaagac ttatccggtg
      541 atatgtctcg tgataacgtt catcgttggg atggatatgt cacttaccat attaatagtg
      601 atttcacctt cgcatggcaa acgacgctat acagcaaaca gaatgattat cgctatgcaa
      661 accacaagaa atgggcgacg gaaaatgcct ttgttctaca ataccatatg acgcccgata
      721 ttacgccata catagaatat gactaccttg accgccaggg tgtttacaac ggcagagata
      781 atttatcgga aaacagttat cgcattggtg tgtcatttaa actgtagtag acaggagaca
      841 gtcacaatga ataaaactat aatggcgctt gctatcatga tggcttcatt tgccgcaaac
      901 gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg gtaccggagc aattgataac
      961 gacactgtct acattggttt aggtagcgca ggtacagcat ggtacaagct agatacacag
     1021 gccaaagata aaaaatggac tgcgttagct gcattccctg gtggaccgag agagcaagca
     1081 acctctgcat ttattgatgg caatctgtat gtgtttggcg gcattggcaa aaacagcgag
     1141 ggattgactc aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa tagctgggtt
     1201 aaattgatgt cgcacgcgcc gatgggcatg gcgggtcatg tgacttttgt acacaacggc
     1261 aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt tgaagatctc
     1321 aacgaggctg gaaaagattc aaccgctata gataaaatca acgcccacta ttttgacaaa
     1381 aaagcagaag attatttctt caataagttt ctgttgtctt ttgatccctc aacacagcaa
     1441 tggagttacg ctggcgaatc gccctggtac ggaacggctg gtgcggcggt tgtgaataaa
     1501 ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac ggatgccgta
     1561 tttgaacttg atttcaccgg taataattta aaatggaata agcttgatcc cgtctcatca
     1621 ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg attctcttat atttgctgga
     1681 ggggccggat tcaaaggttc acgagaaaat taccagaacg gtaagaacta tgcgcatgaa
     1741 ggcctgaaaa aatcatatag cactgatatt catctttggc ataacgggaa atgggataaa
     1801 tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat tgccctggaa taatagttta
     1861 ttaattattg gcggtgaaac tgcaggcggc aaagcggtga cggattcagt tttaatttct
     1921 gtgaaggata ataaagtcac agtacaaaac taacgcttca gggccccggt aaaggggccc
     1981 tgctatcaat ttgtcatttg tattacgcga aaattatatg aacgcaataa tatcacccga
     2041 ttattactat gttcttaccg ttgctggtca gtctaatgcc atggcgtatg gcgaaggact
     2101 gccattaccg gacagggaag atgcgcctca ttccagaatt aaacaattag cgagatttgc
     2161 gcatacgcat cccggaggcc cgccatgtca ctttaacgac attattccac tgactcactg
     2221 cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacga atcatcaaac
     2281 acagtacggc accgttggcc aggcactgca tattgcacgg aaattactgc ccttcattcc
     2341 tgataatgca gggattctta tcgttccgtg ttgccgtggc ggatcggctt ttaccgcggg
     2401 cagcgaaggg acatattcag aacggcacgg agccagccat gatgcttgtc gttggggaac
     2461 ggatactccg ctgtaccagg atttagtcag cagaacgcga gccgcactgg caaaaaatcc
     2521 gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa tgaccagtga
     2581 ctacgcgtca caccctcaac actttaatca tatggttgaa gcctttcgta gggatctaaa
     2641 acaataccat tctcagctta ataatattac tgacgcgccg tggttttgcg gcgataccac
     2701 ctggtactgg aaagaaaatt tcccgcattc gtatgaagct atttatggca attatcaaaa
     2761 taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa gaggactgac
     2821 gaacgcgcct gatgaagacc cggacgattt aagcacggga tattacggtt cagcgtaccg
     2881 gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcacgg cagcccgtcg
     2941 ggggattatt tctgacagat ttgtagaagc aattttgcag ttttggcgcg aaaggtgagc
     3001 ggggagttta ttcatggggg atgtccttgt tcgttctcag agtagacaac aaaaaacaaa
     3061 tgagatcaag gtaatatctc ttcacttgcg ggctcgaaca gcaggcaatt gccaatataa
     3121 taatctggca caagggtgaa aaacgcgcac cgcataaacc gttgttattg cttacagatt
     3181 agccggatgc cttaatagac atccgcgcct tttcgattat ggctcggaaa tctacgaaca
     3241 tctacacagt ttactggaac gttttgggcc acagcgttcg cagcaccgtc ctgatatgcc
     3301 gttctggcga ttataagtcg acggattctg gcaaatagac gatgatgata tgcttaatga
     3361 tgttcaaaga aattaggcca ttattatatt attgcttgca ttttctttca ttcctaaaag
     3421 cacccgccat taatcccagg cgcattatg
//
LOCUS       DAZPDE010000262         3417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPDE010000262 DAZPDE010000000
VERSION     DAZPDE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.268"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHU0J_003898"
     CDS             <1..239
                     /locus_tag="ACHU0J_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR1218351.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="ACHU0J_003899"
     CDS             249..887
                     /locus_tag="ACHU0J_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1218352.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="ACHU0J_003900"
     CDS             890..2053
                     /locus_tag="ACHU0J_003900"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1218353.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..>3417
                     /locus_tag="ACHU0J_003901"
     CDS             2137..>3417
                     /locus_tag="ACHU0J_003901"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1218354.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGS"
BASE COUNT          760 a          806 c          987 g          864 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttcc
//
LOCUS       DAZPDE010000263         3369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPDE010000263 DAZPDE010000000
VERSION     DAZPDE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.269"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHU0J_003902"
     tRNA            41..116
                     /locus_tag="ACHU0J_003902"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Glu,seq:ttc)
     gene            310..3213
                     /locus_tag="ACHU0J_003903"
     rRNA            310..3213
                     /locus_tag="ACHU0J_003903"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          896 a          743 c         1030 g          700 t
ORIGIN      
        1 gaaagtgaaa agcaaggcgt cttgcgaagc agactgatac gtccccttcg tctagaggcc
       61 caggacaccg ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg
      121 ctggtttgtg agtgaaagtc acctgccttg atatctcaaa actcatcttc gggtgatgtt
      181 tgagatattt gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgaa
      241 agttgttcgt gagtctctca aattttcgca acacgatgat gagtcgaaag aaacatcttc
      301 gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg
      361 aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga
      421 tttccgaatg gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt
      481 aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga
      541 gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg
      601 ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc
      661 acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg
      721 accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa
      781 aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc
      841 agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat
      901 attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg
      961 ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg
     1021 ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg
     1081 ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg
     1141 tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg
     1201 acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc
     1261 gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag
     1321 tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag
     1381 cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg
     1441 taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg
     1501 ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta tcagaagtgc
     1561 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
     1621 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
     1681 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
     1741 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
     1801 gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca
     1861 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
     1921 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
     1981 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg
     2041 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
     2101 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
     2161 taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
     2221 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
     2281 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
     2341 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
     2401 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
     2461 gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
     2521 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
     2581 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
     2641 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
     2701 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     2761 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     2821 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     2881 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
     2941 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
     3001 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
     3061 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
     3121 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
     3181 aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt ttggcggatt
     3241 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     3301 agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     3361 tgaaacgcc
//
LOCUS       DAZPDE010000264         3348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPDE010000264 DAZPDE010000000
VERSION     DAZPDE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.270"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHU0J_003904"
     CDS             19..897
                     /locus_tag="ACHU0J_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1218355.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHU0J_003905"
     CDS             894..2285
                     /locus_tag="ACHU0J_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1218356.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHU0J_003906"
     CDS             2282..2539
                     /locus_tag="ACHU0J_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218357.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHU0J_003907"
     CDS             2634..2834
                     /locus_tag="ACHU0J_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR1218358.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHU0J_003908"
     CDS             2827..3207
                     /locus_tag="ACHU0J_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1218359.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZPDE010000265         3325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPDE010000265 DAZPDE010000000
VERSION     DAZPDE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.271"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHU0J_003909"
     CDS             <1..208
                     /locus_tag="ACHU0J_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218360.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHU0J_003910"
     CDS             269..457
                     /locus_tag="ACHU0J_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218361.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHU0J_003911"
     CDS             454..1281
                     /locus_tag="ACHU0J_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1218362.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHU0J_003912"
     CDS             1325..2074
                     /locus_tag="ACHU0J_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1218363.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHU0J_003913"
     CDS             2071..2640
                     /locus_tag="ACHU0J_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218364.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHU0J_003914"
     CDS             2637..2759
                     /locus_tag="ACHU0J_003914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218365.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHU0J_003915"
     CDS             2764..3006
                     /locus_tag="ACHU0J_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1218366.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHU0J_003916"
     CDS             3010..3156
                     /locus_tag="ACHU0J_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218367.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHU0J_003917"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHU0J_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR1218368.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          854 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZPDE010000266         3324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPDE010000266 DAZPDE010000000
VERSION     DAZPDE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.272"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHU0J_003918"
     CDS             complement(<1..171)
                     /locus_tag="ACHU0J_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR1218369.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHU0J_003919"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHU0J_003919"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1218370.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHU0J_003920"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHU0J_003920"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1218371.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHU0J_003921"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHU0J_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR1218372.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2840..3175)
                     /locus_tag="ACHU0J_003922"
     CDS             complement(2840..3175)
                     /locus_tag="ACHU0J_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1218373.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          700 a          853 c          936 g          835 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
     2761 gcaagcgtcg catcaggcag tcggcatcgt tgccggatgc ggcgcgagcg ccttatccgg
     2821 cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt ttcatcggac
     2881 caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg atgtttttca
     2941 ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct ttaatgatgg
     3001 taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc agctcgtcgc
     3061 tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag atgtacatgc
     3121 cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat gacatgtgga
     3181 tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg aaaaaggccg
     3241 caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga cgacgatcgt
     3301 tttcatttga aaggaaacgc aaaa
//
LOCUS       DAZPDE010000267         3278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPDE010000267 DAZPDE010000000
VERSION     DAZPDE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.273"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..261
                     /gene="yobA"
                     /locus_tag="ACHU0J_003923"
     CDS             <1..261
                     /gene="yobA"
                     /locus_tag="ACHU0J_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1218374.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            265..1137
                     /gene="copD"
                     /locus_tag="ACHU0J_003924"
                     /pseudo
     CDS             265..1137
                     /gene="copD"
                     /locus_tag="ACHU0J_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1150..1491
                     /locus_tag="ACHU0J_003925"
     CDS             1150..1491
                     /locus_tag="ACHU0J_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1218375.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1884..2961)
                     /locus_tag="ACHU0J_003926"
                     /pseudo
     CDS             complement(1884..2961)
                     /locus_tag="ACHU0J_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2927..3208)
                     /locus_tag="ACHU0J_003927"
     CDS             complement(2927..3208)
                     /locus_tag="ACHU0J_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1218376.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT          782 a          749 c          751 g          996 t
ORIGIN      
        1 gcgcaagtga cagctgcccc gcaggcaatc actttaaact tctcggaagg cgttgaaaca
       61 ggattcagtg gtgcaaaaat cacggggcca aaaaacgaaa atattaaaac attaccagcg
      121 aagcggaatg agcaggacca aaagcaactg attgttccac tggccgattc gctgaaaccc
      181 ggaacctaca ccgttgactg gcatgttgtt tcggtggacg ggcataaaac gaaaggacac
      241 tacaccttta gcgtgaaata aatcatgctg gcgtttacct ggatcgcgct gcgatttatc
      301 catttcacct cgttgatgct ggtctttggc tttgcaatgt atggtgcctg gctggccccc
      361 ttaatgattc gtcgcttgct gacgaagcgt tctctgcgtt tacaacagca cgctgccgtc
      421 tggagtttga tcagtgccac tgcaatgctc gccgttcagg gcgggttgat gggaacggga
      481 tggacagatg tattttcgcc aaacatttgg caggctgttt tgcaaacgca gtttggtggc
      541 gtctggttat ggcaaattgt tctcgccctc gtaacgttga tcgtcgccct tatgcaaccg
      601 cggaatatgc cacgtttgct gtttatgctc actaccgcgc aatttattct gctggcggga
      661 gtggggtatg cgacgctgaa tgaaggggta acagcgaaaa tccatcagac taatcacgcg
      721 attcacctga tttgtgcggc ggcgtggttt gggggattac tgccggtgct ctggtgtatg
      781 caactcatca aaggccgttg gcgacactag gctattcagg cgctgatgcg tttttcctgg
      841 tgcgggcatt ttgcggtgat cggcgtactg gcaagcggcg tgcttaatgc attgttgatt
      901 accggatttc cccccacgct tacgacttac tggggccaac tcttattgct caaagccatc
      961 ctggtgatga ttatggtggt cattgcgctg gctaatcgtt atgttcttgt accgcgcatg
     1021 aggcaggatg aagatcgtgc ggcaccctgg tttgtgtgga tgactaaact cgaatgggcg
     1081 ataggtgcgg tagtgctggt gatcatcagc ctgcttgcga ctctcgaacc tttttgatgg
     1141 attacgaaaa tgatgaaaaa aagtattctg gcgtttctgt tactcaccag ttctgcagcg
     1201 gcgctggcgg ctccacaggt gataaccgtg agtcgttttg aagtgggtaa agacaaatgg
     1261 gcgtttaatc gcgaagaggt aatgctgact tgccgaccgg gtaatgcttt gtatgtcatc
     1321 aacccaagta ccctcgtgca gtatccttta aacgatatcg cacaaaagga agttgccagt
     1381 gggaagacca atgcccaacc catttcggtg attcagattg atgatcctaa caatcccggc
     1441 gaaaaaatga gtctggcacc gtttatagaa cgagctgaaa aactctgtta attacctaaa
     1501 atagcctttt gatttccaat aaaaaaaccg cctcagttct ttcaccagaa cgggcggttt
     1561 ttaacattta agctgatgag caccgcgctt tttattgacc attttgcacg caaactggaa
     1621 aacctggcgt cgtcatctat tcttaaaggg caaggcaact aagcctgcat taatgccaac
     1681 ttttagcgca cggctctctc ccaagagcca tttccctgga ccgaatacag gaatcgtatt
     1741 cggtcttttt ttaattgtat ttaaaatcaa ttagttgcaa acgtctcccc gaaattcccc
     1801 gaaatttact cgaatttctg tattccggtc ttttttggtt atatcacaac caaaatacat
     1861 ttaacaatcc atttacgtta aaatcagagc agtaagtacg ttttttctct ctcatcaaga
     1921 tacatttttg ttgtcttctc cgatgtgtgg ccaagtagac gctgagcaaa ttcttctcca
     1981 catatttctt tgtacaatcg tccagccagg cttctgattt cgtgaaaagt tggtgggttt
     2041 tcactgaaca gtgatacctg ttaattttct ggctgcgaca aatttctttg tcaggccgtc
     2101 cgggtgaatg ctgccgtcag ggctgttttt tctaatcccg gcactgatta gataatcccc
     2161 cctacttacc atgcggcact gttcaactac tgcaccaagt cgtaaaccag cgacaggaag
     2221 gctgagtgac agggggatag caatcatcat tcctgtctta atttgcctga tgtggagacg
     2281 atcatcataa atatcactga accgcatatt cgttatgtct tcgcgacgtt gtcctgttac
     2341 aagggctaag tccatagcta atgggaacca tgccggaagt tgatctgctg cctccctgat
     2401 gcagttgtat gtctttagtt tcagccgttc tcttgtaact actattttcg gtgctcttgt
     2461 tggcgttact ggattttgag atatacgtcc ttcaacaatg gcttcgcgga acatatcaga
     2521 taacacagaa cgcattgatc ctgccatcgt gtttttccct ccttcaatct acaaatcaag
     2581 aaactcggcg atatggcgag tggttatttc tgtcagtaaa acccctccta atttttcttt
     2641 tattgtctcc agttgattta cccgagtttt ataagtattt ctggacgctt ttctccttat
     2701 aagaatcgtt ttgtaacgtt caatccagcc tgccatagta aatgagtcga cccctttaag
     2761 cttttcaatt agggcagcag gagagtagtt tttgtatata taatgatttg cttcaattgc
     2821 ctgcgctact gcatctcttc ttgaaatttt acctagtgta aattcttctt tcgtcagagg
     2881 gttgcgccag taatatgctt tgtctctcct tcgatatgtt aagtttctag gcaaattggg
     2941 atcgtatttt ttccgctgca tgttttaact tctccattaa cggactgtct ctcccttgtc
     3001 gcccattagg ctgatggtgt gttatatcgg tatcaacctt atttgggttg atatagaaag
     3061 cctccggaac caccctgtaa ctcctcccgt gtagttcagg tgcaggataa atgtttccat
     3121 tccttgccca tcgtctcagc gttgatattg atggtgggtt atccggatat ctgagttttc
     3181 cccacgtttt gagtgtcaca agattcattg ccatacctct cacgatatga ccgccagtaa
     3241 atatacagaa tactggcggg tgtggttgat ttttaata
//
LOCUS       DAZPDE010000268         3236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPDE010000268 DAZPDE010000000
VERSION     DAZPDE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.274"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHU0J_003928"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHU0J_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1218377.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHU0J_003929"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHU0J_003929"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1218378.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHU0J_003930"
     CDS             1252..1521
                     /locus_tag="ACHU0J_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1218379.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHU0J_003931"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHU0J_003931"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1218380.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHU0J_003932"
     CDS             2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHU0J_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1218381.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
BASE COUNT          820 a          775 c          792 g          849 t
ORIGIN      
        1 aaaggctatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgccgg gtgcgacgct
       61 ggctgcgtct gatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaaatgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgta
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cccggacgga tccctctggc gcataatgcc gggatcagtt
      541 ttttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaacaa atgcatcttt
      661 atgatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaata gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcatggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gcggctaaat catggggaat gtaaggcgta tcgcacggac aaaacaaaaa
      901 ccactcacca gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcctgcctga tagatttcct gatgacgatt
     1021 agcattaacc acgacgtgag agagctgcgt cttaagcgcg tcagcgacat gttgccacag
     1081 cggtttgcca tttaagtcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt cattagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacttt
     1321 aacttgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggcgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga cgctctgttt aagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 gcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagctggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt attcatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agcctcgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcatttc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agcccagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcatt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctac gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaatg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagcc gcagctgatg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgatacg gtgaaatatc tgtccgagga aaaataattc aatgta
//
LOCUS       DAZPDE010000269         3188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPDE010000269 DAZPDE010000000
VERSION     DAZPDE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.275"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="ACHU0J_003933"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="ACHU0J_003933"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1218382.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="ACHU0J_003934"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="ACHU0J_003934"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1218383.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="ACHU0J_003935"
     tRNA            2651..2726
                     /locus_tag="ACHU0J_003935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>3188)
                     /locus_tag="ACHU0J_003936"
     CDS             complement(2741..>3188)
                     /locus_tag="ACHU0J_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1218384.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          877 g          752 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     2761 ccttatttct tgactgggac aaatttggga ccgatgggtt cagaatcaag tctatttgcc
     2821 gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata tcgacgaacc atttcgatag
     2881 actcccagcc tcccatttcc tgtaacactg ataacgggac tccggcttga accagccaac
     2941 ttgcccaggt gtgtctcaag tcgtgaaatc tgaaatcatc aataccagcc cgtctcagcg
     3001 ccgctttcca ggctgtgttt gcgtcatacc gcatcttcct tactgttggc gctttcgttc
     3061 cgtctggttt ggtacagctt tccttgtaca caaataccca acggtgatga tttccgattt
     3121 gttttttcaa tacgcgacat gcagtatcat tcagcgcaac gccaattgcg cggtttgatt
     3181 tactctct
//
LOCUS       DAZPDE010000270         3179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPDE010000270 DAZPDE010000000
VERSION     DAZPDE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.276"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU0J_003937"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU0J_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1218385.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHU0J_003938"
     CDS             complement(1772..1906)
                     /locus_tag="ACHU0J_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1218386.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHU0J_003939"
     CDS             complement(2552..2722)
                     /locus_tag="ACHU0J_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR1218387.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZPDE010000271         3147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPDE010000271 DAZPDE010000000
VERSION     DAZPDE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.277"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            55..749
                     /locus_tag="ACHU0J_003940"
     CDS             join(55..301,301..749)
                     /locus_tag="ACHU0J_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1218388.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(868..1266)
                     /locus_tag="ACHU0J_003941"
     CDS             complement(868..1266)
                     /locus_tag="ACHU0J_003941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218389.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1263..1676)
                     /locus_tag="ACHU0J_003942"
     CDS             complement(1263..1676)
                     /locus_tag="ACHU0J_003942"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR1218390.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            complement(1710..>3147)
                     /gene="gadC"
                     /locus_tag="ACHU0J_003943"
     CDS             complement(1710..>3147)
                     /gene="gadC"
                     /locus_tag="ACHU0J_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR1218391.1"
                     /translation="KSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIM
                     FLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGA
                     VSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTI
                     IPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAV
                     HVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFE
                     WAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHH
                     LMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDT
                     KRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPI
                     LIYAFRKSDWKDPNSDFEPFSWEKRTSN"
BASE COUNT          990 a          664 c          628 g          865 t
ORIGIN      
        1 gatgcctcta attagttgaa tctgatgtat aatacgggct tttgaggtta tctcatgacc
       61 agcgttaata ttcattgtcc ccgttgtcag tcagctcagg tttaccgcca tggtcagaac
      121 cctaaaggcc gtgacagact tcgctgccgt gactgccacc gtgtgtttca gttcacttat
      181 acttatcaag cacgtaagcc gggtatgaaa gagctgataa ctgaaatggc ctttaatggt
      241 gccggggttc gcgataccgc caggacactg aaaattggta gtaacaccgt catccggact
      301 ttaaaaaact cgcgccaaag cgaataactt cttcgcctgt tgcccatgct gatgtggcgt
      361 ttatctgcga gcttgatgag caatggagct acgttggcag taaagcccgg caacattggc
      421 tcgggtacgc gtacaacacc aaaacaggcg gtgtactggc ctacactttt ggtccccgaa
      481 ccgatcaaac gtgccgggag ctactggcac tgcttacacc cttcaacatc ggcatgctga
      541 ccagcgatga ctggggcagc tatggccggg aggtgccgaa gaataagcat ctgaccggca
      601 aaatattcac ccaacgcatt gagcgtaata atctgacgct acgcacccgc attaagcggt
      661 tgggtcgtaa aacaatctgc ttctcacgct cagttgagat ccacgaaaaa gtgatcggag
      721 cgtttatcga aaaacacatg ttctactaat tggatgcact acccagtatg taactgggtt
      781 tgtccataaa atgcacctta ctcagttggt gtccaacttt tggggtgcag ttcagaagga
      841 cgggatttta cggtgtaccg gataaattta aaactgcctg atactgaatg tatgaggttt
      901 cgtaatgctc aattccccta gtaccgtttt gtagatctcc atacttccac tagtatttat
      961 ttgcaaagtt cctatatttg aggagtttaa taaatttagc tgtcccaagt gatttttaat
     1021 agagatagcc tgatcatgtg caggattaat aaattttata tccggtgcaa ctgcttcaaa
     1081 aaaattctga acaattggat agtgagtaca gccgagaata atttcattta ctggatggag
     1141 ggaaattaaa tggttaacat gttttttgat gtcattcaag attgcaggag cattaagatt
     1201 accttcctca attaacattg caagattttt gcttggttct ccaaatacat ttattgttga
     1261 attcaatttt ttattagctc tttgtgacac cccgttttaa tagtaaactc ggtagcgata
     1321 ataccaacat ttttatattg ctggcgaatc acatcttcta ctgcaggttc aatgatggat
     1381 aagataggaa acggaaagct cttagcatat ccgctgtatt cttcggattc caaagttgag
     1441 gatatagtat tacatgcgac agcaactagt ttgacattcc ttgactgtaa aaaagtcagc
     1501 atttttttag tcaaactgat gatatcatcg gcagaacggt tgccataagg tgcattcata
     1561 ttatctccac aatagataat atcttcacca ggaagcaaat taattatttc ctttaccgat
     1621 gtcaatccac cgacacctga gtctattaca ccaattggag attcagcact aaacatattt
     1681 aacctcatct tttgtagctt atggaaaatt taattactag ttcttttttc ccaagaaaat
     1741 ggttcgaaat cagagtttgg atctttccag tcagattttc ggaaggcgta aattaatatt
     1801 ggaattaata aaaatagggc aactaatact aacaaataaa gaacatagct ggttggacta
     1861 ccagttggga tttgctcggg cggaataaaa cttaatacaa aagccagaaa agaccctaag
     1921 aatccaagtg ttgcccagaa aaataaagca ggggctcgat atgggcgttt tgtatcaggt
     1981 tgagaatgtc gtagatagat agcactagcg aacatgagca tatacataac taaatagagc
     2041 ataactgtta actgtgataa aatctgaaaa gctgcttgaa ctgaaggcat tatcacaaaa
     2101 gtaacagaaa gaactgagac aattatacct tgtacaaaca ttagatggtg ccccattcca
     2161 tgacgattgg ttttttggaa aaacttaggt aaataaccag ctttcgcgat cgctaataca
     2221 cctgtattag gccctgcaat ccaagtaacc accccaccca gtacgccaat tgctagcata
     2281 aatgccatca cagagccaag ccatggcact ccagcccatt cgaacagcat atcgtaggca
     2341 accaacaaac tttgagttag actaatttta tctgtaggaa taataaaggc gacccctaaa
     2401 gtactgagca ggaatattgc aatagtacct atagaagcga tggtaattgc aattgggtat
     2461 tttttagttg gattatctaa atcgttaaca tggaccgcat tcatttccat ccctgcataa
     2521 gcaagaaata tgctggcggc aagaacaata ttctggaagt tagctatatt agggaaaaga
     2581 tcaccccaac ctaatgggat caccgctggt ttaccgctga accacagata ggagaaacca
     2641 agaataacaa ggataatagc tggaataatt gtccctataa taccgcacca cttagatacc
     2701 tgcgagaaaa taccaacgcc acgtaatgca acacaaatag ctccccagta aacaaatagt
     2761 acgaaaaata aaatatacca tttattactg gcaattgatt ctgccacccc catatcaaca
     2821 tcaataaatg caaccgagac tgcaccaaat gtcagtactg ttggaaagta ggggatgact
     2881 tgtagccagg tcataagcat tcctacaaaa ccccagcgag ccccaaatgc ttctcctatc
     2941 catcgaaatg cccccccttt ttgtggatat gttgtagcta gctcagccgc aacaaaggca
     3001 acaggtacta aaaacataat tgcagcaaag gtatagtaaa aaatggcacc taagccgtat
     3061 tctgcttcag aaggtataaa tcgtagactt actacagcag ttatattcat gatagcgagt
     3121 gtccccactg tcattttctt acttttg
//
LOCUS       DAZPDE010000272         3134 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPDE010000272 DAZPDE010000000
VERSION     DAZPDE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.278"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            15..1055
                     /gene="gatD"
                     /locus_tag="ACHU0J_003944"
     CDS             15..1055
                     /gene="gatD"
                     /locus_tag="ACHU0J_003944"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1218392.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
     gene            1161..1934
                     /locus_tag="ACHU0J_003945"
     CDS             1161..1934
                     /locus_tag="ACHU0J_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1218393.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHU0J_003946"
     CDS             complement(2016..2915)
                     /gene="yegS"
                     /locus_tag="ACHU0J_003946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1218394.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT          828 a          698 c          795 g          813 t
ORIGIN      
        1 aatcaggagt ttttatgaaa tcagtagtga atgatactga tggtatcgtg cgcgttgcaa
       61 aaagcgtcat tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg
      121 gcttatgtgg ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa
      181 cgttaggcca tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac
      241 accctggcga tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt
      301 tgaaagggtt ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg
      361 gatttgctga atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc
      421 ctattgagga tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag
      481 cgcaaggttg tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg
      541 ctattcagtg cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag
      601 aaaaactggc actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga
      661 gcgcgccgca aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga
      721 cggctggcgt accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac
      781 tggcgctggt gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa
      841 tattgcgtaa agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg
      901 ggcaggagtg ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat
      961 taatcgctca ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta
     1021 atgccatgca gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc
     1081 ccggtattgt gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa
     1141 ttgaatatgg gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag
     1201 tgaatgaaca gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag
     1261 cgacaatccg tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg
     1321 gcggtgcggc gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta
     1381 aagagcgatt tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa
     1441 tgatccatga agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg
     1501 aaggattaat gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg
     1561 ccctttccga aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa
     1621 cgcgctcgat gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa
     1681 tgtttgtcgg tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt
     1741 attcgattag cggggcgatg gtcaccgccg caaataaagt gattgccgtt ctcgattcat
     1801 cgaaatttaa tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattattg
     1861 ttaccgatga tgctgtgtca gaggtggata aattggcatt gcagaagaca cgggtaaaat
     1921 taattacggt gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt
     1981 acggattctc cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt
     2041 ggtaatcgac aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac
     2101 ggttcgccat caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa
     2161 gcgccttcga taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga
     2221 agaatttcat cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgagttc
     2281 gggcacaatt gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg
     2341 tcaccttgcc agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc
     2401 atgcgcatta agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc
     2461 ggcgtttctg tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt
     2521 tgtttgttga cctgcgccat atctatcgca atggcattac cggcaattgc cagtttcagc
     2581 gccttatcca gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat
     2641 ggcaaaattc ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact
     2701 tcattaatgg tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc
     2761 tcctctacaa atcgtgcggc atcacctttc tcccaggtga cccgcacatg gatcgtcatt
     2821 ccttcctcac gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttacca
     2881 ttaagaatca gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt
     2941 ctgtagatag tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg
     3001 ataaaaagtc agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg
     3061 acagcgaata ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc
     3121 aactaaacag tggc
//
LOCUS       DAZPDE010000273         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPDE010000273 DAZPDE010000000
VERSION     DAZPDE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.279"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHU0J_003947"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHU0J_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1218395.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACHU0J_003948"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACHU0J_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHU0J_003949"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHU0J_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR1218396.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHU0J_003950"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHU0J_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR1218397.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACHU0J_003951"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACHU0J_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAZPDE010000274         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPDE010000274 DAZPDE010000000
VERSION     DAZPDE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.280"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHU0J_003952"
     CDS             <1..498
                     /locus_tag="ACHU0J_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1218398.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHU0J_003953"
     CDS             578..1273
                     /locus_tag="ACHU0J_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1218399.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHU0J_003954"
     CDS             1302..>3069
                     /locus_tag="ACHU0J_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1218400.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZPDE010000275         3020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPDE010000275 DAZPDE010000000
VERSION     DAZPDE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.281"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(60..179)
                     /locus_tag="ACHU0J_003955"
                     /pseudo
     CDS             complement(60..179)
                     /locus_tag="ACHU0J_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            423..795
                     /locus_tag="ACHU0J_003956"
                     /pseudo
     CDS             423..795
                     /locus_tag="ACHU0J_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1357..2304)
                     /gene="icsP"
                     /locus_tag="ACHU0J_003957"
     CDS             complement(1357..2304)
                     /gene="icsP"
                     /locus_tag="ACHU0J_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR1218401.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          881 a          657 c          594 g          888 t
ORIGIN      
        1 ttaattgatc ctacctacat aatatagaca cagtacctaa agtaccgtgg agatcatccg
       61 gggaagcccc gggtctgata ccagtgcagg tagaccgcta tcttgttccg gtaatgttct
      121 gccgttcttt acggcatcac ctctagcaga aacatcgcgt aactgcgcca agtatgatga
      181 acttctctta ccgctgcgat ctggttcaac aatatgcaga tggtacacaa cctctttaac
      241 cagcccatcc ggattattca gagccgcttc cgcaatccgg aataaaagtg ctgttttacc
      301 gtgaaccctc tgaaaatcag cgagcagtgt tttaaccagc cgacgttcag agcgtactga
      361 cagacggtgg acaatttgaa taaacagttc aatcagaccg tagatgctct cccgttggcg
      421 acatgctggg agcggtggaa caccgcttcg gcaacgagct tccggcgtct ccagtgaagt
      481 gactgacgga taatggttca tgctaccgag ctaatgaaac acgccagttc acccggatgt
      541 tgggactttg ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa cagaatagca
      601 aagagcttcg tgaaaacaat aaagtgtgac tacatcagta tcatgcccaa acctgacggg
      661 ttaacggcag caaagaacct tgcagaggca ttcgagcatt ataacgaatg gcatccgcat
      721 agtgcgctgg attatcgctc gccacgggaa tatctgcggc agcgggccaa tgataacagg
      781 tgtctggaaa tataggggca aagccaccca cgaacgatcc agcatataat caagaaatta
      841 gatttcattt aacataagat agattatgta caccaagagg cgtttcctta atttctgaat
      901 ttacaggctc ttttgctctc tttgctatgc tgtggatttg gaaagataga gtgctggctg
      961 attatttagg tattttcatc agcatgtccc tgatagcact gttccatcag cgctttcagg
     1021 cgataatagt agaccttggg tagcctctgc ccatgtgtaa cttgataccc cgagaacagc
     1081 cacgatgatc taagcggttc ttatttctcc ggtctcaggg tcggtaatgc caacagtaag
     1141 tccacagtaa tcaacgaaga gttttttgaa ccgccccgga aatcctggag actaaacgcc
     1201 ctgagaaaga ggtaaacaga atgactaaaa atacacgttt ttcccccgaa gtccgtcaac
     1261 gggcagttcg tatggttctg gaaagtcagg gcgaatatga ctcacaatgg gcggcaattt
     1321 ttagccatga aaacgtaatc aactcggtga accaattcaa aaaatatact ttatacctgc
     1381 ggaagtgaga aaaccaatat attcaatact tgcactattt ttaatggtgc cagatatatt
     1441 ggtactttgc tcataaagag atgtatcacc ttttttatta gaaatgtaat ttctggagcc
     1501 ttctatataa aattttgcac tgggggtgac atagtatcca atattcgcag caacagagca
     1561 gtaattttgg ttttttattt catctttaaa aattgtctca gtctgatagt gtttatctgt
     1621 atcagatgaa agaacccaac cactatattt cagttctgct ccaaactcaa aattctcatg
     1681 gcggtaatta gcagttaatc caatataagg tattttgaag agctgtttgt aacctattgt
     1741 tctttcacca ctaggatgtg cccctttttt attcctgctc cccccgtttt cactataaat
     1801 ataactccct cccattgcat taaaactgta acgactctct tggtaacctg ctattagtcc
     1861 aagtcgataa tccaaattat ttaataacca acctttaaga ttcagctcga attcgttagc
     1921 atcacggaga tgcgtattgg gatgccagct ctggtcggtc catacctgag gggtattgga
     1981 attgttccag tctttatcaa ccatgcttgc tttctggtta cctaaagtag tccaaccgga
     2041 aactccgaaa gacacttttg gaattagctt ccaatcgatg ccacctctaa caatagtcgc
     2101 attactgtat ttccagtcca gttgactaat ttttctcccc ccttccttgg gatgataaac
     2161 gcgttctttt gttttgccac tcagagatcc tagactaata tccgtactga tgttgtccgg
     2221 tatgaaaagt ggatagttag tggtagcatg tgtagtaaag atcgcaggta cacaaagtgc
     2281 aagtacaaag aattttaatt tcatcgagaa ctctactttt ttggttgaaa tgtccatgat
     2341 cttcttactt tataagataa acgtcaatgc caagcattat tttaattaaa tatttgttta
     2401 tgttaccaag aacatatctt ttattataat atttattgaa cttttgtaaa aataaaaaaa
     2461 tacctcattg tgcgaataaa gtaacggggg ccatagattt ctctacctac cttttggtgt
     2521 tcatacctga ggctgatagt ttcaggattc cgcgacaatt ctactcacct tcttgttctc
     2581 tcgccaatat gaaatcaatc agaaaagcgt tccataagaa caacgataag acaataaatt
     2641 gtcatcacgg ctgcaatagc caacgtgcct ttcagtacca acatgacaac ctcctgtatt
     2701 tgacgtaacg tgtgctgata aatatgtatt tgtatctcaa ctgactcaat acaacactta
     2761 agtatagttt ttcagatttt gtttttcccg tgaaatatta tggaaatggc agaagcaata
     2821 cattgaattc cgattcgagt gaatacggct agcgggcacc tcactttagc actgaagcca
     2881 tcaactttta tttgacttat ttcaatggaa agctttctca ctatcagtac aggtaattcc
     2941 catcctttta aagtcgagag tcagactgcg actaatacag ttaaagtgac caaactcagt
     3001 tatcctctac acattttatc
//
LOCUS       DAZPDE010000276         3016 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPDE010000276 DAZPDE010000000
VERSION     DAZPDE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.282"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHU0J_003958"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHU0J_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1218402.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHU0J_003959"
     CDS             348..641
                     /locus_tag="ACHU0J_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1218403.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHU0J_003960"
     CDS             713..1372
                     /locus_tag="ACHU0J_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1218404.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHU0J_003961"
     CDS             1464..1910
                     /locus_tag="ACHU0J_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1218405.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3016)
                     /gene="hlyE"
                     /locus_tag="ACHU0J_003962"
                     /pseudo
     CDS             complement(2117..3016)
                     /gene="hlyE"
                     /locus_tag="ACHU0J_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          787 a          651 c          721 g          857 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtca
//
LOCUS       DAZPDE010000277         2952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPDE010000277 DAZPDE010000000
VERSION     DAZPDE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.283"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHU0J_003963"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHU0J_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1218406.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHU0J_003964"
     tRNA            complement(621..695)
                     /locus_tag="ACHU0J_003964"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHU0J_003965"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHU0J_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHU0J_003966"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHU0J_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1218407.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          729 a          710 c          700 g          813 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta ac
//
LOCUS       DAZPDE010000278         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPDE010000278 DAZPDE010000000
VERSION     DAZPDE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.284"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHU0J_003967"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHU0J_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1218408.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHU0J_003968"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHU0J_003968"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1218409.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHU0J_003969"
     CDS             2603..>2951
                     /locus_tag="ACHU0J_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1218410.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZPDE010000279         2942 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPDE010000279 DAZPDE010000000
VERSION     DAZPDE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.285"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHU0J_003970"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHU0J_003970"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR1218411.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHU0J_003971"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHU0J_003971"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR1218412.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHU0J_003972"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHU0J_003972"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR1218413.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHU0J_003973"
     CDS             complement(1895..2809)
                     /locus_tag="ACHU0J_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR1218414.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          773 a          684 c          784 g          701 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattaccta gaaccacggc agcctgacgg ctgcaaattg aaaactgccc ctgttcgtcc
     2941 tg
//
LOCUS       DAZPDE010000280         2933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPDE010000280 DAZPDE010000000
VERSION     DAZPDE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.286"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHU0J_003974"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHU0J_003974"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1218415.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHU0J_003975"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHU0J_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1218416.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHU0J_003976"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHU0J_003976"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR1218417.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2933
                     /locus_tag="ACHU0J_003977"
     CDS             2756..>2933
                     /locus_tag="ACHU0J_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218418.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          801 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt ggc
//
LOCUS       DAZPDE010000281         2916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPDE010000281 DAZPDE010000000
VERSION     DAZPDE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.287"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1545
                     /locus_tag="ACHU0J_003978"
     CDS             <1..1545
                     /locus_tag="ACHU0J_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR1218419.1"
                     /translation="FTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREV
                     VDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARF
                     GVYSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDN
                     FTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIG
                     VVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGV
                     KRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWW
                     KGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLT
                     WITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTV
                     ENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKK
                     ALSEAK"
     gene            1597..2754
                     /locus_tag="ACHU0J_003979"
     CDS             1597..2754
                     /locus_tag="ACHU0J_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1218420.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2720..2842)
                     /locus_tag="ACHU0J_003980"
     CDS             complement(2720..2842)
                     /locus_tag="ACHU0J_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218421.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          887 a          633 c          586 g          810 t
ORIGIN      
        1 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact aaccatggat
       61 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
      121 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa
      181 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
      241 gtggcacacg gtgtttccgg cccctcccgt gccgcaataa tgaccggtcg agctcccgcc
      301 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
      361 ttgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
      421 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac
      481 ttcaccacat tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg
      541 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa
      601 cgtgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
      661 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac
      721 ctaccaaatg ataatcctgc accggatcaa tatcagaagc aatttaatac cggtagtcaa
      781 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
      841 gaacaactga agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat
      901 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
      961 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     1021 ggtaattatg acaagctgat ttccgcaatg gatttctacc caacagctct tgatgcagcc
     1081 gatatcagca ttccaaaaga ccttaagctg gatggtgttc ccttgctgcc ctggttgcaa
     1141 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     1201 tttgatgagg aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca
     1261 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     1321 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     1381 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     1441 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag
     1501 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     1561 gcggattttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     1621 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     1681 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgaa
     1741 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     1801 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     1861 aatacattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     1921 catgaatttc tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga
     1981 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     2041 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattatcct
     2101 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     2161 ctagaaaccg ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc
     2221 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     2281 tgggttaaaa acgatgtggg tgaaattttc attcgtcagt ttgaaagctt tgtcagccgt
     2341 tttttgggga atgggcatac aagttgtatt ttccaggagt cctgcaagga taatctggtt
     2401 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     2461 ggaaacatta ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     2521 aaacggattt cagcgaaatg tcagcaatgt gcatataaac ctatctgcaa tggcggttgt
     2581 cctaagcatc gtattactaa agtaaacaat gagactgttt cttatttttg cgaaggttat
     2641 aaaatccttt tttcaaccat ggtaccttat atgaacgcca tggttgagtt agctaagaac
     2701 agagtaccgc tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc
     2761 tctcttttaa ctccattctt agcctccttc ttatgacctt aacaaggcac ccgacataaa
     2821 ttcctgtcgg gtgtttcgtc atcatcatct tcccttgcga aaatcctggc ttacgtggta
     2881 tgcgtcaggg cattagcaat acccttgatt atcaat
//
LOCUS       DAZPDE010000282         2894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPDE010000282 DAZPDE010000000
VERSION     DAZPDE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.288"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..308)
                     /locus_tag="ACHU0J_003981"
     CDS             complement(<1..308)
                     /locus_tag="ACHU0J_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1218422.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHU0J_003982"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHU0J_003982"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1218423.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHU0J_003983"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHU0J_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1218424.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZPDE010000283         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPDE010000283 DAZPDE010000000
VERSION     DAZPDE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.289"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHU0J_003984"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHU0J_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1218425.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHU0J_003985"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHU0J_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1218426.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHU0J_003986"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHU0J_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR1218427.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZPDE010000284         2860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPDE010000284 DAZPDE010000000
VERSION     DAZPDE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.290"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(77..1501)
                     /gene="patD"
                     /locus_tag="ACHU0J_003987"
     CDS             complement(77..1501)
                     /gene="patD"
                     /locus_tag="ACHU0J_003987"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1218428.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1523..2317)
                     /locus_tag="ACHU0J_003988"
     CDS             complement(1523..2317)
                     /locus_tag="ACHU0J_003988"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1218429.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2307..2654)
                     /locus_tag="ACHU0J_003989"
                     /pseudo
     CDS             complement(2307..2654)
                     /locus_tag="ACHU0J_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          713 a          846 c          688 g          613 t
ORIGIN      
        1 ttctaatact taaccgccat taaactcaga acctaaaacg atagtggccg tcctataaca
       61 gccactaaca tctaacttaa tgtttaacca tgacgtggcg gacgacggtg taatcctcca
      121 gcccataaag tgacatatcc ttgccgtaac cagaaagttt ctgcccaccg tgcggcattt
      181 cacttaccag catgaaatgg gtattgaccc aggtacaacc atattgcagc cgtgcgctga
      241 cgcgatgcgc cctgcccaca tctttcgtcc ataccgaaga tgcaagtccg tactggctgt
      301 cattcgccca gttcaccacc tgttcttcgt tgtcgaaggg cgtaacactc actactggac
      361 caaatacctc tttttgcacg atggcatcgt cctgtaatgc gccagccagc agcgtcggcg
      421 catagtaata gccattaccc ttgcgctttt caccgccagt gatcactttg atgtgccctg
      481 tcgctttcgc ctcttctact gccttgctga cgcgttcgag atgcgccagc gagcttaaag
      541 gtccaagctc cgtagactcg tcatccggcg caccagattt taacgttgcc accgcagcac
      601 ccagtttttc caccagcgta tcgtaaatgc ctttttgcgc gtagatccga caagccgcag
      661 tacaatcctg tccagcattg taatagccaa atgtacgtac accttcgacc actgcttcaa
      721 tatccgcatc atcaaaaaca atcactggcg ctttgccacc aagttccata tgagtacgct
      781 taatggacga cgcggtatgg ctgatgatgt gctcgccggt ggcgatagag cccgtcagcg
      841 acaccatccg cactttggga tgaccggtca gcggatcacc caccgttttg cctctgccaa
      901 acagtatgtt aatcacgcct gccgggaaga tatctttcgc cagctctgcc aacttcaacg
      961 cggtcagcgg ggtaatttct gatggtttaa gcactacgca gttccctgcc gccagcgccg
     1021 gagcaagttt ccacgcggcc atcatcagcg gataattcca cggtgcgata gaagccacga
     1081 cccccaacgg atcgcgacgg atcatcgaag tatgaccttc aagatattca cctgccgcca
     1141 gaccattcag acagcgcgcc gcacccgcga aaaagcgaaa aacatcgaca atcgccggga
     1201 tttcatcatt gaacgcacta tgcagcggtt tgccacaatt acgggattcc agttcggcaa
     1261 aaacctgacc attttcttcg ataacatccg ccagtttcag caggcattcc gcacgcgctt
     1321 tcggtgtggt ttgcccccat tcagcaaatg ctgcatctgc cgcacgcaca gcagcatcga
     1381 cctgctctgc ggatacctcg gcaatctcca gtaaaacgtc ccccgttgcc ggattataga
     1441 caggctgttt ttccccttcg ccgctaaccc gttctccgtt aatcagtaac ttatgttgca
     1501 tagcttattc ctgtatcagt ggttattgac cattgtcgcc ttcgcgggtt agccaccagg
     1561 cccccaggat cggcaaggtt gttaccaaca taaccagcag tgccaccacg ttggttaccg
     1621 gtacatcacg cggtcgccca agctgattga gcaaccacaa cggtaacgtt cgttcatgac
     1681 ctgccgtaaa ggtcgtaacg atgatttcat cgaacgacaa ggcaaacgcc agcattcctc
     1741 ctgccagtaa cgccgaactg agattcggca acactacgta gcggaaggtt tgccagccat
     1801 tggccccaag atccattgac gcctcaacca gactccacga ggtgcggcga aaacgggcga
     1861 tgacattgtt aaacaccacc actacgcaaa aagtcgcatg accgaccacg atggtgaaaa
     1921 atcccggctc cagattgatg gttttaaagg cggttaatag cgccagacca gtgacaatgc
     1981 ccggcagcgc aatgggcagc agcaataaca gcgaaatggc gtttttgccg aaaaagtctc
     2041 gtctccacag cgcggcagct gccagcgtcc ctaacaccag cgcaattaat gtcgccagcg
     2101 ccgccacttt aagtgacagc gtcacggcat caagaatatc actacgctgt gctgccacgc
     2161 taaaccagcg cagcgtcagg ccctgcggtg gaaaactaaa cgccgcatct tcagtgttaa
     2221 acgcataggc ggcgattatc aggatgggaa aatgtaggaa aacaacgccg ccccaggccg
     2281 ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga acgctcccag acgtttcacg
     2341 aacgccaggt acagtgcgat gagaataatc ggcaccaggg tgaatgccgc cgccatcggc
     2401 atattgccaa tcgccccctg ctgggaataa accatattgc cgataaaata tcctggagga
     2461 ccaaccagct gcgggacgat aaaatcgccc agtgtgagtg agaaggtaaa gatagagcca
     2521 gcggcaatac ccgggattgc cagcggcagc accacatagc gaaaggtttg tcgtggacgt
     2581 gcgccgagat cagccgacgc ctgcaacaat gacggcggca aacgctcaag cgccgcctga
     2641 acgggcagga tcatcgagta ggcagcctga cggctgcggc ttgtcatggt ctgcaattac
     2701 cgttataaaa acaggcaatg tcattgtctt tcaggtggtt atatggcccg ttcagctaaa
     2761 ccccgtaaac gtaaacccgc ctctcaaagt tcctgagtcg tgacccgttc gaatgcgtgc
     2821 tgtgtggcgg ccggatggta taccgccggg ccattgcggg
//
LOCUS       DAZPDE010000285         2842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPDE010000285 DAZPDE010000000
VERSION     DAZPDE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.291"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            110..2389
                     /gene="ydeP"
                     /locus_tag="ACHU0J_003990"
     CDS             110..2389
                     /gene="ydeP"
                     /locus_tag="ACHU0J_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1218430.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2637..2840
                     /gene="safA"
                     /locus_tag="ACHU0J_003991"
     CDS             2637..2840
                     /gene="safA"
                     /locus_tag="ACHU0J_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1218431.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
BASE COUNT          758 a          656 c          691 g          737 t
ORIGIN      
        1 gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
       61 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
      121 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
      181 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
      241 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga
      301 catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc
      361 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
      421 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
      481 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
      541 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
      601 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
      661 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
      721 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
      781 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
      841 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
      901 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
      961 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1021 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     1081 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     1141 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     1201 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     1261 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     1321 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     1381 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     1441 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     1501 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     1561 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     1621 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     1681 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     1741 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     1801 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     1861 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     1921 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     1981 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     2041 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     2101 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     2161 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     2221 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     2281 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     2341 tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     2401 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     2461 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     2521 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     2581 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc
     2641 atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg
     2701 taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc
     2761 agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     2821 gtttgcaaga ctggaaataa ac
//
LOCUS       DAZPDE010000286         2835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPDE010000286 DAZPDE010000000
VERSION     DAZPDE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.292"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHU0J_003992"
     CDS             complement(<1..593)
                     /locus_tag="ACHU0J_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1218432.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHU0J_003993"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHU0J_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR1218433.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHU0J_003994"
     CDS             complement(1422..1961)
                     /locus_tag="ACHU0J_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1218434.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHU0J_003995"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHU0J_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR1218435.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          735 a          705 c          622 g          773 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagat
//
LOCUS       DAZPDE010000287         2825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPDE010000287 DAZPDE010000000
VERSION     DAZPDE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.293"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            301..1467
                     /gene="nhaA"
                     /locus_tag="ACHU0J_003996"
     CDS             301..1467
                     /gene="nhaA"
                     /locus_tag="ACHU0J_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1218436.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1533..2432
                     /gene="nhaR"
                     /locus_tag="ACHU0J_003997"
     CDS             1533..2432
                     /gene="nhaR"
                     /locus_tag="ACHU0J_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1218437.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2470..>2825)
                     /locus_tag="ACHU0J_003998"
     CDS             complement(2470..>2825)
                     /locus_tag="ACHU0J_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1218438.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          665 a          625 c          711 g          824 t
ORIGIN      
        1 acgacaagct ggattatttt tgaaatattg gcctaacaag catcgccgac tgacaacaaa
       61 ttaattatta cttttcctaa ttaatccctc aggaatcctc accttaagct atgattatct
      121 gggcttaggg tcactcgtga gcgcttacag ccgtcaaaaa cgcatctcac cgctgatggc
      181 gcaaattctt caatggctcg taaaaaacga attattccta cactataatc tgattttaac
      241 gatgattcgt gcggggtaaa atagtaaaaa cgatctattc acctgaaaga gaaataaaaa
      301 gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt
      361 gccgctatcc tggcgatgat tatggccaac agcggcgcaa ccagtggatg gtatcacgac
      421 tttctggaga cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg
      481 ttatggataa atgatgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa
      541 cgtgaactga tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc
      601 gctattggtg ggatgattgt gccggcatta ctctatctgg cttttaacta tgccgatccg
      661 attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcttttgc acttggtgta
      721 ctggcgctgt tgggaagtcg tgttccgtta gtgctgaaga tctttttgat ggctctggct
      781 attatcgacg atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg
      841 atggcctccc ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt
      901 ggtgtacgcc gcacgggcgt ctatattctt gttggcgtgg tgttgtggac tgcggtgttg
      961 aaatcggggg ttcacgcaac tctggcgggg gtaattgtcg gcttctttat tcctttgaaa
     1021 gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttgcaccc gtgggtggcg
     1081 tatctgattt tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggcgtcacg
     1141 ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa
     1201 ccgctgggga ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct
     1261 gagggaacga cttatcagca aattatggtg gtggggatcc tgtgcggtat cggttttact
     1321 atgtctatct ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg
     1381 gcgaaactcg gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta
     1441 cgcgttcgtt tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc
     1501 ccttttcatt gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt
     1561 attacttctg gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt
     1621 taactccaca aaccattacc ggacagattc gagcgctgga agagcgcctg caaggcaaat
     1681 tatttaaacg caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct
     1741 atgccgataa aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag
     1801 aatccaattt attgtttgac gttggcgtgg ctgatgcact ttccaaacgc ctggtcagta
     1861 gcgtacttaa cgccgcagtg gtagaaggcg agcccattca tcttcgctgc ttcgaatcca
     1921 cccacgaaat gctgctggag caattaagtc agcataaact ggatatgatc atttctgact
     1981 gtccgataga ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg
     2041 tgagtttctg gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac
     2101 ggcgactttt gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta
     2161 actcccaggg attaaacgta gaaatcctcg gcgagtttga tgatgccgct ttgatgaaag
     2221 cttttggtgc gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt
     2281 atgccgataa aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg
     2341 ctatttttgc tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt
     2401 attctgcgct ttttagtcca gcggtgcgtt aatcggcagc tcccccaaag ttaaggtggg
     2461 ggagatagat tagttgtaca ttaccacgat tttgactcgg ctcattattt gcccgcttga
     2521 gacattgttt ccatatgtac gcgggcgaat aaatagagga atctgattac ttccttcatg
     2581 gggatgctga aaagagtagt aattgctttt accatactgg atttcattcg taccatcatt
     2641 ggagagttga aagcctattt gacccgcacc gtcctcactg ttttctaact cattttttaa
     2701 tatgctggtt cctgactcct gtatcgtcac tggttcaaag taatattcaa cgtcactggg
     2761 ttcaaaaact aattcacctt gctcgccctg gatgcagtca aatcttatat gatgaaactg
     2821 ataat
//
LOCUS       DAZPDE010000288         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPDE010000288 DAZPDE010000000
VERSION     DAZPDE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.294"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHU0J_003999"
     tRNA            6..81
                     /locus_tag="ACHU0J_003999"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHU0J_004000"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHU0J_004000"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHU0J_004001"
     CDS             complement(299..1057)
                     /locus_tag="ACHU0J_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1218439.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHU0J_004002"
     CDS             complement(1065..2168)
                     /locus_tag="ACHU0J_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1218440.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHU0J_004003"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHU0J_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR1218441.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZPDE010000289         2800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPDE010000289 DAZPDE010000000
VERSION     DAZPDE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.295"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            95..2248
                     /locus_tag="ACHU0J_004004"
     CDS             95..2248
                     /locus_tag="ACHU0J_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1218442.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>2800
                     /locus_tag="ACHU0J_004005"
     CDS             2301..>2800
                     /locus_tag="ACHU0J_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218443.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVP"
BASE COUNT         1024 a          507 c          513 g          756 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga
//
LOCUS       DAZPDE010000290         2792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPDE010000290 DAZPDE010000000
VERSION     DAZPDE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.296"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHU0J_004006"
     CDS             <1..203
                     /locus_tag="ACHU0J_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218444.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHU0J_004007"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHU0J_004007"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1218445.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..>2792
                     /locus_tag="ACHU0J_004008"
     CDS             2102..>2792
                     /locus_tag="ACHU0J_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1218446.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQK"
BASE COUNT          772 a          618 c          626 g          776 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca caattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa ag
//
LOCUS       DAZPDE010000291         2777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPDE010000291 DAZPDE010000000
VERSION     DAZPDE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.297"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            430..573
                     /locus_tag="ACHU0J_004009"
     CDS             430..573
                     /locus_tag="ACHU0J_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1218447.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            586..795
                     /gene="cspE"
                     /locus_tag="ACHU0J_004010"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             586..795
                     /gene="cspE"
                     /locus_tag="ACHU0J_004010"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1218448.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            961..1770
                     /gene="rlmA"
                     /locus_tag="ACHU0J_004011"
     CDS             961..1770
                     /gene="rlmA"
                     /locus_tag="ACHU0J_004011"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1218449.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1767..2333)
                     /gene="mntP"
                     /locus_tag="ACHU0J_004012"
     CDS             complement(1767..2333)
                     /gene="mntP"
                     /locus_tag="ACHU0J_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1218450.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          758 a          632 c          647 g          740 t
ORIGIN      
        1 gatttttttt tgctcactca tcaatttttc ttattttaaa tttacaatcc ctttggggat
       61 tgacttctct ttagggtaat tgatagccgt taactgactg ttttatgaga aaaagtgata
      121 taacttttta ttcattgcat agcagaaaat gtgatattgc acgcactatg taataacttc
      181 tccctctggc ctggaacaac tgaacttatt gaactatgtt agaaactacg ccagtttaag
      241 tatctgcctg aactggcaag gttaagcaca atgatatatc ggcgcgtatt ccgttgcata
      301 agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg tctgatcgag atatgtttaa
      361 aaatggcttg ccataattaa cgttgtatgt gataaaagat ttcgggttaa acgaggtaca
      421 gttctgttta tgtgtggcat tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa
      481 taccgcttct ctgccgagcc ttatattggt gcctcatgca gtaatgtgtc agttttatct
      541 atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt ttcaaatggc aaagattaaa
      601 ggtcaggtta agtggttcaa cgagtctaaa ggttttggct tcattactcc ggctgatggc
      661 agcaaagatg tgttcgtaca cttctccgct atccagggta atggcttcaa aactctggct
      721 gaaggtcaga acgttgagtt cgaaattcag gacggccaga aaggtccggc agctgttaac
      781 gtaacagcta tctgatcgaa tccactgatc tgaagtgtga atacgcttca atctcgctat
      841 aaagcctcgt cgaatgcgag gctttttact atgctttatc ttcgctcctg gcgttcggat
      901 atttgcccgc cgcgtgattc gcgttacact tgcggccttt agtatcctgc cggagttgtc
      961 atgtcgtttt cctgtccact ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc
     1021 tgtccccaac gacatcagtt tgatatggcg aaagaagggt atgtcaatct gctgcccgtt
     1081 cagcataaac ggtctcgtga tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca
     1141 ttcttagatg ccggacatta tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa
     1201 cggcttgatg aaaaggccac ggcggtgctg gatattggct gtggtgaagg gtattacaca
     1261 cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg gcctggatgt ctcgaaggta
     1321 gcgataaaag cggcggcgaa acgctatccg caggtcactc tttgtgtcgc ttccagccac
     1381 cgtttgccgt tttccgatac cagtatggac gccataatac gtatttacgc gccgtgtaaa
     1441 gcagaagaat tagcacgagt agtgaagccc ggcggctggg tcattactgc cacgccggga
     1501 ccgcgacatt tgatggagct gaaggggctg atttacaatg aagtacatct tcatgcacct
     1561 catgcagaac aactggaagg ttttacatta cagcagagtg cggagttgtg ttatccgatg
     1621 cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg
     1681 aagccagaag tctggcaaac actggcagca aaagaagtgt tcgactgcca gacggacttt
     1741 aatattcacc tctggcagcg ttcttattaa ccgtggaagt gcgtccagag gatctggacg
     1801 ccgatgccga tcagcaccag cccgccgaga atttccgctt ttttcccaat aattgagccg
     1861 ataaagcgac caaccatcat ccctaatgtc gacataatca aggttgcaca accaatggcc
     1921 aatgcggtcg cgataatgtt gacctgaagg aaagcaagac caacacccac agccatggca
     1981 tccaggctgg tggcaatcgc ggtggttacc agtagccaga aaccgtgccg acggcgcggc
     2041 tcttcatctt catcatctgc gccacgaaaa ccctcaataa tcattcgccc gccgaggaat
     2101 atcagcagca caaacgcaat ccagtgattc cattcaagga caaaccggct ggctaacatg
     2161 cccattcccc agccgatcag cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt
     2221 cgcaatgctt cagaaaattt cggtttatgg aggggggcac ctttaccgat tgatgcagca
     2281 aatgcatcca tcgacatacc aaacgcaaga agaacagtag cagtgatatt catgacaacg
     2341 tcctgaccgg ggtatccata aaacacatca tcgcccccag caaacatgca tacatgaaca
     2401 acaagccgcg atgggttttc gcgttcatgc aggcatagcg attgatgtgc atatggtctc
     2461 gcctgactga aagtcttcag tccgtacgtg ccacgttttg caacgagtat gttgacacgt
     2521 acatttccgg aatctggaaa tcggctactc cccaaatgag ggcgcaacct taacatattt
     2581 tgagaatata aaacaactgt tggaatatat tatttcattg ctttattgat aacgattttc
     2641 atttatgttt atatatcaat gaaacgttaa aataaataac aaataaggga agagataata
     2701 tagcctaagc tatgtctgat tattaaaaat acactaaaca tagctttggc taaattcata
     2761 agttattgag ttgatac
//
LOCUS       DAZPDE010000292         2715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPDE010000292 DAZPDE010000000
VERSION     DAZPDE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.298"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            6..224
                     /locus_tag="ACHU0J_004013"
     CDS             6..224
                     /locus_tag="ACHU0J_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1218451.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="ACHU0J_004014"
     CDS             226..591
                     /locus_tag="ACHU0J_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1218452.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="ACHU0J_004015"
     CDS             588..1253
                     /locus_tag="ACHU0J_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218453.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="ACHU0J_004016"
     CDS             1253..1618
                     /locus_tag="ACHU0J_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1218454.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="ACHU0J_004017"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="ACHU0J_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1218455.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2504..>2715
                     /locus_tag="ACHU0J_004018"
     CDS             2504..>2715
                     /locus_tag="ACHU0J_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218456.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          772 a          557 c          696 g          690 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgtta
//
LOCUS       DAZPDE010000293         2712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPDE010000293 DAZPDE010000000
VERSION     DAZPDE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.299"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHU0J_004019"
     CDS             50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHU0J_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1218457.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2712
                     /locus_tag="ACHU0J_004020"
     CDS             2583..>2712
                     /locus_tag="ACHU0J_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1218458.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          678 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc gg
//
LOCUS       DAZPDE010000294         2648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPDE010000294 DAZPDE010000000
VERSION     DAZPDE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.300"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(269..1048)
                     /gene="ipaJ"
                     /locus_tag="ACHU0J_004021"
     CDS             complement(269..1048)
                     /gene="ipaJ"
                     /locus_tag="ACHU0J_004021"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR1218459.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1245..2054)
                     /locus_tag="ACHU0J_004022"
                     /pseudo
     CDS             complement(1245..2054)
                     /locus_tag="ACHU0J_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2401..>2648
                     /locus_tag="ACHU0J_004023"
     CDS             2401..>2648
                     /locus_tag="ACHU0J_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218460.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          784 a          670 c          433 g          761 t
ORIGIN      
        1 aattacaacg agtgactaaa ataaaaatat catccaagtc aatcactgtt tgctctgcaa
       61 aatatgtcag ccttctcaaa cttaccagca cttaaatatt taagcacaca atcattcctt
      121 gagggagttc acttatttca agagaaacat ttcgtcttcc cacacaacta atccgcccaa
      181 tatagctaaa tttgtactca gtaatatcat ttgttatgcc cccccacccc aaaatattca
      241 agctccttca cagataaaaa acgacaattt acaaagcctc attagttata actatggaaa
      301 tacctatttt agtatatcct ataaagttag aattacttct tcttgcacct aattccatag
      361 ttgaaaaaca agaataattt tctcctacag ctggatccat atagcttcca tcaggtctac
      421 acaacaccca gtggaggccg acaggaactc caataaagga tacagcaaca gcttccaaaa
      481 ctctttggtt agaagataac acatctcgtt gatgtactat attacatccc atgccaatta
      541 ataagtccct tgcatcggga taaataaaac taattacttg cgaaaaaatg ttactttctt
      601 caacgacata agcattcaat ccaagaagcc tcgtagccat gactatctta tcaggcatag
      661 aataaccggc atccgcgata ttatctttgt gtattctcgg attataatta ccactagtta
      721 ttaagtataa atcccgctca caacgattat ccaaatcaag agaactcttc cttgtcattt
      781 cggacataga ccctttgtat tgtggtattt tatcaacccc taattccttt gcagcacaga
      841 gtaaactggc agctccacaa gaattgtcat acatctgctt cctcgccaaa aaacaacctt
      901 gttcgtttac tctattctca tttaccctca catcagtctg atgagatatc atatattctc
      961 ttggtatagc cccctgcaat aaaaccccat ataacataac acctgtatga atacatcccc
     1021 gtttgcaagg cttccgttgt tccgacatcg acgagtaatt gcagcacagt attgcatcca
     1081 gcaatgcgaa ctactatgag aattagctga ccgggtcctt attccccgaa cgaactttaa
     1141 atccaaaacc tcatactctt ttccgtcgtc atcacgtccg tcaccataac aacctttcag
     1201 catccaccct tttattaaga gttaatgtcc atcagcatca gcaattaata ccgcctgata
     1261 ctgacatatt tttgtcgtat ttacgtccat aatcggttat tgtgatacag gtcacaccag
     1321 cttatttgct attgttacaa taaccacctt ggttgagagc tgttttttca actttgttat
     1381 caactccccc aactccttgg tcagtaaggc tgccgaccga gcacagtgaa tagatagtat
     1441 ccgcaggtac caccatgctt acttaatact cagaaggcga agtgctctcc aattaactct
     1501 gccgcactat gcttataagc tgcttttcaa tgccgtctat caacatgtcc aattcattca
     1561 atctgttgta ctggtaatcc aacgcagtaa tgatatatgc aggtattctt tcatccactc
     1621 cagtaacgtt tcatgcaaag cattaattta cgcgctacgg aacttcacca gttgactgag
     1681 caaacgatgt gggaccagta ctgcctgcag ttcttctatt gttacagcga tttctttacc
     1741 aaacagctga accaccatca agataaagat agcctgaaca ttcatcgcac cattttgtta
     1801 ccaatggcaa aagatttaac gaaccgtgcc tgcatcaggc ggacttttta tccctgcttt
     1861 gttagctccc taaccttgta cagagagcct ccataagctc ccatatcaag cagacatggt
     1921 tcgcagttac tgacatacac cagaaaactc tgacagcaga ctcacttatc ctccacttca
     1981 ctcatatcct tattggtgag atgaacctga atgacatgtt ttgcgacata aacgccaacc
     2041 agtgtatatt tcacctgtag agattctaca atctgtagcg ttatgcatct aatgttaggc
     2101 tctatgatgt cagaaatctg cgagactcta caccttctgc atttaccttt atttatcacc
     2161 atgctcctca gttaacggag aacgtgttca ttgcattttg tcacttacat ttcgcctttg
     2221 gtatcggggg gatataggtt ccaacgttcc gcatgagaac ctgaaccggc ttcacgccac
     2281 cttgatacca gatgccgcac aggtagtcaa caagtgtcac gaacggtgca atagtgatcc
     2341 acacccaacg cctgaaatca gatccagggg gtaatctgct ctcctgattc aggagagctt
     2401 atggtcactt ttgagacagt tatggaaatt aaaatcctgc acaagcaggg aatgagtagc
     2461 cggacgattg ccagagaact ggggctctcc cgcaataccg ttaaacgtta tttgcaggca
     2521 aaatctgagc cgccaaaata tacgccgcga cctgctgttg cttcactcct ggatgaatac
     2581 cgggattata ttcgtcaacg catcgccgat gctcatcctt acaaaatccc ggcaacggta
     2641 atcgctcg
//
LOCUS       DAZPDE010000295         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPDE010000295 DAZPDE010000000
VERSION     DAZPDE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.301"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..441
                     /gene="ipgB2"
                     /locus_tag="ACHU0J_004024"
     CDS             <1..441
                     /gene="ipgB2"
                     /locus_tag="ACHU0J_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR1218461.1"
                     /translation="INTSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEH
                     SRKIGDNLRKQIFKQVEKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSV
                     KNDTTSNVANLISDQFFEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(703..825)
                     /locus_tag="ACHU0J_004025"
                     /pseudo
     CDS             complement(703..825)
                     /locus_tag="ACHU0J_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            968..1522
                     /locus_tag="ACHU0J_004026"
                     /pseudo
     CDS             968..1522
                     /locus_tag="ACHU0J_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1861..2222
                     /locus_tag="ACHU0J_004027"
                     /pseudo
     CDS             1861..2222
                     /locus_tag="ACHU0J_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2309..>2556
                     /locus_tag="ACHU0J_004028"
     CDS             2309..>2556
                     /locus_tag="ACHU0J_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218462.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          713 a          573 c          595 g          675 t
ORIGIN      
        1 ataaatacta gtattttgag ctctgtttca gaacaaatcg gtgaaaatat tacagattgg
       61 aaaaatgatg aaaaaaaagt ctacgtatcc cgggtggtaa accaatgtat tgataagttt
      121 tgtgcagaac acagccgaaa aatcggcgat aatctccgta aacagatatt taaacaggtt
      181 gagaaagatt atcgcatttc tctagacatc aatgctgcac aaagttccat aaatcatctt
      241 gtatcaggaa gcagttattt caaaaagaaa atggacgaac tttgtgaagg gatgaataga
      301 agcgttaaaa atgatacaac aagcaatgtt gcaaatctta tttccgatca gttttttgag
      361 aaaaatgtgc aatatattga tctaaagaaa ttacgtggga atatgagtga ctatattaca
      421 aatttagaat cgcctttctg atgacagaac atcccgagta aagaaactaa gaaaagtaaa
      481 tacccaggaa ttgcctgagc ggtgctatat ttctcctcgc aagtaagtcc taatatattg
      541 aattgttgta tagccagttt ttgtattttc ctagctcatg gatgtgggat ccctctcccc
      601 tttctctgct caaaggtaag cggctcgtca gaatcatatt gatatttact gagaagtcag
      661 atcaactttc caaggcaaca gatcgcgtac ccggtttgcc ggtcagtcct ggatatgttc
      721 aataacgtaa cgcagccatt tttctggctc cacattgttc agacggcatg tgccgatcag
      781 cgagaacaac accgctgcat gttcgtcacc gctgtcggaa cctgcgagtc gagtcagccg
      841 gagagagttt cactctccgg ctgcttacgg aaccgtacgt gacactctcg cgtcatacgg
      901 ctcttgatca ctcattcgat gcacgtagta aataccagtg agcaaattga tcttacccag
      961 caatacggtc agttaccgcg cacatggcct gcctgtgtca gaaaatctgc tggagcagga
     1021 tttttacgcc agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga
     1081 tgaaggctgg ctgtatctgg ctgtggtcat tgacctgtgg tcacgtgccg ttattggctg
     1141 gtccatgtca ccacgcctga cggcgcaact ggcctgcgat gccctgcaga tggcgctgtg
     1201 gcggcgtaag agaccccgga acgttatcgt tcactcggac cgaggaagtc agtactgttc
     1261 agcagattat caggcgctgc tgaagtggca taatctgcgt ggcagtatga gcgcaaaggg
     1321 ctgctgctac gataatgcct gcgtggaaag cttctttcat tcgctgaaag tggaatgtct
     1381 ccacggagaa cactttatca gccgggaaat aatgagggca acagtgttta attatatcga
     1441 atgtgattac aatcgctggc gacggcacag ttggtgtggc ggcctcagtc cggaacaatt
     1501 tgaaaaccag aacctcgctt aggcctgtgt ccatattacg tgggtaggat cacttacgcc
     1561 ctgtggttga aacgtgagca tattgttttc agatgctgtt gtcttgcttc agagtgatct
     1621 gtcctccgga tttacgcaaa gttatccaca ggcactgtgc atttaaaaaa cttatctttt
     1681 cttcttgatt ttaaaaactc agatcgtcat atcattatga taaacataat aaaaaactct
     1741 aaacatgaga aggtttttaa cgtaaggtga gaggattttt aagtgtatcc atagattaaa
     1801 aatgacaatg cctccaatta gttgaattag gggttataat acccgctttt aggttctttc
     1861 atggtcacag ttaactttca ttgcccccgt tgccagtccg ttcaggttta ccgtcacggg
     1921 cagaacccaa aaggacatga tcgattccgt tgccgtgact gtcaccgggt gtttcagctc
     1981 acttactgtt acgaggcccg taaaccggga gttaaaaacc aaatcactga aatggctttc
     2041 aacggtgccg gagttcgcga tactggccgt acgctgaaaa tcggcatcaa taccgtcata
     2101 cggacattaa aaagctcccg ccccggcgga taacatctgc gccagtcgct cacgctgatg
     2161 ttgcccttat ctgtgaactt gatgggcagt ggagcttcgt tggcagcaag gcgcaacaac
     2221 actgtcacga acggtgcaat agtgatccac acccaacgcc tgaaatcaga tccagggggt
     2281 aatctgctct cctgattcag gagagcttat ggtcactttt gagacagtta tggaaattaa
     2341 aatcctgcac aagcagggaa tgagtagccg ggcgattgcc agagaactgg ggctctcccg
     2401 caataccgtt aaacgttatt tgcaggcaaa atctgagccg ccaaaatata cgccgcgacc
     2461 tgctgttgct tcactcctgg atgaataccg ggattatatt cgtcaacgca tcgccgatgc
     2521 tcatccttac aaaatcccgg caacggtaat cgctcg
//
LOCUS       DAZPDE010000296         2547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPDE010000296 DAZPDE010000000
VERSION     DAZPDE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.302"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHU0J_004029"
     CDS             <1..197
                     /locus_tag="ACHU0J_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1218463.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHU0J_004030"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHU0J_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR1218464.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHU0J_004031"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHU0J_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR1218465.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..>2547
                     /locus_tag="ACHU0J_004032"
     CDS             1821..>2547
                     /locus_tag="ACHU0J_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR1218466.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLH"
BASE COUNT          607 a          606 c          658 g          676 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcaca
//
LOCUS       DAZPDE010000297         2545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPDE010000297 DAZPDE010000000
VERSION     DAZPDE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.303"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            131..466
                     /gene="eutS"
                     /locus_tag="ACHU0J_004033"
     CDS             131..466
                     /gene="eutS"
                     /locus_tag="ACHU0J_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1218467.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            479..958
                     /gene="eutP"
                     /locus_tag="ACHU0J_004034"
     CDS             479..958
                     /gene="eutP"
                     /locus_tag="ACHU0J_004034"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1218468.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            933..2306
                     /gene="eutA"
                     /locus_tag="ACHU0J_004035"
     CDS             933..2306
                     /gene="eutA"
                     /locus_tag="ACHU0J_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1218469.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
BASE COUNT          611 a          638 c          716 g          580 t
ORIGIN      
        1 attcataaca gtaaatatct ctccattctt attgatctac ctcacctttt aaacgcgctt
       61 gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag tttttcacag
      121 gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca aacaggtcac
      181 actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg gcgttccgga
      241 tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga tcgccggtga
      301 tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca gcggtgcatt
      361 ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg tcagcggttt
      421 aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg aggcattcat
      481 gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat ttaatgcgtt
      541 gcaggggaat tataccctcg ccagaaaaac actggccgtg gaatttaatg ataagggcga
      601 tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct taattaccac
      661 gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg aaagtcggtt
      721 gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca tcagtaaaac
      781 ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga cgggtttcga
      841 agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac tggtggatta
      901 tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca gcgaatgata
      961 ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc gccagcatta
     1021 ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc gaatgtgatg
     1081 aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa accgaaagcc
     1141 agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc gaaagcctgg
     1201 tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctgggcgat tttgtcgtcg
     1261 ccagcgccgg gccgcacctc gaatccgtga tcgccggtca cggagccggg gcgcaaaccc
     1321 tttctgaaca acggctgtgt cgggtactga atatcgatat cggcggtggc accgcgaact
     1381 acgccctgtt cgatgccgga aaaatcagcg gcactgcctg tctcaacgtc ggtggtcgcc
     1441 tgctggaaac cgacagccag gggcgcgtgg tttacgctca taaaccgggg cagatgattg
     1501 tggatgagtg cttcggtgca ggcactgacg cccgttcgct gaccggcgcg cagctggtgc
     1561 aggttacccg gcggatggcg gcgctgattg tcgaagtgat tgacggaacg ctttcgccgc
     1621 tcgcgcaggc attgatgcaa accggtttgc tgcccgcagg tgttacgccc gaaatcatta
     1681 cgctttctgg aggcgtgggc gaatgttatc gccaccagcc cgccaacccg ttctgttttg
     1741 ccgatattgg cccacttctg gcaacggcgc tgcatgacca tccgcgcctg cgtgagatga
     1801 atgtgcagtt tccggcgcaa accgtacgct ccacggtgat tggcgcgggt gcacataccc
     1861 tttcgctctc tggcagcaca atctggctgg agggcgtaca actgccgctg cgcaatttgc
     1921 cggtggcgat cccgattgat gaaacggatc tggtgagtgc ctggcaacag gcgctgcttc
     1981 agctggatct tgatcccaaa actgacgtgt acgtgctggc gcttcccgcc tcgctgcctg
     2041 tgcgttacgc cgcggttctg acggtcatca acgcgctggt cgatttcgtc gcgcgttttc
     2101 cgaatccgca tcccctgctg gtggtggccg ggcaggactt tggtaaagct ctgggcatgt
     2161 tgttgcgccc acagctacaa caactcccgt tggcagtcat tgacgaagtg attgtccgcg
     2221 cgggggacta tatcgacatt ggtacgcctc tttttggcgg atcggttgtg ccggtgacgg
     2281 tgaaatcact cgcatttcct tcctgaatac ggtagttatc aggtatggtg cttggtgtct
     2341 gggtgttagt gggattatat ccggcagtgt atccggttaa ggtgattggt ctggcaagat
     2401 gattggatgc ttttgtggtg aaaaatgttt tggtgtttta gcataaccct gaagaaagaa
     2461 taaaaataaa aaggaaaaaa ccaaaaccag aacagaaccg gaaagagaag aaaagagaaa
     2521 atgaaaaata aaaaccaaaa aagaa
//
LOCUS       DAZPDE010000298         2491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPDE010000298 DAZPDE010000000
VERSION     DAZPDE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.304"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>2491
                     /locus_tag="ACHU0J_004036"
     CDS             <1..>2491
                     /locus_tag="ACHU0J_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1218470.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZPDE010000299         2489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPDE010000299 DAZPDE010000000
VERSION     DAZPDE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.305"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..567
                     /locus_tag="ACHU0J_004037"
     CDS             <1..567
                     /locus_tag="ACHU0J_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218471.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="ACHU0J_004038"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="ACHU0J_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1218472.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACHU0J_004039"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACHU0J_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1218473.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="ACHU0J_004040"
     CDS             2169..2405
                     /locus_tag="ACHU0J_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1218474.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          639 a          546 c          644 g          660 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta aggacacta
//
LOCUS       DAZPDE010000300         2463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPDE010000300 DAZPDE010000000
VERSION     DAZPDE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.306"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(746..2350)
                     /locus_tag="ACHU0J_004041"
     CDS             complement(746..2350)
                     /locus_tag="ACHU0J_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1218475.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          575 c          526 g          714 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagtagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DAZPDE010000301         2450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPDE010000301 DAZPDE010000000
VERSION     DAZPDE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.307"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACHU0J_004042"
                     /pseudo
     CDS             complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACHU0J_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
     gene            complement(1245..2234)
                     /locus_tag="ACHU0J_004043"
     CDS             complement(1245..2234)
                     /locus_tag="ACHU0J_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218476.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2318..>2450)
                     /locus_tag="ACHU0J_004044"
     CDS             complement(2318..>2450)
                     /locus_tag="ACHU0J_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218477.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          653 a          560 c          589 g          648 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctgag ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg agcctacgac aaatcgaaga
      841 caaccagcta acctattgtt tcttaaagac aggtgaatcc agttggttgt agtgatagtc
      901 acacatttag agatagaagg tcaagacttg aagcacgtaa tattccctct aaaggaatag
      961 aactcacggt ctgactaccc ggcccttttg gtctggctca ggtcaggcta tgggggcttt
     1021 tggctcgctc tttatattag atgaggtctc acatttttca gtcaaaactc atcgtctaat
     1081 agcccatagc cagaccacca gtcccagctt tagcaggaag accacgagat agactttgtg
     1141 taggtccatt tggccctcct actttctgta ttcacaaact agattactag tacaaaataa
     1201 aggccagaca tccagttcgg aagtctgacc aatagtcgtt attgttagtt agcttcttta
     1261 gtgaagctca ggcgtttgac tacaggcagg acagcagtag ccgagccagt acccatcttg
     1321 atggtcgtag cagcttcagc tacaacaaac accttgccga gaatctcaag gtcgtgagac
     1381 tgggagtctc catcacgtga aaggaccaca gtaatgctct cgccatcgtg acggaagcaa
     1441 ccgaagctca gagccgtaaa acgcttagcc atcatgaaca cgccacggtc ttcaccgttg
     1501 taataggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc gaagccctgc
     1561 ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat gccgtagtca
     1621 gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat gatagcgtca
     1681 gccttagcgg tcaggctacc agtcagggcc ggaagttcta cggtcttcag cagctttgag
     1741 gactccagca cgtcaaccat cttagagtta atggtacgaa tcacgttatc gcagatgttc
     1801 agtgcgtcct gctggagcgt cccctgagtg aaaccagtgg tcgacttctg gtcccaaagc
     1861 tttgtttggt gagacacgag gatcgcctgt tcaatcggct caacggtgat ttcagtgcca
     1921 gacggagcgg tcacaacttc aggtcgctta ccaccacgga cagggaccag cacagagcgg
     1981 tcagccagag tgtgagcaat gattggagtc ggtaagctgt tacgggtcgg caggacttct
     2041 acagggaacg ggatgttatc ttgagcgtct ttaagtacac cgttcagcat agccttaaca
     2101 gtcttagcta actggagggc atagacctga gggtcggcgt tagtcggcag accttcattg
     2161 acctgaatgt tgtcggtatc ggtgatgcgt actggctcaa tgccaaactg gaaagcgttt
     2221 ttagtcatat tcattataat taatctcctt gagtcaaata cttgaagtcc ctaacaggca
     2281 gtcagggacc gtgtgttctc agatgaggga cgcttgctta caagctgtcc atccagttac
     2341 cgcctgactc aaggtcagcg acgatagcgg cacgtgagcg accagcggaa tgagtagcgc
     2401 tgcggaggtt ttcaccatag gtccggcgtt cagctttgac ctgttcgcgg
//
LOCUS       DAZPDE010000302         2416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPDE010000302 DAZPDE010000000
VERSION     DAZPDE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.308"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHU0J_004045"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHU0J_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR1218478.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHU0J_004046"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHU0J_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1218479.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHU0J_004047"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHU0J_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHU0J_004048"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHU0J_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          600 a          623 c          653 g          540 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 ggtatcataa acatgcttga caacttaatt agggccccct cttcctttta tggtgtaatt
     2281 gtttgattga atacttccgg ggatttcagt atgaaatgaa gtatatttaa tatactttac
     2341 aaaaagtttg tattagtgga ataaggacaa tagcttatcc aaactgccaa gcctctttat
     2401 tataagtaag atctgg
//
LOCUS       DAZPDE010000303         2387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPDE010000303 DAZPDE010000000
VERSION     DAZPDE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.310"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHU0J_004049"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHU0J_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1218480.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHU0J_004050"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHU0J_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1218481.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHU0J_004051"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHU0J_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1218482.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2387
                     /locus_tag="ACHU0J_004052"
     CDS             1993..>2387
                     /locus_tag="ACHU0J_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218483.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITD"
BASE COUNT          619 a          561 c          585 g          622 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagtctatca cagattt
//
LOCUS       DAZPDE010000304         2371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPDE010000304 DAZPDE010000000
VERSION     DAZPDE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.311"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..823)
                     /locus_tag="ACHU0J_004053"
     CDS             complement(<1..823)
                     /locus_tag="ACHU0J_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218484.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="ACHU0J_004054"
     CDS             complement(1422..1670)
                     /locus_tag="ACHU0J_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1218485.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1933..2028)
                     /locus_tag="ACHU0J_004055"
                     /pseudo
     CDS             complement(1933..2028)
                     /locus_tag="ACHU0J_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2025..>2371)
                     /locus_tag="ACHU0J_004056"
     CDS             complement(2025..>2371)
                     /locus_tag="ACHU0J_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1218486.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          436 c          574 g          750 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaatac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg g
//
LOCUS       DAZPDE010000305         2334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPDE010000305 DAZPDE010000000
VERSION     DAZPDE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.312"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHU0J_004057"
     CDS             <1..389
                     /locus_tag="ACHU0J_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1218487.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="ACHU0J_004058"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="ACHU0J_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1218488.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="ACHU0J_004059"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="ACHU0J_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1218489.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLSLYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          584 a          460 c          510 g          780 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc tttcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata accc
//
LOCUS       DAZPDE010000306         2326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPDE010000306 DAZPDE010000000
VERSION     DAZPDE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.313"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHU0J_004060"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHU0J_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1218490.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHU0J_004061"
     CDS             282..452
                     /locus_tag="ACHU0J_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR1218491.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHU0J_004062"
     CDS             527..802
                     /locus_tag="ACHU0J_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218492.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..2317
                     /locus_tag="ACHU0J_004063"
                     /pseudo
     CDS             902..2317
                     /locus_tag="ACHU0J_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          720 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggtag
//
LOCUS       DAZPDE010000307         2261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPDE010000307 DAZPDE010000000
VERSION     DAZPDE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.314"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="ACHU0J_004064"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="ACHU0J_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1218493.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="ACHU0J_004065"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="ACHU0J_004065"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1218494.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="ACHU0J_004066"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="ACHU0J_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1218495.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          503 a          594 c          537 g          627 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac atcggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac a
//
LOCUS       DAZPDE010000308         2246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPDE010000308 DAZPDE010000000
VERSION     DAZPDE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.315"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(158..670)
                     /locus_tag="ACHU0J_004067"
     CDS             complement(158..670)
                     /locus_tag="ACHU0J_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1218496.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            865..1629
                     /locus_tag="ACHU0J_004068"
     CDS             865..1629
                     /locus_tag="ACHU0J_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218497.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1580..>2246
                     /locus_tag="ACHU0J_004069"
     CDS             1580..>2246
                     /locus_tag="ACHU0J_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1218498.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          645 a          489 c          565 g          547 t
ORIGIN      
        1 atcatcagcg accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt
       61 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
      121 ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      181 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      241 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      301 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa
      361 cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa
      421 ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga
      481 ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa
      541 ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa
      601 aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc
      661 actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt
      721 aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag
      781 cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa
      841 cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca
      901 taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg
      961 gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc
     1021 aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt
     1081 acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga
     1141 atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc attaaaacac
     1201 ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg
     1261 acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt
     1321 ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt
     1381 gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc
     1441 gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg caaggccgcc
     1501 actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat tgatgatggc
     1561 aatggagagc gtgacctcaa tgacttctac tctgacatcc aaaccgacgc tgaatccggc
     1621 tttacgtagt ttctggacta tgcgggcacg taacaaagtg ctttatggtg gtcggtcatc
     1681 gtcaaaatca tgggatgccg ctggcattgc catatttctg tcgaataaat acaccctgcg
     1741 tttttgttgt gcccgtcaga tccagaataa aatcgaagag tcggtgtata ccctgctcaa
     1801 aattcagata gacaggtttg gtctgcggca ccgtttccgt attctgaaca acaaaatcat
     1861 taaccgggtt actggctcgg aatttgtttt ttatggatta tggcgcaaca tcgaagaaat
     1921 taagtcactg gaggggatcg atatgttgtg gttggaagaa gcccacgcac tgacggaata
     1981 ccagtggaaa attctggagc caacgatccg taaagagggt tcggaatgct ggttcatatt
     2041 caaccccgga cttgttactg atttcgtctg gcgcaacttc gttgttgatc cgcccgaagg
     2101 cactctcatc cgcaaaatta actatgacga aaatccgttt ctgtctgaca ccatgcttaa
     2161 ggttatcgac gcggcgcgac gccgtgatcc ggatggtttt aaacatgtgt atgagggcgt
     2221 tccggagtct gatgatgatg cggcaa
//
LOCUS       DAZPDE010000309         2241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPDE010000309 DAZPDE010000000
VERSION     DAZPDE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.316"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            67..585
                     /gene="mddA"
                     /locus_tag="ACHU0J_004070"
     CDS             67..585
                     /gene="mddA"
                     /locus_tag="ACHU0J_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1218499.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            582..1031
                     /locus_tag="ACHU0J_004071"
     CDS             582..1031
                     /locus_tag="ACHU0J_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1218500.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1032..1265)
                     /gene="ydcY"
                     /locus_tag="ACHU0J_004072"
     CDS             complement(1032..1265)
                     /gene="ydcY"
                     /locus_tag="ACHU0J_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1218501.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1351..1524)
                     /gene="ortT"
                     /locus_tag="ACHU0J_004073"
     CDS             complement(1351..1524)
                     /gene="ortT"
                     /locus_tag="ACHU0J_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1218502.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1719..1814
                     /gene="yncL"
                     /locus_tag="ACHU0J_004074"
     CDS             1719..1814
                     /gene="yncL"
                     /locus_tag="ACHU0J_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1218503.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          555 a          507 c          538 g          641 t
ORIGIN      
        1 ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg
       61 ccagacatgt ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat
      121 aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac
      181 cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa
      241 gatggcgtag ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc
      301 cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt
      361 aaattgttaa gtcgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc
      421 gggatcgaat cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc
      481 accgcacaaa tgccgcaggt aggcactaaa tttggtcatt ggctggatct gacatttatg
      541 cagttgcaac tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc
      601 ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggcgcgca
      661 tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg
      721 tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt
      781 ccagcgtgcg ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca
      841 gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc
      901 agctgattgg cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt
      961 tgttcggccc aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac
     1021 gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcg cgcttccata
     1081 tcttctttat gcttgcgacc gtgatgagca atagcggtgc gcaaacgctg ctgctgggtg
     1141 tagcgatctt cccgacttaa ctccgcgtca tcgctcaggg cgattaataa ttcgttcata
     1201 tgggcaatga cgctctcttc aatcgcggca tccacgcgcg cgatgacttc atccagatgc
     1261 gacatacttt ctccttagcc acttgtgaaa tttatcgatt aatccaaatg atataagtct
     1321 gcgtggtgcc gtcaagccag tgagtaagat ttaaaacagt gaaaacaaca gcgccaccgg
     1381 gaaactcatc ggccatgtgg cccccaccag aaaggcgctt aagaaacgga tgcgtttctt
     1441 atcgtgagaa agaaaccagg tgataagaaa tgcgattgct gccataaccg cataaaaaac
     1501 aagcatatgt tgatagagaa acatgtacgg gcgtccagcc atatgaataa aacgcgcgca
     1561 aatatgcgcc attttttgac gtgcatcaag ttttttcacc agtttgagaa aaaatcatca
     1621 aacagcaact tgcccttatc catcttcaga atgagtatta ttgcggattt tcttaacccg
     1681 tactatacac ataggggcaa tgataaaagg tggcaaaaat gaatgtttcc agtagaactg
     1741 tagtactgat aaatgtcttt gctgctgttg gtttgtttac tcttatctct atgagatttg
     1801 gctggtttat ttgatgtagt gattttgcac gcagcacgta ctgtcagaga aatcaactct
     1861 ctgacagtac gcaagatctt ctcatactct ccaagtttgc aacattatcc ttaatattta
     1921 cttctttttg ctggcatcta cattgaaatg agtagaaaac atccccctac tgtcgtctat
     1981 acaaacagct tactgattca aacggaatct tgttttcaca tttttctatc acgaatactt
     2041 tatacgcgcg ggagtctata aatgctttat actttcaagg aataacgtaa atataaaaag
     2101 atggcgtaaa gtatatgcat ggcaggatgt ttgcaacctc cttgatgacg ctgccccatt
     2161 gaaattgcaa accccgccct ggttatcttg cccatttatc atgcaaaaca gataattata
     2221 ggcctctgaa tacctcagaa a
//
LOCUS       DAZPDE010000310         2218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPDE010000310 DAZPDE010000000
VERSION     DAZPDE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.317"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHU0J_004075"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHU0J_004075"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1218504.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHU0J_004076"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHU0J_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1218505.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHU0J_004077"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHU0J_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1218506.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZPDE010000311         2202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPDE010000311 DAZPDE010000000
VERSION     DAZPDE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.318"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..331)
                     /locus_tag="ACHU0J_004078"
     CDS             complement(<1..331)
                     /locus_tag="ACHU0J_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218507.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACHU0J_004079"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACHU0J_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1218508.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACHU0J_004080"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACHU0J_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1218509.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACHU0J_004081"
     CDS             complement(1692..2072)
                     /locus_tag="ACHU0J_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR1218510.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..>2202)
                     /gene="yigG"
                     /locus_tag="ACHU0J_004082"
     CDS             complement(2086..>2202)
                     /gene="yigG"
                     /locus_tag="ACHU0J_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR1218511.1"
                     /translation="TMIAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT          570 a          524 c          514 g          594 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gt
//
LOCUS       DAZPDE010000312         2188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPDE010000312 DAZPDE010000000
VERSION     DAZPDE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.319"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACHU0J_004083"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACHU0J_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACHU0J_004084"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACHU0J_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1218512.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACHU0J_004085"
     CDS             complement(1288..2046)
                     /locus_tag="ACHU0J_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1218513.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          656 a          484 c          455 g          593 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt catttttt
//
LOCUS       DAZPDE010000313         2187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPDE010000313 DAZPDE010000000
VERSION     DAZPDE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.320"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHU0J_004086"
     CDS             complement(220..384)
                     /locus_tag="ACHU0J_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218514.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHU0J_004087"
     CDS             1286..1570
                     /locus_tag="ACHU0J_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1218515.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHU0J_004088"
     CDS             1558..2043
                     /locus_tag="ACHU0J_004088"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1218516.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZPDE010000314         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPDE010000314 DAZPDE010000000
VERSION     DAZPDE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.321"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHU0J_004089"
     CDS             <1..203
                     /locus_tag="ACHU0J_004089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218517.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHU0J_004090"
     CDS             293..694
                     /locus_tag="ACHU0J_004090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218518.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHU0J_004091"
     CDS             696..1301
                     /locus_tag="ACHU0J_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR1218519.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHU0J_004092"
     CDS             1288..>2183
                     /locus_tag="ACHU0J_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1218520.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZPDE010000315         2172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPDE010000315 DAZPDE010000000
VERSION     DAZPDE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.322"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            20..523
                     /locus_tag="ACHU0J_004093"
     CDS             20..523
                     /locus_tag="ACHU0J_004093"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1218521.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(569..985)
                     /gene="rraB"
                     /locus_tag="ACHU0J_004094"
     CDS             complement(569..985)
                     /gene="rraB"
                     /locus_tag="ACHU0J_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1218522.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1147..2160
                     /gene="argF"
                     /locus_tag="ACHU0J_004095"
     CDS             1147..2160
                     /gene="argF"
                     /locus_tag="ACHU0J_004095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1218523.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          557 a          523 c          540 g          552 t
ORIGIN      
        1 atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct
       61 gacgctgcag gataatcctg ctatcgccag cgtcatccgt caggtttacg cagagtatgg
      121 tcttaccgcc gataaaggct acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt
      181 gtacagccag cctggccatg cttattgggt ggttgaatac aatggcgaag tggtcggcgg
      241 cggcgggatt gcgccattaa caggtagtga agcggacatt tgcgaactgc agaaaatgta
      301 ctttcttccc gctattcgcg gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga
      361 gcaggcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt ttttaaagga
      421 agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcgc ttggctgcac
      481 gggccatgtc gattgtgaag tgcggatgct acgcgaactc taaaaaaatg cccgctagta
      541 tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg tcttcatcga
      601 caaaatcttc atcgtcgcca tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt
      661 cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg gcatcgatca
      721 gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata tcaccatctt
      781 caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct tcaactgccg
      841 ctttttccag agtttccaga tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt
      901 ccggatcgct gccatcttcc agtaattctt caataatcaa acgtgtttct tcacgctgtt
      961 cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc gatactctta
     1021 ttgtcacaca ccactgacat tgcctccacc tttgtgacaa agatttatgc tttagacttg
     1081 caaatgaata ataatacaca taaattgagt tttaattcat tgaggcgtta gccacaggag
     1141 ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt cacgccagct
     1201 gaactcaaca gcctgctgca gttagccgcg aagctgaaag ctgataagaa aagcggtaaa
     1261 gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt
     1321 acccgatgct ctttcgaagt tgccgcatat gaccaggggg ctcgcgttac ttatcttggc
     1381 ccaagcggta gtcagattgg tcataaagag tcgattaaag acactgcccg cgtgcttggt
     1441 cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgtcga aacactggcg
     1501 gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc cacgcagttg
     1561 ctggcggatc tcctcaccat gcaggagcat ttacccggta aagcattcaa cgaaatgacg
     1621 ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga agctgcggcg
     1681 cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccaga agctgcgctg
     1741 gttacggaat gccgcgccct ggcacaacaa aatggcggga atattacgct gacggaagat
     1801 gtagctaaag gagttgaagg tgctgacttt atctataccg atgtatgggt gtcgatgggt
     1861 gaagcaaaag aaaaatgggc tgaacgcatt gcattactgc gtgattatca ggtgaacagc
     1921 aagatgatgc agttgaccgg taacccggag gtcaaattcc tccattgcct gcccgcgttt
     1981 catgacgacc aaacgacgct tggcaaaaaa atggcggaag agtttggcct acatggcgga
     2041 atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtatttga tcaggcggaa
     2101 aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa
     2161 tatctctata aa
//
LOCUS       DAZPDE010000316         2167 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPDE010000316 DAZPDE010000000
VERSION     DAZPDE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.323"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..821
                     /locus_tag="ACHU0J_004096"
     CDS             <1..821
                     /locus_tag="ACHU0J_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1218524.1"
                     /translation="FITLLVNADLSVVEMVFIIMKNFNVLTKVVFCLSFLSSAFPTYA
                     ADDVKVTQNTSVFKLKLSATRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAA
                     PFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKI
                     AENVNLNIQVNVHTCIKLITRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLS
                     SLQVGGSKIEKLDYIAPFSQHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            895..2167
                     /locus_tag="ACHU0J_004097"
                     /pseudo
     CDS             895..2167
                     /locus_tag="ACHU0J_004097"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          626 a          391 c          524 g          626 t
ORIGIN      
        1 cgttcattac cttgttagtg aacgcggatt tatcagttgt cgaaatggtt tttataataa
       61 tgaaaaattt taatgtacta accaaagtcg tattttgctt gtcgttttta tcaagcgcat
      121 ttcccaccta tgctgcggat gatgtcaaag tgactcaaaa cacttcagtt tttaaactga
      181 agcttagtgc aacgcgatta atctatgaac aagggtcgga tggcgcagcg ataactgtga
      241 caaatccgca ggattggccg atgcttgtgc agagtaaagt ctttgaagaa gataaagtaa
      301 ctgcagcgcc ctttattgtc actccgccac tgttcaggct taatccaggc cagcaaaata
      361 aaatgtgggt tgtgcagact gcgccagctt tacaagacag ggaaaaatta cactggctat
      421 gcgccaccgg cattacgcct aaaaagagtg atgtttgggc cgagaaagat aaagaaaaaa
      481 ttgctgaaaa tgtgaacctg aacatccagg ttaacgtaca tacctgcata aaacttatta
      541 ccaggccatc tggtattgaa ggcaagatgg ttaatgcgag tgagaagtta tcgtggaaaa
      601 agcagggtga taaaattgtc gcggtcaatc agtcgccgtt ctacatcaac ctttcttctt
      661 tacaggttgg cggtagcaag attgagaaac tagattatat tgcgccgttt tcgcaacatg
      721 agtttgttat gccaaaaact agcggccagg atattaaatg gtctgtgatt actgatgaag
      781 gtggtgagag tcgtttgttt atatctcatt tgcaatcata atgtattaca tttttattga
      841 atatttttat gccatgcata ttccatgttt tatcgactgt cattaatttt gataatggcc
      901 ttacttgcgg gtcagttaag tgcccaggag tggtcatttg attcttcaca gttggaagga
      961 aacgttagtg cggacactgt ggcaatgttt aatcagggag agcaactccc cggaaattat
     1021 cgtgtggaaa tttatcttaa cggtgaaaaa gttgatgttg gggagtttcc ctttcatcgt
     1081 ccggaatccc ctgaagaaaa agagcttgtt ccatgtttaa ccgttgatga ccttatccac
     1141 tatggcatta aaattgataa aagcagcagt gatactgaca ataaaaagaa tcagtgtttt
     1201 aaatggaact ctattgaagg tttgaaagta aattatgatt ttgatagcca gcgcgtacag
     1261 ataaccgttc cacaactgta tctgcaagat aaaaaaagta gtttagcacc agtgtcgtta
     1321 tggaatgagg gtgtagctgc attccgcatg gtgtatcaga caaatattga tatttccaaa
     1381 caaaatgata atcaatctac cacgcgtaat tctcgttatg gcaggttcac tccagggttt
     1441 aatcttggtg cctggaggtt tcgtagttca gtgacatgga gcaaggaact tggccagtca
     1501 gaaagatggc aaaggggtta catgtggttt gagcgaggca taaatgcgat taaaagccga
     1561 ttaacgcttg gagaatctta tacgtcgtct gaggtgtttg acagcatacc ttttcgcggc
     1621 gggatgttag cgaccgacga tgcgatgact ccgccggagg acagctacta cacacctgtg
     1681 gtgcatggaa tagcacaaag tgaggcgcag gttattatta aacaaaatgg gcaaattatt
     1741 tttacccgtt ctgtgccgcc agggcctttt gcgctggata atttaccaac gctggccgtt
     1801 ggcggggagc ttgatgttac agtgagagag agtaatggcg aggagcagta tttttccgtg
     1861 ccatttcaaa cgccggccat cgctctgcat gaaggatatt tcaaatatag tgtgatgggg
     1921 gggaatataa agaagaaggt ataaaaagct cccctgtctc cgtgggcgaa atcacggcga
     1981 tgtacgggct tccttatggc gtcacggtgt atggagggat tcaaaacgcc actcatttta
     2041 atgcaatatc tacgggtatt ggcatttctc tggggctgtt agggagttta tcgacagata
     2101 ttacgcgttc gattgcgaat ttatattatg ggaataaata tcgtattcgt tatagtaaat
     2161 ccattag
//
LOCUS       DAZPDE010000317         2164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPDE010000317 DAZPDE010000000
VERSION     DAZPDE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.324"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHU0J_004098"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHU0J_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR1218525.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHU0J_004099"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHU0J_004099"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR1218526.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..>2164
                     /locus_tag="ACHU0J_004100"
     CDS             1849..>2164
                     /locus_tag="ACHU0J_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1218527.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          587 a          482 c          511 g          584 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atct
//
LOCUS       DAZPDE010000318         2125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPDE010000318 DAZPDE010000000
VERSION     DAZPDE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.325"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..128)
                     /locus_tag="ACHU0J_004101"
     CDS             complement(<1..128)
                     /locus_tag="ACHU0J_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR1218528.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDETFSV"
     gene            complement(67..390)
                     /locus_tag="ACHU0J_004102"
                     /pseudo
     CDS             complement(67..390)
                     /locus_tag="ACHU0J_004102"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(387..920)
                     /locus_tag="ACHU0J_004103"
     CDS             complement(387..920)
                     /locus_tag="ACHU0J_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1218529.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(986..1615)
                     /locus_tag="ACHU0J_004104"
     CDS             complement(986..1615)
                     /locus_tag="ACHU0J_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218530.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1619..1834)
                     /locus_tag="ACHU0J_004105"
     CDS             complement(1619..1834)
                     /locus_tag="ACHU0J_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1218531.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          545 a          564 c          468 g          548 t
ORIGIN      
        1 accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc
       61 ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg
      121 accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc
      181 cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat
      241 ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt tgtaggtaat
      301 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
      361 agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc taatgcgctt
      421 tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc
      481 ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc
      541 agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat
      601 gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg ctccacccat
      661 gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt cgacagcttc
      721 attcccggta tgacaggctt accatccacc attgtggcac cacgacagat ggtccatatg
      781 ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag aaactggtca
      841 agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc cgacaggccg
      901 tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa taacgagcca
      961 acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc aggctctttc
     1021 ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca tccatgtctg
     1081 caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg caacggccaa
     1141 tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga ttaataattt
     1201 tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg acgaactcac
     1261 cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc ggtgcagacg
     1321 caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg acgtactcag
     1381 gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt tcctgcaaaa
     1441 acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc gcataatgct
     1501 catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt ttattaagct
     1561 cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc ttcattattt
     1621 actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga ttcgtcagat
     1681 aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac tgtgatggcc
     1741 tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag gtgccgtagg
     1801 caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa ataacggatg
     1861 gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca aaagcgggcg
     1921 aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag ttaatcggaa
     1981 taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct gcacagcaat
     2041 tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc gggatttccc
     2101 atcatcgcag actgaaagac tctga
//
LOCUS       DAZPDE010000319         2098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPDE010000319 DAZPDE010000000
VERSION     DAZPDE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.326"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..357
                     /locus_tag="ACHU0J_004106"
     CDS             <1..357
                     /locus_tag="ACHU0J_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR1218532.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACHU0J_004107"
     CDS             619..981
                     /locus_tag="ACHU0J_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR1218533.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACHU0J_004108"
     CDS             978..1907
                     /locus_tag="ACHU0J_004108"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1218534.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2098
                     /locus_tag="ACHU0J_004109"
     CDS             1904..>2098
                     /locus_tag="ACHU0J_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR1218535.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLP"
BASE COUNT          593 a          424 c          473 g          608 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccct
//
LOCUS       DAZPDE010000320         2092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPDE010000320 DAZPDE010000000
VERSION     DAZPDE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.327"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHU0J_004110"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHU0J_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1218536.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHU0J_004111"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHU0J_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1218537.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZPDE010000321         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPDE010000321 DAZPDE010000000
VERSION     DAZPDE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.328"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHU0J_004112"
     CDS             complement(60..1025)
                     /locus_tag="ACHU0J_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1218538.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHU0J_004113"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHU0J_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218539.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          578 a          549 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga acccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPDE010000322         2086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPDE010000322 DAZPDE010000000
VERSION     DAZPDE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.329"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..798
                     /locus_tag="ACHU0J_004114"
                     /pseudo
     CDS             1..798
                     /locus_tag="ACHU0J_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="ACHU0J_004115"
                     /pseudo
     CDS             913..1254
                     /locus_tag="ACHU0J_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="ACHU0J_004116"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="ACHU0J_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          505 c          631 g          435 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagc
//
LOCUS       DAZPDE010000323         2081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPDE010000323 DAZPDE010000000
VERSION     DAZPDE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.330"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(124..858)
                     /locus_tag="ACHU0J_004117"
     CDS             complement(124..858)
                     /locus_tag="ACHU0J_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1218540.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            complement(858..>2081)
                     /locus_tag="ACHU0J_004118"
     CDS             complement(858..>2081)
                     /locus_tag="ACHU0J_004118"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1218541.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
BASE COUNT          583 a          538 c          448 g          512 t
ORIGIN      
        1 atttttttcc aggtttcaca gctacaaacc cgtggcaaaa catagcaccc atctattact
       61 aacctcacca cgcatgctca gcgatttcgt catcgaaaaa gagagttatc tatgacgcat
      121 caattatact ttttgctttg ttcgcatatt gttacatctt tcataccttg acgcatctct
      181 tcccaacgcg ggtatcgcct gttgtataag ttgagcatta cgtactaacc agacgtactc
      241 acttttattc ctggtgccta atcgttctcg aattgcggac tcatgggcgc taacggtctt
      301 gaggccaata ccacagattt ctgcaatttt acgtgtgcta atccccttcg cgcggagtaa
      361 aagaaccctc cattgcactc ttcccaggcg ttttataaac aaaggaccag gcaaacaatg
      421 agcatttaac accttccgta atgaagataa taaaatatcc acttttgcac tcccatgagt
      481 aagccatact cgggaaagag gcccgatatc attcgtcatt ccttcaggaa tagcatcgct
      541 aataacgagt tgccgcatca gacggtatcg acgacaaacc ttcacaatat gtccggctaa
      601 ttcaggcaaa ccatcgtgac gcatttttac ccatatcacg atgctactgt cagtcagaac
      661 attcatatca gtgggtaagg aagtgaaaac ctgaagtttc actcccccaa cacgcttgag
      721 caaactgcac acgccgcaaa tggcaaaaag atccaggcca atcaccgtga catttgtcca
      781 tgtggtattt tctggcgaga gaatattaca gttgatgttt gtgctttcta atttcttata
      841 cgtcagatcg ttaaccatta gtttcccccg cgagtaaaac gttcgtcatt tacaaatacc
      901 cgagctgcgc gttttcttat tttttcagct gtcagataat caccgcgcct gtgcgcaatg
      961 aatatcattg cagcgcttac gtctgcatca ttgtggattg ccaaccattt atttccccac
     1021 acagtgaaaa gagacatgct ttccgtatcg cgaacgaacc ccggtttatt cgcaataagc
     1081 agatgctgat cgcgctcaag acgttctgcc tgcgtcaggt atttccagct atgttccgga
     1141 aggcgaggga taaatccttc gttcatatca gcttcagctc tggtcagttc ccgctgtgca
     1201 tcaaatcccg ctaccaggat gaccagcgcg gcggtcataa tgagcacaat gacaggcaga
     1261 atacccaacc ggactgtttt aggtatacgt acctgattca tcacgttttc cggcattccg
     1321 atgcggaaaa ggagcagcaa cagcatcaaa tggggaacag acgtatagag aggatattcg
     1381 gtcatcatat gaatgactaa aggcaacccc attaaggcaa taaaccactt ttcacattga
     1441 tggtgcgcgt tattgtttgt cattttcagg cggcatatac acggccacag ccaaacgcct
     1501 gatagcagca aaaaaccaaa caaggcgacg atgccacctt cagccgttac gaacagcagt
     1561 tcgttatggg gatgcgtagc cgtatcactc tctatgtttg tcacgcccac atcctctaag
     1621 gcttgcggga aggtcgcttc aaagctgcct aacccccaac cggttaaggg gcgctgttga
     1681 ataagactta tggcaccttt cagaatacgt atacgctggt cattcgatga tgaatgcgta
     1741 aagtcagccg ttgtctcccc tggcatggca tagtttgaga taccgacaca ccagcacagc
     1801 aaggaggcga cgaagatcaa gcgcaacaga ccgctaaggg gacgtccgcg ccagcggggt
     1861 ctgtccgcga gagcggccag cacgatgacg caaaataaac caagaaggcc gacacggctt
     1921 tgtgtaagga ctaaagctgt agacagaggg tataacgaca acaaacatat caacgtcaga
     1981 gaacgagtac gcgccgttaa agccagatac aacgctacgc cgcaacctgt tgccagccag
     2041 ctcgcaagaa catttcgctg ctggaaaatg ccatacggaa a
//
LOCUS       DAZPDE010000324         2077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPDE010000324 DAZPDE010000000
VERSION     DAZPDE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.331"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..90)
                     /locus_tag="ACHU0J_004119"
     CDS             complement(<1..90)
                     /locus_tag="ACHU0J_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218542.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLG"
     gene            complement(217..1866)
                     /locus_tag="ACHU0J_004120"
     CDS             complement(217..1866)
                     /locus_tag="ACHU0J_004120"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1218543.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          501 a          547 c          528 g          501 t
ORIGIN      
        1 tcccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg
       61 tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac
      121 tggatcggtc aactgatcct taactgatcg gcattactcc tcctgaagag gataatttta
      181 cagccagcag gaagttagcg gctggctgta aaaatattat ggttcgccgt acaaaccaat
      241 taaacggcgt accacaccgt tagtccagcc aaacccatcc tgcaacggat actcgccacc
      301 gccgccttcg cggggaacac catcggcaat atggtatttc tcgatcattt tgtgctgttc
      361 cagatagaac tgattcaccg ttttcagcca gctgcgcgcg atttcatcac ccagaaggtc
      421 atcgccatac attttaaatc cctgaattgc catccattgt aacggtgccc agccgttggg
      481 tttatcccac tgttcaccgg tttcgtactc gcttgccaaa atcccgccag gtgtcagtaa
      541 ccggctgcgt acggcgtttg ccagacgatc ggcctgttca tggttcgcca tgccgacata
      601 gagcggcaca atggcggcag cggaaaacag cgccagttgt tcgcgtcgcc agtcgtaatc
      661 gcggtagatg ccgttttcat catcccagag gtaacggttt accgcatcgc gacgggcact
      721 ggccttctgg cggaacagcg cttctgtctc tttctcgcct ttcagcgccg agatgttggc
      781 gatggcgctc tccagtttaa acaggaaggc attcagatcg atggggatga actgggtggt
      841 acgaatgctc gccagacgac cagtatcacg cagccaacgg gaagagtaat cccaaccgga
      901 ggccgctccc gcgcgtaaat cgcggtacac ctcgttgggc gggcgaccag aatgtttcgc
      961 ggtttcaacg tcctcaagcc aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg
     1021 gttgagcagc gatccgtccg gcatccgcac aacatggcga taggcctgat tagggatcaa
     1081 cgattctgca ccgtccatcc agaaggcata ttccatttta aggtggtcga gatagcggcg
     1141 cgcaccgcgt acaccatctt cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg
     1201 cgagcggctc aaataatagg tgcggttgcc gttggggatg tgaccgtagt tttcgatcat
     1261 ccaggcgaag ttatcggcca tgcatttcag caaatcttcc cgaccacttt ccgccagtcc
     1321 cagcatggtg aaataggaat cccaatagta ggtttcgcta aaacggccgc ccgggacaat
     1381 atatgactgc ggtagcgcca gcagagaaga ccacggaatg tgatcctgtg gttcgcgggt
     1441 tagcaccggc cacagctggt cgatatgctc tttcagggaa ttttgcgggt ccgatacata
     1501 ctcgctggag tagacctccg gcagccagaa gtgattttca acaaacttgc gcaagtcaaa
     1561 atcacgatga cggcgcactt tacggtagcg gattaaaata tccagcgggt ccattttggg
     1621 tgcgcagtcg ggaaaggttt tactgtcggg gaaaattttc gccgactgaa catgctcgaa
     1681 cagttcgaga tagcgatcgg caggcgtcag cgcatcagag gcgggcagcc cttcaatcat
     1741 ctcgggttcc ggttctgcct cgatcatctc atccagtttt aattcatacg ggtccagttc
     1801 atagcagaga tcgacctcga tcatcagttc gtctggatta gggttttgaa ttttctgatt
     1861 gagcataagg atccaacctc cgaaattcgt cgctaattat cgggcttttc tttaagggta
     1921 gtcattcgct ccgcctggtg ggcgacaaag cgtgcggtgg atcacgttta ttaacttgtc
     1981 aaaaaatata acgcactaat atcaatggaa tttataaaaa tacggatttg gtgttgtttt
     2041 tcattttcag aacattcaat cctgctctcg ccgcaat
//
LOCUS       DAZPDE010000325         2048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPDE010000325 DAZPDE010000000
VERSION     DAZPDE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.332"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(95..171)
                     /locus_tag="ACHU0J_004121"
     tRNA            complement(95..171)
                     /locus_tag="ACHU0J_004121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Arg,seq:tct)
     gene            complement(273..348)
                     /locus_tag="ACHU0J_004122"
     tRNA            complement(273..348)
                     /locus_tag="ACHU0J_004122"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Ile,seq:cat)
     gene            complement(493..1314)
                     /locus_tag="ACHU0J_004123"
     CDS             complement(493..1314)
                     /locus_tag="ACHU0J_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1218544.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(1329..1685)
                     /locus_tag="ACHU0J_004124"
     CDS             complement(1329..1685)
                     /locus_tag="ACHU0J_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1218545.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(1698..>2048)
                     /locus_tag="ACHU0J_004125"
     CDS             complement(1698..>2048)
                     /locus_tag="ACHU0J_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1218546.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          505 a          577 c          437 g          529 t
ORIGIN      
        1 tatttttgac aaatatataa tattttcaac aatatcctga gccaggtata tttcagtata
       61 aggctctgcc ggaaggaatc tggaagaatg aatatggcgc gctgtactgg attcgaacca
      121 gtgaccgatt gcttagaagg caattgctct gtccggctga gctaacaacg ctgaataccg
      181 ataatggacc gccatcgggg acccgccccc cgcaccaaca accctgttat cgtgtcgtct
      241 gctcttcctg ataagctaat ggcggtttgt gatggtggcc cttgctggat ttgaaccagc
      301 gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg gagcagaata
      361 ataacggtcc gtaattaatt tcgcaataaa aaaacccgct cagcggcggg ttgtagaaac
      421 tcttctaacg ccaggcataa aacgcccatc attatgacga atttaccaca gattccggaa
      481 aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg cttcttcaat
      541 atcaaaccgg gtcaccagcg catcataaaa tttcttaact gttttttccc atgacgcgcg
      601 tgttatctgg tttgtcacct cgcatatagc attaaatgcc tccgttgatg gtagtctttc
      661 atagccacga ccaccacaac gctggcagtc tctgataaca ggcataccac gttttaccga
      721 ctcttcacga tgaatggcga caccacgccc acggcaatcc ttacaggcgg tggaaacctc
      781 accctttccg ccacactccg gacaggcaac ttttaccacc tccctgactt ttttccattc
      841 ttcccagtaa aacggataca cacctttcgt acactttgcc cataccggcg gcttaccatc
      901 cggatactgg atcttgtttg taaaaactat gctttcaata aatttttccc catagcaaca
      961 agggcactgc tttttactcg ctgcgctgcg ggcataatcc tcaaaagcgt acgaagccat
     1021 aatgcgcatc actaccggtt ttatttctgc cggaagtttt ctcaacgccg ccacacgatc
     1081 gcaccgactg agtgcataat ctgccagtaa ttctgttgcc cgcgccctgt cattcatact
     1141 gatgcccatt ttccccagga acgcagaaaa acccatctca gcccgattct gtgtcatgcc
     1201 ctgcgcggcc atcacatcag tgatactcag cgcatctttt gacgttgagg ccgatgcatc
     1261 ggtcaggcca ggggattttg gggagtagta tttcggtaaa tcttccagtt tcattttttg
     1321 acctgctctt aatgcattat ggggtaaatc ttcaccccca gacgtccacc agatactggc
     1381 tgaccacgaa cgatattgat ttcatcaaac tgctcatcgt ccattaacac tcccgcatgc
     1441 gtcagcgcat ccagcggtgc tttcaggata ttgtccaggt cgcgacgacg cttatccggt
     1501 ggctctgcaa tcacctttat cgccagcctt ccggacaggc ttaatttcag ccgctgctgg
     1561 cgaacaataa gcgccacagc ccggcgataa cgctttccct cctccgagat aaaatatgtg
     1621 ctgccacggc gtcgccagta agtgttcacc gtcggcgggt aaggtaaaac caaatctatg
     1681 agcatcagtc acctctttta cccaagcacg ccagttgcaa aggcgtgatc aagaaaacga
     1741 aaaattaaat caacctgaga accatgcttt tcttcgaacg ccagaggatc cgcatgaagc
     1801 tcgttgtgat gctcccgaca cagcggtagc gtgaaaatat cgtgagattt ggtccccatt
     1861 ccgccctgac catgaccaat caggtgatgg ggatcgtcgg ctggcttacc acaacacgca
     1921 cacggctgtg tcttcaccca gcgtttgtat ttctcgttaa cccagcggcg acgtttaggt
     1981 cgtttcatga aagattccgg agactcagga tcaacggcaa tgctgaccac cgtcttttcc
     2041 tgttgtgg
//
LOCUS       DAZPDE010000326         2047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPDE010000326 DAZPDE010000000
VERSION     DAZPDE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.333"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..348
                     /gene="spa15"
                     /locus_tag="ACHU0J_004126"
                     /gene_synonym="spaK"
     CDS             <1..348
                     /gene="spa15"
                     /locus_tag="ACHU0J_004126"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR1218547.1"
                     /translation="CPPSIITDLDSHSAITISLDSMPAINIALVNEQVMLWANFDAPS
                     DVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRVVIKDDYVHDGIVFAEILH
                     EFYQRMEILNGVL"
     gene            352..1644
                     /gene="spaL"
                     /locus_tag="ACHU0J_004127"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             352..1644
                     /gene="spaL"
                     /locus_tag="ACHU0J_004127"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR1218548.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1737..>2047
                     /gene="spa13"
                     /locus_tag="ACHU0J_004128"
                     /gene_synonym="spaM"
     CDS             1737..>2047
                     /gene="spa13"
                     /locus_tag="ACHU0J_004128"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR1218549.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     S"
BASE COUNT          648 a          311 c          444 g          644 t
ORIGIN      
        1 tgtcctccat caattattac agatttagac agccattcag cgattaccat ttctttggat
       61 agtatgcctg ctattaatat agctcttgtc aatgagcagg ttatgttatg ggctaatttt
      121 gatgcaccaa gtgatgtaaa acttcagtca tctgcttata acatattaaa tttaatgtta
      181 atgaacttta gttatagcat taatgagctg gtagagcttc atcgttctga tgagtactta
      241 cagttaagag ttgttattaa agatgactac gttcacgatg ggattgtttt tgcagagatt
      301 ctgcatgagt tttatcagag gatggaaatc ttaaatgggg tcttataatc aatgagctat
      361 acaaaattgc tcactcaatt atcttttcct aatagaatct cggggccaat cttggaaaca
      421 agtcttagcg atgtttcgat tggtgagatt tgtaacattc aggctggaat tgaaagtaat
      481 gaaattgttg caagagctca ggttgtagga tttcatgatg aaaaaacaat attaagcttg
      541 attggaaatt ctcgtggact ttcacggcaa acgttgatta agcccactgc ccagtttctt
      601 catacgcaag tcggccgtgg attattggga gcagtagtca atcctttagg ggaggttact
      661 gataagtttg ctgttacaga taacagtgaa attctttatc gacctgtaga taatgctcct
      721 ccgctatata gtgaaagggc tgcaattgag aagccttttt taacaggtat taaggttatt
      781 gattctttac tcacgtgtgg tgaaggacag cgaatgggga tttttgcgtc agctggttgt
      841 ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg gtgctgatat atatgttatt
      901 gggttaattg gtgagcgagg tcgagaggtt actgaaacgg ttgattattt gaaaaactct
      961 gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg attactcttc ggttgatcgt
     1021 tgtaatgctg catatatagc cactgctata gccgaatttt ttaggactga aggacataaa
     1081 gtagcgcttt ttattgattc attaacaagg tatgccagag cattacgtga tgtggcctta
     1141 gccgctggag aatcacctgc cagaagaggc tatccggttt cggtttttga tagcttaccc
     1201 agacttcttg aaaggccagg aaagttaaag gcaggtggct ctattactgc attttacact
     1261 gttcttttgg aggatgatga ttttgctgat ccattagctg aagaggtaag atccatttta
     1321 gatggacata tatatttgag cagaaatcta gcccaaaaag gacaatttcc tgcaattgat
     1381 tccttaaaaa gtataagcag ggtatttaca caggttgttg atgaaaaaca tcgtattatg
     1441 gccgctgcat ttcgggagct actttctgaa atagaagagc taaggacaat tattgacttt
     1501 ggtgaataca aaccggggga gaatgcctct caggataaaa tctacaacaa aatatctgtt
     1561 gttgagagtt ttctgaagca agattatcga ctcggtttta cttatgagca gacaatggag
     1621 cttattggtg aaacaattag ataaggtttt aaaaatcaaa gataaatatc aacggtctgt
     1681 taaattaatt gaagcacaca tattaacact aaaaaaaaaa ctctttgtat cgagatgtgg
     1741 aggcattaga taaaaggatt atatacttcc tgcaactgga gaatgactta gaacctgtag
     1801 gcgctcagag tgttagtcag cttttcaata caagacgtaa gatcgctata gtaaaaaagc
     1861 atattatcca atatcagtct gaacgaattc ttctaaaggg aagaattgaa gaaattcaaa
     1921 aagatattga tgaggcaaat gcttctaaaa gaaaactgct tcataaagag agtaaaatat
     1981 gtaaacggat tggcttgatt aaaagaaata actttgccaa acagttgata cttgatgagt
     2041 tatcaca
//
LOCUS       DAZPDE010000327         2046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPDE010000327 DAZPDE010000000
VERSION     DAZPDE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.334"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(57..326)
                     /locus_tag="ACHU0J_004129"
     CDS             complement(57..326)
                     /locus_tag="ACHU0J_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1218550.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            383..1051
                     /locus_tag="ACHU0J_004130"
     CDS             383..1051
                     /locus_tag="ACHU0J_004130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1218551.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1367..>2046
                     /locus_tag="ACHU0J_004131"
     CDS             1367..>2046
                     /locus_tag="ACHU0J_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218552.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          381 g          602 t
ORIGIN      
        1 accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac
       61 attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      121 agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc
      181 tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac
      241 tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt
      301 tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt
      361 aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc
      421 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
      481 tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt
      541 aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc acgtacctga
      601 gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag
      661 ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcctgc
      721 ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg
      781 aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgatgatgg
      841 aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa
      901 actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg
      961 cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt
     1021 agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag
     1081 acgtgctacg gtttccttta cgatttgggg ttgcgacttt acctctattg ataatgcatt
     1141 ccggcagaac gttcaaatat gaacgtacga tatttaacta accgaaaaac aaaatatagc
     1201 acaggcgaga catttaccat ctaagaaaat agtatcgttt ttttacagtt aaatcaacat
     1261 cacttcctta aaatgaaaac aataaatttc aatcctgaaa cattttattt cagcttatta
     1321 ttccccagta tcacatttta tcaggatatc cttctgcgag gttattatgc ttcctgtaaa
     1381 taatcccccc ctatccactg gaaacgtctc tttttacaga actacatcaa tcgacaatgt
     1441 tcacaataat tatctctccg aatgggttga atggactaaa aacagcattt ccggagaaaa
     1501 cagggaaact gcttttaccc ggctccaatt atgtctggag aacagtgaaa catcgttgga
     1561 cttatcttgt ttaggtctca gatctctacc acgattgcct gacaatcttg atgaaattaa
     1621 tgtaagcaat aaccaactat caatgctccc cgagctacca agggcattga aagagctgaa
     1681 tgcaagcagt aatcaattat ctgcacttcc tgaattacca gtgtcgctgg aatatataaa
     1741 tgtgagtgat aaccatttgt tcgcacttcc tgaattacct gcgtcactag aatatattaa
     1801 tgtaagtgac aatcacctgt ctgtacttcc gaggttacca atgtcattgg aattacttga
     1861 tgcagccaga aatgctttgg aagtaatacc agattttcca gaaagagatg atcatattat
     1921 aagaatattc tggcttaatc agaaccggat cacggcaatt ccggaaagca tacttggcct
     1981 cagttctgat agcgttgtca atcttagaga aaatcaacta tctcccagaa taatgcaaac
     2041 tttgtt
//
LOCUS       DAZPDE010000328         2042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPDE010000328 DAZPDE010000000
VERSION     DAZPDE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.335"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            148..1230
                     /locus_tag="ACHU0J_004132"
     CDS             148..1230
                     /locus_tag="ACHU0J_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1218553.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1719..2042)
                     /locus_tag="ACHU0J_004133"
                     /pseudo
     CDS             complement(1719..2042)
                     /locus_tag="ACHU0J_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          600 a          424 c          409 g          609 t
ORIGIN      
        1 gaaacagttc agataaaaat agggatctac ttcacaattt aaatgagaaa ctaaaactta
       61 catcttgaaa taatctcatt gattggatga atatttgtag tgcagtgaca tcatttttaa
      121 ataatagttc aaataaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta
      181 gctgcatcag tactgatggc gatgtctgct caggcagcag agatttacaa taaagacagt
      241 aacaagctgg atctgtacgg aaaagttaat gctaagcact acttctcttc taacgatgca
      301 gatgatggcg acacaactta tgttcgtctg ggcttcaaag gcgaaactca aatcaacgat
      361 caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa
      421 ggttcttcca aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc
      481 tccatcgatt atggccgtaa ctacggtgta gcatacgata tcggtgcatg gaccgacgtt
      541 ctgccagaat tcggtggcga tacctggacc caaacagatg tgttcatgac tggtcgcacc
      601 acaggtgttg caacctatcg taacaacgac ttctttggtc tggttgatgg tctgaacttt
      661 gctgctcagt atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa cggtgatggt
      721 ttcggtttct ccactactta tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa
      781 tctgaccgca ctaatgatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa
      841 aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctacc
      901 acctattctg aaacccagaa catgactgtt tttggtaata accatattgc caacaaagca
      961 caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct
     1021 tacctgcaat ctaaaggaaa agacttgggt gcgtggggtg atcaggacct gattgaatat
     1081 attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgattacaaa
     1141 atcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc
     1201 gttgctgtag gattggttta ccagttctaa tctgattact caaagatatg ttgtgggagg
     1261 ctttgcctcc ccaacatata agtggcgctc tcaagccact ttccttagaa gcactatctt
     1321 gcttctgact atataaatta ccctttattt tctgggacgt ttcaacgccc catttttaat
     1381 aatactcagt gaacaattaa cgtatcaatt agatttaata acaacgatat ccgtacctaa
     1441 cctgatatct aatgtcataa gcatcccttt aattatcccc tcaacctctt gtaaatttcc
     1501 cactcttgag ctccacatag cccaaacgcc ggatgcaaaa aaaatcatac gtgcatctct
     1561 gaaattgtga tggaggaagt gagtatcaat tatatgtgct tataaacgtt cccgattggc
     1621 acagaagtca ccggattgtt caggctccaa taggttgagt atgtcgtatt tattttccaa
     1681 aataaaaatc gatgctgttg ctccttaata aaatactttt actctctgga cgcttttctt
     1741 agctctcttg gtgttatatt aaaacgatta tggaaccttt cagtaaaacg agaaggacac
     1801 ttgtaaccat tttccctggc aatctcgctg atgggtttta ctgtcgtttg tatagcagga
     1861 caacgcatta tttaacctca cgtcgtccaa tatactttga aaacttattc cctcgcttgc
     1921 cagacggcga tgcaatgtgg aaacagaaat gtagagatat ctcgctacct tgtttgctgt
     1981 ccattttgtt ctaggttcgg atagcattag gttataacaa cgacttatta atgattgttt
     2041 ac
//
LOCUS       DAZPDE010000329         2041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPDE010000329 DAZPDE010000000
VERSION     DAZPDE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.336"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(13..901)
                     /locus_tag="ACHU0J_004134"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHU0J_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          476 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
     2041 c
//
LOCUS       DAZPDE010000330         2037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPDE010000330 DAZPDE010000000
VERSION     DAZPDE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.337"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(133..352)
                     /gene="ymgF"
                     /locus_tag="ACHU0J_004135"
                     /pseudo
     CDS             complement(133..352)
                     /gene="ymgF"
                     /locus_tag="ACHU0J_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(484..2007)
                     /locus_tag="ACHU0J_004136"
     CDS             complement(484..2007)
                     /locus_tag="ACHU0J_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1218554.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          609 a          453 c          435 g          540 t
ORIGIN      
        1 tgatatataa aaacaactgt gataacacca aggttgacaa ctatttgcgc aataattcct
       61 ttccttcaat gatccaggcg ttcactgcca gcaacccatt cagccgcata ctgacagtga
      121 gaaactgaaa gattattgct gatccctgtg gctaacgagt ctttctctta aatcgattaa
      181 ccagattgat cgccagccaa acaagggcat taagcaaatt tatcgtcaca agcacttctc
      241 ccagcccggt actgggtttt gcaataagga tgaccaggag ggtgatcagc ataaatgcaa
      301 tggaaaagat gatatagaga gtgaaataat ccagattatt actattattc atgctactaa
      361 tggcaatcct gtcaaaactt tcaaacaatt atagcaaaca aaataatgaa caatagtaac
      421 aagaggcaac gagctaatta gctgtctata attacaccgc actatggcct tactgatcaa
      481 ttttcactca accacaacct tcaccttcgg tttagaaagg ataattttga ccaggtcgat
      541 gtatgtaaca ggtttataga aataataacc ctgctgaaat gtgatattat tttggttcag
      601 atagccaagt tgttctttcg tttcgacacc ttcagcgacg atactgatcg aaagtttacg
      661 cgccagatcc aatacacaat ccagaattcg ggttgattct gggtctgcgt ttacgcggcc
      721 aacgaaactg tgatcaattt tgatataatc aatatgcagg tcatgaagat aagaaagccc
      781 tgagtagcca gtaccgaaat catccagcgc gatgacaaaa ccattttcat gcagaatgtt
      841 caaccgctga accagacttt catcaacatt caatggctca cgctcggtga cttccagaac
      901 aaggtttaaa tcgcggcggg taaaactgtc acggaaattt aaacactcgt cgacaaacgt
      961 cggcgaaata atatgcgaag cgctaaaatt aattccaata tgaaaacctt ccggcagttt
     1021 actcgcgata gcgttcatct gtctggcaac ctgattcatc aggctttgtg taagcggaac
     1081 gattaatccg gatttttcag caagtggaat aaatgccgcg ggtgaaatat atccaccgtg
     1141 aggttgtttc cagcgggcta acacctcaac tccccgcaat gtcccttccc gaccatttac
     1201 cacaggttgg taaaaaggga cgatctcccc attatttatt gcccgtcgca ggatctcttc
     1261 aggcgttgcg cttttattga aatatctatc aagcaggaag gcagcggcgc tggcgattaa
     1321 cagaataaat attaaaatcc caaagccttt atcgataagt ctgtagaagc taaagagtgg
     1381 cggcgggtta aagataatag tgaaatgata ttttgtggag tgaaccttct cggtataggg
     1441 ataatgtcca ttaagcgtca tcacatcagc cgttggtcca ataatgtcat ccgccacgcg
     1501 taatacatac cttacccctt tcaagggtac attaagcgcc ccgcgaatat gttgatcact
     1561 tatcgtaacc aaaatgcgcg tatctgcaaa ttggctctga tagagcagga taggtaatct
     1621 attaacggtg tcgattgccg gagccaacag taaattactg tccgggacaa caggaatacg
     1681 agacagcagg acccgattcc caggcaggga tgtacaccag actattccct gtttgagaat
     1741 gatgattgtg cgcaggtgag gtttcagagc tgcttcagtg ccaagctgat attgctcctc
     1801 gaggtcgcat tccttcccgg caatgttaac cgctgtttga gtcgcctgtg acgcttcttc
     1861 aagtatatga ttgatattgt tggcggcata tcttgctccc gccaggtact ctgcttttgc
     1921 ggaataccag agctggatgt tcaaaatagc gacaccggta ataaacaagc atatcgcaac
     1981 gaggatgggt atgagtgtat tgcgcatttg cttgtcccgg ccaaagggtt taataca
//
LOCUS       DAZPDE010000331         2029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPDE010000331 DAZPDE010000000
VERSION     DAZPDE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.338"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHU0J_004137"
     CDS             complement(<1..1824)
                     /locus_tag="ACHU0J_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR1218555.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHU0J_004138"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHU0J_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218556.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZPDE010000332         2016 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPDE010000332 DAZPDE010000000
VERSION     DAZPDE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.339"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(252..326)
                     /locus_tag="ACHU0J_004139"
     tRNA            complement(252..326)
                     /locus_tag="ACHU0J_004139"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Gly,seq:tcc)
     gene            complement(330..405)
                     /locus_tag="ACHU0J_004140"
     tRNA            complement(330..405)
                     /locus_tag="ACHU0J_004140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:tgt)
     gene            complement(408..484)
                     /locus_tag="ACHU0J_004141"
     tRNA            complement(408..484)
                     /locus_tag="ACHU0J_004141"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(448..450),aa:Arg,seq:tct)
     gene            complement(486..561)
                     /locus_tag="ACHU0J_004142"
     tRNA            complement(486..561)
                     /locus_tag="ACHU0J_004142"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:Ile,seq:cat)
     gene            complement(738..1427)
                     /locus_tag="ACHU0J_004143"
     CDS             complement(738..1427)
                     /locus_tag="ACHU0J_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1218557.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1424..1783)
                     /locus_tag="ACHU0J_004144"
     CDS             complement(1424..1783)
                     /locus_tag="ACHU0J_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1218558.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1783..1971)
                     /locus_tag="ACHU0J_004145"
     CDS             complement(1783..1971)
                     /locus_tag="ACHU0J_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218559.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          515 a          550 c          433 g          518 t
ORIGIN      
        1 tgttcccggg gtttgcttag aatattttgt aagttgtccg aagagatatt tacaacacca
       61 gaatgatgaa tcatcagtcc cctgccagag gttcactacg taacctgacc gacaatgcac
      121 cattctggtg ctgaaaaaag agcactggaa ctgcaacaaa acaacatcac agaacaggag
      181 atggtaagga gtcagacatt cccacacaat attgtgtcag tgcaaataac aaccttcgtc
      241 tcaaatctcg ctggagcggg cagcgggaat cgaacccgca tcatcagctt ggaaggctga
      301 ggtaatagcc attatacgat gcccgcatat ggtgccgact accggaatcg aactggtgac
      361 ctactgatta caagtcagtt gctctgcctg ctgagctaag tcggcgctgg cccaccaccg
      421 aggactcgaa cctcggaccg tcaacttaga aggttgatgc tctatccgga tgagctagtg
      481 gtggttggtg gcccttgctg gacttgaacc agcgacctgg cgattatgag tcgctcgctc
      541 tcaccactga gctaaagggc cggaggcaga ataataacca tatgtcatca catctgcaaa
      601 ctcatctgac caccagcacg tttaacgtcc tgtgccattt ttcaggcata aaaaaaccgc
      661 tcggcggcgg gttttgctta ctttaccatc gcgtacaaaa tcggcaaagt atcagatttg
      721 catgagatat atgcctttta atctactttt gcaatacttt gctttgaaaa tgccgctttt
      781 tgttctgaac gtgttctcat cacaaacaat aaagcctcac tatccagtcg gtgaaaaatg
      841 tgtttcatta caacccagtg accagtaaat gttttggacc agtttttggt tgttactccc
      901 gccagtaatg ccagctcctg atattcataa ccttccccac caaaaagttc tgcttttacc
      961 gcctgcgccg ccagccagat taattttttc aggcgttcct gtgttttctc tgcaattttc
     1021 ctagtgccag aatgagcctt aaactcttcc cacgcccagt gggttatggt gatctgatac
     1081 tcccagcaaa tacttccgct gtaacaccac aacaaccagg ctttatagtg ctcttcaaga
     1141 gacagaacag cccgccgcca cgatgatgtc gaaaacgcaa cctgactgac cagaggaatt
     1201 gacgccccct tcgccagtga ttgttttccc gggattggtg gattatcccg cgttatcatt
     1261 tttccggtca cctcatcgcg gtaccggatt tttttacgcc tgtaacgccc tgtatcgaac
     1321 atggcattct cctgccaggc ttcaagctga ccttttgtcg ccccactcaa atcagcggtg
     1381 gcgataatga gctgctcacg aacaaactgt aaatactggt tattcatgca cactccagct
     1441 ctgtgatttt tatccccaac cgaccaccag gaacaggcag tccgcgcaca atattggttt
     1501 catcaaactg ctcgtcgtcg ataagcaacc ccgcatgtgt cagtgcatcc agtggtgctt
     1561 tcaggatatt gtccaggtca cgacgccgct tatccggtgg ctctgcaata atctttatcg
     1621 ccagtcttcc ggacaggctt aatttcagtc gctgctgacg gacaataagc gccacatccc
     1681 ggcgataacg ctcaccggct tttgatacaa aatatgtgct gccacgacgt cgccagtaag
     1741 tattcaccgt cggcggataa ggtaaaataa actcatggcg catcagcgca gcacctcctg
     1801 caccagcttt tcaaactttc cgactctggt ttccagctct gccacacagt ccaccagctc
     1861 atctactgct ttctgtgcgc gatgcttcgc ctgcatcagt tcccgaagcg cgggtaccat
     1921 attgaggtag cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag
     1981 ccaataatga gcagaaaaac ccaacgttac tctaaa
//
LOCUS       DAZPDE010000333         2003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPDE010000333 DAZPDE010000000
VERSION     DAZPDE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.340"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHU0J_004146"
     CDS             <1..360
                     /locus_tag="ACHU0J_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1218560.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHU0J_004147"
     CDS             361..726
                     /locus_tag="ACHU0J_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1218561.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHU0J_004148"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHU0J_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHU0J_004149"
     tRNA            1605..1680
                     /locus_tag="ACHU0J_004149"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHU0J_004150"
     tRNA            1682..1758
                     /locus_tag="ACHU0J_004150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHU0J_004151"
     tRNA            1761..1836
                     /locus_tag="ACHU0J_004151"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHU0J_004152"
     tRNA            1840..1914
                     /locus_tag="ACHU0J_004152"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
BASE COUNT          543 a          427 c          547 g          486 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa att
//
LOCUS       DAZPDE010000334         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPDE010000334 DAZPDE010000000
VERSION     DAZPDE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.341"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHU0J_004153"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHU0J_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHU0J_004154"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHU0J_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHU0J_004155"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHU0J_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR1218562.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHU0J_004156"
     CDS             1708..>1969
                     /locus_tag="ACHU0J_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1218563.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTACGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          433 c          462 g          514 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZPDE010000335         1958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPDE010000335 DAZPDE010000000
VERSION     DAZPDE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.342"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            327..521
                     /locus_tag="ACHU0J_004157"
     CDS             327..521
                     /locus_tag="ACHU0J_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218564.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            529..1941
                     /gene="pykF"
                     /locus_tag="ACHU0J_004158"
     CDS             529..1941
                     /gene="pykF"
                     /locus_tag="ACHU0J_004158"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1218565.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          549 a          478 c          443 g          488 t
ORIGIN      
        1 tcagcatata tcaatctaac caattaaaca aaaatcaaac aaaatcagac aaataacgcg
       61 ataaattatt ttaactgtag caattgagcg atgatatatt tatacaccgg atgaactttc
      121 acttatcctc acactgacaa cttcggcacc agacgttgcg caaacagcga agtttttgcg
      181 taaccttttc cctggaacgt taaatctttg ataacaattt attgtctaac aagttgtata
      241 ttttttgaaa cgctgttttt gttttccttt tggattaatt tcagcgtata atgcgcgcca
      301 attgactctt gaatggtttc agcactttgg actgtagaac tcaacgactc aaaaacagac
      361 actcacgttg ggctgagaca caagcacaca ttcctctgca cgctttttcg atgtcaccta
      421 tccttagagc gaggcaccac cactttcgta ataccggatt cgctttccgg cagtgcgccc
      481 agaaagcaag tttctcccat ccttctcaac ttaaagacta agactgtcat gaaaaagacc
      541 aaaattgttt gcaccatcgg accgaaaacc gaatctgaag agatgttagc taaaatgctg
      601 gacgctggca tgaacgttat gcgtctgaac ttctctcatg gtgactatgc agaacacggt
      661 cagcgcattc agaatctgcg caacgtgatg agcaaaactg gtaaaaccgc cgctatcctg
      721 cttgatacca aaggtccgga aatccgcacc atgaaactgg aaggcggtaa cgatgtttct
      781 ctgaaagctg gtcagacctt tactttcacc actgataaat ctgttatcgg caacagcgaa
      841 atggttgcgg taacgtatga aggtttcact actgacctgt ctgttggcaa caccgtactg
      901 gttgacgatg gtctgatcgg tatggaagtt accgccattg aaggtaacaa agttatctgt
      961 aaagtgctga acaacggtga cctgggcgaa aacaaaggtg tgaacctgcc tggcgtttcc
     1021 attgctctgc cagcactggc tgaaaaagac aaacaggacc tgatctttgg ttgcgaaaaa
     1081 ggcgtagact ttgttgctgc ttcctttatt cgtaagcgtt ctgacgttat cgaaatccgt
     1141 gagcacctga aagcgcacgg cggcgaaaac atccacatca tctccaaaat cgaaaaccag
     1201 gaaggcctca acaacttcga cgaaatcctc gaagcctctg acggcatcat ggttgcgcgt
     1261 ggcgacctgg gtgtagaaat cccggtagaa gaagttatct tcgcccagaa gatgatgatc
     1321 gaaaaatgta tccgtgcacg taaagtcgtt atcactgcga cccagatgct ggattccatg
     1381 atcaaaaacc cacgcccgac tcgcgcagaa gccggtgacg ttgcaaacgc catcctcgac
     1441 ggtactgacg cagtgatgct gtctggtgaa tccgcaaaag gtaaataccc gctggaagcg
     1501 gtttctatca tggcgaccat ctgcgaacgt accgaccgcg tgatgaacag ccgtctcgag
     1561 ttcaacaatg acaaccgtaa actgcgcatc accgaagcgg tatgccgtgg tgccgttgaa
     1621 actgctgaaa aactggatgc tccgctgatc gtggttgcta cccagggcgg taaatctgct
     1681 cgcgcagtac gtaaatactt cccggatgcc accatcctgg cactgaccac caacgaaaaa
     1741 acggctcatc agttggtact gagcaaaggc gttgtgccgc agctggttaa agagatcact
     1801 tctactgatg atttctaccg tctgggtaaa gaactggctc tgcagagcgg tctggcacac
     1861 aaaggtgacg tcgtagttat ggtttctggt gcactggtac cgagcggcac tactaacacc
     1921 gcatctgttc acgtcctgta atattgcttt tgtgaatt
//
LOCUS       DAZPDE010000336         1935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPDE010000336 DAZPDE010000000
VERSION     DAZPDE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.343"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHU0J_004159"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHU0J_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1218566.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHU0J_004160"
     CDS             complement(590..1255)
                     /locus_tag="ACHU0J_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1218567.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1424..1780)
                     /locus_tag="ACHU0J_004161"
     CDS             complement(1424..1780)
                     /locus_tag="ACHU0J_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218568.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1838..>1935)
                     /locus_tag="ACHU0J_004162"
     CDS             complement(1838..>1935)
                     /locus_tag="ACHU0J_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1218569.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          466 a          502 c          430 g          537 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctccccg ccctttcggg cggtctcctg atgatttgag ggtgcagaaa
     1381 tccctccggt taaggattaa atttttaaca gtgctaaatt taattattca gttctggatt
     1441 ttgtcgccct gcgtatccgc gctttcgcgt tacgctcaat ctgtatcagc ttttctatat
     1501 ttctccgcct ttcctgttcc tcctggcgca atagccttac atcatctgcc agtctggttt
     1561 ctctttttgc cacagagagc atccagtcaa acggctccac aaccgcaccg cagattttac
     1621 agcggacctg acgctctttt tcgtcaaccc gtacagaggc gtgatggcaa tatggtcttt
     1681 ccgatggctt ataaagaaaa ttaacctgat tacgagggtc atcctctttt atcggaaata
     1741 aaacgatatt gcttaactca tcttctggtt ttatttccat gctcctctcc tttgatgcga
     1801 atgccagcgg taattgaagc ctgatagcta atttcattca cagtatcgcc tcctgaaaat
     1861 taccctgata gaaagccagt acacgctgca tagcttcgct cttccggcac tcgcgacaga
     1921 ttatattcag gcgcc
//
LOCUS       DAZPDE010000337         1919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPDE010000337 DAZPDE010000000
VERSION     DAZPDE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.344"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..63)
                     /locus_tag="ACHU0J_004163"
     CDS             complement(<1..63)
                     /locus_tag="ACHU0J_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218570.1"
                     /translation="MYNNKIDSNRKIRHDWVDALK"
     gene            514..1704
                     /locus_tag="ACHU0J_004164"
     CDS             514..1704
                     /locus_tag="ACHU0J_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR1218571.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
BASE COUNT          525 a          402 c          432 g          560 t
ORIGIN      
        1 ttttaaagca tcaacccaat catgtcttat ttttctgttg ctgtcgattt tgttattata
       61 catttaaagt aggctctcca gttttgtgtg atttttaaac actcattgca aatatgaatg
      121 cctgaaagtt tatgattatt aatcaacgag ttcaatatta atttattatt tttcaaagtc
      181 gcactatata ggcatttact tccgcagtac caccgatagt atcgatatca aatcagcctg
      241 cggccgcgat aactttcgta acattctgcc agcggctttg ttgaataaat caggttcaag
      301 ataatcgtct ctgcatcagg ttagagtttc aggcaataca tcctctttct ggcatacctg
      361 ccactctcgc accatggtca tagcctttac gatctgcata ctcaccagac cagagcccgc
      421 tcccttttag gctgaggaat ccccagaaaa gaagacaggc atagagtttt gtgatctact
      481 gattgcgcta acaaattcaa tgagatcacc tctatgcctg cccgtaaagt atgccagact
      541 tttttccgta atgctttagc ttccacgcat cagtatcgac aaaatgccat tattgattct
      601 gctgctgctt tagtcggtgg cgcatctctt tctcttacca gcattggccg gcatttaccc
      661 ggtcctgctc gcgtgaaaga taaaataaaa cgcgttgact gtctgttggg taataaacga
      721 cttcataatg atatccctct gatattcaaa aatattacat ccatgatgac aaataaactg
      781 tcgtggtgtg ttatcgctgt tgactggagc ggttaccctt ttcaggaata tcatgtcctt
      841 cgtgccagcc tcctctgcga cggtcgctct atccccctga tgagtcaggt cttcccgtca
      901 aaaaaaaata acgaggcggt ggaaatcgct tttctggatg cgctttccgg ggccatttct
      961 ccccggactc gtgttgttat tgttactgat gctggttttc aaagtgcatg gttccgtcat
     1021 atcaaatcac agggatggga ttttattggt cgtatcaggg gggtagtgaa gttcaggctg
     1081 gatagtgaca aagataagtg gctggacata aaaatgtgca gggggagttc agaggcaaaa
     1141 tacctgggga cgggaaccct ggcccggaaa aaacgctcgc agtgtgaagg acatttttat
     1201 ctttataaac attcacctaa aggcagaaaa agcaggcgtg ccagagggag gccgggctta
     1261 ccaacaacgg aaaaagaaca aaaagccgca ggcagagaac catggttaat attcagcaat
     1321 acagctgagt ttaatgccaa aaaaatcatg aaactgtaca gtcgaagaat gcagatagag
     1381 caaaactttc gggatgaaaa aagtgaacgc tttggttttg gccttcgggc gagcagaagt
     1441 cggaggggag agcgttttct ggtattaagt ctactggtta cactggccag tatcgtactc
     1501 tggctacttg gttatcattt tgaaaataaa ggatttcatt taaagtatca ggctaatagt
     1561 ctcaaaaaac gaagagtatt gtcttttctg acgctggctg aaaacgtcct gagatttaac
     1621 cctgaacttc tacgacgagc acaacctgaa aagatattgt gccggttggc cagcacttac
     1681 cgaagtatgg tgctggctta ctgatgaaaa ttttctgggg atccctcagc ctttcgggga
     1741 ggaatgaggg ctctgatatt cttttgccgt agttcatcgt gaaaccgtcg gatgtcattt
     1801 ggcctgagtg acccatgcct cccaaaagca gagtcgctag tgttatgtaa ttaggcggtt
     1861 aaacttcaat tctcagtgtt aagtttgggt aaacgcttgt aaatggtatt gctaatttt
//
LOCUS       DAZPDE010000338         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPDE010000338 DAZPDE010000000
VERSION     DAZPDE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.345"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(854..1252)
                     /locus_tag="ACHU0J_004165"
                     /pseudo
     CDS             complement(854..1252)
                     /locus_tag="ACHU0J_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1255..1621)
                     /locus_tag="ACHU0J_004166"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="ACHU0J_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1913)
                     /locus_tag="ACHU0J_004167"
                     /pseudo
     CDS             complement(1623..1913)
                     /locus_tag="ACHU0J_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          536 c          459 g          493 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgcggacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tga
//
LOCUS       DAZPDE010000339         1887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPDE010000339 DAZPDE010000000
VERSION     DAZPDE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.346"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHU0J_004168"
     CDS             <1..197
                     /locus_tag="ACHU0J_004168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218572.1"
                     /translation="GSFFVHCGFLHANMIYFQLNQLDMILPLISLMKVWGGGNAISVE
                     NSVFVMTGSFTAFLMPENVH"
     gene            507..911
                     /gene="stpA"
                     /locus_tag="ACHU0J_004169"
     CDS             507..911
                     /gene="stpA"
                     /locus_tag="ACHU0J_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1218573.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(958..1482)
                     /gene="ygaP"
                     /locus_tag="ACHU0J_004170"
     CDS             complement(958..1482)
                     /gene="ygaP"
                     /locus_tag="ACHU0J_004170"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1218574.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1492..1791)
                     /locus_tag="ACHU0J_004171"
     CDS             complement(1492..1791)
                     /locus_tag="ACHU0J_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1218575.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          525 a          406 c          430 g          526 t
ORIGIN      
        1 acggctcgtt ttttgtacat tgtggatttt tacatgccaa tatgatttat tttcaattaa
       61 atcaattaga tatgatcttg cctttaattt cactaatgaa agtttggggg ggaggtaacg
      121 ctataagcgt agagaattcg gtgtttgtca tgaccgggtc atttacagca tttttgatgc
      181 cagaaaatgt acattaacag cagaaagcac cagtgagggg tgttttcttt tattctgtta
      241 tattgcattt ttattcactt cggttttgtc aggcttgcgg agttagcgag cagagagcgc
      301 cactctgttc accaataacc taaattactc atcaaaatgg ctaatatata atcttgaaat
      361 tattctgaga gtttcagaaa ataacagctg aaataatctc gcgcaggact gtaaatagat
      421 taaattttgt ggaaatataa taagtgatcg cttacactac gcgacgaaat acttttttgt
      481 tttggcgtta aaaggttttc tttattatgt ccgtaatgtt acaaagttta aataacattc
      541 gcaccctccg tgcgatggct cgcgaattct ccattgacgt tcttgaagaa atgctcgaaa
      601 aattcagggt tgtcactaaa gaaagacgtg aagaagaaga acagcagcag cgtgaactgg
      661 ctgagcgtca ggaaaaaatt agcacctggc tggagctgat gaaagctgac ggaattaacc
      721 cggaagagtt attgggtaat agctctgctg ctgcaccacg tgctggtaaa aaacgccagc
      781 cgcgtccggc gaaatataaa ttcactgatg ttaacggtga aactaaaacc tggaccggtc
      841 agggccgtac accgaagcca attgctcagg cgctggcaga aggtaaatct ctcgacgatt
      901 tcctgatcta attactgagg ccggatgtcg ctgctagggc gcgtccggcg tcgcttctca
      961 agctcgttgg ttccacggca tcttatctaa caaccttgcc attccacaaa aaccgctgat
     1021 tcctgcaaac agtaacccgg caccaacaaa gccacttaat aagaagaaac cgctatttac
     1081 ggtataaccc agtacaacgc cgtttaatat taaaccaccc gcagcgatct gcacctggcg
     1141 cattaacggc aagggttgag atttatttac cgctactggc aatccagctc ttttccagcc
     1201 atcaatccca tcttcgagta aaaatatttc tgcgggggcg gcaatcgctg ctaatttatc
     1261 ggcgttatta ctggtgcgtt tacctgcctg gcagtggaat ataatttgct cgcgacgtaa
     1321 tttaggtgga agacctgact gttccagcac ggataatgga gccagatctg cttcaggaat
     1381 atgttcacga agatattcat cagcgtcacg aatatcgatt aactttgcgc cgcgtgcgat
     1441 taattcttgt gcatcatgcg gcgaaatggt tgtcaaagcc atagtgactc cttacggaca
     1501 atagacattt ttcagggtgg cgataatggc atttaccgcc tcatttttaa tggaatatag
     1561 aatgcgttgg gcatcccgtt ggctgttgat aagcccttcg tcccgcatac gagcgagatg
     1621 ctgtgatgtc gcagaggcgc tcagtccggt aatgcgcgtc agctctcccg cgctggtgcc
     1681 gggggaaccg ctaagcatgc acagaatcag caaccgttta gggtggctca ttgctttcaa
     1741 taaggccgct gcctgttcgg tactggcctg taattgcgcg agttcagtca tatttattta
     1801 agtattttct aaattaagta aactctaaac taaaaatgca acatatacca gcctcagcag
     1861 cgtaaatgag agtaaaagcg taagctg
//
LOCUS       DAZPDE010000340         1882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPDE010000340 DAZPDE010000000
VERSION     DAZPDE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.347"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            40..717
                     /gene="ospD1"
                     /locus_tag="ACHU0J_004172"
     CDS             40..717
                     /gene="ospD1"
                     /locus_tag="ACHU0J_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR1218576.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            1041..1151
                     /locus_tag="ACHU0J_004173"
                     /pseudo
     CDS             1041..1151
                     /locus_tag="ACHU0J_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1229..1543
                     /locus_tag="ACHU0J_004174"
     CDS             1229..1543
                     /locus_tag="ACHU0J_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218577.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
BASE COUNT          634 a          356 c          384 g          508 t
ORIGIN      
        1 taggttattg ttttttttta gcaaaaagga tgaacaaaaa tgtcaataaa taactatgga
       61 ttacatccag caaacaacaa aaatatgcac ttaataatag gcagcaatac tgctaatgaa
      121 aataaaggaa tgaaaaataa tatcattaac gtgacaaata ccgctatatc ccacgccatc
      181 aatgaagaaa aatcaggggg gggatatagt ggtgtttctt tcagaaaatt ggccaaaata
      241 cagaacatat ccattccgac aaagaataat aaggagtata accgccataa tttgttttca
      301 ttgatttggc atggaaatgc cgatgcagcg cgtaaatacg gtgaatcgct gttggcagcc
      361 gaaataccca aagaggaaaa actagaagtt cttgcagcac gaaataatgc tggggaatct
      421 gctttgttca tagctcttca agaaggtcat tccgctgcga ttcaagctta tggagatttt
      481 attaaaactt ttgatttatc accaaaagaa acgattaaac tattggatgt aagagataat
      541 gaggggttac caggattatt tctggccgca gggaaaggga atatcgaggc tatgatggca
      601 tatataaata tatgccatca tagtgggata aaacttacag aaatagcaga cagacttaac
      661 aataatgaac aagacatgtt taatattatt tctgacaaaa tacaagagtt gttttaagtg
      721 tgctaaatag ctgcaaagaa ttgcacttag aaggatctct ccccgaattg tgtgatctgc
      781 ttaatttcta aaaagaaaca aacagttaac tagactgagc gatactaccc atagaaccag
      841 tttcccatga cgaacgacac tggacgcaaa aacaatcata aaaaacacaa taaatgtaat
      901 gaacacatcc caccctatta agtaaagtgc actgtgatag aggaagggaa atacaggaag
      961 gtgtggagcc ttgcagattt ctggcatcat agtgcccaat acggaatgca taaagccctc
     1021 tcagactgga gactccacaa atgaaatata cagtgtttaa ttatatcaag tgcgattaca
     1081 atcgctgacg acggcacagt tggtgtggag gtctcagtcc tgaacaattt gaaaaccaga
     1141 acctcactta agcctgtgtt catattacgt gggtaggatc atacttttat agaagctgtc
     1201 agcggctacg tcccagccaa atcacttcat gctccagcgc cagagaggca aggttgggtt
     1261 tgctcagctg ccggttgatt ttgttgcgat tgaaccggac agcgtccagg gcgtcggcaa
     1321 aagagcgaat ctcaccaatc gttgtttcat tatccgcatt aacgatagct tcaagaaacg
     1381 ccaaggtttt atcgaattta tcagcaacag tggctccggg cataccgtga ccgtttatac
     1441 aaaacggcgt tttcagcgtg gagttttcat gaatagcctc aacacgaatg tcgtgaagcc
     1501 agtgatgtat cggctacgat cctcagctga tgccagacca taggactgac actacgaaca
     1561 gcaattttaa tgacgtaaat cttcataact acaactaaca gggcatcatt atgaatgatg
     1621 ccggttattt taccgctgtt ggtcccttca tttagggact gccccaagtt ttttcatact
     1681 ctctttctca gagagtttag tctctgggat tccagacggt ccatcattga gccaagggag
     1741 taaagaactc aaagcattta cagaattgtt tgaatcagct aaaataatga caatgttact
     1801 attttacatg acaaggtgat tataatgctt ggaacatctt ttaataattt tggaatcagt
     1861 ttatctcaca aaagatattt tt
//
LOCUS       DAZPDE010000341         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPDE010000341 DAZPDE010000000
VERSION     DAZPDE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.348"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHU0J_004175"
     CDS             complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHU0J_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR1218578.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="ACHU0J_004176"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="ACHU0J_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR1218579.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="ACHU0J_004177"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="ACHU0J_004177"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR1218580.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="ACHU0J_004178"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="ACHU0J_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1218581.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /gene="rem"
                     /locus_tag="ACHU0J_004179"
     CDS             1250..1501
                     /gene="rem"
                     /locus_tag="ACHU0J_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR1218582.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="ACHU0J_004180"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="ACHU0J_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          409 c          466 g          465 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcggttttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAZPDE010000342         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPDE010000342 DAZPDE010000000
VERSION     DAZPDE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.349"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..355)
                     /locus_tag="ACHU0J_004181"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="ACHU0J_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(355..1275)
                     /locus_tag="ACHU0J_004182"
     CDS             complement(355..1275)
                     /locus_tag="ACHU0J_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1218583.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..>1846
                     /gene="pgrR"
                     /locus_tag="ACHU0J_004183"
     CDS             1413..>1846
                     /gene="pgrR"
                     /locus_tag="ACHU0J_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1218584.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          461 a          453 c          426 g          506 t
ORIGIN      
        1 ggcacccccc acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta
       61 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac
      121 gaggctaccc accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt
      181 tttcagccaa tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
      241 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
      301 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
      361 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
      421 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
      481 ccgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
      541 catcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
      601 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta
      661 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag
      721 tggcataatg gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc
      781 aatgtgtggc agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga
      841 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
      901 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
      961 ataagaaagc gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg
     1021 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
     1081 agtgacaaat cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac
     1141 gcccccacct gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg
     1201 agggaaatag atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
     1261 tttattattc atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata
     1321 tcactataga tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt
     1381 taaatgatta atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga
     1441 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca
     1501 tggtgcagtc agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc
     1561 ttctgacgcg aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg
     1621 ttcttggccc tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga
     1681 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
     1741 taccagccat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg
     1801 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgt
//
LOCUS       DAZPDE010000343         1834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPDE010000343 DAZPDE010000000
VERSION     DAZPDE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.350"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHU0J_004184"
     CDS             complement(<1..182)
                     /locus_tag="ACHU0J_004184"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218585.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(323..877)
                     /locus_tag="ACHU0J_004185"
     CDS             complement(323..877)
                     /locus_tag="ACHU0J_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1218586.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(874..1164)
                     /locus_tag="ACHU0J_004186"
     CDS             complement(874..1164)
                     /locus_tag="ACHU0J_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1218587.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1164..1763)
                     /locus_tag="ACHU0J_004187"
     CDS             complement(1164..1763)
                     /locus_tag="ACHU0J_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1218588.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          422 g          471 t
ORIGIN      
        1 tggttatggg tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact
       61 ccgtacattt ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca
      121 gggttggcgt aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc
      181 ataaatcgcg aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc
      241 gctccgtaaa catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata
      301 tagccccgga taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat
      361 cacgcatcgg tagatagagc atatattcag caagatttag ccatacatct acccgactgc
      421 agcacgtccg gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct
      481 tagtcatccc ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg
      541 ccagcacctc acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca
      601 gccaggtctt ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct
      661 ctattcccgc ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt
      721 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
      781 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      841 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      901 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      961 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg
     1021 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
     1081 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
     1141 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
     1201 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
     1261 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
     1321 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
     1381 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
     1441 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
     1501 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
     1561 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
     1621 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
     1681 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1741 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1801 cccgttatgt agtataccag aattaatgcc aata
//
LOCUS       DAZPDE010000344         1822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPDE010000344 DAZPDE010000000
VERSION     DAZPDE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.351"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHU0J_004188"
     CDS             complement(<1..362)
                     /locus_tag="ACHU0J_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1218589.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHU0J_004189"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHU0J_004189"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR1218590.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          544 a          472 c          376 g          430 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cg
//
LOCUS       DAZPDE010000345         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPDE010000345 DAZPDE010000000
VERSION     DAZPDE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.352"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHU0J_004190"
     CDS             173..1198
                     /locus_tag="ACHU0J_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1218591.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHU0J_004191"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHU0J_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZPDE010000346         1801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPDE010000346 DAZPDE010000000
VERSION     DAZPDE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.353"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1193)
                     /gene="mxiA"
                     /locus_tag="ACHU0J_004192"
                     /gene_synonym="virH"
     CDS             complement(<1..1193)
                     /gene="mxiA"
                     /locus_tag="ACHU0J_004192"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR1218592.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPT"
     gene            complement(1206..>1801)
                     /gene="mxiC"
                     /locus_tag="ACHU0J_004193"
     CDS             complement(1206..>1801)
                     /gene="mxiC"
                     /locus_tag="ACHU0J_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1218593.1"
                     /translation="AGINSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEW
                     LGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMK
                     KLESSSLLKDGSLSAEQLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLS
                     SVNESPHDIFKSESEREIAINILRELVTSAYKKELSR"
BASE COUNT          639 a          372 c          258 g          532 t
ORIGIN      
        1 gttggaagcc tgacaccata atcaataaag aactgacttc ttatcctttc aataagacct
       61 tccatatcat ttgcatttat cttattttcg gcaaataata aaattaaagg aacggtttct
      121 gaactaatag catccagatt ttctatcatt gccaaagatg agtcatggct attatcaata
      181 tcaaatgtac cagtatagcc actagaatca gactcagaca gacttttttc tttttctacg
      241 acctttttat aatagaataa agccgtcaaa gtaactgcta tcaggaaaaa aacaaaaaac
      301 ggaaaacctg gcaacatccc tatcgccaag gcaagagctg atgtaacaat gagaacaaaa
      361 ggattcccaa atatctggga cattatgtta cgtcccatat tatcgctatc accattaact
      421 cttgtaacga taaatcctgc acttatagaa attaacagag caggaatctg agagaccaat
      481 ccgtcaccaa tagttagtat ggtataagta gataatgcac cagataggga cattccatgt
      541 tgactcatac caacagaaat acctcctatt aaattgacaa aaataataat gatgccagca
      601 attgcatcac ctttaatgaa cttcattgct ccatcaaatg aaccatacaa ttgactctca
      661 cgttccagaa tactgcgtct ttctttagct cctgcggcat caataattcc agcctttaaa
      721 tccgcatcaa tgctcatttg tttccccggc ataccgtcaa gagaaaagcg agcagctacc
      781 tccgcaaccc tttcagatcc ttttgttata acaatgaact gcacaacagt tactattgaa
      841 aaaataacaa aacctacagc cagactatca ccaatgacaa actggccaaa tgtagtaata
      901 attttacccg catccgcatc aactaaaatt agtcgactag tacttattga taaagcgaga
      961 cgaaataatg tcgttatcaa caaaaccgaa gggaatgtag aaaaactcaa tattctttca
     1021 atataaaatg atcccataaa aacgagaata gcaagtacaa tattaagccc tattaaaaaa
     1081 tcaacaaggt atgtaggtaa tggtatgatc aacatagcaa tgatcataac cataagcact
     1141 aatataatta attcaggctt agtacttact tgcttaagaa aagactggat cacttttatc
     1201 tcctgttatc tagaaagctc tttcttgtat gcacttgtga caagctctcg tagaatattg
     1261 atcgcaattt ctctttcact ttcactttta aatatatcat gaggactttc attaacagaa
     1321 gatagatatg tttgatacac tacagaatcc tcatgactgg ctcgtgatac ttctataaca
     1381 gaagtaagaa tttgctcact ttcacttgga tattgaaaaa tatataataa agttagcaat
     1441 agctgctctg cacttaaact gccatctttt agcaagcttg aggattccag tttcttcata
     1501 aaaattacgt ctgaagttct aatcatctta attgcaatta atttagataa cacaaaacca
     1561 aactcataag cattacagct cggtttctca gaatccatgt ctacaatcag tgactgctct
     1621 acaaaattca caattgtatg tctgtgatta aaaccaaaat taccaagcca ttctatatac
     1681 tgatctgttg ttgatatgtt ccccatgata aaaccacgat aacatgcacg caaaagctgt
     1741 ggttcaagtt tcattttgtt gccaaataat ttagcctgta tagctgaatt tattccagca
     1801 t
//
LOCUS       DAZPDE010000347         1760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPDE010000347 DAZPDE010000000
VERSION     DAZPDE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.354"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHU0J_004194"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHU0J_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHU0J_004195"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHU0J_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR1218594.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACHU0J_004196"
     CDS             complement(1164..1349)
                     /locus_tag="ACHU0J_004196"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1218595.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..1646)
                     /locus_tag="ACHU0J_004197"
     CDS             complement(1374..1646)
                     /locus_tag="ACHU0J_004197"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR1218596.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAZPDE010000348         1746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPDE010000348 DAZPDE010000000
VERSION     DAZPDE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.355"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            453..1301
                     /locus_tag="ACHU0J_004198"
     CDS             453..1301
                     /locus_tag="ACHU0J_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1218597.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACHU0J_004199"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACHU0J_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1746
                     /locus_tag="ACHU0J_004200"
     CDS             1635..>1746
                     /locus_tag="ACHU0J_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218598.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          498 a          398 c          411 g          439 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagc
//
LOCUS       DAZPDE010000349         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPDE010000349 DAZPDE010000000
VERSION     DAZPDE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.356"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHU0J_004201"
     CDS             complement(<1..353)
                     /locus_tag="ACHU0J_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1218599.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHU0J_004202"
     CDS             complement(350..940)
                     /locus_tag="ACHU0J_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1218600.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHU0J_004203"
     CDS             complement(927..1298)
                     /locus_tag="ACHU0J_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1218601.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHU0J_004204"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHU0J_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1218602.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZPDE010000350         1740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPDE010000350 DAZPDE010000000
VERSION     DAZPDE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.357"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..528)
                     /gene="spa29"
                     /locus_tag="ACHU0J_004205"
                     /gene_synonym="spaR"
     CDS             complement(<1..528)
                     /gene="spa29"
                     /locus_tag="ACHU0J_004205"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR1218603.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLT"
     gene            complement(528..788)
                     /gene="spaQ"
                     /locus_tag="ACHU0J_004206"
     CDS             complement(528..788)
                     /gene="spaQ"
                     /locus_tag="ACHU0J_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR1218604.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(803..1453)
                     /gene="spa24"
                     /locus_tag="ACHU0J_004207"
                     /gene_synonym="spaP"
     CDS             complement(803..1453)
                     /gene="spa24"
                     /locus_tag="ACHU0J_004207"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR1218605.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            complement(1458..>1740)
                     /gene="spa33"
                     /locus_tag="ACHU0J_004208"
                     /gene_synonym="spaO"
     CDS             complement(1458..>1740)
                     /gene="spa33"
                     /locus_tag="ACHU0J_004208"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR1218606.1"
                     /translation="NLSESNGESEHTEVSLALFNYDDINVKVDFILLEKNMTINELKM
                     YVENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISSWMVKE"
BASE COUNT          686 a          331 c          260 g          463 t
ORIGIN      
        1 agtcagaaat gttaagatat ttgagacacg gaaagaacat tgagaaaata acgggcatat
       61 attataagac aattgtatgg attctaaaat aaagaccata ccaccactgt atagaaatac
      121 aactgcagaa aaaagattga aaaattttgc caactcagac gtatcaacac cattggcagg
      181 atcaattgaa ctactaagcg ttgccccacg ctggttgtca ataatgctac caacagcatg
      241 aaatatccaa aagggaagag acaagcaaaa agagagaagg aggccaacaa ttatttcctt
      301 gaacataagg aaataaacat gttcaaaaac agaagaacct atgtctacct taccagaagt
      361 aattaatcct gatgcaacaa gaaaaatcac aggtattcta atagatggag aaattatacc
      421 gttatttaaa aatggaagaa agaaaaataa tggagccaat ctaaaaaaaa cgccgttcag
      481 gagtattaaa aacacatgaa tactttcgaa ccagcttgaa atgtccatca aaccccactc
      541 ttaattaaaa acattatttc atgacaaaaa gacaataaaa cctcaccata ccatccagaa
      601 agaagtagca aagatattga gacacctata agctttatac caaaaggaag tgtctgctct
      661 tggagttgag tcactgtctg taacaaacca atagataatc caataaccgt agctatacct
      721 actggccata aggaaaagat aaggattaaa taaagagcct tattacccat ataaactata
      781 tcagacacat tacctccatc atctaagcag gaatattgat atattgttca atcaaggcct
      841 tagacaggat accccaccca tcaagcgcaa caaataatac aagttttatt ggtactgata
      901 ttgtgattgg actcatcatc atcataccaa gtgcaagtaa aatgcttgaa ataacaagat
      961 caacaacaac aaatggcaaa tataaataaa aacctatttt gaacgcatct ttgatttcac
     1021 ttagcgcata tgcaggcaag agggaaaaca atgaataatc attattctct gcactcttta
     1081 aatcagcatt ttcttcttca gacctttgga aaaaacgtgc taactcaaga tctgtatgct
     1141 ttttcaagta ttgcttatat tccatcaagc cagaatcaga aaaacggaca atatcagata
     1201 ttgtatcaaa tttttgaggg ccatttagat aattttcata accagcttca ataatgggtt
     1261 tcatcacgaa caaagccata atcaatgcaa taccattgag agtcatattt gatggaacct
     1321 gctgcaaacc aagtgcattt cgtaccatta caaaaacaat agaaaacttt atataacagg
     1381 taccagcagc aactaaaaat ggtaatagcg taaaaaaaga aagtgtagcg atgagggaca
     1441 tgtcactcag catgagatta ctcctttacc atccaagaac taatctcgat accataacca
     1501 tcctcaatag aaacaagttc cccatgccca accaaagaac catttacttt aatatttaca
     1561 tgtttaacta tgtcatcggg aaacttgaat aattcgtttt ctacatacat ttttagttca
     1621 ttgattgtca tatttttttc taaaagaata aagtccactt ttacattgat atcatcataa
     1681 ttgaataatg ccaaagaaac ttctgtgtgt tctgactcac cattactttc tgacagatta
//
LOCUS       DAZPDE010000351         1736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPDE010000351 DAZPDE010000000
VERSION     DAZPDE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.358"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHU0J_004209"
     CDS             311..505
                     /locus_tag="ACHU0J_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218607.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHU0J_004210"
     CDS             866..994
                     /locus_tag="ACHU0J_004210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218608.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHU0J_004211"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHU0J_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          519 a          386 c          373 g          458 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag catttt
//
LOCUS       DAZPDE010000352         1733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPDE010000352 DAZPDE010000000
VERSION     DAZPDE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.359"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(9..218)
                     /locus_tag="ACHU0J_004212"
                     /pseudo
     CDS             complement(9..218)
                     /locus_tag="ACHU0J_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(268..519)
                     /locus_tag="ACHU0J_004213"
     CDS             complement(268..519)
                     /locus_tag="ACHU0J_004213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218609.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            971..1255
                     /locus_tag="ACHU0J_004214"
     CDS             971..1255
                     /locus_tag="ACHU0J_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR1218610.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1255..1530
                     /locus_tag="ACHU0J_004215"
     CDS             1255..1530
                     /locus_tag="ACHU0J_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1218611.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          476 a          435 c          407 g          415 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatataggaa aatgtgcctg acagccctaa cagaataagt ttacgcgtaa atcgccctga
      481 atccagaaga gcattaagta tcacataaac aggttccata ccagtcctga ccgcatcaga
      541 acgccagtaa ataaaatctc gtagtccacc ggcatcagag acaagagtaa tcgtcgttac
      601 cggtatttcg actggtttgt actggtgtga gcggacaaaa ctactgtacg tctctcatac
      661 acgctcgtaa gcggccctca cggcccgcta acgcggagat acgccccgac tgcgggtaaa
      721 cccttgtcgg gaccactccg accgcgcaca gaggcatttt catcgctgac aggccgcatg
      781 gcctcgcaga gccggagggg gactggtgat attcggacga ccgtaccggc ttgcgccggg
      841 cttcggcggt tttacacctg tgctacatgt aaccaccgta tgccgcctta tatgcgctga
      901 ctcgcattat ttgccttgtt ccgtctgaaa gtaatcacta tgattaaata tgattaacaa
      961 ctaatcgggt atgcaaatga aaaacaatac cgcacaagca acaaaagtaa ttaccgcgca
     1021 tgtgccatta cctatggctg ataaagtcga ccagatggcc gccagactgg aacgctcccg
     1081 gggctgggtt atcaaacagg cgctttctgc atggcttgcc caagaggagg agcgtaatcg
     1141 cctgacgctg gaagccctgg acgatgtgac atccggacag gttatcgacc atcaggctgt
     1201 acagtcctgg gcggacagcc tcagtactga ccatctgtta ccggtgccac gctgatggaa
     1261 ctgaagtgga ccagtaaggc actttctgat ttggcgcggt tatatgattt tctggtgctg
     1321 gccagtaaac ctgctgccgc cagaacggtg cagtccctga cacaggcgcc ggtcattctg
     1381 ttaactcatc cacgtatggg agaacagttg tttcagtttg aacccaggga ggtcagacgg
     1441 atttttactg gcgagtacga aatccgttac gaacttactg gccagactat ttatgtattg
     1501 cgcctgtggc acacacgaga gaacaggtag attgggtgtg gaggcaactt taacatagcc
     1561 tgcaggtatt aattctggag cattgttatc aggccagata ttttgcattc cattaaataa
     1621 tcttgtagga gataaaataa attacgatga aataacgaaa attaccgctc gtgactggcg
     1681 gcaatatcgc gctccgggct ggcaaataac ccatcagaaa cgttattgcc aga
//
LOCUS       DAZPDE010000353         1731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPDE010000353 DAZPDE010000000
VERSION     DAZPDE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.360"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHU0J_004216"
     CDS             <1..143
                     /locus_tag="ACHU0J_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1218612.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHU0J_004217"
     CDS             127..486
                     /locus_tag="ACHU0J_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218613.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHU0J_004218"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHU0J_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR1218614.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHU0J_004219"
     CDS             707..1141
                     /locus_tag="ACHU0J_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218615.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1731
                     /locus_tag="ACHU0J_004220"
     CDS             1063..>1731
                     /locus_tag="ACHU0J_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1218616.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          568 a          379 c          427 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc g
//
LOCUS       DAZPDE010000354         1729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPDE010000354 DAZPDE010000000
VERSION     DAZPDE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.361"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACHU0J_004221"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACHU0J_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR1218617.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="ACHU0J_004222"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="ACHU0J_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            821..>1729
                     /locus_tag="ACHU0J_004223"
     CDS             821..>1729
                     /locus_tag="ACHU0J_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR1218618.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          446 a          400 c          429 g          454 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggattt
//
LOCUS       DAZPDE010000355         1712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPDE010000355 DAZPDE010000000
VERSION     DAZPDE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.362"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(444..590)
                     /locus_tag="ACHU0J_004224"
                     /pseudo
     CDS             complement(444..590)
                     /locus_tag="ACHU0J_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            644..826
                     /locus_tag="ACHU0J_004225"
     CDS             644..826
                     /locus_tag="ACHU0J_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1218619.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(927..1256)
                     /locus_tag="ACHU0J_004226"
     CDS             complement(927..1256)
                     /locus_tag="ACHU0J_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1218620.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1428..>1712)
                     /locus_tag="ACHU0J_004227"
     CDS             complement(1428..>1712)
                     /locus_tag="ACHU0J_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218621.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          470 a          397 c          402 g          443 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcaaa tagcaatgtc
      121 tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt ccctgaaggc
      181 gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga acaaagctga
      241 ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg gtatccaacc
      301 cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca gggaattaaa
      361 cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt catatcagcg
      421 tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca tggcaggagt
      481 tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg cagatacgct
      541 tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag caggaatctc
      601 agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc acccggtcat
      661 tggagtagta accaaagcag atttagccag catggaacaa atatctttgg tgaaaagttg
      721 gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata acaatggagt
      781 tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc gattggttct
      841 cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga gggttgccgg
      901 gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat ttcgcccata
      961 gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt tttgatgatg
     1021 taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc ctggattgct
     1081 tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag gacgcgcttc
     1141 tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt gttcttacga
     1201 cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt ttccattttt
     1261 atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt tagggccaac
     1321 attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc atacccttta
     1381 tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta tttgcgcaag
     1441 gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat gttcagccac
     1501 acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc gagcaactga
     1561 cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg ctgcggatta
     1621 ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg ctgggtatca
     1681 caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DAZPDE010000356         1694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPDE010000356 DAZPDE010000000
VERSION     DAZPDE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.363"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            189..1187
                     /locus_tag="ACHU0J_004228"
                     /pseudo
     CDS             189..1187
                     /locus_tag="ACHU0J_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1274..>1694)
                     /locus_tag="ACHU0J_004229"
     CDS             complement(1274..>1694)
                     /locus_tag="ACHU0J_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218622.1"
                     /translation="CFFALWKARDGSTTSLECTSTLTRNAKTDHSLYYSANLSVILKK
                     DGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGNAKAKVSGLENQIKDENFRL
                     NFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
BASE COUNT          433 a          361 c          353 g          547 t
ORIGIN      
        1 ccttttgacc gctaaaaaat ggcactcggc acagtaaacc ccgctatagt tatgctatgc
       61 atttgtcttt aatgatatct cgtcgttata ttaacaggct gaaattatca ttaatgcaca
      121 tttcaatatt agcagggata cctgaggagt ttaaagtgaa catatatatc gggtagcttt
      181 tcaaattaat ccctttgatt ataggcttaa tttgcatcgc gttaggtggc tttgtgctgg
      241 aaagttcagg gcaacgtcac gggtcatgtg ctgatttctc tggcggccat atgcctggca
      301 ttattcacta ccgcatttat tatcatttcg caactcacgc gcggcgttaa tacgttttac
      361 aatacattgt tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg
      421 gcactgttag caggtaatga tgtgatggca gacgagtttg ttgccggcca tgttattttt
      481 ggcgttggta tgattgccgc atgtgtatcg acggtggcag cgtcgtccgg tcactttctg
      541 ctcattccca aaaatgcagc ggggagcaag agcgacggaa caccggtaca ggcttattct
      601 tcattaatcg gtaattgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt
      661 tggtctatta cgctgttaca tagtgccgac ataactccgc attatgtcgc cttgggttaa
      721 ccgcaatctg tgcctgtcta attggcctag tggccacaat cgtccatcaa acacgtaata
      781 cgttctcatc caaagagcac tggctgtggt gttattgggt tatttttctc ggctcagtca
      841 cggtgctgca ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg
      901 gcattattct catttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac
      961 tggcactggt ctggagacgt acctgttcgt tagccaacag aataccgatg ataccggtat
     1021 ttacctgtct gttttgcttg tttttgtcat cattcctcgc ggaaatcgca caaactgagc
     1081 tggactactt tattccatct cgggttctgg ttggattagg cgcagtatgt tttacattgt
     1141 tctctattgt ttcaatatta gaggccggtt ctgcgaaaaa aaattaaaat ttacataatc
     1201 ccagcgttat tcatatctta acgctggttt tttacgaact tagtgaaata atgtagaata
     1261 taaaattata attttatatt ggagcacatg tgttaatgat caaccccggg atactaaaaa
     1321 tatagacatt attaaacttg cttagcctcg cattgccttt acctgttaaa gatgcatcga
     1381 gaaaattgag tctgaagttc tcatccttta tctgattttc cagtcccgaa actttggcct
     1441 tagcattacc tgagatccgt ccgttactat ctattttgta ggtaaaaaaa tacgaatggg
     1501 aaaatttcct cggtgtatct tcatcagtta agccaacaat cgtaaaacta ccgctaccat
     1561 ctttttttaa aatgactgaa aggttagcag agtagtataa agaatgatct gttttagcat
     1621 ttcttgttaa cgtcgacgta cattcaagag acgttgtgct accatcacga gccttccaga
     1681 gagcaaaaaa acac
//
LOCUS       DAZPDE010000357         1684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPDE010000357 DAZPDE010000000
VERSION     DAZPDE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.364"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            39..1139
                     /gene="ompC"
                     /locus_tag="ACHU0J_004230"
     CDS             39..1139
                     /gene="ompC"
                     /locus_tag="ACHU0J_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1218623.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1303..>1684
                     /locus_tag="ACHU0J_004231"
     CDS             1303..>1684
                     /locus_tag="ACHU0J_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218624.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPK"
BASE COUNT          512 a          348 c          348 g          476 t
ORIGIN      
        1 gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg
       61 tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc tataacaagg
      121 atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt actgatgata
      181 agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa acgcaaatca
      241 atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat aacgatgaag
      301 ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa attagtgaat
      361 ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt tcatggaccg
      421 atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc atgacatacc
      481 gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt gaagggctga
      541 actttgccgc gcaatatcaa ggcaaaaata aacgtactga caacagtcat ctttatggtg
      601 ctgacgacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat gtttatgatg
      661 gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag gaaagagccg
      721 cagctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca ggtataaagt
      781 atgatgccaa caacatctac tttgcagcta attacgatga aacattaaac atgaccacct
      841 atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg caatatcaat
      901 tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga gatctgggcc
      961 gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat ttcttcaaca
     1021 aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc gactttaccc
     1081 gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat cagttctaac
     1141 gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg ctgtatgact
     1201 ccttaagtaa aaatgagcaa ttcttgatat ttctggtcat tcacctcaat taatattatt
     1261 ccatatcctc cggggacatc ttactataat atttattgat gagtgattat gaccaatatt
     1321 aatacagctt gtgtaaaaaa taatgccagt tatcaactta ataacgcatt acctaacaaa
     1381 gaaaccatct ccagcaattt ttgtgaacga ctagaacaat ggggtaataa atcggttaat
     1441 aatggtgaag aaagagcaat tgccgtggag cgaattaaag aagcttacaa ttcgaacatg
     1501 gcatctcttg atttgtcata tcttgatttg agtgaattac cgcctatccc ctcgacagtt
     1561 aatacgttaa atctggaaaa caattgtcta acttgtcttg acttagctga caatgccagt
     1621 ctcgttaata tcaacctcag cgttaataaa attaacacga taacctttcc aaaagaacca
     1681 aaac
//
LOCUS       DAZPDE010000358         1673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPDE010000358 DAZPDE010000000
VERSION     DAZPDE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.365"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            134..853
                     /locus_tag="ACHU0J_004232"
     CDS             134..853
                     /locus_tag="ACHU0J_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR1218625.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            1285..>1673
                     /locus_tag="ACHU0J_004233"
     CDS             1285..>1673
                     /locus_tag="ACHU0J_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1218626.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFV"
BASE COUNT          530 a          298 c          321 g          524 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgttcggag aggaccctta cgcttacata cattacatat
       61 aatgtatcgt tttataaaag atgataaaca atcaatataa gataatatat ctattttata
      121 gaggacgttt tctatgccca taaaaaagcc ctgtctaaaa ctaaatttag attctttgaa
      181 tgttgtgagg tctgaaatac cgcagatgct ttctgcgaat gaaagattga aaaataactt
      241 caatatattg tacaaccaaa tacggcaata cccagcgtac tatttcaaag tagcatcgaa
      301 cgtgccaact tactctgata tatgtcaatc tttttctgtc atgtaccaag gattccagat
      361 tgtgaaccat agtggagatg tgtttattca tgcatgtcgt gaaaatcctc agagtaaggg
      421 ggattttgtt ggggacaagt ttcatattag tatagctagg gagcaagttc ctctggcatt
      481 ccaaattttg tctggtttat tattttcaga ggacagtcct atagataaat ggaagataac
      541 tgatatgaat cgagtttctc agcaatctcg tgtggggata ggagctcagt ttacgctata
      601 tgtaaaatca gatcaggaat gctcgcaata tagtgcttta ttacttcata aaatacgaca
      661 atttataatg tgtctcgagt ctaatctatt aagaagcaaa atcgctcccg gggagtatcc
      721 ggcgtcagat gttagacctg aagactggaa atatgtcagc tatcgtaatg aactacgaag
      781 cgatcgagat ggaagtgaaa ggcaagagca aatgttacga gaggaaccat tttatcgttt
      841 gatgatagag tagagaagat gccagctaca ccaccttgta gcttgaaaac tggttagaat
      901 aactacgatt agatttggca ctgcgtatgc agcctgattc ctgcccgtga cagagcattc
      961 ggctcataaa ctgctgagct actcatctga aatccgaaca gttgtccacc atttcagcgt
     1021 gcatctcctg tttgagtcgt ttgattgctt ttcgggatat taacctgtcg gattttgcat
     1081 tagttaatgc tcccatcagg ccttcatctt ttccagtgcc tattttcctg ttctcctttt
     1141 gcacaatata atcagatcat ctgtatcgtt gacgagctcg ctatcttata ggagcattct
     1201 gaataagcta cctccttgta aggaggcttt tactattagt aactgcaatc taatagtatt
     1261 ataacatctc tatggttgtc ttctatgccg ttaaataaaa cattttcatc tagtatattt
     1321 tcaacaaaaa attcgttatc aacagatatg tctgttaaca gagataatag aacaataaca
     1381 agttctatta tgagggtctc aaactccagt gaactaattc agtttaagaa taaaacagca
     1441 ccttattttt cagaaaagag aaatgtggag gtaaatataa atggagtggc aaaagatata
     1501 tatggaagac aaatagtttg ccgtcaccta gcttcctatt gggaaatgaa ttttatggaa
     1561 acaaatggga aggtaaacta tcagcttctt agcacccctg atgctattgc aaaaaatgta
     1621 tgtttggaaa aaacggaaga tttttcaaaa tcaccagctt atatttattt tgt
//
LOCUS       DAZPDE010000359         1636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPDE010000359 DAZPDE010000000
VERSION     DAZPDE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.366"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            17..667
                     /gene="zinT"
                     /locus_tag="ACHU0J_004234"
     CDS             17..667
                     /gene="zinT"
                     /locus_tag="ACHU0J_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1218627.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          523 a          318 c          324 g          471 t
ORIGIN      
        1 tctggaggaa attgttttgg cgattcgtct tcataaactg gctgttgctt taggtgtctt
       61 tattgttagc gctcctgcct tttcgcatgg tcatcattct catggcaaac ccttaacaga
      121 ggtcgaacaa aaagctgcta atggtgtttt tgatgatacc aatgtacaaa accgaacgct
      181 cagtgactgg gatggagtct ggcaatccgt ttacccttta ctgcaaagtg gcaaacttga
      241 tcccgtcttt cagaagaaag cggatgcaga taaaaccaaa acatttgctg aaattaaaga
      301 ttattatcgc aaaggttatc cgacagatat cgaaatgatt ggcattgagg acggcattgt
      361 cgaattccat agaaataatg aaacaacatc ctgtaaatat gattacgatg gatacaaaat
      421 actcacctat aaatcaggca agaaaggcgt tcgttactta ttcgaatgta aagatcctga
      481 aagcaaagcc cctaaatata tacaatttag cgatcatata attgcaccac gaaaatcatc
      541 tcattttcac atttttatgg gtaatgattc ccagcaatct ttattgaatg aaatggaaaa
      601 ctggccaacg tattatccct atcagttgag tagcgaagaa gtggtcgagg agatgatgtc
      661 tcattgattt ttttgcgctg acttcagtta tttaacagaa cagtgcctgg tagtaacaac
      721 aataatttac caagacaaat aagtaagaca aactcagaca cgctcatact tgagtcgtgt
      781 cttttttatt atgtctgtag aaaaaaactg agaaaaaggc taacaataaa tacacaactc
      841 agccatacag actggcagcg caaatgtttc caggagcaag tcatctgtca tggtaaagct
      901 aaccagaaat ataggttaat tttggtgttt aaaccttttt tacttgttag tatgatacaa
      961 aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact ttgtcccgcg
     1021 gctttaatct gacggccgcg ttcctttttt ggctcaatta ttgacccctt ctctacgcaa
     1081 cttcagttcc cgccagcaac tttgcggcag tttcatagga tcaatgtcta aaagaataat
     1141 ggtgaccgat aagaaaacga ctgaataact gtagattttc gctcgaaacc ttcctgtcag
     1201 ctccatagcg aatcaagcgc tgaatgtcac agtatcgaac agaaaacagt gacgatctaa
     1261 cccttcaaga atattctacg attgttctgt ttaggaaaag caaggcggga agtcgggaga
     1321 taagtcattg ataaaatggc ggagagaggg ggatttgaac ccccggtaga gttgccccta
     1381 ctccggtttt cgagaccgat gatacagagt cgaaaaatca aagagttaaa aatttttctt
     1441 ggaaaaaatg tcttaaaatc gtacacgaaa aatcaatgaa ttacataaca ttaaagaaga
     1501 atattccaag ccttcttgag tccaccacct gttttcaggt gtcattcaga cgtgatttgc
     1561 cccagattgg gaaagcacgc ttttttctca gcttattccg ttttgcctgt gtgttgaata
     1621 attccactgc agttat
//
LOCUS       DAZPDE010000360         1582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPDE010000360 DAZPDE010000000
VERSION     DAZPDE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.367"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(571..1500)
                     /gene="virB"
                     /locus_tag="ACHU0J_004235"
     CDS             complement(571..1500)
                     /gene="virB"
                     /locus_tag="ACHU0J_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR1218628.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT          455 a          243 c          281 g          603 t
ORIGIN      
        1 cttttggggt tagaaaaata aatctcttgt taataattaa tatgatttta aaatcaaact
       61 tgtttataat tataaaataa tgtaagtcgg tcattttatg ggaagattag gagaataaaa
      121 cagtaattat atttgagaaa tctgctaata tattcttaaa gcttttttga tactgtgaac
      181 gaagtctgtt aatggtggat tttttgttta tcaccatgta aaaaaaattg agtagtaaaa
      241 gcgttgactg ctaatcctcg ctgtttttat tgaaacgatt aaaaatacgc tgaatatttt
      301 cggccagtca ctcatattca cataaaattt tgaccatcat gccgcatccc ttagttgcac
      361 aattcatttt caatgatatg aatattacta atttcaccag ataattttct cgtggtcagc
      421 agggtatgag ttttcaccgt aattttaagg aaacacagtg cgtggggatt gaataacaga
      481 tagccgacgg aattacccca ccggctgaaa gtcaagttca acgaagattt ttattatctg
      541 aattgggcag tttacatcag tgttcgatgt ttatgaagac gatagatggc gagaaattat
      601 atcccgaata gcttcatcta tttctctctg aactgttttg tttattttag ataatgtaaa
      661 tattagagtt ctgtttgttt tattctcttt tctttttatg taagtccgtt tgtctttaga
      721 aataaataac gagtcaactt ttagcgaagg gttttgcttt ctgtttttgc ttttctttat
      781 gatgtttaaa atttcttttt tatatatgtc cgggggcaaa tttgtatcaa ggtcttttat
      841 ttcttccttt aatattggta gtgtagaact aagagattca ttagcctttt caagtccttt
      901 ataataattg aataatatct tgtagtcatt aaagttaagt tctgacgcga ttggaaatag
      961 agatattatt tcctgtggaa cgcttgctgc ctgaaaggca cgagtgactt tcgcgcgaga
     1021 cagattctct tttttggcta tgtctttata ggacatccct gatactttca gaaaattaag
     1081 accaatacca agttctcgga tgctatgctc ttttgctgtt tgtatatcgt ttgctagttt
     1141 tctggcatca agagtagata tatattcttt tgaatataga acttcaagat ctgctcctgc
     1201 atatattgca gatgctcttc tacgagtgcc atccagaatt tcaattctac catcaatctc
     1261 ccttcctatt acagggaaga attgttgtag ctttatagtt tttatgatat cggctaatga
     1321 ttcttctgtt agggattctt gatcgcgtac attcgttttt tggttgacga aggttaaatc
     1381 ttggatcctt ttatgaggaa tcttggcttt gataaaggaa actttattac cagaatggag
     1441 tgtaaactct ttcttttggc cttcctttat acttaacaag tcgttgcaca aatccaccat
     1501 atcacaccct gtttattcat attgatttat attgacaaaa gttaaatgca gtgtgagcaa
     1561 gattgtatgt aactgttgat ga
//
LOCUS       DAZPDE010000361         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPDE010000361 DAZPDE010000000
VERSION     DAZPDE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.368"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHU0J_004236"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHU0J_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR1218629.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHU0J_004237"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHU0J_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR1218630.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZPDE010000362         1573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPDE010000362 DAZPDE010000000
VERSION     DAZPDE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.369"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHU0J_004238"
     CDS             7..657
                     /locus_tag="ACHU0J_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218631.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHU0J_004239"
     CDS             629..1063
                     /locus_tag="ACHU0J_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1218632.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          446 a          319 c          374 g          434 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agc
//
LOCUS       DAZPDE010000363         1561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPDE010000363 DAZPDE010000000
VERSION     DAZPDE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.370"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            400..1149
                     /gene="phoC"
                     /locus_tag="ACHU0J_004240"
     CDS             400..1149
                     /gene="phoC"
                     /locus_tag="ACHU0J_004240"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR1218633.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1204..1308)
                     /locus_tag="ACHU0J_004241"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="ACHU0J_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1319..>1561)
                     /locus_tag="ACHU0J_004242"
     CDS             complement(1319..>1561)
                     /locus_tag="ACHU0J_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1218634.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          426 a          332 c          316 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAZPDE010000364         1530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPDE010000364 DAZPDE010000000
VERSION     DAZPDE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.371"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1459
                     /gene="ltrA"
                     /locus_tag="ACHU0J_004243"
     CDS             <1..1459
                     /gene="ltrA"
                     /locus_tag="ACHU0J_004243"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1218635.1"
                     /translation="QRLLRLITQPEWLAEAARITLSSKGAHTPGVDGVNKTMLQARLA
                     VELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALRDRIVQRAMLMAMEPIWE
                     SDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEGDLSSYFDTVHHRLLMKA
                     VRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLH
                     ERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAE
                     AIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQE
                     KARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFW
                     KLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKK
                     LFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          391 a          379 c          442 g          318 t
ORIGIN      
        1 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
       61 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      121 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      181 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      241 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
      301 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
      361 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
      421 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
      481 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
      541 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
      601 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
      661 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
      721 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
      781 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
      841 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
      901 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
      961 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1021 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1081 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1141 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1201 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1261 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     1321 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     1381 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     1441 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     1501 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAZPDE010000365         1529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPDE010000365 DAZPDE010000000
VERSION     DAZPDE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.372"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            526..695
                     /locus_tag="ACHU0J_004244"
                     /pseudo
     CDS             526..695
                     /locus_tag="ACHU0J_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            841..>1529
                     /locus_tag="ACHU0J_004245"
     CDS             841..>1529
                     /locus_tag="ACHU0J_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1218636.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          377 a          347 c          466 g          339 t
ORIGIN      
        1 atctgttctc caatgacaag tctaaaaact ggtattaaga ctatcactta aataagtgat
       61 actggttgtc tggagattca gggggccagt ctagatgcca acacaaaggc aactaataaa
      121 gatacagata aagatctaaa aacagatcta acccatccca aacccttccc ttccggaagg
      181 gagtttcggg attttgtggc tggagtgctt gaggggagat tatctggcgg tactgcagcg
      241 gaattttgta attctgcggt ggttgcgttg caggctgctg gcctggatgt ctgtcgtgag
      301 tatccggtgc cagagcgtgg tgacggttgc ggagggcgga ttgatatcgt cgtgactgac
      361 aggaacggtg tccggtgtgg gatcgagctt gaccgaaatt ctccgcgaca gaaatcactg
      421 ctcaaaatcg gtgctgttga aaccgggata tgtgtcttgc ggcgcagtga tatcgcaagg
      481 cacaccgagc agggaattct ggttatcggt ggggctgttc gccagaaaaa atttgacccg
      541 ttgtcagttg atctgcccga ctggttgtca gaaacactct ggcatgagtg ggtccagttc
      601 aggcaggcat tgcgaaaacc gattcgaacg gagctgggcg ctaacggggc gatacgggaa
      661 ctggaaaaat tccgtcagca gggttttaca cctgagcagg tgattcgaca cagcatcgcc
      721 aatgaatacc agggtctgtt cgcgccgaaa ggtgttcggc ctgagacgtt gctccgacag
      781 gttaacaccg tctcgttgcc ggacagtgcg atcccgccag gcttcagggg gtaacagacc
      841 atgaaaaata ttgcgacagg aggcgttctg gagcgtatcc gcagactgac cccaccacat
      901 gtaaccgccc cattcagaac ggttgcggag tggcgcgagt ggcaacttgc tgaaggccag
      961 aaacgttgcg aggagatcaa ccgcctgaat cgtcagttgc gggtggaaaa aatcctgaac
     1021 cgctccggca tccagccgtt gcaccggaag tgttcgtttg cgaattaccg ggcgcagaac
     1081 gacggtcagc gacatgcact gagtcaggcg aaatccatag ctgacgaact gatgacaggc
     1141 tgtacgaatt ttgtgttcag cggtaagccc ggcaccggaa aaaatcatct tgcagccgcg
     1201 attggcaacc ggctgatggc gaaggggcgt agcgtgatta tcgtcaccgt gtccgatgtc
     1261 atgagcgtgt tgcatgagag ctacgacaac ggcaaatccg gtgaaaaatt tttacaggag
     1321 ctttgtggtg ttgacctgct ggtcctggat gaaattggca tgcagcggga tacgaaaaac
     1381 gagcaggtgg tactgaacca gattgttgat cgccggacgg catcgttacg cggtgtgggg
     1441 atgctgacaa atattaacca tgcagcgatg aatacacttc tcggcgagcg ggtgatggat
     1501 cgcatggtca tgaacggcgg gcgctgggt
//
LOCUS       DAZPDE010000366         1505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPDE010000366 DAZPDE010000000
VERSION     DAZPDE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.373"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            223..351
                     /locus_tag="ACHU0J_004246"
                     /pseudo
     CDS             223..351
                     /locus_tag="ACHU0J_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            541..945
                     /locus_tag="ACHU0J_004247"
     CDS             541..945
                     /locus_tag="ACHU0J_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218637.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1008..1505
                     /locus_tag="ACHU0J_004248"
                     /pseudo
     CDS             1008..1505
                     /locus_tag="ACHU0J_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          423 a          362 c          399 g          321 t
ORIGIN      
        1 cacaaagtga attaaatgaa tatataatgc gttcgaagta tatgtattga gctgtcatac
       61 tgagaaacaa accttagggc agcacaatta aagccagaaa caactatgca agaaagttac
      121 taaataagtg ctcggaacag taaaacactc aggggggact cagtccggat tatgtaatca
      181 gataaattgc caaacatcac aaatcaccag ccggactgag tgatgccgag cattgcacca
      241 ataccccgtg atgaacgatg cctgatgcag aaagctccct ataaaacaaa gaacaaaaac
      301 tacgaccggc gattcaccgt catactgatg ctgcatcgtg gtgacactgt ctgtcacttt
      361 catgttgact gaacaactct gctgcgaaga aggtgtaccg ccggcgggat ttgattgtcc
      421 ggcgtctagc ttccacttcc ctgatggaac tgaaagttgt tatggccggt cagcttgccc
      481 tgtccgcagg acatgaggtg ctgtgacgga cagcaccagt aacggaaagt gacaatgaaa
      541 atgaatactg cgctgatagt tgcactaatg tgtatgtggt atgcggtacc cgctgctgca
      601 aaagagactt tactggctat gccacgtaat tcaacagaac attgctatgc ggagattaat
      661 gtgcacggac catatggggt gtatttccga gtggtcccgc atccccccgg gggaaagagc
      721 tgggtcgaat gtaacagtga ctactattac tctgataaac caccgggagt acagatactc
      781 ggaacccgtg cagggtgtcg ggtatacgga atctgcggga ccacctcgac actgcatgtg
      841 gctggaagag gggttgtgtg tattaaaaat atatgcagtc cacgtgggat gataattcac
      901 aggatacgga aacgtcctgt agtggctgta agcgatgaga tgtgatctta cccagcaata
      961 gtagacacgc ggctaagtga gtaaactctc agtcagaggt gactcacatg acaaaaacag
     1021 tatcaaccag caaaaaaaca cgtaaacagc attcgcctga attttgcagt gaagccctga
     1081 agcttgctga acgcatcggt gttgctgccg cagcccgtga actcagcctg tatgaatctc
     1141 agctctatgc gtggcgcagc aaactgcagc aacagatgac ctcgtctgag cgtgaaagtg
     1201 aactgcccgc ctgaagcggc aactggcgga gcaggcagag gaactggcta tcctccaaaa
     1261 ggccgcgaca tactttgcgc agcggctggt atacgtggtg tcagcgtcgg acaggaataa
     1321 gccctcgtca gcagttccgt cagcactgcg acagcgttgt cctcgcggcg gcctttaccc
     1381 ggtcaaaaca gcgttacggt gcaccacgcc tgacggatga actgcgtgct cagggttacc
     1441 actttaacgt aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct
     1501 cccgg
//
LOCUS       DAZPDE010000367         1497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPDE010000367 DAZPDE010000000
VERSION     DAZPDE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.374"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHU0J_004249"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHU0J_004249"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1218638.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          404 a          310 c          407 g          376 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctg
//
LOCUS       DAZPDE010000368         1494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPDE010000368 DAZPDE010000000
VERSION     DAZPDE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.375"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHU0J_004250"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHU0J_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1218639.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
BASE COUNT          445 a          275 c          298 g          476 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gcta
//
LOCUS       DAZPDE010000369         1447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPDE010000369 DAZPDE010000000
VERSION     DAZPDE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.376"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHU0J_004251"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHU0J_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHU0J_004252"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHU0J_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1218640.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          408 a          326 c          297 g          416 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgc
//
LOCUS       DAZPDE010000370         1414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPDE010000370 DAZPDE010000000
VERSION     DAZPDE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.377"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHU0J_004253"
     CDS             <1..294
                     /locus_tag="ACHU0J_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1218641.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACHU0J_004254"
     CDS             300..1397
                     /locus_tag="ACHU0J_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1218642.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          438 a          320 c          347 g          309 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttgg
//
LOCUS       DAZPDE010000371         1360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPDE010000371 DAZPDE010000000
VERSION     DAZPDE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.378"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            104..844
                     /gene="phoN2"
                     /locus_tag="ACHU0J_004255"
     CDS             104..844
                     /gene="phoN2"
                     /locus_tag="ACHU0J_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR1218643.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            917..1039
                     /locus_tag="ACHU0J_004256"
     CDS             917..1039
                     /locus_tag="ACHU0J_004256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218644.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1197..1360)
                     /locus_tag="ACHU0J_004257"
                     /pseudo
     CDS             complement(1197..1360)
                     /locus_tag="ACHU0J_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          404 a          291 c          266 g          399 t
ORIGIN      
        1 tcactctgtt caaatttttc cgcatgactt gtgttttttg taatacagct cgttttttac
       61 atctgaccaa aatcatcaat taattatgct aaggaaataa attatgaaaa ccaaaaactt
      121 tcttcttttt tgtattgcta caaatatgat ttttatcccc tcagcaaatg ctctgaaggc
      181 agaaggtttt ctcactcaac aaacttcacc agacagtttg tcaatacttc cgccgcctcc
      241 ggcagagaat tcagtagtat ttcaggctga caaagctcat tatgaattcg gccgctcgct
      301 ccgggatgct aatcgtgtac gtctcgctag cgaagatgca tactacgaga attttggtct
      361 tgcattttca gatgcttatg gcatggatat ttcaagggaa aataccccaa tcttatatca
      421 gttgttaaca caagtactac aggatagcca tgattacgcc gtgcgtaacg ccaaagaata
      481 ttataaaaga gttcgtccat tcgttattta taaagacgca acctgtacac ctgataaaga
      541 tgagaaaatg gctatcactg gctcttatcc ctctggtcat gcatcctttg gttgggcagt
      601 agcactgata cttgcggaga ttaatcctca acgtaaagcg gaaatacttc gacgtggata
      661 tgagtttgga gaaagtcggg tcatctgcgg tgcgcattgg caaagcgatg tagaggctgg
      721 gcgtttaatg ggagcatcgg ttgttgcagt acttcataat acacctgaat ttaccaaaag
      781 ccttagcgaa gccaaaaaag agtttgaaga attaaatact cctaccaatg aactgacccc
      841 ataaagctgg acagcctgta tcaggctatg gagggcccat agacaaatct accctatatg
      901 agcataggag gagtctatgg gcacaccacg ttttacccct gaatttaagg gattactgga
      961 aaggctggga catatccctc cggcagaagc agaaaaagct tattatgctg ccatcggaaa
     1021 cgatgatctg gcaacctgag ttcacagata aaacattctc taggaaactc ggggcggttc
     1081 cgttcaccac atgcaatgtg gtgttgcagg ggaacggtct gcaccatccc ctatgtcgat
     1141 caatataaca gaaatgacaa cttcagattc agggcacaac ctaaatatat tttaggtcac
     1201 ctctcaaatc gtttgcctga taccgctcca ttttttaata aaaaaatcaa tcatttttga
     1261 atctttgctt ttcattgcat tgtcaagcat agtgtcccca ctatttgctt tttgaaattt
     1321 tttgtttaca tcaaccaatc cattattgat aaaatattct
//
LOCUS       DAZPDE010000372         1356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPDE010000372 DAZPDE010000000
VERSION     DAZPDE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.379"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHU0J_004258"
     CDS             complement(41..100)
                     /locus_tag="ACHU0J_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1218645.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHU0J_004259"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHU0J_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            complement(1281..>1356)
                     /locus_tag="ACHU0J_004260"
     CDS             complement(1281..>1356)
                     /locus_tag="ACHU0J_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1218646.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAZPDE010000373         1356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPDE010000373 DAZPDE010000000
VERSION     DAZPDE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.380"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..420)
                     /locus_tag="ACHU0J_004261"
                     /pseudo
     CDS             complement(1..420)
                     /locus_tag="ACHU0J_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(442..982)
                     /gene="yhaB"
                     /locus_tag="ACHU0J_004262"
                     /pseudo
     CDS             complement(442..982)
                     /gene="yhaB"
                     /locus_tag="ACHU0J_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(1238..>1356)
                     /locus_tag="ACHU0J_004263"
     CDS             complement(1238..>1356)
                     /locus_tag="ACHU0J_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR1218647.1"
                     /translation="FYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          418 a          235 c          253 g          450 t
ORIGIN      
        1 gttcaacctc ctgatttcat tgtaaatatc ttttaatggg caattaacat tgatattgcc
       61 tgtagatatt tcctagtttt ttaacggttc attaatggta agttcgcagg tattgtccga
      121 tcgttgaata atttgggtgt tataacctaa aaaatccaga cgaagagacg agtcattata
      181 aagaattaca ttttgtaagt acgctaattc tttttgaaaa tcaccagtga taatgcattt
      241 tccacttggt tgtaacgcgt taagtaaagt gaccatttga atagaggtct gttttagttt
      301 ttctatacct ggtgctagtg cttcgaggtt atttcctatg gggctttcag gaaggtcatt
      361 catttttcga cggactaaat cactactaat atcattacca attgatgaaa cagggaacat
      421 tattttatcc tttataaata atcaataaaa atcattatta aaatgatcgg gtgaaaaaaa
      481 gagaatttgc caacaatctg aagtaatgca ggttttatta aagtgcctag ggggtgttaa
      541 gatggcttct ttttgggtga actttgcgca atacttagaa atcgtctcct taatacattc
      601 cagctctttt ttattgacgg ctgagcaagt acattgttca ccaaaaggtg caaaagtgcc
      661 tacatctaat gtttgtatga tataatcatc atgttctcct gtagaaatat ctatgatgaa
      721 tggactcata ttttccggtt ggattcggaa tcatgacaga aggaacatcg ccttgataga
      781 aattcaatgc agtaattgca gataatactc tccggagttc attacttata ctgtaaattt
      841 tattaacgtc attggtggag acaatttctg caaactttct ttttaccgtc caataatcaa
      901 tatttctatt gtgattgtta accactgcgt gcattggcgg gattgaaggg tgaaaaatat
      961 gtgcaattgg gaagcctttc attgatgcct gccttgtcgt catgacaaac aataatagat
     1021 gcttattttc ctcgcacgac acttacgcac tatcaaaaaa cgctcattta cccggcgtta
     1081 cagtaaacga agcaacattc tgtgtcctac gctgatgtaa agcacagtga aaatccccct
     1141 tgttgacttc gtctgacctt atcgcggcga ccagcaatta ttcgttttgt gatcctcaac
     1201 acaaaatgct attcgtcgcc tatgagggga gaagaattta tggaaaacga ttgcattcac
     1261 aaaatgacgt cctgtcagaa gagaaaaagt tgacgttaat caattgttcg agatgatcaa
     1321 tacctgtatt cgaaatttta aaatattgat aaaata
//
LOCUS       DAZPDE010000374         1351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPDE010000374 DAZPDE010000000
VERSION     DAZPDE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.381"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHU0J_004264"
     CDS             <1..243
                     /locus_tag="ACHU0J_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1218648.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>1351
                     /locus_tag="ACHU0J_004265"
     CDS             358..>1351
                     /locus_tag="ACHU0J_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1218649.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          373 a          338 c          361 g          279 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tgcggggatt
      781 gtgtggcgaa gggctgcgcc aactctgcgt atccgtgacc cgcataaaga tgaaaagatg
      841 gcagcaatcc ataaagcact ggacgaatgc agcgcagagc atccggtctt ttatgaagat
      901 gaagtggata tccatcttca tcccaaaatc ggtgcggact ggcaactgcg cggacagcaa
      961 aaacgggtgg tcacgccggg acagaatgaa aaatattatc tggccggagc gctgcacagc
     1021 gggacaggta aagtcagcta tgtgggcggc aacagcaaaa gttcggcgct gttcatcagc
     1081 ctgctgaagc ggcttaaagc gacataccgt cgtgcgaaaa ccatcacgct gatcgtggac
     1141 aactacatta tccacaaaag ccgggaaaca cagagctggc tgaaggagaa cccgaagttc
     1201 aggggcattt atcagccggt ttactcgcca tgggtgaatc atgttgaacg gctatggcag
     1261 gcacttcacg acacaataac gcgtaatcat cagtgccgct caatgtggca actgttgaaa
     1321 aaagttcgcc attttatgga aaccgtcagc c
//
LOCUS       DAZPDE010000375         1324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPDE010000375 DAZPDE010000000
VERSION     DAZPDE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.382"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(419..1042)
                     /locus_tag="ACHU0J_004266"
     CDS             complement(419..1042)
                     /locus_tag="ACHU0J_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1218650.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          362 a          271 c          265 g          426 t
ORIGIN      
        1 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
       61 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
      121 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
      181 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
      241 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
      301 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
      361 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
      421 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
      481 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
      541 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
      601 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
      661 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
      721 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
      781 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
      841 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
      901 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
      961 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
     1021 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
     1081 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
     1141 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
     1201 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
     1261 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
     1321 tttt
//
LOCUS       DAZPDE010000376         1318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPDE010000376 DAZPDE010000000
VERSION     DAZPDE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.383"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHU0J_004267"
     CDS             complement(<1..143)
                     /locus_tag="ACHU0J_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR1218651.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
     gene            complement(545..1306)
                     /gene="ydeO"
                     /locus_tag="ACHU0J_004268"
     CDS             complement(545..1306)
                     /gene="ydeO"
                     /locus_tag="ACHU0J_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1218652.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT          416 a          246 c          248 g          408 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aaagcgttga cattactttc tgttctatta
      301 agtaatttct cgccgataaa caactaattt attgatattt aataaattat tgcattttac
      361 tgacaaaatg ccgaattgag atcataaata atcatgcaac aggttatgca agtgcataaa
      421 tatgtgatgg atgtcactta tttatttcaa taaatatatc gcctaaaaac aacgcggggc
      481 agggaatggc tgccccatat aattcttacg tagcgtgcgt ggttgactac tcgttagcaa
      541 ataatcaaat agctaaagca ctcatcgtgt tgcccgtatt catacccgtg tgcctttgac
      601 aacggtactc cttagaaact ctcttcggac tgttgccgaa atgtttgcgg aacgcataaa
      661 taaaataaga tgtactggca taaccacatt gttcggcaat tttattgact gaaccttcta
      721 cgcgtatcaa attttttgcg tgctgcattc tggcatctaa tagaatctgt gagaatgtcg
      781 tttgctcttg ctttagtttt ttcttcaaca ggctttcact gatgtacagg cagtcacaaa
      841 tatctttcag cttccatggg tgcgccagct tcatgttgac aatatttctc actttcccgg
      901 aaacggatag cacaccgtta gttaatagtg taatgaaacc tttgcatgcg gcaaaaatag
      961 acaggcaaga aaaaagcaaa agttctgaaa aattatgatg attgcgttct tccgagttga
     1021 gataagcaat catttcatta agcaatccag tcggtacatt tgaagcacgt aaaaacttcg
     1081 caggaacagg caccgtcact gtctggatgt tagtgcattg tagatatttt ttaatggtat
     1141 tttcattaaa tccgatttct ttaaaatgcc gctcataagg ttcaaaatgt gtacgaacag
     1201 cgttatctac catcaggatc tcgccgtcgg agaaggcgta atctttatct aaaatgttag
     1261 cgttgaaagc atgatgaata aatataacag aacaaacgag cgacatttta tctcctta
//
LOCUS       DAZPDE010000377         1301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPDE010000377 DAZPDE010000000
VERSION     DAZPDE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.384"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1138
                     /locus_tag="ACHU0J_004269"
     CDS             <1..1138
                     /locus_tag="ACHU0J_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR1218653.1"
                     /translation="HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIA
                     NMWLSKVVDDEGKEIFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPEL
                     LSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNS
                     DGLLKEVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGG
                     WAAEAIEKNPPCKNDVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPC
                     WNCKSGKDRTGMQDAEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQ
                     EMNTGVPGNKVMKKLPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            1148..>1301
                     /gene="ipgE"
                     /locus_tag="ACHU0J_004270"
     CDS             1148..>1301
                     /gene="ipgE"
                     /locus_tag="ACHU0J_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR1218654.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALP"
BASE COUNT          459 a          163 c          304 g          375 t
ORIGIN      
        1 tcatgggaaa aattatggtt ttcaattaac tcctgcctct catatgaaga tagggaataa
       61 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
      121 tgatcatatc gcgaatatgt ggctttcaaa agtggtggat gatgagggga aggaaatttt
      181 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
      241 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
      301 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
      361 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
      421 cgcattaaaa gggttaaact ctaaaagggg gaagccaacg aaattattaa ttcggaatag
      481 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
      541 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
      601 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
      661 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
      721 gataaaagag attgtaacca aaaagttaca aaaaaatgat aatggagagc cgtataaatt
      781 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
      841 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
      901 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
      961 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
     1021 gcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
     1081 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
     1141 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
     1201 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
     1261 agattcaata aatttattat gccctttttg tgcattgcct g
//
LOCUS       DAZPDE010000378         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPDE010000378 DAZPDE010000000
VERSION     DAZPDE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.385"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            132..734
                     /gene="ais"
                     /locus_tag="ACHU0J_004271"
     CDS             132..734
                     /gene="ais"
                     /locus_tag="ACHU0J_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1218655.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(773..1198)
                     /gene="nudI"
                     /locus_tag="ACHU0J_004272"
     CDS             complement(773..1198)
                     /gene="nudI"
                     /locus_tag="ACHU0J_004272"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1218656.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          360 a          287 c          274 g          373 t
ORIGIN      
        1 tggcaaaaat ttgaggcatt ttgccgacat cgtaggattt ttaatattac accaactgct
       61 aattatcgcc agaaatgtcg ctcaatttca cggtaattgt ctggttgcgc ttgtctatag
      121 gtggagttta cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa tatttcatca
      181 ttttactggc gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg
      241 gtttgccacg tatcgacaat aaaacattgg ccagactggc acagcagcac ccggttgtcg
      301 ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa
      361 caggtattac ggttaaaggt acccaggatg cccgtgaact gggcaacgct tttagcgctg
      421 acatctctga tttcgatctt tattccagta ataccgtccg gaccattcag tcggcgacct
      481 ggttttccgc gggtaaaaag ctgacggtag ataaacgact tcttcagtgc ggtaatgaga
      541 tttatagtgc aattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca
      601 cccataatca ttgcctgaca tatattgcta aaaataagcg tgatgcgata tttaaacctg
      661 attatctgga tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat
      721 tcgttaacca ctaaattttc aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa
      781 gacctttcaa acgtaacgtt tttcgggtgg caacatttaa atcataatgc accagatctt
      841 caggttttac ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag
      901 aaacgcagtc aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatatg
      961 tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt
     1021 ctcccagttc ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccagactcca
     1081 cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttgc
     1141 acagcaaata agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcacag
     1201 gggtattcct tataaataaa aagaaaccat gagaatacgg taactcagaa atatgtgaaa
     1261 gtgacagtaa tctcttttgc aatgataact gcac
//
LOCUS       DAZPDE010000379         1285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPDE010000379 DAZPDE010000000
VERSION     DAZPDE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.386"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            113..1252
                     /gene="pdeF"
                     /locus_tag="ACHU0J_004273"
                     /pseudo
     CDS             113..1252
                     /gene="pdeF"
                     /locus_tag="ACHU0J_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT          290 a          286 c          291 g          418 t
ORIGIN      
        1 gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag tcaggtggga
       61 tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat ctatgaaact
      121 gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt tcctgccttc
      181 tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt ccggtgaggt
      241 ttttctcttt tatctgcctt tggcactgat gatcagcatg atgatgtttt tcagctgggc
      301 ggcattgcca gggatcacct tagggatttt tgtccgcaaa tatgcagagc tgggttttta
      361 cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt ggggcggtta
      421 cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt taatttccca
      481 gcgtatattc tggcagatag tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
      541 tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc cttttaacct
      601 cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg tcccgctgtg
      661 ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt attcgcaatt
      721 aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc tactggcatt
      781 aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac
      841 caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcaatgc gctatggtta
      901 taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca gctatcaaaa
      961 ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct ccagttatct
     1021 ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc gagcggtagt
     1081 cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa atgttcgcgc
     1141 cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac gcatccctgg
     1201 catggaaatg ctggttaaga actatggcat catgctgcgg attcaatatg aggtgtactg
     1261 gcaatagcgg acactaccat ttgtt
//
LOCUS       DAZPDE010000380         1284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPDE010000380 DAZPDE010000000
VERSION     DAZPDE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.387"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(133..399)
                     /gene="ospE2"
                     /locus_tag="ACHU0J_004274"
     CDS             complement(133..399)
                     /gene="ospE2"
                     /locus_tag="ACHU0J_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR1218657.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(581..982)
                     /locus_tag="ACHU0J_004275"
                     /pseudo
     CDS             complement(581..982)
                     /locus_tag="ACHU0J_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          339 a          265 c          262 g          418 t
ORIGIN      
        1 atcgaggata gatggagtta ctcctctctg aaatagaatt actgctctac tttttctgtt
       61 tccagaaatt attagtaaca aaccctgata tattcttttg aaaatggagc ttaaacgagt
      121 ctgaaattaa cttcagaaat cagaataccg ttggctttgt ttttcatcaa ttccggcttc
      181 atttatcttt gtcaaccatt gcatttctgt agcagtgaat ttcaacgttt tcgtccggtt
      241 ggcagcaggt atttgtaatt cgtatatagc tgctttttta ttgaaaacag tatatccatc
      301 tgtagtaaca gtagaagata ataatacagg atttgatact tcaaggatga tattttcttg
      361 tttttgtgga aggcaaggga atatagtttg tgtaagcata ttgcttcctt atgttatatt
      421 ttagtaaaga atttattttt tacattatag tcggctaaat gaagtttact gtaaaaaaac
      481 gatacttttt tcgtaagcga tgagatgtgg agccagaaaa atggctgcgt tacgtcattg
      541 aactgtcctt gacccacgat attcagacag tcgttgtatc ttaagttgac attacccgct
      601 gacgtcgata ttcccttggt gagagataac ccaacgcact atgcggatgg ttttcattgt
      661 aatgctcgat cgccactgca aggttatgca atgccgttct tggtttcggc atgaacgcga
      721 tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat tccattactc tgagggctac
      781 tgactgccgt cgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgag
      841 ccgtataagc cgaaccgtta tcggccactg gattggccgt tccggtcctt tatcgccata
      901 tcgctcctga catgacatcc tgcaccgttg ctttgtcata atctcccgtg cctgctgccc
      961 agtctatggc ttcacgatcg caatatggaa cgtaacgctg tcatcgggag aatcgtcaat
     1021 gcggcattcg gttaacgctt accaatatca acaccaaccg gatttgtgga gcctccagtc
     1081 tgaaggggct ttatgcgttc cgtataggat attatgatgc tggagttttg cgaagctcca
     1141 ctcctttccg aacatttaca cagatattgc ctaagaaaag tgaaaccata atatggttat
     1201 agtccctttc agtgcaaata cttagcttgt tgcacagaga aatagaagct gcataagctc
     1261 tttcttcaaa aaatgtaaat aaag
//
LOCUS       DAZPDE010000381         1283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPDE010000381 DAZPDE010000000
VERSION     DAZPDE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.388"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..1252
                     /gene="ospD3"
                     /locus_tag="ACHU0J_004276"
     CDS             <1..1252
                     /gene="ospD3"
                     /locus_tag="ACHU0J_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR1218658.1"
                     /translation="MALGLKIKETKNGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKK
                     LTVDNFLDEKHQKCYGLISDGMSIFVDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGL
                     TELIQKVTRVVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETS
                     GLNLDKTVELLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLL
                     TAYYYDEVHRQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVP
                     GLLLALNNGQADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETI
                     IAYGKILHTADLTPHQASKLLAAEGPNGVSGLIIAFQNRNFEAIKTYMEIIKNENITP
                     EEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          454 a          220 c          252 g          357 t
ORIGIN      
        1 tatggcatta ggcttgaaga ttaaagaaac taaaaatggg ggggattttg tcattcagct
       61 ttatgatccc aaccatacag caactcattt acgagcagag tttaacaaat ttaacttagc
      121 taaaataaaa aaactgactg tagataattt tcttgatgaa aaacatcaga aatgttatgg
      181 tcttatatcc gacggtatgt ctatatttgt ggacagacat actccaacaa gcatgtcctc
      241 cataatcaga tggcctgata atttacttca ccccaaagtt atttatcacg cgatgcgtat
      301 gggattgact gagctaatcc aaaaagtaac aagagtcgta caactatctg acctttcaga
      361 caatacgtta gaattacttt tggcagccaa aaatgacgat ggtttgtcag gattgctttt
      421 agctttacaa aatgggcatt cagatacaat cttagcatac ggagaactct tggaaacttc
      481 tggacttaac cttgataaaa cggtagaact actaactgcg gaaggaatgg gaggacgaat
      541 atcgggttta tcccaagcac ttcaaaatgg gcatgcagaa actatcaaaa catacggaag
      601 gcttctcaag aagagagcaa taaatatcga atacaataag ctgaaaaatt tgctgaccgc
      661 ttattattat gatgaagtac acagacagat acccggacta atgtttgctc ttcaaaatgg
      721 acatgcagat gctatacgcg catacggtga gctcattctt agcccccctc tcctcaactc
      781 agaggatatt gtaaatttgc tggcctcaag gagatatgac aatgttcccg gacttctgtt
      841 agcattgaat aatggacagg ctgatgcaat cttagcttat ggtgatatct tgaatgaggc
      901 aaaacttaac ttggataaaa aagcagagct gttagaagcg aaagattcta atggtttatc
      961 tggattgttt gtagccttgc ataatggatg tgtagaaaca attattgctt atgggaaaat
     1021 acttcacact gcagacctta ctccacatca ggcatcaaaa ttactggcag cagaaggccc
     1081 aaatggggta tctggattaa ttatagcttt tcaaaatagg aattttgagg caataaaaac
     1141 ttatatggaa ataataaaaa atgaaaatat tacacctgaa gaaatagcag aacacttgga
     1201 caaaaaaaat ggaagtgatt ttctagaaat tatgaagaat ataaaaagct gaatattatc
     1261 tcgtggtgat acactgaatt tga
//
LOCUS       DAZPDE010000382         1282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPDE010000382 DAZPDE010000000
VERSION     DAZPDE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.389"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1214)
                     /locus_tag="ACHU0J_004277"
     CDS             complement(<1..1214)
                     /locus_tag="ACHU0J_004277"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1218659.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGW"
BASE COUNT          302 a          340 c          339 g          301 t
ORIGIN      
        1 tgccagccgc gcagacgcag acgttcagag aggtcgtaca gggtgtatcc cggatcttca
       61 ccatctttca gtttgaagca aaccgccggg atgccttcgt ccgggcgacc cgtacagatg
      121 aactcatacg gcccctgttt ggcgatttca tccgccagat aagcggcaac ctggtaagag
      181 gcgttctgta ctttggtata gccttcacga ccgaggcgca ggaattcata gtactgtgca
      241 attacctgac ccgccgggcg ggagaagttg atggcaaaag taccaatttg accacccagg
      301 tagtcaacgt tgaacaccag ttcctgcggc agcgcttctt cgtcacgcca gataacccag
      361 ccgcagccca gcggagccag accgaattta tggcctgaag cactgatcga tttcacacgc
      421 ggcaggcgga agtcccagac gatatccggg gcgacgaacg gtgccaggaa gccaccgctg
      481 gcagcgtcga tgtgcatgtc gatgtcgata ccggtgtcgg cctggaattt atccagcgca
      541 tcgtgcagcg gttgtgggaa ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc
      601 acgccgatgg tgttttcgtc acaggcttca atcatgcgtt tcgggtccat aaacaactga
      661 ccggggcgca tagggatctc acgcagctcc acatcccagt agcgggcgaa tttatgccag
      721 cagatttgta ccggaccgca cactaggttt ggtttatccg ttggtttgcc tgcagcttcc
      781 atacgcttgc gccaacgcca tttcatcgcc atcccgccga gcatacaggc ctcggaagaa
      841 ccaatggtgt tggtgccaac ggcctgacca tttttcggcg caggcgcatg ccacagatcg
      901 gcaaccatat ttacgcaacg caggtcgatg gctgcggatt gcggatattc ttctttgtcg
      961 atccagtttt tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc
     1021 tggcagaaag tggctaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata
     1081 atctggaatg cgacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata
     1141 gtagaaatgg cctttgcgcc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac
     1201 agcttctggt ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt
     1261 atccgcaatg gaagcaaggc at
//
LOCUS       DAZPDE010000383         1277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPDE010000383 DAZPDE010000000
VERSION     DAZPDE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.390"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHU0J_004278"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHU0J_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR1218660.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          229 c          285 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actatctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAZPDE010000384         1275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPDE010000384 DAZPDE010000000
VERSION     DAZPDE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.391"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHU0J_004279"
     CDS             <1..730
                     /locus_tag="ACHU0J_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR1218661.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHU0J_004280"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHU0J_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          338 a          309 c          282 g          346 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
     1141 tctgaatcgc cacgggttta acagacacct cagagtcatt taagatggct taaagagagg
     1201 tgcccatgag cggtaagcgt tatcccgaag agtttaaaac tgaagcagtc aaacaggttg
     1261 ttgatcgcgg ttatt
//
LOCUS       DAZPDE010000385         1248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPDE010000385 DAZPDE010000000
VERSION     DAZPDE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.392"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..1183)
                     /locus_tag="ACHU0J_004281"
     CDS             complement(<1..1183)
                     /locus_tag="ACHU0J_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1218662.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          408 a          288 c          245 g          307 t
ORIGIN      
        1 atgcggctcc ggcagcttct ttaggaacaa gctccattga ataaagacca ataaacatca
       61 ccggaccata cactgccagc cccaacagaa taaggattgt atagtttagc cagagatatt
      121 cattgcctga tggcacgtac cagtacgcga ttaataaaat aatggtaagt gccagaaata
      181 aagtatttgt taatggtttt gagtttctga acagtctgac agacaggtat ccggcaagaa
      241 gcataccagc cacaccaccg atttcaaaaa cagaaccccc ccccattgat gccagttttc
      301 ccatatcctt accaccgaca tccagactgt cacttaaaaa cttaggagcc caggaaacaa
      361 taccgtatct cagaatataa acaaacgaac catagataca gcagtaaatg agtttattat
      421 ttcggatgat atgttttctg atgatatcag gaatggagag tctcggacct tgttcacggc
      481 ctttagccgg gtgtgaaacg acgttacctt tccattcttc aatgggagga agccccactg
      541 atacaggtct gtccacctga ataaagtaaa tcagcacact cattgccata cacagtactc
      601 ctgggaagat aaatgcggcc cgggcatgat ccagagcact gtcgccgaaa aaaccaaaac
      661 tgaaaccaat cagaacgggg gccagagctc ccccgacatt gtgcgataaa ttccacagtg
      721 tggcccaggt gatcctttcc cgatgttcat accagaacac cagtgttttg gcaccaggag
      781 gatatcccat accttggatc cagccgttaa gtaaatacat tgttgaaaat aacgtaacgg
      841 atgtgatgat cccgggaata tatcctatca gaaaatttac cagtccggac aggaataaac
      901 caagagacaa aaatactttt gaattacttc tgtcagaaat aagtccggaa acaaactttg
      961 atataccata ggataaaaaa ccggcacagg agataatgcc gatctcggtt gcagacatgt
     1021 gcagcacacc ggataacaca ctggtggtgt ttcggacaaa ataatacccc atatatccga
     1081 ggaaaatgct gataaagacc ctgtatcgca ggacgggata taatcgttca atctcttcag
     1141 agggtaatct ttcaatttcc ttaggttctg tgaataatga catatttaat ccttctgtaa
     1201 tactgataac caattgtcgc aaacctgttt acttatcatt atataatc
//
LOCUS       DAZPDE010000386         1219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPDE010000386 DAZPDE010000000
VERSION     DAZPDE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.393"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(3..485)
                     /locus_tag="ACHU0J_004282"
     CDS             complement(3..485)
                     /locus_tag="ACHU0J_004282"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1218663.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACHU0J_004283"
     CDS             complement(476..778)
                     /locus_tag="ACHU0J_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1218664.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..>1219
                     /locus_tag="ACHU0J_004284"
     CDS             1050..>1219
                     /locus_tag="ACHU0J_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218665.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYP"
BASE COUNT          364 a          307 c          252 g          296 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtaccc
//
LOCUS       DAZPDE010000387         1217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPDE010000387 DAZPDE010000000
VERSION     DAZPDE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.394"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHU0J_004285"
     CDS             complement(108..479)
                     /locus_tag="ACHU0J_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218666.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..1037)
                     /locus_tag="ACHU0J_004286"
     CDS             complement(540..1037)
                     /locus_tag="ACHU0J_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218667.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1066..1217)
                     /locus_tag="ACHU0J_004287"
                     /pseudo
     CDS             complement(1066..1217)
                     /locus_tag="ACHU0J_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT          286 a          316 c          290 g          325 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
     1081 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     1141 cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1201 tgtccaagat aagcctg
//
LOCUS       DAZPDE010000388         1189 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPDE010000388 DAZPDE010000000
VERSION     DAZPDE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.395"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(250..324)
                     /locus_tag="ACHU0J_004288"
     tRNA            complement(250..324)
                     /locus_tag="ACHU0J_004288"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Gly,seq:tcc)
     gene            complement(328..402)
                     /locus_tag="ACHU0J_004289"
     tRNA            complement(328..402)
                     /locus_tag="ACHU0J_004289"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(367..369),aa:Thr,seq:tgt)
     gene            complement(405..481)
                     /locus_tag="ACHU0J_004290"
     tRNA            complement(405..481)
                     /locus_tag="ACHU0J_004290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Arg,seq:tct)
     gene            complement(483..559)
                     /locus_tag="ACHU0J_004291"
     tRNA            complement(483..559)
                     /locus_tag="ACHU0J_004291"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Ile,seq:cat)
     gene            complement(749..>1189)
                     /locus_tag="ACHU0J_004292"
     CDS             complement(749..>1189)
                     /locus_tag="ACHU0J_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1218668.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          316 a          316 c          247 g          310 t
ORIGIN      
        1 ttcccggggt ttgcttagaa tattttgtaa gttgtccgaa gagatattta caacaccaga
       61 atgatgcatt atcagtcccc tgccagaggt tcactacgta acctgaccga caatgcacca
      121 ttctggtgct gaaaaaagag cactggaact gcaacaaaac aacatcacag aacaggagat
      181 ggtaaggagt cagacattcc cacacaatat tgtgtcagtg caaataacaa ccttcgtctc
      241 aaatctcgct ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      301 taatagccat tatacgatgc ccgcatatgg tgcgactacc ggaatcgaac tggtgaccta
      361 ctgattacaa gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg
      421 actcgaacct cgcaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg
      481 gttggtggcc cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctga
      541 ccaactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
      601 catctgatca ccagcacgtt taacgtcctg tgccgttttt caggtataaa aaaaaccgca
      661 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
      721 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      781 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      841 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      901 tgcatttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      961 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
     1021 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
     1081 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
     1141 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcc
//
LOCUS       DAZPDE010000389         1172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPDE010000389 DAZPDE010000000
VERSION     DAZPDE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.396"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHU0J_004293"
     CDS             complement(<1..666)
                     /locus_tag="ACHU0J_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218669.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQD"
BASE COUNT          331 a          178 c          226 g          437 t
ORIGIN      
        1 atcttgccct gtcatgttga aatcaataaa gtcgcatcca gcaagggctt ccatcctatt
       61 ttcattttta ctgtgctcaa tatacagctt cgccattttt aagtacaagc tttgaatgtc
      121 tcttatatca gttggtaagt cagctcgttg cccagcaaaa tttttattat cggaagaaac
      181 ggtttggaca ctgaatcttt cgggtattcc ttcttctacg agaattttag ttatgattgg
      241 attgttgggt tctggattaa cgacaacaat gatttgacat acagaatcct tatccttttc
      301 gtatctaatg ttatgaatct gattaagtat tgattgtgtc tgttgcgtaa gtggtgtcga
      361 acttatcgtt tcaatgttat taataagact tacaagttga accacaactt ttgagtagtc
      421 tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt tgttggagtt gttgttgcgc
      481 taaatcaaat tgttcagggt tgtatccttc tccagagaat gtggaattaa tgggttgttg
      541 ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc gttttaacat tatcggtatt
      601 tgttgtgcag ttataaatat cgccgtgata tgtatttatt atacctgggg taaagtcaat
      661 tgacataaca ttccttattc atcatctgtt ttatgctaat gtttttaaat tggataatta
      721 gaacaggaat ggattttctt ctacacacaa taaatataat gcgttatatc ttcctgatat
      781 aattaccttt aaattccctt tcatttccac ccggtaatat ttgtgtataa aaaaagggca
      841 gtgatagtat cgtaaaggaa aacgttaaca actatcaaaa aacgctcatt ttaaaaagtt
      901 attatgttct ccgatagtgt gtgaagtaat atttttgtga tgttttagat gtgtaataat
      961 gaaccaggtt aaagtgtatt taaaaatgtg ttgtattagc gactgaaatc tgccttttaa
     1021 aaaggaggag ggtactgaga caaagagtca ttgcttctcc gataaaatgt cagagaagca
     1081 tgattacgtg gttattgtct gttgatgact tgttgaccga gccgtcatca ttctcaatgc
     1141 attagttaaa tcatttgtga tttttaagga tt
//
LOCUS       DAZPDE010000390         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPDE010000390 DAZPDE010000000
VERSION     DAZPDE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.397"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..308
                     /locus_tag="ACHU0J_004294"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHU0J_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            341..742
                     /locus_tag="ACHU0J_004295"
                     /pseudo
     CDS             341..742
                     /locus_tag="ACHU0J_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          339 a          254 c          292 g          276 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccc ggtttaatct
      601 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      661 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      721 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      841 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      901 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      961 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
     1021 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
     1081 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
     1141 ccaactagcg ttactaaatg t
//
LOCUS       DAZPDE010000391         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPDE010000391 DAZPDE010000000
VERSION     DAZPDE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.398"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            116..>1160
                     /gene="cadB"
                     /locus_tag="ACHU0J_004296"
     CDS             116..>1160
                     /gene="cadB"
                     /locus_tag="ACHU0J_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1218670.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          256 c          298 g          360 t
ORIGIN      
        1 ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc agtttggtct ggtcaggaaa
       61 tagttataca tcatgacccg gactccaaat tcaaaaatga aattaggaga agagcatgag
      121 ttctgccaag aagatcgggc tatttgcctg taccggtgtt gttgccggta atatgatggg
      181 gagcggtatt gcattattac ctgcgaacct agcaagtatc ggtggtattg ctatctgggg
      241 ttggattatc tctattattg gtgcaatgtc gctggcgtat gtatatgccc gactggcaac
      301 aaaaaacccg caacaaggtg gcccaattgc ttatgccgga gaaatttccc ctgcatttgg
      361 ttttcagaca ggtgttcttt attaccatgc taactggatt ggtaacctgg cgattggtat
      421 taccgctgta tcttatcttt ccaccttctt cccagtatta aatgatcctg ttccggcggg
      481 tatcgcctgt attgctatcg tctgggtatt tacctttgta aatatgctcg gcggtacctg
      541 ggtaagccgt ttaaccacta ttggtctggt gctggttctt attcctgtgg tgatgactgc
      601 tattgttggc tggcattggt ttgatgcggc aacttatgca gctaactgga atactgcgga
      661 taccactgat ggtcatgcga tcattaaaag tattctgctc tgcctgtggg ccttcgtggg
      721 tgttgaatcc gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta ccgttccgct
      781 ggcaaccatg ctgggtactg gtttagcagg tattgtttat atcgctgcga ctcaggtgct
      841 ttccggtatg tatccgtctt ctgtaatggc ggcttccggt gctccgtttg caatcagtgc
      901 ttcaactatc ctcggtaact gggctgcgcc gctggtttct gcattcaccg cctttgcgtg
      961 cctgacttct ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa
     1021 cgacggtaac ttcccgaaag tttatggtga agtcgacagc aacggtattc cgaaaaaagg
     1081 tctgctgctg gctgcagtga aaatgactgc cctgatgatc ctcatcactc tgatgaactc
     1141 tgccggtggt aaagcatctg
//
LOCUS       DAZPDE010000392         1128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPDE010000392 DAZPDE010000000
VERSION     DAZPDE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.399"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>1128
                     /locus_tag="ACHU0J_004297"
     rRNA            <1..>1128
                     /locus_tag="ACHU0J_004297"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          330 a          220 c          332 g          246 t
ORIGIN      
        1 ggaccgaacc agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc
       61 aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgatgtta
      121 agtctcttgg aggtagagca ctgtttgggt gaggggtcca tctcggatta ccaatctcag
      181 ataaactccg aatgccaacg agatatcatc ggcagtcaga ctgcgagtgc taagatccgt
      241 agtcgaaagg gaaacagccc agaccaccag ctaaggtccc aaaataactg ttaagtggaa
      301 aaggatgtgg ggttgcacag acaactagga tgttagctta gaagcagcta ttcattcaaa
      361 gagtgcgtaa tagctcacta gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac
      421 agtttaccga agctgtggat gacacaaaag tgtcatggta ggagagcgtt ctatgtgtga
      481 agaaggtgta ccgtgaggag cgctggaacg catagaagtg agaatgccgg tatgagtagc
      541 gaaagacagg tgagaatcct gtccaccgta agactaaggt ttccagggga aggctcgtcc
      601 gccctgggtt agtcgggacc taaggagaga ccgaaaggtg tatccgatgg ccaacaggtt
      661 gatattcctg tactagagta tatagtgatg gagggacgca gtaggctaac taaaccggac
      721 gattggaaga gtccggctaa gcagtgaggt gtaagatgag tcaaatgctt atctttataa
      781 cattgagctg tgatggggag cgaattttag taacgaagtt agtgatgtca cactgccaag
      841 aaaagcttct agcgtttaat gatactctac ccgtaccgca aaccgacaca ggtagtcgag
      901 gcgagtagcc tcaggtgatc gagagaactc tcgttaagga actcggcaaa atgaccccgt
      961 aacttcggga gaaggggtgc tgacttaggt cagccgcagt gaataggccc aagcaactgt
     1021 ttatcaaaaa cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc
     1081 cggtgctgga aggttaagag gagggtttag cgcaagcgaa gatctgaa
//
LOCUS       DAZPDE010000393         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPDE010000393 DAZPDE010000000
VERSION     DAZPDE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.400"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHU0J_004298"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHU0J_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHU0J_004299"
     CDS             992..>1109
                     /locus_tag="ACHU0J_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218671.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZPDE010000394         1103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPDE010000394 DAZPDE010000000
VERSION     DAZPDE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.401"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            11..961
                     /gene="virK"
                     /locus_tag="ACHU0J_004300"
     CDS             11..961
                     /gene="virK"
                     /locus_tag="ACHU0J_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR1218672.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1026..>1103
                     /locus_tag="ACHU0J_004301"
     CDS             1026..>1103
                     /locus_tag="ACHU0J_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218673.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWF"
BASE COUNT          318 a          206 c          229 g          350 t
ORIGIN      
        1 aagtaacaat atgttttcta taagtaactt atcatttatc ggtttcctta aaaggattat
       61 tttttcctca gattcactac cggggaagtg ggaacacaga aaatttcggt tcatgtacat
      121 tttgcgatgt tctataaatc cggttgtcag tattcgatat tattatgaac tgcgttcctt
      181 gccgtgcatt gaggatattc tggcaataca ccccacgttg ccagcaagaa ttcatcgccc
      241 ttatttgcat aaaggggggc gcgcctggac ccgtggacaa tatattcttg agcattatcg
      301 ctttgtgcaa aatttgccag aaaaatattc taaatttttg tttccacaaa aatcggtatc
      361 attagtccaa ttcatcggga aagatggaga gaattttgat atccagtgtt ctcccagtgg
      421 ttttgaccgg gaaggagagt tgatgttgtc attgttttac aataaaacag ttattgcccg
      481 gttaacattt tcagtcattc tgactcaaaa tggacacatt gcttttattg gcggattaca
      541 gggagcccct aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg
      601 gattttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgcaatat
      661 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
      721 tcagaaacgc aaaacctttg ttgcggtcta tagtgatttc tgggagtctg tagcaggaaa
      781 aacctgtggt gactggtaca gattaccaac ccaggtgatt cgaaagccgc taagcgatat
      841 agccagtaaa aaacgctctg ggtaccgaaa acgatacgct ttgctggatt atatccatga
      901 aactactatc cgctctttgg atgcatatcc tgtacactca gaacatcaga atttaaatta
      961 aggtttgatg tattctctga ataaaacagt aatgataatt ttggtagagg cattcgcact
     1021 aaataatgaa aaaatataaa tctgagttta ttcctgaatt taagaaaaat tacctttccc
     1081 ctgtttactg gtctacatgg ttc
//
LOCUS       DAZPDE010000395         1103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPDE010000395 DAZPDE010000000
VERSION     DAZPDE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.402"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            20..355
                     /locus_tag="ACHU0J_004302"
     CDS             20..355
                     /locus_tag="ACHU0J_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1218674.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(636..744)
                     /locus_tag="ACHU0J_004303"
                     /pseudo
     CDS             complement(636..744)
                     /locus_tag="ACHU0J_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(803..1081)
                     /locus_tag="ACHU0J_004304"
     CDS             complement(803..1081)
                     /locus_tag="ACHU0J_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218675.1"
                     /translation="MNIDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLSGRD
                     VIILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
BASE COUNT          356 a          285 c          190 g          272 t
ORIGIN      
        1 gcataaacaa ggaaactaaa tgaagtggat tgtgattgat acagttatcc agccatcatg
       61 cggaatatct ttttcagtca tatggagtaa aataaaatta ataatctggt atcaatcgga
      121 tgctttctta cctcctgaaa gtatatttac actgactcac acaggcatca tgctcaataa
      181 caaagtgcta cctgtaacca tttacaacgt agtaccattc aataaaacat tctggaattt
      241 aatcaaaaac agccaggaat gccctacaaa tacagataac gtattgaatg aatgctttaa
      301 taaccgttgc actctgcaaa tatgccctta tggactaaaa caacaaagtc cataaggagt
      361 ttactcacat ctgacaaaat caatataaac agcccctccg gagaggggct ggagagtggc
      421 gctatgtgcc attccatggt gccgggtgcc tcccggtgaa ttcagtacca gcacctgaat
      481 ccgcgattat cccatatacc tactcgctga ttgcccctcc gcacaggggg attcaccatg
      541 ccagtttctt ttaacaaact ccccgcaaac cagacaacag tcaaccgcct gaattgtgaa
      601 gtatttaaaa atttctcccg ctaactgata cccggctaac agtctggcgt tttctttttc
      661 agcaacggga aagcaacaac caccacaccc gccaccagca caccgtcagc cggcactgac
      721 attatccggc tgctgcaatg ccattaacaa aaacagtaag caatcacttt ttaccgtaac
      781 aggtgataat ccagatatgt atctacccca gatgagtaat ccgaagttca tccataccac
      841 aggtcctggc tattctgttg tactcctgaa caagagcaaa taattctgaa ttagcaacca
      901 tgaactcatc gcaaaccctc tgtatagcat cactattcag aataataacg tctcttcccg
      961 aaagacgatc aggagtacag aacaaaactg tcaaacggct gaaggccttt gctcgtgctg
     1021 cattgactat atcaatacgc tgcctaagga tgaaacaccc cgacgcctca tcaatattca
     1081 ctctacccac accatatgaa tga
//
LOCUS       DAZPDE010000396         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPDE010000396 DAZPDE010000000
VERSION     DAZPDE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.403"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHU0J_004305"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHU0J_004305"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1218676.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHU0J_004306"
     tRNA            complement(986..1062)
                     /locus_tag="ACHU0J_004306"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZPDE010000397         1086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPDE010000397 DAZPDE010000000
VERSION     DAZPDE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.404"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(25..987)
                     /locus_tag="ACHU0J_004307"
     CDS             complement(25..987)
                     /locus_tag="ACHU0J_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1218677.1"
                     /translation="MFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRGY
                     LLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFGW
                     KVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVS
                     CCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYG
                     FIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALII
                     GSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            complement(1012..>1086)
                     /locus_tag="ACHU0J_004308"
     CDS             complement(1012..>1086)
                     /locus_tag="ACHU0J_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1218678.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          321 a          258 c          236 g          271 t
ORIGIN      
        1 ttatagttta ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat
       61 agccatacct aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt
      121 aagtaatatc acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc
      181 tcccagaccc attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg
      241 gatatacagc ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc
      301 tgcatgagcg gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac
      361 tcctaacaaa agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag
      421 ttttatggga ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc
      481 aatggcttta gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg
      541 cgaagttttt gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg
      601 ttcaaacccc agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat
      661 cgcgtacaac aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg
      721 attaagtaat ggtgaaacaa ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga
      781 caacaatcca cgtaacatgg gtattgtcga gcaactacag aacggggtaa cggctcccaa
      841 cagagcagcc aggagatagc ctctcccttt tctggccccc atcatctgct ggattttatg
      901 gtctggcacc ttttgtctta tcaggctgac accggcacta atcactataa acagcaacga
      961 aagctcgacg gcgagaaaca caaacatctc tgcggcggta atgactccaa cttactgata
     1021 gtgttttatg ttcagataat gcccgatgac cttgtcatgc agctccaccg attttgagaa
     1081 cgacag
//
LOCUS       DAZPDE010000398         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPDE010000398 DAZPDE010000000
VERSION     DAZPDE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.405"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHU0J_004309"
     CDS             complement(<1..104)
                     /locus_tag="ACHU0J_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR1218679.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHU0J_004310"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHU0J_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1218680.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          296 a          245 c          188 g          341 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc
//
LOCUS       DAZPDE010000399         1069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPDE010000399 DAZPDE010000000
VERSION     DAZPDE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.406"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..375)
                     /locus_tag="ACHU0J_004311"
                     /pseudo
     CDS             complement(1..375)
                     /locus_tag="ACHU0J_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930142.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(326..>1069)
                     /locus_tag="ACHU0J_004312"
     CDS             complement(326..>1069)
                     /locus_tag="ACHU0J_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1218681.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGG
                     LIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPGTPTGTYIVSPQFFITGCSEH
                     SLPGSYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          235 a          298 c          268 g          268 t
ORIGIN      
        1 tcggggccag cattccagat acgaccgttc cattcaaacg tgaacggctt cgcctcctgt
       61 tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc tggccgcttc cagcatttct
      121 ggtgtcacag tgaatggggc tatctcaccc catttgccac tttgcagttc ctgccagatt
      181 tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt aggggactgc ctggtccctg
      241 tcgtcaaaaa aaaacgtcac agtctactgc gccactttcg gtataacggg gattaatgat
      301 ttttttaatt tccacggtgc attcctcacg atgtgcgaat aaaaagcccg ggcattgcgc
      361 cagagacatg tgcatccggc accccggaca gggcgcaata tgaccccggt aatgaatgct
      421 ctgaacatcc cgtaatgaaa aattgtgggg atactatata cgttccggtg ggagtaccgg
      481 gcactgaaat ccccaccggt cccagtcgtg agcctctgta tgactgcccc ctgacaagtc
      541 tgatgacttt atcaccgtca gcttctccct ggtacgcagc aataatcagc ccgccaatgt
      601 cagggtctcc ccatctgttg cggacagagc tcgccacgat tctgtaaata atatcttctg
      661 tggttatatt tattttcacc cagccagtca gtctggatat gggccagtag cagtagcggg
      721 tgtgataaat ggggccgtta atgccgtaaa aagtaaggga tttggctctg taccgcggtt
      781 ctgttgtctc agggcgtgca tcagtccacc ggatgctgag caccccttca aaccgtgtgt
      841 cgggtatgat gatgccgtag gggccagcaa cggaatattc acctggcagc gcattcctta
      901 cccaggccag gaaatcactc ttagtgtcaa aacggataac atcttcaggc agaaaagcac
      961 acccaaagcc gaatgcgccg ggtatcgcca gacggccttt tgtccggtcg taaatgtcgc
     1021 tctgtgcttc catcgtggcc gcacttttca gccccagatt atcccggga
//
LOCUS       DAZPDE010000400         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPDE010000400 DAZPDE010000000
VERSION     DAZPDE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.407"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHU0J_004313"
     CDS             <1..560
                     /locus_tag="ACHU0J_004313"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR1218682.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHU0J_004314"
     CDS             787..>1068
                     /locus_tag="ACHU0J_004314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR1218683.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZPDE010000401         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPDE010000401 DAZPDE010000000
VERSION     DAZPDE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.408"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(226..651)
                     /locus_tag="ACHU0J_004315"
     CDS             complement(226..651)
                     /locus_tag="ACHU0J_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1218684.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(648..1031)
                     /locus_tag="ACHU0J_004316"
     CDS             complement(648..1031)
                     /locus_tag="ACHU0J_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR1218685.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          307 c          265 g          243 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcgat aaaaacatcg ccatatcctc
      421 aatgtaaggg tgggacttct ccggattcag caccacacag tccgccttct gttgctcact
      481 cagggcatgt aaatcatgct caaaccacgc cccctgagca tctgtctgca gaatcaaccg
      541 gccatggcag gaaaggcaga aacactgccg gatatttctg ctgagcgcag agtcggcact
      601 gataatgtgc tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc
      661 ccctcctcac tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg
      721 tggtgacatt gcctgcagtg ccagctggct ttacgtacta cgggtaaggt atccgggacg
      781 aattgctgca gacgtttaat caaccgtatt tctctgcgtt ccggcctgac ataagggcac
      841 tcatgaccgt gctccgtcag cccttcgtca gcgtgttcaa accagggccg ttcagtgtcg
      901 tattgcgggt ggtatctgag tgcactgccg caaaggtggc aggtgtagct atcgtaaggt
      961 gcagtctgtg cggtacgggc accggtcaga cgtccgttgc catcaagtgc gagaaaagat
     1021 tttgcgtaca tagtatatgt tcctcagccg ccagacgaca cgcaggca
//
LOCUS       DAZPDE010000402         1065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPDE010000402 DAZPDE010000000
VERSION     DAZPDE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.409"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHU0J_004317"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACHU0J_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR1218686.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          299 a          322 c          249 g          195 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accct
//
LOCUS       DAZPDE010000403         1064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPDE010000403 DAZPDE010000000
VERSION     DAZPDE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.410"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..227)
                     /locus_tag="ACHU0J_004318"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="ACHU0J_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            211..469
                     /locus_tag="ACHU0J_004319"
                     /pseudo
     CDS             211..469
                     /locus_tag="ACHU0J_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(578..>1064)
                     /locus_tag="ACHU0J_004320"
     CDS             complement(578..>1064)
                     /locus_tag="ACHU0J_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218687.1"
                     /translation="AVLSGKINELNACHKVAIFLAEKDNEITKKDKAKIIDTLTENYS
                     IEFQQLMNISERTLNSSLYITPGESGFVSFVNREGKICHTAYVKSSDNSMTYYHANGS
                     SIDKYITDMCGLICMRHIESTGIIFYMLDEKVLSAIAEFMNEKGWRAAFCSAKNLYKC
                     V"
BASE COUNT          302 a          247 c          189 g          326 t
ORIGIN      
        1 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
       61 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
      121 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
      181 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
      241 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
      301 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
      361 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
      421 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
      481 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
      541 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
      601 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
      661 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
      721 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
      781 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
      841 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
      901 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
      961 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     1021 tatgacatgc attcagctcg ttaattttac ctgataaaac tgct
//
LOCUS       DAZPDE010000404         1053 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPDE010000404 DAZPDE010000000
VERSION     DAZPDE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.411"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..590
                     /locus_tag="ACHU0J_004321"
                     /pseudo
     CDS             1..590
                     /locus_tag="ACHU0J_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          304 a          240 c          239 g          270 t
ORIGIN      
        1 tggtgtatat ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag
       61 ataacggaat tatcgtcggt cgtgacagac tggcaggtct tcgtaaggag ctgaggctgc
      121 actgtaagca gaaacgcaag ttcagggcga ccacaaactc tgaccacaat ctgccggtta
      181 cgccgaatct gctgaatcag aactttacgc ccacagcacc taatcaggtc tgggtcgcag
      241 atataacgta tgttgccacc cgggaaggat ggctgtatct cgctggcgtc aaagatgttt
      301 atacgtgcga aattgtcggt tacgccatgg gagagcgcat gacgaaagag ttgacgggta
      361 aagccctgtt tatggcgctc aggagccagc acccacctgc cgggctaatc caccactccg
      421 ctcgaggttc acagtactgc gcatacgatt accgggtcat acaggagcag tctggtctga
      481 aaacatcaat gtcgcgtaaa ggtaactgtt acgacaacgc tccgatggaa atcttctggg
      541 gaacgctgaa aaatgaaagc ctgagccact atcgttttaa gagccgggat atttcgtcag
      601 catacgggaa aactgattga ggtcggcctc agttttgagg cctttagcca gttcagccgc
      661 aagggcttta agcttctttt cgtccataat ttacctgtct ccgttgttgg agtaaacata
      721 tcaaaaacag gcaattacac aatttaaatt acagtctcaa aacaaaccca acggtcaata
      781 tagtttacca gcaagcttac tagcaaagtg ctaatcattt ggtatctttc gtgctattct
      841 ccttttacgt aagtacttaa ggggaacgta tttactactt ttaaagaaag ctccgttact
      901 atctgaaatg caatgagata ataaacgatt actctcggtt agtatctcat gatcatttac
      961 agactttgca taatcgcgaa tactatataa cagagtttta acattacagg atgaatagtc
     1021 tctcttatca actcctaata aaagaaggat tat
//
LOCUS       DAZPDE010000405         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPDE010000405 DAZPDE010000000
VERSION     DAZPDE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.412"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..131
                     /gene="yecU"
                     /locus_tag="ACHU0J_004322"
     CDS             <1..131
                     /gene="yecU"
                     /locus_tag="ACHU0J_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1218688.1"
                     /translation="ADITLKKQPLLFLAKLMVYSAALTFFTTNFHCNMTRKINEYA"
     gene            118..342
                     /gene="yecF"
                     /locus_tag="ACHU0J_004323"
     CDS             118..342
                     /gene="yecF"
                     /locus_tag="ACHU0J_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1218689.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(375..995)
                     /gene="sdiA"
                     /locus_tag="ACHU0J_004324"
                     /pseudo
     CDS             complement(375..995)
                     /gene="sdiA"
                     /locus_tag="ACHU0J_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT          300 a          258 c          223 g          268 t
ORIGIN      
        1 ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact
       61 ccgccgcctt aacatttttc accacaaatt ttcattgcaa catgacgagg aaaataaatg
      121 agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc
      181 ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg
      241 atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt
      301 tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag
      361 cgtgcctttc agccggttat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc
      421 gccgtccatt tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact
      481 atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt
      541 aattgcagtt catcactaag aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa
      601 aagcccagcg cccgatttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt
      661 gcgtgcgcgg cttcccataa tggctgtgct tcgctgaata agtcatcatt ccacattaaa
      721 tggccctgac taaagttttc agggttcagc acaggatcaa ttgcgagaaa attttttgcc
      781 tgataataac taacccacga ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg
      841 aatggtaccg ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga
      901 agctctattt catggtaggc ctcttctgcg gcctccatcc tctgaaaacg caacagcatc
      961 gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt
     1021 gagtatcatt tataatgata attcaaact
//
LOCUS       DAZPDE010000406         1041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPDE010000406 DAZPDE010000000
VERSION     DAZPDE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.413"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..165
                     /locus_tag="ACHU0J_004325"
                     /pseudo
     CDS             1..165
                     /locus_tag="ACHU0J_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(182..872)
                     /locus_tag="ACHU0J_004326"
                     /pseudo
     CDS             complement(182..872)
                     /locus_tag="ACHU0J_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            951..1041
                     /locus_tag="ACHU0J_004327"
                     /pseudo
     CDS             951..1041
                     /locus_tag="ACHU0J_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          269 a          240 c          273 g          259 t
ORIGIN      
        1 gcagaaaaac ggcttgcggt caggcaggag aaatgtgtgc cgcgaatgaa gtccttgtat
       61 gactggataa agctccagag gaaaactttg tcaaaccatg ctgaaatggt aaaggcgttc
      121 aactatatcc tgaatcactg gaatgcactg aatgagtttt gccgggtagt acttccaatc
      181 attagaatat gtgcttttca atgaaggaac cgatgacttt ttcgtggatt tccacggaac
      241 gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggggcggagg gtcaggttgt
      301 tacgttcata cgttgcgtga agattttgcc ggtcaggtgt ttctcctccg gcacttctct
      361 ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag tcaacagggc
      421 taacagttcc cgacaggtct catcattccg tggcccgcaa gtgtaagcca acacaccgcc
      481 agttttagtg ttgtacgcat accagggcca gtgttgttgc gccttgctgc caacgaagcg
      541 ccactgctca tcaagttcac agataagggc aacatcagcg tgagcgactg gcgcagatgt
      601 tatccgccgg ggcgagagct ttttaatgtc cgtatgacgg tattgatgcc gattttcagt
      661 gtcctggcgg tatcacggcc ccctgcgccg ttatgtgcca tttcaacgat ctgctcctta
      721 acgccgagtt ttctggcttc ataagagtaa gtgagctgga aaacacgttt acatgagcga
      781 caacggaaac gctcatgccg ggaacaacta cggccatgtc gataaacttc atcggaatta
      841 cagcgcggac aatgaacagt aacggtagtc atggaaaacc tcaaatacca gtagattacc
      901 agaaacttct atcgattgaa gacattacct tttgccgcaa taggtggggt aacattggtg
      961 aaaacgcatt acgccgggtg gcagttggaa aaaaattatc ttttttcggc tctgatcgtg
     1021 gtggagaaaa tgcggcaata a
//
LOCUS       DAZPDE010000407         1036 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPDE010000407 DAZPDE010000000
VERSION     DAZPDE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.414"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..405)
                     /gene="mxiG"
                     /locus_tag="ACHU0J_004328"
     CDS             complement(<1..405)
                     /gene="mxiG"
                     /locus_tag="ACHU0J_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR1218690.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDVIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIV"
     gene            complement(414..872)
                     /gene="ipgF"
                     /locus_tag="ACHU0J_004329"
     CDS             complement(414..872)
                     /gene="ipgF"
                     /locus_tag="ACHU0J_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR1218691.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(872..>1036)
                     /gene="ipgE"
                     /locus_tag="ACHU0J_004330"
     CDS             complement(872..>1036)
                     /gene="ipgE"
                     /locus_tag="ACHU0J_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR1218692.1"
                     /translation="EKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYYISRVRWLK
                     DEFARRMKGY"
BASE COUNT          315 a          235 c          132 g          354 t
ORIGIN      
        1 aacaataaca gcaaagaaaa aaaacaccga gtggttctta tacattccgt ttagtatatg
       61 gcctctcgat ttatcctccc tcatattttt aaggtgaaaa gaaatcacgt catattcaaa
      121 tgaattaaat tgtgaattga tatcaatttg ctcacctttt tcatcactaa attgacagat
      181 attatctttg cttatggtca aataaattat accatccgat ttactatctg ttacaggaat
      241 aatattttct gggaaattgt cgtttccaaa ctcaagtgtt tctatcgtcc gtccgagaac
      301 aattaagtta ttcccataat ataacggaaa ctcatccccg acaccgtttg tgagtttcac
      361 taataacctg aatggtgcaa gatttgagtt ctttgcctca gacataaatc ctcttatatc
      421 cttcgattat tctgcttgct ctctgctgct atccgcaaat agcgtctata gatatcctct
      481 gcgtacttta atctttcttt tttctttttc ggagacgttc ctgcattata ggctccaaca
      541 gcctcccacc ctcttccata catcatcata aactcattta ataactttgc tgcataatgt
      601 acagaaagac atggatgact aattagcatt tcctcagaat atcccatttc tctaagtctt
      661 ttagaatgaa agtcatttat ttgcattata ccataatctt tacttccatt gttattaaca
      721 ttgactgcag atttattaaa tccggattct ttttccgcaa tcgcttttaa caaacttgat
      781 ggaatattgt acctttcacc agccttatcc caacaatcag ctctccctaa atatgggata
      841 aaacacagca aaataaatac gaaacgggac attaataccc cttcattctt cgcgcaaatt
      901 catccttcag ccaacgcaca cgcgaaatat aatattctgt attaacatat acatcctcga
      961 attcatttat cccggtaaga tctaatcgcg caattagatt tccgccttca tcatcagtag
     1021 ctaagcatat cttttc
//
LOCUS       DAZPDE010000408         1033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPDE010000408 DAZPDE010000000
VERSION     DAZPDE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.415"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            249..547
                     /locus_tag="ACHU0J_004331"
                     /pseudo
     CDS             249..547
                     /locus_tag="ACHU0J_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            629..766
                     /locus_tag="ACHU0J_004332"
                     /pseudo
     CDS             629..766
                     /locus_tag="ACHU0J_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          328 a          186 c          221 g          298 t
ORIGIN      
        1 aaaaacaata ctattttagg tgatgtatta aattagtaga atatttattc ttctataaaa
       61 gcactacccc aagaggttaa tcaggtcatc catgattatt tatcatatac taactatgtg
      121 ggatttcaac gtgaaaactt attttcacta taatcttcag gattcgaaga tcataggagc
      181 ctgtacataa gtttgtataa ttctctgatg ttgaactatt taatttgata tcaaatgaag
      241 gtgaatttat gaatgaaaaa cagttgacat ctaataagtt gcgggcttta gctaatgaac
      301 tagccaaaag tctcaaaaat cctgaagatc tcagtcagtt tgattggatg ctgaaaatga
      361 aaccgtactc aatgctaata tgactcatca tcttgggtgc gagaaaaatc agcttagatc
      421 tggcagcaat tcccgcaatg gttgcttaac gaagattatc actactggag atgaacctct
      481 ggaaatccgt actctgcgtg atcgtaatgg cactttcgaa ccgcaacagc tgaagaaaaa
      541 ccagccctag attactaaat ttgtcgggcg atggattaac tggttcaggc tgtctggtgc
      601 tgaaggcctg aagtcactgc tgtcagtgat gtggccggct cttcatgaga caataacccg
      661 taatcatcag tgccgttcaa tatggccact gttgaaaaaa gttcgtcact ttatggagac
      721 ggttagccca cttcccggag aaaaacatag tctggacaaa gtgtagcggt attaggctca
      781 catatttaga tgtagataag ccggagctgg cagttaaatc taggcagcat cacagaaatg
      841 gacaacgccc cttaaggact ggcgtatggc aatgagctgc tttattatca agtttggtga
      901 tcgccttgac ggtcacttct gagtgaaggc gcttgcacag aatggtaaac ggactcgatt
      961 attgaatctt agggataaga tatatctacg atatgtcaat atacgaaatc gaaagaattt
     1021 attggtgaat ata
//
LOCUS       DAZPDE010000409         1030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPDE010000409 DAZPDE010000000
VERSION     DAZPDE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.416"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            236..429
                     /locus_tag="ACHU0J_004333"
                     /pseudo
     CDS             236..429
                     /locus_tag="ACHU0J_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            572..793
                     /locus_tag="ACHU0J_004334"
                     /pseudo
     CDS             572..793
                     /locus_tag="ACHU0J_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(814..876)
                     /locus_tag="ACHU0J_004335"
                     /pseudo
     CDS             complement(814..876)
                     /locus_tag="ACHU0J_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          304 a          250 c          238 g          238 t
ORIGIN      
        1 tctccaggat tcccggggcg gttcaatcaa agcgcagaac ttctgcgtct tcttcgtcaa
       61 aaaaatcatc ttcgtgaagc ttcacgacat agcggggaag tttgcttctt tgagaggcgg
      121 gtttacgttt acgggattta gctgaacggg ccatataacc acctgaaaga caatgacatt
      181 gcctgttttt ataacggtaa ttgcagacca tgacaaaccg cagccgtcag gctgcctact
      241 cgagtcacaa ggctctttgt gtccgatatg ccggcagcgc attacccatg aaactggctg
      301 gaacgtacac cataaggtga agaaagttat gggggcggtg aagaattatc taaccttgtc
      361 ttgttacatc caaattgcca tcgacagtta cacagttgtg aagccggctc tctcaaaaag
      421 ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat gcaccgttct taggggaggg
      481 cagtccagca atgaactgtc cttacccgac aaacatccaa aacgaacgga acgtgctgga
      541 atccgcatat aacccgttgt aaagtgagga aatggacacc tcacttgctc atgagaacgc
      601 ccgcctgcgg gcactgttgc agacgcaaca ggacactatc caccagatgg ctgaatacaa
      661 ccgcctgctc tcacagcgga tggcggctta tgcttccgaa atcaaccgac tgaaggcgct
      721 ggttgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc gcgcaaaaac
      781 cgaacggcag attccatttt cacgggcgat ctacgccaca caggctctgg gttgcagtga
      841 ctgctccatt aaggcgattt tgaactcata agggaagtaa caaaaaaact gcaaaagtac
      901 aataattata tatggccaaa ctaacctcta ttaaaaaaca tcagtttttc acacttagtt
      961 gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca ctaactggaa
     1021 gcactccata
//
LOCUS       DAZPDE010000410         1019 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPDE010000410 DAZPDE010000000
VERSION     DAZPDE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.417"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>1019
                     /locus_tag="ACHU0J_004336"
     CDS             <1..>1019
                     /locus_tag="ACHU0J_004336"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR1218693.1"
                     /translation="DFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFKVLN
                     SCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTA
                     ELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQ
                     EKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLY
                     QVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLC
                     SADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPPYNE
                     QQAIF"
BASE COUNT          339 a          195 c          205 g          280 t
ORIGIN      
        1 gattttgttg tcggcgctga cggtgtgaag attttatcac ctatattaat ttgtgagcgt
       61 ttcttttttt ggcagttacg atcatttaaa ttagatgtaa gaggatacgc gagacatttt
      121 aaagtattaa atagctgctt atttgcatta ccccccattg cagaacaaga gcgaatagtt
      181 gaaaaagtta gctctttgat gtccctctgc gaccaactag aacagcaatc cctgaccagt
      241 ctggacgcac atcagcaact ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac
      301 accgcggaac tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc
      361 accgaagcca gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa
      421 cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac tgctcaaacg tattgcgcag
      481 gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac aaaaaccact gccgccaatt
      541 agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg aatggtgtcg tattggtaat
      601 atagttaata ttaaatctga attagtatca ccaaaagatt atttaaattt atatcaagta
      661 gcacctgata ttattgaaaa gggcactggg agagtgatat caaagagaac agtgaaagaa
      721 tcaggagtca aagggccaaa tagtcgcttt tataaaggac aaattgttta ttctaaaatc
      781 cgcccgtcat tatccaaagt ttttttagca gagtataatg gtctttgcag tgctgatatg
      841 tatccattag actgttacat taatccaaat tacttattga aatatatcct ttccattcca
      901 tttcttatgc aagtcaaaaa agcagagaac agaataaaaa tgccaaaact taattctgac
      961 agtttttaca atattattgt tgcgattccc ccctataatg aacaacaagc aatatttga
//
LOCUS       DAZPDE010000411         1018 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPDE010000411 DAZPDE010000000
VERSION     DAZPDE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.418"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..758)
                     /locus_tag="ACHU0J_004337"
     CDS             complement(<1..758)
                     /locus_tag="ACHU0J_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR1218694.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREV"
     gene            complement(788..>1018)
                     /locus_tag="ACHU0J_004338"
     CDS             complement(788..>1018)
                     /locus_tag="ACHU0J_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFR1218695.1"
                     /translation="FNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYG
                     LLGAAISWSIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          338 a          193 c          149 g          338 t
ORIGIN      
        1 acttccctaa tagagagtaa aagaggcaca tgattttttc taagcaaaag aaatgcattt
       61 cttactcgat aataacgacg tactggcgag tgaattggta tgttaatccc acagattttt
      121 actgacttat ccccaatagc atgaaccatt tctatatcag gaatgatatg aactaatatg
      181 ccatagttta agcatctaag acaccattct gtatcgacat aatcaataaa aagttcctca
      241 tccatcattc caacaatctc aaaaacttct tttgaaacca tggttccgga tgagattgtt
      301 actgaacttt taaacggtgt ctgtccttca gtgacatgaa ttttctctcg aagaccattt
      361 tttttgatat tacaaatagg ataataatgt gacttatctc tatcaataaa aactgggcca
      421 gctatttttg cattttcatt atccatatag caggaaagtt ttttgataaa ctcattgcga
      481 atacggctat cttgatcaaa aaaaattatc ttctctgccc ctttttcaag agaatatcga
      541 atcccaatat tttgtgcttt tgctatacct tcattcctac caagacaaat taaatgaact
      601 ttttcaatgt ctgctaattt atctttcaag gtgaaattag tcctattatc gactactaca
      661 acgtaacaat tttgctcctt tagttcagtg atcagtgcat tcagattttt aagttcggga
      721 ttatatgtca caatgacagc gtaaatatta ctattcatta tccaagtgac tcagtaattg
      781 gttaatttta gttttcatat acagaacaca ttctagtata aagccattgt aataacagag
      841 catctaaaat tacacgtatg ctccaggata ttgcggcacc cagtaaccca tatttttcga
      901 ccataaaata caaaagggct aaataaggaa ccaattcagc acaatgaatc aaagcagtaa
      961 ttttagattt tcctaatgcc tggattgcag agaatggtat cattgccagc gcattgaa
//
LOCUS       DAZPDE010000412         1004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPDE010000412 DAZPDE010000000
VERSION     DAZPDE010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.419"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            70..>1004
                     /locus_tag="ACHU0J_004339"
     CDS             70..>1004
                     /locus_tag="ACHU0J_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1239 family transposase"
                     /protein_id="HFR1218696.1"
                     /translation="MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLLGKAPQTI
                     HNEIKRGLVRQQVRKGKFEAVYSAQHAQLVYQENRKRSVRPIRQRKQDREAIEHYMRQ
                     NYSPEMIVKAKGVQVPVSTIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGK
                     SIEQRPDSINQRLEAGHYEIDTVILTRAKNQCLLTLTDRKTRHQLIRLIPDKSAQAVN
                     KALTGILKDYTVNSITADNGTEFSRLSDVFPASDIYYAHPYASWERGTNENHNRLIRR
                     CLPKGTKTTTPKEVAAIEHWINHYPKRLFNYKSPV"
BASE COUNT          335 a          225 c          206 g          238 t
ORIGIN      
        1 aaggtatgaa aaaagcacct ctaagaggta tacttgtagt tcaccacaaa cacaaggaaa
       61 ctcaaaaaga tgcaagatta ttatacacca aaaggcaaac aattgacaat ccatgagcgt
      121 cggcaaattc aacgctggaa gctggaaggt cgctctaatc gtgaaattgc tcgcttacta
      181 ggtaaggccc cccagaccat tcataatgaa atcaagcgag gattggttag gcaacaggtt
      241 cgtaaaggaa agtttgaagc cgtttattct gctcaacatg ctcagttggt ctatcaagaa
      301 aatcgcaagc gttctgttag acccattaga cagcgtaagc aagataggga ggctattgaa
      361 cactacatgc gtcagaacta ttcccctgag atgatagtta aagctaaggg agttcaagtc
      421 cctgtatcta ccatctatta ctggattcat catgggaaat taagtcttgg taaggaggct
      481 atgctttatc cgagaaaggc taagcaggct agaaaacatg ccagtcctta ttttaaaccc
      541 gcaggaaaga gtattgaaca acgcccagat agcattaacc aacgtcttga agctgggcat
      601 tatgagattg atacagtcat tctgacgagg gctaaaaatc aatgcttact caccctaacg
      661 gacaggaaaa cacgtcatca gctgattcga ttaataccgg ataaatctgc tcaagctgtc
      721 aataaagctc ttacggggat tctaaaagac tacaccgtca actctatcac ggcagacaac
      781 gggacagaat tttctcgctt atcagatgtc tttccagcaa gtgacatcta ctatgcccat
      841 ccctatgcgt catgggagag aggcactaac gaaaaccata atcgcctgat tcgcagatgt
      901 ctccctaaag gtaccaaaac aacgacccct aaagaagtcg ctgctattga acattggatc
      961 aatcattatc ctaaacgact cttcaactac aagtcgccag taga
//
LOCUS       DAZPDE010000413          980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPDE010000413 DAZPDE010000000
VERSION     DAZPDE010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.420"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(22..654)
                     /gene="mxiE"
                     /locus_tag="ACHU0J_004340"
     CDS             complement(22..654)
                     /gene="mxiE"
                     /locus_tag="ACHU0J_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR1218697.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(784..>980)
                     /gene="mxiM"
                     /locus_tag="ACHU0J_004341"
     CDS             complement(784..>980)
                     /gene="mxiM"
                     /locus_tag="ACHU0J_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR1218698.1"
                     /translation="NAGKFMGNFNVKEVDGCFMDAQKIAIDKLFSMLKDGVVLKGNKI
                     NDTILIEKDGEVKLKLIRGI"
BASE COUNT          319 a          194 c          124 g          343 t
ORIGIN      
        1 aagagagtca aagatttaat attaaatttt ttcatttatt tttttcacta aaaaagtaat
       61 attcgataat tctcttgcag aaaagcctag tcgtgttttt atctcatttg aaaaatggga
      121 tgttgatgca tacccattat tcatggcagc tgaagtgatt gtttgattgt gcaaaaatac
      181 atctaataaa ccatttacta accgccaagt gttcagttgt tctttgactt ttgcccctaa
      241 agcttttcta cataaagatc taaaataagc ctccgacaca ccataatgct ctgtcagact
      301 ctttattctt acctcctttt ctttctcaat agttctcaga agataaagaa ccaaatgata
      361 tgactcagtc attcgtatca tagccagtgc attacttaca ttctggtcag atgaaaagat
      421 gaatcttaac attaacttat caagcatcga ttcggtatta caactatccc gaagatcatt
      481 ctcatgatag aaatgaatac atttttcagc atggctatgc tctactagag caccactgaa
      541 atagtcccaa aaagcaagaa attttgcaca actattaatg ttaaagcgat aatgatagtt
      601 cacattatca ctgaatttta tattttgatt tcggacaaaa aaaaaccctt ccatgctgga
      661 gccagctcaa ttatgttacg ttctttatct tttacaagct caacgttaac tggttcatgt
      721 ccactagagt aaatgtaaaa catattactt gtatttagac ctttatattt actcacaata
      781 caattatatc cctcgaatta attttaattt aacttcccca tccttctcta taaggatggt
      841 atcatttatc ttattacctt ttaaaacaac cccgtctttc agcatagaaa atagtttatc
      901 tatagctatc ttttgtgcat ccatgaagca cccatctact tccttaacat taaagttgcc
      961 catgaattta cctgcattaa
//
LOCUS       DAZPDE010000414          970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPDE010000414 DAZPDE010000000
VERSION     DAZPDE010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.421"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHU0J_004342"
     CDS             <1..167
                     /locus_tag="ACHU0J_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR1218699.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHU0J_004343"
     CDS             152..790
                     /locus_tag="ACHU0J_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR1218700.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
BASE COUNT          260 a          189 c          287 g          234 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca
//
LOCUS       DAZPDE010000415          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPDE010000415 DAZPDE010000000
VERSION     DAZPDE010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.422"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            144..641
                     /locus_tag="ACHU0J_004344"
                     /pseudo
     CDS             144..641
                     /locus_tag="ACHU0J_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="ACHU0J_004345"
                     /pseudo
     CDS             682..873
                     /locus_tag="ACHU0J_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAZPDE010000416          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPDE010000416 DAZPDE010000000
VERSION     DAZPDE010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.423"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(55..243)
                     /locus_tag="ACHU0J_004346"
     CDS             complement(55..243)
                     /locus_tag="ACHU0J_004346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218701.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHU0J_004347"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHU0J_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAZPDE010000417          928 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPDE010000417 DAZPDE010000000
VERSION     DAZPDE010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.424"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHU0J_004348"
     CDS             <1..132
                     /locus_tag="ACHU0J_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1218702.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHU0J_004349"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHU0J_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHU0J_004350"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHU0J_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          287 a          176 c          252 g          213 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatag
//
LOCUS       DAZPDE010000418          923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPDE010000418 DAZPDE010000000
VERSION     DAZPDE010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.425"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..200
                     /locus_tag="ACHU0J_004351"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHU0J_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHU0J_004352"
     CDS             300..>923
                     /locus_tag="ACHU0J_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1218703.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZPDE010000419          918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPDE010000419 DAZPDE010000000
VERSION     DAZPDE010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.426"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            15..918
                     /locus_tag="ACHU0J_004353"
                     /pseudo
     CDS             15..918
                     /locus_tag="ACHU0J_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          287 a          141 c          189 g          301 t
ORIGIN      
        1 atcactcttt taatatcgct gtaattattc cttgttataa tgagcagaag gcaatagcaa
       61 aagtcataaa tgatttcaaa acgaatattc caactgcttc tatatatgta tttgataata
      121 attcgactga cagtacagca caagtggcag aggatgctgg agcaacagtt cactctgttc
      181 ctctgaaagg caaggggaat gtcgtaagac gtatgttctc agatgtagat gcggatattt
      241 atctaatggt tgatggagat gacacttatg atgcaagttc tgctccagaa atgattaacc
      301 atctcataaa aaatcaattg gatatggttg taggatgtcg tcaagagaat ggagatcaaa
      361 atacatacag aaaagggcat cgctgaggaa ataaattgtt gactggtact gttcagacta
      421 tctttaaagg gaattttact gatatgttgt cggggtatcg tgtattttcc agacgctatg
      481 ttaaatcttt tccatgtcta tcaaggggat ttgaaatcga aactgagctt acaattcatg
      541 ctttagaatt acgtatgaag tatggtgagg ttaatacaaa atatggcgag cgatctgaag
      601 ggagtgtgag caaactatca acttggagtg atgggttcaa gattctgaaa acaataatta
      661 aactttactc gcttgaacgg ccactatact tttttagtat tatcggcgtt ttacttgctg
      721 ctttgtctat aattttagga ttgcccatca ttgttgatta tattgataca ggtttggtga
      781 gaagatttcc gactgccttt ttaactgcct ccatcatgtt atcgtcaata atggcatttg
      841 tatgtggcat tattttgcat tcaaatacaa caactagacg tgaaatgaag gcattatttt
      901 atctttctga aaaaaact
//
LOCUS       DAZPDE010000420          917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPDE010000420 DAZPDE010000000
VERSION     DAZPDE010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.427"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..261
                     /locus_tag="ACHU0J_004354"
     CDS             <1..261
                     /locus_tag="ACHU0J_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1218704.1"
                     /translation="SNAIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHY
                     GLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            523..>917
                     /locus_tag="ACHU0J_004355"
     CDS             523..>917
                     /locus_tag="ACHU0J_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218705.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIV"
BASE COUNT          315 a          146 c          177 g          279 t
ORIGIN      
        1 agcaatgcta tagcacccac acctgtatat gaaaattatt ttaataacac tactgctttc
       61 agaccaaaac ctgaagatat cttcaaatgt ggacagacgc tcaaaaaaac taaacagaaa
      121 tttggccatt atggattagg ggtagaccag catcaaaaaa ttgataatta taccatgggg
      181 ctaaattctt ggaagatagc acctaacgaa cgtggtgata aaaaaggtgt cccagggctt
      241 agttgttttc aacctcaata aaacttcatg agtgctgtgg tatctctttt taattttgcc
      301 agtatttcat aggttttctt aatcgattgt agaggtatgt actgatgacg cctctacaat
      361 atttagggac tttctctgct atgagataag aaatttcata ttatgcttta aggctgaatg
      421 atttgtgcaa aatataatta tttccccatc gaatatgagg tgtatgtcat aagataaaat
      481 gttaactatg tattcctcgc ggtgaataat atgaggtaaa agatgaaaat tggaactgtg
      541 gcaggcacta atggcagtac aacgacaatc gcaacaaatg atatggtgca agagcatgtt
      601 accaacttca ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca
      661 agtattgcca ggacaatgct cggtgaagtt gtggaaaaaa ttgatgattg gcaaatagaa
      721 cgtttccaac aatctattca agatgacaaa atttcattta ccatacaaac gaatcattca
      781 gaaaaatata gtatgctatc gggcatgcgc gctcatattc tacgtaggaa taactgttat
      841 cagtttattg tgacaattaa tagtaaaaat tacggttgcc ctcttgataa taccgatatt
      901 aattggtgca gcattgt
//
LOCUS       DAZPDE010000421          913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPDE010000421 DAZPDE010000000
VERSION     DAZPDE010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.428"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..548
                     /gene="virA"
                     /locus_tag="ACHU0J_004356"
     CDS             <1..548
                     /gene="virA"
                     /locus_tag="ACHU0J_004356"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1218706.1"
                     /translation="GINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTS
                     LFQDPAFISALTSAFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHII
                     KINAVVPKNDTAISASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEG
                     LRNAIPPESRQVSFAIISPDV"
     gene            727..>913
                     /locus_tag="ACHU0J_004357"
     CDS             727..>913
                     /locus_tag="ACHU0J_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1218707.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          297 a          202 c          171 g          243 t
ORIGIN      
        1 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
       61 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
      121 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
      181 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
      241 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
      301 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
      361 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
      421 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
      481 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
      541 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
      601 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
      661 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
      721 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
      781 gttctggaaa gtcagggcga atatgactca caatgggcag cgatttgttc cattgcccca
      841 aagattggct gtatactgga aactctgcgt gtctgggtac gccagcatga gcgggatacc
      901 gggggcggtg agg
//
LOCUS       DAZPDE010000422          912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPDE010000422 DAZPDE010000000
VERSION     DAZPDE010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.429"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(167..>912)
                     /locus_tag="ACHU0J_004358"
     CDS             complement(167..>912)
                     /locus_tag="ACHU0J_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1218708.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          248 a          204 c          191 g          269 t
ORIGIN      
        1 tcatctctca tagtgtttaa attaacatat tttccaatat aaaaaataag actctggctt
       61 caattgtgcg cggattttct tacaggtgta ggataatagc cttcacaaaa taattatccg
      121 cgcaattttc gtgaatggag cgcatagact aaggtcaaaa tcacaattat tcatagataa
      181 aagtgacacc aatgactgac tggacggttc cggctgaaac cttgtttcct gtttgtccat
      241 aggttgcgct taaacccaga ggtactttgc ttttatttac tgtccccagg gagacatttt
      301 ctcccgtagc cagtattttt ccattacgca ttagagtgac acccactccc gatgcttttg
      361 ttgcatcagg tgctgtgtta gcaaacactt gtcggctact gtcagtggtg gcacccgaaa
      421 gataaaatga gagcttttgt tcgcttgagc aatatacgcc aaggggtatt tctgcgcttc
      481 caggaaagtc cgggagatcc acggtgacat tacgactatc aacggtgcag ccgcctgttg
      541 gcataacaac actgttattt gaaataatat tccacgtaaa attacgcgga tttccgcttc
      601 ctaatgttgc aattttatac atatgaatac gggcaatgac ttcccccgct tttatgacaa
      661 ctccaccggc cgccccaact ggggtaatat agagttttaa cggtaatggc atcggtgtct
      721 tatcaccaat atccaatacg ttggtatttg tcgtcaacgg gaaggggtag gtcacattat
      781 tccagtacag cgatccttta tatgactgta gggagcctgc gaaagctgag ccttgtacca
      841 ggtttatatg atcagtgtcg taccagccgc cgtaatcatt ccagcaactt atatgctgag
      901 acaagtctac aa
//
LOCUS       DAZPDE010000423          906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPDE010000423 DAZPDE010000000
VERSION     DAZPDE010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.430"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(130..202)
                     /locus_tag="ACHU0J_004359"
     tRNA            complement(130..202)
                     /locus_tag="ACHU0J_004359"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Ala,seq:tgc)
     gene            complement(254..>906)
                     /locus_tag="ACHU0J_004360"
     rRNA            complement(254..>906)
                     /locus_tag="ACHU0J_004360"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          215 a          246 c          217 g          228 t
ORIGIN      
        1 aaacttctgt agaaaattag gaatctgacc atgtgggcct tgcacacaag ggaagattgt
       61 ctattttcct aggaagtgag ttgcgtgttc aacttcactt gtattaagtt tagtaggatt
      121 gtatcctgat ggagcctagc gggatcgaac cgctgacctc ctgcgtgcaa agcaggcgct
      181 ctcccagctg agctaaggcc ccacaagacc tctcaaaact aaataagacg ataaacgtgt
      241 tccatttcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta ccttgttacg
      301 acttcacccc aatcatctat cccaccttag gcggctggct cctaataggt tacctcaccg
      361 acttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaaggc ccgggaacgt
      421 attcaccgcg gcgtgctgat ccgcgattac tagcgattcc gacttcatgt aggcgagttg
      481 cagcctacaa tccgaactga gattggcttt aagagattag cttgccgtca ccggcttgcg
      541 actcgttgta ccaaccattg tagcacgtgt gtagcccagg tcataagggg catgatgatt
      601 tgacgtcatc cccaccttcc tccggtttat taccggcagt ctcgctagag tgcccaactt
      661 aatgatggca actaacaata ggggttgcgc tcgttgcggg acttaaccca acatctcacg
      721 acacgagctg acgacaacca tgcaccacct gtcaccgatg taccgaagta aaactctatc
      781 tctagagcgg gcatcgggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
      841 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      901 tcgtac
//
LOCUS       DAZPDE010000424          903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPDE010000424 DAZPDE010000000
VERSION     DAZPDE010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.431"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            189..791
                     /gene="fimB"
                     /locus_tag="ACHU0J_004361"
                     /pseudo
     CDS             189..791
                     /gene="fimB"
                     /locus_tag="ACHU0J_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          265 a          160 c          194 g          284 t
ORIGIN      
        1 tggcgtttgt atggcaaagt tattataagt aactatcgct actccattta agttcgctca
       61 gaagaactgg tccacttacg taagttatta agcaaacgtt cgcttttata aacataatca
      121 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
      181 ggaaaacgat gaggaataag gctgataaca aaaaaaggaa cttccttacc catagtgaaa
      241 tcgagtcact ccttaaagca gcaaataccg ggcctcatgc aacacgtaat tattgtctga
      301 ttttgctttg ttttattcat ggtttccggg cgagtgaaat ttgtcgattg aggatttcgg
      361 atattgatct taaagcaaag tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa
      421 cgcacccgct attgaacaaa gaagttcagg ctttaaaaaa ctggttgagt atccgtactt
      481 cttatccgca tgctgagagc gagtgggtat ttttatcacg taaggggaat ccgctttctc
      541 ggcaacagtt ttaccatatt atctcgactt ctggtggtaa tgccgggttg tcactggaga
      601 ttcatccgca catgttacgc tattcgtgtg gttttgcttt ggcgaatatg ggaatagata
      661 cgcgacttat ctaggattat cttgggcatc gcaatattcg tcatactgtc tggtataccg
      721 ccagcaatgc agggcgtttt tacggcatct gggatagagc cagaggacga cagcgtcatg
      781 ctgttttata gagatagttg agataccagg gatggtgtta aaaactacat tattcttacg
      841 atatatcaaa aatgattaac catttattaa ttgatgttat tgtaagtgtg attcttaatt
      901 atc
//
LOCUS       DAZPDE010000425          897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPDE010000425 DAZPDE010000000
VERSION     DAZPDE010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.432"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..768
                     /gene="lpxM"
                     /locus_tag="ACHU0J_004362"
                     /gene_synonym="msbB"
     CDS             <1..768
                     /gene="lpxM"
                     /locus_tag="ACHU0J_004362"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1218709.1"
                     /translation="ARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELAI
                     RGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQ
                     QRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFAD
                     FFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIA
                     RQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          257 a          178 c          215 g          247 t
ORIGIN      
        1 gctcgtcgca gggcgacgat taatttatcg ctttgtttcc cggaaaagag tgatacagaa
       61 cgggaaataa ttgtcgacaa tatgtttgcc acagcattgc aatccatagt gatgatggca
      121 gaactggcaa ttcgtggtcc ggaaaagttc cagaaacgag tgttctggaa aggcctcgaa
      181 attcttgagg aaattcggca caataacaga aatgtgattt ttctggttcc ccatggctgg
      241 agcgtggata ttcctgcaat gttgctggca gctcaggggg aaaaaatggc cgccatgttt
      301 catcagcaac gaaatccagt gattgattat gtctggaatt ctgtacggcg taaattcggg
      361 gggcgcttac attcccggga ggatgggata aaaccattta ttcagtcagt acgccaggga
      421 tactgggggt attaccttcc agatcaggat catggtcctg aatacagtga atttgctgat
      481 ttttttgcga cctataaagc gacattacca attattggac gtctgatgaa catcagtcag
      541 gctatgatta taccgctttt cccggtttat gatgaaaaaa aacatttcct gactattgaa
      601 gttcggccac caatggatgc atgcattgcc agcgcggaca ataaaatgat tgcccgacaa
      661 atgaacaaaa cagtggaaat tttggtgggg tcacatccgg aacagtatat ctgggtttta
      721 aaattgttaa aaacgcgcaa atcaaacgaa gcggacccgt acccttgaaa ttataaagta
      781 caggtatttc cactagttgt ttcttacagg ttaccaatcg aaacacatcc cccttccgtg
      841 tgttgcccgt ttggcacgga ggactgtgta gatgcagttc gttaaattta atttatg
//
LOCUS       DAZPDE010000426          891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPDE010000426 DAZPDE010000000
VERSION     DAZPDE010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.433"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>891
                     /locus_tag="ACHU0J_004363"
     CDS             <1..>891
                     /locus_tag="ACHU0J_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR1218710.1"
                     /translation="DENKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVG
                     YLPGLAMRRIAALHAGEAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGF
                     DDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEK
                     KLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDE
                     VALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTR
                     RSGSSIRGEHPSRRGNKALKR"
BASE COUNT          185 a          295 c          233 g          178 t
ORIGIN      
        1 gacgaaaaca aactcaggtc gctaatatct gacctgaaac aacatggtca gatactgctg
       61 gttgttgatc agccagctac catcggtgcg ttacctgtcg ccgttgcccg ctcagaagga
      121 gtccttgtcg gatacctccc tggactggcc atgcgccgca tagccgcctt acacgccggt
      181 gaagctaaaa ctgatgctcg tgacgctgcc atcattgccg aagctgcccg taccctgcct
      241 cacgcgctac gcacgctgaa actggctgac gagcaaatcg ccgaactctc catgctctgc
      301 ggcttcgatg atgatcttgc cgcacagaca acgcaggcca gcaaccgtat ccgcggcctt
      361 ctgacccaga tacatccggc actggagcgc gttctcggtc cgagacttga gcacccggcg
      421 gtactcgatc ttctccagcg atatccctca ccagaaaaac tcgcttcgct gggtgagaag
      481 aagctggcag cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac
      541 atagctcagg cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta
      601 gtactgccac gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg
      661 cttgaggtag aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc
      721 ggagtcggtg tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc
      781 tctgccgcac atctcgctgc ttatgctggc cttgcgccgg taactcggcg atccggctcg
      841 tcaatacgcg gtgagcatcc ctcgcgacgg ggtaataaag ctctcaaacg g
//
LOCUS       DAZPDE010000427          888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPDE010000427 DAZPDE010000000
VERSION     DAZPDE010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.434"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..775
                     /gene="lpxM"
                     /locus_tag="ACHU0J_004364"
                     /gene_synonym="msbB"
     CDS             <1..775
                     /gene="lpxM"
                     /locus_tag="ACHU0J_004364"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1218711.1"
                     /translation="KKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAEL
                     AIRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMF
                     HQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEF
                     ADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKT
                     IARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          277 a          175 c          208 g          228 t
ORIGIN      
        1 caaaaaagct cgtcgcaggg cgacgattaa tttatcgctt tgtttcccag aaaagagtga
       61 tacagaacgg gaaataattg tcgacaaaat gtttgtcaca gcattgcaat ccatagtgat
      121 gatggcagaa ctggcaattc gtggtccgga aaagttccag aaacgagtgt tctggaaagg
      181 cctcgaaatt cttgaggaaa ttcgacacaa taatagaaat gtgatttttc tggttcccca
      241 tggctggagc gtggatattc ctgcaatgtt gctggcagct cagggaaaaa aaatggccgc
      301 catgtttcat cagcaacgaa atccagtgat tgattatgtc tggaattcag tacggcgtaa
      361 attcgggggg cgcttacatg cccgggagga tgggataaaa ccatttattc agtcagtacg
      421 ccagggatac tggggatatt acctcccaga tcaggatcat ggtcctgaat acagtgaatt
      481 tgctgatttt tttgcgacct ataaagcgac attaccaatc attggacgtc tgatgaacat
      541 cagtcaggct atgattatac cacttttccc ggtttatgat gaaaaaaaac atctactgac
      601 tattgaaatt cggccaccaa tggatgcttg cattgccagc gcggacaata aaacgattgc
      661 ccgacaaatg aacaaaacag tggaaatttt ggtggggcca catccggaac agtatgtctg
      721 ggttttaaaa ttgttaaaaa cacgcaaatc aaacgaagcg gacccgtacc cttgaaatta
      781 taaagtacaa gtatttccac tagttgtttg taagcgtaca gcctgaaccg tctggtcaga
      841 atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaac
//
LOCUS       DAZPDE010000428          887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPDE010000428 DAZPDE010000000
VERSION     DAZPDE010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.435"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            270..743
                     /locus_tag="ACHU0J_004365"
     CDS             270..743
                     /locus_tag="ACHU0J_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218712.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          270 a          186 c          197 g          234 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacgggg
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc
      301 agtgcactga taataaaaag acggagacag atgccattta cgataaagtt cggagttctt
      361 atttgctttc atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat
      421 catttgtttc tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact
      481 ggagtaattc cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa
      541 tgtacaaaga ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta
      601 tcaggaaatt acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca
      661 gttcaccatc gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca
      721 taaggaagaa aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt
      781 acgatactga gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac
      841 aaatctgaca gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DAZPDE010000429          880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPDE010000429 DAZPDE010000000
VERSION     DAZPDE010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.436"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            103..>880
                     /locus_tag="ACHU0J_004366"
     CDS             103..>880
                     /locus_tag="ACHU0J_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218713.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITT"
BASE COUNT          303 a          174 c          152 g          251 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtaa atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg
//
LOCUS       DAZPDE010000430          878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPDE010000430 DAZPDE010000000
VERSION     DAZPDE010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.437"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
BASE COUNT          276 a          177 c          156 g          269 t
ORIGIN      
        1 gtaacaagta aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt
       61 ctcaaatatg acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc
      121 agtaatttct tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc
      181 tggttcgtca gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg
      241 aacgcaggcg acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac
      301 ttaagccttc ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac
      361 atcatcctga aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt
      421 tacaagccac aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga
      481 gtaaaccctt attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat
      541 tctaaataaa ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca
      601 ccgcaagtgt tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt
      661 tgtgacgcga attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat
      721 aacaggtata aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca
      781 aacatcactt cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata
      841 tgaaaatgag atcaacagac actctatttt atgactct
//
LOCUS       DAZPDE010000431          873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPDE010000431 DAZPDE010000000
VERSION     DAZPDE010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.438"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>873
                     /locus_tag="ACHU0J_004367"
     CDS             <1..>873
                     /locus_tag="ACHU0J_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1218714.1"
                     /translation="NLSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVF
                     SKDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRL
                     MGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDA
                     MKKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGS
                     ENGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVT
                     LAYRPLNDTQRKIV"
BASE COUNT          265 a          153 c          209 g          246 t
ORIGIN      
        1 taatttaagt tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact
       61 atccgcttat cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat
      121 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
      181 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      241 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      301 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      361 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      421 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      481 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      541 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      601 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      661 tggtctgcaa gattttacaa gaagcttaac caatctttta aacgctcttg gtggaagtgg
      721 taagagtctt ggtgaatctc ttggtagtct catgacttct atgtcacatg gtgtcgattc
      781 attgaccgat attagttaca gggtaagggc gttctttgat gaggtgacat tagcttaccg
      841 tccactaaat gacactcaga ggaaaattgt tga
//
LOCUS       DAZPDE010000432          851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPDE010000432 DAZPDE010000000
VERSION     DAZPDE010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.439"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHU0J_004368"
     CDS             <1..318
                     /locus_tag="ACHU0J_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR1218715.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
     gene            complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHU0J_004369"
     CDS             complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHU0J_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR1218716.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT          222 a          183 c          218 g          228 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc gactttctgt ttacctgtaa ttaccccaat aaattcctgt acctgccttt
      421 gtttacgcgc cgacagctta cacaccttgc gtagtgactg catcagttcg ctctcttcgg
      481 cggcggcggg ttgggcggtg aggacaatac agccttccat cactttgaca tctaccgccg
      541 tgccggtggc aaaaccggcg gcttccagcc actgaccttt cagggtgatg gcgggaatac
      601 ggctgtaatc cgggtaacga ctcgcataac tcacggttaa ttgacgatta tttgccgggg
      661 agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc agtcatgatt actattctct
      721 ataaatagtg attgtgatta gcggtgcgag tgtgttggcg cacatccgca ccgtgctaaa
      781 tacctggata catcatcagt aaatacaggg aaagtccagc taaaaattaa tgaaaataag
      841 atgaaaatta g
//
LOCUS       DAZPDE010000433          839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPDE010000433 DAZPDE010000000
VERSION     DAZPDE010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.440"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>839)
                     /gene="ospC4"
                     /locus_tag="ACHU0J_004370"
     CDS             complement(<1..>839)
                     /gene="ospC4"
                     /locus_tag="ACHU0J_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR1218717.1"
                     /translation="SEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEA
                     PSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNL
                     SNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHH
                     TVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVH
                     GNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVF
                     QLE"
BASE COUNT          279 a          135 c          141 g          284 t
ORIGIN      
        1 tactcaagct gaaacacaaa gtttataatt ctatccagac taacaggatc aatatttttg
       61 ttataacaaa actctctgaa tccttgatct ttactctttc ttataaaatc aataagatgc
      121 aacccaattc caagcctcat atctttagtg ttaaatattg gaacatcagt tttcccctga
      181 ttaccatgca catacctgct aacttcatcc acaacctctt taaagttatc taagaaaaat
      241 gattggtgct catggtaaaa aacaggtgga atagcattat caaagtgatc ggttaatgtc
      301 atatatccat atggagagtc atgatcaatg atatacgcat tggcgccaaa atctacagta
      361 tgatgtgttt tatttagtgg gagtttttct tgatgattgg aaatttcgac cccaaaaaaa
      421 acaaaatcgt ggttagagat ttctttaata tcctcagagt atgtattctt atgtgtttgt
      481 atattttcag actgaatttt atcaaaggat ttaatattaa gaatattatt tgacagatta
      541 gagttacttt gatgtctgaa gttaaaatta gaattaataa taatagaaag taagtgacgt
      601 tcgtctgaag aaagaggatg aactttttgt attatctcag agatatagtg cctggacacc
      661 ttatcgagat gctcaagtga ccgaaatgat gaccgtgctc cagatggcgc ttcaagagaa
      721 agatttagtg atttgaaact ggtcccttga gaaaacattt tgctataact ttgcgctgct
      781 atagacttcc ttaaaaagtt tttcacagta ttagcacagt gatctaaatg tttttcact
//
LOCUS       DAZPDE010000434          836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPDE010000434 DAZPDE010000000
VERSION     DAZPDE010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.441"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(52..720)
                     /locus_tag="ACHU0J_004371"
                     /pseudo
     CDS             complement(52..720)
                     /locus_tag="ACHU0J_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            complement(710..>836)
                     /locus_tag="ACHU0J_004372"
     CDS             complement(710..>836)
                     /locus_tag="ACHU0J_004372"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR1218718.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          271 a          215 c          186 g          164 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aatatggcag
       61 tgctattttg attcgacgtt gatcaagttt ctgataattc caacgacggt cgacgggcac
      121 catccagtca atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg
      181 ccatgtcgtc agcgtcgaaa tggcgatgag taaaaaccat ccgccacaga ctttacgcca
      241 cctcacagcc agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag
      301 cgaaggagac tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcctgt
      361 caccccactc acttcaggac ggataaaact aaacttcagc gggtagagaa aaaaaccgca
      421 gcaggccatt accgttgcca gaataagccg gtggacaagt cggcgttgtt cgaatgtcga
      481 gctacaaacg aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt
      541 ccagggaaga aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct
      601 gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg
      661 ggccagcaac aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac
      721 gacgcaccgc catcgaaacc gtaaaaatgc cccacacatc aatccgttga gtgcatttat
      781 caaatccggc attacgcacg agcgaatcca tctccccttg cgaacgcacg cgcatt
//
LOCUS       DAZPDE010000435          835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPDE010000435 DAZPDE010000000
VERSION     DAZPDE010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.442"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            63..356
                     /gene="mxiI"
                     /locus_tag="ACHU0J_004373"
     CDS             63..356
                     /gene="mxiI"
                     /locus_tag="ACHU0J_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR1218719.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            362..>835
                     /gene="mxiJ"
                     /locus_tag="ACHU0J_004374"
     CDS             362..>835
                     /gene="mxiJ"
                     /locus_tag="ACHU0J_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR1218720.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKE"
BASE COUNT          280 a          126 c          173 g          256 t
ORIGIN      
        1 tgaaagtgat taaggatgtt gatgctgcaa ttattcaaaa cttcagataa tagggagcat
       61 tcatgaatta catttatcca gtcaatcagg ttgatattat caaagccagt gattttcaat
      121 ctcaagagat atcaagtctg gaagacgtcg tgtcggctaa atatagtgat attaagatgg
      181 atacagatat tcaagtatca caaataatgg agatggtaag caatccagaa tcattaaacc
      241 cagaatcttt ggccaagtta cagacgacgc tctcaaatta ttcaatagga gtatcattag
      301 ctggcacgtt agcaagaaaa acagtttcgg ctgttgaaac tttattaaag tcttaattta
      361 tatgattagg tataaaggtt ttattttatt cttgttgctg atgttgattg gatgtgagca
      421 acgtgaagag ttaatttcta atttatctca aagacaggca aatgaaataa tatctgtgct
      481 agaacgccat aatattactg ctagaaaagt tgatggaggt aaacagggga tctcggtaca
      541 agtcgaaaag gggacatttg catcggcagt tgatttgatg cgcatgtacg atttgccaaa
      601 tccggagaga gttgatatct cacaaatgtt tcctacagat tcattagtgt cttctccaag
      661 agctgaaaag gcccgtttat atagtgctat tgagcaacgg ctggaacagt ctttagtttc
      721 tattggtggt gttatttcgg caaaaataca tgttagctat gatcttgaag aaaaaaatat
      781 atcttcaaaa ccgatgcata tatcagtaat cgctatatat gactcaccga aagag
//
LOCUS       DAZPDE010000436          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPDE010000436 DAZPDE010000000
VERSION     DAZPDE010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.443"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            2..439
                     /gene="ipgC"
                     /locus_tag="ACHU0J_004375"
     CDS             2..439
                     /gene="ipgC"
                     /locus_tag="ACHU0J_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR1218721.1"
                     /translation="MSTAVIDAINSGATLKDINAIPDDMMDDIYSYAYDFYNKGRIEE
                     AEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKNDYTPVFHTGQ
                     CQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            445..>815
                     /locus_tag="ACHU0J_004376"
     CDS             445..>815
                     /locus_tag="ACHU0J_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HFR1218722.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKI"
BASE COUNT          298 a          163 c          123 g          231 t
ORIGIN      
        1 catctctact gcagtaattg atgcaattaa ctctggcgct acactgaaag atattaatgc
       61 aattcctgat gatatgatgg atgacattta ttcatatgct tatgactttt acaacaaagg
      121 aagaatagag gaagctgaag ttttcttcag gtttttatgt atatacgact tttacaatgt
      181 agactacatt atgggactcg cagctattta tcagataaaa gaacagttcc aacaagcagc
      241 agacctttat gctgtcgctt ttgcattagg aaaaaatgac tatacaccag tattccatac
      301 tggacaatgc cagcttcggt tgaaagcccc cttaaaagct aaagagtgct tcgaactcgt
      361 aattcaacac agcaatgatg aaaaattaaa aataaaagca caatcatact tggacgcaat
      421 tcaggatatc aaggagtaat tattatgcat aatgtaagca ccacaaccac tggttttcct
      481 cttgccaaaa tattggcttc cactgagctt ggagacaata ctatccaagc tgcaaatgat
      541 gcagctaaca aattattttc tcttacaatt gctgatctta ctgctaacca aaatattaat
      601 acaactaatg cacactcaac ttcaaatata ttaatccctg aacttaaagc accaaagtca
      661 ttaaatgcaa gttcccaact aacgctttta attggaaacc ttattcaaat actcggtgaa
      721 aaatctttaa ctgcattaac aaataaaatt actgcttgga agtcccagca acaggcaaga
      781 cagcaaaaaa acctagaatt ctccgataaa attaa
//
LOCUS       DAZPDE010000437          805 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPDE010000437 DAZPDE010000000
VERSION     DAZPDE010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.444"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHU0J_004377"
     CDS             complement(110..577)
                     /locus_tag="ACHU0J_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1218723.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..>805)
                     /locus_tag="ACHU0J_004378"
     CDS             complement(643..>805)
                     /locus_tag="ACHU0J_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR1218724.1"
                     /translation="TAIEYALIGVAMATLLAFIFGDQNSGFLGAIKDAFDAIAAAIQQ
                     VTISGTSNP"
BASE COUNT          287 a          170 c          158 g          190 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagta
//
LOCUS       DAZPDE010000438          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPDE010000438 DAZPDE010000000
VERSION     DAZPDE010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.445"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="ACHU0J_004379"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="ACHU0J_004379"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1218725.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          202 a          210 c          176 g          215 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcggga acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atc
//
LOCUS       DAZPDE010000439          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPDE010000439 DAZPDE010000000
VERSION     DAZPDE010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.446"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHU0J_004380"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHU0J_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZPDE010000440          796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPDE010000440 DAZPDE010000000
VERSION     DAZPDE010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.447"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(96..701)
                     /locus_tag="ACHU0J_004381"
     CDS             complement(96..701)
                     /locus_tag="ACHU0J_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1218726.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          225 a          182 c          133 g          256 t
ORIGIN      
        1 cttcatctgc gtgtcaccgg tatcccatat ttttgggatg tggctataat tatctattcc
       61 cgctatttag tcttcaaaaa aaattacatt taatatcatt tatcttgatt aagaaatagt
      121 aagactaaaa aaacaagcgt cgtcagccag cacactgcta acacggtaag aacgctaata
      181 aaaacactcc actgcttcgt tctataccat tcaatatttt tgtcagtatg ttttattggg
      241 agcagctctt cagtagagaa ttcctggctg ttatcattct caggaatgtt tgcatcagtg
      301 tttacttccc tggcctcgct atcgttggta tagctactac tgtcttcatc actgtcacgc
      361 tcattctcac agacatgttt ctcgaccttt cccagatatc tgacgccttt tcgcggaatg
      421 gttttaatta gagaactcga caaacctgcg acgtctaatg cctttctaag catgtaaatc
      481 tgcccataat aactgctgtc gcttaccgaa cctttctgct cccgccaaac ctcattgata
      541 atatgttgct tatcggattc tccctgtagt aaaaggagta aaaatcgata gccgttttca
      601 gacagattga ttacattgcc tgtactggtg gtgatacgtc gagacgctgg gtaaaactta
      661 actccctgcc ctaaagaaaa ataaaaacgg tcatcgctca taagtaccga cccctgtttt
      721 cttcttcaca tgactactca ttacaacttt gtatgaagta agatgcaaaa tcatattgtt
      781 tacattaagt taagca
//
LOCUS       DAZPDE010000441          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPDE010000441 DAZPDE010000000
VERSION     DAZPDE010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.448"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..789
                     /locus_tag="ACHU0J_004382"
                     /pseudo
     CDS             1..789
                     /locus_tag="ACHU0J_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family peptidase"
BASE COUNT          294 a          123 c          165 g          207 t
ORIGIN      
        1 ccactggtac tcaagaaaaa ataactaaag acaaagatat tgtgttcaca aatgggggaa
       61 gtgttctgtt taaggataat ttggattttg gttgcggtgg tattatcttt gacgaaggcc
      121 atgaatataa cataaacggt cagagattta catttaaagg agcaggaatt gatatcggaa
      181 aagaaagcat tgtaaactgg aatgcattgt attccagtga tgatgtttta cacaaaatag
      241 gccccggtac tctgaatgtt caaaaaaaac agggggcaaa tataaagata ggtgaaggaa
      301 atgttattct taatgaagaa ggaacattta acaatatata ccttgcaagc ggaaatggta
      361 aggtaatact aaataaagat aattcccttg gcaatgatca atatgcgggg atatttttta
      421 ctaaacgtgg tggcacgcta gatttaaatg gacacaatca gacttttact agaattgccg
      481 ccactgacga tggaacaaca ataactaact cagatacaaa gaaagaagcc gttctggcaa
      541 tcaataacga agtctcctac atatatcatg ggaacataaa tggcaatata aaactaacgc
      601 acaatattaa ttctcaggat aagaaaacta atgcaaaatt aattctggat ggtagtgtca
      661 acacaaaaaa tgatgttgaa gtcagtaatg ccagtcttac catgtaaggc catgcaacag
      721 agcatgcaat attcagaagc acagcgagtc attgctccct ggtatttctt tgtggaacgg
      781 actgggtca
//
LOCUS       DAZPDE010000442          777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPDE010000442 DAZPDE010000000
VERSION     DAZPDE010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.449"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..309)
                     /locus_tag="ACHU0J_004383"
     CDS             complement(<1..309)
                     /locus_tag="ACHU0J_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR1218727.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     L"
     gene            623..>777
                     /locus_tag="ACHU0J_004384"
     CDS             623..>777
                     /locus_tag="ACHU0J_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFR1218728.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKI"
BASE COUNT          245 a          157 c          117 g          258 t
ORIGIN      
        1 aagtttatag gtatctaatc gttccatcat ggctttaatt tttgacgtat caccacctgc
       61 caactttatt gcctcgcaaa aaacttcagc tctgtcacgc gcaaacaaaa ggctttttcc
      121 tgttaagctt gtttggttct gtaaatataa tcgattaagc gcttcgatcc ccttatttct
      181 ttctccattt ttaactcttt gatatgaaat cacgcttaca cctttgtgct ttccggtttc
      241 ttctgcgcct ttataggaat ctccgctttg aaatgaaacc tgatgcaatc ccaaattagt
      301 tatgtgcatt taattatcct cagtcataaa tggggcattg attgcagttt tgtacctcgt
      361 tttacaacaa aaaagatact tattcaacag ggttctctct ttttctataa cactccgagc
      421 ctgatacatc aggcacttta tcttgtggga tttcatgatg aaacgagcac tacatatgct
      481 cacgaggtac atattatgta cagcaaaaga aaatttgatt atgttaacag gctcaagttt
      541 ctaaatttat taattgaata tattatatac caaatacatt ttacatcccc acaatcacca
      601 agtaatggag agttaataaa gtatgatcct caaaattagc aatttcattg acgcaagcaa
      661 tacaaaaggg cctatacgcg ttgaagatac agagcatgga cctatattga tagcacagaa
      721 attcaaccta aaggatctct ttttcagaac attaagcact attaatgcaa agataaa
//
LOCUS       DAZPDE010000443          767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPDE010000443 DAZPDE010000000
VERSION     DAZPDE010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.450"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>767
                     /locus_tag="ACHU0J_004385"
     CDS             <1..>767
                     /locus_tag="ACHU0J_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218729.1"
                     /translation="EVLFILMEKYKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYS
                     DIIQVYGKILNMCNLSQETILTLLAAVGANNVSGLCMSFMNGHVDTIKAYGEIVFKTP
                     LTSDKRLYLLAAKDSHDLPGLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKH
                     GLFMALQNGHTKAIMAYGDILKILPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETI
                     RAFCNILKNYSFTTRRLVEMLSATNKDGIPGVFVSVV"
BASE COUNT          262 a          124 c          148 g          233 t
ORIGIN      
        1 cagaagtatt atttatactg atggaaaaat acaaaaatgg tgaaatttct caatctaaac
       61 tggtgaacct tctggccacg agaagctctg atggtactcc tgcattttat atagcacttc
      121 aaaacggata ttcagacatt attcaagtat atggtaagat attaaatatg tgcaatctta
      181 gtcaagaaac aatacttaca ttgcttgctg cggtaggggc aaacaatgtg tcgggtttat
      241 gcatgtcttt tatgaacgga catgtagaca caataaaagc ctatggggaa atagtcttta
      301 aaaccccgtt aacttccgat aagagattat atctattagc tgcgaaagac agccatgatt
      361 tacctgggtt attttttgca ttgcagaatg gacatgcaga tagtattcgt atgttcggaa
      421 gtctgcttaa taagaaaatg cttagtagtg aacaaattaa agaattattg aaagtaaagc
      481 atgggttgtt tatggcactt caaaatgggc acacaaaggc cattatggcg tatggagata
      541 tactaaaaat actcccccct caccaggaat atatagatga attattatgg ataaaaaatc
      601 ccaacggtac atcaggactt tttatggcat tttataatgg ccataccgaa acaatacgtg
      661 ccttttgtaa tattctaaaa aactatagtt ttaccacaag acgattggtc gaaatgctgt
      721 ccgcaactaa taaagatggt attcctggtg tatttgtatc tgtagta
//
LOCUS       DAZPDE010000444          760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPDE010000444 DAZPDE010000000
VERSION     DAZPDE010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.451"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>760
                     /locus_tag="ACHU0J_004386"
     CDS             <1..>760
                     /locus_tag="ACHU0J_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1218730.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          210 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atccatcggt tactcctatt ctaaagcact ggacgtagca
//
LOCUS       DAZPDE010000445          754 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPDE010000445 DAZPDE010000000
VERSION     DAZPDE010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.452"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHU0J_004387"
     CDS             complement(<1..158)
                     /locus_tag="ACHU0J_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1218731.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHU0J_004388"
     CDS             complement(308..469)
                     /locus_tag="ACHU0J_004388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218732.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHU0J_004389"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHU0J_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          200 a          218 c          146 g          190 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagc
//
LOCUS       DAZPDE010000446          732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPDE010000446 DAZPDE010000000
VERSION     DAZPDE010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.453"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(153..305)
                     /locus_tag="ACHU0J_004390"
                     /pseudo
     CDS             complement(153..305)
                     /locus_tag="ACHU0J_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            361..>732
                     /gene="ospZ"
                     /locus_tag="ACHU0J_004391"
     CDS             361..>732
                     /gene="ospZ"
                     /locus_tag="ACHU0J_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HFR1218733.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRAEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHA"
BASE COUNT          236 a          138 c          145 g          213 t
ORIGIN      
        1 agttatcaag catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac
       61 atataaaacg tctttattga tcctacccac ataatatgga cgcagtcgta agtgaggctc
      121 tgtgagccta cccacgtaat atggacacag gcctaagcga ggttctggtt ttcaaattgt
      181 tccggactga ggccgccaca ccaacggtgc cgtcgccagc gattgtaatt gcactcgata
      241 taattaaaca ctgttgcccg cactatttcc cgatagataa agtgttctcc gtggagacat
      301 tccacttagg aacaattata ttgttcatgg taatgtttat atttgagtat agagattaaa
      361 atgattagtc ccatcaagaa tattaagaac gtatttccaa taaatactgc aaatactgaa
      421 tatatagtga gaaatattta tcctagagtt gaacatggtt actttaatga atcaccaaac
      481 atatatgaca agaagtatat ctctggtata accagaagta tggcacaatt aaaaatagag
      541 gagtttatta atgagaaatc cagacgactt aattatatga agacaatgta ttctccatgt
      601 ccggaagatt ttcagcctat ttccagagcc gaagccagta caccggaagg aagctggttg
      661 acagttatat ctggaaaacg tcctatgggg caattttctg tggatagttt gtaccatcct
      721 gacttacatg ca
//
LOCUS       DAZPDE010000447          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPDE010000447 DAZPDE010000000
VERSION     DAZPDE010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.454"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>726)
                     /gene="ipaH7.8"
                     /locus_tag="ACHU0J_004392"
     CDS             complement(<1..>726)
                     /gene="ipaH7.8"
                     /locus_tag="ACHU0J_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR1218734.1"
                     /translation="PSINSNSTSNEHYLRILTEWEKNSSPGEERGIAFNRLSQCFQNQ
                     EAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNADNNRLSVIPELP
                     ESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLKFLHVYYNRLTTLPD
                     LPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPESFSQLDSSYRINIS
                     GNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          225 a          109 c          160 g          232 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggg
//
LOCUS       DAZPDE010000448          714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPDE010000448 DAZPDE010000000
VERSION     DAZPDE010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.455"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>714)
                     /locus_tag="ACHU0J_004393"
     CDS             complement(<1..>714)
                     /locus_tag="ACHU0J_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1218735.1"
                     /translation="QQFLFPLDYENKLQEQLDKYFTIESHPLLYRYTIGSKIANVIFE
                     KLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIALMSTFYDSDNT
                     SGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNL
                     FPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLVNLK
                     TLLNDGCFIKYSHFNDVALI"
BASE COUNT          239 a          135 c          105 g          235 t
ORIGIN      
        1 tattaatgcc acgtcattaa aatgactata tttaatgaaa caaccatcat tgagtagtgt
       61 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
      121 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
      181 atccacactt acgctacaaa tatcgtggta atgcgaagag tgcatttcag gaaacagatt
      241 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
      301 tgtttcaaaa ttatcatcag caaatccaag cactattgca tgctctacat ctaacgcagt
      361 ttcaaatgac tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
      421 gtcataaaaa gtgctcatca atgcgatatt gtttgttaca gcgtctttat cgatattctc
      481 actaatagca tatcgcctgc tataatcata aaaatgaata aatgcgtcct ttataaagga
      541 tatatattgt tctttgttaa agtcaatacg actgtataac ttttcaaaaa tcacattcgc
      601 aatcttacta ccaatggtat acctgtataa taatgggtgc gattcaatcg taaaatactt
      661 atctaattgt tcttgtaatt tattctcata gtccagtggg aaaagaaact gttg
//
LOCUS       DAZPDE010000449          708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPDE010000449 DAZPDE010000000
VERSION     DAZPDE010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.456"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            5..>708
                     /locus_tag="ACHU0J_004394"
     CDS             5..>708
                     /locus_tag="ACHU0J_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1218736.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT          227 a          141 c          143 g          197 t
ORIGIN      
        1 atatatgaat aaaatatact cccttaaata tagtgctgcc actggcggac tcattgctgt
       61 ttctgaatta gcgaaaagag tttctggtaa aacaaaccga aaacttgtag caacaatgtt
      121 gtctctggct gttgcaggta cagtaaatgc agcaaatatt gatatatcaa atgtatgggc
      181 gagagactat cttgatcttg cacaaaataa aggtattttc cagcccggag caacagacgt
      241 aacaatcact ttaaaaaacg gagataaatt ctctttccat aatctctcaa ttccggattt
      301 ttctggtgca gcagcgagtg gcgcagctac cgcaatagga ggttcttata gtgttactgt
      361 tgcacataac aaaaagaacc ctcaggccgc agaaactcag gtttacgctc agtcttctta
      421 caaggttgtt gacagaagaa attccaatga ttttgagatt cagaggttaa ataaatttgt
      481 tgtggaaaca gtaggtgcca ccccggcaga gaccaaccct acaacatatt ctgatgcatt
      541 agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa atcataggtt ttcgtgctgg
      601 ctctggagga acatcattta ttaatggtga atccaaaatc tcaacaaatt cagcatatag
      661 ccatgacctg ttaagtgcta gtctatttga ggtcacccaa tgggactc
//
LOCUS       DAZPDE010000450          707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPDE010000450 DAZPDE010000000
VERSION     DAZPDE010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.457"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..446)
                     /locus_tag="ACHU0J_004395"
     CDS             complement(<1..446)
                     /locus_tag="ACHU0J_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1218737.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDV"
BASE COUNT          216 a          173 c          131 g          187 t
ORIGIN      
        1 acatcatctg gataatcggc atgagcaata acatgcttgc cggtgcgact gtgtacttca
       61 agaataaagg gcaaatgctg acatttttcc agcgccgttt tcgcttgttt ctgttgatta
      121 tctgccagcg caataaacca gtcgccgcca ttcatcaacc acaaagacat ctgctggaat
      181 gccagcgcat ccatcgccat ctgttcatga ttgcctctta ccgcacgaac ccaatgttgt
      241 tccagtaact gcagacaacg taaactttgc ggcccacgat cgataacgtc tcccactgag
      301 ataagtaaat ctcgccacgg atcaaaacga caatgccata atttgcggcg caactgctca
      361 agacaaccgt gtatatcgcc agaaagccag atatgtcgcc attgatgacc cgcaattctc
      421 tgataaacgg gcgcaggctg tttcatcaat attttcctcc cgcgctaaag atcacataat
      481 cttaacaaga atgttaaaaa acgctggact cagacagtag agtgtgtgtt atggttgact
      541 ataaagtcag cgaaggaaat gcttctggct tttaacagat aaaaagagac cgaacatgat
      601 tcctgttttc gtcaacaaac aataaaaccc tttaaaatta atgcgttgaa taaatttacg
      661 ttcatctttt aatccctgac acgtaccatt gcatattaat acattca
//
LOCUS       DAZPDE010000451          700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPDE010000451 DAZPDE010000000
VERSION     DAZPDE010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.458"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(570..>700)
                     /locus_tag="ACHU0J_004396"
     CDS             complement(570..>700)
                     /locus_tag="ACHU0J_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1218738.1"
                     /translation="YFNKNMNIYTAYQINLLDKSDYVLAHGLNTDDQLAVGIVYQF"
BASE COUNT          188 a          108 c          187 g          217 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat
//
LOCUS       DAZPDE010000452          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPDE010000452 DAZPDE010000000
VERSION     DAZPDE010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.459"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            32..493
                     /locus_tag="ACHU0J_004397"
     CDS             32..493
                     /locus_tag="ACHU0J_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1218739.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>696)
                     /locus_tag="ACHU0J_004398"
     CDS             complement(534..>696)
                     /locus_tag="ACHU0J_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1218740.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          160 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatg
//
LOCUS       DAZPDE010000453          689 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPDE010000453 DAZPDE010000000
VERSION     DAZPDE010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.460"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..157
                     /gene="ipgA"
                     /locus_tag="ACHU0J_004399"
     CDS             <1..157
                     /gene="ipgA"
                     /locus_tag="ACHU0J_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR1218741.1"
                     /translation="SSICLLPDTHQCVLTKKHYKKYLSPDELIESLYEFLFCIKLTIA
                     NITSEVN"
     gene            173..>689
                     /gene="ipgB1"
                     /locus_tag="ACHU0J_004400"
     CDS             173..>689
                     /gene="ipgB1"
                     /locus_tag="ACHU0J_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR1218742.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNL"
BASE COUNT          253 a          127 c          108 g          201 t
ORIGIN      
        1 ttcttctatc tgtcttctcc ctgatactca tcaatgtgtt ttgaccaaga aacactacaa
       61 aaaatatctt tcacctgatg aactcattga atctttgtat gaatttttat tttgtattaa
      121 actaaccatt gcaaacatca cttcagaagt gaactaacat atagggggta tcatgcaaat
      181 tctaaacaaa atacttccac aggttgaatt cgcgattcct cgcccatcat ttgattcgtt
      241 aagtcgcaat aagcttgtaa agaaaatatt atctgttttt aacttaaaac aacgctttcc
      301 tcagaagaat tttggatgcc cggtgaatat aaataaaatc agggacagcg tcatagacaa
      361 aataaaagat agtaattcgg ggaatcaact attttgttgg atgagccaag agcgaaccac
      421 ttatgtctct tcaatgataa acagaagtat agatgaaatg gccatacaca atggagttgt
      481 tttgacctca gataacaaaa aaaacatatt tgcagctatt gaaaaaaaat tcccggatat
      541 aaaacttgat gaaaaatcag cacaaacatc tatcagtcat acagcactaa acgagattgc
      601 ctcatctggc ctcagagcaa agattttaaa acgctatagc agtgacatgg atttgtttaa
      661 cacacaaatg aaggatctta caaatctag
//
LOCUS       DAZPDE010000454          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPDE010000454 DAZPDE010000000
VERSION     DAZPDE010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.461"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHU0J_004401"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHU0J_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZPDE010000455          682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPDE010000455 DAZPDE010000000
VERSION     DAZPDE010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.462"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            114..314
                     /gene="glgS"
                     /locus_tag="ACHU0J_004402"
     CDS             114..314
                     /gene="glgS"
                     /locus_tag="ACHU0J_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1218743.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(357..>682)
                     /locus_tag="ACHU0J_004403"
     CDS             complement(357..>682)
                     /locus_tag="ACHU0J_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1218744.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
BASE COUNT          197 a          133 c          159 g          193 t
ORIGIN      
        1 gggacaatat atttacgcac gttatgttta aaggcactac actgattggg gaatactgaa
       61 atcagaaaaa gcatatggcc ttaaaaggta tgcgataacc ggaggatgtg cttatggagc
      121 atagtcttaa ttctttgaat aatttcgatt tcctggcgcg tagttttgcc agaatgcacg
      181 cagaaggtcg cccggtcgat attctggctg ttactggtaa catggatgaa gaacatagaa
      241 cttggttttg cgcacgttat gcctggtatt gccaacagat gatgcaggca agagagctgg
      301 agttagagca ctgatataac gggcctgatg gcccgtttta gtgtttgtat tagatgctag
      361 ttataggtga ttttgaggat tacgtcttta ctaaacggtc cctcaacaac agtctctgcc
      421 gggttcgcac taagttctgc cctgaatggt gcggtgaccg aaggtgcact agacgatgaa
      481 aaagaagcaa attgtgagaa gcggttaaat tccgttgctg ttccatcttt atgaaatatc
      541 ttcaatagca gtccattacc catatccaga tggctataat cactcaaaat atccgtagtg
      601 aaaaagctcc ctaaaatatt gaattgctca gtacaggccg caccaggatc ttttgtcatg
      661 actacactaa acgtctcact aa
//
LOCUS       DAZPDE010000456          681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPDE010000456 DAZPDE010000000
VERSION     DAZPDE010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.463"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..371)
                     /locus_tag="ACHU0J_004404"
     CDS             complement(<1..371)
                     /locus_tag="ACHU0J_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1218745.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDI"
     gene            447..596
                     /locus_tag="ACHU0J_004405"
                     /pseudo
     CDS             447..596
                     /locus_tag="ACHU0J_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          201 a          134 c          128 g          218 t
ORIGIN      
        1 ataatatctc cccatgaacc gttttcaaca aaataaataa tagcaggcac attaatagcc
       61 ttttcttctt cactcactga aacataattt ttaatttcat ccggaaaagc aaagtgatga
      121 taatccacgt gaccactact tttgttaaac tgtgctatcc agtaggaagc taaatgtcga
      181 cagacaataa gtcttccaga gtgatcggta acttggcaat ttagatttaa taaaaaactc
      241 ctcttgtcag aaaaatatgg caattgtttt gagtgcaata gagactggat tgtctcaaca
      301 gagacgttgt ctttatttat catattttgc aaacatattt tccttgatgg gattaaattt
      361 actgatggca tatgtatata ttcctttcat tgatgggttg tgacgcagac ttagtgttgt
      421 tgtactcttt gccctttgga tgattgatgg caaagattga tgtggtttgt caccgttact
      481 ctgaaactca ggggggtatc cggaacggcc attccagttc aggggggctg ctctatcgct
      541 gtaagcagtg cctgaagacc ttccagttcg actaccgcta caacggggcg aaacccatca
      601 aactttccaa ctattatcac attgagccat actgacaata aacaatatat cgatattttg
      661 tgcaaatact gccttttgct a
//
LOCUS       DAZPDE010000457          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPDE010000457 DAZPDE010000000
VERSION     DAZPDE010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.464"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHU0J_004406"
     CDS             complement(<1..251)
                     /locus_tag="ACHU0J_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR1218746.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACHU0J_004407"
     CDS             complement(272..>679)
                     /locus_tag="ACHU0J_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1218747.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZPDE010000458          678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPDE010000458 DAZPDE010000000
VERSION     DAZPDE010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.465"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..348)
                     /gene="mxiC"
                     /locus_tag="ACHU0J_004408"
     CDS             complement(<1..348)
                     /gene="mxiC"
                     /locus_tag="ACHU0J_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR1218748.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFK"
     gene            complement(367..>678)
                     /locus_tag="ACHU0J_004409"
     CDS             complement(367..>678)
                     /locus_tag="ACHU0J_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFR1218749.1"
                     /translation="PQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNIS
                     NIVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLN
                     Y"
BASE COUNT          204 a          129 c           99 g          246 t
ORIGIN      
        1 tttaaaatat ctctttagtc tgtctataaa atcccgatct agaggaaggt tatcttttaa
       61 cgttctcttt aaatcaaaaa tcttgtgatt aatttcatct tcttccccat ctaaaatacg
      121 ttcctgacta tcactatttg ttcctttcag tttctcaagg tctcttctgt ttataaatga
      181 cgaaagagcg gaagccatct catcagatga gtcaatatac ttgctattag ccaagccaga
      241 atcaagctct gcatcagcag tctcatctcc atcatctgaa tttgtcattg cattcaactt
      301 atcaatgaat gcagagctaa aaactcctgt atttttaaca tcaagcatac atcacctact
      361 ttatagttag taatttaagt atgaaaccaa tgatttttcg tcttccagca aagtagtttc
      421 agatgggatt atttgtgtag tcttttgtat ttctctttca gatatgaggc ttttatattc
      481 tgcagtatta taataacttg actcctttat ctcacggggt tgaatcagaa aaacccttac
      541 aatattactg atattacttg ttttatactt gaacacatta ccgattacag gaatagagga
      601 gagaaaagga atagatatta tttcgttaga gttcgtttca tgcgtataac cacctatcaa
      661 caaacttttg ccttgtgg
//
LOCUS       DAZPDE010000459          675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPDE010000459 DAZPDE010000000
VERSION     DAZPDE010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.466"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(29..397)
                     /locus_tag="ACHU0J_004410"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHU0J_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          205 a          118 c          145 g          207 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataatg ttatttttga tgcatacgcg ttataaatat caaaaataca aaccagataa
      661 atatcttatc aatat
//
LOCUS       DAZPDE010000460          673 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPDE010000460 DAZPDE010000000
VERSION     DAZPDE010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.467"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(10..140)
                     /locus_tag="ACHU0J_004411"
                     /pseudo
     CDS             complement(10..140)
                     /locus_tag="ACHU0J_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            138..221
                     /gene="tnpB"
                     /locus_tag="ACHU0J_004412"
                     /pseudo
     CDS             138..221
                     /gene="tnpB"
                     /locus_tag="ACHU0J_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          187 a          136 c          169 g          181 t
ORIGIN      
        1 cgcttccaat cagtagaata tgtgtttttc aatgaaggag ccgatgactt ttcgtggatt
       61 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg
      121 gtcaggttgt tacgttcaag atatagcatg tgctggatga agatcccttt tctggtcacc
      181 tgtttatctt tcgtggccga cggggtgaca ctgttaaaat gtaatgaacg catcccacac
      241 aattaaacac ggagtagtat gatggttaaa agggtgaaac tggcaggtgt ggagcctcgc
      301 agctttccgg catcatactg cccaatacgg gatgcataaa gcgtcctcag actggagact
      361 ccacaaatga aatatacacc ggttggcgtt gatatggcaa aacatgtcat tcagattcac
      421 ttcatcaatg agcacatgtc agcgccagtg atataagacg gtaatgggga tgcggacgat
      481 ttcctggact ctcagaagat gaaacatgtt tactgaagaa caaaaaattc gcgctattga
      541 gctttatttc aaatatggta agaagcttga ggtgtactgg caatagcgga caccaccatt
      601 tgttcttttt ctaagcagtc atctgataat attttatcct gaaggctgcc ggggagatat
      661 tccccagacg aga
//
LOCUS       DAZPDE010000461          665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPDE010000461 DAZPDE010000000
VERSION     DAZPDE010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.468"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(160..>665)
                     /locus_tag="ACHU0J_004413"
     CDS             complement(160..>665)
                     /locus_tag="ACHU0J_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1218750.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          175 a          165 c          146 g          179 t
ORIGIN      
        1 caccagcgcg tttaacgccc tgtgccattt ttcaggcaca aaaaaacccg cataaagcgg
       61 gttctttcaa gtgtccatgt ctgctattcg cctcgcgata cagctttgcg aagcttaccg
      121 taattgaagc agtttatgtg caaaaatgca agaacttttt taaagctgca tcaacctttc
      181 caccagttta tctctgcgaa caacaaacca accattggct ctcgccagtt ccagccatga
      241 ctcaagggaa ataacaatat catcatcccg taactgaatt gtggaaacag tgacaccgcc
      301 tctctgataa cagagaactc gcgtgtcgta acttttctgg catgaaactg gcgttgacgg
      361 atccctttga ctgaaatagc agtcttccag tttttcgaac acatcccacg cctgatcggt
      421 ttcgagcatt ttgccgtggc gtgctgcgcc tcgttctgtc cagaggatga gggagcgggc
      481 tttgggtgaa actggatttt gtgagtagtt taaagcgacc cgcaattctt taaggtcatt
      541 accaacaact ttgaaaaagt gtttcccttc aacgaagcgt actttgttct catgatgatt
      601 ctggcgaata cgcaccggct cagtgccgta aagctgcgcc aaaagttcgg tggtaataac
      661 aggaa
//
LOCUS       DAZPDE010000462          659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPDE010000462 DAZPDE010000000
VERSION     DAZPDE010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.469"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..534
                     /locus_tag="ACHU0J_004414"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACHU0J_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          157 a          178 c          160 g          164 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatct
//
LOCUS       DAZPDE010000463          658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPDE010000463 DAZPDE010000000
VERSION     DAZPDE010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.470"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHU0J_004415"
     CDS             <1..396
                     /locus_tag="ACHU0J_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218751.1"
                     /translation="NAAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQV
                     IPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTG
                     RNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            399..>658
                     /locus_tag="ACHU0J_004416"
     CDS             399..>658
                     /locus_tag="ACHU0J_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1218752.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR"
BASE COUNT          192 a          135 c          172 g          159 t
ORIGIN      
        1 aatgcagcgt tttatttgtg gggtaatcca tccaggcctg ttgttgcaga gcttggtgat
       61 gattcaggct ggcatttttt cagccagaga aacccggata acagcatagt gttcactgtt
      121 aacggacagg taattccgtt aaattacgga aacttcgatg cccgctataa atatcgaaca
      181 gagggggtac aggatgtacg gtatggccat gaaatgtatt acagccccgg cagtaacacc
      241 gtttcgtgga gattttgcgc accttcggga cacgggctgt cagggatggc gatatcggat
      301 accggccgta actcagcgga taacgttgac ggtgtgtatt accgaccact gcaaaaactg
      361 attaatggca cctggtataa cgtagcgagt atttaacaat gttgcattta aaaaatatta
      421 ctgcgggtaa tccgaaaaca gcagaacaat atcagatgac aaaacgatat tcggtcacct
      481 ggcttttttc agaagacgga aaaaactggt atgaagagct gaagaatttc gccagcgaca
      541 caataaaaat agcttacacc ggagatggtc gcgtggtgtg ggtcggtaag gatgtgacag
      601 gcatcgagcc acgcaatgcc agcgttatcg aagttccgga tattaccgct aaccgacg
//
LOCUS       DAZPDE010000464          657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPDE010000464 DAZPDE010000000
VERSION     DAZPDE010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.471"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            436..>657
                     /locus_tag="ACHU0J_004417"
     CDS             436..>657
                     /locus_tag="ACHU0J_004417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218753.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
BASE COUNT          128 a          204 c          153 g          172 t
ORIGIN      
        1 gttgcatttc gctgattatc aaaggtcttc acctcgttag gtcagttaat tcacgtcgca
       61 aacacattca aactgcttcc cttcgccatg taatgggctt tccccatcgc ggactactac
      121 ggaagctccg ccagccagcg cgtcatcgga gccatgcccc cttaacatcc gtcgctgacc
      181 ttccccggtt tacctgcctg gactcaggca tactgaggag gctgcccgtc gcactcttta
      241 tccttgcttg ccgcaagttg gcagaagtca gcaacgcaag tgtgatagac gctgctgccc
      301 cggtgtttcg catacatgtc aaaacacctt cgaccggcag tgcttacgta tcactgccag
      361 ttcctcctgc acggcctgtc agatcacgta ggccgtggtg acgttttcaa cccacagagg
      421 cggattaacg ggttcatgtt cttcagcctt tcagtactta accttgagga tcatctcggc
      481 ttagtgatct cgcctcaatc cccgttgtca gcgggttaca tcaccctgcg ggcatgccgc
      541 aggtcactgc cgctcaggtt ctccaccgtc acacccggtg ggattgttgg gtttctcatc
      601 gtgagttacc ggttcaatat tccagacaga ctcgcggttc atttaagcat ccatgcc
//
LOCUS       DAZPDE010000465          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPDE010000465 DAZPDE010000000
VERSION     DAZPDE010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.472"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHU0J_004418"
     CDS             <1..>656
                     /locus_tag="ACHU0J_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218754.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZPDE010000466          654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPDE010000466 DAZPDE010000000
VERSION     DAZPDE010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.473"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(146..358)
                     /gene="cspH"
                     /locus_tag="ACHU0J_004419"
     CDS             complement(146..358)
                     /gene="cspH"
                     /locus_tag="ACHU0J_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1218755.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          201 a          126 c          135 g          192 t
ORIGIN      
        1 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
       61 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
      121 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
      181 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
      241 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
      301 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
      361 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
      421 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
      481 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
      541 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
      601 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZPDE010000467          653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPDE010000467 DAZPDE010000000
VERSION     DAZPDE010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.474"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>653
                     /locus_tag="ACHU0J_004420"
     CDS             <1..>653
                     /locus_tag="ACHU0J_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFR1218756.1"
                     /translation="LPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSL
                     PSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQIT
                     TIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQ
                     MLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          198 a          163 c          102 g          190 t
ORIGIN      
        1 ctgccgttat taccagacac catcaaatca ctaagcgcag agtataatag gttatccaca
       61 ctgccttcat tacccttgaa tttaaaaaaa cttgaggtta ggaacaacga actgcaaact
      121 cttccatctc tgccttctaa tcttaagata cttaaggttg cgcacaacca tcttactgaa
      181 ctgccccctt tacctaggag actgcaactt ctttttgcat atagcaatag attaagcaac
      241 ttaccaaaca tccaagaaaa tattatcatg agaagatttt tttattttga aaacaaccaa
      301 ataactacaa tcccgacaaa tctttttcgt ttagatcctc atataactat tgagattgca
      361 aataacccct tatcagatca aactctgcta ttcttaatac agcaaacttc ggttccaaat
      421 tttaacgggc ctcagtttcg tatttccctg tcagaccaaa acagactgtt tttacgccag
      481 atgttgccgc aaaatttaca ttcgcgccat atcagagtca tcactgaagg ggggcagaac
      541 tttcagatcc cccctcttcc cgaaactgtg gcagcctggt ttcctgaagc agatcgtcgg
      601 gaggtttcta cacaatggac ttctttttcc accgaggaga attcccgggc att
//
LOCUS       DAZPDE010000468          648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPDE010000468 DAZPDE010000000
VERSION     DAZPDE010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.475"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..545)
                     /gene="fimE"
                     /locus_tag="ACHU0J_004421"
     CDS             complement(<1..545)
                     /gene="fimE"
                     /locus_tag="ACHU0J_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR1218757.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAG"
BASE COUNT          195 a          167 c          137 g          149 t
ORIGIN      
        1 aatccggcaa aacgagcagc attactggcg gtataacgca cagtatggcg aatatttcga
       61 tgcccgagat aatcctgaat taaacgggta tctgcaccac gctccgccag ttcataaccg
      121 caagcatgcc ttaacatatg aggatgagtc tgcgttacgg ttccagcttc aataccggca
      181 tcgcgaataa tgcgataggc ctgctggcga gaaagccgac tcccgcggcg agaaataaat
      241 atagcgtcgg tccggtcagc gcctttccag ttagcacgtt cctgggtcca gcgttccacg
      301 gcttcacgct catcaaaacg taacgggtga acggtagaaa atccgttctt cagtcggcga
      361 atatttattc gaccttcatt aaggtcaagg tcctgataat gcagatcaag cagttcacta
      421 atacgcatcc catgccgata tgccaacaga ataagacaat aatctctggc tcccgttgcc
      481 ccgtaacaaa ccgcctgcat catggcctga acttctttac cggtaagata acgacgttta
      541 ctcacaatag tagtactcct aactgagata tatttgaatg aatacctata ggaaacctca
      601 atcggtcaaa attagcccaa tagataaaaa gataaccaca cacgaagt
//
LOCUS       DAZPDE010000469          647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPDE010000469 DAZPDE010000000
VERSION     DAZPDE010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.476"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>647
                     /locus_tag="ACHU0J_004422"
     CDS             <1..>647
                     /locus_tag="ACHU0J_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1218758.1"
                     /translation="TKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVI
                     AQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASV
                     TGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNN
                     AIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          209 a          136 c          137 g          165 t
ORIGIN      
        1 aaactaaatt tacaggtggc atcactgcac ataacagcca aatagccata ggtgatcaag
       61 ctgtagttac acttaatggt gcaacctttc tgaataatac tcctataagt atagataaag
      121 gagcaaaagt tatagcacaa aattccatgt tcacaacaaa aggtattgat atctccggtg
      181 aactgactat gatgggaatc cctgaacaga atagtaaaac tgtaacgccg ggtctccact
      241 acgctgctga tggattcagg ctgagtggtg gaaatgcaaa tttcattgcc agaaatatgg
      301 catctgtcac cggaaatatt tatgctgatg atgcagcaac cattactctg ggacagcctg
      361 aaactgaaac accgactata tcgtctgctt atcaggcatg ggcagagact cttttgtatg
      421 gctttgatac cgcctatcga ggcgcaataa cagcccccaa agctacagtt agcatgaata
      481 atgcgatctg gcatctaaat agccagtcat caattaatcg tctagaaaca aaagacagta
      541 tggtgcgttt tactggtgac aatgggaagt ttacaaccct tacagtggac aaccttacta
      601 tagatgacag tgcatttgtg ctgcgtgcaa atctggccca agcagat
//
LOCUS       DAZPDE010000470          638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPDE010000470 DAZPDE010000000
VERSION     DAZPDE010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.477"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /gene="csgC"
                     /locus_tag="ACHU0J_004423"
     CDS             complement(<1..140)
                     /gene="csgC"
                     /locus_tag="ACHU0J_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR1218759.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(199..564)
                     /gene="csgA"
                     /locus_tag="ACHU0J_004424"
     CDS             complement(199..564)
                     /gene="csgA"
                     /locus_tag="ACHU0J_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1218760.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          145 c          154 g          173 t
ORIGIN      
        1 cgcagggaca atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata
       61 atggtgtaca tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca
      121 aggagtaata acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc
      181 tgtaatacaa atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg
      241 agtcacgttg acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc
      301 gaactgttta acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt
      361 accgaagcca cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc
      421 accattacca ccgccatgct gggtaatagt caagtcagag ttacgggcat cagtttgcag
      481 agcaagtgca gagttaccgc caccgtactg gtaaatgttc agctctgaat ttgggccgct
      541 attattactg aggtgtactg gcaatagcgg acactaccat ttgttctttt ttaagcagcc
      601 atctgatgat atttttccct gaaggctgcc ggggagat
//
LOCUS       DAZPDE010000471          636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPDE010000471 DAZPDE010000000
VERSION     DAZPDE010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.478"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHU0J_004425"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHU0J_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHU0J_004426"
     CDS             381..629
                     /locus_tag="ACHU0J_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1218761.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAZPDE010000472          634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPDE010000472 DAZPDE010000000
VERSION     DAZPDE010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.479"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            163..>634
                     /locus_tag="ACHU0J_004427"
     CDS             163..>634
                     /locus_tag="ACHU0J_004427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218762.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLL"
BASE COUNT          206 a           81 c          143 g          204 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttga
//
LOCUS       DAZPDE010000473          629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPDE010000473 DAZPDE010000000
VERSION     DAZPDE010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.480"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            61..360
                     /gene="pliG"
                     /locus_tag="ACHU0J_004428"
                     /pseudo
     CDS             61..360
                     /gene="pliG"
                     /locus_tag="ACHU0J_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
     gene            complement(468..>629)
                     /locus_tag="ACHU0J_004429"
     CDS             complement(468..>629)
                     /locus_tag="ACHU0J_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1218763.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          199 a          149 c          118 g          163 t
ORIGIN      
        1 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
       61 catagcagcg ctcagtactc cggcgaaata aagggatacg attacgatac atataccttc
      121 tatgccaaaa aaggtcagaa agtgcatgtg agtatctcta atgaaggcgc tgatacttat
      181 ctcttcgggc caggaatcga tgattctgtt gatttatcca gatattcccc agaactcgat
      241 agtcacggac aatactcact ccctgcttct ggcaaatatg agctgcgagt gctgcaaact
      301 cgaaacgacg cccgtaaaaa caaaactaaa aaatataacg tcgatattca aattaagtaa
      361 atgtcaaccg acggggacta tccccgtcat agacgttaac gccccactcc tctctgtcgc
      421 tcaagaggtt aatgacgaat agcagagaac ataatgtaaa caaggcatca ctcacgacac
      481 ataaataaca tctttgtggt ggtttgattg atttttccac tccgcttacc atctacacca
      541 aaataacgat catcacgaca taatttttct ttaacatcgc aaaatacacc gttagcgaat
      601 gtaaattcgc tgggatcgta gccttgtaa
//
LOCUS       DAZPDE010000474          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPDE010000474 DAZPDE010000000
VERSION     DAZPDE010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.481"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHU0J_004430"
     CDS             complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHU0J_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1218764.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          164 a          106 c          168 g          181 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgat
//
LOCUS       DAZPDE010000475          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPDE010000475 DAZPDE010000000
VERSION     DAZPDE010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.482"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            34..614
                     /locus_tag="ACHU0J_004431"
                     /pseudo
     CDS             34..614
                     /locus_tag="ACHU0J_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004243150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          176 a          111 c          134 g          193 t
ORIGIN      
        1 tcaggtaatt cttgttatag agaagcagtc gatattcata gctcacttga gctttcattt
       61 atattgcata aatatcattt gcgtgtagat gagaagaaat ctcgaaatgt attcagcgcc
      121 atacgtgatg agctcatcct tgatggggat tcacgacaaa acttagctac tttctgtcaa
      181 acatggcttg atgatgaaat ccgtgagttg cttaatttag ctattgataa aaacatgatt
      241 tataaagatg aatatccaca aatggcggaa cctgaaggtc gctgtgtgcg tatgctttcc
      301 gattcgtgga gtttccccga ttcgcgacac accttaggtt gctcaaccat tggttcatct
      361 gaagctgcta tgctcggccg cttggcgtta aaatggcagt ggtgtaaaaa acgcgaagta
      421 caaggtaaat caaccgaacc tgatttgcga tcctgtgcaa atatgctggc ataaattctg
      481 ccgttactgt gatgtgaaat tgtgtgaaat tccacttgat gatcatcacc tgataatgaa
      541 tgctaaagag gttttgaagc atgtcgatga aaacactatt ggtgtcgtta cgactttagg
      601 tattacttat tttt
//
LOCUS       DAZPDE010000476          601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPDE010000476 DAZPDE010000000
VERSION     DAZPDE010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.484"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..229
                     /locus_tag="ACHU0J_004432"
     CDS             <1..229
                     /locus_tag="ACHU0J_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR1218765.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          213 a          122 c           91 g          175 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaatga
      601 c
//
LOCUS       DAZPDE010000477          594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPDE010000477 DAZPDE010000000
VERSION     DAZPDE010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.485"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(70..594)
                     /locus_tag="ACHU0J_004433"
                     /pseudo
     CDS             complement(70..594)
                     /locus_tag="ACHU0J_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          164 a          142 c          121 g          167 t
ORIGIN      
        1 gctcatgggc acctctcttt aagccatctt aaatgactct gaggtgtctg ttaaacccgt
       61 ggcgattcag gtctgcatag cattatcaac attcggatgt gcaactacag ctaatgcaac
      121 atcatcccag cttgcaatag tgctatcagc aacaccatct ccgtcagtat ctacggtgac
      181 aaagttagat ttaccactat aatcgctggc attacctgta ttaccgaacc aaccagtagt
      241 accttcatca gtccatgaac cagaggttac ggtagaatct ttaacagtga tggtgttatt
      301 aaacacttcg ctgctgttta cgccagtgct gacagtgttg tcgtcaatat cttcccactc
      361 ataaccctga gtcaacgtaa tacccgcaac gtgagagtta ttagtgatgg acagatcaac
      421 ttcctgatcc agagtaatag ccgtaccaat gttgtaaacg tctacaacat gagtatcagc
      481 ggcattattg taagtgccgt tatatgtgta gtgttcgtag ttgtcgtcga tcgttgaatt
      541 atcaacagtc agcgccagac ggtcatggta gtaatcatcc gcacgatcag caca
//
LOCUS       DAZPDE010000478          569 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPDE010000478 DAZPDE010000000
VERSION     DAZPDE010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.486"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            97..477
                     /locus_tag="ACHU0J_004434"
     CDS             97..477
                     /locus_tag="ACHU0J_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR1218766.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT          184 a          109 c          123 g          153 t
ORIGIN      
        1 aaaaaatgca acagacagga atgcaataat tttgcattat ctcgttgctt gtaatgcaaa
       61 tatgcattat tgtcagtgtg tgacaataac agagagatga cgatggcgac cattaacgca
      121 cgtattgatg atgatataaa gaaccaagca gatgaagtgc tgaagctgat gaatatcagt
      181 caaactcagg ctattgcagc tttttatcag tatattactg aacaaaagaa actgccattt
      241 gtgatcacct caattgtaaa aacgccgcat gatttattac gtgaaagtac agacatgtta
      301 gctgaagctc ttgcagtgat ttccaatcta caggtgtgga ctgaacagca ggatgggatt
      361 ggaaaagcaa agctaatgga gtattaccgc cgcctcgacg cactgtactg ttgtgcaaaa
      421 gagaaaatag gtcttctttc agataaccgg gatgctgaac taggctgtgt cccttaatca
      481 tctgagttat agtaactgtc tgtttacaca acatattgaa ctatggctcg ctacgacctt
      541 cccgatgagg catggaccat catcaagcc
//
LOCUS       DAZPDE010000479          569 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPDE010000479 DAZPDE010000000
VERSION     DAZPDE010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.487"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            38..>569
                     /gene="virA"
                     /locus_tag="ACHU0J_004435"
     CDS             38..>569
                     /gene="virA"
                     /locus_tag="ACHU0J_004435"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR1218767.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDT"
BASE COUNT          221 a          113 c           86 g          149 t
ORIGIN      
        1 attggcatta ataggaaaat acatcaggag aaatcaaatg cagacatcaa acataactaa
       61 ccatgaaaga aatgactcat catggatgtc aactgtcaaa tcaactacag aagtaagttg
      121 gaataaattg agtttttgtg acattttact gaaaataata actttcggca tatattctcc
      181 acacgaaact ctagccgaaa aacatagcga aaaaaaacta atggatagtt tctcaccatc
      241 actatcacaa gataaaatgg acggagagtt tgcccacgca aacatagatg gcatttcaat
      301 acggctatgc cttaacaaag gcatatgttc ggttttttat cttgatggag ataaaataca
      361 atccactcaa ctttcatcaa aagaatataa taacttgtta agttcactcc cccccaaaca
      421 attcaactta ggaaaagtac atactattac agctccggta tcagggaatt ttaaaactca
      481 caagcctgca ccagaagtta ttgaaacagc cattaattgc tgtacatcga taataccaaa
      541 cgatgattac ttccacgtta aagacactg
//
LOCUS       DAZPDE010000480          568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPDE010000480 DAZPDE010000000
VERSION     DAZPDE010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.488"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHU0J_004436"
     CDS             <1..379
                     /locus_tag="ACHU0J_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFR1218768.1"
                     /translation="YDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEM
                     VISYYGGKISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERN
                     LSRLNEGMQYAKRHFTGIQTSCL"
     gene            321..>568
                     /gene="mxiN"
                     /locus_tag="ACHU0J_004437"
     CDS             321..>568
                     /gene="mxiN"
                     /locus_tag="ACHU0J_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR1218769.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIID"
BASE COUNT          189 a           72 c          130 g          177 t
ORIGIN      
        1 atatgatcta aattgcgata ttgaaccttt aagcggggta accgctatgt ttgttgccaa
       61 ctggaattta cttccagctg ttgcttattt tatcggtagt caagagtctc ggttaattaa
      121 tcatagtgag atggttatta gttattatgg gggcaaaatc agtaagcaag gggaggccgc
      181 tattcgaagt ggtttttggc atcttattgc gtggaaggaa aatatatctg ttggtatata
      241 tgaaagaata aacttattat ttaatccaat tgctttggag ggaaattata cgcctgtaga
      301 aagaaatttg tcccgattaa atgaaggtat gcaatatgca aaaaggcact ttaccggtat
      361 ccagacatca tgcctatgat ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa
      421 ccataaaaga tagagacact gagagtaaga agaaagcaat ttgtgttata aaggaggcta
      481 cgaaaaaagc tgaatcactg cgtatagatg ctgtatgtga tggttatcaa attggtatac
      541 agactgcttt tgagcatatt attgacta
//
LOCUS       DAZPDE010000481          554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPDE010000481 DAZPDE010000000
VERSION     DAZPDE010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.489"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            110..>554
                     /locus_tag="ACHU0J_004438"
     CDS             110..>554
                     /locus_tag="ACHU0J_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218770.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQ"
BASE COUNT          205 a          120 c           73 g          156 t
ORIGIN      
        1 tatttctgat agttataaac tgaaatttct taatcttgat tataacaagc tagcatcgct
       61 ggatgtctcc cggcaagaat ctctgattga gttaagtgcc catcacaata tgatcactga
      121 ccttatatta cacaatcatc ccaggatgaa aacaatcact ttaaacgaca accatattgc
      181 acatttaaac gccaaaaaca ctacaaaact ggaatattta aacttaagca ataacaattt
      241 actgccaacc aatgacattg atcaactaat atcatcaaaa catctttggc atgtattagt
      301 taacggcatc aacaatgatc cacttgcaca aatgcagtac tggactgcag taagaaatat
      361 aattgatgac actaatgaag tgaccattga tttatcagga cttaatttaa ccactcaacc
      421 accagggctg caaaacttca cctctatcaa tcttgataat aaccaactca cacattttga
      481 tgcaaccaac tacgatagac tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat
      541 aaattttcct caag
//
LOCUS       DAZPDE010000482          551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPDE010000482 DAZPDE010000000
VERSION     DAZPDE010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.490"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>551)
                     /locus_tag="ACHU0J_004439"
     CDS             complement(<1..>551)
                     /locus_tag="ACHU0J_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR1218771.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAZPDE010000483          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPDE010000483 DAZPDE010000000
VERSION     DAZPDE010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.491"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            174..>544
                     /locus_tag="ACHU0J_004440"
     CDS             174..>544
                     /locus_tag="ACHU0J_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218772.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDV"
BASE COUNT          190 a          113 c           86 g          155 t
ORIGIN      
        1 tatgtaaaac atcaaaaaaa gatgaaccaa atatacgaaa aaagcaaata tacttaccag
       61 ggtagaaagc atgatataac actcattccc ttatggcaaa tattctgaaa caccctcctc
      121 attaggagag atacattaaa tacaatttat tcacaatcta attccatatc catatgcgag
      181 aaattaatat gctcagaaac atttcatcct gtttatttcc acatatcagc acaattacat
      241 cccccaacca ttatttgtcc gaatgggatg attgggagaa acaggggtta ccggaagaac
      301 agcgtactga ggcggtaaga agacttcgtg catgtcttac ctctaagggg cataaactgg
      361 acctgcgagc cttggcgctt tcctcgctac ctgtactccc tgcttgcatt aaaaagcttg
      421 atgtgagctg taataaatta accatcctta ctgatttacc tgaaaatatt aaagaactta
      481 ttgcaagaga taatttctta acacatatat ctgcattacc acattatcta ataactttgg
      541 atgt
//
LOCUS       DAZPDE010000484          543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPDE010000484 DAZPDE010000000
VERSION     DAZPDE010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.492"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            392..>543
                     /locus_tag="ACHU0J_004441"
     CDS             392..>543
                     /locus_tag="ACHU0J_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1218773.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          135 a          143 c          118 g          147 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gagaccctag agtggctcct agagagaccc tcccagtagc
      241 tccgcacagc tacatcgtca tcatcatagt attcaatctc aaagtcattc tgtttgcatc
      301 ctgttgtcct gctggtctcc cttcgcttgg ggactttgag taggtcagtg ggggtgcgac
      361 ttcagggtgc aaacaaaaag gaaatcaagc gatgtctcaa caaccacaac aaccacaaca
      421 agaaatcagc atcattaacc tcgaccagct tgtgtccatg acctcagttg aaattgctga
      481 gctgacaggc aaggagcacc gaaatgtcct tcgtgatatt cgtaagatgc tcgaagaact
      541 taa
//
LOCUS       DAZPDE010000485          541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPDE010000485 DAZPDE010000000
VERSION     DAZPDE010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.493"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(54..>541)
                     /locus_tag="ACHU0J_004442"
     CDS             complement(54..>541)
                     /locus_tag="ACHU0J_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218774.1"
                     /translation="QFRWLAINNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMY
                     HLPLARSLVEHHAIVVEQYLRFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIAC
                     GLVGCSWEITKTYYPGIIATILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRK
                     Q"
BASE COUNT          220 a           99 c           84 g          138 t
ORIGIN      
        1 cacaagcaat tccaataaaa aaaccacata aaaaaattaa ttcattttgt gttttattgt
       61 tttctgagcg aatatatata agaatactgg ctgcagcaaa acaaagatag cgttaaatca
      121 atatatgcat aaccaagatg atctttaagt ggattgatag taaatagtag gattgtagca
      181 ataatgcctg gataataagt tttcgtaatt tcccatgaac agccaacaag tccacaggct
      241 attacaaaaa aacagatatt tgctaaaaac tgagctaacc ttacatctcc taactgtaaa
      301 cctaaagcca ttaaaagatc tgcattttgc gggaacagag gaaaacgcaa atattgttca
      361 acaacaattg catggtgctc aactaatgaa cgagccaaag gtaagtgata catagtatca
      421 tcccaatcac cgggagctcg cagggacgat aacaatgtaa ccacaaaaat caaaacaaga
      481 aataaaataa caaacagcgt actgaactca atattattta ttgccagcca tctaaattgc
      541 t
//
LOCUS       DAZPDE010000486          541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPDE010000486 DAZPDE010000000
VERSION     DAZPDE010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.494"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            185..260
                     /locus_tag="ACHU0J_004443"
     tRNA            185..260
                     /locus_tag="ACHU0J_004443"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Ile,seq:cat)
BASE COUNT          142 a          103 c          137 g          159 t
ORIGIN      
        1 tgctaattat taacatttgc ttgttgactg cgagtatcga ctttgcgaat ggaggcggtg
       61 tcgggtttta tgtcgcgtgt aatcacgacc caaggtgaat gggaacgcat aaaaatctgt
      121 tatttacatc ataagatcat catgtttatt gcgtggttta taagtattgt tattgcatac
      181 ttaaggccct ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc
      241 aagtccagca agggccacca aatcatcatt catgtatcag gaagagttaa gggtacaagg
      301 ggtactcgat ggcggtctat atttcatgac taacgccatt tcaaggaaga agtgcggtgg
      361 gtggtttcga atgagggctg gtgttttggt acgtggcacg ccactgcaat gtatttattc
      421 atcagtactc attcaaaagt acctggaaaa aatgcccgct atgccaagcg ggcaaattga
      481 tcctacccac gtaatatgga cacaggccta agcgaggttc tggttttcaa attgttccgg
      541 a
//
LOCUS       DAZPDE010000487          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPDE010000487 DAZPDE010000000
VERSION     DAZPDE010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.495"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHU0J_004444"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHU0J_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZPDE010000488          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPDE010000488 DAZPDE010000000
VERSION     DAZPDE010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.496"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>537
                     /locus_tag="ACHU0J_004445"
     CDS             <1..>537
                     /locus_tag="ACHU0J_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218775.1"
                     /translation="QQVTMLLTVAEGYQTKGDIKNAIHYADKALNMFDKAIAVDSSRE
                     SHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEGILLFYRNNDMQ
                     QDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRLAFLMTAPEYAC
                     YNSQYCYEDKVSALKILY"
BASE COUNT          189 a           93 c           96 g          159 t
ORIGIN      
        1 atcagcaagt caccatgtta cttacagttg cggagggtta tcaaacaaaa ggcgatataa
       61 aaaacgccat tcattatgcc gataaggcgc tgaatatgtt tgataaagct attgctgtag
      121 atagcagtcg tgaatcccat tatagtgatg agtatttgaa actcatgaat atctacgccg
      181 ccaatggcaa caaagaaaaa gcggtggttt tatctcaacg tctacacaaa gccattaccg
      241 aatctcaatc gaattactta agtactctgg aaggaatttt attgttttat agaaataatg
      301 atatgcagca agactatcaa aacagccttt caaattacat agtatttatc gataaaattt
      361 ttgccttttc cccatcaaca agaagtgaat taagcctcat taatttatta aattcattaa
      421 atgaggtgga attgatgaga aaacgtctgg cgtttctgat gacagcacca gaatacgctt
      481 gttataatag tcagtattgt tatgaagaca aagtcagcgc attaaaaatc ctgtatc
//
LOCUS       DAZPDE010000489          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPDE010000489 DAZPDE010000000
VERSION     DAZPDE010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.497"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>523
                     /gene="mxiD"
                     /locus_tag="ACHU0J_004446"
     CDS             <1..>523
                     /gene="mxiD"
                     /locus_tag="ACHU0J_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR1218776.1"
                     /translation="SLIAYPETNSILVKGNDQQIQIIRDIITQLDIAKRHIELSLWII
                     DIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALNQKKKAN
                     VVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQIEM
                     SLTIEDGTGNSQS"
BASE COUNT          173 a           78 c          105 g          167 t
ORIGIN      
        1 tatcacttat tgcatatcca gaaaccaatt ctatattagt caaaggtaat gatcagcaaa
       61 tacagattat tcgcgatatt atcactcagt tagatatagc caaacgacat atagaattat
      121 cattatggat aatcgatatt gataaatctg aattaaataa ccttggtgtt aattggcaag
      181 gaacggcttc atttggtgat tcgtttggtg cgtcatttaa tatgagctca tcagcatcga
      241 taagcacttt ggacggcaat aagtttattg cttcagttat ggcattaaat cagaaaaaga
      301 aagcaaatgt agtttcaagg ccggtgattt tgacacaaga gaatattccg gctatatttg
      361 ataataacag gactttttat gtgtcattag ttggtgagcg caattcttcg ttggagcatg
      421 ttacgtatgg aacattgata aatgttatac ctcgcttttc atctcgggga caaattgaga
      481 tgtctttaac gatagaggat ggaacaggaa atagtcagtc aaa
//
LOCUS       DAZPDE010000490          522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPDE010000490 DAZPDE010000000
VERSION     DAZPDE010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.498"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..249)
                     /locus_tag="ACHU0J_004447"
     CDS             complement(<1..249)
                     /locus_tag="ACHU0J_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR1218777.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQV"
BASE COUNT          135 a          128 c          117 g          142 t
ORIGIN      
        1 tacttgcatg tttaagatat ttttatttag ttgtttacgc gtaaatcgcc cggaatccag
       61 aggatcatta agtatcacat aaacaggttc cataccagcc ctgtccgcat caggacgcca
      121 gtaaataaaa tcttgtagtc caccggcatc agagacagga gtaatcgtcg ttaccggcat
      181 ttcgactggt tttatttccg tacctgtatg tactggtgtg agcggacaaa accactgtac
      241 gtctctcata cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
      301 cttcgggtaa aaccctcgtc gggaccactc cgaccgcgca caaaagcatt ttcatcgccg
      361 atagcgggta tggcttggca gggccgggat gggtataggt aatttggcta tcgtaaaaaa
      421 ggtggttaaa aattacaacc tgcaatgtaa attggctagt taatttttgg ctatttaaat
      481 tgacttctta acggcgttta ctgttccatt gctccccaac cc
//
LOCUS       DAZPDE010000491          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPDE010000491 DAZPDE010000000
VERSION     DAZPDE010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.499"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHU0J_004448"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHU0J_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZPDE010000492          506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPDE010000492 DAZPDE010000000
VERSION     DAZPDE010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.500"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>506
                     /locus_tag="ACHU0J_004449"
     CDS             <1..>506
                     /locus_tag="ACHU0J_004449"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR1218778.1"
                     /translation="DKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRF
                     CSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGN
                     DVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDEL
                     LRIKWWNY"
BASE COUNT          160 a           74 c          107 g          165 t
ORIGIN      
        1 tgacaagata acaattgaag ataatgttat tgctgaagaa tattcaacaa tgcctgtgaa
       61 aaatttctcc agtgtcggcg ctttttcatt tccgacctgc catttttcag ggaatatacg
      121 tataggtcga ttttgtagta ttgcatcaaa tgtaaaaata atgggtggaa atcatccttt
      181 gaatcgtttt actactcaca tgatgactta taacggtgaa tttgataagt ttgctatgag
      241 tgaatttgaa cgtagctgga cattgaaacc attcattacc aaacctgaaa atccgattat
      301 aggaaatgat gtctggatag gaaatgatgt tgtattgaaa ggtggcatcg caataggtga
      361 tggtgctgtt attgctgcca attccgttgt taccaaagat gtgcccccat atgccatagt
      421 tgcaggagtt cctgcaaaaa taatcagatt taggtttgat tcaaatgtta ttgatgaact
      481 gttgagaata aaatggtgga attata
//
LOCUS       DAZPDE010000493          492 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPDE010000493 DAZPDE010000000
VERSION     DAZPDE010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.501"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            149..>492
                     /locus_tag="ACHU0J_004450"
     CDS             149..>492
                     /locus_tag="ACHU0J_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218779.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT          154 a           76 c          106 g          156 t
ORIGIN      
        1 atgacattat tgttttttta tcgtccacgg ttacgcgacg ttgaagttac ttgttttata
       61 atgagcgatt tttgatatct cgccgagaaa attatcagca ttctctcgtt tcagtgtcgt
      121 gaatatactg ttaacggagg tgaaaagaat gctgggggtt atacaaaacc tatgttgttg
      181 tcgtccggat tcaccgcttg ctgatagtaa gcagcgtaac cagaagttac cgcaacaaga
      241 ctttgaggat tttaagaacg acgttattaa tgtcattaac aacaatgaag atatttattt
      301 tcttagagag agtaatttac tgaatgcgtt tattcggacg tgcaaaaaaa gagatgataa
      361 cagttacgga aatatgatat ttacgcttta ttttgataac gatatctatg ggaacagaat
      421 tatcaaagca tcgctgaacg gcggagagtt gcagttatta agcacagggt gtagcaatga
      481 taacattctc ca
//
LOCUS       DAZPDE010000494          488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPDE010000494 DAZPDE010000000
VERSION     DAZPDE010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.502"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHU0J_004451"
     CDS             <1..139
                     /locus_tag="ACHU0J_004451"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218780.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            133..488
                     /locus_tag="ACHU0J_004452"
                     /pseudo
     CDS             133..488
                     /locus_tag="ACHU0J_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          141 a          110 c          120 g          117 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt gcctgcggtt
      481 cagactgc
//
LOCUS       DAZPDE010000495          487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPDE010000495 DAZPDE010000000
VERSION     DAZPDE010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.503"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>487
                     /gene="ospB"
                     /locus_tag="ACHU0J_004453"
     CDS             <1..>487
                     /gene="ospB"
                     /locus_tag="ACHU0J_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR1218781.1"
                     /translation="NDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSH
                     QLGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILN
                     SLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPA
                     DP"
BASE COUNT          153 a           87 c           89 g          158 t
ORIGIN      
        1 taatgatgat gacgcattga atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa
       61 aacagtaaac gatactgatt ttaaagagta taataaggtt tatattcttg ggcacggtag
      121 tcctggttct catcaattag gccttggttc ggaacttatt gatgtacaaa caatcatttc
      181 aagaatgaaa gactgtggta ttctaaatgt gaaagatatc cgttttactt catgcggctc
      241 cgctgataaa gtggctccta aaaattttaa caatgcccct gctgaaagtc tttcttgtat
      301 ccttaactct ctgccttttt ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca
      361 ccttgaaaac gatgagtcat tgagtgatgg tctaaaaata tccggctatc atggatatgg
      421 agttcactat ggtcaagagc tttttcccta ctcacattat cgttcaactt caattcctgc
      481 tgatccg
//
LOCUS       DAZPDE010000496          485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPDE010000496 DAZPDE010000000
VERSION     DAZPDE010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.504"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..485
                     /locus_tag="ACHU0J_004454"
                     /pseudo
     CDS             1..485
                     /locus_tag="ACHU0J_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          178 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaaa
//
LOCUS       DAZPDE010000497          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPDE010000497 DAZPDE010000000
VERSION     DAZPDE010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.505"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..473
                     /locus_tag="ACHU0J_004455"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACHU0J_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAZPDE010000498          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPDE010000498 DAZPDE010000000
VERSION     DAZPDE010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.506"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHU0J_004456"
     CDS             3..>472
                     /locus_tag="ACHU0J_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR1218782.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZPDE010000499          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPDE010000499 DAZPDE010000000
VERSION     DAZPDE010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.507"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..361
                     /locus_tag="ACHU0J_004457"
     CDS             <1..361
                     /locus_tag="ACHU0J_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1218783.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZPDE010000500          470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPDE010000500 DAZPDE010000000
VERSION     DAZPDE010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.508"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>470
                     /locus_tag="ACHU0J_004458"
     CDS             <1..>470
                     /locus_tag="ACHU0J_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1218784.1"
                     /translation="PMELTPSCNEFYLKTWSEWEKNGTPGEQRNIAFNRLKICLQNQE
                     AELNLSELDLKTLPDLPPQITTLEIRKNLLTHLPDLPPMLKVIHAQFNQLESLPALPE
                     TLEELNAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          171 a           96 c           78 g          125 t
ORIGIN      
        1 cctatggaac tgactccatc ttgtaatgaa ttttatttaa aaacctggag cgaatgggaa
       61 aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctgaa aatatgttta
      121 caaaatcaag aggcagaatt aaatttatct gagttagatt taaaaacatt accagattta
      181 ccgcctcaga taacaacact ggaaataaga aaaaacctat taacacatct ccctgattta
      241 ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt acctgcctta
      301 cccgagacgt tagaagagct taatgcgggt gataacaaga taaaagaatt accatttctt
      361 cctgaaaatc taactcattt acgggttcat aataaccgat tgcatattct gccactattg
      421 ccaccggaac taaaattact ggtagtttct ggaaacagat tagacagcat
//
LOCUS       DAZPDE010000501          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPDE010000501 DAZPDE010000000
VERSION     DAZPDE010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.509"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>468
                     /locus_tag="ACHU0J_004459"
     CDS             <1..>468
                     /locus_tag="ACHU0J_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1218785.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAZPDE010000502          464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPDE010000502 DAZPDE010000000
VERSION     DAZPDE010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.510"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>464)
                     /locus_tag="ACHU0J_004460"
     CDS             complement(<1..>464)
                     /locus_tag="ACHU0J_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1218786.1"
                     /translation="NAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNV
                     LLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRS
                     FLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC"
BASE COUNT          146 a           87 c           66 g          165 t
ORIGIN      
        1 aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata atgcttttgt
       61 ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt aagtaaatct
      121 tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac cggataatca
      181 gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt gtcatcattg
      241 actggattat caactttgtc ttttaattca tagttaatac tatatcgggt attatatttc
      301 tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg atcaaggtgg
      361 gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga cgaaatgtta
      421 tttagattcc actcatcaat atttatcagt aaaaaaccag catt
//
LOCUS       DAZPDE010000503          462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPDE010000503 DAZPDE010000000
VERSION     DAZPDE010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.511"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHU0J_004461"
     CDS             complement(<1..170)
                     /locus_tag="ACHU0J_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218787.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYP"
     gene            complement(337..462)
                     /locus_tag="ACHU0J_004462"
                     /pseudo
     CDS             complement(337..462)
                     /locus_tag="ACHU0J_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          107 a          113 c          129 g          113 t
ORIGIN      
        1 gggtacattt tgtggaaggt ttcccagaaa cagaccgtgc aggagggatg accggtgata
       61 aagtctgcaa atacggactt ataagggtga ttattggctc tggcgacggc tttcagaacc
      121 tctttcacca ttctggtgtg gactttctgg tgctccaggt tgtgtgacat gggaactcat
      181 tctggatggt tactctgaaa gctcatattc tgccacaccc cgatttgcag ccgccaggct
      241 gccgtggttc acagataaaa cactctccag gaaacccggg gcggttcact gcgtgtctgg
      301 gttcgccagc atgagcggga taccgggggc ggttcacacg aacataccac agacagttga
      361 ttttggtacg cccaggcgac gtccggccac aacccgtggt aaatgttctt cgaaatgaag
      421 gcgtaaagct tcagtgatcc aggtccggta ttccatacga ta
//
LOCUS       DAZPDE010000504          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPDE010000504 DAZPDE010000000
VERSION     DAZPDE010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.512"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..461
                     /locus_tag="ACHU0J_004463"
                     /pseudo
     CDS             1..461
                     /locus_tag="ACHU0J_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          108 a          133 c          135 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc a
//
LOCUS       DAZPDE010000505          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPDE010000505 DAZPDE010000000
VERSION     DAZPDE010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.513"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHU0J_004464"
     CDS             complement(<1..>459)
                     /locus_tag="ACHU0J_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR1218788.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZPDE010000506          451 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPDE010000506 DAZPDE010000000
VERSION     DAZPDE010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.514"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
BASE COUNT          118 a           93 c           86 g          154 t
ORIGIN      
        1 ttatttagat aacattatct aactggacac tttcttgtat gtattatagc taaatgaaca
       61 gattttaata tgttgtatgg ttttcactct gaaatcccct gaatttaatc acgtttcctt
      121 tactgaaggc aattttaacc gtagtatcaa ttattaaccc ttgggcttga tatccctctc
      181 ctcttctggg cacgggggat gtcagattac ttattacaga aaccaaaaga attgtaagcg
      241 tcatctcagt gtcgtctatt tcaatgtctg atgattggcg atgatagagt cctcttataa
      301 cgttaatgga ctctatcaat gtcagattct cttcgccccc gcaggattcg tgcctcttac
      361 tctatgactc tatggagttt aagcttgatc ttattgaaaa atcttaccag ctaggggcct
      421 gcgtggccca actggccaga gagtacggga t
//
LOCUS       DAZPDE010000507          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPDE010000507 DAZPDE010000000
VERSION     DAZPDE010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.515"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..382)
                     /locus_tag="ACHU0J_004465"
     CDS             complement(<1..382)
                     /locus_tag="ACHU0J_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1218789.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          112 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcggt
//
LOCUS       DAZPDE010000508          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPDE010000508 DAZPDE010000000
VERSION     DAZPDE010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.516"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHU0J_004466"
     CDS             213..>443
                     /locus_tag="ACHU0J_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR1218790.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZPDE010000509          440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPDE010000509 DAZPDE010000000
VERSION     DAZPDE010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.517"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..149
                     /locus_tag="ACHU0J_004467"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHU0J_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(169..>440)
                     /locus_tag="ACHU0J_004468"
     CDS             complement(169..>440)
                     /locus_tag="ACHU0J_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR1218791.1"
                     /translation="KSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIK
                     SRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          111 a          106 c          103 g          120 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa cgacttttgc
//
LOCUS       DAZPDE010000510          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPDE010000510 DAZPDE010000000
VERSION     DAZPDE010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.518"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>439
                     /locus_tag="ACHU0J_004469"
     CDS             <1..>439
                     /locus_tag="ACHU0J_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1218792.1"
                     /translation="SAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSE
                     MADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSL
                     PAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGR"
BASE COUNT          112 a          105 c          131 g           91 t
ORIGIN      
        1 ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat atccctttat
       61 atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat accatggtgc
      121 gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg aatgactatg
      181 ttctggaggc aggaaaggtg cacgcagatg acactccggt gaaagtactg gccccgggga
      241 acggaaagac gaaaacgggt cgtctgtggg tatacgtcag ggatgatcgt aatgcgggtt
      301 catccctgcc ggcagccgtc tggttcgcgt attcggcaga tcgcaaagga gaacatccgc
      361 agctccacct ggcaaagtat cagggcgtac tgcaggctga tgcctatgca ggttataacg
      421 tactgtacga aacgggccg
//
LOCUS       DAZPDE010000511          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPDE010000511 DAZPDE010000000
VERSION     DAZPDE010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.519"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..96
                     /gene="ospE1"
                     /locus_tag="ACHU0J_004470"
     CDS             <1..96
                     /gene="ospE1"
                     /locus_tag="ACHU0J_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFR1218793.1"
                     /translation="TLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
BASE COUNT          158 a           79 c           79 g          122 t
ORIGIN      
        1 acgttgaaat tcactgctac agaaatgcaa tggttgacaa agataaatga agccggaatt
       61 gatgaaaaac aaagccaacg gtattctgat ttctgaagtt aatttcagac tcgtttaagc
      121 tccattttca aaaaaaatat atcagggttt gttactaata acttctgaaa acagaaaaaa
      181 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
      241 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtatag ggagagacgc
      301 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
      361 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
      421 ttaaaattaa taagccat
//
LOCUS       DAZPDE010000512          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPDE010000512 DAZPDE010000000
VERSION     DAZPDE010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.520"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>438
                     /gene="ospC1"
                     /locus_tag="ACHU0J_004471"
     CDS             <1..>438
                     /gene="ospC1"
                     /locus_tag="ACHU0J_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR1218794.1"
                     /translation="EVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIRKSED
                     QSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAVTASN
                     YEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQD"
BASE COUNT          165 a           64 c           83 g          126 t
ORIGIN      
        1 cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac
       61 caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta
      121 gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg
      181 atctggatag aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa
      241 gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag
      301 catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc
      361 tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg
      421 agataactag acaagatg
//
LOCUS       DAZPDE010000513          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPDE010000513 DAZPDE010000000
VERSION     DAZPDE010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.521"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>434
                     /gene="icsB"
                     /locus_tag="ACHU0J_004472"
     CDS             <1..>434
                     /gene="icsB"
                     /locus_tag="ACHU0J_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR1218795.1"
                     /translation="SLLLFLNTLASEKSAESAFAAYEAAKNSIQHSFTGRDIKLMLNT
                     AERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTHTYLSWYPGGNV
                     TKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQE"
BASE COUNT          154 a           90 c           80 g          110 t
ORIGIN      
        1 atccttattg cttttcctca atacattagc tagcgaaaaa tctgcagaaa gtgcgtttgc
       61 cgcctatgaa gcagctaaaa actcaattca acactctttc acaggtagag acatcaaatt
      121 aatgttgaat accgcagagc gttttcatgg catcggtaca gccaaaaatc ttgaaagaca
      181 tttagttttt cgttgctggg gaaatagagg cataacccat ttagggcata ctagtatcag
      241 tataaaaaac aatttactac aggaaccaac tcatacctat ctcagctggt atcccggcgg
      301 gaatgttaca aaagatacag aaataaacta cctttttgaa aaacgctcag gttacagtgt
      361 cgatacctat aagcaagaca aactaaatat gatttcagac caaacagccg aaagacttga
      421 tgcaggccaa gagg
//
LOCUS       DAZPDE010000514          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPDE010000514 DAZPDE010000000
VERSION     DAZPDE010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.522"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHU0J_004473"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHU0J_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZPDE010000515          431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPDE010000515 DAZPDE010000000
VERSION     DAZPDE010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.523"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>431)
                     /locus_tag="ACHU0J_004474"
     CDS             complement(<1..>431)
                     /locus_tag="ACHU0J_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1218796.1"
                     /translation="SDFDLTNCHFKGDVISKVSFLSSNLQHVTFECKEIENCNFTKAT
                     VDNVIFKCRRLHNVIFLKTSGECVDFSQNILDTVDFSQSQLGHSNFRECQIRNSNFDN
                     CYLYASHFTRAEFLSAKEISFIKSNLTAVMFDHVRMSTGNF"
BASE COUNT          158 a           75 c           65 g          133 t
ORIGIN      
        1 taaaaattcc ctgtcgacat tcgcacatga tcaaacataa cagctgtcaa attcgattta
       61 ataaatgata tttctttggc agacagaaac tctgctctgg tgaagtgcga agcgtaaaga
      121 taacaattat cgaagtttga atttctaatc tgacattcgc gaaaattact atgaccaagt
      181 tgactctgcg agaagtcaac tgtatcaaga atattttggc taaaatcaac acattcgcca
      241 ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt taaatatgac attatcaact
      301 gttgccttag taaaattgca attctcaatt tctttacatt cgaaagttac atgttgtaga
      361 tttgatgata aaaaagacac cttagaaata acgtcacctt taaaatgaca atttgttaaa
      421 tcaaaatcag a
//
LOCUS       DAZPDE010000516          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPDE010000516 DAZPDE010000000
VERSION     DAZPDE010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.524"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACHU0J_004475"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACHU0J_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR1218797.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZPDE010000517          410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPDE010000517 DAZPDE010000000
VERSION     DAZPDE010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.525"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            156..341
                     /locus_tag="ACHU0J_004476"
                     /pseudo
     CDS             156..341
                     /locus_tag="ACHU0J_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          119 a           97 c          108 g           86 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt
      241 tcgaagagta tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac
      301 gctcactcag cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca
      361 caaatccccg ggtggggatt tgtgtataag agacagccca tggaggaagg
//
LOCUS       DAZPDE010000518          410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPDE010000518 DAZPDE010000000
VERSION     DAZPDE010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.526"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            187..>410
                     /gene="ydeI"
                     /locus_tag="ACHU0J_004477"
     CDS             187..>410
                     /gene="ydeI"
                     /locus_tag="ACHU0J_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1218798.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT          129 a           78 c           81 g          122 t
ORIGIN      
        1 ttattctgaa aacatgttta agactcttca atcttcttaa ctttagtgag tcaaattctc
       61 tgaatatttg acagctagtg ggctgatttt aaatgcctta taacaattgg cagtattctt
      121 atttggcaat tataaaaata gcaaccagac ttaataagtc aaaccaaacg caattaagga
      181 gttagtatga aatttcagtc cattgtatta gcaagttttc ttgtcatgcc ttatgcgttg
      241 gcagatgacc agggcggttt aaaacaggat gcagcgccac cgccgcctca tgcaatagaa
      301 gatggctatc gcggaaccga tgatgcaaaa aaaatgaccg ttgatttcgc aaaaaacatg
      361 cacgatggcg cttcggtttc actacgcggt aatttgattt ctcacaaagg
//
LOCUS       DAZPDE010000519          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPDE010000519 DAZPDE010000000
VERSION     DAZPDE010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.527"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..>406)
                     /locus_tag="ACHU0J_004478"
     rRNA            complement(<1..>406)
                     /locus_tag="ACHU0J_004478"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           76 a          132 c           87 g          111 t
ORIGIN      
        1 ctctcccctt ctgcactcaa gttctccagt ttccaaagcg tacattggtt gagccaatgc
       61 ctttaacttc agacttaaaa aaccgcctgc gctcgcttta cgcccaataa atccggacaa
      121 cgctcgggac ctacgtatta ccgcggctgc tggcacgtag ttagccgtcc ctttctggtt
      181 agttaccgtc acttggtgga ttttccactc ccaccatcat tcttctctaa caacagagct
      241 ttacgatccg aaaaccttct tcactcacgc ggcgttgctc ggtcagggtt gcccccattg
      301 ccgaagattc cctactgctg cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg
      361 gccgatcacc ctctcaggtc ggctatgtat cgtcgccttg gtgagc
//
LOCUS       DAZPDE010000520          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPDE010000520 DAZPDE010000000
VERSION     DAZPDE010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.528"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(1..246)
                     /locus_tag="ACHU0J_004479"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="ACHU0J_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           91 a          128 c           89 g           91 t
ORIGIN      
        1 gtctccgtta tggcgtacac cacatcttcc agtaatcgcc gcttcaccgg actcaggacc
       61 ccgtatcacc atccggaacc ccttatccgc cacatgccag gagaaccttt ccataaactc
      121 gtcgccactc atcacaaggt tttcttctcg ttttgtccgg tggctgttgt aacgcaggcc
      181 aatttcatcc tgaccggcat aatgttccag gcggctcatc tgcaccggct gcttctccag
      241 ccagttaagc catcggtttc cctgctgaaa aatatcggca aaacggggaa gcatcagaaa
      301 ggcggaataa cgccatccgg ccagtgaacc gtaccgcatt cagggcaata catgccgtcg
      361 gcgttgatac cggcaggaat tgtcgcaaat tcccgctcc
//
LOCUS       DAZPDE010000521          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPDE010000521 DAZPDE010000000
VERSION     DAZPDE010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.529"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>393
                     /gene="spa40"
                     /locus_tag="ACHU0J_004480"
                     /gene_synonym="spaS"
     CDS             <1..>393
                     /gene="spa40"
                     /locus_tag="ACHU0J_004480"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR1218799.1"
                     /translation="MKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKSDI
                     RNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKL
                     ARKLYKTHTKYSFVDFEHLDEVLRLIVW"
BASE COUNT          138 a           58 c           81 g          116 t
ORIGIN      
        1 acatgaaaga tatgatgatg gataaacagg agataaaaag agaatatata gagcaagagg
       61 gacactttga gacaaagtcg agaaggcgtg agttgcatat cgagattctt tcagagcaga
      121 ctaaatctga tatacgtaat tcaaaattag tggtaatgaa cccgactcat attgcaattg
      181 gtatttattt taatccagaa atagcgcctg caccttttat ttctctcatt gaaactaacc
      241 agtgtgcctt ggctgtcaga aaatatgcaa atgaagttgg tataccgact gtgcgtgatg
      301 tgaaattagc tagaaaacta tataaaacac atacaaaata tagttttgtt gattttgaac
      361 acttggatga agtcctacgt cttattgttt ggc
//
LOCUS       DAZPDE010000522          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPDE010000522 DAZPDE010000000
VERSION     DAZPDE010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.530"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(43..>391)
                     /locus_tag="ACHU0J_004481"
     CDS             complement(43..>391)
                     /locus_tag="ACHU0J_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1218800.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           93 a           91 c           87 g          120 t
ORIGIN      
        1 accttcgtga agctgttgaa gggtggattc ttaacggtgg cgttaatcag aaatgctcct
       61 gatgtgccag aacaatttat cagcgtgaag ctcatacgct ttgcgctgag cctctatcca
      121 gtcgtgttta ttgtatacgg acattatccc gccaagttca tgaccgagca ttcgctcagt
      181 aacatgaggc tcgacattca tctcggacag ccgtgtaaca agcgttcttc tgaaatcatg
      241 tgttcgccag taacctagat ccatccctcc ccttattcta tttacgaatc ggttggctgc
      301 agcgatgctg atcggtttgt ttatgtcttc tccagggaaa agaacatcat tgtacattgt
      361 catggccttt tcgagaaaag gcttaatttg t
//
LOCUS       DAZPDE010000523          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPDE010000523 DAZPDE010000000
VERSION     DAZPDE010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.531"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>391
                     /gene="virF"
                     /locus_tag="ACHU0J_004482"
     CDS             <1..>391
                     /gene="virF"
                     /locus_tag="ACHU0J_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR1218801.1"
                     /translation="YSEEKRGLNKKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACL
                     LSAVSDEEALYTSISIASSLSFSDQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRL
                     ESEKLTFQQILLDIRMHHAAKLLLNSQS"
BASE COUNT          131 a           58 c           74 g          128 t
ORIGIN      
        1 tattctgagg agaaaagggg gttaaacaaa aaaatattcc tcctctctga ggaggaggtt
       61 tctatcgatt tgttcaaatc tataaaagag atgcctttcg gcaaaagaaa gatctatagt
      121 ttagcttgcc ttttatcagc tgtttctgat gaggaagctt tatatacttc gatatcgata
      181 gcttcttctc ttagtttttc tgatcagata aggaagattg ttgaaaaaaa catcgagaag
      241 agatggcgtc tttctgatat ttcaaataac ttgaatttat cagaaatagc tgttagaaaa
      301 cgattggaga gtgaaaaatt aacatttcaa caaatccttc ttgatattcg catgcatcat
      361 gcagcaaagc ttttattgaa tagtcaaagc t
//
LOCUS       DAZPDE010000524          388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPDE010000524 DAZPDE010000000
VERSION     DAZPDE010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.532"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            16..357
                     /locus_tag="ACHU0J_004483"
                     /pseudo
     CDS             16..357
                     /locus_tag="ACHU0J_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          127 a           60 c           81 g          120 t
ORIGIN      
        1 aataaagcta ataacatcca attaagatat gatgataagc agtttcatac aacggttatc
       61 aaagatgttc tattatggat tgaacataat ttagatcagt ctttactgct tgatgatgtg
      121 gcgaataaag cgggttatac caagtggtat tttcagcggc tgttcaaaaa agtaacaggg
      181 gtcacactgg ctagctatat tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg
      241 ttgacgaaaa aaactatcct tgagatcgca ttaaaatatc aatttgattc ccaacaatct
      301 tttacacgtc gatttaagta catttttaag gttacaccaa gttattatcg gcgtaataaa
      361 ttatgggaat tggaggcaat gcactgag
//
LOCUS       DAZPDE010000525          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPDE010000525 DAZPDE010000000
VERSION     DAZPDE010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.533"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHU0J_004484"
     CDS             <1..>375
                     /locus_tag="ACHU0J_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1218802.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZPDE010000526          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPDE010000526 DAZPDE010000000
VERSION     DAZPDE010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.534"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHU0J_004485"
     CDS             <1..>374
                     /locus_tag="ACHU0J_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1218803.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZPDE010000527          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPDE010000527 DAZPDE010000000
VERSION     DAZPDE010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.535"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..349
                     /locus_tag="ACHU0J_004486"
     rRNA            <1..349
                     /locus_tag="ACHU0J_004486"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           96 a           92 c          103 g           81 t
ORIGIN      
        1 tcaagtcatc atggccctta cgaccagggc tacacacgtg ctacaatggc gcatacaaag
       61 agaagcgacc tcgcgagagc aagcggacct cataaagtgc gtcgtagtcc ggattggagt
      121 ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg tggatcagaa tgccacggtg
      181 aatacgttcc cgggccttgt acacaccgcc cgtcacacca tgggagtggg ttgcaaaaga
      241 agtaggtagc ttaaccttcg ggagggcgct taccactttg tgattcatga ctggggtgaa
      301 gtcgtaacaa ggtaaccgta ggggaacctg cggttggatc acctccttac cttaaagaag
      361 cgttctttgt ag
//
LOCUS       DAZPDE010000528          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPDE010000528 DAZPDE010000000
VERSION     DAZPDE010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.536"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHU0J_004487"
     CDS             complement(<1..311)
                     /locus_tag="ACHU0J_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1218804.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZPDE010000529          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPDE010000529 DAZPDE010000000
VERSION     DAZPDE010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.537"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>366
                     /gene="gtrII"
                     /locus_tag="ACHU0J_004488"
     CDS             <1..>366
                     /gene="gtrII"
                     /locus_tag="ACHU0J_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR1218805.1"
                     /translation="IISAFIICRSLKLESYELLIGMLVFITFPQISYQLEFLNQADTV
                     GIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMAIYQTFVTYIIAFVIGLQINSIIR
                     NEKNIRESFYSSCLSLSLIA"
BASE COUNT          110 a           65 c           55 g          136 t
ORIGIN      
        1 catcatttca gcatttataa tctgcagatc gctaaagcta gaatcttatg aattattgat
       61 aggtatgtta gtatttatta ccttccctca gatctcctat caattagagt ttcttaacca
      121 agctgatact gtgggaattg cttttctact ggcagcgata tcagcaatta tttttcactc
      181 gcaaaaaaat aggattgtga tattttctgg tatagtactg tccattcttt caatggcaat
      241 ctaccaaaca ttcgtaacat atattattgc attcgtcatt gggttgcaga taaattcgat
      301 aatacgaaat gagaaaaata ttcgtgaatc tttttatagt tcatgtttat ctctatccct
      361 catagc
//
LOCUS       DAZPDE010000530          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPDE010000530 DAZPDE010000000
VERSION     DAZPDE010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.538"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            1..365
                     /locus_tag="ACHU0J_004489"
                     /pseudo
     CDS             1..365
                     /locus_tag="ACHU0J_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           76 a           95 c          114 g           80 t
ORIGIN      
        1 cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg ccgggcaacc
       61 gcccgcatta tgggcgttgg cctcaacacg attttccgcc atttaaaaaa ctcaggccgc
      121 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
      181 aacagtgggg atacgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
      241 ggctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc
      301 gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc
      361 tgtat
//
LOCUS       DAZPDE010000531          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPDE010000531 DAZPDE010000000
VERSION     DAZPDE010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.539"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHU0J_004490"
     CDS             <1..>365
                     /locus_tag="ACHU0J_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1218806.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPWKYPTAPKK"
BASE COUNT          111 a           69 c          113 g           72 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga
      361 aaaag
//
LOCUS       DAZPDE010000532          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPDE010000532 DAZPDE010000000
VERSION     DAZPDE010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.540"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHU0J_004491"
     CDS             <1..>365
                     /locus_tag="ACHU0J_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1218807.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZPDE010000533          363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPDE010000533 DAZPDE010000000
VERSION     DAZPDE010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.541"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            <1..>363
                     /locus_tag="ACHU0J_004492"
     CDS             <1..>363
                     /locus_tag="ACHU0J_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR1218808.1"
                     /translation="MIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPIQEVM
                     PIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKN
                     LKELADKKGQFIAIYRGFL"
BASE COUNT          131 a           61 c           60 g          111 t
ORIGIN      
        1 atgattgatg cctacatcac aacaaaaact gatttaacaa atggttggtc tgccttagaa
       61 agtatatttg aacattatct tgattatatt attgataata ataaaaacct tatccctatc
      121 caagaagtga tgcctatcat tggttggaat gaacttgaaa aaattagcct tgaatacatt
      181 actggtaagg taaacgccat tgtcagcaaa ttgatccaag agaaccaact taaagcttat
      241 gatgatgatg tgcttaaaaa cttactcaat ggctggttta tgcatatcgc aatacatgcg
      301 aaaaacctaa aagagcttgc cgataaaaaa ggccaattta ttgctattta ccgcggcttt
      361 tta
//
LOCUS       DAZPDE010000534          360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPDE010000534 DAZPDE010000000
VERSION     DAZPDE010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.542"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(126..335)
                     /gene="fumD"
                     /locus_tag="ACHU0J_004493"
     CDS             complement(126..335)
                     /gene="fumD"
                     /locus_tag="ACHU0J_004493"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1218809.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT           94 a           90 c           60 g          116 t
ORIGIN      
        1 atcgctataa attaaaatac ataccgaaat tcgtataatt tcagcagttg ttatttagaa
       61 tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg cctcatcctg
      121 aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc cagctcactg
      181 cgctgaatgc gtttattggc taatgctgta cgtaatacgc ctgcgataac aatatgcttt
      241 ggttcctgcc ccagatcgcg catttccagc acaactttac cgacaactct gcacatttct
      301 cggtacagtt catcctcttt tgtccgattt cccattcttt atacctattt atcatgccaa
//
LOCUS       DAZPDE010000535          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPDE010000535 DAZPDE010000000
VERSION     DAZPDE010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.denovo.543"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            39..>357
                     /locus_tag="ACHU0J_004494"
     CDS             39..>357
                     /locus_tag="ACHU0J_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1218810.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAZPDE010000536         9712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.141, whole genome shotgun sequence.
ACCESSION   DAZPDE010000536 DAZPDE010000000
VERSION     DAZPDE010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.141"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            complement(35..205)
                     /locus_tag="ACHU0J_004495"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACHU0J_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHU0J_004496"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHU0J_004496"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1218811.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACHU0J_004497"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACHU0J_004497"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1218812.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACHU0J_004498"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACHU0J_004498"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1218813.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACHU0J_004499"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACHU0J_004499"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1218814.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACHU0J_004500"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACHU0J_004500"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1218815.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6232
                     /locus_tag="ACHU0J_004501"
                     /pseudo
     CDS             5100..6232
                     /locus_tag="ACHU0J_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHU0J_004502"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHU0J_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1218816.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHU0J_004503"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHU0J_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1218817.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHU0J_004504"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHU0J_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1218818.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHU0J_004505"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHU0J_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1218819.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHU0J_004506"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHU0J_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1218820.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="ACHU0J_004507"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="ACHU0J_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1218821.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2484 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     7621 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     9481 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     9541 ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct ttgcacgttg
     9601 cataaccttt aattattcat atgcattgct gttgttactt tttatatagc taacaccaat
     9661 aattaacctt tcttttcggt aatgactcca acttactgat agtgttttat gt
//
LOCUS       DAZPDE010000537         8792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.154, whole genome shotgun sequence.
ACCESSION   DAZPDE010000537 DAZPDE010000000
VERSION     DAZPDE010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.154"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHU0J_004508"
     CDS             263..922
                     /locus_tag="ACHU0J_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1218822.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHU0J_004509"
     CDS             complement(1123..1500)
                     /locus_tag="ACHU0J_004509"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1218823.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHU0J_004510"
     CDS             complement(1567..4533)
                     /locus_tag="ACHU0J_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1218824.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHU0J_004511"
     CDS             complement(4536..5096)
                     /locus_tag="ACHU0J_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1218825.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHU0J_004512"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHU0J_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHU0J_004513"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHU0J_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1218826.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="ACHU0J_004514"
     CDS             6998..7828
                     /locus_tag="ACHU0J_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR1218827.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8779
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004515"
                     /pseudo
     CDS             7941..8779
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         2000 a         2357 c         2482 g         1953 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     8641 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     8701 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     8761 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZPDE010000538         8789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.155, whole genome shotgun sequence.
ACCESSION   DAZPDE010000538 DAZPDE010000000
VERSION     DAZPDE010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.155"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHU0J_004516"
     CDS             263..922
                     /locus_tag="ACHU0J_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR1218828.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHU0J_004517"
     CDS             complement(1123..1500)
                     /locus_tag="ACHU0J_004517"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1218829.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="ACHU0J_004518"
     CDS             complement(1567..4533)
                     /locus_tag="ACHU0J_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR1218830.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="ACHU0J_004519"
     CDS             complement(4536..5096)
                     /locus_tag="ACHU0J_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1218831.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="ACHU0J_004520"
                     /pseudo
     CDS             complement(5222..5572)
                     /locus_tag="ACHU0J_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHU0J_004521"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="ACHU0J_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR1218832.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="ACHU0J_004522"
     CDS             6998..7828
                     /locus_tag="ACHU0J_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR1218833.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8776
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004523"
                     /pseudo
     CDS             7941..8776
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1997 a         2357 c         2482 g         1953 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac
     8641 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     8701 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     8761 tcgcggcgcg gcttaactca agcgttaga
//
LOCUS       DAZPDE010000539         6780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.187, whole genome shotgun sequence.
ACCESSION   DAZPDE010000539 DAZPDE010000000
VERSION     DAZPDE010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.187"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHU0J_004524"
     CDS             59..1102
                     /locus_tag="ACHU0J_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1218834.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHU0J_004525"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHU0J_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHU0J_004526"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHU0J_004526"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1218835.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHU0J_004527"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHU0J_004527"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1218836.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHU0J_004528"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHU0J_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1218837.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACHU0J_004529"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACHU0J_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1218838.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..>6780)
                     /gene="phoE"
                     /locus_tag="ACHU0J_004530"
     CDS             complement(5846..>6780)
                     /gene="phoE"
                     /locus_tag="ACHU0J_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1218839.1"
                     /translation="HYMSDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNK
                     AESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNF
                     MTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSD
                     FAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITG
                     GFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKN
                     MSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         1637 a         1682 c         1769 g         1692 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
//
LOCUS       DAZPDE010000540         4295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.240, whole genome shotgun sequence.
ACCESSION   DAZPDE010000540 DAZPDE010000000
VERSION     DAZPDE010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.240"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHU0J_004531"
     CDS             558..797
                     /locus_tag="ACHU0J_004531"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1218840.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHU0J_004532"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHU0J_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHU0J_004533"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHU0J_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1218841.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHU0J_004534"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHU0J_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1218842.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004535"
                     /pseudo
     CDS             3444..4282
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAZPDE010000541         4292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.241, whole genome shotgun sequence.
ACCESSION   DAZPDE010000541 DAZPDE010000000
VERSION     DAZPDE010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.241"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            558..797
                     /locus_tag="ACHU0J_004536"
     CDS             558..797
                     /locus_tag="ACHU0J_004536"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR1218843.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHU0J_004537"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACHU0J_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHU0J_004538"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACHU0J_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR1218844.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACHU0J_004539"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACHU0J_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR1218845.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004540"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACHU0J_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAZPDE010000542         2406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2207980
            SAMN43277677-rid23488543.guided.309, whole genome shotgun sequence.
ACCESSION   DAZPDE010000542 DAZPDE010000000
VERSION     DAZPDE010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277677
            Sequence Read Archive: SRR30464184
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396535.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396535.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:35:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,450
            Genes (coding)                    :: 3,963
            CDSs (with protein)               :: 3,963
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 487
            CDSs (without protein)            :: 487
            Pseudo Genes (ambiguous residues) :: 0 of 487
            Pseudo Genes (frameshifted)       :: 226 of 487
            Pseudo Genes (incomplete)         :: 162 of 487
            Pseudo Genes (internal stop)      :: 167 of 487
            Pseudo Genes (multiple problems)  :: 64 of 487
            Pseudo Genes (short protein)      :: 1 of 487
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277677-rid23488543.guided.309"
                     /strain="M2207980"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-11-01"
                     /collected_by="FSS"
     gene            41..430
                     /locus_tag="ACHU0J_004541"
                     /pseudo
     CDS             41..430
                     /locus_tag="ACHU0J_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHU0J_004542"
     CDS             complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHU0J_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR1218846.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHU0J_004543"
     CDS             1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHU0J_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR1218847.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLSLYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          613 a          470 c          522 g          801 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aaccatggcg tacatgccaa
       61 agtttccacg ccagtgcatt tacgaaaagc acgtacttgc tatgatcatt tagctggcga
      121 agttgccgtt aagatctatg attccctttg tcaacagcaa tggatcactg aaaatggttc
      181 aatgatcaca ttaagtggta ttcaatattt tcatgaaatg ggaattgacg ttccttccaa
      241 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
      301 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      361 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      421 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      481 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      541 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      601 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      661 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      721 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      781 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      841 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      901 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      961 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     1021 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
     1081 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
     1141 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
     1201 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg ctttcacttt
     1261 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1321 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1381 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1441 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1501 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1561 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1621 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1681 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1741 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1801 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1861 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1921 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1981 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     2041 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     2101 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     2161 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     2221 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2281 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2341 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2401 agattt
//
